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(MMP10) Moderates Inflammation by Controlling Macrophage Activation

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(MMP10) Moderates Inflammation by Controlling Macrophage Activation Stromelysin-2 (MMP10) Moderates Inflammation by Controlling Macrophage Activation This information is current as Ryan S. McMahan, Timothy P. Birkland, Kate S. Smigiel, of September 26, 2021. Tyler C. Vandivort, Maryam G. Rohani, Anne M. Manicone, John K. McGuire, Sina A. Gharib and William C. Parks J Immunol 2016; 197:899-909; Prepublished online 17 June 2016; doi: 10.4049/jimmunol.1600502 Downloaded from http://www.jimmunol.org/content/197/3/899 Supplementary http://www.jimmunol.org/content/suppl/2016/06/16/jimmunol.160050 Material 2.DCSupplemental http://www.jimmunol.org/ References This article cites 72 articles, 15 of which you can access for free at: http://www.jimmunol.org/content/197/3/899.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision by guest on September 26, 2021 • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2016 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology Stromelysin-2 (MMP10) Moderates Inflammation by Controlling Macrophage Activation Ryan S. McMahan,*,† Timothy P. Birkland,*,‡ Kate S. Smigiel,x Tyler C. Vandivort,*,†,x Maryam G. Rohani,*,‡,x Anne M. Manicone,*,‡ John K. McGuire,*,{ Sina A. Gharib,*,‡ and William C. Parks*,‡,x Several members of the matrix metalloproteinase (MMP) family control a range of immune processes, such as leukocyte influx and chemokine activity. Stromelysin-2 (MMP10) is expressed by macrophages in numerous tissues after injury; however, little is known of its function. In this study, we report that MMP10 is expressed by macrophages in human lungs from patients with cystic fibrosis and induced in mouse macrophages in response to Pseudomonas aeruginosa infection both in vivo and by isolated resident alveolar and bone marrow–derived macrophages (BMDM). Our data indicates that macrophage MMP10 serves a beneficial function in response to acute infection. Whereas wild-type mice survived infection with minimal morbidity, 50% of Mmp102/2 mice died and Downloaded from all showed sustained weight loss (morbidity). Although bacterial clearance and neutrophil influx did not differ between genotypes, macrophage numbers were ∼3-fold greater in infected Mmp102/2 lungs than in wild-types. Adoptive transfer of wild-type BMDM normalized infection-induced morbidity in Mmp102/2 recipients to wild-type levels, demonstrating that the protective effect of MMP10 was due to its production by macrophages. Both in vivo and in cultured alveolar macrophages and BMDM, expression of several M1 macrophage markers was elevated, whereas M2 markers were reduced in Mmp102/2 tissue and cells. Global gene expression analysis revealed that infection-mediated transcriptional changes persisted in Mmp102/2 BMDM long after they http://www.jimmunol.org/ were downregulated in wild-type cells. These results indicate that MMP10 serves a beneficial role in response to acute infection by moderating the proinflammatory response of resident and infiltrating macrophages. The Journal of Immunology, 2016, 197: 899–909. acrophages are critical effector cells of the immune macrophages are induced by infection and proinflammatory Th1 system and play essential, yet distinct, roles in both cytokines, are effective at killing bacteria, and release proin- M promoting and resolving inflammation and in facili- flammatory cytokines, such as IL-1b, IL-12, and TNF-a.M2 tating tissue repair and contributing to its destruction (1). That a macrophages are induced by the Th2 cytokines IL-4 and IL-13 by guest on September 26, 2021 single cell type can serve opposing functions may seem counter- and other factors, release anti-inflammatory factors, such as IL- intuitive, but dramatic phenotypic changes occur when macro- 10, are weakly microbicidal, and promote repair. We recognize, phages respond to local stimuli (1–4). Based on patterns of gene however, that dividing macrophages into M1 and M2 classes and protein expression and function, macrophages are commonly oversimplifies the complex continuum of functional and revers- classified as classically activated (M1) or alternatively activated ible states in which these cells exist (6–9). (M2) cells, as well as a variety of M2 subtypes (1, 4, 5). M1 Several proteins influence macrophage behavior, including some members of the matrix metalloproteinase (MMP) gene family. For example, MMP12 and MMP28, both macrophage