BD Lyoplate™ Screening Panels Human Cell Surface Markers/Mouse Cell Surface Markers
Features BD Lyoplate™ screening panels are the first comprehensive systems available for the efficient profiling of hundreds of murine and human Support the efficient, cost-effective profiling of hundreds cell surface markers by flow cytometry or bioimaging. Deciphering of cell surface markers with purified antibodies, isotype the cell surface proteome (eg, of differentiating stem cells) will enable controls, and detection reagents researchers to define strategies for the analysis and isolation of Proprietary lyophilized format enables long-term room targeted cells from heterogenous populations for functional studies, drug discovery research, in vivo animal studies, and cell therapy temperature storage research. Convenient 96-well plate-based format for automated A cost-effective profile of cell surface proteins processing With hundreds of monoclonal antibodies (mAbs) in each panel, Expandable panel design provides open wells for BD Lyoplate cell surface marker screening panels provide one of the most cost-effective screening tools available for immunophenotyping additional biomarkers or positive controls tissues and individual, intact live and fixed cells. To simplify the Alexa Fluor® 647–conjugated detection reagents are transition to more targeted, scaled up experiments, all antibodies compatible with green fluorescent protein (GFP) and included in the screening panels are available in the BD Biosciences yellow fluorescent protein (YFP) expressing cells catalog as single-vial, unlabeled, or conjugated formats tested for a variety of applications, as well as in custom sizes and fluorochromes. Simplify workflow Each panel contains three plates. Each well contains lyophilized, purified antibody to one cell surface marker or isotype control. Following reconstitution, the antibody is incubated with cellular samples and then with the detection reagents included with the BD Lyoplate screening panel. Finally, samples are analyzed by flow cytometry or imaging. To provide flexibility while simplifying workflow, open wells allow for the panel to be expanded to include additional markers. Accelerate discovery Easy-to-use Microsoft® Excel® 2007 templates are available to organize FCS file data into a 96-well array format overlaid with the corresponding plate map (Figures 1 and 2). The templates are used to perform normalization to isotype controls and generate heatmaps illustrating the mean fluorescence intensity (MFI) or percentage of positive cells for each biomarker. Side-by-side comparative analyses of multiple screens (eg, cells in various states of differentiation or culture conditions) enable the identification of unique cell surface marker expression signatures that permit the characterization and isolation of distinct cell populations from heterogenous samples for downstream applications. A convenient format for greater productivity and stability The plate-based format is compatible with automation and multichannel pipetting, helping to reduce hands-on time and offering improved lab productivity. The proprietary lyophilized format offers at least two years of stability of the air-tight packaged plates at room temperature, saving space in refrigerated storage. Custom solutions The BD Custom Technology Team (CTT) offers custom BD Lyoplate™ technology as a unique product option for researchers looking for greater lab-to-lab consistency and less hands-on time. The lyophilized products are uniquely suited for multi-site or longitudinal flow cytometry and bioimaging studies. Designed for specific experiment requirements, custom lyophilized products can include antibodies, antibody conjugates, peptides, and control cells. Lyophilized products can be packaged in many formats such as tubes, 96-well plates, and single vials. CTT solutions can also expand beyond typical offerings to essential resources such as protocol development, flexible delivery options, and quality documentation.
Visit bdbiosciences.com/stemcell for more information, or for custom BD Lyoplate solutions visit bdbiosciences.com/ctt.
For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD Lyoplate™ Screening Panels Human and Mouse Cell Surface Markers
Figure 1. Array layout of the BD Lyoplate Human Cell Surface Figure 2. Array layout of the BD Lyoplate Mouse Cell Surface Marker Screening Panel. Marker Screening Panel. Plate 1 Plate 1
CD5 CD8a CD16/ Buffer CD1a CD1b CD1d CD2 CD3 CD4 CD4v4 CD5 CD6 CD7 CD8a Buffer CD2 CD4 (Ly-1) (Ly-2) CD9 CD11a CD11b CD13 CD14 CD32 CD18
