RESEARCH ARTICLE A Phylogeographic Assessment of the Malagasy Giant Chameleons (Furcifer verrucosus and Furcifer oustaleti) Antonia M. Florio*, Christopher J. Raxworthy Department of Herpetology, American Museum of Natural History, New York, NY, United States of America *
[email protected] a11111 Abstract The Malagasy giant chameleons (Furcifer oustaleti and Furcifer verrucosus) are sister spe- cies that are both broadly distributed in Madagascar, and also endemic to the island. These species are also morphologically similar and, because of this, have been frequently mis- identified in the field. Previous studies have suggested that cryptic species are nested within OPEN ACCESS this chameleon group, and two subspecies have been described in F. verrucosus.Inthis Citation: Florio AM, Raxworthy CJ (2016) A study, we utilized a phylogeographic approach to assess genetic diversification within these Phylogeographic Assessment of the Malagasy Giant chameleons. This was accomplished by (1) identifying clades within each species sup- Chameleons (Furcifer verrucosus and Furcifer oustaleti). PLoS ONE 11(6): e0154144. doi:10.1371/ ported by both mitochondrial and nuclear DNA, (2) assessing divergence times between journal.pone.0154144 clades, and (3) testing for niche divergence or conservatism. We found that both F. oustaleti Editor: Aristeidis Parmakelis, National & Kapodistrian and F. verrucosus could be readily identified based on genetic data, and within each spe- University of Athens, Faculty of Biology, GREECE cies, there are two well-supported clades. However, divergence times are not contemporary Received: June 16, 2015 and spatial patterns are not congruent. Diversification within F. verrucosus occurred during the Plio-Pleistocene, and there is evidence for niche divergence between a southwestern Accepted: April 8, 2016 and southeastern clade, in a region of Madagascar that shows no obvious landscape barri- Published: June 3, 2016 ers to dispersal.