Insect Systematics & Evolution 46 (2015) 269–290 brill.com/ise The phylogenetic conundrum of Lutzia (Diptera: Culicidae: Culicini): a cautionary account of conflict and support Ian J. Kitching, C. Lorna Culverwell and Ralph E. Harbach* Department of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, UK *Corresponding author, e-mail:
[email protected] Published online 12 May 2014; published online 10 June 2015 Abstract Lutzia Theobald was reduced to a subgenus ofCulex in 1932 and was treated as such until it was restored to its original generic status in 2003, based mainly on modifications of the larvae for predation. Previous phylogenetic studies based on morphological and molecular data have provided conflicting support for the generic status of Lutzia: analyses of morphological data support the generic status whereas analyses based on DNA sequences do not. Our previous phylogenetic analyses of Culicini (based on 169 morpho- logical characters and 86 species representing the four genera and 26 subgenera of Culicini, most informal group taxa of subgenus Culex and five outgroup species from other tribes) seemed to indicate a conflict between adult and larval morphological data. Hence, we conducted a series of comparative and data exclu- sion analyses to determine whether the alternative positions of Lutzia are due to conflicting signal or to a lack of strong signal. We found that separate and combined analyses of adult and larval data support dif- ferent patterns of relationships between Lutzia and other Culicini. However, the majority of conflicting clades are poorly supported and once these are removed from consideration, most of the topological dis- parity disappears, along with much of the resolution, suggesting that morphology alone does not have sufficiently strong signal to resolve the position ofLutzia .