CD21/ CD8b CD9 CD10 CD11a CD11b CD11c CD13 CD14 CD15 CD15s CD16 CD18 CD19 CD35 CD23 CD24 CD25 CD26 CD29 CD31 CD34 CD35 CD38 CD41
CD19 CD20 CD21 CD22 CD23 CD24 CD25 CD26 CD27 CD28 CD29 CD30 CD43 CD44 CD45 CD45R CD45RA CD45RC CD47 CD49b CD49d CD49e CD51 CD53
CD31 CD32 CD33 CD34 CD35 CD36 CD37 CD38 CD39 CD40 CD41a CD41b CD62E CD62L CD70 CD71 CD72b/c CD73 CD80 CD83 CD86 CD90.2 CD94 CD98
CD42a CD42b CD43 CD44 CD45 CD45RA CD45RB CD45RO CD46 CD47 CD48 CD49a CD102 CD103 CD104 CD106 CD117 CD121a CD121b CD122 CD123 CD124 CD125 CD126
CD49b CD49c CD49d CD49e CD50 CD51/61 CD53 CD54 CD55 CD56 CD57 CD58 CD127 CD131 CD132 CD134 CD135 CD137 CD138 CD140a CD144 CD147 CD153 CD162
CD66 CD184 CD185 CD59 CD61 CD62E CD62L CD62P CD63 CD64 (a,c,d,e) CD66b CD66f CD69 CD70 CD172a CD179a CD179b CD180 (CXCR4) (CXCR5) CD195 CD197 CD200 CD209a CD210 CD223
CD254 CD309 CD71 CD72 CD73 CD74 CD75 CD77 CD79b CD80 CD81 CD83 CD84 CD85 CD244.1 CD252 CD253 CD267 CD273 CD274 CD278 CD284 (Flk-1, CD314 CD326 (RANKL) VEGF-R2)
Plate 2 Plate 2
CD335 4-1BB Dendritic Early B F4/80-like IL-21 Integrin Buffer CD86 CD87 CD88 CD89 CD90 CD91 CDw93 CD94 CD95 CD97 CD98 Buffer Ligand Crry/p65 GITR I-A/I-E LPAM-1 (NKp46) (CD137L) Cells Lineage receptor Receptor b7 chain
Ly-6A/E Ly-6D Ly-6G MAC-3 MAd- MD-1 NKG2 NK-T/NK Panendo- CD99 CD99R CD100 CD102 CD103 CD105 CD106 CD107a CD107b CD108 CD109 CD112 Ly-6G Cell thelial Cell PIR-A/B Pre BCR (Sca-1) (ThB) Ly-6C (CD107b) CAM-1 (Ly-86) A/C/E Antigen Antigen
CD118 T/B cell TER-119 Siglec-F Syndecan Igλ CD114 CD116 CD117 (LIFR CD119 CD120a CD121a CD121b CD122 CD123 CD124 CD126 activation (Erythroid 123 IgD IgE IgM Buffer Buffer Buffer Buffer (Siglec5) 4 (Sdc4) light chain cptr) antigen Cells)
CD137 CD127 CD128b CD130 CD134 CD135 CD137 CD138 CD140a CD140b CD141 CD142 Rat Rat Rat Rat Rat Rat Rat Buffer Buffer Buffer Buffer Buffer Ligand IgG1, k IgG1, l IgG2a, k IgG2a, l IgG2b, k IgG2c, k IgM, k
CD144 CD146 CD147 CD150 CD151 CD152 CD153 CD154 CD158a CD158b CD161 CD162 Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer
CD163 CD164 CD165 CD166 CD171 CD172b CD177 CD178 CD180 CD181 CD183 CD184 Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer
CD193 CD195 CD196 CD197 CD200 CD205 CD206 CD209 CD220 CD221 CD226 CD227 Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer
CD243 CD255 CD275 (p-glyco CD229 CD231 CD235a CD244 (Tweak) CD268 CD271 CD273 CD274 (B7-H2) CD278 CD28 KLRG1 Vg 3 TCR Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Protein)
Plate 3 Plate 3
CD321 CD305 CD314 CD335 (F11 Buffer CD279 CD282 (LAIR-1) CD309 (NKG2D) CDw327 CDw328 CDw329 (NKP46) CD336 Buffer CD3e CD11c CD27 CD30 CD40 CD42d CD48 CD54 CD55 CD61 CD69 Rcptr)
CD338 CD340 b2micro EGF fMLP CD337 (ABCG2) (Her2) abTCR globulin BLTR-1 CLIP CMRF-44 CMRF-56 Receptor Receptor γδTCR CD79b CD81 CD95 CD119 CD120a CD120b CD152 CD154 CD279 gδ-TCR gp49 R H2-M3
Hem. HLA- Disialo- HLA- HLA- HLA- HLA- Invariant IFN-g TCR b Prog. DR,DP, ganglio- MIC A/B NKB1 SSEA-1 SSEA-4 Receptor b Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer A,B,C A2 DQ DR NKT chain Cell DQ side GD2 chain
Ham Ham Ham Ham Ham Ham TRA-1-60 TRA-1-81 V 23 V 8 CD326 Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer b b IgG1, k IgG1, l IgG2, k IgG2, l IgG3, l IgM, l
mlgM mlgG1 mlgG2a mlgG2b mlgG3 Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer
Cutaneous Integrin CD72 CD49f CD104 CD120b CD132 CD201 CD210 CD212 CD267 CD294 SSEA-3 Lymph. CD22.2 CD45.1 CD45.2 CD64a/b CD157 CD212 CD244.2 H-2Db H-2Kb H-2Kd H-2KK Antigen b7 a/b/d
IFN- / Ly-49C rlgM rlgG1 rlgG2a rlgG2b Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer H-2KQ H-2KS a b Ly-51 NK-1.1 Pre TCR QA-1B SSEA-1 SSEA-4 Buffer Buffer Receptor 1 Ly-49I a chain
Mouse Mouse Mouse Mouse Mouse Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer Buffer IgG1, k IgG2a, k IgG2b, k IgG3, k IgM, k
Ordering Information Description Format Size Cat. No.
BD Lyoplate Human Cell Surface Marker Screening Panel Contents: 242 human cell surface markers and 9 isotype controls Alexa Fluor® 647 5 tests 560747 (5 tests of each lyophilized purified mAb per well) Alexa Fluor® 647–conjugated goat anti-mouse Ig and goat anti-rat Ig detection reagents
BD Lyoplate Mouse Cell Surface Marker Screening Panel Contents: 176 mouse cell surface markers and 18 isotype controls (5 tests of each lyophilized purified mAb per well) Alexa Fluor® 647 5 tests 562208 Biotinylated goat anti-mouse, anti-rat, anti-Armenian hamster, and anti-Syrian hamster second steps Streptavidin Alexa Fluor® 647 detection reagent
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