Epigenetic Regulation of Cell Adhesion and Communication by Enhancer of Zeste Homolog 2 in Endothelial Cells Henryk Dreger, Antje Ludwig, Andrea Weller, Verena Stangl, Gert Baumann, Silke Meiners and Karl Stangl

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Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Original Article

Epigenetic Regulation of Cell Adhesion and Communication by Enhancer of Zeste Homolog 2 in Human Endothelial Cells Henryk Dreger, Antje Ludwig, Andrea Weller, Verena Stangl, Gert Baumann, Silke Meiners*, Karl Stangl*

Abstract—The histone methyltransferase enhancer of zeste homolog 2 (Ezh2) mediates trimethylation of lysine 27 in histone 3, which acts as a repressive epigenetic mark. Ezh2 is essential for maintaining pluripotency of stem cells, but information on its role in differentiated cells is sparse. Whole-genome mRNA expression arrays identified 964 that were regulated by >2-fold 72 hours after small interfering RNA-mediated silencing of Ezh2 in human umbilical vein endothelial cells. Among them, genes associated with the ontology terms cell communication and cell adhesion were significantly overrepresented, suggesting a functional role for Ezh2 in the regulation of angiogenesis. Indeed, adhesion, migration, and tube formation assays revealed significantly altered angiogenic properties of human umbilical vein endothelial cells after silencing of Ezh2. To identify direct target genes of Ezh2, we performed chromatin immunoprecipitation experiments followed by whole-genome arrays (chromatin immunoprecipitation-on-chip) and identified 5585 genes associated with trimethylation of lysine 27 in histone 3. Comparative analysis with our mRNA expression data identified 276 genes that met our criteria for putative Ezh2 target genes, upregulation by >2-fold after Ezh2 silencing and association with trimethylation of lysine 27 in histone 3. Notably, we observed a striking overrepresentation of genes involved in wingless-type mouse mammary tumor virus integration site (WNT) signaling pathways. Epigenetic regulation of several of these genes by Ezh2 was specifically confirmed by polymerase chain reaction analysis of DNA enrichment after chromatin immunoprecipitation using an antibody specific for trimethylation of lysine 27 in histone 3. Combining mRNA expression arrays and chromatin immunoprecipitation-on-chip analysis, we identified 276 Ezh2 target genes in endothelial cells. Ezh2-dependent repression of genes involved in cell adhesion and communication contributes to the regulation of angiogenesis. (Hypertension. 2012;60:00-00.) ● Online Data Supplement Key Words: enhancer of zeste homolog 2 ◼ epigenetics ◼ angiogenesis ◼ histone methylation ◼ cell communication ◼ cell adhesion ◼ wingless-type MMTV-integration site signaling

pigenetic modifications, such as DNA and histone meth- thereby relieving repression of PRC target genes and thus Eylation, have long-term effects on . contributing to target gene activation.3,4 In tissue-specific The histone methyltransferase enhancer of zeste homolog ­differentiation studies, Ezh2 was shown to play a crucial role 2 (Ezh2) mediates trimethylation of lysine 27 of histone 3 in heart development, neurogenesis, skeletal muscle differ- (H3K27me3), which acts as a repressive epigenetic mark.1 entiation, and adipogenesis, as well as in the generation of Ezh2 belongs to the Polycomb group that are epidermal progenitor cells.4–8 well-characterized regulators of the epigenome. These pro- In addition to stem cells, cells have been shown to teins were first identified in Drosophila, where they form express high levels of Ezh2, which is associated with poor Polycomb repressive complexes (PRC) and suppress homeo- outcome.9,10 Among other mechanisms, this has been attributed tic genes responsible for segment identity.2 PRCs are highly to Ezh2-mediated silencing of vasoinhibin-1 and subsequent conserved also in and have been shown to be essen- promotion of angiogenesis.10 In contrast, little is known tial for maintaining pluripotency by repression of develop- about Ezh2 target genes in differentiated cells. Interestingly, mental genes in human stem cells.2,3 During differentiation, transcriptomic studies on cells and tissues suggest a relatively expression of PRC subunits, among them Ezh2, decreases, high expression of Ezh2 in endothelial cells.11 Furthermore,

Received January 9, 2012; first decision February 3, 2012; revision accepted August 8, 2012. From the Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte, Charité – Universitätsmedizin Berlin, Berlin, Germany (H.D., A.L., A.W., V.S., G.B., K.S.); and Comprehensive Pneumology Center, Ludwig-Maximilians-Universität, Asklepios Fachkliniken München-Gauting and Helmholtz Zentrum München, München, Germany (S.M.). *These authors contributed equally to this work. The online-only Data Supplement is available with this article at http://hyper.ahajournals.org/lookup/suppl/doi: 10.1161/HYPERTENSIONAHA. 112.191098/-/DC1. Correspondence to Henryk Dreger, Medizinische Klinik für Kardiologie und Angiologie, Charité – Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany. E-mail [email protected] © 2012 American Heart Association, Inc. Hypertension is available at http://hyper.ahajournals.org DOI:10.1161/HYPERTENSIONAHA.112.191098 Downloaded from http://hyper.ahajournals.org/1 by guest on September 11, 2012 2 Hypertension November 2012 a recent study demonstrated that the migratory properties Tube Formation Assays of endothelial cells are altered by microRNA-mediated Seventy-two hours after siRNA transfection, HUVECs were trans- inhibition of Ezh2.12 These data indicate a role for Ezh2- ferred to 24-well plates that had been prepared with 230 µL of matri- dependent epigenetic control of endothelial cell function gel per well (Basement Membrane Matrix Phenol Red Free; BD in vessel formation and angiogenesis. In addition, studies Biosciences, Heidelberg, Germany). After 23 hours, 5 digital pictures were taken per well, and tube formation was subsequently analyzed on the Hutchinson–Gilford progeria syndrome suggest that in a blinded fashion using a commercial imaging service (Wimasis accelerated atherosclerosis—a hallmark of this childhood GmbH, Munich, Germany). disease—is caused, at least in part, by a loss of epigenetic control, including a deficit of repressive epigenetic marks Migration Assay such as H3K27me3.13 To assess cell migration, we performed scratch assays. The cell-free Taken together, there is growing evidence for a physiologi- area was digitally quantified (Photoshop; Adobe Systems, Inc) imme- cal role of Ezh2 in differentiated endothelial cells. The aim of diately, as well as 16 hours after scraping the cell monolayer with a 17 our present study was to further elucidate the function of Ezh2 pipette tip. by identification of its target genes in endothelial cells. Adhesion Assay Methods After blocking with 0.5% BSA (Sigma) in PBS, 5×104 cells were incubated in fibronectin-coated 24-well plates (BD Biosciences) Detailed information on materials and methods are provided in the for 1 hour at 37°C. Subsequently, plates were agitated at 400 revo- online-only Data Supplement. lutions per minute for 15 seconds. After washing, adherent cells were fixed with paraformaldehyde and stained using 0.1% crystal Cell Culture violet (Sigma). Finally, absorption was quantified after adding 10% Human umbilical vein endothelial cells (HUVECs) from different acetic acid at 570 nm. donors were prepared and kept in culture, as described previously.14 The cell preparation conformed to local university ethics guidelines Chromatin Immunoprecipitation and the principles outlined in the Declaration of Helsinki. Chromatin immunoprecipitation (ChIP) was performed accord- 18 Small Interfering RNA Transfections and mRNA ing to a protocol published by Dahl et al, with minor modifica- tions using an H3K27me3 antibody (ab6002; Abcam, Cambridge, Expression Analysis United Kingdom) or an unspecific mouse IgG (sc2025; Santa Cruz, Cells were transfected with 25 nmol/L of control small interfer- Heidelberg, Germany). Purified DNA was either used for ChIP-PCR ing RNA (siRNA) (Silencer Select Negative Control Ambion, experiments or further amplified using a whole-genome amplifica- Austin, TX) or siRNA directed against Ezh2 (s4918; Ambion) tion kit (WGA2; Sigma) for ChIP-on-chip experiments. using Oligofectamine (Invitrogen). Seventy-two hours after trans- fection, total mRNA was harvested, as described previously.14 For quantitative reverse transcriptase PCR analysis, RNA was reverse Promoter Chip Arrays (ChIP-on-Chip) transcribed and amplified using the TaqMan method, as published DNA enrichment by ChIP using the H3K27me3-specific anti- previously.15 Primer details are given in Table S1 in the online- body was compared with the input control in whole-genome pro- only Data Supplement. For mRNA expression arrays (Agilent moter chip arrays (NimbleGen 2.1M Deluxe Promoter; imaGenes, Whole Microarray, 4 × 44K; Agilent, Böblingen, Berlin, Germany). H3K27me3 marks were detected using the data Germany), DNase-digested RNA from 3 independent experiments from 3 independent experiments (NimbleScan 2.3; NimbleGen, was purified, as described recently.16 Waldkraiburg, Germany).

Table 1. Pathway Overrepresentation Among Ezh2 Target Quantification of DNA Enrichment by Genes Based on PANTHER Gene Annotation Analysis ChIP (ChIP-PCR) DNA enrichment by ChIP was analyzed by quantitative amplification Genes on using the SYBR Green method and specific primers (Table S1) that Homo Sapiens Ezh2 were designed to match the regions identified previously as associ- Reference Target ated with H3K27me3 in ChIP-on-chip experiments. DNA enrichment Pathway List (n) Genes (n) P Value with the H3K27me3 antibody was compared with results obtained WNT signaling pathway 317 15 0.00002 using an unspecific IgG control (sc2025; Santa Cruz). Alzheimer disease-presenilin pathway 122 9 0.00004 Bioinformatic Analysis and Statistics Cadherin signaling pathway 147 9 0.0002 Array data were analyzed using the software Ontologizer v2.1 Integrin signaling pathway 181 9 0.0007 (Computational Biology Group, Institute for Medical Genetics and Angiogenesis 191 9 0.001 Human Genetics, Charité – Universitätsmedizin Berlin, Berlin, Germany; http://compbio.charite.de/index.php/ontologizer2.html). Inflammation mediated by chemokine 283 11 0.001 Significantly overrepresented (GO) terms were identi- and cytokine signaling pathway fied by parent-child analysis and Bonferroni correction for multiple α-Adrenergic signaling pathway 32 3 0.009 testing. Enrichment of single biological pathways compared with a Cell cycle 22 2 0.033 human reference list was analyzed using the analysis through evolutionary relationships classification software (http://www. Endogenous cannabinoid signalling 24 2 0.039 pantherdb.org). Pathways (consisting of >1 gene) were considered Ezh2 indicates enhancer of zeste homolog 2; PANTHER, protein analysis through ­significantly overrepresented whenP values were <0.05. evolutionary relationships; WNT, wingless-type mouse mammary tumor virus Statistical significance of siRNA-mediated knockdown of Ezh2 integration site. was calculated using Student t test (SigmaStat 3.0; SPSS, Inc). An Details are given in Table S5. error P<0.01 was regarded as significant. Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Dreger et al Ezh2 Target Genes in Endothelial Cells 3

Results siRNA-mediated knockdown of Ezh2 using 3 different methods: adhesion assays revealed significantly increased Identification of Genes Regulated by Knockdown adhesion of Ezh2-silenced HUVECs to fibronectin (Figure of Ezh2 1A). Scratch assays showed enhanced migration of endothe- H3K27me3 represents a covalent and thus lasting histone modi- lial cells after Ezh2 knockdown (Figure 1B). Furthermore, fication. Importantly, however, Ezh2 is essential to propagate silencing of Ezh2 reduced the number of tubes formed in 19 H3K27me3 to the next cell generation during proliferation. matrigel assays compared with cells transfected with con- Furthermore, H3K27me3 can be removed by histone demeth- trol siRNA, indicating altered cell–cell communication ylases, such as Jmjd3 or ubiquitously transcribed tetratricopep- (Figure 1C). tide repeat X (UTX).20 We, therefore, speculated that, over time, loss of Ezh2 would lead to a detectable (re-)expression of its Identification of Genes Associated With H3K27me3 target genes. To obtain information on Ezh2-regulated genes, by ChIP-on-Chip Experiments we, therefore, performed differential whole-genome expression To analyze which of the genes whose expression changed arrays after siRNA-mediated knockdown of Ezh2. In a first step, after knockdown of Ezh2 are direct targets of the histone we confirmed efficient knockdown of Ezh2 in HUVECs 72 methyltransferase, we next performed ChIP experiments fol- hours after siRNA transfection: Ezh2 expression was reduced lowed by whole-genome promoter arrays (ChIP-on-chip). by 64% on the mRNA level and virtually absent on the protein For that, we used an antibody that specifically detects the level as shown by quantitative reverse transcriptase PCR and epigenetic mark mediated by Ezh2. Bioinformatic analysis immunoblots, respectively (Figure S1 in the online-only Data of DNA enrichment by the H3K27me3 antibody compared Supplement). Silencing of Ezh2 had no significant effect on cell with input control identified 5585 genes that contained≥ 1 morphology, viability, or proliferation rate (Figure S2). H3K27me3 marks in their promoter region (Table S3). In Analysis of mRNA expression arrays identified 964 genes a second step, we performed a comparative analysis of our that were regulated by >2-fold 72 hours after knockdown of ChIP-on-chip and mRNA expression array data. Because Ezh2 (Table S2). Bioinformatic analysis revealed that genes Ezh2-mediated trimethylation of lysine 27 in histone 3 associated with the GO terms cell adhesion (GO:0007155), results in gene silencing, we defined putative Ezh2 target biological adhesion (GO:0022610), and cell communication genes as H3K27me3 positive and upregulated by >2-fold (GO:0007154) were significantly overrepresented (P<0.01) after Ezh2 knockdown. Using this comparative approach, among genes regulated by Ezh2 knockdown (Figures S3 we identified 276 putative Ezh2 target genes in endothelial and S4). cells (Table S4).

Silencing of Ezh2 Alters Angiogenic Activity of Ezh2-Mediated Regulation of Cell Adhesion and Endothelial Cells Communication Because our GO term analysis suggested a functional To obtain an unbiased view on the pattern of Ezh2 tar- role for Ezh2 in the regulation of angiogenesis, we ana- get genes, we first performed a GO term analysis. Again, lyzed the angiogenic potential of HUVECs 72 hours after genes associated with the GO terms cell adhesion and cell

2.0 1.5 A P<0.05 B P<0.05 n=4 n=3 1.5 1.0

1.0

0.5

0.5 Regrown area Relative adhesion [arbitrary units ± SD] [arbitrary units ± SD]

0.0 0.0 Figure 1. Adhesion of endothelial cells on Control Ezh2 Control Ezh2 fibronectin-coated plates A( ), regrown area in scratch assays (B), and tube formation in matrigel C assays (C) after small interfering RNA (siRNA)- 160 P<0.05 mediated knockdown of enhancer of zeste homolog 140 n=3 2 (Ezh2).

120 Control 100

80

60 Tubes per field

40 Ezh2 0 Control siRNA Ezh2 siRNA

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celladhesion cellcommunication other (GO:0007155) (GO:0007154) geneID fold change gene ID fold change gene ID fold change gene ID fold change gene ID fold change gene ID fold change ADAM12 2.2 ADRA1B 2.2 ACTA1 2.3 CPM 3.3 KIAA0644 2.1 RTN1 2.3 ANTXR1 2.6 ADRA2A 2.2 ACTG2 2.6 CRLF1 2.8 KIAA1217 2.3 SEMA6A 2.2 CDH13 2.5 ADRA2C 2.0 ADAMTS12 4.2 CYP2J2 2.4 KIF1A 2.3 SERPINC1 3.2 CNTN1 2.3 APC2 2.2 ADAMTS15 2.1 CYTL1 2.0 KIF5C 4.1 SEZ6 2.2 COL12A1 2.4 BAIAP3 2.7 AFF2 3.4 DACT1 3.1 KLF17 2.6 SEZ6L2 2.1 COL21A1 3.0 BDNF 2.2 ALDH1L1 2.9 DACT3 2.4 LAIR1 2.6 SH2D4A 2.3 COL6A2 2.1 BMPR1A 2.6 ANKRD19 2.5 DENND2A 4.2 LBH 2.2 SHANK1 2.2 CPXM1 2.1 BSN 2.3 ANKRD7 2.1 DFNB31 2.0 LOR 2.6 SLC10A4 4.1 EPDR1 3.1 C1orf187 4.6 APCDD1L 2.5 DHRS2 2.2 LPL 6.8 SLC16A9 2.6 FGF1 5.6 CCL26 2.1 ARNT2 2.7 DIRAS1 2.0 MAF 2.0 SLC22A15 2.2 FZD7 3.2 CDH13 2.5 ATP2A3 2.5 DSCR6 2.4 MAGEL2 2.7 SLC26A10 3.0 HAPLN4 2.3 CNR1 2.2 ATP2C2 2.0 DTWD2 2.1 MMP24 2.3 SLC26A9 2.1 IL1B 2.0 CXXC4 4.3 AUTS2 2.5 DUSP26 2.6 MOBKL2B 2.0 SLC2A9 2.1 ITGA11 2.5 CYP26A1 2.4 B3GAT1 6.8 ENST00000323355 6.0 MORN5 2.3 SLC4A3 2.7 ITGA4 5.8 EFNB3 2.4 BC006530 2.5 EPHA5 3.2 MPO 2.0 SLC6A13 2.6 ITGA8 2.2 EGR2 2.3 BCL11A 4.7 EPHB3 2.6 MYCN 2.4 SLC7A10 2.9 ITGA9 2.4 EPHB1 2.2 BCL11B 2.2 FAM155B 3.0 AP1L3 3.0 SMOC1 3.7 KAL1 2.5 FGF1 5.6 BEAN 4.0 FAM19A5 3.1 NECAB2 2.1 SNIP 2.2 LEF1 2.7 FGF131 5.6 BEND5 2.5 FAM43B 2.9 NEFM 4.5 SNTB1 4.0 MEGF10 2.9 FZD7 3.2 BEX2 2.6 FBLN1 2.6 NHS 2.8 SORL1 2.3 MUC4 3.3 GABRB3 2.4 BTBD11 2.1 FBXL16 3.4 NKAIN1 2.9 SOX11 7.9 NCAM1 2.1 GAL3ST1 2.7 C10orf35 3.2 FLJ30901 2.7 NPAS3 3.2 SRrp35 7.0 PCDHB2 5.1 GJC3 3.3 C12orf42 4.0 FMN2 2.1 NPFFR2 4.1 ST8SIA5 3.3 PDZD2 2.2 HEY2 4.0 C18orf1 3.0 FXYD4 2.1 NPPB 3.8 STOX2 2.0 SPON1 2.0 IGF2 2.6 C1QTNF1 2.4 FZD3 2.2 NTRK1 2.3 THNSL2 2.8 SPON2 4.0 IGFBP3 2.9 C20orf112 2.4 GALNT14 2.7 NUDT10 2.1 TMEM108 5.8 TESC 2.1 IGFBP5 6.6 C20orf46 2.0 GATM 2.4 OGDHL 2.1 TMEM176B 2.2 TGFBI 2.7 IL11 3.1 C20orf85 2.6 GGT7 2.2 PADI2 2.2 TMEM200A 4.4 VAV3 2.4 IL1B 2.0 C3orf70 2.9 GLT8D2 2.0 PARD6G 2.6 TMEM45A 2.5 VCAN 4.3 IRS1 3.4 C6orf168 3.0 GNG2 2.8 PCOLCE2 2.3 TMEM92 2.0 ITGA8 2.2 C8orf47 2.3 GRID1 2.4PCSK6 2.6 TSHZ2 2.2 KCNMA1 2.2 C9orf125 2.4 GRPR 2.0PDE9A 2.6 TUBB2B 4.4 KIT 2.6 CACNA2D4 2.5 GUCY1A2 2.4PHF21B 3.0 UNC5C 2.0 5-fold KREMEN1 2.2 CBLN2 2.0 GYG2 2.3 PIK3AP1 2.1 UST 3.9 LEF1 2.7 CCDC102B 2.0 HEYL 2.1 PLXDC2 2.5 UTP23 2.5 NCAM1 2.1 CCDC8 2.1 HS3ST3B1 3.1 PPARG 3.4 WNK4 2.3 NOS2 3.9 CCND2 2.4 HS6ST3 2.1 PRG2 3.4 XYLT1 2.9 NPTX2 3.6 CDC14B 2.4 IGSF3 2.0 PRMT8 2.3 ZCCHC12 2.2 PCDHB2 5.1 CEACAM21 2.6 IL10RA 2.3 PROM1 2.3 ZFP37 2.1 PCSK1 2.3 CECR6 2.5 IL17RD 2.1 PTN 7.5 ZMAT4 2.6 PPFIA4 2.4 CH25H 3.3 IRX2 2.0 RAB11FIP4 2.7 ZNF167 2.2 SLC8A2 2.1 CHGB 2.2 ITM2C 4.2 RAI2 2.4 ZNF365 2.1 SNAI2 3.5 CMKLR1 2.8 JPH3 4.5 RASGRF2 3.0 STC1 2.1 CNIH3 2.6 KALRN 2.6 RASGRP1 2.2 SYN2 2.2 CNN1 3.7 KBTBD11 3.2 RASGRP2 2.0 2-fold TGFA 3.4 COCH 4.2 KCNB2 2.7 RCAN2 2.3 TMOD2 2.3 COL25A1 2.0 KCNF1 2.0 RCSD1 2.8 TPD52L1 2.5 COL4A1 2.5 KCNK12 2.2 RIMBP3 4.3 WNT5B 2.8 CPAMD8 2.0 KCNN2 2.2 RNF182 2.5 Figure 2. Heat maps of H3K27me3-associated genes that were upregulated by >2-fold after enhancer of zeste homolog 2 (Ezh2) knockdown (n=3). communication were significantly overrepresented among genes are involved in >1 pathway, this analysis enclosed 39 Ezh2-regulated genes (Figure 2). Next, we functionally anno- different Ezh2 target genes (Table S5). We selected 7 newly tated putative Ezh2 target genes using the protein analysis identified Ezh2 target genes that are involved in cell adhe- through evolutionary relationships ontology and gene expres- sion and communication: cadherin 13, integrin α4, fibro- sion tool. This software allows identification of genes and growth factor 1, transforming growth factor-α, pathways that are overrepresented compared with a human homolog 7, lymphoid enhancer–binding factor 1, and WNT reference list.21 As depicted in Table 1, analysis of cellular family member 5B (WNT5B). For all 7 genes, ChIP-PCR pathways revealed a striking overrepresentation of genes experiments showed significant DNA enrichment by ChIP involved in angiogenesis and in the wingless-type mouse using the H3K27me3 antibody compared with the unspecific mammary tumor virus integration site (WNT), cadherin, IgG control. Importantly, siRNA-mediated knockdown of and integrin signaling pathways among Ezh2 target genes. Ezh2 significantly reduced DNA enrichment, indicating a In total, 9 pathways were overrepresented. Because several loss of H3K27 trimethylation marks at these promoter sites

Table 2. Selected Ezh2 Target Genes Implicated in Vasculogenesis and Angiogenesis

Gene ID Name Function ADAM12 A disintegrin and metalloproteinase domain 12 Promotion of cell spreading26 CDH13 Cadherin 13 Regulation of vascular cell adhesion, phenotype, and motility25 FGF1 Fibroblast growth factor 1 Induction of cell cycle genes and migration in endothelial cells24 FZD7 Frizzled homolog 7 Regulation of endothelial cell spreading27 HEY2 Hairy/enhancer-of-split related with YRPW motif 2 Vasculogenesis29 TGFA Transforming growth factor-α Angiogenesis28 VCAN Versican Required for proliferation and migration of vascular smooth muscle cells30 Ezh2 indicates enhancer of zeste homolog 2. Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Dreger et al Ezh2 Target Genes in Endothelial Cells 5

Table 3. Selected Ezh2 Target Genes Implicated in Endothelial Dysfunction

Gene ID Name Function BMPR1A Bone morphogenetic protein receptor 1A Downregulated in pulmonary arterial hypertension34 IL1B Interleukin 1β Induction of intercellular adhesion molecule 1 and vascular adhesion molecule 1 in endothelial cells; monocyte adhesion31,32 SPON1 Spondin 1 Hypertension candidate gene35 IGF2 Insulin-like growth factor 2 Regulation of hemangioma growth33 IL11 Interleukin 11 Induction of resistance against immune-mediated injury in endothelial cells36 IRS1 Insulin receptor substrate 1 Required for insulin-dependent NO production in endothelial cells40 NOS2 Inducible NO synthase Production of NO37 TGFBI Transforming growth factor-β–induced Overexpressed in atherosclerotic lesions; regulation of adhesion and migration of gene vascular smooth muscle cells in atherogenesis38,39 Ezh2 indicates enhancer of zeste homolog 2; NO indicates nitric oxide. on Ezh2 silencing (Figure 3A–3G). Furthermore, reduced using a second siRNA targeting a different sequence of Ezh2 Ezh2-mediated methylation of these promoters was associ- (not shown). ated with significantly enhanced mRNA expression of all 7 genes as revealed by quantitative reverse transcriptase- Discussion PCR analysis (Figure 3H). The effect of Ezh2 silencing on Ezh2 is a well-characterized regulator of the epigenome the expression of the selected target genes was confirmed and has been shown to be instrumental for maintaining

A 150 B Control siRNA CDH13 150 Control siRNA ITGA4 * * Ezh2 siRNA Ezh2 siRNA 100 100

50 50 # # DNA enrichment DNA enrichment [arbitrary units ± SEM] [arbitrary units ± SEM] 0 0 IgG H3K27me3 IgG H3K27me3 C D 150 FGF1 150 Control siRNA TGFα Control siRNA * * Ezh2 siRNA Ezh2 siRNA 100 100 Figure 3. A to G, DNA enrichment by 50 50 chromatin immunoprecipitation using the # # specific H3K27me3 antibody compared with DNA enrichment DNA enrichment the unspecific control IgG 72 hours after

0 [arbitrary units ± SEM] 0 [arbitrary units ± SEM] IgG H3K27me3 IgG H3K27me3 small interfering RNA (siRNA)-mediated enhancer of zeste homolog 2 (Ezh2) E 150 Control siRNA F 150 Control siRNA knockdown (*P<0.05 vs IgG+control * FZD7 * LEF1 siRNA, #P<0.05 vs anti-H3K27me3+control Ezh2 siRNA Ezh2 siRNA siRNA). H, Relative mRNA expression 100 100 72 hours after transfection of siRNA directed against Ezh2 compared with cells transfected with control siRNA (P<0.05 for 50 50 all genes vs control siRNA). # # DNA enrichment DNA enrichment [arbitrary units ± SEM] [arbitrary units ± SEM] 0 0 IgG H3K27me3 IgG H3K27me3

G 150 H 12 Control siRNA WNT5B 10 Ezh2 siRNA * 100 8 6 50 4 # 2 DNA enrichment mRNA expression [arbitrary units ± SEM] 0 [arbitrary units ± SEM] 0 3 IgG H3K27me3 F1 5B H1 G EF1 T FZD7 L ITGA4 F TGFA N CD W

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 6 Hypertension November 2012 pluripotency in human stem cells.2,3 In contrast, little is known Ezh2 tar­ get genes, such as fibroblast growth factor 1, cadherin about its function in differentiated cells. Recent studies, how- 13, a disintegrin and metalloproteinase domain 12, frizzled ever, have suggested a physiological role for Ezh2 in endothe- homolog 7, transforming growth factor-α, hairy/enhancer- lial function.12,22 Therefore, the aim of our present study was of-split related with YRPW motif 2, and versican are known to identify functionally relevant target genes of Ezh2 in human to play a role in the regulation of vascular cell proliferation, endothelial cells. spreading, and angiogenesis (Table 2).24–30 Furthermore, some To assess Ezh2-regulated gene expression, we ana- other Ezh2 tar­ get genes have been implicated in endothelial lyzed the impact of Ezh2 knockdown on mRNA expression dysfunction and cardiovascular disease (eg, interleukin 1β, in endothelial cells and found 946 genes to be regulated insulin-like growth factor 2, bone morphogenetic protein by >2-fold. Almost two thirds of the genes were upregulated receptor 1A, spondin 1, interleukin 11, inducible nitric oxide after Ezh2 knockdown, which accords with the reported synthase, transforming growth factor-β–induced gene, and 1 repressive function of Ezh2. In a study using embryonic insulin receptor substrate (Table 3).31–40 23 fibroblasts, Bracken et al reported on 341 significantly reg- Interestingly, functional annotation of all Ezh2 target ulated genes 44 hours after siRNA-mediated knockdown of genes revealed a striking enrichment of genes involved in the several PRC2 subunits. The longer incubation time used in our WNT signaling pathway. Numerous genes from this path- study (72 versus 44 hours) may account for the larger number way are also associated with other enriched pathways, such of affected genes. Because little is known about the function as angiogenesis, Alzheimer disease-presenilin, and cadherin of Ezh2 in endothelial cells, we sought to identify a functional signaling pathways (Table S5). Several of these overlapping pattern among the genes regulated by silencing of Ezh2. In genes, namely cadherin 13, frizzled homolog 7, WNT5B, our unbiased bioinformatic analysis, we included all genes and lymphoid enhancer–binding factor 1, as well as fibro- up- or downregulated by >2-fold after Ezh2 knockdown, irre- blast growth factor 1, transforming growth factor-α, and spective of their association with H3K27me3. This approach integrin 4, were shown to be directly regulated by Ezh2: gives a complete picture of the role of Ezh2 in endothelial α cells because it also includes genes that might be indirectly first, DNA enrichment by ChIP using the H3K27me3 anti- regulated by Ezh2. The emerging pattern clearly indicates body confirmed an association of the promoter regions of all that endothelial cells respond to Ezh2 silencing with altered selected genes with the epigenetic mark mediated by Ezh2. cell–cell communication and cell adhesion. This particular Importantly, DNA enrichment was significantly attenuated gene expression profile is well in accordance with the altered in Ezh2-deficient cells (Figure 3A–3G). Second, siRNA- angiogenic properties of endothelial cells observed in adhe- mediated knockdown of Ezh2 significantly induced mRNA sion, scratch, and tube formation assays after Ezh2 silencing levels of all 7 genes as determined by quantitative reverse (Figure 1).12,22 transcriptase-PCR (Figure 3H). These data provide first evi- To identify genes carrying the Ezh2-mediated epigenetic dence that the reported involvement of the WNT pathway mark H3K27me3, we performed ChIP-on-chip experiments in vasculogenesis and angiogenesis may be regulated on an and detected 5585 genes for which the promoters were associ- epigenetic level by trimethylation of K27 in histone 3 via ated with H3K27me3 (Table S3). Using a similar approach, Ezh2.41,42 This is well in line with recent reports that revealed Lee et al3 identified 1007 H3K27me3-positive promoters Ezh2-mediated regulation of the WNT pathway in adipogen- in human embryonic stem cells. Interestingly, 69% of the esis and liver carcinogenesis.7,43 Our results, however, also H3K27me3-positive genes reported by Lee et al3 were also add complexity because WNT5B represents a ligand for associated with H3K27me3 in our study, indicating a sur- noncanonical WNT signaling, which may counteract canoni- prisingly large concordance between human embryonic stem cal WNT signaling.44 and endothelial cells. In addition, an earlier study conducted on human embryonic fibroblasts reported 2206 H3K27me3- Perspective positive promoters.23 Thus, our number is somewhat higher Taken together, our data indicate that Ezh2 is not only but still within the range of earlier reports. involved in the regulation of endothelial cell proliferation, Having identified genes that contain an H3K27 trimeth- spreading, and motility in angiogenesis but may also play a ylation mark and genes regulated on the mRNA level by role for endothelial function in response to inflammation and silencing of Ezh2 in endothelial cells, we next combined the growth factor signaling. Thus, our study expands the current results of our ChIP-on-chip and Ezh2 knockdown expres- knowledge on Ezh2 target genes from stem to differentiated sion arrays to single out direct Ezh2 target genes. We defined cells and may prompt additional research characterizing the putative Ezh2 target genes as H3K27me3 positive, as well as function of Ezh2 in endothelial dysfunction and cardiovascu- upregulated by >2-fold after Ezh2 knockdown. This algorithm lar disease. yielded 276 target genes, with predominant overrepresenta- tion of genes associated with the GO terms cell adhesion and Sources of Funding cell communication (Figure 2). Several of these Ezh2 target The study was supported by a research grant from the Deutsche genes are involved in the WNT and fibroblast growth factor Forschungsgemeinschaft to K. Stangl (STA 567/4-1). signaling pathways, which have been shown previously to be regulated by Ezh2 in human embryonic stem cells and fibro- Disclosures blasts.3,23 More importantly, a variety of our newly identified None.