products, *Center for Lung Biology, University of Washington, Seattle, WA 98109; either promote or restrict macrophage influx into lung (10, 11), and †Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA 98105; ‡Department of Medicine, University of Washing- MMP8 promotes M2 polarization (12). Additionally, we reported ton, Seattle, WA 98195; xWomen’s Guild Lung Institute, Cedars–Sinai Medical Cen- that MMP28 and TIMP3, an MMP inhibitor, moderate M1 acti- { ter, Los Angeles, CA 90048; and Department of Pediatrics, University of Washington, vation of macrophages in models of lung infection and fibrosis Seattle, WA 98195 (13, 14). As their name implies, MMPs are thought to degrade ORCIDs: 0000-0003-3489-6127 (R.S.M.); 0000-0003-0475-2923 (T.C.V.); 0000- 0001-6106-8349 (J.K.M.). extracellular matrix proteins, a function that is indeed performed Received for publication March 23, 2016. Accepted for publication May 24, 2016. by some members (15–17). However, matrix degradation is nei- ther the shared nor predominant function of these enzymes. This work was supported by National Institutes of Health Grants HL089455, HL128995, and HL098067 (to W.C.P.), HL093022 (to J.K.M.), and HL116514 (to A.M.M.). Rather, individual MMPs have been shown to regulate specific The microarray experiments–compliant data presented in this article have been sub- immune processes, such as leukocyte influx and activation, anti- mitted to the National Institutes of Health Gene Expression Omnibus (http://www. microbial activity, and restoration of barrier function, typically by ncbi.nlm.nih.gov/geo/query/acc.cgi?token=epkvmssmvhmrlmf&acc=GSE78175) un- der accession number GSE78175. processing of a range of non–matrix protein substrates (18–25). MMP10 (stromelysin-2) is not expressed in developing or Address correspondence and reprint requests to Dr. William C. Parks, Women’s Guild Lung Institute, Cedars–Sinai Medical Center, 8700 Beverly Boulevard, Los normal adult tissues, including lung (26, 27). However, in both Angeles, CA 90048. E-mail address: [email protected] human conditions and mouse models, MMP10 is induced in re- The online version of this article contains supplemental material. sponse to injury, infection, or transformation in essentially all Abbreviations used in this article: BAL, bronchoalveolar lavage; BMDM, bone marrow– tissues (28–33). In a meta-analysis of gene array experiments derived macrophage; CF, cystic fibrosis; DAB, 3,39-diaminobenzidine; DIG, digoxigenin; involving numerous different host–pathogen interactions, MMP10 iNOS, inducible NO synthase; MHC II, MHC class II; MMP, matrix metalloproteinase. was identified as a common host response gene (34). The wide- Copyright Ó 2016 by The American Association of Immunologists, Inc. 0022-1767/16/$30.00 spread expression of MMP10 among tissues suggests that this www.jimmunol.org/cgi/doi/10.4049/jimmunol.1600502 900 MMP10 SHAPES MACROPHAGE ACTIVATION proteinase serves critical roles in the host response to environ- Immunostaining and in situ hybridization mental insults. As we show in the present study, MMP10 serves Deidentified human lung specimens were obtained with approval of the a protective role in acute infection by moderating the proinflam- University of Washington Institutional Review Board. Sections (5 mm) matory activity of macrophages. were stained with Abs specific for human MMP10 (Abcam, Cambridge, Recently, we reported that macrophage MMP10 promotes the MA) as described (40). Mouse lungs were perfused-fixed with PBS- ability of M2 macrophages to clear scar tissues in normal skin buffered formalin via the trachea under 25-cm pressure for 5 min, in- cubated in fixative at room temperature for 48 h, dehydrated through wounds by controlling the expression of collagenolytic MMPs, graded ethanol, and embedded in paraffin. Deparaffinized sections (5 particularly MMP13 (35). However, because sterile excision mm) underwent heat-induced (95˚C, 30 min) Ag retrieval in pH 7.0 wounds are not associated with a profound inflammatory re- citrate. Endogenous peroxidase activity was quenched with 3% H2O2 for sponse, the stimulus (i.e., a clean wound) may not have been 10 min. Sections were incubated with 4% normal rabbit serum for 1 h and then overnight at 4˚C with anti-Mac2 Ab (1:500 dilution; clone M3/ sufficiently robust to reveal other MMP10-dependent roles in 38.1.2.8 HL.2, American Type Culture Collection, Manassas VA). Bound macrophage inflammation. Thus, for the present studies, we Ab was detected
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