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Mosquito Species Identification Using Convolutional Neural Networks With
www.nature.com/scientificreports OPEN Mosquito species identifcation using convolutional neural networks with a multitiered ensemble model for novel species detection Adam Goodwin1,2*, Sanket Padmanabhan1,2, Sanchit Hira2,3, Margaret Glancey1,2, Monet Slinowsky2, Rakhil Immidisetti2,3, Laura Scavo2, Jewell Brey2, Bala Murali Manoghar Sai Sudhakar1, Tristan Ford1,2, Collyn Heier2, Yvonne‑Marie Linton4,5,6, David B. Pecor4,5,6, Laura Caicedo‑Quiroga4,5,6 & Soumyadipta Acharya2* With over 3500 mosquito species described, accurate species identifcation of the few implicated in disease transmission is critical to mosquito borne disease mitigation. Yet this task is hindered by limited global taxonomic expertise and specimen damage consistent across common capture methods. Convolutional neural networks (CNNs) are promising with limited sets of species, but image database requirements restrict practical implementation. Using an image database of 2696 specimens from 67 mosquito species, we address the practical open‑set problem with a detection algorithm for novel species. Closed‑set classifcation of 16 known species achieved 97.04 ± 0.87% accuracy independently, and 89.07 ± 5.58% when cascaded with novelty detection. Closed‑set classifcation of 39 species produces a macro F1‑score of 86.07 ± 1.81%. This demonstrates an accurate, scalable, and practical computer vision solution to identify wild‑caught mosquitoes for implementation in biosurveillance and targeted vector control programs, without the need for extensive image database development for each new target region. Mosquitoes are one of the deadliest animals in the world, infecting between 250–500 million people every year with a wide range of fatal or debilitating diseases, including malaria, dengue, chikungunya, Zika and West Nile Virus1. -
And Gecarcoidea Lalandii H. Milne
Journal of Natural History, 2002, 36, 1671–1685 First zoealstages of Epigrapsuspolitus Heller, E. notatus (Heller)and Gecarcoidealalandii H.Milne-Edwards, with remarks on zoeal morphologyof the Gecarcinidae Macleay (Crustacea: Brachyura) JOSE´ A.CUESTA†, HUNG-CHANG LIU‡and CHRISTOPH D. SCHUBART†1 †Department ofBiology, Laboratory for CrustaceanResearch, University ofLouisianaat Lafayette,Lafayette, LA 70504-2451,USA; e-mail: [email protected] ‡Department ofLife Science, National Tsing Hua University, Hsinchu, Taiwan (Accepted10 April 2001 ) Thecrab family Gecarcinidae Macleay, 1838 currently consists of 18speciesthat aregrouped in four genera. Larval data for the Gecarcinidae were only known forspecies of the genera Cardisoma Latreille,1825 and Gecarcinus Leach,1814. Inthe present paper, the rstzoeal stage of Gecarcoidealalandii H. Milne- Edwards,1837, Epigrapsuspolitus Heller,1862 and E. notatus Heller,1865 are describedand illustrated. Zoeal morphology of the Gecarcinidae is reviewed, takinginto account all previous descriptions and analysing the relationships betweenthe di Verentgenera of Gecarcinidae based on zoeal morphological characters.A seriesof typical morphological features is proposed for the zoea larvaeof this family di Verentiatingthem from the rest of the Grapsoidea. Keywords: Larvalmorphology, Gecarcinidae, Gecarcoidea , Epigrapsus, zoea. Introduction The grapsoid familyGecarcinidae has acircumtropical distribution, with many species known only from oceanic islands. The familypresently consists of 18species that aredistributed -
Data-Driven Identification of Potential Zika Virus Vectors Michelle V Evans1,2*, Tad a Dallas1,3, Barbara a Han4, Courtney C Murdock1,2,5,6,7,8, John M Drake1,2,8
RESEARCH ARTICLE Data-driven identification of potential Zika virus vectors Michelle V Evans1,2*, Tad A Dallas1,3, Barbara A Han4, Courtney C Murdock1,2,5,6,7,8, John M Drake1,2,8 1Odum School of Ecology, University of Georgia, Athens, United States; 2Center for the Ecology of Infectious Diseases, University of Georgia, Athens, United States; 3Department of Environmental Science and Policy, University of California-Davis, Davis, United States; 4Cary Institute of Ecosystem Studies, Millbrook, United States; 5Department of Infectious Disease, University of Georgia, Athens, United States; 6Center for Tropical Emerging Global Diseases, University of Georgia, Athens, United States; 7Center for Vaccines and Immunology, University of Georgia, Athens, United States; 8River Basin Center, University of