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Novelty and Significance

What Is New? • Epigenetic (dys-)regulation of endothelial function may be an important co- • First report on the target genes of the histone methyltransferase, enhancer factor in the pathogenesis of cardiovascular disease. of zeste homolog 2 (Ezh2), in endothelial cells. Summary • First report on a possible role for an epigenetic regulation of genes involved in cell communication, adhesion, and angiogenesis by Ezh2 in endothelial Combining mRNA expression arrays and chromatin immunoprecipi- cells. tation-on-chip analysis after small interfering RNA–mediated knock- down of Ezh2, we identified 276 Ezh2 target genes in endothelial What Is Relevant? cells. Ezh2-dependent repression of genes involved in cell adhesion • Endothelial dysfunction plays an important role in cardiovascular disease. and communication contributes to regulation of angiogenesis.

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Online supplement

Epigenetic regulation of cell adhesion and communication by Enhancer of zeste homolog 2 in human endothelial cells

Henryk Dreger1, Antje Ludwig1, Andrea Weller1, Verena Stangl1, Gert Baumann1, Silke Meiners2, and Karl Stangl1

1) Medizinische Klinik für Kardiologie und Angiologie, Campus Mitte Charité – Universitätsmedizin Berlin, Germany 2) Comprehensive Pneumology Center (CPC) Ludwig-Maximilians-Universität, Asklepios Fachkliniken München-Gauting and Helmholtz Zentrum München

Correspondence to: Henryk Dreger, MD Medizinische Klinik für Kardiologie und Angiologie Charité – Universitätsmedizin Berlin Charitéplatz 1 D – 10117 Berlin, Germany Phone: +49 30 450 613 496 Fax: +49 30 450 513 941 Email: [email protected]

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Expanded Materials and Methods

Cell culture HUVEC were isolated by collagenase type II (Biochrom KG, Berlin, Germany) digestion of human umbilical veins and cultured at 37°C with 5% CO2 in EC medium (MCDB 131, Gibco BRL, Life Technologies GmbH, Karlsruhe, Germany) with the following supplements: 20 µg/mL Endothelial Cell Growth Supplement (PromoCell, Heidelberg, Germany), 1 ng/mL fibroblast growth factor  (Biomol, Hamburg, Germany), 0.1 ng/mL endothelial growth factor (Biomol), 2% fetal calf serum (Biochrom), 2 mmol/L L-glutamine (Gibco), 1 µg/mL hydrocortisone (Sigma-Aldrich, Hamburg, Germany), 1 IU/mL heparin (Biochrom), 5 IU/mL penicillin and 5 µg/mL streptomycin (Biochrom).

Western blot analysis In short, equal amounts of proteins were separated by SDS-polyacrylamide gel electrophoresis and membranes were incubated with antibodies against Ezh2 (#3147, Cell Signaling, Danvers, MA) and -actin as a loading control (Cell Signaling). Bands were detected using ECL plus (GE Healthcare, Munich, Germany). Densitometric analyzes were performed using ImageJ (U.S. National Institutes of Health, http://rsb.info.nih.gov/ij/).

Chromatin immunoprecipitation (ChIP) HUVEC were cultured in 75 cm2 flasks (BD Falcon, Heidelberg, Germany) until they reached 80% confluence. Cells were trypsinized, rinsed and resuspended in phosphate buffered saline (PBS) with 1 mmol/L phenylmethylsulfonyl fluoride (PMSF) as a serine proteinase inhibitor and completeTM (Roche, Grenzach-Wyhlen, Germany) as a proteinase inhibitor. DNA and chromatin were cross-linked by adding formaldehyde to a final concentration of 1% (v/v) at room temperature under constant rotation for ten minutes. To stop cross-linking, cells were incubated in the presence of 125 mmol/L glycine at room temperature for 5 minutes. After two washing steps using ice cold PBS with PMSF and completeTM, cells were centrifuged and lysed at room temperature in 200 µL lysis buffer (40 mmol/L Tris pH 8.0, 10 mmol/L EDTA, 1% SDS, 20 mmol/L sodium butyrate, 1 mmol/L PMSF plus completeTM). To obtain DNA fragments of about 500 bp length, lysates were sonicated using a Sonoplus HD70 (Bandelin, Berlin, Germany). After centrifugation at 10,000 g for ten minutes, supernatants were transferred into fresh tubes, diluted in 1.8 mL RIPA buffer (50 mmol/L Tris pH 7.5, 150 mmol/L NaCl, 1% NP40, 0.5% sodiumdeoxycholate, 0.1% SDS) and incubated with 25 µL protein A and 25 µL protein G magnetic beads (Invitrogen, Darmstadt, Germany) with constant rotation at 4°C for three hours. At this point, 10% of the DNA chromatin mixture was saved for later use as input control. The remainder was added to 25 µL pre-washed beads and incubated over night at 4°C with 5 µg of an antibody specifically directed against H3K27me3 (ab6002, Abcam, Cambridge, UK) or an unspecific mouse IgG (sc2025, Santa Cruz, Heidelberg, Germany). After three washing steps with ice cold RIPA buffer and three washing steps with TE buffer (10 mmol/L Tris pH 8.0, 1 mmol/L EDTA) the beads were captured using a magnetic rack, resuspended in elution buffer (20 mmol/L Tris pH 7.5, 5 mmol/L EDTA, 50 mmol/L NaCl, 1% SDS plus 50 µg/mL proteinase K) and incubated at 68°C with constant shaking at 1300 rpm for three hours. DNA was extracted with phenol-chloroform isoamylalcohol and purified using a PCR Purification Kit (Qiagen, Hilden, Germany).

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Table S1: Primer information

1. Primers for mRNA expression analysis All RNA primers were purchased from Applied Biosystems, Carlsbad, CA. Expression of mRNA was standardized to the housekeeping gene RPL19 and normalized to expression after transfection of control siRNA using the comparative Ct method (2-ct).

Gene Name Primer ID CDH13 Hs00169908_m1 EZH2 Hs00172783_m1 FGF1 Hs00265254_m1 FZD7 Hs00275833_s1 ITGA4 Hs00168433_m1 LEF1 Hs01547250_m1 TGFA Hs00608187_m1 WNT5B Hs01086864_m1 RPL19 Hs02338565_gH

2. Primers for DNA quantification by ChIP-PCR Gene Name Forward Reverse CDH13 TTCACTTTCCCTTTGTTCTAGCTCA CAGCCCTCCTGTCCCAATC FGF1 TGCAAACTCACAGGATTATCATGAC TCAAGGTGCTCGAACACACTTG FZD7 GAAACTGATGCTTGGGAGGAAGA TTGGGCCAAGGCAGAGTGT ITGA4 CAGATGCAGGATCGGAAAGAAT ACTAACTCCTGGTCCCATACCCA LEF1 AGGGAAGGATCAGGCAACTTG AAGCGTGCCTTTCGGTGG TGFA CTCCGGGTAGATCCACTTTCCT AGAGCCTGAGCCTCCCCA WNT5B GGTGGGAAGATGAGCAGTGG GATGCTTACGTGCTGCGTGTT

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Table S2: Genes whose expression changed by more than twofold after knock down of Ezh2

Accession # Gene ID Sequence Description Fold change NM_004114 FGF13 Homo sapiens fibroblast growth factor 13 (FGF13), transcript variant 1, mRNA [NM_004114] 15.6 L33930 CD24 Homo sapiens CD24 signal transducer mRNA, complete cds and 3' region. [L33930] 8.4 NM_003108 SOX11 Homo sapiens SRY (sex determining region Y)-box 11 (SOX11), mRNA [NM_003108] 7.9 NM_002825 PTN Homo sapiens pleiotrophin (PTN), mRNA [NM_002825] 7.5 NM_018644 B3GAT1 Homo sapiens beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 2, mRNA [NM_054025] 6.8 NM_000237 LPL Homo sapiens lipoprotein lipase (LPL), mRNA [NM_000237] 6.8 Downloaded from NM_000599 IGFBP5 Homo sapiens insulin-like growth factor binding protein 5 (IGFBP5), mRNA [NM_000599] 6.6 AK126620 FAT3 Homo sapiens FAT tumor suppressor homolog 3 (Drosophila) (FAT3), mRNA [NM_001008781] 6.4 NM_152728 ENST00000323355 Uncharacterized protein C18orf20 Precursor [Source:UniProtKB/Swiss-Prot;Acc:Q7Z4B0] [ENST00000323355] 6.0 NM_000885 ITGA4 Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4), mRNA [NM_000885] 5.8 AK021467 AK021467 Homo sapiens cDNA FLJ11405 fis, clone HEMBA1000769. [AK021467] 5.7

http://hyper.ahajournals.org/ NM_000800 FGF1 Homo sapiens fibroblast growth factor 1 (acidic) (FGF1), transcript variant 1, mRNA [NM_000800] 5.6 NM_002345 LUM Homo sapiens lumican (LUM), mRNA [NM_002345] 5.4 NM_001040429 PCDH17 Homo sapiens protocadherin 17 (PCDH17), mRNA [NM_001040429] 5.1 NM_018936 PCDHB2 Homo sapiens protocadherin beta 2 (PCDHB2), mRNA [NM_018936] 5.1 AK096051 LOC348174 Homo sapiens cDNA FLJ38732 fis, clone KIDNE2010750. [AK096051] 4.7 NM_022893 BCL11A Homo sapiens B-cell CLL/lymphoma 11A ( finger protein) (BCL11A), transcript variant 1, mRNA [NM_022893] 4.7 BM680083 BM680083 BM680083 UI-E-EO1-aix-j-14-0-UI.s1 UI-E-EO1 Homo sapiens cDNA clone UI-E-EO1-aix-j-14-0-UI 3', mRNA sequence [BM680083] 4.5 NM_005382 NEFM Homo sapiens neurofilament, medium polypeptide (NEFM), transcript variant 1, mRNA [NM_005382] 4.5 BC008690 JPH3 Homo sapiens junctophilin 3, mRNA (cDNA clone IMAGE:3867947), complete cds. [BC008690] 4.5 byguest onSeptember11, 2012 T66870 THC2680667 Q9BVX4_HUMAN (Q9BVX4) MGC5566 protein, partial (23%) [THC2680667] 4.4 AK123878 MEG3 Homo sapiens cDNA FLJ41884 fis, clone OCBBF2023162. [AK123878] 4.4 AK055647 AK055647 Homo sapiens cDNA FLJ31085 fis, clone IMR321000037. [AK055647] 4.4 NM_178012 TUBB2B Homo sapiens tubulin, beta 2B (TUBB2B), mRNA [NM_178012] 4.4 NM_012464 TLL1 Homo sapiens tolloid-like 1 (TLL1), mRNA [NM_012464] 4.4 NM_004385 VCAN Homo sapiens versican (VCAN), transcript variant 1, mRNA [NM_004385] 4.3 NM_025212 CXXC4 Homo sapiens CXXC finger 4 (CXXC4), mRNA [NM_025212] 4.3 BC035246 RIMBP3 Homo sapiens RIMS binding protein 3 (RIMBP3), mRNA [NM_015672] 4.3 NM_030926 ITM2C Homo sapiens integral membrane protein 2C (ITM2C), transcript variant 1, mRNA [NM_030926] 4.2 NM_004086 COCH Homo sapiens coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2, mRNA [NM_004086] 4.2 NM_015689 DENND2A Homo sapiens DENN/MADD domain containing 2A (DENND2A), mRNA [NM_015689] 4.2 NM_030955 ADAMTS12 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif, 12 (ADAMTS12), mRNA [NM_030955] 4.2 NM_018930 PCDHB10 Homo sapiens protocadherin beta 10 (PCDHB10), mRNA [NM_018930] 4.2 NM_015669 PCDHB5 Homo sapiens protocadherin beta 5 (PCDHB5), mRNA [NM_015669] 4.1 NM_152679 SLC10A4 Homo sapiens solute carrier family 10 (sodium/bile acid cotransporter family), member 4 (SLC10A4), mRNA [NM_152679] 4.1 NM_053036 NPFFR2 Homo sapiens neuropeptide FF receptor 2 (NPFFR2), transcript variant 2, mRNA [NM_053036] 4.1 AB011103 KIF5C Homo sapiens kinesin family member 5C (KIF5C), mRNA [NM_004522] 4.1 NM_012445 SPON2 Homo sapiens spondin 2, extracellular matrix protein (SPON2), transcript variant 1, mRNA [NM_012445] 4.0 NM_198521 C12orf42 Homo sapiens chromosome 12 open reading frame 42 (C12orf42), transcript variant 1, mRNA [NM_198521] 4.0 NM_012259 HEY2 Homo sapiens hairy/enhancer-of-split related with YRPW motif 2 (HEY2), mRNA [NM_012259] 4.0 NM_021021 SNTB1 Homo sapiens syntrophin, beta 1 (dystrophin-associated protein A1, 59kDa, basic component 1) (SNTB1), mRNA [NM_021021] 4.0 AK056142 BEAN Homo sapiens brain expressed, associated with Nedd4 (BEAN), mRNA [NM_001136106] 4.0 NM_005715 UST Homo sapiens uronyl-2-sulfotransferase (UST), mRNA [NM_005715] 3.9 NM_052913 TMEM200A Homo sapiens transmembrane protein 200A (TMEM200A), mRNA [NM_052913] 3.9 AI042308 oy13h03.x1 Soares_senescent_fibroblasts_NbHSF Homo sapiens cDNA clone IMAGE:1665749 3' similar to gb:M95787 SMOOTH MUSCLE PROTEIN 22-AL 3.9 NM_000625 NOS2 Homo sapiens nitric oxide synthase 2, inducible (NOS2), mRNA [NM_000625] 3.9 NM_002521 NPPB Homo sapiens natriuretic peptide precursor B (NPPB), mRNA [NM_002521] 3.8 AI207522 AI207522 AI207522 HA2878 Human fetal liver cDNA library Homo sapiens cDNA, mRNA sequence [AI207522] 3.8

Downloaded from BC036599 BC036599 Homo sapiens cDNA clone IMAGE:5273964. [BC036599] 3.8 NM_000089 COL1A2 Homo sapiens collagen, type I, alpha 2 (COL1A2), mRNA [NM_000089] 3.7 BQ638334 hd21c06.y1 Human Retina cDNA (Un-normalized, unamplified): hd [BQ638334] 3.7 NM_013989 DIO2 Homo sapiens deiodinase, iodothyronine, type II (DIO2), transcript variant 1, mRNA [NM_013989] 3.7 NM_000623 BDKRB2 Homo sapiens B2 (BDKRB2), mRNA [NM_000623] 3.7 NM_022137 SMOC1 Homo sapiens SPARC related modular calcium binding 1 (SMOC1), transcript variant 1, mRNA [NM_001034852] 3.7 http://hyper.ahajournals.org/ NM_019119 PCDHB9 Homo sapiens protocadherin beta 9 (PCDHB9), mRNA [NM_019119] 3.7 CD237413 BG105662 602312243F1 NIH_MGC_84 Homo sapiens cDNA clone IMAGE:4421770 5', mRNA sequence [BG105662] 3.7 NM_001299 CNN1 Homo sapiens calponin 1, basic, smooth muscle (CNN1), mRNA [NM_001299] 3.7 NM_002523 NPTX2 Homo sapiens neuronal pentraxin II (NPTX2), mRNA [NM_002523] 3.6 NM_020358 TRIM49 Homo sapiens tripartite motif-containing 49 (TRIM49), mRNA [NM_020358] 3.6 AF334588 AF334588 Homo sapiens P25 mRNA, complete cds. [AF334588] 3.6 NM_014702 KIAA0408 Homo sapiens KIAA0408 (KIAA0408), mRNA [NM_014702] 3.5 NM_000090 COL3A1 Homo sapiens collagen, type III, alpha 1 (COL3A1), mRNA [NM_000090] 3.5 byguest onSeptember11, 2012 NM_018476 BEX1 Homo sapiens brain expressed, X-linked 1 (BEX1), mRNA [NM_018476] 3.5 AI828007 AI828007 AI828007 wf12e06.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:2350402 3' similar to TR:Q92743 Q92743 NOVEL SERINE PROTEASE. ; 3.5 NM_003068 SNAI2 Homo sapiens snail homolog 2 (Drosophila) (SNAI2), mRNA [NM_003068] 3.5 AB188494 ENST00000399816 Probable ubiquitin-conjugating E2 FLJ25076 (EC 6.3.2.19) [Source:UniProtKB/Swiss-Prot;Acc:A1L167] [ENST00000399816] 3.5 NM_153350 FBXL16 Homo sapiens F-box and leucine-rich repeat protein 16 (FBXL16), mRNA [NM_153350] 3.4 BC033223 SLC45A4 Homo sapiens solute carrier family 45, member 4, mRNA (cDNA clone IMAGE:5019517), complete cds. [BC033223] 3.4 NM_005544 IRS1 Homo sapiens insulin receptor substrate 1 (IRS1), mRNA [NM_005544] 3.4 NM_002025 AFF2 Homo sapiens AF4/FMR2 family, member 2 (AFF2), mRNA [NM_002025] 3.4 NCAM2 Neural cell adhesion molecule 2 Precursor (N-CAM 2) [Source:UniProtKB/Swiss-Prot;Acc:O15394] [ENST00000400546] 3.4 NM_024888 PRG2 Homo sapiens plasticity-related gene 2 (PRG2), mRNA [NM_024888] 3.4 NM_138711 PPARG Homo sapiens peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 3, mRNA [NM_138711] 3.4 BM998048 BM998048 UI-H-DT1-avx-f-03-0-UI.s1 NCI_CGAP_DT1 Homo sapiens cDNA clone IMAGE:5885570 3', mRNA sequence [BM998048] 3.4 NM_003236 TGFA Homo sapiens transforming growth factor, alpha (TGFA), transcript variant 1, mRNA [NM_003236] 3.4 NM_181538 GJC3 Homo sapiens gap junction protein, gamma 3, 30.2kDa (GJC3), mRNA [NM_181538] 3.3 NM_001874 CPM Homo sapiens carboxypeptidase M (CPM), transcript variant 1, mRNA [NM_001874] 3.3 NM_031308 EPPK1 Homo sapiens epiplakin 1 (EPPK1), mRNA [NM_031308] 3.3 NM_013305 ST8SIA5 Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (ST8SIA5), mRNA [NM_013305] 3.3 NM_003956 CH25H Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA [NM_003956] 3.3 NM_001017395 TMCC1 Homo sapiens transmembrane and coiled-coil domain family 1 (TMCC1), transcript variant 1, mRNA [NM_001017395] 3.3 NM_018406 MUC4 Homo sapiens mucin 4, cell surface associated (MUC4), transcript variant 1, mRNA [NM_018406] 3.3 BC031314 BC031314 Homo sapiens cDNA clone IMAGE:5276765. [BC031314] 3.3 NM_198545 C1orf187 Homo sapiens chromosome 1 open reading frame 187 (C1orf187), mRNA [NM_198545] 3.3 NM_000488 SERPINC1 Homo sapiens serpin peptidase inhibitor, clade C (antithrombin), member 1 (SERPINC1), mRNA [NM_000488] 3.2 NM_022123 NPAS3 Homo sapiens neuronal PAS domain protein 3 (NPAS3), transcript variant 1, mRNA [NM_022123] 3.2 NM_003507 FZD7 Homo sapiens frizzled homolog 7 (Drosophila) (FZD7), mRNA [NM_003507] 3.2 NM_014867 KBTBD11 Homo sapiens kelch repeat and BTB (POZ) domain containing 11 (KBTBD11), mRNA [NM_014867] 3.2 CN479762 CN479762 CN479762 UI-H-EU0-azu-l-04-0-UI.s1 NCI_CGAP_Car1 Homo sapiens cDNA clone UI-H-EU0-azu-l-04-0-UI 3', mRNA sequence [CN479762] 3.2 BX647987 BX647987 Homo sapiens mRNA; cDNA DKFZp686N1452 (from clone DKFZp686N1452). [BX647987] 3.2 NM_145306 C10orf35 Homo sapiens chromosome 10 open reading frame 35 (C10orf35), mRNA [NM_145306] 3.2

Downloaded from NM_020634 GDF3 Homo sapiens growth differentiation factor 3 (GDF3), mRNA [NM_020634] 3.2 CR936791 FAM169A Homo sapiens family with sequence similarity 169, member A (FAM169A), mRNA [NM_015566] 3.1 BC002889 ZNF224 Homo sapiens protein 224, mRNA (cDNA clone IMAGE:3941350), partial cds. [BC002889] 3.1 NM_015381 FAM19A5 Homo sapiens family with sequence similarity 19 (chemokine (C-C motif)-like), member A5 (FAM19A5), transcript variant 2, mRNA [NM_015381] 3.1 CR593560 LOC440934 full-length cDNA clone CS0DC029YA18 of Neuroblastoma Cot 25-normalized of Homo sapiens (human). [CR593560] 3.1 NM_152677 ZSCAN4 Homo sapiens zinc finger and SCAN domain containing 4 (ZSCAN4), mRNA [NM_152677] 3.1 http://hyper.ahajournals.org/ NM_000641 IL11 Homo sapiens interleukin 11 (IL11), mRNA [NM_000641] 3.1 NM_020957 PCDHB16 Homo sapiens protocadherin beta 16 (PCDHB16), mRNA [NM_020957] 3.1 NM_017549 EPDR1 Homo sapiens ependymin related protein 1 () (EPDR1), mRNA [NM_017549] 3.1 AK021874 TGFB2 Homo sapiens transforming growth factor, beta 2 (TGFB2), transcript variant 1, mRNA [NM_001135599] 3.1 CA432213 CA432213 CA432213 UI-H-FG1-bgr-l-15-0-UI.s1 NCI_CGAP_FG1 Homo sapiens cDNA clone UI-H-FG1-bgr-l-15-0-UI 3', mRNA sequence [CA432213] 3.1 AK023723 HS3ST3B1 Heparan sulfate glucosamine 3-O-sulfotransferase 3B1 (EC 2.8.2.30)(Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1)(Heparan sulfate 3-O-sulfotra 3.1 NM_031310 PLVAP Homo sapiens plasmalemma vesicle associated protein (PLVAP), mRNA [NM_031310] 3.1 AF199613 FGF13 Homo sapiens fibroblast growth factor 13 (FGF13), transcript variant 4, mRNA [NM_001139498] 3.1 byguest onSeptember11, 2012 NM_016651 DACT1 Homo sapiens dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1, mRNA [NM_016651] 3.1 NM_030820 COL21A1 Homo sapiens collagen, type XXI, alpha 1 (COL21A1), mRNA [NM_030820] 3.0 AK096495 RNF152 Homo sapiens cDNA FLJ39176 fis, clone OCBBF2003744, weakly similar to ZINC-FINGER PROTEIN HT2A. [AK096495] 3.0 THC2664860 Q9SI74_ARATH (Q9SI74) F23N19.12, partial (5%) [THC2664860] 3.0 NM_006909 RASGRF2 Homo sapiens Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mRNA [NM_006909] 3.0 NM_015686 FAM155B Homo sapiens family with sequence similarity 155, member B (FAM155B), mRNA [NM_015686] 3.0 NM_015236 LPHN3 Homo sapiens 3 (LPHN3), mRNA [NM_015236] 3.0 NM_138415 PHF21B Homo sapiens PHD finger protein 21B (PHF21B), transcript variant 1, mRNA [NM_138415] 3.0 NM_001018084 SLC26A10 Homo sapiens cDNA FLJ16735 fis, clone BRACE2005457, weakly similar to PENDRIN. [AK122981] 3.0 AK055101 C6orf168 Homo sapiens open reading frame 168 (C6orf168), mRNA [NM_032511] 3.0 NM_181482 C18orf1 Homo sapiens chromosome 18 open reading frame 1 (C18orf1), transcript variant a2, mRNA [NM_181482] 3.0 NM_004538 NAP1L3 Homo sapiens nucleosome assembly protein 1-like 3 (NAP1L3), mRNA [NM_004538] 3.0 NM_022166 XYLT1 Homo sapiens xylosyltransferase I (XYLT1), mRNA [NM_022166] 2.9 NM_033056 PCDH15 Homo sapiens protocadherin 15 (PCDH15), transcript variant C, mRNA [NM_033056] 2.9 NM_024007 EBF1 Homo sapiens early B-cell factor 1 (EBF1), mRNA [NM_024007] 2.9 NM_207334 FAM43B Homo sapiens family with sequence similarity 43, member B (FAM43B), mRNA [NM_207334] 2.9 NM_144668 WDR66 Homo sapiens WD repeat domain 66 (WDR66), mRNA [NM_144668] 2.9 NM_153244 C10orf111 Homo sapiens chromosome 10 open reading frame 111 (C10orf111), mRNA [NM_153244] 2.9 NM_018934 PCDHB14 Homo sapiens protocadherin beta 14 (PCDHB14), mRNA [NM_018934] 2.9 NM_019849 SLC7A10 Homo sapiens solute carrier family 7, (neutral transporter, y+ system) member 10 (SLC7A10), mRNA [NM_019849] 2.9 NM_024522 NKAIN1 Homo sapiens Na+/K+ transporting ATPase interacting 1 (NKAIN1), mRNA [NM_024522] 2.9 NM_198391 FLRT3 Homo sapiens fibronectin leucine rich transmembrane protein 3 (FLRT3), transcript variant 2, mRNA [NM_198391] 2.9 NM_001025266 C3orf70 Homo sapiens chromosome 3 open reading frame 70 (C3orf70), mRNA [NM_001025266] 2.9 NM_002425 MMP10 Homo sapiens matrix metallopeptidase 10 (stromelysin 2) (MMP10), mRNA [NM_002425] 2.9 AK124281 AK124281 Homo sapiens cDNA FLJ42287 fis, clone TLIVE2005866. [AK124281] 2.9 NM_012190 ALDH1L1 Homo sapiens aldehyde dehydrogenase 1 family, member L1 (ALDH1L1), mRNA [NM_012190] 2.9 NM_003734 AOC3 Homo sapiens amine oxidase, copper containing 3 (vascular adhesion protein 1) (AOC3), mRNA [NM_003734] 2.9 FREM3 FRAS1-related extracellular matrix protein 3 Precursor [Source:UniProtKB/Swiss-Prot;Acc:P0C091] [ENST00000329798] 2.9 NM_052813 CARD9 Homo sapiens caspase recruitment domain family, member 9 (CARD9), transcript variant 1, mRNA [NM_052813] 2.9

Downloaded from NR_002307 MSX2P1 Homo sapiens msh 2 pseudogene 1 (MSX2P1), non-coding RNA [NR_002307] 2.9 NM_032446 MEGF10 Homo sapiens multiple EGF-like-domains 10 (MEGF10), mRNA [NM_032446] 2.9 NM_001013398 IGFBP3 Homo sapiens insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 1, mRNA [NM_001013398] 2.9 AK122886 ENST00000372142 Pro-neuregulin-3, membrane-bound isoform Precursor (Pro-NRG3) [Contains Neuregulin-3(NRG-3)] [Source:UniProtKB/Swiss-Prot;Acc:P56975] [ENST00000 2.9 NM_018440 PAG1 Homo sapiens phosphoprotein associated with glycosphingolipid microdomains 1 (PAG1), mRNA [NM_018440] 2.9 NM_003238 TGFB2 Homo sapiens transforming growth factor, beta 2 (TGFB2), transcript variant 2, mRNA [NM_003238] 2.9 http://hyper.ahajournals.org/ NM_031303 KATNAL2 Homo sapiens katanin p60 subunit A-like 2 (KATNAL2), mRNA [NM_031303] 2.8 NM_198270 NHS Homo sapiens Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant 1, mRNA [NM_198270] 2.8 AK098753 MIAT Homo sapiens myocardial infarction associated transcript (non-protein coding) (MIAT), non-coding RNA [NR_003491] 2.8 BC038245 BC038245 Homo sapiens, clone IMAGE:5241654, mRNA. [BC038245] 2.8 CR740121 CR740121 CR740121 Homo sapiens library (Ebert L) Homo sapiens cDNA clone IMAGp971G1750 ; IMAGE:767753 5', mRNA sequence [CR740121] 2.8 NM_052862 RCSD1 Homo sapiens RCSD domain containing 1 (RCSD1), mRNA [NM_052862] 2.8 THC2647739 HUMITGA4A integrin alpha 4 subunit {Homo sapiens} (exp=-1; wgp=0; cg=0), partial (90%) [THC2647739] 2.8 NM_053064 GNG2 Homo sapiens guanine nucleotide binding protein (G protein), gamma 2 (GNG2), mRNA [NM_053064] 2.8 byguest onSeptember11, 2012 NM_006475 POSTN Homo sapiens periostin, osteoblast specific factor (POSTN), transcript variant 1, mRNA [NM_006475] 2.8 NM_018935 PCDHB15 Homo sapiens protocadherin beta 15 (PCDHB15), mRNA [NM_018935] 2.8 AK126405 CMKLR1 Homo sapiens chemokine-like receptor 1 (CMKLR1), transcript variant 1, mRNA [NM_001142343] 2.8 NM_006528 TFPI2 Homo sapiens tissue factor pathway inhibitor 2 (TFPI2), mRNA [NM_006528] 2.8 NM_030775 WNT5B Homo sapiens wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2, mRNA [NM_030775] 2.8 NM_005025 SERPINI1 Homo sapiens serpin peptidase inhibitor, clade I (neuroserpin), member 1 (SERPINI1), transcript variant 1, mRNA [NM_005025] 2.8 NM_003436 ZNF135 Homo sapiens zinc finger protein 135 (ZNF135), mRNA [NM_003436] 2.8 NM_004750 CRLF1 Homo sapiens cytokine receptor-like factor 1 (CRLF1), mRNA [NM_004750] 2.8 NM_018271 THNSL2 Homo sapiens threonine synthase-like 2 (S. cerevisiae) (THNSL2), mRNA [NM_018271] 2.8 AI652920 BE669520 7e14b03.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE:3282413 3' similar to SW:NMA_HUMAN Q13145 PUTATIVE TRANSMEMBRANE PROTEIN 2.8 BX648591 BX648591 Homo sapiens mRNA; cDNA DKFZp686G14198 (from clone DKFZp686G14198). [BX648591] 2.8 NM_016352 CPA4 Homo sapiens carboxypeptidase A4 (CPA4), mRNA [NM_016352] 2.8 NM_004867 ITM2A Homo sapiens integral membrane protein 2A (ITM2A), mRNA [NM_004867] 2.7 AW138903 AW138903 AW138903 UI-H-BI1-aeq-e-09-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone IMAGE:2720344 3', mRNA sequence [AW138903] 2.7 BX095557 BX095557 BX095557 Soares_testis_NHT Homo sapiens cDNA clone IMAGp998I142576, mRNA sequence [BX095557] 2.7 BC014604 LOC728613 Homo sapiens programmed cell death 6 pseudogene (LOC728613), non-coding RNA [NR_003713] 2.7 NM_004770 KCNB2 Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 2 (KCNB2), mRNA [NM_004770] 2.7 AK094799 AK094799 Homo sapiens cDNA FLJ37480 fis, clone BRAWH2013866, highly similar to Homo sapiens stonin 2 mRNA. [AK094799] 2.7 NM_018689 KIAA1199 Homo sapiens KIAA1199 (KIAA1199), mRNA [NM_018689] 2.7 NM_006727 CDH10 Homo sapiens cadherin 10, type 2 (T2-cadherin) (CDH10), mRNA [NM_006727] 2.7 NM_003933 BAIAP3 Homo sapiens BAI1-associated protein 3 (BAIAP3), mRNA [NM_003933] 2.7 NM_005070 SLC4A3 Homo sapiens solute carrier family 4, anion exchanger, member 3 (SLC4A3), transcript variant 1, mRNA [NM_005070] 2.7 NM_182595 DKFZp564N2472 Homo sapiens hypothetical protein DKFZp564N2472 (DKFZp564N2472), mRNA [NM_182595] 2.7 NM_024572 GALNT14 Homo sapiens UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14) (GALNT14), mRNA [NM_024572] 2.7 NM_138715 MSR1 Homo sapiens macrophage scavenger receptor 1 (MSR1), transcript variant SR-AI, mRNA [NM_138715] 2.7 NM_004476 FOLH1 Homo sapiens folate hydrolase (prostate-specific membrane antigen) 1 (FOLH1), transcript variant 1, mRNA [NM_004476] 2.7 AK001019 KIF26B Homo sapiens kinesin family member 26B (KIF26B), mRNA [NM_018012] 2.7 LOC100130703 PREDICTED: Homo sapiens similar to hCG2042168 (LOC100130703), mRNA [XM_001714375] 2.7 AK090431 NLRC3 Homo sapiens NLR family, CARD domain containing 3 (NLRC3), mRNA [NM_178844] 2.7

