IOTest® CD135-PE

PN IM2234U – 2 mL Liquid – 20 µL / test* – Clone SF1.340

Analyte Specific Reagent. Analytical and performance characteristics are not established.

SPECIFICITY REAGENT CONTENTS 2. Matthews, W., Jordan, C., Wiegand, G., The CD135 antigen, also known as FLT3 (1), This reagent is provided in phosphate- Pardoll, D., Lemischla, I., "A receptor Flk2 (2) or STK1 (3), is a member of the buffered saline, with 0.1% sodium azide tyrosine kinase specific to hematopoietic class III receptor tyrosine kinase (RTK) family (NaN3) as preservative, and 2.0 mg / mL stem and progenitor cell-enriched of transmembrane glycoproteins (4). bovine serum albumin (BSA). population", 1991, Cell, 65, 1143-1152. The CD135 antigen has a molecular weight STATEMENT OF WARNINGS 3. Small, D., Levenstein, M., Kin, E., of 130 – 155 kDa. Its extracellular region is 1. This reagent contains 0.1% sodium Carow, C., Amin, S., Rockwell, P., Witte, composed of five immunoglobulin like azide. Sodium azide under acid L., Burrow, C., Ratajczak, M., Gewirtz, domains. This receptor directly binds a conditions yields hydrazoic acid, an A., Civin, C., "STK-1, the human specific ligand (FLT3 ligand or FL), and homolog of FLK-2/FLT-3, is selectively extremely toxic compound. Azide + transduces regulatory signals through compounds should be flushed with expressed in CD34 human bone intracytoplasmic tyrosine kinase activity (4). running water while being discarded. marrow cells and is involved in the Other members of the class III RTKs are These precautions are recommended to proliferation of early progenitors / stem CD117 (or c-kit molecule), CD115 avoid deposits in metal piping in which cells", 1994, Proc. Natl. Acad. Sci. USA, (macrophage colony-stimulating factor explosive conditions can develop. If skin 91, 459-463. receptor: or M-CSFR), and CD140a and or eye contact occurs, wash excessively 4. Rosnet, O., Bühring, H.J., deLapeyrière, CD140b (or PDGFRs: two platelet-derived with water. O., Beslu, N., Lavagna, C., Marchetto., growth factor receptors) (4). 2. Do not use antibody beyond the S, Rappold, I., Drexler, H.G., Birg, F., The CD135 antigen is expressed on expiration date on the label. Rottapel, R., Hannum, C., Dubreuil, P., myelomonocytic and primitive B cell 3. Samples and all material coming in Birnbaum, D., "Expression and signal progenitors. On normal bone marrow cells, contact with them should be handled as transduction of the FLT3 tyrosine kinase expression of CD135 can be found on CD34- if capable of transmitting infection and receptor", 1996, Acta Haematol., 95, positive as well as CD34-negative cells. disposed of with proper precautions. 218-223. Most of the CD34bright, CD135-positive cells 4. Never pipet by mouth and avoid contact 5. Bühring, H.J., Birnbaum, D., Brasel, K., co-express CD117 at high levels. They may of samples with skin and mucous Civin, C.I., Götze, K., Lyman, S., represent early cycling, not quiescent stem membranes Rappold, I., Rosnet, O., "CD135 cells. CD135-positive cells in the CD34 low 5. Minimize exposure of reagent to light (FLT3 / FLK2 / STK1) workshop panel and CD34-negative populations do not co- during storage or incubation. report", 1997, Leucocyte Typing VI, express CD117 molecule and may represent 6. Avoid microbial contamination of White Cell Differentiation Antigens. B-lymphoid precursors. reagents or incorrect results might Kishimoto, T., et al, Eds., Garland The SF1.340 monoclonal antibody occur. Publishing, Inc., 875-879. recognizes an extracellular domain of 7. Use good laboratory practices when CD135. It has been assigned to the CD135 handling this reagent. PRODUCT AVAILABILITY cluster of differentiation during the 6th IOTest CD135-PE Conjugated Antibody International Workshop on Human Leucocyte STORAGE CONDITIONS AND STABILITY Differentiation Antigens, in Kobe, Japan, in This reagent is stable up to the expiration PN IM2234U – 2 mL Liquid – 20 µL / test*. 1996 (WS code: C-50 Section C) (5). date when stored at 2 – 8°C. Do not freeze. PE is licensed under patent 4,520,110. Minimize exposure to light.

REAGENT EVIDENCE OF DETERIORATION For additional information in the USA, call IOTest CD135-PE Conjugated Antibody Any change in the physical appearance of 800-526-7694. PN IM2234U – 2 mL Liquid – 20 µL / test*. this PE-labeled reagent (clear colorless to pinkish liquid) or any major variation in values Outside the USA, contact your local Clone SF1.340 obtained for control samples may indicate Beckman Coulter representative. Isotype IgG1, mouse deterioration and the reagent should not be Immunogen Transfected Ba/F3 cells used. www.beckmancoulter.com expressing FLT3 Hybridoma X63-Ag8.653 x Balb/c REAGENT PREPARATION Source Ascites fluid No preparation is necessary. This TRADEMARKS The Beckman Coulter logo and IOTest are Purification Ion exchange or affinity monoclonal antibody may be used directly chromatography from the vial. Bring reagent to 18 – 25°C prior trademarks of Beckman Coulter Inc. Conjugation R-phycoerythrin (PE) is to use. conjugated at 0.5 – 1.5 SELECTED RESEARCH REFERENCES Manufactured by: moles of PE per mole of Ig. 1. Rosnet, O., Marchetto, S., deLapeyrière, Immunotech, a Beckman Coulter Company Fluorescence PE (orange-red) O., Birnbaum, D., "Murine FLT3, a gene 130, avenue de Lattre de Tassigny, B.P. 177 Excites at 486 – 580 nm encoding a novel tyrosine kinase 13276 Marseille Cedex 9, France Emits at 568 – 590 nm receptor of the PDGFR/CSF1R family", 1991, Oncogene, 6, 1641-1650. Copyright© Beckman Coulter, Inc. 2006 All Rights Reserved

(*): 20 µL is the quantity of product sufficient to stain Page 1 of 1 I-IM2234U 2006-06-28 5 x 105 cells in a standard immunofluorescence assay