Georgia, Athens, United States Abstract Zika is an emerging virus whose rapid spread is of great public health concern. Knowledge about transmission remains incomplete, especially concerning potential transmission in geographic areas in which it has not yet been introduced. To identify unknown vectors of Zika, we developed a data-driven model linking vector species and the Zika virus via vector-virus trait combinations that confer a propensity toward associations in an ecological network connecting flaviviruses and their mosquito vectors. Our model predicts that thirty-five species may be able to transmit the virus, seven of which are found in the continental United States, including Culex quinquefasciatus and Cx. pipiens. We suggest that empirical studies prioritize these species to confirm predictions of vector competence, enabling the correct identification of populations at risk for transmission within the United States. *For correspondence: mvevans@ DOI: 10.7554/eLife.22053.001 uga.edu Competing interests: The authors declare that no competing interests exist. -
Centro De Investigación En Alimentación Y Desarrollo A
CENTRO DE INVESTIGACIÓN EN ALIMENTACIÓN Y DESARROLLO A. C. ASPECTOS ECOLÓGICOS Y MOLECULARES DEL CANGREJO TERRESTRE Johngarthia planata (STIMPSON, 1860) EN LA ISLA SAN PEDRO NOLASCO, SONORA, MÉXICO Por: Ana Gabriela Martínez Vargas TESIS APROBADA POR LA: COORDINACIÓN DE ASEGURAMIENTO DE CALIDAD Y APROVECHAMIENTO SUSTENTABLE DE RECURSOS NATURALES Como requisito para obtener el grado de: MAESTRA EN CIENCIAS Guaymas, Sonora Enero de 2015 APROBACIÓN ii DECLARACIÓN INSTITUCIONAL La información generada en esta tesis es propiedad intelectual del Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD). Se permiten y agradecen las citas breves del material contenido en esta tesis sin permiso especial del autor, siempre y cuando se dé crédito correspondiente. Para la reproducción parcial o total de la tesis con fines académicos, se deberá contar con la autorización escrita del Director General del CIAD. La publicación en comunicaciones científicas o de divulgación popular de los datos contenidos en esta tesis, deberá dar los créditos al CIAD, previa autorización escrita del manuscrito en cuestión del director de tesis. ______________________________ Dr. Pablo Wong González Director General iii AGRADECIMIENTOS A CONACYT por otorgarme la beca, la cual fue indispensable para la realización de este trabajo. A CIAD, A.C. Unidad Guaymas por permitirme utilizar sus instalaciones para realizar mi tesis de maestría, ayudándome en esta etapa a cumplir con mi preparación profesional. En particular al Dr. Juan Pablo Gallo por aceptarme y permitirme formar parte de su equipo de trabajo. A CIAD, A.C. Unidad Mazatlán, en especial a mi asesora Dra. Alejandra García Gasca por permitirme usar el laboratorio de Biología Molecular y a la técnico Rubí Hernández por enseñarme las técnicas y manejo de los equipos para realizar los estudios moleculares requeridos en mi tesis. -
17 the Crabs Belonging to the Grapsoidea Include a Lot Of
17 SUPERFAMILY GRAPSOIDEA The crabs belonging to the Grapsoidea include a lot of ubiquitous species collected in the mangrove and/or along the coastline. As a result, most of the species listed here under the ‘Coastal Rock-rubble’ biotope of table 2b could be reasonably listed also with marine species. This is particularly true for the Grapsidae: Grapsus, Pachygrapsus, Pseudograpsus, and Thalassograpsus. FAMILY GECARCINIDAE Cardisoma carnifex (Herbst, 1796). Figure 12. – Cardisoma carnifex - Guinot, 1967: 289 (Checklist of WIO species, with mention of Grande Comore and Mayotte). - Bouchard, 2009: 6, 8, Mayotte, Malamani mangrove, 16 April 2008, St. 1, 12°55.337 S, 44°09.263 E, upper mangrove in shaded area, burrow, about 1.5 m depth, 1 male 61×74 mm (MNHN B32409). - KUW fieldwork November 2009, St. 6, Petite Terre, Badamiers spillway, upper littoral, 1 female 53×64 mm (MNHN B32410), 1 male 65×75.5 mm (MNHN B32411); St. 29, Ngouja hotel, Mboianatsa beach, in situ photographs only. Distribution. – Widespread in the IWP. Red Sea, Somalia, Kenya, Tanzania, Mozambique, South Africa, Europa, Madagascar, Comoros, Seychelles, Réunion, Mauritius, India, Taiwan, Japan, Australia, New Caledonia, Fiji, Wallis & Futuna, French Polynesia. Comment. – Gecarcinid land crabs are of large size and eaten in some places (West Indies, Wallis & Futuna, and French Polynesia). In Mayotte, however, they are not much prized for food and are not eaten. Figure 12. Cardisoma carnifex. Mayotte, KUW 2009 fieldwork: A) aspect of station 29, upper littoral Ngouja hotel, Mboianatsa beach; B) same, detail of a crab at the entrance of its burrow; C) St. 6, 1 female 53×64 mm (MNHN B32410); D) probably the same specimen, in situ at St. -