Downloaded from NM_004861 GAL3ST1 Homo sapiens galactose-3-O-sulfotransferase 1 (GAL3ST1), mRNA [NM_004861] 2.7 NM_032932 RAB11FIP4 Homo sapiens RAB11 family interacting protein 4 (class II) (RAB11FIP4), mRNA [NM_032932] 2.7 AI754733 TGFBI AL573456 Homo sapiens PLACENTA COT 25-NORMALIZED Homo sapiens cDNA clone CS0DI051YC22 3-PRIME, mRNA sequence [AL573456] 2.7 AK127347 LOC100130729 Homo sapiens cDNA FLJ45419 fis, clone BRHIP3035222. [AK127347] 2.7 NM_000963 PTGS2 Homo sapiens prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mRNA [NM_000963] 2.7 AK055463 FLJ30901 Homo sapiens cDNA FLJ30901 fis, clone FEBRA2005778. [AK055463] 2.7 http://hyper.ahajournals.org/ THC2603239 Q9NJB6_TRYBR (Q9NJB6) Fibrillarin, partial (10%) [THC2603239] 2.7 THC2522889 GPR27_HUMAN (Q9NS67) Probable G-protein coupled receptor 27 (Super conserved receptor expressed in brain 1), complete [THC2522889] 2.7 NM_016269 LEF1 Homo sapiens lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mRNA [NM_016269] 2.7 NM_014862 ARNT2 Homo sapiens aryl-hydrocarbon receptor nuclear translocator 2 (ARNT2), mRNA [NM_014862] 2.7 BC104421 BC104421 Homo sapiens cDNA clone IMAGE:40004940. [BC104421] 2.7 NM_019066 MAGEL2 Homo sapiens MAGE-like 2 (MAGEL2), mRNA [NM_019066] 2.7 NM_004329 BMPR1A Homo sapiens bone morphogenetic protein receptor, type IA (BMPR1A), mRNA [NM_004329] 2.6 NM_032621 BEX2 Homo sapiens brain expressed X-linked 2 (BEX2), mRNA [NM_032621] 2.6 byguest onSeptember11, 2012 AK131454 ASXL3 Homo sapiens additional sex combs like 3 (Drosophila) (ASXL3), mRNA [NM_030632] 2.6 NM_173484 KLF17 Homo sapiens Kruppel-like factor 17 (KLF17), mRNA [NM_173484] 2.6 AL137382 LOC146429 Homo sapiens mRNA; cDNA DKFZp434L1226 (from clone DKFZp434L1226). [AL137382] 2.6 BC030100 BC030100 Homo sapiens cDNA clone IMAGE:4796595. [BC030100] 2.6 NM_138325 PCSK6 Homo sapiens proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 6, mRNA [NM_138325] 2.6 CR606754 IGSF5 Homo sapiens immunoglobulin superfamily, member 5 (IGSF5), mRNA [NM_001080444] 2.6 AB028971 ENST00000406297 AP2-associated protein kinase 1 (EC 2.7.11.1)(Adaptor-associated kinase 1) [Source:UniProtKB/Swiss-Prot;Acc:Q2M2I8] [ENST00000406297] 2.6 BC020867 SLC6A13 Homo sapiens solute carrier family 6 (neurotransmitter transporter, GABA), member 13, mRNA (cDNA clone IMAGE:4594185), complete cds. [BC020867] 2.6 NM_024645 ZMAT4 Homo sapiens zinc finger, matrin type 4 (ZMAT4), transcript variant 1, mRNA [NM_024645] 2.6 NM_024025 DUSP26 Homo sapiens dual specificity phosphatase 26 (putative) (DUSP26), mRNA [NM_024025] 2.6 NM_004443 EPHB3 Homo sapiens EPH receptor B3 (EPHB3), mRNA [NM_004443] 2.6 NM_001001522 TAGLN Homo sapiens transgelin (TAGLN), transcript variant 1, mRNA [NM_001001522] 2.6 NM_002606 PDE9A Homo sapiens phosphodiesterase 9A (PDE9A), transcript variant 1, mRNA [NM_002606] 2.6 BX115087 BX115087 NCI_CGAP_Lu5 Homo sapiens cDNA clone IMAGp998G214685, mRNA sequence [BX115087] 2.6 NM_032208 ANTXR1 Homo sapiens anthrax toxin receptor 1 (ANTXR1), transcript variant 1, mRNA [NM_032208] 2.6 NM_006686 ACTL7B Homo sapiens actin-like 7B (ACTL7B), mRNA [NM_006686] 2.6 NM_153020 RBM24 Homo sapiens RNA binding motif protein 24 (RBM24), mRNA [NM_153020] 2.6 BX113452 BX113452 BX113452 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGp998L21165, mRNA sequence [BX113452] 2.6 NM_000222 KIT Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 1, mRNA [NM_000222] 2.6 NM_032510 PARD6G Homo sapiens par-6 partitioning defective 6 homolog gamma (C. elegans) (PARD6G), mRNA [NM_032510] 2.6 NM_153696 PSMAL Homo sapiens growth-inhibiting protein 26 (PSMAL), mRNA [NM_153696] 2.6 NM_152495 CNIH3 Homo sapiens cornichon homolog 3 (Drosophila) (CNIH3), mRNA [NM_152495] 2.6 NM_194298 SLC16A9 Homo sapiens solute carrier family 16, member 9 (monocarboxylic acid transporter 9) (SLC16A9), mRNA [NM_194298] 2.6 NM_033543 CEACAM21 Homo sapiens carcinoembryonic antigen-related cell adhesion molecule 21 (CEACAM21), transcript variant 2, mRNA [NM_033543] 2.6 NM_021706 LAIR1 Homo sapiens leukocyte-associated immunoglobulin-like receptor 1 (LAIR1), transcript variant a, mRNA [NM_002287] 2.6 NM_001024660 KALRN Homo sapiens kalirin, RhoGEF kinase (KALRN), transcript variant 1, mRNA [NM_001024660] 2.6 NM_006486 FBLN1 Homo sapiens fibulin 1 (FBLN1), transcript variant D, mRNA [NM_006486] 2.6 NM_178456 C20orf85 Homo sapiens chromosome 20 open reading frame 85 (C20orf85), mRNA [NM_178456] 2.6 NM_001615 ACTG2 Homo sapiens actin, gamma 2, smooth muscle, enteric (ACTG2), mRNA [NM_001615] 2.6

Downloaded from NM_153027 C4orf39 Homo sapiens chromosome 4 open reading frame 39 (C4orf39), mRNA [NM_153027] 2.6 NM_001007139 IGF2 Homo sapiens insulin-like growth factor 2 (somatomedin A) (IGF2), transcript variant 1, mRNA [NM_000612] 2.6 NM_000427 LOR Homo sapiens loricrin (LOR), mRNA [NM_000427] 2.6 BC078172 BC078172 Homo sapiens cDNA clone IMAGE:5760022, partial cds. [BC078172] 2.6 NM_145038 C2orf39 Homo sapiens open reading frame 39 (C2orf39), mRNA [NM_145038] 2.6 BC042853 ABCA17P Homo sapiens cDNA clone IMAGE:5271371. [BC042853] 2.6 http://hyper.ahajournals.org/ NM_031890 CECR6 Homo sapiens cat eye syndrome chromosome region, candidate 6 (CECR6), mRNA [NM_031890] 2.5 XM_933439 PPP1R1C Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1C (PPP1R1C), mRNA [NM_001080545] 2.5 AB073660 C8orf5 Homo sapiens primary neuroblastoma cDNA, clone:Nbla00437, full insert sequence. [AB073660] 2.5 NM_001257 CDH13 Homo sapiens cadherin 13, H-cadherin (heart) (CDH13), mRNA [NM_001257] 2.5 CR624679 ZNF681 Homo sapiens zinc finger protein 681 (ZNF681), mRNA [NM_138286] 2.5 NM_022068 FAM38B Homo sapiens family with sequence similarity 38, member B (FAM38B), mRNA [NM_022068] 2.5 AK055279 UTP23 Homo sapiens cDNA FLJ30717 fis, clone FCBBF2001672. [AK055279] 2.5 AI085826 BX413550 BX413550 Homo sapiens FETAL BRAIN Homo sapiens cDNA clone CS0DF026YM21 3-PRIME, mRNA sequence [BX413550] 2.5 byguest onSeptember11, 2012 BX117479 BX117479 BX117479 NCI_CGAP_Lu19 Homo sapiens cDNA clone IMAGp998I075985, mRNA sequence [BX117479] 2.5 BU617889 AA883249 AA883249 aj14b01.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone IMAGE:1390249 3' similar to contains element PTR5 repetitive element 2.5 AK094659 LOC100130078 Homo sapiens cDNA FLJ37340 fis, clone BRAMY2020574. [AK094659] 2.5 NM_018004 TMEM45A Homo sapiens transmembrane protein 45A (TMEM45A), mRNA [NM_018004] 2.5 NM_000618 IGF1 Homo sapiens insulin-like growth factor 1 (somatomedin C) (IGF1), transcript variant 4, mRNA [NM_000618] 2.5 NM_000216 KAL1 Homo sapiens Kallmann syndrome 1 sequence (KAL1), mRNA [NM_000216] 2.5 NM_153360 APCDD1L Homo sapiens adenomatosis polyposis coli down-regulated 1-like (APCDD1L), mRNA [NM_153360] 2.5 NM_001010925 ANKRD19 Homo sapiens cDNA FLJ36178 fis, clone TESTI2026534. [AK093497] 2.5 NM_172364 CACNA2D4 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4), mRNA [NM_172364] 2.5 AA989132 AA989132 oq89a02.s1 NCI_CGAP_Kid6 Homo sapiens cDNA clone IMAGE:1593482 3' similar to gb:L13923 FIBRILLIN 1 PRECURSOR (HUMAN);, mRNA sequence [A 2.5 NM_024603 BEND5 Homo sapiens BEN domain containing 5 (BEND5), mRNA [NM_024603] 2.5 U39657 MAP2K6 Human MAP kinase kinase 6 (MKK6) mRNA, complete cds. [U39657] 2.5 NM_145235 FANK1 Homo sapiens fibronectin type III and ankyrin repeat domains 1 (FANK1), mRNA [NM_145235] 2.5 NM_001845 COL4A1 Homo sapiens collagen, type IV, alpha 1 (COL4A1), mRNA [NM_001845] 2.5 NM_016173 HEMK1 Homo sapiens HemK methyltransferase family member 1 (HEMK1), mRNA [NM_016173] 2.5 NM_174953 ATP2A3 Homo sapiens ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 5, mRNA [NM_174953] 2.5 NM_001442 FABP4 Homo sapiens fatty acid binding protein 4, adipocyte (FABP4), mRNA [NM_001442] 2.5 ZNF812 Homo sapiens cDNA FLJ60289 complete cds, highly similar to Zinc finger protein 561. [AK296815] 2.5 BC045573 HNRNPM Homo sapiens heterogeneous nuclear ribonucleoprotein M, mRNA (cDNA clone IMAGE:4829644). [BC045573] 2.5 NM_001003395 TPD52L1 Homo sapiens tumor protein D52-like 1 (TPD52L1), transcript variant 2, mRNA [NM_001003395] 2.5 NM_080743 SRrp35 Homo sapiens serine-arginine repressor protein (35 kDa) (SRrp35), mRNA [NM_080743] 2.5 NM_014668 GREB1 Homo sapiens GREB1 protein (GREB1), transcript variant a, mRNA [NM_014668] 2.5 BM129308 BM129308 if20d02.x1 Melton Normalized Human Islet 4 N4-HIS 1 Homo sapiens cDNA clone IMAGE:5677082 3', mRNA sequence [BM129308] 2.5 N52197 BX455216 BX455216 Homo sapiens FETAL BRAIN Homo sapiens cDNA clone CS0DF018YB15 3-PRIME, mRNA sequence [BX455216] 2.5 BF761348 BF761348 BF761348 RC2-CS0018-041000-015-g01 CS0018 Homo sapiens cDNA, mRNA sequence [BF761348] 2.5 NM_001004439 ITGA11 Homo sapiens integrin, alpha 11 (ITGA11), mRNA [NM_001004439] 2.5 NM_001039580 MAP9 Homo sapiens microtubule-associated protein 9 (MAP9), mRNA [NM_001039580] 2.5 NR_002830 LOC492303 Homo sapiens FAM51A1 pseudogene (LOC492303), non-coding RNA [NR_002830] 2.5 BU540282 BU540282 AGENCOURT_10326456 NIH_MGC_141 Homo sapiens cDNA clone IMAGE:6571686 5', mRNA sequence [BU540282] 2.5

Downloaded from NM_024812 BAALC Homo sapiens brain and acute leukemia, cytoplasmic (BAALC), transcript variant 1, mRNA [NM_024812] 2.5 NM_032812 PLXDC2 Homo sapiens plexin domain containing 2 (PLXDC2), mRNA [NM_032812] 2.5 BC006530 BC006530 Homo sapiens hypothetical LOC554175, mRNA (cDNA clone MGC:11077 IMAGE:3688915), complete cds. [BC006530] 2.5 THC2638097 Q3MHD6_HUMAN (Q3MHD6) COX17 homolog, oxidase assembly protein, complete [THC2638097] 2.5 NM_152737 RNF182 Homo sapiens ring finger protein 182 (RNF182), mRNA [NM_152737] 2.5 THC2713795 Q9F8M7_CARHY (Q9F8M7) DTDP-glucose 4,6-dehydratase (Fragment), partial (11%) [THC2713795] 2.5 http://hyper.ahajournals.org/ THC2520461 Q455W3_9BURK (Q455W3) Aldehyde dehydrogenase, partial (4%) [THC2520461] 2.5 BC011643 AUTS2 Homo sapiens autism susceptibility candidate 2 (AUTS2), transcript variant 3, mRNA [NM_001127232] 2.5 NM_014505 KCNMB4 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, beta member 4 (KCNMB4), mRNA [NM_014505] 2.5 BX375060 BX375060 BX375060 Homo sapiens NEUROBLASTOMA COT 25-NORMALIZED Homo sapiens cDNA clone CS0DC007YF13 5-PRIME, mRNA sequence [BX375060] 2.5 NM_000814 GABRB3 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 1, mRNA [NM_000814] 2.4 NM_005378 MYCN Homo sapiens v- myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN), mRNA [NM_005378] 2.4 NM_153235 TXLNB Homo sapiens taxilin beta (TXLNB), mRNA [NM_153235] 2.4 NM_014419 DKKL1 Homo sapiens dickkopf-like 1 (soggy) (DKKL1), mRNA [NM_014419] 2.4 byguest onSeptember11, 2012 THC2702298 ALU1_HUMAN (P39188) Alu subfamily J sequence contamination warning entry, partial (6%) [THC2702298] 2.4 THC2746246 Q9TD22_9SMEG (Q9TD22) NADH dehydrogenase subunit II, partial (5%) [THC2746246] 2.4 NM_006399 BATF Homo sapiens basic , ATF-like (BATF), mRNA [NM_006399] 2.4 NM_005639 SYT1 Homo sapiens synaptotagmin I (SYT1), transcript variant 1, mRNA [NM_005639] 2.4 AK026826 ITGA9 Homo sapiens cDNA: FLJ23173 fis, clone LNG10019. [AK026826] 2.4 NM_024835 GGNBP2 Homo sapiens gametogenetin binding protein 2 (GGNBP2), mRNA [NM_024835] 2.4 NM_015053 PPFIA4 Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (PPFIA4), mRNA [NM_015053] 2.4 NM_057157 CYP26A1 Homo sapiens cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 2, mRNA [NM_057157] 2.4 BQ441611 BQ441611 AGENCOURT_7828644 NIH_MGC_82 Homo sapiens cDNA clone IMAGE:6102021 5', mRNA sequence [BQ441611] 2.4 NM_003279 TNNC2 Homo sapiens troponin C type 2 (fast) (TNNC2), mRNA [NM_003279] 2.4 NM_001482 GATM Homo sapiens glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein, mRNA [NM_001482] 2.4 NM_032342 C9orf125 Homo sapiens open reading frame 125 (C9orf125), mRNA [NM_032342] 2.4 NM_017551 GRID1 Homo sapiens , ionotropic, delta 1 (GRID1), mRNA [NM_017551] 2.4 AL832834 LOC653284 Homo sapiens mRNA; cDNA DKFZp667O0524 (from clone DKFZp667O0524). [AL832834] 2.4 NM_000775 CYP2J2 Homo sapiens cytochrome P450, family 2, subfamily J, polypeptide 2 (CYP2J2), mRNA [NM_000775] 2.4 NM_018932 PCDHB12 Homo sapiens protocadherin beta 12 (PCDHB12), mRNA [NM_018932] 2.4 AF086511 AF086511 Homo sapiens full length insert cDNA clone ZE03A08. [AF086511] 2.4 NM_144966 FREM1 Homo sapiens FRAS1 related extracellular matrix 1 (FREM1), mRNA [NM_144966] 2.4 DB360728 DB360728 DB360728 MAMMA1 Homo sapiens cDNA clone MAMMA1000535 3', mRNA sequence [DB360728] 2.4 AB064667 OK/SW-CL.58 Homo sapiens mRNA for OK/SW-CL.58, complete cds. [AB064667] 2.4 AB051463 TET1 Homo sapiens tet oncogene 1 (TET1), mRNA [NM_030625] 2.4 XM_932357 THC2500237 CB027_HUMAN (Q580R0) Protein C2orf27, partial (79%) [THC2500237] 2.4 NM_000855 GUCY1A2 Homo sapiens guanylate cyclase 1, soluble, alpha 2 (GUCY1A2), mRNA [NM_000855] 2.4 NM_001759 CCND2 Homo sapiens cyclin D2 (CCND2), mRNA [NM_001759] 2.4 NM_021785 RAI2 Homo sapiens retinoic acid induced 2 (RAI2), mRNA [NM_021785] 2.4 NM_001406 EFNB3 Homo sapiens ephrin-B3 (EFNB3), mRNA [NM_001406] 2.4 NM_004370 COL12A1 Homo sapiens collagen, type XII, alpha 1 (COL12A1), transcript variant long, mRNA [NM_004370] 2.4 NM_018962 DSCR6 Homo sapiens Down syndrome critical region gene 6 (DSCR6), mRNA [NM_018962] 2.4 AK131325 PRAMEL Homo sapiens cDNA FLJ16327 fis, clone SYNOV1000256, weakly similar to Human preferentially expressed antigen of melanoma (PRAME) mRNA. [AK1313 2.4

Downloaded from NM_078487 CDKN2B Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 2, mRNA [NM_078487] 2.4 NM_004933 CDH15 Homo sapiens cadherin 15, type 1, M-cadherin (myotubule) (CDH15), mRNA [NM_004933] 2.4 NM_006113 VAV3 Homo sapiens vav 3 guanine nucleotide exchange factor (VAV3), transcript variant 1, mRNA [NM_006113] 2.4 AK097804 C20orf112 Homo sapiens cDNA FLJ40485 fis, clone TESTI2043857, moderately similar to Homo sapiens nolp mRNA. [AK097804] 2.4 NM_002354 EPCAM Homo sapiens epithelial cell adhesion molecule (EPCAM), mRNA [NM_002354] 2.4 THC2622086 HSA277441 xylosyltransferase I {Homo sapiens} (exp=-1; wgp=0; cg=0), partial (8%) [THC2622086] 2.4 http://hyper.ahajournals.org/ CR610949 CR610949 full-length cDNA clone CS0DD005YM02 of Neuroblastoma Cot 50-normalized of Homo sapiens (human). [CR610949] 2.4 NM_003671 CDC14B Homo sapiens CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 2, mRNA [NM_033331] 2.4 AK055439 LOC256021 Homo sapiens cDNA FLJ30877 fis, clone FEBRA2004443. [AK055439] 2.4 NM_145056 DACT3 Homo sapiens dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3), mRNA [NM_145056] 2.4 BI261159 BI261159 602968272F1 NIH_MGC_12 Homo sapiens cDNA clone IMAGE:5107840 5', mRNA sequence [BI261159] 2.4 THC2676737 Q6FYH6_BARQU (Q6FYH6) Succinate dehydrogenase cytochrome b560 subunit, partial (10%) [THC2676737] 2.4 NM_198594 C1QTNF1 Homo sapiens C1q and tumor necrosis factor related protein 1 (C1QTNF1), mRNA [NM_198594] 2.4 AK094718 FLJ22536 Homo sapiens cDNA FLJ37399 fis, clone BRAMY2027587. [AK094718] 2.4 byguest onSeptember11, 2012 NM_202000 ACSM3 Homo sapiens acyl-CoA synthetase medium-chain family member 3 (ACSM3), transcript variant 2, mRNA [NM_202000] 2.4 AA719641 AA719641 AA719641 zg52f01.s1 Soares_pineal_gland_N3HPG Homo sapiens cDNA clone IMAGE:396985 3', mRNA sequence [AA719641] 2.3 NM_004072 CMKLR1 Homo sapiens chemokine-like receptor 1 (CMKLR1), transcript variant 2, mRNA [NM_004072] 2.3 BX640888 GPC6 Homo sapiens mRNA; cDNA DKFZp686H20120 (from clone DKFZp686H20120). [BX640888] 2.3 NM_013363 PCOLCE2 Homo sapiens procollagen C-endopeptidase enhancer 2 (PCOLCE2), mRNA [NM_013363] 2.3 AB051442 KIAA1655 Homo sapiens mRNA for KIAA1655 protein, partial cds. [AB051442] 2.3 NM_015363 ZIM2 Homo sapiens zinc finger, imprinted 2 (ZIM2), mRNA [NM_015363] 2.3 NM_019854 PRMT8 Homo sapiens protein arginine methyltransferase 8 (PRMT8), mRNA [NM_019854] 2.3 NM_019072 SGTB Homo sapiens small glutamine-rich tetratricopeptide repeat (TPR)-containing, beta (SGTB), mRNA [NM_019072] 2.3 NM_003105 SORL1 Homo sapiens sortilin-related receptor, L(DLR class) A repeats-containing (SORL1), mRNA [NM_003105] 2.3 CB306644 THC2677261 ALU8_HUMAN (P39195) Alu subfamily SX sequence contamination warning entry, partial (4%) [THC2677261] 2.3 NM_001100 ACTA1 Homo sapiens actin, alpha 1, skeletal muscle (ACTA1), mRNA [NM_001100] 2.3 NM_001558 IL10RA Homo sapiens interleukin 10 receptor, alpha (IL10RA), mRNA [NM_001558] 2.3 NM_015116 LRCH1 Homo sapiens leucine-rich repeats and calponin (CH) domain containing 1 (LRCH1), mRNA [NM_015116] 2.3 CD521096 THC2739760 CD521096 AGENCOURT_14355292 NIH_MGC_191 Homo sapiens cDNA clone IMAGE:30410815 5', mRNA sequence [CD521096] 2.3 NM_004321 KIF1A Homo sapiens kinesin family member 1A (KIF1A), mRNA [NM_004321] 2.3 NM_003918 GYG2 Homo sapiens glycogenin 2 (GYG2), transcript variant 2, mRNA [NM_003918] 2.3 AA906057 AA906057 AA906057 oj90d07.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:1505581 3', mRNA sequence [AA906057] 2.3 NM_005987 SPRR1A Homo sapiens small proline-rich protein 1A (SPRR1A), mRNA [NM_005987] 2.3 NM_019590 KIAA1217 Homo sapiens KIAA1217 (KIAA1217), transcript variant 1, mRNA [NM_019590] 2.3 NM_000406 GNRHR Homo sapiens gonadotropin-releasing (GNRHR), transcript variant 1, mRNA [NM_000406] 2.3 AF070541 LOC284244 Homo sapiens clones 24714 and 24715 mRNA sequence. [AF070541] 2.3 AB011146 KIAA0574 Homo sapiens KIAA0574 protein (KIAA0574), mRNA [NM_015307] 2.3 BX281073 BX281073 BX281073 BX281073 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGp998O175628 ; IMAGE:2273128, mRNA sequence [BX281073] 2.3 AK022843 LOC100131532 Homo sapiens cDNA FLJ12781 fis, clone NT2RP2001861. [AK022843] 2.3 NM_002529 NTRK1 Homo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2, mRNA [NM_002529] 2.3 NM_003458 BSN Homo sapiens bassoon (presynaptic cytomatrix protein) (BSN), mRNA [NM_003458] 2.3 BQ050540 BQ050540 AGENCOURT_6808794 NIH_MGC_71 Homo sapiens cDNA clone IMAGE:5784515 5', mRNA sequence [BQ050540] 2.3 NM_022071 SH2D4A Homo sapiens SH2 domain containing 4A (SH2D4A), mRNA [NM_022071] 2.3

Downloaded from AL049227 AL049227 Homo sapiens mRNA; cDNA DKFZp564N1116 (from clone DKFZp564N1116). [AL049227] 2.3 NM_014548 TMOD2 Homo sapiens tropomodulin 2 (neuronal) (TMOD2), transcript variant 1, mRNA [NM_014548] 2.3 NM_021136 RTN1 Homo sapiens reticulon 1 (RTN1), transcript variant 1, mRNA [NM_021136] 2.3 NM_000399 EGR2 Homo sapiens early growth response 2 (Krox-20 homolog, Drosophila) (EGR2), transcript variant 1, mRNA [NM_000399] 2.3 NM_023943 TMEM108 Homo sapiens transmembrane protein 108 (TMEM108), transcript variant 1, mRNA [NM_023943] 2.3 NM_023002 HAPLN4 Homo sapiens hyaluronan and proteoglycan link protein 4 (HAPLN4), mRNA [NM_023002] 2.3 http://hyper.ahajournals.org/ NM_032622 LNX1 Homo sapiens ligand of numb-protein X 1 (LNX1), transcript variant 2, mRNA [NM_032622] 2.3 NM_170672 RASGRP3 Homo sapiens RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2, mRNA [NM_170672] 2.3 NM_198469 MORN5 Homo sapiens MORN repeat containing 5 (MORN5), mRNA [NM_198469] 2.3 NM_032103 PPP1R12B Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 12B (PPP1R12B), transcript variant 3, mRNA [NM_032103] 2.3 NM_001179 ART3 Homo sapiens ADP-ribosyltransferase 3 (ART3), transcript variant 2, mRNA [NM_001179] 2.3 LOC729409 PREDICTED: Homo sapiens misc_RNA (LOC729409), miscRNA [XR_015594] 2.3 BU624603 BU624603 BU624603 UI-H-FG1-bgk-p-15-0-UI.s1 NCI_CGAP_FG1 Homo sapiens cDNA clone UI-H-FG1-bgk-p-15-0-UI 3', mRNA sequence [BU624603] 2.3 THC2659561 Q5WHN7_BACSK (Q5WHN7) ATPase, AAA family, partial (7%) [THC2659561] 2.3 byguest onSeptember11, 2012 NM_000439 PCSK1 Homo sapiens proprotein convertase subtilisin/kexin type 1 (PCSK1), mRNA [NM_000439] 2.3 NM_006690 MMP24 Homo sapiens matrix metallopeptidase 24 (membrane-inserted) (MMP24), mRNA [NM_006690] 2.3 NM_018990 SASH3 Homo sapiens SAM and SH3 domain containing 3 (SASH3), mRNA [NM_018990] 2.3 THC2674391 ALU8_HUMAN (P39195) Alu subfamily SX sequence contamination warning entry, partial (8%) [THC2674391] 2.3 CA424270 CA424270 CA424270 UI-H-FG0-bcw-k-12-0-UI.s1 NCI_CGAP_EN1_2 Homo sapiens cDNA clone UI-H-FG0-bcw-k-12-0-UI 3', mRNA sequence [CA424270] 2.3 NM_032387 WNK4 Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), mRNA [NM_032387] 2.3 NM_173549 C8orf47 Homo sapiens open reading frame 47 (C8orf47), mRNA [NM_173549] 2.3 NM_004439 EPHA5 Homo sapiens EPH receptor A5 (EPHA5), transcript variant 1, mRNA [NM_004439] 2.3 NM_006685 SMR3B Homo sapiens submaxillary gland androgen regulated protein 3B (SMR3B), mRNA [NM_006685] 2.3 T70285 T70285 T70285 yd26e09.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:109384 3' similar to contains Alu repetitive element;contains MER22 r 2.3 NM_006017 PROM1 Homo sapiens prominin 1 (PROM1), mRNA [NM_006017] 2.3 U80747 ENST00000398940 Mastermind-like protein 3 (Mam-3) [Source:UniProtKB/Swiss-Prot;Acc:Q96JK9] [ENST00000398940] 2.3 AK130049 AK130049 Homo sapiens cDNA FLJ26539 fis, clone KDN09310. [AK130049] 2.3 NM_005822 RCAN2 Homo sapiens regulator of calcineurin 2 (RCAN2), mRNA [NM_005822] 2.3 NM_001843 CNTN1 Homo sapiens contactin 1 (CNTN1), transcript variant 1, mRNA [NM_001843] 2.3 NM_133625 SYN2 Homo sapiens synapsin II (SYN2), transcript variant IIa, mRNA [NM_133625] 2.2 XM_097278 LOC147710 Homo sapiens hypothetical LOC147710, mRNA (cDNA clone IMAGE:40132541), partial cds. [BC127928] 2.2 NM_030915 LBH Homo sapiens limb bud and heart development homolog (mouse) (LBH), mRNA [NM_030915] 2.2 NM_014020 TMEM176B Homo sapiens transmembrane protein 176B (TMEM176B), transcript variant 1, mRNA [NM_014020] 2.2 NM_020369 FSCN3 Homo sapiens fascin homolog 3, actin-bundling protein, testicular (Strongylocentrotus purpuratus) (FSCN3), mRNA [NM_020369] 2.2 NM_003638 ITGA8 Homo sapiens integrin, alpha 8 (ITGA8), mRNA [NM_003638] 2.2 AL117454 AL117454 Homo sapiens mRNA; cDNA DKFZp586J1717 (from clone DKFZp586J1717). [AL117454] 2.2 NM_005883 APC2 Homo sapiens adenomatosis polyposis coli 2 (APC2), mRNA [NM_005883] 2.2 NM_003474 ADAM12 Homo sapiens ADAM metallopeptidase domain 12 (ADAM12), transcript variant 1, mRNA [NM_003474] 2.2 NM_002247 KCNMA1 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 2, mRNA [NM_002247] 2.2 NM_152694 ZCCHC5 Homo sapiens zinc finger, CCHC domain containing 5 (ZCCHC5), mRNA [NM_152694] 2.2 NM_000681 ADRA2A Homo sapiens adrenergic, alpha-2A-, receptor (ADRA2A), mRNA [NM_000681] 2.2 LOC100134119 PREDICTED: Homo sapiens similar to hCG2018924 (LOC100134119), mRNA [XM_001713939] 2.2 NM_017412 FZD3 Homo sapiens frizzled homolog 3 (Drosophila) (FZD3), mRNA [NM_017412] 2.2

Downloaded from NM_001203 BMPR1B Homo sapiens bone morphogenetic protein receptor, type IB (BMPR1B), mRNA [NM_001203] 2.2 NM_004441 EPHB1 Homo sapiens EPH receptor B1 (EPHB1), mRNA [NM_004441] 2.2 NM_020768 KCTD16 Homo sapiens potassium channel tetramerisation domain containing 16 (KCTD16), mRNA [NM_020768] 2.2 NM_022055 KCNK12 Homo sapiens potassium channel, subfamily K, member 12 (KCNK12), mRNA [NM_022055] 2.2 AL080082 AL080082 Homo sapiens mRNA; cDNA DKFZp564G1162 (from clone DKFZp564G1162). [AL080082] 2.2 BC007365 MGC16142 Homo sapiens hypothetical protein MGC16142, mRNA (cDNA clone IMAGE:3630861), partial cds. [BC007365] 2.2 http://hyper.ahajournals.org/ W60781 W60781 W60781 zd26f05.r1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone IMAGE:341793 5' similar to gb:J02874 FATTY ACID-BINDING PROTEIN, ADI 2.2 NM_018651 ZNF167 Homo sapiens zinc finger protein 167 (ZNF167), transcript variant 1, mRNA [NM_018651] 2.2 NM_001819 CHGB Homo sapiens chromogranin B (secretogranin 1) (CHGB), mRNA [NM_001819] 2.2 NM_018931 PCDHB11 Homo sapiens protocadherin beta 11 (PCDHB11), mRNA [NM_018931] 2.2 NM_003282 TNNI2 Homo sapiens troponin I type 2 (skeletal, fast) (TNNI2), mRNA [NM_003282] 2.2 NM_178860 SEZ6 Homo sapiens seizure related 6 homolog (mouse) (SEZ6), transcript variant 1, mRNA [NM_178860] 2.2 NM_016148 SHANK1 Homo sapiens SH3 and multiple ankyrin repeat domains 1 (SHANK1), mRNA [NM_016148] 2.2 NM_182908 DHRS2 Homo sapiens dehydrogenase/reductase (SDR family) member 2 (DHRS2), transcript variant 1, mRNA [NM_182908] 2.2 byguest onSeptember11, 2012 AF230201 TSHZ2 Teashirt homolog 2 (Zinc finger protein 218)(Ovarian cancer-related protein 10-2)(OVC10-2) [Source:UniProtKB/Swiss-Prot;Acc:Q9NRE2] [ENST0000037149 2.2 CR627133 ZNF850P Homo sapiens mRNA; cDNA DKFZp686J154 (from clone DKFZp686J154). [CR627133] 2.2 NM_001371 DNAH8 Homo sapiens dynein, axonemal, heavy chain 8 (DNAH8), mRNA [NM_001371] 2.2 NM_138576 BCL11B Homo sapiens B-cell CLL/lymphoma 11B (zinc finger protein) (BCL11B), transcript variant 1, mRNA [NM_138576] 2.2 AK026465 AK026465 Homo sapiens cDNA: FLJ22812 fis, clone KAIA2955. [AK026465] 2.2 AK074381 GAB1 Homo sapiens cDNA FLJ23801 fis, clone HEP22544. [AK074381] 2.2 NM_033181 CNR1 Homo sapiens 1 (brain) (CNR1), transcript variant 2, mRNA [NM_033181] 2.2 NM_170735 BDNF Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 1, mRNA [NM_170735] 2.2 AB371542 AB371542 pGCAP10 [AB371542] 2.2 NM_025248 SNIP Homo sapiens SNAP25-interacting protein (SNIP), mRNA [NM_025248] 2.2 BC018675 BC018675 Homo sapiens, clone IMAGE:4332461, mRNA. [BC018675] 2.2 NM_021614 KCNN2 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2), transcript variant 1, mRNA [NM_0216 2.2 NM_199461 NANOS1 Homo sapiens nanos homolog 1 (Drosophila) (NANOS1), mRNA [NM_199461] 2.2 NM_182935 MOBP Homo sapiens myelin-associated oligodendrocyte basic protein (MOBP), mRNA [NM_182935] 2.2 AF075063 AF075063 Homo sapiens full length insert cDNA YP99D02. [AF075063] 2.2 BF735554 THC2695576 BF735554 CM1-AN0080-071100-534-d05 AN0080 Homo sapiens cDNA, mRNA sequence [BF735554] 2.2 NM_000426 LAMA2 Homo sapiens laminin, alpha 2 (LAMA2), transcript variant 1, mRNA [NM_000426] 2.2 NM_053034 ANTXR1 Homo sapiens anthrax toxin receptor 1 (ANTXR1), transcript variant 2, mRNA [NM_053034] 2.2 NM_182594 ZNF454 Homo sapiens zinc finger protein 454 (ZNF454), mRNA [NM_182594] 2.2 NM_178140 PDZD2 Homo sapiens PDZ domain containing 2 (PDZD2), mRNA [NM_178140] 2.2 NM_018420 SLC22A15 Homo sapiens solute carrier family 22, member 15 (SLC22A15), mRNA [NM_018420] 2.2 NM_021641 ADAM12 Homo sapiens ADAM metallopeptidase domain 12 (ADAM12), transcript variant 2, mRNA [NM_021641] 2.2 NM_014631 SH3PXD2A Homo sapiens SH3 and PX domains 2A (SH3PXD2A), mRNA [NM_014631] 2.2 BC009635 C21orf135 Homo sapiens chromosome 21 open reading frame 135, mRNA (cDNA clone IMAGE:3891427), with apparent retained . [BC009635] 2.2 XM_929566 AW365443 AW365443 MR0-HT0072-051099-002-f02 HT0072 Homo sapiens cDNA, mRNA sequence [AW365443] 2.2 AA708933 THC2520017 AA708933 zl64d02.s1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone IMAGE:506691 3', mRNA sequence [AA708933] 2.2 NM_001039570 KREMEN1 Homo sapiens kringle containing transmembrane protein 1 (KREMEN1), transcript variant 3, mRNA [NM_001039570] 2.2 AL390175 DKFZp547K054 Homo sapiens mRNA; cDNA DKFZp547K054 (from clone DKFZp547K054). [AL390175] 2.2 NM_003692 TMEFF1 Homo sapiens transmembrane protein with EGF-like and two follistatin-like domains 1 (TMEFF1), mRNA [NM_003692] 2.2

Downloaded from NM_031491 RBP5 Homo sapiens retinol binding protein 5, cellular (RBP5), mRNA [NM_031491] 2.2 BC042026 BC042026 Homo sapiens cDNA clone IMAGE:5312689. [BC042026] 2.2 THC2643739 Q9LN52_ARATH (Q9LN52) F18O14.18, partial (6%) [THC2643739] 2.2 NM_153216 POU5F2 Homo sapiens POU domain class 5, transcription factor 2 (POU5F2), mRNA [NM_153216] 2.2 AX721195 LOC345630 Homo sapiens similar to hCG1641252 (LOC345630), non-coding RNA [NR_024356] 2.2 AK124788 GGT7 Homo sapiens cDNA FLJ42798 fis, clone BRAWH3008931. [AK124788] 2.2 http://hyper.ahajournals.org/ AK026659 AK026659 Homo sapiens cDNA: FLJ23006 fis, clone LNG00414. [AK026659] 2.2 NM_005739 RASGRP1 Homo sapiens RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1, mRNA [NM_005739] 2.2 NM_020796 SEMA6A Homo sapiens sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), mRNA [NM_020796] 2.2 AB023211 PADI2 Homo sapiens peptidyl arginine deiminase, type II (PADI2), mRNA [NM_007365] 2.2 NM_000679 ADRA1B Homo sapiens adrenergic, alpha-1B-, receptor (ADRA1B), mRNA [NM_000679] 2.2 NM_145010 C10orf63 Homo sapiens chromosome 10 open reading frame 63 (C10orf63), mRNA [NM_145010] 2.2 NM_173798 ZCCHC12 Homo sapiens zinc finger, CCHC domain containing 12 (ZCCHC12), mRNA [NM_173798] 2.2 THC2520542 Q9XDH2_MYCTU (Q9XDH2) Proline-rich mucin homolog, partial (3%) [THC2520542] 2.2 byguest onSeptember11, 2012 NM_145273 CD300LG Homo sapiens CD300 molecule-like family member g (CD300LG), mRNA [NM_145273] 2.1 NM_000480 AMPD3 Homo sapiens adenosine monophosphate deaminase (isoform E) (AMPD3), transcript variant 3, mRNA [NM_001025390] 2.1 NM_206966 C5orf46 Homo sapiens chromosome 5 open reading frame 46 (C5orf46), mRNA [NM_206966] 2.1 NM_001629 ALOX5AP Homo sapiens arachidonate 5-lipoxygenase-activating protein (ALOX5AP), mRNA [NM_001629] 2.1 AK024243 AK024243 Homo sapiens cDNA FLJ14181 fis, clone NT2RP2004300. [AK024243] 2.1 AF085962 AF085962 Homo sapiens full length insert cDNA clone YS02G11. [AF085962] 2.1 NM_152309 PIK3AP1 Homo sapiens phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), mRNA [NM_152309] 2.1 NM_004797 ADIPOQ Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA [NM_004797] 2.1 BM511013 APBA1 ij42f09.y1 Human insulinoma Homo sapiens cDNA clone IMAGE:5633800 5', mRNA sequence [BM511013] 2.1 NM_033316 MFI2 Homo sapiens antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (MFI2), transcript variant 2, mRNA [NM_033316] 2.1 AF305836 DIO3OS Homo sapiens clone 6 DIO3AS mRNA, partial sequence; alternatively spliced. [AF469204] 2.1 NM_001010883 FAM102B Homo sapiens family with sequence similarity 102, member B (FAM102B), mRNA [NM_001010883] 2.1 BC002831 MGC4294 Homo sapiens hypothetical protein MGC4294, mRNA (cDNA clone MGC:4294 IMAGE:3636069), complete cds. [BC002831] 2.1 NR_002763 PRO0132 Homo sapiens PRO0132 protein (PRO0132), non-coding RNA [NR_002763] 2.1 NM_003155 STC1 Homo sapiens stanniocalcin 1 (STC1), mRNA [NM_003155] 2.1 NM_000153 GALC Homo sapiens galactosylceramidase (GALC), transcript variant 1, mRNA [NM_000153] 2.1 X98562 X98562 H.sapiens mRNA for dsRNA adenosine deaminase, 1D. [X98562] 2.1 AK025887 SLC2A9 Homo sapiens cDNA: FLJ22234 fis, clone HRC02022. [AK025887] 2.1 NM_014951 ZNF365 Homo sapiens zinc finger protein 365 (ZNF365), transcript variant A, mRNA [NM_014951] 2.1 AL122040 AL122040 Homo sapiens mRNA; cDNA DKFZp434G1972 (from clone DKFZp434G1972). [AL122040] 2.1 BX110856 SLC8A2 Homo sapiens solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2), mRNA [NM_015063] 2.1 NM_152775 CCDC110 Homo sapiens coiled-coil domain containing 110 (CCDC110), mRNA [NM_152775] 2.1 NM_152262 ZNF439 Homo sapiens zinc finger protein 439 (ZNF439), mRNA [NM_152262] 2.1 NM_052946 NOSTRIN Homo sapiens nitric oxide synthase trafficker (NOSTRIN), transcript variant 1, mRNA [NM_052946] 2.1 THC2678236 Q92755_HUMAN (Q92755) Transcription factor AP2 (Fragment), partial (28%) [THC2678236] 2.1 NM_019065 NECAB2 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), mRNA [NM_019065] 2.1 AK022322 UBR4 Homo sapiens cDNA FLJ12260 fis, clone MAMMA1001551. [AK022322] 2.1 AK027178 AK027178 Homo sapiens cDNA: FLJ23525 fis, clone LNG05604. [AK027178] 2.1 NM_000633 BCL2 Homo sapiens B-cell CLL/lymphoma 2 (BCL2), nuclear gene encoding mitochondrial protein, transcript variant alpha, mRNA [NM_000633] 2.1

Downloaded from NM_080385 CPA5 Homo sapiens carboxypeptidase A5 (CPA5), transcript variant 1, mRNA [NM_080385] 2.1 BC000868 MGC5590 Homo sapiens hypothetical protein MGC5590, mRNA (cDNA clone MGC:5590 IMAGE:3460233), complete cds. [BC000868] 2.1 AK024577 KBTBD2 Homo sapiens cDNA: FLJ20924 fis, clone ADSE00928. [AK024577] 2.1 NM_052934 SLC26A9 Homo sapiens solute carrier family 26, member 9 (SLC26A9), transcript variant 1, mRNA [NM_052934] 2.1 THC2668815 Q4TBH3_TETNG (Q4TBH3) Chromosome 13 SCAF7124, whole genome shotgun sequence, partial (3%) [THC2668815] 2.1 NM_144586 LYPD1 Homo sapiens LY6/PLAUR domain containing 1 (LYPD1), transcript variant 1, mRNA [NM_144586] 2.1 http://hyper.ahajournals.org/ NM_001039528 ENST00000314481 cDNA FLJ34503 fis, clone HLUNG2005738 [Source:UniProtKB/TrEMBL;Acc:Q8NAY8] [ENST00000314481] 2.1 NM_018324 OLAH Homo sapiens oleoyl-ACP hydrolase (OLAH), transcript variant 2, mRNA [NM_001039702] 2.1 RECQL Homo sapiens RecQ protein-like (DNA helicase Q1-like) (RECQL), transcript variant 1, mRNA [NM_002907] 2.1 NM_031410 PCDHA1 Homo sapiens protocadherin alpha 1 (PCDHA1), transcript variant 2, mRNA [NM_031410] 2.1 NM_018245 OGDHL Homo sapiens oxoglutarate dehydrogenase-like (OGDHL), mRNA [NM_018245] 2.1 NM_001849 COL6A2 Homo sapiens collagen, type VI, alpha 2 (COL6A2), transcript variant 2C2, mRNA [NM_001849] 2.1 NM_153456 HS6ST3 Homo sapiens heparan sulfate 6-O-sulfotransferase 3 (HS6ST3), mRNA [NM_153456] 2.1 NM_152322 BTBD11 Homo sapiens BTB (POZ) domain containing 11 (BTBD11), transcript variant a, mRNA [NM_001018072] 2.1 byguest onSeptember11, 2012 NM_020066 FMN2 Homo sapiens formin 2 (FMN2), mRNA [NM_020066] 2.1 NM_019644 ANKRD7 Homo sapiens ankyrin repeat domain 7 (ANKRD7), transcript variant 1, mRNA [NM_001077708] 2.1 NM_006941 SOX10 Homo sapiens SRY (sex determining region Y)-box 10 (SOX10), mRNA [NM_006941] 2.1 BC101436 FLJ25404 Homo sapiens hypothetical protein FLJ25404 (FLJ25404), transcript variant 2, mRNA [NM_001109660] 2.1 THC2661496 Q3C9X1_9CLOT (Q3C9X1) Cobalt transport protein, partial (5%) [THC2661496] 2.1 NM_032784 RSPO3 Homo sapiens R-spondin 3 homolog (Xenopus laevis) (RSPO3), mRNA [NM_032784] 2.1 NM_152676 FBXO15 Homo sapiens F-box protein 15 (FBXO15), transcript variant 1, mRNA [NM_152676] 2.1 NM_017563 IL17RD Homo sapiens interleukin 17 receptor D (IL17RD), mRNA [NM_017563] 2.1 CF552909 AGENCOURT_15595836 NIH_MGC_183 Homo sapiens cDNA clone IMAGE:30529642 5', mRNA sequence [CF552909] 2.1 BE221781 BE221781 BE221781 hr59b03.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:3132749 3', mRNA sequence [BE221781] 2.1 NM_032040 CCDC8 Homo sapiens coiled-coil domain containing 8 (CCDC8), mRNA [NM_032040] 2.1 NM_173160 FXYD4 Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), mRNA [NM_173160] 2.1 BG547557 BG547557 BG547557 602575410F1 NIH_MGC_77 Homo sapiens cDNA clone IMAGE:4703546 5', mRNA sequence [BG547557] 2.1 AK021734 LOC153811 Homo sapiens cDNA FLJ11672 fis, clone HEMBA1004733. [AK021734] 2.1 THC2591019 ZN566_HUMAN (Q969W8) Zinc finger protein 566, partial (48%) [THC2591019] 2.1 AK091802 SGK269 Homo sapiens NKF3 kinase family member (SGK269), mRNA [NM_024776] 2.1 NM_003408 ZFP37 Homo sapiens zinc finger protein 37 homolog (mouse) (ZFP37), mRNA [NM_003408] 2.1 NM_006072 CCL26 Homo sapiens chemokine (C-C motif) ligand 26 (CCL26), mRNA [NM_006072] 2.1 NM_017899 TESC Homo sapiens tescalcin (TESC), mRNA [NM_017899] 2.1 AA344632 THC2724046 AA344632 EST50565 Gall bladder I Homo sapiens cDNA 5' end, mRNA sequence [AA344632] 2.1 NM_033309 B3GNT9 Homo sapiens UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 9 (B3GNT9), mRNA [NM_033309] 2.1 AK098811 RFT1 Homo sapiens RFT1 homolog (S. cerevisiae) (RFT1), mRNA [NM_052859] 2.1 XM_939709 THC2730708 ZNF73_HUMAN (O43830) Zinc finger protein 73 (Zinc finger protein 186) (hZNF2), complete [THC2730708] 2.1 AL713771 NFYC Homo sapiens mRNA; cDNA DKFZp667G242 (from clone DKFZp667G242). [AL713771] 2.1 NM_003126 SPTA1 Homo sapiens spectrin, alpha, erythrocytic 1 (elliptocytosis 2) (SPTA1), mRNA [NM_003126] 2.1 AF533251 ZNF396 Homo sapiens zinc finger protein (ZNF396) mRNA, complete cds. [AF533251] 2.1 NM_001013839 EXOC7 Homo sapiens exocyst complex component 7 (EXOC7), transcript variant 1, mRNA [NM_001013839] 2.1 NM_014817 KIAA0644 Homo sapiens KIAA0644 gene product (KIAA0644), mRNA [NM_014817] 2.1 BC029783 STAT3 Homo sapiens signal transducer and activator of transcription 3 (acute-phase response factor), mRNA (cDNA clone IMAGE:5193339), with apparent retained i 2.1

Downloaded from THC2657737 NKG2D_HUMAN (P26718) NKG2-D type II integral membrane protein (NKG2-D-activating NK receptor) (NK cell receptor D) (Killer cell lectin-like receptor sub 2.1 AK054929 NCAM1 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 3, mRNA [NM_001076682] 2.1 AK090418 LOC349196 Homo sapiens mRNA for FLJ00326 protein. [AK090418] 2.1 BC016334 ZNF568 Homo sapiens zinc finger protein 568, mRNA (cDNA clone IMAGE:4052822), partial cds. [BC016334] 2.1 NM_005711 EDIL3 Homo sapiens EGF-like repeats and discoidin I-like domains 3 (EDIL3), mRNA [NM_005711] 2.1 NM_019609 CPXM1 Homo sapiens carboxypeptidase X (M14 family), member 1 (CPXM1), mRNA [NM_019609] 2.1 http://hyper.ahajournals.org/ NM_016212 TP53TG3 Homo sapiens TP53 target 3 (TP53TG3), mRNA [NM_016212] 2.1 AK096580 AK096580 Homo sapiens cDNA FLJ39261 fis, clone OCBBF2009391. [AK096580] 2.1 NM_153183 NUDT10 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 10 (NUDT10), mRNA [NM_153183] 2.1 NM_139055 ADAMTS15 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15), mRNA [NM_139055] 2.1 NM_201575 SEZ6L2 Homo sapiens seizure related 6 homolog (mouse)-like 2 (SEZ6L2), transcript variant 2, mRNA [NM_201575] 2.1 NM_173666 DTWD2 Homo sapiens DTW domain containing 2 (DTWD2), mRNA [NM_173666] 2.1 NM_005854 RAMP2 Homo sapiens receptor (G protein-coupled) activity modifying protein 2 (RAMP2), mRNA [NM_005854] 2.1 NM_014571 HEYL Homo sapiens hairy/enhancer-of-split related with YRPW motif-like (HEYL), mRNA [NM_014571] 2.1 byguest onSeptember11, 2012 NM_020130 C8orf4 Homo sapiens chromosome 8 open reading frame 4 (C8orf4), mRNA [NM_020130] 2.1 NM_005360 MAF Homo sapiens v- musculoaponeurotic fibrosarcoma oncogene homolog (avian) (MAF), transcript variant 2, mRNA [NM_001031804] 2.0 NM_018659 CYTL1 Homo sapiens cytokine-like 1 (CYTL1), mRNA [NM_018659] 2.0 NM_014861 ATP2C2 Homo sapiens ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2), mRNA [NM_014861] 2.0 NM_031302 GLT8D2 Homo sapiens glycosyltransferase 8 domain containing 2 (GLT8D2), mRNA [NM_031302] 2.0 NM_005314 GRPR Homo sapiens gastrin-releasing peptide receptor (GRPR), mRNA [NM_005314] 2.0 NM_020225 STOX2 Homo sapiens storkhead box 2 (STOX2), mRNA [NM_020225] 2.0 NM_003390 WEE1 Homo sapiens WEE1 homolog (S. pombe) (WEE1), mRNA [NM_003390] 2.0 T52140 THC2726661 T52140 yb29h11.r1 Stratagene fetal spleen (#937205) Homo sapiens cDNA clone IMAGE:72645 5', mRNA sequence [T52140] 2.0 BU629094 BU629094 BU629094 UI-H-FL0-bdh-d-02-0-UI.s1 NCI_CGAP_FL0 Homo sapiens cDNA clone UI-H-FL0-bdh-d-02-0-UI 3', mRNA sequence [BU629094] 2.0 NM_018354 C20orf46 Homo sapiens chromosome 20 open reading frame 46 (C20orf46), mRNA [NM_018354] 2.0 NM_002298 LCP1 Homo sapiens lymphocyte cytosolic protein 1 (L-plastin) (LCP1), mRNA [NM_002298] 2.0 NM_139024 TSPAN32 Homo sapiens cDNA FLJ46034 fis, clone SPLEN2033996. [AK128812] 2.0 NM_006314 CNKSR1 Homo sapiens connector enhancer of kinase suppressor of Ras 1 (CNKSR1), transcript variant 1, mRNA [NM_006314] 2.0 NM_004936 CDKN2B Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 1, mRNA [NM_004936] 2.0 NM_182511 CBLN2 Homo sapiens cerebellin 2 precursor (CBLN2), mRNA [NM_182511] 2.0 BX427588 BX427588 BX427588 Homo sapiens FETAL LIVER Homo sapiens cDNA clone CS0DM011YM01 3-PRIME, mRNA sequence [BX427588] 2.0 NM_024781 CCDC102B Homo sapiens coiled-coil domain containing 102B (CCDC102B), transcript variant 2, mRNA [NM_024781] 2.0 NM_181489 ZNF445 Homo sapiens zinc finger protein 445 (ZNF445), mRNA [NM_181489] 2.0 NM_005825 RASGRP2 Homo sapiens RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 2, mRNA [NM_153819] 2.0 BX100598 THC2689557 Y078_THEAC (Q9HLZ5) UPF0284 protein Ta0078, partial (5%) [THC2689557] 2.0 NM_001542 IGSF3 Homo sapiens immunoglobulin superfamily, member 3 (IGSF3), transcript variant 1, mRNA [NM_001542] 2.0 NM_003728 UNC5C Homo sapiens unc-5 homolog C (C. elegans) (UNC5C), mRNA [NM_003728] 2.0 NM_002839 PTPRD Homo sapiens protein tyrosine phosphatase, receptor type, D (PTPRD), transcript variant 1, mRNA [NM_002839] 2.0 NR_001443 LOC339240 Homo sapiens keratin pseudogene (LOC339240), non-coding RNA [NR_001443] 2.0 NM_000683 ADRA2C Homo sapiens adrenergic, alpha-2C-, receptor (ADRA2C), mRNA [NM_000683] 2.0 CN333321 AI424779 AI424779 tf09h08.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE:2095743 3', mRNA sequence [AI424779] 2.0 NM_153229 TMEM92 Homo sapiens transmembrane protein 92 (TMEM92), mRNA [NM_153229] 2.0 NM_006108 SPON1 Homo sapiens spondin 1, extracellular matrix protein (SPON1), mRNA [NM_006108] 2.0

Downloaded from NM_024761 MOBKL2B Homo sapiens MOB1, Mps One Binder kinase activator-like 2B (yeast) (MOBKL2B), mRNA [NM_024761] 2.0 NM_002236 KCNF1 Homo sapiens potassium voltage-gated channel, subfamily F, member 1 (KCNF1), mRNA [NM_002236] 2.0 NM_004558 NRTN Homo sapiens neurturin (NRTN), mRNA [NM_004558] 2.0 NM_032021 TMEM133 Homo sapiens transmembrane protein 133 (TMEM133), mRNA [NM_032021] 2.0 NM_001040167 LFNG Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 1, mRNA [NM_001040167] 2.0 BC047078 SUGT1L1 Homo sapiens SGT1, suppressor of G2 allele of SKP1 like 1 (S. cerevisiae) (SUGT1L1), non-coding RNA [NR_003365] 2.0 http://hyper.ahajournals.org/ NM_080429 AQP10 Homo sapiens aquaporin 10 (AQP10), mRNA [NM_080429] 2.0 NM_198721 COL25A1 Homo sapiens collagen, type XXV, alpha 1 (COL25A1), transcript variant 1, mRNA [NM_198721] 2.0 AK056190 DFNB31 Homo sapiens cDNA FLJ31628 fis, clone NT2RI2003344. [AK056190] 2.0 NM_000576 IL1B Homo sapiens interleukin 1, beta (IL1B), mRNA [NM_000576] 2.0 BC017721 LOC100144602 Homo sapiens hypothetical, mRNA (cDNA clone IMAGE:4429392), partial cds. [BC017721] 2.0 NM_015692 CPAMD8 Homo sapiens C3 and PZP-like, alpha-2-macroglobulin domain containing 8 (CPAMD8), mRNA [NM_015692] 2.0 NM_023112 OTUB2 Homo sapiens OTU domain, ubiquitin aldehyde binding 2 (OTUB2), mRNA [NM_023112] 2.0 NM_175887 PRR15 Homo sapiens proline rich 15 (PRR15), mRNA [NM_175887] 2.0 byguest onSeptember11, 2012 NM_172366 FBXO16 Homo sapiens F-box protein 16 (FBXO16), mRNA [NM_172366] 2.0 BC006011 DDI2 Homo sapiens DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae), mRNA (cDNA clone MGC:14844 IMAGE:4296688), complete cds. [BC006011] 2.0 AY335940 IRX2 Homo sapiens iroquois homeobox 2 (IRX2), transcript variant 1, mRNA [NM_033267] 2.0 BC034499 ZNF738 Homo sapiens zinc finger protein 738, mRNA (cDNA clone IMAGE:4838161), complete cds. [BC034499] 2.0 NM_145173 DIRAS1 Homo sapiens DIRAS family, GTP-binding RAS-like 1 (DIRAS1), mRNA [NM_145173] 2.0 NM_001039643 ENST00000396827 Zinc finger and SCAN domain-containing protein 12 (Zinc finger protein 96)(Zinc finger protein 305) [Source:UniProtKB/Swiss-Prot;Acc:O43309] [ENST000003 2.0 AA703432 AA703432 AA703432 zj13a09.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:450136 3' similar to gb:L07077 ENOYL-COA HYDRATASE ( 2.0 NM_000250 MPO Homo sapiens myeloperoxidase (MPO), nuclear gene encoding mitochondrial protein, mRNA [NM_000250] 2.0 NM_174901 FAM9C Homo sapiens family with sequence similarity 9, member C (FAM9C), mRNA [NM_174901] -2.0 BC008001 BC008001 Homo sapiens mRNA similar to hypothetical protein FLJ21463 (cDNA clone IMAGE:3504595). [BC008001] -2.0 NM_152426 APOBEC3F Homo sapiens cDNA FLJ60505 complete cds, highly similar to Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F. [AK294810] -2.0 NM_000819 GART Homo sapiens phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), trans -2.0 NM_203411 TMEM88 Homo sapiens transmembrane protein 88 (TMEM88), mRNA [NM_203411] -2.0 AW377662 AW377662 AW377662 PM0-CT0237-141099-001-e02 CT0237 Homo sapiens cDNA, mRNA sequence [AW377662] -2.0 NM_024032 C17orf53 Homo sapiens chromosome 17 open reading frame 53 (C17orf53), mRNA [NM_024032] -2.0 AW451975 AW451975 UI-H-BI3-alm-b-04-0-UI.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone IMAGE:2737110 3', mRNA sequence [AW451975] -2.0 NM_024574 C4orf31 Homo sapiens chromosome 4 open reading frame 31 (C4orf31), mRNA [NM_024574] -2.0 BC012317 LINCR Homo sapiens likely ortholog of mouse -inducible Neutralized-related C3HC4 RING domain protein, mRNA (cDNA clone IMAGE:3346442). [BC012317] -2.0 NM_015515 KRT23 Homo sapiens keratin 23 (histone deacetylase inducible) (KRT23), mRNA [NM_015515] -2.0 NM_005471 GNPDA1 Homo sapiens glucosamine-6-phosphate deaminase 1 (GNPDA1), mRNA [NM_005471] -2.0 NM_004751 GCNT3 Homo sapiens glucosaminyl (N-acetyl) transferase 3, mucin type (GCNT3), mRNA [NM_004751] -2.0 NM_003258 TK1 Homo sapiens thymidine kinase 1, soluble (TK1), mRNA [NM_003258] -2.0 G42982 THC2478726 Q5T697_HUMAN (Q5T697) Guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2, complete [THC2478726] -2.0 NM_007109 TCF19 Homo sapiens transcription factor 19 (SC1) (TCF19), transcript variant 1, mRNA [NM_007109] -2.0 NM_176870 MT1M Homo sapiens metallothionein 1M (MT1M), mRNA [NM_176870] -2.0 NM_002692 POLE2 Homo sapiens polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), mRNA [NM_002692] -2.0 AK001108 FLJ10246 Homo sapiens cDNA FLJ10246 fis, clone HEMBB1000673. [AK001108] -2.0 NM_030923 TMEM163 Homo sapiens transmembrane protein 163 (TMEM163), mRNA [NM_030923] -2.0 NM_145720 TIGD4 Homo sapiens tigger transposable element derived 4 (TIGD4), mRNA [NM_145720] -2.0