Dry Season Distribution of Land Crabs, Gecarcinus Quadratus (Crustacea: Gecarcinidae), in Corcovado National Park, Costa Rica
Dry season distribution of land crabs, Gecarcinus quadratus (Crustacea: Gecarcinidae), in Corcovado National Park, Costa Rica Megan E. Griffiths1,2, Basma A. Mohammad1,3 & Andres Vega4 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Present Address: Forest Biodiversity Research Unit, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, Pietermaritzburg, South Africa; [email protected] 3 Monterey Institute of International Studies, Monterey, CA 93940, USA. 4 Ambiente, Biología, e Investigación en Corcovado, Estación Biológica Sirena, Parque Nacional Corcovado, Costa Rica Received 12-VII-2004. Corrected 19-VII-2006. Accepted 15-XII-2006. Abstract: The land crab Gecarcinus quadratus is an engineering species that controls nutrient cycling in tropical forests. Factors regulating their coastal distribution are not fully understood. We quantified land crab distribution during the dry season at Sirena Field Station in Corcovado National Park, Costa Rica, and found that land crab burrow density decreases with increasing distance from the ocean. Leaf litter depth and tree seedling density are negatively correlated with land crab burrow density. Burrows are strongly associated with sand substrate and burrow density is comparatively low in clay substrate. Results suggest that G. quadratus is limited to a narrow coastal zone with sand substrate, and this distribution could have profound effects on plant community structure. Rev. Biol. Trop. 55 (1): 219-224. Epub 2007 March. 31. Key words: burrow counts, Corcovado National Park, Costa Rica, Gecarcinus quadratus, Gecarcinus lateralis, land crabs, leaf litter. Recent studies have called attention to While previous studies have described the influence that land crabs have on coastal the ranges of land crab populations on islands ecosystems (Green et al. -
MOSQUITOES of the SOUTHEASTERN UNITED STATES
L f ^-l R A R > ^l^ ■'■mx^ • DEC2 2 59SO , A Handbook of tnV MOSQUITOES of the SOUTHEASTERN UNITED STATES W. V. King G. H. Bradley Carroll N. Smith and W. C. MeDuffle Agriculture Handbook No. 173 Agricultural Research Service UNITED STATES DEPARTMENT OF AGRICULTURE \ I PRECAUTIONS WITH INSECTICIDES All insecticides are potentially hazardous to fish or other aqpiatic organisms, wildlife, domestic ani- mals, and man. The dosages needed for mosquito control are generally lower than for most other insect control, but caution should be exercised in their application. Do not apply amounts in excess of the dosage recommended for each specific use. In applying even small amounts of oil-insecticide sprays to water, consider that wind and wave action may shift the film with consequent damage to aquatic life at another location. Heavy applications of insec- ticides to ground areas such as in pretreatment situa- tions, may cause harm to fish and wildlife in streams, ponds, and lakes during runoff due to heavy rains. Avoid contamination of pastures and livestock with insecticides in order to prevent residues in meat and milk. Operators should avoid repeated or prolonged contact of insecticides with the skin. Insecticide con- centrates may be particularly hazardous. Wash off any insecticide spilled on the skin using soap and water. If any is spilled on clothing, change imme- diately. Store insecticides in a safe place out of reach of children or animals. Dispose of empty insecticide containers. Always read and observe instructions and precautions given on the label of the product. UNITED STATES DEPARTMENT OF AGRICULTURE Agriculture Handbook No. -
Present Status of Cardisoma Guanhumi Latreille, 1828 (Crustacea: Brachyura: Gecarcinidae) Populations in Venezuela