Downloaded from NM_002497 NEK2 Homo sapiens NIMA (never in mitosis gene a)-related kinase 2 (NEK2), mRNA [NM_002497] -2.0 NM_003335 UBA7 Homo sapiens ubiquitin-like modifier activating enzyme 7 (UBA7), mRNA [NM_003335] -2.0 NM_033647 HELB Homo sapiens helicase (DNA) B (HELB), mRNA [NM_033647] -2.0 NM_005353 ITGAD Homo sapiens integrin, alpha D (ITGAD), mRNA [NM_005353] -2.0 NM_016232 IL1RL1 Homo sapiens interleukin 1 receptor-like 1 (IL1RL1), transcript variant 1, mRNA [NM_016232] -2.0 AY271826 ZYG11A Protein zyg-11 homolog A [Source:UniProtKB/Swiss-Prot;Acc:Q6WRX3] [ENST00000371528] -2.0 http://hyper.ahajournals.org/ NM_004613 TGM2 Homo sapiens transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) (TGM2), transcript variant 1, mRNA [NM_004613] -2.0 BG030371 BG029389 602296817F1 NIH_MGC_87 Homo sapiens cDNA clone IMAGE:4390948 5', mRNA sequence [BG029389] -2.0 NM_004217 AURKB Homo sapiens aurora kinase B (AURKB), mRNA [NM_004217] -2.0 NM_000349 STAR Homo sapiens steroidogenic acute regulatory protein (STAR), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA [NM_000349] -2.0 NM_003005 SELP Homo sapiens selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), mRNA [NM_003005] -2.0 NM_053024 PFN2 Homo sapiens profilin 2 (PFN2), transcript variant 1, mRNA [NM_053024] -2.0 NM_198183 UBE2L6 Homo sapiens ubiquitin-conjugating enzyme E2L 6 (UBE2L6), transcript variant 2, mRNA [NM_198183] -2.0 NM_020929 LRRC4C Homo sapiens leucine rich repeat containing 4C (LRRC4C), mRNA [NM_020929] -2.0 byguest onSeptember11, 2012 NM_007069 PLA2G16 Homo sapiens phospholipase A2, group XVI (PLA2G16), transcript variant 1, mRNA [NM_007069] -2.0 AK090706 C14orf83 Transmembrane protein C14orf83 [Source:UniProtKB/Swiss-Prot;Acc:Q8NBD8] [ENST00000394512] -2.0 NM_153445 OR5P3 Homo sapiens , family 5, subfamily P, member 3 (OR5P3), mRNA [NM_153445] -2.0 NM_173578 ENST00000333139 Uncharacterized protein C11orf72 [Source:UniProtKB/Swiss-Prot;Acc:Q8NBR9] [ENST00000333139] -2.0 NM_002090 CXCL3 Homo sapiens chemokine (C-X-C motif) ligand 3 (CXCL3), mRNA [NM_002090] -2.1 NM_002471 MYH6 Homo sapiens myosin, heavy chain 6, cardiac muscle, alpha (MYH6), mRNA [NM_002471] -2.1 AK096570 LOC643837 Homo sapiens cDNA FLJ39251 fis, clone OCBBF2008701. [AK096570] -2.1 NM_022074 FAM111A Homo sapiens family with sequence similarity 111, member A (FAM111A), transcript variant 1, mRNA [NM_022074] -2.1 BC005407 NUP188 Homo sapiens nucleoporin 188kDa, mRNA (cDNA clone IMAGE:3461492), partial cds. [BC005407] -2.1 NM_182761 FAM170A Homo sapiens family with sequence similarity 170, member A (FAM170A), mRNA [NM_182761] -2.1 BC017357 ZNF765 Homo sapiens zinc finger protein 765, mRNA (cDNA clone IMAGE:5094445). [BC017357] -2.1 XM_376062 PAIP2B Homo sapiens poly(A) binding protein interacting protein 2B (PAIP2B), mRNA [NM_020459] -2.1 NM_005592 MUSK Homo sapiens muscle, skeletal, receptor tyrosine kinase (MUSK), mRNA [NM_005592] -2.1 NM_005330 HBE1 Homo sapiens hemoglobin, epsilon 1 (HBE1), mRNA [NM_005330] -2.1 AF086092 AF086092 Homo sapiens full length insert cDNA clone YZ87G11. [AF086092] -2.1 BC001879 BC001879 Homo sapiens, clone IMAGE:3534181, mRNA, partial cds. [BC001879] -2.1 NM_024867 SPEF2 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA [NM_024867] -2.1 AY360464 VWA3A Homo sapiens von Willebrand factor A domain containing 3A (VWA3A), mRNA [NM_173615] -2.1 NM_002620 PF4V1 Homo sapiens platelet factor 4 variant 1 (PF4V1), mRNA [NM_002620] -2.1 NM_182502 TMPRSS11B Homo sapiens transmembrane protease, serine 11B (TMPRSS11B), mRNA [NM_182502] -2.1 NM_021822 APOBEC3G Homo sapiens apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G (APOBEC3G), mRNA [NM_021822] -2.1 NM_178445 CCRL1 Homo sapiens chemokine (C-C motif) receptor-like 1 (CCRL1), transcript variant 1, mRNA [NM_178445] -2.1 NM_080818 OXGR1 Homo sapiens oxoglutarate (alpha-ketoglutarate) receptor 1 (OXGR1), mRNA [NM_080818] -2.1 NM_005505 SCARB1 Homo sapiens scavenger receptor class B, member 1 (SCARB1), transcript variant 1, mRNA [NM_005505] -2.1 NM_000450 SELE Homo sapiens selectin E (SELE), mRNA [NM_000450] -2.1 NM_005602 CLDN11 Homo sapiens claudin 11 (CLDN11), mRNA [NM_005602] -2.1 AK097045 AK097045 Homo sapiens cDNA FLJ39726 fis, clone SMINT2015306. [AK097045] -2.1 NM_147196 TMIE Homo sapiens transmembrane inner ear (TMIE), mRNA [NM_147196] -2.1 NM_002202 ISL1 Homo sapiens ISL LIM homeobox 1 (ISL1), mRNA [NM_002202] -2.1

Downloaded from NM_014788 TRIM14 Homo sapiens tripartite motif-containing 14 (TRIM14), transcript variant 1, mRNA [NM_014788] -2.1 NM_017594 DIRAS2 Homo sapiens DIRAS family, GTP-binding RAS-like 2 (DIRAS2), mRNA [NM_017594] -2.1 NM_201629 TJP2 Homo sapiens tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 2, mRNA [NM_201629] -2.1 AA724922 BC032716 Homo sapiens cDNA clone IMAGE:5518697, **** WARNING: chimeric clone ****. [BC032716] -2.1 NM_016292 TRAP1 Homo sapiens TNF receptor-associated protein 1 (TRAP1), mRNA [NM_016292] -2.1 NM_000903 NQO1 Homo sapiens NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 1, mRNA [NM_000903] -2.1 http://hyper.ahajournals.org/ BF959143 BF959143 BF959143 QV2-NN0045-011200-505-h04 NN0045 Homo sapiens cDNA, mRNA sequence [BF959143] -2.1 X98261 ZWINTAS H.sapiens mRNA for M-phase phosphoprotein, mpp5. [X98261] -2.1 AK075271 SPTLC3 Homo sapiens serine palmitoyltransferase, long chain base subunit 3 (SPTLC3), mRNA [NM_018327] -2.1 BC022083 BC022083 Homo sapiens cDNA clone IMAGE:4825558. [BC022083] -2.1 AW802986 THC2634909 AW802986 IL2-UM0077-270300-060-E04 UM0077 Homo sapiens cDNA, mRNA sequence [AW802986] -2.1 ENST00000302942 Nuclear cap-binding protein subunit 2-like [Source:UniProtKB/Swiss-Prot;Acc:A6PVI3] [ENST00000302942] -2.1 AI022779 AI022779 AI022779 ow99c06.x1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:1654954 3' similar to contains Alu repetitive element;, mRNA -2.1 NM_002098 GUCA1B Homo sapiens guanylate cyclase activator 1B (retina) (GUCA1B), mRNA [NM_002098] -2.1 byguest onSeptember11, 2012 NM_174959 SVOPL Homo sapiens SVOP-like (SVOPL), transcript variant 2, mRNA [NM_174959] -2.1 AK021897 AK021897 Homo sapiens cDNA FLJ11835 fis, clone HEMBA1006595. [AK021897] -2.1 NM_001836 CMA1 Homo sapiens chymase 1, mast cell (CMA1), mRNA [NM_001836] -2.1 CR590862 GLT8D3 Homo sapiens glycosyltransferase 8 domain containing 3 (GLT8D3), transcript variant 1, mRNA [NM_173601] -2.1 NR_002810 SOX2OT Homo sapiens overlapping transcript (non-protein coding) (SOX2OT), non-coding RNA [NR_004053] -2.1 AF150882 SCN11A Homo sapiens voltage-gated sodium channel alpha subunit, alternate splice variant SCN12A-s (SCN12A) mRNA, complete cds. [AF150882] -2.1 NM_001040078 LGALS9C Homo sapiens lectin, galactoside-binding, soluble, 9C (LGALS9C), mRNA [NM_001040078] -2.1 NM_002373 MAP1A Homo sapiens microtubule-associated protein 1A (MAP1A), mRNA [NM_002373] -2.1 NM_018248 NEIL3 Homo sapiens nei endonuclease VIII-like 3 (E. coli) (NEIL3), mRNA [NM_018248] -2.1 NM_004900 APOBEC3B Homo sapiens apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B), mRNA [NM_004900] -2.1 NM_152866 MS4A1 Homo sapiens membrane-spanning 4-domains, subfamily A, member 1 (MS4A1), transcript variant 1, mRNA [NM_152866] -2.1 NM_201631 TGM5 Homo sapiens transglutaminase 5 (TGM5), transcript variant 1, mRNA [NM_201631] -2.1 NM_004153 ORC1L Homo sapiens origin recognition complex, subunit 1-like (yeast) (ORC1L), mRNA [NM_004153] -2.1 NM_206862 TACC2 Homo sapiens transforming, acidic coiled-coil containing protein 2 (TACC2), transcript variant 1, mRNA [NM_206862] -2.1 NM_032818 C9orf100 Homo sapiens chromosome 9 open reading frame 100 (C9orf100), mRNA [NM_032818] -2.1 BC017904 BC017904 Homo sapiens, clone IMAGE:4272979, mRNA, partial cds. [BC017904] -2.1 BC030533 ENST00000390352 T-cell receptor beta V gene segment [Source:IMGT/GENE-DB;Acc:TRBV5-1] [ENST00000390352] -2.1 BX118690 BX118690 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGp998N143105, mRNA sequence [BX118690] -2.1 NM_207173 NPSR1 Homo sapiens 1 (NPSR1), transcript variant 2, mRNA [NM_207173] -2.1 NM_020770 CGN Homo sapiens cingulin (CGN), mRNA [NM_020770] -2.1 NM_001007089 RESP18 Homo sapiens regulated endocrine-specific protein 18 isoform C mRNA, complete cds. [DQ227570] -2.1 AF305815 PIGT Homo sapiens PRO0433 mRNA, complete cds. [AF305815] -2.1 NM_207006 FAM83A Homo sapiens family with sequence similarity 83, member A (FAM83A), transcript variant 2, mRNA [NM_207006] -2.1 BC008248 ENST00000397549 LOC651900 protein (HCG1987623) Fragment [Source:UniProtKB/TrEMBL;Acc:Q96JS6] [ENST00000397549] -2.1 NM_004668 MGAM Homo sapiens maltase-glucoamylase (alpha-glucosidase) (MGAM), mRNA [NM_004668] -2.1 NM_006829 C10orf116 Homo sapiens chromosome 10 open reading frame 116 (C10orf116), mRNA [NM_006829] -2.1 AK128769 CACNB2 Homo sapiens cDNA FLJ45229 fis, clone BRCAN2020972. [AK128769] -2.1 XM_167254 NP107054 GB -2.1 NM_003251 THRSP Homo sapiens thyroid hormone responsive (SPOT14 homolog, rat) (THRSP), mRNA [NM_003251] -2.2

Downloaded from AA180985 AA180985 AA180985 zp44c07.s1 Stratagene muscle 937209 Homo sapiens cDNA clone IMAGE:612300 3', mRNA sequence [AA180985] -2.2 XM_378080 LOC402377 PREDICTED: Homo sapiens hypothetical LOC402377 (LOC402377), mRNA [XM_001713941] -2.2 NM_005557 KRT16 Homo sapiens keratin 16 (KRT16), mRNA [NM_005557] -2.2 NM_024955 FOXRED2 Homo sapiens FAD-dependent oxidoreductase domain containing 2 (FOXRED2), transcript variant 1, mRNA [NM_024955] -2.2 NM_145205 HMGB4 Homo sapiens high-mobility group box 4 (HMGB4), transcript variant 1, mRNA [NM_145205] -2.2 LOC100134400 PREDICTED: Homo sapiens hypothetical protein LOC100134400 (LOC100134400), partial mRNA [XM_001718660] -2.2 http://hyper.ahajournals.org/ AL833334 LOC283278 Homo sapiens mRNA; cDNA DKFZp686B1134 (from clone DKFZp686B1134). [AL833334] -2.2 NM_054110 GALNTL2 Homo sapiens UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 (GALNTL2), mRNA [NM_054110] -2.2 NM_052909 PLEKHG4B Homo sapiens pleckstrin homology domain containing, family G (with RhoGef domain) member 4B (PLEKHG4B), mRNA [NM_052909] -2.2 AA576497 AA576497 AA576497 nm76b07.s1 NCI_CGAP_Co9 Homo sapiens cDNA clone IMAGE:1074133 3' similar to gb:M96956 EPIDERMAL GROWTH FACTOR-LIKE CRIPT -2.2 NM_001562 IL18 Homo sapiens interleukin 18 (interferon-gamma-inducing factor) (IL18), mRNA [NM_001562] -2.2 NM_012131 CLDN17 Homo sapiens claudin 17 (CLDN17), mRNA [NM_012131] -2.2 THC2627335 2F2S_A Chain A, Human Mitochondrial Acetoacetyl-Coa Thiolase. {Homo sapiens} (exp=-1; wgp=0; cg=0), partial (15%) [THC2627335] -2.2 AL137368 C6orf112 Homo sapiens mRNA; cDNA DKFZp434F2226 (from clone DKFZp434F2226). [AL137368] -2.2 byguest onSeptember11, 2012 NM_022371 TOR3A Homo sapiens torsin family 3, member A (TOR3A), mRNA [NM_022371] -2.2 NM_030642 APOL5 Homo sapiens apolipoprotein L, 5 (APOL5), mRNA [NM_030642] -2.2 G30702 AF026246 Homo sapiens mRNA containing human endogenous retrovirus H and human endogenous retrovirus E sequences. [AF026246] -2.2 NM_005092 TNFSF18 Homo sapiens tumor necrosis factor (ligand) superfamily, member 18 (TNFSF18), mRNA [NM_005092] -2.2 NM_032945 TNFRSF6B Homo sapiens tumor necrosis factor receptor superfamily, member 6b, decoy (TNFRSF6B), transcript variant M68C, mRNA [NM_032945] -2.2 NM_006231 POLE Homo sapiens polymerase (DNA directed), epsilon (POLE), mRNA [NM_006231] -2.2 NM_199254 TPTE2 Homo sapiens transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 (TPTE2), transcript variant 3, mRNA [NM_199254] -2.2 BX640843 LOC344887 Homo sapiens mRNA; cDNA DKFZp686B14224 (from clone DKFZp686B14224). [BX640843] -2.2 NM_021995 UTS2 Homo sapiens urotensin 2 (UTS2), transcript variant 1, mRNA [NM_021995] -2.2 NM_001013698 C12orf69 Homo sapiens chromosome 12 open reading frame 69 (C12orf69), mRNA [NM_001013698] -2.2 NM_001010940 C9orf135 Homo sapiens chromosome 9 open reading frame 135 (C9orf135), mRNA [NM_001010940] -2.2 BG202127 RST21481 Athersys RAGE Library Homo sapiens cDNA, mRNA sequence [BG202127] -2.2 AJ305326 CD177 Homo sapiens CD177 molecule (CD177), mRNA [NM_020406] -2.2 NM_030641 APOL6 Homo sapiens apolipoprotein L, 6 (APOL6), mRNA [NM_030641] -2.2 BX414807 BX414807 BX414807 BX414807 Homo sapiens THYMUS Homo sapiens cDNA clone CS0CAP002YP17 3-PRIME, mRNA sequence [BX414807] -2.2 AF361250 AF361250 Homo sapiens pregnancy-induced hypertension syndrome-related protein 4 (PIH4) mRNA, partial cds. [AF361250] -2.2 NM_033641 COL4A6 Homo sapiens collagen, type IV, alpha 6 (COL4A6), transcript variant B, mRNA [NM_033641] -2.2 BX101711 BX101711 NCI_CGAP_Br2 Homo sapiens cDNA clone IMAGp998H224155, mRNA sequence [BX101711] -2.2 NM_022168 IFIH1 Homo sapiens interferon induced with helicase C domain 1 (IFIH1), mRNA [NM_022168] -2.2 AJ710595 THC2505640 AJ710595 AJ710595 CMPD01 Homo sapiens cDNA clone CMPD07455, mRNA sequence [AJ710595] -2.2 R60067 R60067 R60067 yh12h06.s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE:43137 3', mRNA sequence [R60067] -2.2 AK097604 LOC100130285 Homo sapiens cDNA FLJ40285 fis, clone TESTI2027820. [AK097604] -2.2 NM_138983 OLIG1 Homo sapiens oligodendrocyte transcription factor 1 (OLIG1), mRNA [NM_138983] -2.2 NM_018058 CRTAC1 Homo sapiens cartilage acidic protein 1 (CRTAC1), mRNA [NM_018058] -2.2 NR_002949 NUDT16P Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 16 pseudogene (NUDT16P), non-coding RNA [NR_002949] -2.2 BC033117 ENST00000397186 LOC645249 protein (HCG1999295, isoform CRA_b) Fragment [Source:UniProtKB/TrEMBL;Acc:Q8TBC6] [ENST00000397186] -2.2 BC033184 BC033184 Homo sapiens cDNA clone IMAGE:4589035. [BC033184] -2.2 NM_006271 S100A1 Homo sapiens S100 calcium binding protein A1 (S100A1), mRNA [NM_006271] -2.2 AU148042 AU148042 AU148042 MAMMA1 Homo sapiens cDNA clone MAMMA1002434 3', mRNA sequence [AU148042] -2.2

Downloaded from NM_198129 LAMA3 Homo sapiens laminin, alpha 3 (LAMA3), transcript variant 1, mRNA [NM_198129] -2.2 NM_015082 FSTL4 Homo sapiens follistatin-like 4 (FSTL4), mRNA [NM_015082] -2.2 AF007190 AF007190 Homo sapiens SIB 227C intestinal mucin (MUC3) mRNA, partial cds. [AF007190] -2.2 NM_012476 VAX2 Homo sapiens ventral anterior homeobox 2 (VAX2), mRNA [NM_012476] -2.2 AK093927 LOC100128003 Homo sapiens cDNA FLJ36608 fis, clone TRACH2015824. [AK093927] -2.2 AA085955 AA085955 AA085955 zl83f11.s1 Stratagene colon (#937204) Homo sapiens cDNA clone IMAGE:511245 3', mRNA sequence [AA085955] -2.2 http://hyper.ahajournals.org/ AJ488202 NAV2 Homo sapiens partial mRNA for steerin3 protein (STEERIN3 gene), alternative exon 1b. [AJ488202] -2.2 NM_003806 HRK Homo sapiens harakiri, BCL2 interacting protein (contains only BH3 domain) (HRK), mRNA [NM_003806] -2.2 NM_006820 IFI44L Homo sapiens interferon-induced protein 44-like (IFI44L), mRNA [NM_006820] -2.2 NM_003004 SECTM1 Homo sapiens secreted and transmembrane 1 (SECTM1), mRNA [NM_003004] -2.2 AF035020 AF035020 Homo sapiens clone B2E9D5H myosin-reactive immunoglobulin heavy chain variable region mRNA, partial cds. [AF035020] -2.2 NM_001211 BUB1B Homo sapiens budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B), mRNA [NM_001211] -2.2 AK022155 AK022155 Homo sapiens cDNA FLJ12093 fis, clone HEMBB1002603. [AK022155] -2.2 NM_004615 TSPAN7 Homo sapiens tetraspanin 7 (TSPAN7), mRNA [NM_004615] -2.2 byguest onSeptember11, 2012 BC001742 LOC90834 Homo sapiens, clone IMAGE:3535910, mRNA, partial cds. [BC001742] -2.2 NM_152733 BTBD9 Homo sapiens BTB (POZ) domain containing 9 (BTBD9), transcript variant 3, mRNA [NM_152733] -2.2 NM_080664 C14orf126 Homo sapiens chromosome 14 open reading frame 126 (C14orf126), mRNA [NM_080664] -2.2 AK056855 AK056855 Homo sapiens cDNA FLJ32293 fis, clone PROST2001739. [AK056855] -2.2 NM_013994 DDR1 Homo sapiens discoidin domain receptor tyrosine kinase 1 (DDR1), transcript variant 1, mRNA [NM_013993] -2.2 NM_145641 APOL3 Homo sapiens apolipoprotein L, 3 (APOL3), transcript variant beta/a, mRNA [NM_145641] -2.2 NM_199180 KIRREL2 Homo sapiens kin of IRRE like 2 (Drosophila) (KIRREL2), transcript variant 3, mRNA [NM_199180] -2.2 NM_005821 NBR2 Homo sapiens neighbor of BRCA1 gene 2 (NBR2), non-coding RNA [NR_003108] -2.2 NM_152284 CHMP4C Homo sapiens chromatin modifying protein 4C (CHMP4C), mRNA [NM_152284] -2.3 NM_016190 CRNN Homo sapiens cornulin (CRNN), mRNA [NM_016190] -2.3 BC047704 SPATA3 Homo sapiens spermatogenesis associated 3, mRNA (cDNA clone IMAGE:5744971). [BC047704] -2.3 NM_001075 UGT2B10 Homo sapiens UDP glucuronosyltransferase 2 family, polypeptide B10 (UGT2B10), mRNA [NM_001075] -2.3 NM_006187 OAS3 Homo sapiens 2'-5'-oligoadenylate synthetase 3, 100kDa (OAS3), mRNA [NM_006187] -2.3 NM_033254 BOC Homo sapiens Boc homolog (mouse) (BOC), mRNA [NM_033254] -2.3 NM_024895 PDZD7 Homo sapiens PDZ domain containing 7 (PDZD7), mRNA [NM_024895] -2.3 NM_004079 CTSS Homo sapiens cathepsin S (CTSS), mRNA [NM_004079] -2.3 NM_002171 IFNA10 Homo sapiens interferon, alpha 10 (IFNA10), mRNA [NM_002171] -2.3 NM_013268 LGALS13 Homo sapiens lectin, galactoside-binding, soluble, 13 (LGALS13), mRNA [NM_013268] -2.3 NM_002764 PRPS1 Homo sapiens phosphoribosyl pyrophosphate synthetase 1 (PRPS1), mRNA [NM_002764] -2.3 AI199605 AI199605 AI199605 qi59f07.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE:1860805 3' similar to gb:M59849 FIBRILLARIN (HUMAN);, mRNA sequence [AI19 -2.3 AK092432 PSCA Homo sapiens cDNA FLJ35113 fis, clone PLACE6007242. [AK092432] -2.3 NM_003706 PLA2G4C Homo sapiens phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), mRNA [NM_003706] -2.3 NM_003868 FGF16 Homo sapiens fibroblast growth factor 16 (FGF16), mRNA [NM_003868] -2.3 BX093444 BX093444 NCI_CGAP_Kid5 Homo sapiens cDNA clone IMAGp998I213986 ; IMAGE:1572956, mRNA sequence [BX093444] -2.3 NM_002982 CCL2 Homo sapiens chemokine (C-C motif) ligand 2 (CCL2), mRNA [NM_002982] -2.3 CK825147 THC2654127 XKR4_MOUSE (Q5GH67) XK-related protein 4, partial (3%) [THC2654127] -2.3 AK091289 ZNF367 Homo sapiens zinc finger protein 367 (ZNF367), mRNA [NM_153695] -2.3 AI475779 AI475779 AI475779 tc94c07.x1 NCI_CGAP_CLL1 Homo sapiens cDNA clone IMAGE:2073804 3' similar to contains Alu repetitive element;contains element MER22 rep -2.3 NM_178460 SIRPD Homo sapiens signal-regulatory protein delta (SIRPD), mRNA [NM_178460] -2.3

Downloaded from AK058001 DENND3 Homo sapiens cDNA FLJ25272 fis, clone STM05694. [AK058001] -2.3 NM_001003665 C1orf95 Homo sapiens chromosome 1 open reading frame 95 (C1orf95), mRNA [NM_001003665] -2.3 NM_080869 WFDC12 Homo sapiens WAP four-disulfide core domain 12 (WFDC12), mRNA [NM_080869] -2.3 CB052856 CB052856 CB052856 NISC_gl10e12.x1 NCI_CGAP_Lei2 Homo sapiens cDNA clone IMAGE:3290591 3', mRNA sequence [CB052856] -2.3 AK026422 AK026422 Homo sapiens cDNA: FLJ22769 fis, clone KAIA1316. [AK026422] -2.3 NM_009587 LGALS9 Homo sapiens lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 1, mRNA [NM_009587] -2.3 http://hyper.ahajournals.org/ AK058068 KLKBL4 Homo sapiens plasma kallikrein-like protein 4 (KLKBL4), mRNA [NM_001080492] -2.3 NM_002637 PHKA1 Homo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 1, mRNA [NM_002637] -2.3 D86980 TTC9 Homo sapiens tetratricopeptide repeat domain 9 (TTC9), mRNA [NM_015351] -2.3 NM_000587 C7 Homo sapiens complement component 7 (C7), mRNA [NM_000587] -2.3 NM_014935 PLEKHA6 Homo sapiens pleckstrin homology domain containing, family A member 6 (PLEKHA6), mRNA [NM_014935] -2.4 AI138440 AI138440 qc53a11.x1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGE:1713308 3', mRNA sequence [AI138440] -2.4 NM_175882 IMP5 Homo sapiens intramembrane protease 5 (IMP5), mRNA [NM_175882] -2.4 NM_004456 EZH2 Homo sapiens enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, mRNA [NM_004456] -2.4 byguest onSeptember11, 2012 AK001857 HEATR1 Homo sapiens cDNA FLJ10995 fis, clone PLACE1002170. [AK001857] -2.4 NM_033184 KRTAP2-4 Homo sapiens keratin associated protein 2-4 (KRTAP2-4), mRNA [NM_033184] -2.4 NM_007048 BTN3A1 Homo sapiens butyrophilin, subfamily 3, member A1 (BTN3A1), mRNA [NM_007048] -2.4 AF170307 ENST00000375268 NAG11 [Source:UniProtKB/TrEMBL;Acc:Q8WZ93] [ENST00000375268] -2.4 NM_198081 ENST00000369021 Sex comb on midleg-like protein 4 [Source:UniProtKB/Swiss-Prot;Acc:Q8N228] [ENST00000369021] -2.4 NM_000866 HTR1F Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1F (HTR1F), mRNA [NM_000866] -2.4 AI950749 AI950749 AI950749 wx54f02.x1 NCI_CGAP_Lu28 Homo sapiens cDNA clone IMAGE:2547483 3', mRNA sequence [AI950749] -2.4 NM_001040179 MCHR2 Homo sapiens melanin-concentrating hormone receptor 2 (MCHR2), transcript variant 1, mRNA [NM_001040179] -2.4 NM_024512 LRRC2 Homo sapiens leucine rich repeat containing 2 (LRRC2), transcript variant 1, mRNA [NM_024512] -2.4 NM_000236 LIPC Homo sapiens lipase, hepatic (LIPC), mRNA [NM_000236] -2.4 NM_133509 RAD51L1 Homo sapiens RAD51-like 1 (S. cerevisiae) (RAD51L1), transcript variant 3, mRNA [NM_133509] -2.4 BC038512 BC038512 Homo sapiens cDNA clone IMAGE:5262734. [BC038512] -2.4 NM_017554 PARP14 Homo sapiens poly (ADP-ribose) polymerase family, member 14 (PARP14), mRNA [NM_017554] -2.4 NM_178822 IGSF10 Homo sapiens immunoglobulin superfamily, member 10 (IGSF10), mRNA [NM_178822] -2.4 AI028577 AI028577 AI028577 ow01h11.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1645605 3' similar to gb:K00558 TUBULIN ALPHA-1 CHAIN (HUMAN);, mRNA -2.4 BC033582 BC033582 Homo sapiens mRNA similar to LOC166173 (cDNA clone IMAGE:5203730). [BC033582] -2.4 NM_000073 CD3G Homo sapiens CD3g molecule, gamma (CD3-TCR complex) (CD3G), mRNA [NM_000073] -2.4 NM_032880 IGSF21 Homo sapiens immunoglobin superfamily, member 21 (IGSF21), mRNA [NM_032880] -2.4 AK056827 AK056827 Homo sapiens cDNA FLJ32265 fis, clone PROST1000412. [AK056827] -2.4 THC2754005 ALU4_HUMAN (P39191) Alu subfamily SB2 sequence contamination warning entry, partial (10%) [THC2754005] -2.4 NM_018661 DEFB103A Homo sapiens defensin, beta 103A (DEFB103A), mRNA [NM_018661] -2.4 AF161364 AF161364 Homo sapiens HSPC101 mRNA, partial cds. [AF161364] -2.4 NM_000063 C2 Homo sapiens complement component 2 (C2), mRNA [NM_000063] -2.4 AB037785 MICAL3 Homo sapiens microtubule associated monoxygenase, calponin and LIM domain containing 3, mRNA (cDNA clone IMAGE:5540180). [BC064400] -2.4 AB209275 MAN1C1 Homo sapiens mRNA for mannosidase, alpha, class 1C, member 1 variant protein. [AB209275] -2.5 NM_001018036 TSHR Homo sapiens thyroid stimulating hormone receptor (TSHR), transcript variant 2, mRNA [NM_001018036] -2.5 NM_030624 KLHL15 Homo sapiens kelch-like 15 (Drosophila) (KLHL15), mRNA [NM_030624] -2.5 NM_004159 PSMB8 Homo sapiens proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) (PSMB8), transcript variant 1, mRNA [NM_004159] -2.5 NM_017527 LY6K Homo sapiens lymphocyte antigen 6 complex, locus K (LY6K), mRNA [NM_017527] -2.5

Downloaded from NM_020208 SLC6A20 Homo sapiens solute carrier family 6 (proline IMINO transporter), member 20 (SLC6A20), transcript variant 1, mRNA [NM_020208] -2.5 DNM1P31 PREDICTED: Homo sapiens similar to hCG1743199 (LOC644153), mRNA [XM_001717551] -2.5 NM_182536 GKN2 Homo sapiens gastrokine 2 (GKN2), mRNA [NM_182536] -2.5 THC2682319 Q5U623_HUMAN (Q5U623) Activating transcription factor 7 interacting protein 2 (MBD1-containing chromatin associated factor 2), partial (3%) [THC2682319] -2.5 NM_001778 CD48 Homo sapiens CD48 molecule (CD48), mRNA [NM_001778] -2.5 AB058689 TMEM132B Homo sapiens mRNA for KIAA1786 protein, partial cds. [AB058689] -2.5 http://hyper.ahajournals.org/ NM_052882 ZIM3 Homo sapiens zinc finger, imprinted 3 (ZIM3), mRNA [NM_052882] -2.5 X77737 SLC4A1 Homo sapiens solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) (SLC4A1), mRNA [NM_000342] -2.5 NM_018996 TNRC6C Homo sapiens trinucleotide repeat containing 6C (TNRC6C), transcript variant 2, mRNA [NM_018996] -2.5 NM_001014435 CA7 Homo sapiens carbonic anhydrase VII (CA7), transcript variant 2, mRNA [NM_001014435] -2.5 NM_005108 XYLB Homo sapiens xylulokinase homolog (H. influenzae) (XYLB), mRNA [NM_005108] -2.5 NM_000201 ICAM1 Homo sapiens intercellular adhesion molecule 1 (ICAM1), mRNA [NM_000201] -2.5 AK056135 DUXAP10 Homo sapiens cDNA FLJ31573 fis, clone NT2RI2001769. [AK056135] -2.5 NM_005764 PDZK1IP1 Homo sapiens PDZK1 interacting protein 1 (PDZK1IP1), mRNA [NM_005764] -2.5 byguest onSeptember11, 2012 NM_001005359 POTEE Homo sapiens POTE ankyrin domain family, member E (POTEE), mRNA [NM_001083538] -2.5 NM_001898 CST1 Homo sapiens cystatin SN (CST1), mRNA [NM_001898] -2.5 NM_001165 BIRC3 Homo sapiens baculoviral IAP repeat-containing 3 (BIRC3), transcript variant 1, mRNA [NM_001165] -2.5 XM_941503 LOC652159 Unknown -2.5 AF427489 ENST00000324988 Uncharacterized protein C21orf94 [Source:UniProtKB/Swiss-Prot;Acc:P59092] [ENST00000324988] -2.5 NM_032744 C6orf105 Homo sapiens chromosome 6 open reading frame 105 (C6orf105), mRNA [NM_032744] -2.5 NM_022161 BIRC7 Homo sapiens baculoviral IAP repeat-containing 7 (BIRC7), transcript variant 2, mRNA [NM_022161] -2.5 NM_153015 TMEM74 Homo sapiens transmembrane protein 74 (TMEM74), mRNA [NM_153015] -2.5 BX099599 LOC727992 BX099599 Soares_testis_NHT Homo sapiens cDNA clone IMAGp998H173559, mRNA sequence [BX099599] -2.5 NM_014383 ZBTB32 Homo sapiens zinc finger and BTB domain containing 32 (ZBTB32), mRNA [NM_014383] -2.5 NM_001739 CA5A Homo sapiens carbonic anhydrase VA, mitochondrial (CA5A), nuclear gene encoding mitochondrial protein, mRNA [NM_001739] -2.5 NM_033364 C3orf15 Homo sapiens chromosome 3 open reading frame 15 (C3orf15), mRNA [NM_033364] -2.6 NM_017821 RHBDL2 Homo sapiens rhomboid, veinlet-like 2 (Drosophila) (RHBDL2), mRNA [NM_017821] -2.6 NM_002089 CXCL2 Homo sapiens chemokine (C-X-C motif) ligand 2 (CXCL2), mRNA [NM_002089] -2.6 XM_937549 LOC100132738 PREDICTED: Homo sapiens similar to hCG1817845 (LOC100132738), mRNA [XM_001714359] -2.6 NM_001008708 CHAC2 Homo sapiens ChaC, cation transport regulator homolog 2 (E. coli) (CHAC2), mRNA [NM_001008708] -2.6 NM_022820 CYP3A43 Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 43 (CYP3A43), transcript variant 1, mRNA [NM_022820] -2.6 AK026266 FER1L4 Homo sapiens fer-1-like 4 (C. elegans) (FER1L4), non-coding RNA [NR_024377] -2.6 NM_022147 RTP4 Homo sapiens receptor (chemosensory) transporter protein 4 (RTP4), mRNA [NM_022147] -2.6 NM_178161 PTF1A Homo sapiens pancreas specific transcription factor, 1a (PTF1A), mRNA [NM_178161] -2.6 NM_014264 PLK4 Homo sapiens polo-like kinase 4 (Drosophila) (PLK4), mRNA [NM_014264] -2.6 T12590 T12590 T12590 CHR90110 Chromosome 9 exon II Homo sapiens cDNA clone P94_55 5' and 3', mRNA sequence [T12590] -2.6 NM_003625 PPFIA2 Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), mRNA [NM_003625] -2.6 THC2633633 Q8XNF6_CLOPE (Q8XNF6) Probable transcriptional regulator, partial (5%) [THC2633633] -2.6 NM_170697 ALDH1A2 Homo sapiens aldehyde dehydrogenase 1 family, member A2 (ALDH1A2), transcript variant 3, mRNA [NM_170697] -2.7 AF113009 AF113009 Homo sapiens PRO0297 mRNA, complete cds. [AF113009] -2.7 NM_018402 IL26 Homo sapiens interleukin 26 (IL26), mRNA [NM_018402] -2.7 BC012036 BC012036 Homo sapiens cDNA clone MGC:21733 IMAGE:4517792, complete cds. [BC012036] -2.7 NM_014644 PDE4DIP Homo sapiens phosphodiesterase 4D interacting protein (PDE4DIP), transcript variant 1, mRNA [NM_014644] -2.7

Downloaded from AY358224 UNQ9368 Homo sapiens RTFV9368 (SLED1), non-coding RNA [NR_003542] -2.7 NM_006781 C6orf10 Homo sapiens chromosome 6 open reading frame 10 (C6orf10), mRNA [NM_006781] -2.7 NM_172069 PLEKHH2 Homo sapiens pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 (PLEKHH2), mRNA [NM_172069] -2.7 NM_006252 PRKAA2 Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2), mRNA [NM_006252] -2.7 AK026467 CD22 Homo sapiens cDNA: FLJ22814 fis, clone KAIA3004. [AK026467] -2.7 NM_003008 SEMG2 Homo sapiens semenogelin II (SEMG2), mRNA [NM_003008] -2.7 http://hyper.ahajournals.org/ NM_003265 TLR3 Homo sapiens toll-like receptor 3 (TLR3), mRNA [NM_003265] -2.7 NM_001032389 LOC389517 Homo sapiens Williams Beuren syndrome chromosome region 19 pseudogene (LOC389517), non-coding RNA [NR_003664] -2.7 NM_016830 VAMP1 Homo sapiens vesicle-associated membrane protein 1 (synaptobrevin 1) (VAMP1), transcript variant 3, mRNA [NM_016830] -2.7 NM_000087 CNGA1 Homo sapiens cyclic nucleotide gated channel alpha 1 (CNGA1), transcript variant 2, mRNA [NM_000087] -2.8 NM_033084 FANCD2 Homo sapiens Fanconi anemia, complementation group D2 (FANCD2), transcript variant 1, mRNA [NM_033084] -2.8 NM_130797 DPP6 Homo sapiens dipeptidyl-peptidase 6 (DPP6), transcript variant 3, mRNA [NM_001039350] -2.8 NM_004732 KCNAB3 Homo sapiens potassium voltage-gated channel, shaker-related subfamily, beta member 3 (KCNAB3), mRNA [NM_004732] -2.8 NM_033292 CASP1 Homo sapiens caspase 1, apoptosis-related peptidase (interleukin 1, beta, convertase) (CASP1), transcript variant alpha, mRNA [NM_033292] -2.8 byguest onSeptember11, 2012 NM_001030005 CPLX3 Homo sapiens complexin 3 (CPLX3), mRNA [NM_001030005] -2.8 AI911586 AI911586 ty73h01.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2284753 3' similar to SW:TTL_PIG P38160 TUBULIN--TYROSINE LIGASE ;, mRNA seque -2.8 NM_052917 GALNT13 Homo sapiens UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13 (GalNAc-T13) (GALNT13), mRNA [NM_052917] -2.8 NM_020203 MEPE Homo sapiens matrix extracellular phosphoglycoprotein (MEPE), mRNA [NM_020203] -2.8 NM_001015038 PAGE2B Homo sapiens P antigen family, member 2B (PAGE2B), mRNA [NM_001015038] -2.8 NM_004355 CD74 Homo sapiens CD74 molecule, major histocompatibility complex, class II invariant chain (CD74), transcript variant 3, mRNA [NM_001025158] -2.8 AK093065 MUC19 Homo sapiens cDNA FLJ35746 fis, clone TESTI2004399, moderately similar to APOMUCIN. [AK093065] -2.9 AB032526 AB032526 Homo sapiens NKCC2 mRNA for Na-K-2Cl cotransporter isoform III, partial cds. [AB032526] -2.9 KIAA1751 Homo sapiens KIAA1751 (KIAA1751), mRNA [NM_001080484] -2.9 NM_019885 CYP26B1 Homo sapiens cytochrome P450, family 26, subfamily B, polypeptide 1 (CYP26B1), mRNA [NM_019885] -2.9 AB051441 KIAA1654 Homo sapiens mRNA for KIAA1654 protein, partial cds. [AB051441] -2.9 NM_016582 SLC15A3 Homo sapiens solute carrier family 15, member 3 (SLC15A3), mRNA [NM_016582] -2.9 NM_032445 MEGF11 Homo sapiens multiple EGF-like-domains 11 (MEGF11), mRNA [NM_032445] -2.9 NM_001008494 ISX Homo sapiens intestine-specific homeobox (ISX), mRNA [NM_001008494] -2.9 NM_203380 ACSL5 Homo sapiens acyl-CoA synthetase long-chain family member 5 (ACSL5), transcript variant 3, mRNA [NM_203380] -2.9 NM_014985 CEP152 Homo sapiens centrosomal protein 152kDa (CEP152), mRNA [NM_014985] -2.9 NM_207372 SH2D4B Homo sapiens SH2 domain containing 4B (SH2D4B), mRNA [NM_207372] -3.0 NM_024012 HTR5A Homo sapiens 5-hydroxytryptamine (serotonin) receptor 5A (HTR5A), mRNA [NM_024012] -3.0 NM_014787 DNAJC6 Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 6 (DNAJC6), mRNA [NM_014787] -3.0 NM_144648 LRGUK Homo sapiens leucine-rich repeats and guanylate kinase domain containing (LRGUK), mRNA [NM_144648] -3.0 BX088949 BX088949 BX088949 Soares_testis_NHT Homo sapiens cDNA clone IMAGp998O223514 ; IMAGE:1391853, mRNA sequence [BX088949] -3.0 THC2630554 Q920P1_MOUSE (Q920P1) Odorant receptor (Olfactory receptor Olfr74) (Olfactory receptor MOR174-4), partial (18%) [THC2630554] -3.0 NM_004932 CDH6 Homo sapiens cadherin 6, type 2, K-cadherin (fetal kidney) (CDH6), mRNA [NM_004932] -3.0 AI761615 THC2549122 AI761615 wg66e09.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE:2370088 3', mRNA sequence [AI761615] -3.0 NM_001078 VCAM1 Homo sapiens vascular cell adhesion molecule 1 (VCAM1), transcript variant 1, mRNA [NM_001078] -3.0 NM_194250 ZNF804A Homo sapiens (ZNF804A), mRNA [NM_194250] -3.0 NM_015320 ARHGEF4 Homo sapiens Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 1, mRNA [NM_015320] -3.0 AF072164 C9orf33 Homo sapiens HSFE-1 mRNA, partial cds. [AF072164] -3.0 U50277 U50277 Human breast cancer suppressor element Ishmael Upper CP1 mRNA, partial cds. [U50277] -3.0

Downloaded from BE007076 THC2757796 BE007076 PM3-BN0137-290300-002-g09 BN0137 Homo sapiens cDNA, mRNA sequence [BE007076] -3.1 NM_001511 CXCL1 Homo sapiens chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1), mRNA [NM_001511] -3.1 NM_213633 PSG4 Homo sapiens pregnancy specific beta-1-glycoprotein 4 (PSG4), transcript variant 2, mRNA [NM_213633] -3.1 AF268613 POU5F1P4 Homo sapiens POU 5 domain protein (POU5FLC1) mRNA, complete cds. [AF268613] -3.1 XM_929617 LOC441996 Unknown -3.1 NM_199077 CNNM2 Homo sapiens cyclin M2 (CNNM2), transcript variant 3, mRNA [NM_199077] -3.1 http://hyper.ahajournals.org/ NM_001006939 LRRC18 Homo sapiens leucine rich repeat containing 18 (LRRC18), mRNA [NM_001006939] -3.1 NR_001539 TTTY22 Homo sapiens testis-specific transcript, Y-linked 22 (TTTY22), non-coding RNA [NR_001539] -3.2 BX094246 BX094246 BX094246 Soares_testis_NHT Homo sapiens cDNA clone IMAGp998N201862, mRNA sequence [BX094246] -3.2 LOC729141 PREDICTED: Homo sapiens misc_RNA (LOC729141), miscRNA [XR_015463] -3.2 NM_024021 MS4A4A Homo sapiens membrane-spanning 4-domains, subfamily A, member 4 (MS4A4A), transcript variant 1, mRNA [NM_024021] -3.2 NM_145664 SPANXB2 Homo sapiens SPANX family, member B2 (SPANXB2), mRNA [NM_145664] -3.2 NM_080866 SLC22A9 Homo sapiens solute carrier family 22 (organic anion transporter), member 9 (SLC22A9), mRNA [NM_080866] -3.2 AF116605 IKZF1 Homo sapiens PRO0758 mRNA, complete cds. [AF116605] -3.2 byguest onSeptember11, 2012 NM_001486 GCKR Homo sapiens glucokinase (hexokinase 4) regulator (GCKR), mRNA [NM_001486] -3.3 AL137479 AK124882 Homo sapiens cDNA FLJ42892 fis, clone BRHIP3008565. [AK124882] -3.3 NM_031415 GSDMC Homo sapiens gasdermin C (GSDMC), mRNA [NM_031415] -3.3 NM_004591 CCL20 Homo sapiens chemokine (C-C motif) ligand 20 (CCL20), transcript variant 1, mRNA [NM_004591] -3.3 NM_000720 CACNA1D Homo sapiens calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant 1, mRNA [NM_000720] -3.4 LOC390213 PREDICTED: Homo sapiens similar to hCG21066 (LOC390213), mRNA [XM_001721422] -3.4 NM_152338 JCLN Homo sapiens jacalin-like lectin domain containing (JCLN), mRNA [NM_152338] -3.4 NM_022842 CDCP1 Homo sapiens CUB domain containing protein 1 (CDCP1), transcript variant 1, mRNA [NM_022842] -3.5 NM_007289 MME Homo sapiens membrane metallo-endopeptidase (MME), transcript variant 2b, mRNA [NM_007289] -3.5 NM_000773 CYP2E1 Homo sapiens cytochrome P450, family 2, subfamily E, polypeptide 1 (CYP2E1), mRNA [NM_000773] -3.5 AY465172 POTEC Homo sapiens POTE ankyrin domain family, member C (POTEC), mRNA [NM_001137671] -3.6 NM_001879 MASP1 Homo sapiens mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor) (MASP1), transcript variant 1, mRNA [NM_00187 -3.7 NM_175895 C12orf61 Homo sapiens chromosome 12 open reading frame 61 (C12orf61), mRNA [NM_175895] -3.7 AK094167 FLJ36848 Homo sapiens cDNA FLJ36848 fis, clone ASTRO2013802. [AK094167] -3.8 NM_022115 PRDM15 Homo sapiens PR domain containing 15 (PRDM15), transcript variant 1, mRNA [NM_022115] -3.8 NM_016279 CDH9 Homo sapiens cadherin 9, type 2 (T1-cadherin) (CDH9), mRNA [NM_016279] -3.8 AL136901 C10orf79 Homo sapiens mRNA; cDNA DKFZp434P078 (from clone DKFZp434P078). [AL136901] -3.9 NM_024873 TNIP3 Homo sapiens TNFAIP3 interacting protein 3 (TNIP3), transcript variant 1, mRNA [NM_024873] -3.9 NM_004049 BCL2A1 Homo sapiens BCL2-related protein A1 (BCL2A1), transcript variant 1, mRNA [NM_004049] -4.0 NM_145343 APOL1 Homo sapiens apolipoprotein L, 1 (APOL1), transcript variant 2, mRNA [NM_145343] -4.1 NM_003301 TRHR Homo sapiens thyrotropin-releasing hormone receptor (TRHR), mRNA [NM_003301] -4.4 NM_001710 CFB Homo sapiens complement factor B (CFB), mRNA [NM_001710] -4.5 NM_006398 UBD Homo sapiens ubiquitin D (UBD), mRNA [NM_006398] -4.6 AK000832 AK000832 Homo sapiens cDNA FLJ20825 fis, clone ADSE00160. [AK000832] -5.6 Downloaded from http://hyper.ahajournals.org/ byguest onSeptember11, 2012 Table S3: Gene ID of genes associated with H3K27me3 A4GALT ADAMTS14 ALK APLN ATAD4 BARX1 BTG4 C14orf126 A4GNT ADAMTS15 ALKBH3 APLP2 ATCAY BARX2 BTN1A1 C14orf132 AACS ADAMTS16 ALKBH8 APOBEC2 ATF3 BBX BTNL2 C14orf145 AADAT ADAMTS17 ALMS1 APOBEC3A ATG4C BCAN BTNL9 C14orf151 AARSL ADAMTS19 ALOX12B APOD ATG4D BCHE BTRC C14orf162 AATF ADAMTS20 ALOX15 APPBP1 ATG5 BCKDHB BUB1 C14orf165 ABAT ADAMTS5 ALOX15B AQP1 ATG9B BCL11A BUB1B C14orf166 ABCA13 ADAMTS7 ALOX5 AQP4 ATOH1 BCL11B BUB3 C14orf166B ABCB1 ADAMTS8 ALOXE3 AQP6 ATOH7 BCL2L11 BUD13 C14orf172 ABCB4 ADAMTSL1 ALPI AQP9 ATP11A BCL2L14 BZRAP1 C14orf29 ABCB5 ADAMTSL2 ALPK1 ARC ATP12A BCMO1 C10orf113 C14orf37 ABCB7 ADAMTSL3 ALPK2 ARFGAP1 ATP13A4 BCOR C10orf114 C14orf39 ABCC13 ADAR ALPL ARFGAP3 ATP1A1 BCORL1 C10orf121 C14orf50 ABCC5 ADC ALPP ARFIP1 ATP1A2 BDH1 C10orf128 C14orf70 ABCC8 ADCK2 ALPPL2 ARG2 ATP1A3 BDNF C10orf132 C14orf8 ABCC9 ADCY1 ALS2CR7 ARGFX ATP1A4 BDP1 C10orf22 C14orf80 ABCG1 ADCY2 ALX3 ARHGAP24 ATP1B2 BEAN C10orf27 C15orf27 ABCG4 ADCY5 ALX4 ARHGAP25 ATP1B4 BECN1 C10orf28 C15orf48 ABCG5 ADCY8 AMAC1 ARHGAP26 ATP2A3 BEX2 C10orf35 C16orf35 ABCG8 ADCYAP1 AMICA1 ARHGAP4 ATP2B2 BHLHB3 C10orf39 C16orf44 ABHD1 ADCYAP1R1 AMIGO1 ARHGAP8 ATP2B3 BHLHB4 C10orf4 C16orf54 ABHD6 ADD2 AMMECR1 ARHGAP9 ATP4A BHLHB5 C10orf41 C16orf63 ABHD9 ADM2 AMN ARHGEF11 ATP4B BHMT C10orf46 C16orf67 ABLIM2 ADORA1 AMOT ARHGEF18 ATP5J2 BHMT2 C10orf53 C16orf68 ABO ADORA2A AMPD1 ARHGEF19 ATP5O BIK C10orf6 C16orf69 ABP1 ADPGK ANGEL1 ARHGEF4 ATP6AP2 BIN1 C10orf67 C17orf37 ABR ADPN ANGPT4 ARHGEF7 ATP6V1B1 BIRC7 C10orf72 C17orf50 ABT1 ADRA1A ANK1 ARHGEF9 ATP6V1B2 BLK C10orf79 C17orf73 ABTB2 ADRA1B ANK2 ARID3C ATP6V1C2 BLMH C10orf80 C17orf76 ACAD9 ADRA1D ANK3 ARID5A ATP6V1G1 BLNK C10orf81 C18orf1 ACADL ADRA2A ANKFN1 ARL5B ATP6V1H BLOC1S1 C10orf9 C18orf16 ACBD6 ADRA2B ANKRD15 ARL5C ATP8A1 BLR1 C10orf93 C18orf20 ACCN1 ADRA2C ANKRD18A ARMC4 ATP8A2 BMF C10orf95 C18orf21 ACCN2 ADRB3 ANKRD19 ARMC8 ATR BMP3 C10orf96 C18orf22 ACCN4 ADRBK2 ANKRD20A3 ARMCX2 ATRN BMP7 C10orf97 C18orf23 ACE AEBP1 ANKRD20B ARMCX5 ATRNL1 BMPR1A C11orf11 C18orf24 ACE2 AFF2 ANKRD28 ARMCX6 ATXN10 BMS1L C11orf36 C18orf34 ACHE AFF3 ANKRD29 ARNT2 ATXN7L2 BMX C11orf39 C18orf45 ACMSD AFTIPHILIN ANKRD38 ARNTL AUH BNC1 C11orf43 C18orf51 ACOXL AGC1 ANKRD40 ARPC2 AUTS2 BNIP3L C11orf45 C19orf16 ACPL2 AGGF1 ANKRD43 ARPP-21 AVP BOC C11orf46 C19orf2 ACPP AGPAT6 ANKRD44 ARR3 AVPR1A BOLA1 C11orf51 C19orf30 ACSBG1 AGTPBP1 ANKRD45 ARSI AVPR1B BPESC1 C11orf63 C19orf35 ACSL6 AGTR1 ANKRD46 ART5 AVPR2 BPIL2 C11orf64 C19orf4 ACSS1 AGTR2 ANKRD5 ARTN AYTL2 BRAF C11orf72 C1orf106 ACTA1 AGTRL1 ANKRD6 ARX AZGP1 BRD1 C11orf75 C1orf116 ACTC AGXT ANKRD7 AS3MT B3GALT5 BRD2 C11orf9 C1orf120 ACTG2 AHDC1 ANKS6 ASAH2 B3GAT1 BRD3 C12orf28 C1orf126 ACTL6B AHSG ANTXR1 ASAM B3GAT2 BRD7 C12orf42 C1orf127 ACTN3 AIFL ANXA13 ASB10 B3GNT2 BRMS1L C12orf5 C1orf130 ACTR10 AIG1 ANXA2 ASB13 B3GNT3 BRPF1 C12orf53 C1orf140 ACTR3B AIM1 ANXA5 ASB4 B3GNT4 BRS3 C12orf54 C1orf147 ACTR5 AIM2 ANXA7 ASB6 B3Gn-T6 BRSK1 C12orf59 C1orf159 ACVR1C AIPL1 ANXA8 ASB8 B4GALNT1 BRSK2 C13orf10 C1orf160 ACY1L2 AJAP1 AP1B1 ASCC3 B4GALNT2 BRUNOL4 C13orf18 C1orf161 ADA AK5 AP1M2 ASCL1 B4GALNT3 BRUNOL5 C13orf25 C1orf164 ADAM12 AKAP1 AP1S1 ASCL2 B4GALNT4 BSN C13orf26 C1orf165 ADAM21 AKAP6 AP1S2 ASCL4 BAHD1 BSND C13orf7 C1orf166 ADAM22 AKR1B10 AP2B1 ASMTL BAI1 BSPRY C14orf101 C1orf170 ADAM30 ALDH1A2 AP3B2 ASPHD2 BAI2 BST1 C14orf102 C1orf172 ADAM33 ALDH1A3 APBB1 ASRGL1 BAI3 BTBD10 C14orf105 C1orf177 ADAM5 ALDH1L1 APBB2 ASS BAIAP3 BTBD11 C14orf112 C1orf187 ADAM7 ALDH1L2 APC2 ASTL BAMBI BTBD4 C14orf115 C1orf19 ADAM8 ALDH3B2 APCDD1 ASTN BAPX1 BTC C14orf118 C1orf210 ADAMTS12 ALG14 APEG1 ASXL2 BARHL1 BTF3 C14orf120 C1orf211 ADAMTS13 ALG6 APEX2 ASZ1 BARHL2 BTG2 C14orf121 C1orf24 Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 C1orf32 C20orf79 C8orf13 CACNG5 CCDC94 CDH17 CHMP2B CMYA3 C1orf34 C20orf85 C8orf14 CACNG7 CCDC99 CDH2 CHN2 CMYA5 C1orf36 C20orf86 C8orf33 CACNG8 CCK CDH22 CHODL CNDP1 C1orf42 C21orf121 C8orf34 CADPS CCKAR CDH23 CHPF CNDP2 C1orf49 C21orf129 C8orf47 CADPS2 CCKBR CDH3 CHRD CNFN C1orf53 C21orf24 C8orf53 CALB1 CCL11 CDH4 CHRDL1 CNGA3 C1orf61 C21orf37 C8orf55 CALB2 CCL13 CDH6 CHRFAM7A CNGA4 C1orf62 C21orf51 C8orf68 CALCA CCL18 CDH7 CHRM1 CNGB1 C1orf65 C21orf55 C8orf77 CALCB CCL19 CDH9 CHRM2 CNIH C1orf75 C21orf62 C8orf79 CALCOCO1 CCL21 CDK4 CHRM4 CNIH2 C1orf76 C21orf69 C8ORFK23 CALCR CCL24 CDK5R2 CHRNA2 CNIH3 C1orf87 C21orf82 C9orf105 CALML5 CCL26 CDK5RAP2 CHRNA3 CNKSR2 C1orf88 C21orf88 C9orf11 CALN1 CCL3 CDK8 CHRNA7 CNN1 C1orf92 C21orf89 C9orf121 CAMK1D CCL3L1 CDKN1C CHRNA9 CNNM1 C1orf94 C21orf96 C9orf122 CAMK1G CCL3L3 CDKN2A CHRNB1 CNNM2 C1orf95 C2orf14 C9orf125 CAMK2A CCL7 CDKN3 CHRNB2 CNR1 C1orf96 C2orf19 C9orf127 CAMK2B CCL8 CDO1 CHRNB4 CNTD2 C1QA C2orf26 C9orf152 CAMK2N1 CCNA1 CDS1 CHRND CNTFR C1QB C3orf14 C9orf156 CAMK2N2 CCNC CDX1 CHST13 CNTN1 C1QDC2 C3orf18 C9orf18 CAMKV CCND2 CDX2 CHST4 CNTN2 C1QL1 C3orf19 C9orf21 CAMP CCNE1 CDX4 CHST5 CNTN4 C1QL2 C3orf22 C9orf24 CAMTA1 CCNG1 CDYL CHST6 CNTN5 C1QL3 C3orf23 C9orf25 CAPN5 CCNG2 CEACAM20 CHST8 CNTN6 C1QL4 C3orf26 C9orf29 CAPN6 CCR2 CEACAM21 CHURC1 CNTROB C1QTNF1 C3orf27 C9orf33 CARD14 CCR5 CEACAM5 CHX10 COBL C1QTNF2 C3orf30 C9orf36 CARD15 CCR7 CEACAM6 CIB2 COCH C1QTNF4 C3orf31 C9orf39 CART CCR9 CEBPA CIB4 COG2 C1QTNF8 C3orf32 C9orf4 CASKIN1 CCT7 CEBPG CIITA COG3 C1R C3orf36 C9orf42 CASR CD164L2 CECR5 CILP COL11A1 C1S C3orf39 C9orf50 CAST1 CD1D CECR6 CILP2 COL11A2 C2 C3orf46 C9orf53 CASZ1 CD207 CEECAM1 CIT COL12A1 C20orf102 C3orf56 C9orf57 CAT CD22 CEI CITED1 COL13A1 C20orf103 C3orf57 C9orf58 CAV3 CD248 CELSR1 CKB COL14A1 C20orf112 C4orf19 C9orf61 CBLN1 CD300A CELSR2 CLCNKA COL15A1 C20orf114 C5orf20 C9orf62 CBLN2 CD300LF CENTA1 CLCNKB COL16A1 C20orf121 C6orf108 C9orf65 CBWD2 CD33L3 CENTA2 CLDN1 COL19A1 C20orf127 C6orf114 C9orf71 CBWD3 CD34 CENTD1 CLDN10 COL1A1 C20orf135 C6orf118 C9orf72 CBX2 CD38 CENTG1 CLDN12 COL21A1 C20orf141 C6orf125 CA1 CBX4 CD3D CENTG2 CLDN18 COL23A1 C20orf149 C6orf129 CA11 CBX6 CD3E CEP152 CLDN2 COL24A1 C20orf151 C6orf141 CA14 CBX8 CD3G CERKL CLDN23 COL25A1 C20orf152 C6orf15 CA2 CCBE1 CD4 CETN1 CLDN9 COL27A1 C20orf160 C6orf155 CA3 CCDC102A CD40 CFC1 CLDND1 COL2A1 C20orf166 C6orf159 CA4 CCDC102B CD6 CFD CLEC11A COL4A1 C20orf174 C6orf167 CA5A CCDC14 CD72 CFTR CLEC4E COL4A3 C20orf175 C6orf168 CA5B CCDC15 CD83 CGA CLEC4F COL4A4 C20orf179 C6orf174 CA5BL CCDC27 CD86 CGN CLEC4G COL4A5 C20orf19 C6orf190 CA7 CCDC3 CD8A CGREF1 CLIC5 COL4A6 C20orf26 C6orf194 CAB39 CCDC33 CDA CGRRF1 CLIC6 COL5A3 C20orf28 C6orf195 CABP1 CCDC36 CDC14B CH25H CLK3 COL6A1 C20orf3 C6orf205 CABP7 CCDC37 CDC16 CHAD CLK4 COL6A2 C20orf39 C6orf208 CACNA1A CCDC38 CDC2 CHAT CLMN COL6A3 C20orf4 C6orf213 CACNA1C CCDC4 CDC25A CHD5 CLN5 COL7A1 C20orf42 C6orf218 CACNA1D CCDC48 CDC2L6 CHD6 CLPB COL9A1 C20orf45 C6orf32 CACNA1E CCDC52 CDC37 CHDH CLPS COL9A2 C20orf46 C6orf54 CACNA1G CCDC54 CDC42BPG CHGA CLSTN2 COLEC12 C20orf51 C6orf55 CACNA1H CCDC63 CDC5L CHGB CLUL1 COLQ C20orf54 C6orf60 CACNA1S CCDC64 CDC7 ChGn CLYBL COMMD9 C20orf58 C6orf66 CACNA2D3 CCDC67 CDCA7 CHI3L1 CMAS COMP C20orf59 C7orf16 CACNA2D4 CCDC7 CDCP1 CHI3L2 CMIP COPB2 C20orf62 C7orf33 CACNB2 CCDC73 CDH1 CHIA CMKLR1 COPS4 C20orf71 C7orf9 CACNB4 CCDC8 CDH13 CHIT1 CMKOR1 COPS7A C20orf75 C8orf12 CACNG4 CCDC93 CDH16 CHM CML2 CORO2B