PreseNT STATUS OF Cardisoma guanhumi Latreille, 1828 (Crustacea: Brachyura: Gecarcinidae) popULATIONS IN VENEZUELA CArlos CARMONA-SUÁreZ SUMMARY Venezuela has a large coastal strip with marine and estuarine ties in each of the sampling periods. Grand mean densities were environments, especially mangrove ecosystems that are adequate 2.18 burrows/m² in November- January and 1.6 in June-July. No for populations of the land crab Cardisoma guanhumi. This crab significant density differences were found between sampling pe- is commercially exploited in our country. Data on its distribution riods nor between regions. Burrow diameters were significantly and abundance is restricted and outdated. Due to its ecological different between sampling periods, being larger in June-July en economical importance, the goal of this work is to determine (65.66mm), and were significantly different between western and the present population status. Twenty locations were sampled central, and between western and eastern regions. Burrow densi- along approximately 75% of the Venezuelan coasts in two sam- ties were higher than those found in Puerto Rico and were also pling periods (November 2009-January 2010, and June-July higher with densities reported for the Tucacas-Boca de Aroa 2010). Population density was estimated by counting occupied area by other authors. The high densities of C. guanhumi in burrows within a quadrate of 80m² in each of the selected loca- Venezuela indicate that these populations are possible not endan- tions, and burrow diameters were measured in each of the locali- gered by their present commercial exploitation. and crabs have acquired dhaus et al., 2009). One topic that has lately entific studies (Gifford, 1963; Herreid and such an important rele- been intensively treated is the manner how Gifford, 1963; Taissoun, 1974; Giménez and vance in biology, that a to measure population density and animal Acevedo, 1982; Burggren et al., 1985; Wol- whole book has been dedicated to them size in land crabs by using indirect non- cott and Wolcott, 1987, among others). -
Endemic Species of Christmas Island, Indian Ocean D.J
RECORDS OF THE WESTERN AUSTRALIAN MUSEUM 34 055–114 (2019) DOI: 10.18195/issn.0312-3162.34(2).2019.055-114 Endemic species of Christmas Island, Indian Ocean D.J. James1, P.T. Green2, W.F. Humphreys3,4 and J.C.Z. Woinarski5 1 73 Pozieres Ave, Milperra, New South Wales 2214, Australia. 2 Department of Ecology, Environment and Evolution, La Trobe University, Melbourne, Victoria 3083, Australia. 3 Western Australian Museum, Locked Bag 49, Welshpool DC, Western Australia 6986, Australia. 4 School of Biological Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia. 5 NESP Threatened Species Recovery Hub, Charles Darwin University, Casuarina, Northern Territory 0909, Australia, Corresponding author: [email protected] ABSTRACT – Many oceanic islands have high levels of endemism, but also high rates of extinction, such that island species constitute a markedly disproportionate share of the world’s extinctions. One important foundation for the conservation of biodiversity on islands is an inventory of endemic species. In the absence of a comprehensive inventory, conservation effort often defaults to a focus on the better-known and more conspicuous species (typically mammals and birds). Although this component of island biota often needs such conservation attention, such focus may mean that less conspicuous endemic species (especially invertebrates) are neglected and suffer high rates of loss. In this paper, we review the available literature and online resources to compile a list of endemic species that is as comprehensive as possible for the 137 km2 oceanic Christmas Island, an Australian territory in the north-eastern Indian Ocean. -
Checklist of Brachyuran Crabs (Crustacea: Decapoda) from the Eastern Tropical Pacific by Michel E
BULLETIN DE L'INSTITUT ROYAL DES SCIENCES NATURELLES DE BELGIQUE, BIOLOGIE, 65: 125-150, 1995 BULLETIN VAN HET KONINKLIJK BELGISCH INSTITUUT VOOR NATUURWETENSCHAPPEN, BIOLOGIE, 65: 125-150, 1995 Checklist of brachyuran crabs (Crustacea: Decapoda) from the eastern tropical Pacific by Michel E. HENDRICKX Abstract Introduction Literature dealing with brachyuran crabs from the east Pacific When available, reliable checklists of marine species is reviewed. Marine and brackish water species reported at least occurring in distinct geographic regions of the world are once in the Eastern Tropical Pacific zoogeographic subregion, of multiple use. In addition of providing comparative which extends from Magdalena Bay, on the west coast of Baja figures for biodiversity studies, they serve as an impor- California, Mexico, to Paita, in northern Peru, are listed and tant tool in defining extension of protected area, inferr- their distribution range along the Pacific coast of America is provided. Unpublished records, based on material kept in the ing potential impact of anthropogenic activity and author's collections were also