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 COVA1 CRYGD CXXC5 DEFB1 DKFZP686A0 DOC2A EBF3 EML5 COX4I2 CRYGN CXXC6 DEFB104A 1247 DOCK11 ECAT11 EMP2 COX6A1 CRYM CYBA DEFB112 DKFZp686K1 DOCK3 ECE2 EMX1 COX6A2 CSDC2 CYFIP2 DEFB124 684 DOCK5 ECEL1 EMX2 COX6B2 CSF1 CYGB DEFB128 DKFZp761B1 DOCK8 ECEL1P2 EN1 COX7A2 CSF1R CYP11A1 DEFB129 07 DOCK9 ECHDC2 EN2 COX7B2 CSF2RB CYP19A1 DEFB134 DKFZp761E1 DOK2 ECRG4 ENPEP CPA3 CSF3R CYP1A1 DEFB136 98 DOK5 EDA ENPP1 CPA6 CSH1 CYP1A2 DEFB137 DKFZp779B1 DOK6 EDAR ENPP3 CPAMD8 CSHL1 CYP1B1 DEGS2 540 DP58 EDARADD ENPP4 CPB1 CSMD1 CYP24A1 DEK DKFZP781I1 DPCR1 EDG4 ENPP5 CPD CSMD3 CYP26A1 DENND1A 119 DPEP1 EDG7 ENTPD2 CPEB1 CSNK1G3 CYP26B1 DENND1C DLD DPEP3 EDG8 ENTPD3 CPLX1 CSNK2A1 CYP27B1 DENND2A DLEC1 DPF1 EDN3 ENTPD4 CPLX2 CSNK2A1P CYP2A13 DENND2C DLEU7 DPF3 EDNRA ENTPD6 CPLX3 CSPG2 CYP2A6 DENND2D DLEU8 DPH1 EED EOMES CPLX4 CSPG3 CYP2A7 DEPDC6 DLG3 DPP10 EEF1A1 EPB41L1 CPM CSPG5 CYP2B6 DEPDC7 DLG5 DPY19L2 EEF1A2 EPB41L4B CPN1 CSPG6 CYP2E1 DES DLGAP4 DPYSL4 EEFSEC EPB42 CPNE4 CSRP2 CYP2F1 DFNA5 DLK1 DPYSL5 EFCAB1 EPB49 CPNE6 CSRP2BP CYP2J2 DFNB31 DLL1 DQX1 EFCBP1 EPC2 CPNE9 CST9 CYP2S1 DGAT2 DLL3 DRB1 EFCBP2 EPDR1 CPOX CSTB CYP3A4 DGAT2L3 DLL4 DRD1 EFHA1 EPHA10 CPSF6 CSTF2 CYP3A43 DGAT2L4 DLX1 DRD1IP EFHC2 EPHA4 CPT1B CSTL1 CYP46A1 DGAT2L6 DLX2 DRD2 EFHD1 EPHA5 CPVL CTDSP2 CYP4B1 DGAT2L7 DLX3 DRD3 EFNA2 EPHA6 CPXM CTGLF1 CYP4F11 DGCR14 DLX4 DRD4 EFNA3 EPHA7 CPXM2 CTLA4 CYP7B1 DGKG DLX5 DREV1 EFNB1 EPHA8 CPZ CTNNA3 CYP8B1 DGKI DLX6 DRP2 EFNB3 EPHB1 CR1 CTNNB1 CYS1 DGKK DMBT1 DSC2 EFS EPHB3 CR1L CTNNBL1 CYTL1 DGKZ DMBX1 DSC3 EGFL6 EPHB6 CR2 CTNND2 D21S2056E DGUOK DMC1 DSCAML1 EGFR EPHX2 CRABP1 CTPS2 D4S234E DHRS2 DMN DSCR1 EGLN3 EPN3 CRABP2 CTSD DAAM1 DHTKD1 DMRT1 DSCR10 EGR2 EPO CRADD CTSE DAAM2 DHX15 DMRT2 DSCR1L1 EGR3 EPS8L1 CRB2 CTSL2 DAB1 DIAPH2 DMRT3 DSCR1L2 EGR4 ERAF CRB3 CTTNBP2 DACH2 DIO1 DMRTA2 DSCR4 EHD3 ERAS CRBN CUEDC1 DACT1 DIO3 DMRTB1 DSCR6 EHF ERBB3 CREB3L1 CUEDC2 DACT2 DIP DMRTC1 DSCR8 EIF1 ERBB4 CREBL1 CUGBP2 DAD1 DIRAS1 DMRTC2 DSG2 EIF2AK3 ERG CREG1 CUTL2 DAPK2 DIRAS2 DMXL2 DSG3 EIF3S3 ERICH1 CREG2 CX36 DARS DISP2 DNAH11 DSP EIF3S6 ERN2 CRH CX40.1 DAZL DKFZp434B1 DNAH17 DTNA EIF4E3 ERO1LB CRHBP CX62 DBC1 231 DNAH5 DTNB EIF5 ESPN CRHR1 CXCL10 DBR1 DKFZp434E1 DNAH7 DTWD2 ELA2 ESPNP CRHR2 CXCL12 DBX1 119 DNAI1 DTX1 ELAVL2 ESR1 CRI2 CXCL13 DBX2 DKFZP434L1 DNAJA4 DTX2 ELAVL3 ESRRB CRIP3 CXCL14 DCD 87 DNAJB2 DTX3 ELAVL4 ESRRG CRISPLD2 CXCL9 DCDC2 DKFZp434N0 DNAJB8 DUOX1 ELF5 ESX1 CRLF1 CXCR4 DCHS2 62 DNAJC11 DUOX2 ELMO2 ETFA CRMP1 CXorf12 DCP1A DKFZp434O0 DNAJC12 DUSP19 ELMOD1 ETNK1 CROP CXorf23 DCP1B 527 DNAJC19 DUSP22 ELMOD2 ETS1 CRSP2 CXorf25 DCTD DKFZp547E0 DNAJC5B DUSP26 ELOVL2 ETV5 CRSP3 CXorf36 DCTN1 87 DNAJC5G DUSP4 ELOVL3 ETV7 CRSP8 CXorf38 DCTN2 DKFZP564J1 DNAL4 DUSP9 ELOVL4 EVA1 CRTAC1 CXorf39 DCUN1D5 02 DNASE2 DYNC2H1 ELOVL7 EVL CRX CXorf41 DCX DKFZP564O DNER DYNLL2 ELP3 CRY1 CXorf44 DDC 0823 DNHD3 DYNLRB2 EMB EXDL2 CRYAB CXorf48 DDR1 DKFZP566N0 DNM1 DYRK3 EMID1 EXOC1 CRYBA2 CXorf50 DDX11 34 DNM1DN8-2 DYRK4 EMID2 EXPH5 CRYBB1 CXorf55 DDX25 DKFZP586H2 DNMT3A EMILIN2 EXT2 CRYBB2 CXXC1 DDX48 123 DNPEP E2F8 EMILIN3 EYA1 CRYGC CXXC4 DDX6 DNTT EBF2 EML2 EYA2

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 EYA4 FBXL21 FLJ10324 FLJ32447 FLJ44477 FOXO3A GALNT17 GJA3 F10 FBXL8 FLJ10357 FLJ32658 FLJ44635 FOXP2 GALNT8 GJA4 F12 FBXO21 FLJ10781 FLJ32682 FLJ44790 FOXP4 GALNT9 GJA5 F13A1 FBXO25 FLJ10916 FLJ32784 FLJ44815 FOXQ1 GALNTL1 GJA8 F7 FBXO3 FLJ11171 FLJ32786 FLJ44817 FOXR1 GALNTL5 GJB1 F8A1 FBXO39 FLJ12334 FLJ32871 FLJ44874 FRAS1 GALR1 GJB2 FA2H FBXO40 FLJ12505 FLJ32926 FLJ44881 FRAT2 GALR2 GJB3 FAAH FBXO7 FLJ12684 FLJ32997 FLJ44968 FREM2 GAN GJB4 FABP3 FBXW12 FLJ12700 FLJ33387 FLJ45079 FRK GAP43 GJB6 FABP5 FBXW8 FLJ13236 FLJ33534 FLJ45187 FRMD3 GAPDHS GJE1 FADD FCAMR FLJ13310 FLJ33718 FLJ45231 FRMD4A GARNL1 GK2 FADS6 FCGBP FLJ13391 FLJ33790 FLJ45300 FRMPD1 GART GKN1 FAIM2 FCGR3B FLJ14001 FLJ34690 FLJ45455 FRMPD4 GAS1 GLCE FAM101A FCHO2 FLJ14213 FLJ35695 FLJ45557 FRZB GAS2 GLDC FAM105A FCMD FLJ14712 FLJ35767 FLJ45803 FSIP2 GAS2L2 GLDN FAM111B FCN1 FLJ14816 FLJ35773 FLJ45825 FSTL4 GAS2L3 GLI1 FAM112B FCRL6 FLJ16008 FLJ35784 FLJ45950 FTCD GAS6 GLI2 FAM11A FCRLM1 FLJ16124 FLJ35816 FLJ46020 FTO GAST GLIS1 FAM12A FCRLM2 FLJ16165 FLJ35848 FLJ46082 FTSJ1 GATA4 GLIS3 FAM12B FDXR FLJ16171 FLJ35880 FLJ46154 FUBP3 GATA5 GLP1R FAM13C1 FER1L4 FLJ16478 FLJ36032 FLJ46156 FUCA2 GATA6 GLRA3 FAM14A FERD3L FLJ20105 FLJ36749 FLJ46266 FUNDC1 GATAD1 GLS2 FAM19A2 FEV FLJ20152 FLJ36951 FLJ46347 FUNDC2 GATM GLT25D2 FAM19A4 FGD2 FLJ20366 FLJ37034 FLJ46536 FUSSEL18 GATS GLT28D1 FAM19A5 FGD3 FLJ20534 FLJ37078 FLJ46831 FUT4 GBL GLT8D2 FAM20B FGF1 FLJ20628 FLJ37357 FLJ46836 FXYD1 GC GLYATL1 FAM26A FGF10 FLJ21062 FLJ37478 FLJ90166 FXYD2 GCET2 GLYCAM1 FAM3B FGF11 FLJ21438 FLJ37543 FLJ90231 FXYD4 GCK GMCL1L FAM43B FGF13 FLJ21511 FLJ38377 FLJ90650 FXYD6 GCLC GMFB FAM44A FGF14 FLJ21865 FLJ38596 FLJ90680 FXYD7 GCM2 GMPPA FAM44C FGF17 FLJ21901 FLJ39155 FLT3 FZD10 GCNT3 GMPS FAM46C FGF19 FLJ22184 FLJ39237 FLT4 FZD2 GDA GNAL FAM51A1 FGF23 FLJ22374 FLJ39501 FLVCR FZD3 GDAP1 GNAO1 FAM57B FGF3 FLJ22596 FLJ39639 FMN1 FZD7 GDAP1L1 GNAS FAM59A FGF4 FLJ22662 FLJ39739 FMN2 G0S2 GDDR GNG13 FAM5B FGF5 FLJ22746 FLJ39743 FMNL1 G6PC2 GDF10 GNG2 FAM5C FGF6 FLJ23191 FLJ40288 FMNL3 GABARAPL2 GDF7 GNG7 FAM62C FGF8 FLJ23577 FLJ40365 FMOD GABBR1 GDNF GNG8 FAM64A FGF9 FLJ23657 FLJ40411 FNDC1 GABBR2 GDPD2 GNL2 FAM70A FGFBP1 FLJ23834 FLJ40473 FNDC3A GABRA2 GDPD4 GOLGA2LY1 FAM77C FGFR2 FLJ25037 FLJ40504 FNDC5 GABRA4 GDPD5 GOLGA5 FAM77D FGFR3 FLJ25067 FLJ40869 FNDC7 GABRA5 GEFT GOLGA7 FAM78A FGFRL1 FLJ25084 FLJ41170 FNTB GABRB1 GEM GOPC FAM78B FGG FLJ25169 FLJ41423 FOLR2 GABRB2 GFER GOSR2 FAM80A FGL1 FLJ25369 FLJ41603 FOLR3 GABRB3 GFI1 GOT1 FAM81A FGR FLJ25422 FLJ41649 FOSL2 GABRD GFI1B GP1BB FAM81B FH FLJ25444 FLJ41841 FOXA1 GABRQ GFPT2 GP5 FAM82A FHIT FLJ25477 FLJ41993 FOXA2 GABRR1 GFRA1 GP9 FAM83B FHL1 FLJ25530 FLJ42133 FOXA3 GAD1 GFRA3 GPAM FAM83F FHL2 FLJ25770 FLJ42220 FOXB1 GAD2 GFRA4 GPATC2 FAM83H FHOD3 FLJ26443 FLJ42461 FOXC1 GADD45G GGA1 GPC1 FAM84A FIBCD1 FLJ30294 FLJ42486 FOXC2 GADL1 GGT6 GPC3 FAM89A FIGF FLJ30313 FLJ42875 FOXD1 GAL GGTL3 GPC4 FAM91A1 FIGLA FLJ30375 FLJ42953 FOXD2 GAL3ST1 GGTLA4 GPC5 FAM92A3 FIGN FLJ30707 FLJ43390 FOXD3 GAL3ST3 GH1 GPHB5 FAM99A FIGNL1 FLJ30834 FLJ43706 FOXD4L4 GALNAC4S- GHITM GPIAP1 FANCF FIP1L1 FLJ30901 FLJ43826 FOXE1 6ST GHR GPM6B FAS FJX1 FLJ30934 FLJ43870 FOXE3 GALNACT-2 GHRH GPNMB FAT FKBP4 FLJ31875 FLJ44005 FOXF1 GALNT1 GHRHR GPR1 FBLN1 FKBP6 FLJ31951 FLJ44006 FOXF2 GALNT11 GHSR GPR101 FBLN5 FKBPL FLJ32011 FLJ44076 FOXG1B GALNT12 GIP GPR103 FBN3 FLI1 FLJ32063 FLJ44186 FOXJ1 GALNT13 GIPR GPR109B FBXL16 FLJ10241 FLJ32110 FLJ44313 FOXL2 GALNT14 GJA1 GPR112

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 GPR114 GRIN2C HDAC11 HOXA13 HTR3D IL12B IREB2 KCNA2 GPR119 GRIN2D HDAC6 HOXB1 HTR4 IL12RB2 IRF1 KCNA3 GPR12 GRIN3B HDAC8 HOXB13 HTR5A IL13 IRF2BP2 KCNA4 GPR120 GRK1 HDAC9 HOXB8 HTR6 IL17 IRF4 KCNA5 GPR137 GRM1 HDC HOXB9 HTR7 IL17C IRF5 KCNA6 GPR137B GRM2 HEAB HOXC10 HTRA1 IL17E IRF8 KCNA7 GPR139 GRM3 HECW2 HOXC11 HUNK IL17F IRS1 KCNAB1 GPR142 GRM4 HELB HOXC12 HUWE1 IL17RB IRS2 KCNB1 GPR144 GRM7 HELT HOXC13 ICAM4 IL17RD IRS4 KCNB2 GPR148 GRM8 HEMGN HOXC4 ICAM5 IL18RAP IRX1 KCNC1 GPR149 GRP HERC2 HOXC5 ID3 IL19 IRX2 KCNC2 GPR15 GRPEL1 HERC5 HOXC6 ID4 IL1B IRX3 KCNC3 GPR156 GRPR HES3 HOXC8 IDH3B IL1F10 IRX4 KCND2 GPR158 GRSF1 HES5 HOXC9 IER3IP1 IL1F5 IRX5 KCNE1 GPR160 GRTP1 HES6 HOXD10 IER5L IL1F7 IRX6 KCNE2 GPR173 GSC HEY2 HOXD11 IFITM5 IL1F8 ISG20L1 KCNE3 GPR178 GSCL HEYL HOXD12 IFNA1 IL1R2 ISL1 KCNF1 GPR19 GSDM1 HFE2 HOXD13 IFNA13 IL1RAPL2 ISL2 KCNG1 GPR20 GSH1 HFM1 HOXD9 IFNA2 IL1RN ISLR2 KCNG2 GPR25 GSH2 HGF HPCA IFNA5 IL20RA ISOC1 KCNG3 GPR27 GSPT2 HHCM HPCAL4 IFNA6 IL21 ISOC2 KCNG4 GPR37L1 GSTK1 HIC2 HPGD IFNA8 IL22RA1 ITGA11 KCNH1 GPR45 GSTM5 HIF3A HPSE2 IFNAR1 IL27 ITGA4 KCNH2 GPR50 GSTP1 HIG2 HPX IFNAR2 IL28A ITGA7 KCNH3 GPR55 GUCA1A HIPK1 HR IFNB1 IL28B ITGA8 KCNH4 GPR6 GUCA2B HIST1H2AI HRASLS5 IFNK IL28RA ITGA9 KCNH5 GPR61 GUCY1A2 HIST1H4H HRC IGBP1 IL29 ITGAL KCNH6 GPR62 GUCY1B2 HIST3H2A HRH2 IGF1R IL2RB ITGAM KCNH7 GPR64 GUCY2C HIST3H3 HRH3 IGF2 IL2RG ITGB2 KCNH8 GPR78 GUCY2D HK1 HRK IGF2AS IL3 ITIH1 KCNIP1 GPR81 GUCY2F HK2 HS1BP3 IGF2BP1 IL31 ITIH2 KCNIP3 GPR82 GUF1 HLA-B HS3ST1 IGF2BP3 IL5 ITIH5 KCNIP4 GPR83 GVIN1 HLA-C HS3ST2 IGF2R IL7 ITIH5L KCNJ1 GPR84 GYG2 HLA-DMA HS3ST3A1 IGFBP1 IL8RA ITLN2 KCNJ10 GPR85 GYLTL1B HLA-DMB HS3ST3B1 IGFBP3 IL9 ITM2C KCNJ11 GPR97 H19 HLA-DOA HS3ST4 IGFBP5 ILDR1 ITPR1 KCNJ15 GPRASP1 H2AFY HLA-DOB HS3ST5 IGFBP6 ILKAP JAKMIP1 KCNJ3 GPRASP2 H2AFY2 HLA-DPA1 HS3ST6 IGFBPL1 IMAA JAKMIP2 KCNJ4 GPRC5B HADH2 HLA-DPB1 HS6ST2 IGFL1 IMP5 JAM2 KCNJ5 GPRC5C HAMP HLA-DPB2 HS6ST3 IGFL2 IMPACT JARID1B KCNJ6 GPRC6A HAND1 HLA-DQA2 HSD11B1 IGFL3 IMPDH1 JMJD1A KCNJ8 GPT2 HAND2 HLA-DQB2 HSD11B2 IGFL4 INA JMJD2A KCNJ9 GPX3 HAP1 HLA-DRA HSD17B12 IGLC1 INCENP JMJD5 KCNK1 GPX7 HAPLN2 HLA-G HSD17B3 IGLC2 INHA JPH1 KCNK12 GRAP2 HAPLN4 HLF HSF2 IGLV2-14 INHBB JPH2 KCNK13 GRB10 HAS1 HLXB9 HSF4 IGLV6-57 INMT JPH3 KCNK15 GRB7 HBA1 HMFN0672 HSPA12A IGSF1 INPP4B JPH4 KCNK16 GREM1 HBA2 HMFN0839 HSPA2 IGSF10 INSIG2 JRKL KCNK17 GRHL1 HBM HMG2L1 HSPB3 IGSF11 INSL4 JUP KCNK18 GRHL2 HBQ1 HMG4L HSPC049 IGSF21 INSL5 K6HF KCNK2 GRHL3 HBS1L HMGA1 HSPC065 IGSF22 INSL6 K6IRS2 KCNK3 GRIA1 HBZ HMGCLL1 HSPC105 IGSF3 INSM1 K6IRS3 KCNK4 GRIA2 HCA112 HMGCS2 HSPC159 IGSF4B INSM2 K6IRS4 KCNK5 GRIA4 HCFC2 HMGN1 HTATIP2 IGSF4C INSRR KA21 KCNK9 GRID1 HCG4P6 HMX2 HTATSF1 IGSF4D INTS1 KAAG1 KCNMA1 GRID2 HCG9 HMX3 HTLF IGSF8 INTS10 KAL1 KCNMB1 GRIK2 HCK HNF4A HTR1A IGSF9 IPF1 KALRN KCNN1 GRIK3 HCN1 HNRNPG-T HTR1E IGSF9B IPO11 KBTBD1 KCNN2 GRIK4 HCN4 HNRPAB HTR2A IHH IPO8 KBTBD11 KCNN3 GRIK5 HCP1 HNT HTR2C IHPK3 IQGAP2 KBTBD5 KCNQ1 GRIN1 HCRP1 HOMER2 HTR3A IL10 IQGAP3 KBTBD8 KCNQ1DN GRIN2A HCRTR2 HOOK1 HTR3B IL10RA IQSEC2 KCNA1 KCNQ3 GRIN2B HCTSL-s HORMAD2 HTR3C IL11 IRAK1 KCNA10 KCNQ4

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 KCNQ5 KIAA1751 KRT24 LCA10 LMX1B LOC284912 LOC390414 LOC554208 KCNS1 KIAA1754L KRT25D LCE5A LOC113444 LOC285016 LOC390594 LOC554223 KCNS2 KIAA1772 KRT2A LCK LOC114984 LOC285382 LOC390616 LOC554250 KCNS3 KIAA1794 KRT2B LCMT2 LOC115098 LOC285484 LOC390811 LOC57399 KCNV1 KIAA1804 KRT3 LCN10 LOC120376 LOC285498 LOC390874 LOC613212 KCTD1 KIAA1822 KRT4 LCN2 LOC126520 LOC285577 LOC390937 LOC619207 KCTD12 KIAA1822L KRT5 LCN8 LOC126860 LOC285735 LOC391799 LOC63928 KCTD15 KIAA1862 KRT6A LDB3 LOC128439 LOC285888 LOC391859 LOC641515 KCTD18 KIAA1900 KRT6B LDLRAP1 LOC129285 LOC285989 LOC392275 LOC641522 KCTD19 KIAA1904 KRT6C LDOC1L LOC130576 LOC286177 LOC392510 LOC641702 KCTD8 KIAA1909 KRT6IRS LECT1 LOC130951 LOC338328 LOC392843 LOC642268 KDELR1 KIAA1913 KRT6L LECT2 LOC133619 LOC339524 LOC399706 LOC642287 KDELR3 KIAA1914 KRT9 LEF1 LOC133993 LOC339778 LOC399818 LOC642299 KEL KIAA1919 KRTAP10-1 LEFTY2 LOC136288 LOC340228 LOC399829 LOC642325 KHDRBS3 KIAA1984 KRTAP10-12 LELP1 LOC136306 LOC340260 LOC399947 LOC642366 KIAA0087 KIF12 KRTAP10-2 LEMD1 LOC138046 LOC340508 LOC400120 LOC642405 KIAA0103 KIF17 KRTAP10-3 LEMD2 LOC138255 LOC340598 LOC400197 LOC642420 KIAA0157 KIF1A KRTAP10-6 LEMD3 LOC144233 LOC342865 LOC400553 LOC642432 KIAA0247 KIF25 KRTAP10-7 LEP LOC144481 LOC342897 LOC400707 LOC642452 KIAA0256 KIF26A KRTAP12-1 LEPROT LOC145757 LOC344191 LOC400891 LOC642491 KIAA0284 KIF2C KRTAP2-1 LGALS12 LOC146177 LOC345079 LOC400950 LOC642503 KIAA0427 KIF3B KRTAP2-2 LGALS13 LOC146325 LOC345643 LOC401056 LOC642534 KIAA0494 KIF3C KRTAP2-4 LGALS2 LOC146439 LOC346673 LOC401068 LOC642563 KIAA0495 KIF5A KRTAP3-2 LGI1 LOC150051 LOC348840 LOC401070 LOC642564 KIAA0514 KIF5C KRTAP3-3 LGI2 LOC152586 LOC349136 LOC401087 LOC642587 KIAA0523 KIFC2 KRTAP5-2 LGI3 LOC153328 LOC352909 LOC401089 LOC642597 KIAA0528 KIRREL KRTAP5-3 LGI4 LOC154822 LOC374491 LOC401222 LOC642644 KIAA0562 KIRREL2 KRTAP5-4 LGICZ1 LOC157740 LOC375133 LOC401268 LOC642645 KIAA0644 KIRREL3 KRTAP5-6 LGR5 LOC158301 LOC375323 LOC401399 LOC642654 KIAA0672 KIT KRTHA2 LGR6 LOC161527 LOC375449 LOC401433 LOC642691 KIAA0690 KL KRTHA3B LGTN LOC162073 LOC387646 LOC401498 LOC642825 KIAA0703 KLC3 KRTHA4 LHB LOC168850 LOC387683 LOC401720 LOC642834 KIAA0748 KLF1 KRTHA8 LHCGR LOC196541 LOC387720 LOC401778 LOC642851 KIAA0773 KLF14 KRTHB1 LHFPL1 LOC197135 LOC387723 LOC401923 LOC642864 KIAA0774 KLF15 KRTHB2 LHFPL3 LOC197322 LOC387762 LOC401945 LOC642948 KIAA0789 KLF17 KRTHB3 LHPP LOC199899 LOC387787 LOC402057 LOC642967 KIAA0804 KLF8 KRTHB4 LHX1 LOC200420 LOC387856 LOC405753 LOC642991 KIAA0853 KLHDC6 KRTHB5 LHX2 LOC200810 LOC387941 LOC407835 LOC643018 KIAA0863 KLHDC7B KRTHB6 LHX3 LOC201229 LOC387978 LOC439914 LOC643045 KIAA1009 KLHDC8A KSP37 LHX4 LOC202134 LOC388135 LOC440005 LOC643075 KIAA1024 KLHL1 KSR2 LHX5 LOC205251 LOC388152 LOC440131 LOC643096 KIAA1033 KLHL14 KY LHX6 LOC221091 LOC388199 LOC440287 LOC643152 KIAA1045 KLHL15 L1CAM LHX8 LOC221711 LOC388272 LOC440338 LOC643176 KIAA1217 KLHL20 L3MBTL LHX9 LOC222699 LOC388282 LOC440419 LOC643185 KIAA1219 KLHL26 L3MBTL4 LIF LOC23117 LOC388335 LOC440836 LOC643225 KIAA1257 KLHL9 LA16c- LILRB2 LOC253012 LOC388407 LOC440863 LOC643273 KIAA1324 KLK1 360B4.1 LILRB3 LOC254028 LOC388419 LOC440925 LOC643355 KIAA1333 KLK10 LACRT LILRB5 LOC254559 LOC388481 LOC440928 LOC643446 KIAA1370 KLK11 LAD1 LIN28 LOC257358 LOC388494 LOC440990 LOC643505 KIAA1411 KLK4 LAIR1 LIN28B LOC283129 LOC388610 LOC441074 LOC643506 KIAA1446 KLK5 LAMA1 LINCR LOC283143 LOC388882 LOC441107 LOC643514 KIAA1529 KMO LAMA3 LIPA LOC283157 LOC388963 LOC441135 LOC643570 KIAA1543 KNDC1 LAMC3 LIPE LOC283340 LOC389151 LOC441177 LOC643659 KIAA1576 KREMEN1 LAMP1 LIX1 LOC283392 LOC389286 LOC441376 LOC643696 KIAA1598 KREMEN2 LARGE LL22NC01- LOC283439 LOC389328 LOC441426 LOC643713 KIAA1622 KRT1 LARS2 81G9.2 LOC283514 LOC389429 LOC441771 LOC643759 KIAA1666 KRT10 LASS4 LMAN1 LOC283999 LOC389458 LOC442208 LOC643785 KIAA1706 KRT12 LAX1 LMAN1L LOC284274 LOC389549 LOC442366 LOC643868 KIAA1715 KRT14 LAYN LMO1 LOC284293 LOC389715 LOC442423 LOC643895 KIAA1718 KRT16 LBH LMO2 LOC284296 LOC389857 LOC494141 LOC643911 KIAA1727 KRT1B LBX1 LMO3 LOC284577 LOC389895 LOC51252 LOC643923 KIAA1729 KRT20 LBX2 LMOD1 LOC284788 LOC390110 LOC541473 LOC643951 KIAA1731 KRT23 LBXCOR1 LMX1A LOC284861 LOC390386 LOC554175 LOC643967