considered to determine or con- complexity of communities, and estimating availability of firm the presence of species, or to modify previously published living resources. Checklists for zoogeographic regions or distribution ranges within the study area. A total of 450 species, provinces also facilitate biodiversity studies in specific belonging to 181 genera, are included in the checklist, the first habitats, which serve as points of departure for (among ever made available for the entire tropical zoogeographic others) studying the structure of food chains, the relative subregion of the west coast of America. A list of names of species abundance of species, and number of species or total and subspecies currently recognized as invalid for the area is number of organisms of various physical sizes (MAY, also included. -
Territorial Behavior in Dominican Land Crabs Gecarcinus Ruricola and Gecarcinus Lateralis • Angelica Biernat
Territorial Behavior in Dominican Land Crabs Gecarcinus ruricola and Gecarcinus lateralis • Angelica Biernat Dominica Study Abroad 2000 Dr. Tom Lacher and Dr. Jim Woolley Texas A&M University • , • Abstract This project consisted of observations of Gecarcinus ruricola and Gecarcinus lateralis. It was determined that both species are highly territorial and appear to establish specific holes to which they repeatedly return. It is possible that a chemical marker is used to locate the precise hole. Introduction My study was conducted through observations of both Gecarcinus ruricola and Gecarcinus lateralis land crabs on the island of Dominica in the West Indies. G. ruricola is more commonly known as the Black Crab. It is darker in color and is the • larger of the two at 2.8 inches. It is also the most common crab to be used as food on Dominica. In contrast, G. lateralis, locally referred to as "Totoloo," is more reddish-brown in color and only grows to about 1.8 inches. Both crabs are relatively nocturnal and highly terrestrial. Unfortunately, neither species has been the subject of extensive research. Thus, there were many interesting options to pursue, but the purpose of my study became focused on observing the territorial behaviors of these land crabs. Materials * Thick rubber gloves • * Buckets of varying sizes and depths * Fingernail polishes * Tagging ribbon Methods It was discovered that the crabs were most active just after a rain and were easily captured along the Imperial Highway and along the pathway below the Stream House at Springfield. The crabs were simply collected by hand and were placed in separate buckets according to size. -
Diptera: Culicidae: Culicini): a Cautionary Account of Conflict and Support
Insect Systematics & Evolution 46 (2015) 269–290 brill.com/ise The phylogenetic conundrum of Lutzia (Diptera: Culicidae: Culicini): a cautionary account of conflict and support Ian J. Kitching, C. Lorna Culverwell and Ralph E. Harbach* Department of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, UK *Corresponding author, e-mail: [email protected] Published online 12 May 2014; published online 10 June 2015 Abstract Lutzia Theobald was reduced to a subgenus ofCulex in 1932 and was treated as such until it was restored to its original generic status in 2003, based mainly on modifications of the larvae for predation. Previous phylogenetic studies based on morphological and molecular data have provided conflicting support for the generic status of Lutzia: analyses of morphological data support the generic status whereas analyses based on DNA sequences do not. Our previous phylogenetic analyses of Culicini (based on 169 morpho- logical characters and 86 species representing the four genera and 26 subgenera of Culicini, most informal group taxa of subgenus Culex and five outgroup species from other tribes) seemed to indicate a conflict between adult and larval morphological data. Hence, we conducted a series of comparative and data exclu- sion analyses to determine whether the alternative positions of Lutzia are due to conflicting signal or to a lack of strong signal. We found that separate and combined analyses of adult and larval data support dif- ferent patterns of relationships between Lutzia and other Culicini. However, the majority of conflicting clades are poorly supported and once these are removed from consideration, most of the topological dis- parity disappears, along with much of the resolution, suggesting that morphology alone does not have sufficiently strong signal to resolve the position ofLutzia .