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 LOC643975 LOC645569 LOC653062 LRP1 MAK MED31 MICAL-L2 MRVI1 LOC643982 LOC645574 LOC653091 LRP12 MAK10 MED9 MID1IP1 MS4A8B LOC643988 LOC645643 LOC653104 LRP1B MAL MEGF10 MID2 MSC LOC644019 LOC645648 LOC653200 LRP2 MAL2 MEIS1 MIOX MSI1 LOC644035 LOC645661 LOC653210 LRP3 MALT1 MEIS2 MIP MSL3L1 LOC644058 LOC645722 LOC653222 LRPPRC MAMDC1 MEIS3 MIST MSMB LOC644065 LOC645733 LOC653275 LRRC10 MAMDC2 MEN1 MKI67IP MSRA LOC644072 LOC645776 LOC653299 LRRC15 MAN1C1 MEP1A MKS1 MSRB2 LOC644134 LOC645781 LOC653307 LRRC18 MANBAL MESP1 MKX MST1 LOC644175 LOC645831 LOC653326 LRRC20 MANEAL METRN MLC1 MSX1 LOC644197 LOC645885 LOC653345 LRRC31 MAOB METTL7B MLLT7 MSX2 LOC644224 LOC645902 LOC653353 LRRC35 MAP1B MFAP3L MLN MT1A LOC644225 LOC645914 LOC653357 LRRC3B MAP1D MFAP4 MLNR MT1B LOC644273 LOC645929 LOC653423 LRRC47 MAP3K1 MFRP MLPH MT1F LOC644280 LOC645932 LOC653424 LRRC4C MAP3K7 MFSD2 MLSTD2 MT1G LOC644361 LOC645941 LOC653427 LRRC52 MAP3K7IP2 MFSD4 MLXIPL MT1H LOC644366 LOC645970 LOC653491 LRRC54 MAP3K9 MFSD7 MMD MT1JP LOC644368 LOC645971 LOC653512 LRRC6 MAP4K4 MGAT3 MMD2 MT1M LOC644391 LOC646035 LOC653516 LRRC61 MAP4K5 MGC102966 MMEL1 MT1X LOC644396 LOC646110 LOC653521 LRRC7 MAP6 MGC10955 MMP17 MT3 LOC644403 LOC646129 LOC653621 LRRCC1 MAP7 MGC10993 MMP21 MT4 LOC644500 LOC646192 LOC653625 LRRIQ1 MAPK15 MGC13034 MMP23A MTAC2D1 LOC644541 LOC646215 LOC653630 LRRN1 MAPK8IP2 MGC14436 MMP24 MTAP LOC644558 LOC646220 LOC653673 LRRN5 MAPRE3 MGC15476 MMP25 MTCH1 LOC644572 LOC646248 LOC653680 LRRN6A MAPT MGC16186 MMP28 MTCH2 LOC644672 LOC646451 LOC653686 LRRN6C MARK1 MGC17624 MMP9 MTM LOC644690 LOC646482 LOC653688 LRRN6D MARS MGC24665 MMPL1 MTMR6 LOC644695 LOC646484 LOC653714 LRRTM1 MARVELD3 MGC26356 MN1 MTNR1B LOC644715 LOC646498 LOC653725 LRRTM3 MAS1 MGC26647 MNAT1 MTR LOC644719 LOC646534 LOC653728 LRTM2 MASP1 MGC26733 MOBKL1A MTUS1 LOC644727 LOC646580 LOC653729 LSG1 MASS1 MGC27016 MOBKL2B MTX3 LOC644781 LOC646586 LOC653746 LSM14A MAST1 MGC27348 MOCOS MUC2 LOC644804 LOC646590 LOC653751 LSM14B MATK MGC33486 MOCS1 MUC20 LOC644814 LOC646606 LOC653768 LSP1 MATN1 MGC33530 MOG MUC4 LOC644827 LOC646638 LOC653774 LSR MATN4 MGC33657 MOGAT1 MUC6 LOC644841 LOC646692 LOC653782 LTK MB MGC33846 MOGAT2 MX2 LOC644844 LOC646751 LOC653791 LUZP2 MBIP MGC33926 MOGAT3 MXI1 LOC644862 LOC646831 LOC653807 LUZP4 MBL2 MGC34824 MOP-1 MXRA5 LOC644883 LOC646893 LOC653808 LY6D MBNL3 MGC34829 MORF4L2 MYADM LOC644912 LOC646894 LOC654341 LY6H MBP MGC35023 MOS MYB LOC644980 LOC646948 LOC654433 LYNX1 MC2R MGC35043 MOV10L1 MYBPHL LOC644985 LOC646960 LOC677784 LYPD4 MC5R MGC35212 MOXD1 MYCBPAP LOC645014 LOC646965 LOC81558 LYPD5 MCART2 MGC35295 MPHOSPH6 MYCL1 LOC645029 LOC646987 LOC89944 LYPLA1 MCART6 MGC35308 MPO MYCN LOC645052 LOC646998 LOC90639 LYSMD2 MCCC2 MGC39715 MPP2 MYCNOS LOC645085 LOC647014 LOC91137 LYSMD3 MCF2L MGC42090 MPP6 MYEF2 LOC645104 LOC647041 LOC91461 LYST MCF2L2 MGC42105 MPPED1 MYH11 LOC645113 LOC647042 LOC92196 LYZ MCHR1 MGC42174 MPPED2 MYH14 LOC645187 LOC647043 LOC92691 LYZL4 MCHR2 MGC42367 MRAS MYH7 LOC645191 LOC647044 LOC93622 LYZL6 MCM2 MGC44505 MRE11A MYH7B LOC645249 LOC647054 LONRF1 LZTFL1 MCM6 MGC45438 MRGPRD MYL3 LOC645276 LOC647079 LONRF3 LZTS1 MCOLN2 MGC45491 MRGPRE MYL7 LOC645281 LOC647104 LOR MACF1 MCOLN3 MGC4562 MRGPRF MYO18B LOC645321 LOC647108 LOXHD1 MAD2L2 MCTP1 MGC45800 MRGPRX1 MYO1A LOC645322 LOC647180 LOXL4 MAEL MCTP2 MGC46336 MRGPRX2 MYO1F LOC645335 LOC647219 LPHN1 MAF MDAC1 MGC4825 MRPL46 MYO1H LOC645363 LOC647241 LPL MAFA MDFI MGC50811 MRPL48 MYO3A LOC645379 LOC647251 LPO MAFK MDFIC MGC52282 MRPL55 MYO5B LOC645401 LOC647300 LPPR4 MAG MDGA1 MGC52498 MRPS22 MYO5C LOC645411 LOC647311 LR8 MAGED2 MDM1 MGC5566 MRPS33 MYOCD LOC645444 LOC647353 LRAT MAGED4 MECP2 MGC61571 MRPS6 MYOD1 LOC645469 LOC651362 LRFN5 MAGEF1 MECR MGC99813 MRPS9 MYOG LOC645520 LOC653047 LRIG1 MAGEL2 MED12L MIB1 MRS2L MYOM2

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 MYOZ1 NFAM1 NPM2 NYD-SP14 OR7E91P PARP11 PCSK6 PHF20L1 MYOZ3 NFASC NPNT NYREN18 OR8D2 PARP3 PCSK9 PHF21B MYST2 NFATC2 NPPA OACT5 OR8S1 PARP8 PCTK3 PHF3 MYST3 NFE2 NPPB OAF OR9A2 PARS2 PCYT1B PHF6 MYT1 NFE2L3 NPPC OAS2 OSBPL5 PART1 PDCD4 PHF8 N/A NFIA NPR3 OASL OSM PARVG PDE10A PHGDH NACAL NFKBIE NPTN OAT OSR1 PATE PDE11A PHKA1 NAG NGB NPTX1 OBSL1 OSR2 PAX1 PDE1B PHLDA3 NAG6 NGFR NPTX2 OCA2 OSTalpha PAX2 PDE1C PHOX2A NAGK NGFRAP1L1 NPTXR OCRL OSTM1 PAX3 PDE2A PHOX2B NAGPA NHLH1 NPY ODF1 OTOF PAX4 PDE3B PHYHIP NAGS NHLH2 NPY1R ODF3L1 OTOG PAX5 PDE4A PHYHIPL NALP6 NHS NPY2R OGDHL OTOP1 PAX6 PDE4B PI3 NANOG NID1 NPY5R OLFM1 OTOP2 PAX7 PDE4C PIAS1 NANOGP1 NID2 NR0B1 OLFM2 OTOP3 PAX8 PDE4D PIAS2 NANOS2 NINJ1 NR1I3 OLFML1 OTOR PAX9 PDE4DIP PIB5PA NAP1L2 NINJ2 NR2E1 OLFML2A OTOS PBK PDE6C PIGA NAP1L3 NIP NR4A3 OLIG1 OTP PBX3 PDE7A PIGH NAPA NKAP NR5A1 OLIG2 OTUD1 PCBD1 PDE8B PIGM NAPB NKD1 NR5A2 OLIG3 OTUD6A PCBP3 PDE9A PIK3AP1 NAPG NKD2 NR6A1 OMA1 OTX1 PCDH1 PDGFRA PIK3C3 NAPSA NKX1-1 NRBF2 ONECUT1 OTX2 PCDH19 PDGFRB PIK3CD NAT11 NKX2-2 NRD1 ONECUT2 OVGP1 PCDH20 PDHA1 PIK3R5 NAV2 NKX2-3 NRG1 OPCML OVOL1 PCDH21 PDIA5 PIN4 NBL1 NKX2-5 NRG2 OPN1LW OVOL2 PCDH8 PDIA6 PINX1 NBLA00301 NKX2-6 NRG3 OPN1SW OXCT2 PCDHA13 PDRG1 PIP5K1B NBPF11 NKX2-8 NRG4 OPN5 OXGR1 PCDHAC1 PDYN PIP5K2A NBPF3 NKX3-1 NRGN OPRD1 OXT PCDHAC2 PDZD2 PIP5K2C NBPF9 NKX6-1 NRIP3 OPRK1 OXTR PCDHB1 PDZD3 PIPOX NCALD NKX6-2 NRK OPRL1 P2RX2 PCDHB13 PDZD4 PIR NCAM1 NLE1 NRL OPTC P2RX3 PCDHB2 PDZD8 PITPNM3 NCF4 NMBR NRN1 OR10G3 P2RX5 PCDHB8 PDZK1 PITRM1 NCK2 NME6 NRXN1 OR10P1 P2RY4 PCDHGA1 PDZRN3 PITX1 NCKAP1 NMNAT2 NRXN2 OR13A1 P2RY6 PCDHGA10 PEBP4 PITX2 NCKAP1L NMU NRXN3 OR13H1 P2RY8 PCDHGA11 PENK PITX3 NCOA2 NOC3L NSF OR13J1 P518 PCDHGA12 PEPD PIWIL3 NCOA5 NOG NT5C1A OR1F1 PABPC3 PCDHGA2 PEPP-2 PJA1 NCOA7 NOL10 NT5E OR1J1 PABPC5 PCDHGA3 PEX10 PKD1L2 NCR2 NOL4 NTF3 OR1J2 PACSIN1 PCDHGA4 PEX12 PKD2L1 NDFIP2 NOM1 NTF5 OR1J4 PADI1 PCDHGA5 PEX16 PKDREJ NDP NOPE NTN1 OR1L4 PADI2 PCDHGA6 PEX5L PKHD1 NDRG2 NOS1 NTN2L OR1L6 PAK1 PCDHGA7 PEX7 PKIB NDUFA4 NOS2A NTNG1 OR2AG2 PAK3 PCDHGA8 PFDN4 PKLR NDUFA4L2 NOTCH3 NTNG2 OR2AT4 PAK4 PCDHGA9 PFKFB1 PKP1 NDUFB11 NOTO NTRK1 OR2K2 PAK6 PCDHGB1 PFKFB2 PKP2 NEF3 NOTUM NTRK2 OR2V2 PAK7 PCDHGB2 PFKFB3 PKP3 NEFH NOVA1 NTRK3 OR2Y1 PALM2 PCDHGB3 PFN3 PLA2G2A NEFL NOVA2 NUAK2 OR4D1 PANK1 PCDHGB4 Pfs2 PLA2G2D NELL1 NOX5 NUBPL OR4D10 PANK3 PCDHGB5 PGAM2 PLA2G2F NELL2 NOXO1 NUCKS1 OR4D2 PANX3 PCDHGB6 PGAM4 PLA2G3 NENF NP NUDCD3 OR4F21 PAP2D PCDHGB7 PGBD2 PLA2G4E NEO1 NPAL2 NUDT10 OR4F3 PAPD1 PCGF1 PGC PLA2G5 NES NPAS1 NUDT8 OR52B2 PAPOLA PCGF3 PGK1 PLA2G7 NETO1 NPAS3 NUP210 OR52B6 PAPOLB PCGF4 PGLYRP2 PLAC2 NEURL NPAS4 NUPL1 OR52H1 PAPOLG PCID1 PGLYRP3 PLAGL1 NEUROD1 NPBWR1 NXF2 OR52W1 PAQR3 PCK2 PGR PLB1 NEUROD2 NPBWR2 NXF4 OR56A1 PAQR4 PCMTD2 PHACS PLCB2 NEUROD4 NPC1L1 NXF5 OR56B4 PAQR5 PCOLCE2 PHACTR1 PLCE1 NEUROD6 NPFFR1 NXPH1 OR5AU1 PAQR9 PCP2 PHACTR3 PLCG2 NEUROG1 NPFFR2 NXPH2 OR5B21 PARD6B PCP4L1 PHB PLCH1 NEUROG2 NPHS1 NXPH3 OR6C4 PARD6G PCSK1 PHF14 PLCH2 NEUROG3 NPHS2 NXPH4 OR6S1 PARL PCSK1N PHF15 PLCXD3 NF2 NPL NXT2 OR6V1 PARP1 PCSK2 PHF2 PLD5

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 PLEKHB1 PPL PROZ PTPN20B raptor RGL3 RORA RP13- PLEKHG3 PPM1B PRP2 PTPN3 RARG RGMA RORB 360B22.2 PLEKHG6 PPM1E PRPF18 PTPN5 RARRES2 RGN RORC RP13- PLEKHH1 PPP1R14A PRPF19 PTPRM RASA3 RGPD5 RP1 383K5.1 PLP1 PPP1R14C PRPH PTPRN RASAL1 RGS10 RP11- RP13- PLRG1 PPP1R14D PRPS1 PTPRO RASD2 RGS14 114H20.1 383K5.2 PLS3 PPP1R1A PRR10 PTPRR RASEF RGS16 RP11- RP1-93H18.5 PPP1R1B PRR5 PTPRT RASGEF1A RGS18 119E20.1 RP3- PLXDC1 PPP1R2 PRR8 PTPRU RASGEF1C RGS20 RP11- 355C18.2 PLXDC2 PPP1R2P1 PRRG4 PTPRZ1 RASGRF1 RGS22 130N24.1 RP3-473B4.1 PLXNA1 PPP2R2B PRRT1 PUNC RASGRF2 RGS6 RP11-13E5.1 RP3-474I12.5 PLXNA2 PPP2R2C PRRX2 PURA RASGRP1 RGS8 RP11- RP3-510O8.5 PLXNA4B PPP2R3B PRSS1 PUS3 RASGRP2 RGS9 145H9.1 RP4- PLXNC1 PPP2R5C PRSS12 PUS7L RASGRP4 RGS9BP RP11- 621O15.2 PMFBP1 PPP5C PRSS16 PVALB RASL10B RHBDL3 159H20.4 RP4-692D3.1 PMP22 PPT1 PRSS2 PWP1 RASL11B RHBG RP11- RP4- PMPCB PPY PRSS21 PXT1 RASL12 RHCG 165H20.1 742C19.3 PNCK PPYR1 PRSS22 PYCARD RASSF2 RHEB RP11- RP5- PNMA3 PRAC PRSS3 PYDC1 RASSF5 RHOBTB2 262D11.5 1103G7.6 PNMA5 PRAP1 PRSS33 PYY RAX RHOU RP11- RP5- PNMT PRDM1 PRSS36 PYY2 RB1CC1 RHPN1 262H14.4 1153D9.3 PNOC PRDM11 PRTN3 PZP RBBP7 RIBC1 RP1-127L4.6 RP5- PNPLA1 PRDM12 PSCD4 QSCN6L1 RBL2 RIC3 RP11- 821D11.2 PNPLA5 PRDM13 PSD R7BP RBM10 RIMS1 308B5.5 RP5- PNPT1 PRDM14 PSD2 RAB11FIP2 RBM17 RIMS2 RP11- 860F19.3 PODN PRDM15 PSD4 RAB11FIP4 RBM19 RIMS3 321G1.1 RP6- PODXL2 PRDM16 PSKH2 RAB12 RBM28 RIMS4 RP11- 213H19.1 POGZ PRDM4 PSMA1 RAB15 RBM35A RIOK1 34P13.4 RP6- POLA PRDM8 PSMA5 RAB18 RBMX2 RIOK3 RP11- 213H19.2 POLG PRELP PSMB2 RAB19B RBP2 RIPK4 350O14.10 RP9 POLR3B PREP PSMD1 RAB20 RBP4 RIPK5 RP11- RPESP POM121L2 PRF1 PSMD11 RAB21 RBP7 RIS1 35F15.2 RPIB9 POMC PRG2 PSMD14 RAB28 RBPSUHL RLBP1 RP11-35N6.1 RPIP8 PON1 PRG3 PSMD2 RAB37 RCOR2 RLF RP11- RPL38 PON3 PRIMA1 PSMD6 RAB39 RCSD1 RLN2 378J18.4 RPL6 POP5 PRKAB1 PSMD8 RAB39B RDH12 RLN3 RP11- RPL8 POP7 PRKACG PSME3 RAB3B RDH13 RNASE11 393H10.2 RPN1 POU1F1 PRKAG3 PSMF1 RAB3C RDH16 RNASE13 RP11- RPP14 POU2F3 PRKCB1 PTAR1 RAB3GAP1 RDH8 RNASE2 410N8.4 RPP30 POU3F1 PRKCG PTCH RAB3GAP2 RDHE2 RNASE3 RP11- RPRM POU3F2 PRKCZ PTCHD1 RAB3IL1 RDM1 RNASE6 444E17.2 RPRML POU3F3 PRKD1 PTCHD3 RAB6A REEP1 RNASE7 RP11- RPS10 POU3F4 PRKD2 PTD004 RAB6B REEP4 RNASE8 444E17.5 RPS17 POU4F1 PRKG1 PTENP1 RAB6C REG3G RNASEL RP11- RPS19 POU4F2 PRLHR PTER RAB7 REG4 RND1 450P7.3 RPS20 POU4F3 PRLR PTF1A RAB7L1 RELN RND2 RP11-484I6.3 RPS3A PP8961 PRM1 PTGDR RAB9B REM1 RNF128 RP11- RPS4X PPAP2C PRM3 PTGER2 RABAC1 REPIN1 RNF130 493K23.2 RPS6KA1 PPAPDC1A PRMT2 PTGER3 RABL5 REPS2 RNF150 RP11- RPS6KA3 PPAPDC3 PRMT6 PTGFR RACGAP1 RERG RNF165 506K6.3 RPS6KC1 PPARG PRMT8 PTGIS RAD18 RESP18 RNF175 RP1- RPTN PPARGC1B PRND PTH RAD23B RET RNF180 172B20.3 RRAD PPFIA1 PRNP PTHR1 RAET1G RETN RNF182 RP1-29C18.7 RRAGD PPFIA2 PRNPIP PTHR2 RAET1L REV1L RNF187 RP13- RRBP1 PPFIA3 PRNT PTK2B RAG1 RFP RNF32 102H20.1 RRP22 PPFIA4 PROK1 PTK7 RAI2 RFPL1 RNF39 RP13-11B7.1 RSAD1 PPFIBP2 PROK2 PTK9 RALB RFPL2 RNF43 RP13- RSPO1 PPIAL4 PROKR1 PTN RAMP1 RFX3 RNF6 153N15.1 RSPO2 PPID PROKR2 PTP4A1 RAMP3 RFX4 ROBO2 RP13- RSPO4 PPIE PROM1 PTP4A3 RANBP5 RFXDC1 ROBO3 15M17.2 RSRC1 PPIG PROP1 PTPLAD2 RAP1GAP RG9MTD1 ROPN1 RP1-32F7.2 RSU1 PPIH ProSAPiP1 PTPN11 RAP2C RGAG1 ROPN1B RP13- RTBDN PPIL4 PROX1 PTPN18 RAPGEF4 RGAG4 ROR2 347D8.3 RTKN

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 RTN1 SCNN1B SFT2D1 SLC17A7 SLC43A2 SNAG1 SPRY3 SUHW3 RTN2 SCNN1G SFTPC SLC18A1 SLC43A3 SNAI2 SPSB4 SULT1A1 RTN4RL1 SCP2 SFTPD SLC18A2 SLC44A3 SNAP25 SPTB SULT1A2 RTN4RL2 SCPEP1 SFTPG SLC18A3 SLC45A2 SNAP91 SPTBN4 SULT1C1 RTP2 SCRT1 SFXN1 SLC1A2 SLC4A1 SNCB SPTLC1 SULT4A1 RTTN SCRT2 SGCB SLC1A3 SLC4A10 SND1 SPTLC2 SUMF1 RUNX1 SCT SGOL2 SLC1A6 SLC4A3 SNFT SPTY2D1 SUNC1 RUNX2 SCTR SGPL1 SLC22A11 SLC4A4 SNIP SRGAP3 SUPT3H RUNX3 SCUBE1 SGPP2 SLC22A12 SLC4A5 SNTB1 SRP46 SUSD4 RUTBC2 SCUBE2 SH2D3A SLC22A15 SLC5A1 SNTG2 SRPRB SUV420H1 RUVBL1 SCUBE3 SH2D4A SLC22A16 SLC5A10 SNX12 SRPX2 SV2A RXFP3 SCYL3 SH3BGR SLC22A17 SLC5A5 SNX16 SRRM1 SV2B RXRG SDAD1 SH3GL2 SLC22A18 SLC5A8 SNX22 SRrp35 SV2C RYBP SDC1 SH3GL3 SLC22A3 SLC6A1 SOCS2 SRXN1 SVOP RYR1 SDC2 SH3KBP1 SLC22A6 SLC6A11 SOCS6 SSFA2 SYAP1 RYR2 SDK1 SHANK1 SLC22A7 SLC6A12 SOD3 SSPN SYBL1 RYR3 SEC11L3 SHANK2 SLC22A8 SLC6A13 SOLH SST SYCP1 S100A10 SEC13L1 SHC4 SLC24A2 SLC6A15 SORBS3 SSTR2 SYCP2 S100A11 SEC14L3 SHD SLC24A3 SLC6A17 SORCS2 SSTR3 SYK S100A7 SEC14L4 SHF SLC24A4 SLC6A2 SORCS3 SSTR4 SYN2 S100B SEC23IP SHFM1 SLC25A23 SLC6A20 SORL1 SSU72 SYN3 S100G SEC61A1 SHH SLC25A24 SLC6A3 SORT1 ST14 SYNGAP1 SAA3P SEC61G SHMT2 SLC25A28 SLC6A4 SOST ST6GAL2 SYNGR3 SAA4 SEC63 SHOX SLC25A36 SLC6A5 SOX1 ST6GALNAC SYNJ2BP SAAL1 SELK SHOX2 SLC25A4 SLC6A6 SOX11 5 SYNPO SALL1 SELV SHPRH SLC26A1 SLC6A7 SOX14 ST7 SYNPO2L SALL2 SEMA3B SHRM SLC26A10 SLC6A8 SOX2 ST8SIA1 SYNPR SALL3 SEMA3C SIDT1 SLC26A3 SLC6A9 SOX21 ST8SIA2 SYP SAMD11 SEMA3E SIGLEC1 SLC26A4 SLC7A1 SOX3 ST8SIA3 SYPL2 SAMD3 SEMA4F SIGLEC12 SLC26A5 SLC7A10 SOX5 ST8SIA5 SYT10 SAMD7 SEMA5B SIGLEC5 SLC26A9 SLC7A14 SOX8 STAB2 SYT12 SAPS3 SEMA6A SIGLEC6 SLC27A2 SLC7A3 SOX9 STAC2 SYT13 SARS SEMA7A SIGLEC8 SLC27A6 SLC7A4 SP5 STAC3 SYT14 SATB1 SENP1 SIGLEC9 SLC29A2 SLC7A5 SP6 STAM2 SYT15 SATB2 SEPHS1 SIM1 SLC29A3 SLC8A2 SP7 STARD8 SYT16 SBF1 SEPX1 SIM2 SLC2A10 SLC9A2 SP8 STAT6 SYT17 SBK1 SERGEF SIRPA SLC2A14 SLC9A3 SPA17 STC1 SYT2 SBSN SERHL SIRPG SLC2A4 SLC9A4 SPAG6 STC2 SYT3 SC5DL SERHL2 SIRT5 SLC2A6 SLCO2B1 SPATA18 STEAP3 SYT5 SCAMP2 SERINC2 SIX1 SLC2A9 SLCO3A1 SPATA20 STEAP4 SYT6 SCAMP5 SERPINA13 SIX2 SLC30A10 SLCO5A1 SPATA3 STIM2 SYT7 SCAND1 SERPINB10 SIX3 SLC30A2 SLFN13 SPATA5L1 STK11IP SYT9 SCAP2 SERPINB5 SIX4 SLC30A3 SLFN5 SPATA7 STK17B SYTL4 SCARA5 SERPINB7 SIX6 SLC30A8 SLIT3 SPATC1 STK3 SYVN1 SCD5 SERPINB8 SLAMF8 SLC31A2 SLITRK3 SPATS1 STK31 T SCFD1 SERPINC1 SLAMF9 SLC32A1 SLITRK5 SPBC25 STK32A TAAR9 SCFD2 SERPING1 SLC10A1 SLC34A1 SLN SPECC1 STK32B TAB3 SCG3 SETBP1 SLC10A4 SLC34A2 SLPI SPERT STK32C TAC1 SCGB1D1 SETD4 SLC11A1 SLC35B4 SMAD2 SPESP1 STK33 TAC3 SCGB1D2 SETD7 SLC12A1 SLC35D2 SMAF1 SPFH2 STK35 TACC3 SCGB1D4 SEZ6 SLC12A5 SLC35D3 SMAP1L SPG3A STMN2 TACR1 SCGB2A1 SEZ6L SLC12A8 SLC35F1 SMARCA2 SPIB STMN4 TACR2 SCGB2A2 SEZ6L2 SLC13A3 SLC35F2 SMARCAL1 SPINK1 STOX2 TACR3 SCGB3A1 SF3B5 SLC13A5 SLC35F3 SMARCD3 SPINK2 STRA6 TADA1L SCIN SFRP1 SLC14A1 SLC36A2 SMC1L1 SPINK4 STT3B TAF1B SCML4 SFRP2 SLC14A2 SLC36A4 SMC2L1 SPINT1 STX1B2 TAF1L SCN10A SFRP4 SLC15A1 SLC37A3 SMCX SPO11 STX7 TAF4 SCN11A SFRP5 SLC15A3 SLC38A3 SMOC1 SPOCK1 STXBP2 TAF7 SCN2B SFRS1 SLC16A10 SLC38A4 SMOC2 SPOCK2 STXBP3 TAGAP SCN3B SFRS12 SLC16A12 SLC38A5 SMPDL3B SPOCK3 STXBP5L TAL2 SCN4B SFRS15 SLC16A2 SLC39A11 SMPX SPON1 STXBP6 TARBP1 SCN5A SFRS6 SLC16A9 SLC39A3 SMUG1 SPON2 SUHW1 TAS1R1 SCN7A SFRS8 SLC17A6 SLC3A1 SMYD1 SPR SUHW2 TAS1R2

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 TAS2R40 TFAP2B TMEM132E TNNT3 TRUB1 UBLCP1 VAV3 WNT7B TAS2R7 TFAP2C TMEM141 TNP1 TSC22D3 UBR2 VAX1 WNT8B TASP1 TFAP2D TMEM145 TNP2 TSCOT UBXD3 VAX2 WNT9A TAT TFAP2E TMEM16A TNRC6B TSGA10 UCHL1 VDR WNT9B TBC1D1 TFCP2L1 TMEM16C TNRC9 TSGA13 UCN VENTX WRB TBC1D12 TFDP1 TMEM16D TNS1 TSHR UCN3 VGF WRN TBC1D19 TFEB TMEM16E TNS3 TSHZ2 UCP1 VGLL2 WT1 TBC1D21 TFEC TMEM16J TNS4 TSHZ3 UGCGL2 VGLL3 WWC1 TBC1D3 TFF3 TMEM20 TNT TSLP UGT3A2 VIPR1 WWP2 TBC1D3B TGFA TMEM23 TNXB TSNARE1 UGT8 VLDLR XAGE3 TBCA TGFBI TMEM27 TOLLIP TSP50 ULBP1 VMD2 XCR1 TBL1X TGM3 TMEM28 TOM1L1 TSPAN17 ULBP2 VMD2L1 XKR5 TBPL1 TGM5 TMEM29 TOP1 TSPAN19 ULBP3 VPREB1 XKR6 TBR1 TGM7 TMEM31 TOP3B TSPAN3 UMODL1 VPS13A XKR7 TBRG1 THBS2 TMEM33 TP73 TSPAN31 UMPS VPS18 XPNPEP2 TBRG4 THBS4 TMEM34 TPBG TSPAN33 UNC45B VPS37D XPO4 TBX1 THEG TMEM35 TPCN2 TSPAN6 UNC5A VPS4B XPR1 TBX15 THEM4 TMEM37 TPD52L1 TSPYL2 UNC5B VSIG1 XRCC5 TBX2 THOC3 TMEM45A TPH1 TTBK1 UNC5C VSNL1 XRN2 TBX20 THPO TMEM45B TPH2 TTC1 UNC5CL VSX1 XYLT1 TBX21 THRAP6 TMEM5 TPM3 TTC14 UNC5D VTCN1 XYLT2 TBX3 THRB TMEM55B TPPP TTC18 UNG2 VWA2 YBX2 TBX4 THSD3 TMEM56 TPRX1 TTC22 UNQ3033 WAS YEATS4 TBX5 THTPA TMEM61 TPSD1 TTC24 UNQ467 WASF3 YIPF7 TBXAS1 THY1 TMEM63C TRA@ TTC8 UNQ470 WBSCR17 YKT6 TCBA1 TIAM1 TMEM65 TRA16 TTC9B UNQ473 WDR17 YOD1 TCEAL1 TIGD2 TMEM67 TRAJ17 TTK UNQ5831 WDR21A YPEL1 TCEAL2 TIMM10 TMEM71 TRAM1 TTLL10 UNQ6411 WDR31 YPEL4 TCEAL3 TIMP3 TMEM74 TRBC1 TTLL12 UNQ6488 WDR5 YPEL5 TCEAL4 TIMP4 TMEM86A TRBV21-1 TTLL6 UNQ6490 WDR60 YSK4 TCEAL5 TINAG TMEM9 TREH TTLL9 UNQ739 WDR61 YTHDC2 TCEAL6 TIP39 TMEM92 TRH TTMA UNQ9433 WDR63 YTHDF1 TCEAL8 TITF1 TMEM97 TRHDE TTPA UPF1 WDR72 YTHDF3 TCERG1 TJAP1 TMOD1 TRIM10 TTR UPF2 WDR78 YWHAB TCERG1L TJP3 TMOD2 TRIM15 TTYH1 UPK1B WDR8 YY1 TCF15 TLL2 TMPRSS13 TRIM17 TTYH2 UPK2 WDTC1 ZAK TCF2 TLN2 TMPRSS3 TRIM29 TUB UPK3A WFDC1 ZAN TCF21 TLOC1 TMPRSS6 TRIM31 TUBA4 UPP2 WFDC12 ZAP70 TCF23 TLR10 TMPRSS9 TRIM33 TUBB2B UROC1 WFDC2 ZAR1 TCF3 TLR2 TMSL8 TRIM36 TUBB4 USF2 WFDC5 ZBED4 TCHHL1 TLR5 TMTC1 TRIM40 TUBGCP3 USH1C WFDC6 ZBTB12 TCL1A TLX1 TNC TRIM45 TULP1 USH1G WFIKKN2 ZBTB16 TCOF1 TLX2 TNFAIP8 TRIM54 TUSC1 USH3A WFS1 ZBTB34 TCP10L TLX3 TNFRSF11B TRIM59 TUSC5 USP1 WIF1 ZBTB40 TDH TM4SF5 TNFRSF13B TRIM63 TWIST1 USP11 WISP1 ZBTB43 TDP1 TM6SF2 TNFRSF13C TRIM67 TWISTNB USP14 WIT1 ZBTB7C TDRD1 TM9SF4 TNFRSF17 TRIM71 TXN USP2 WNK2 ZBTB8 TDRD5 TMC2 TNFRSF18 TRIM72 TXNDC USP22 WNK3 ZCCHC12 TDRD7 TMCC2 TNFRSF19 TRIM9 TXNDC2 USP26 WNK4 ZCCHC13 TEAD3 TMCO5 TNFRSF8 TRIO TXNDC6 USP44 WNT1 ZCCHC16 TEDDM1 TMED3 TNFRSF9 TRIP12 TXNL2 USP47 WNT10A ZCCHC6 TEF TMED9 TNFSF11 TRIT1 UBE1 USP6 WNT10B ZD52F10 TEKT1 TMEM100 TNFSF14 TRMT12 UBE2D1 USP9X WNT11 ZDHHC1 TEKT3 TMEM103 TNFSF7 TRPA1 UBE2E2 UST WNT16 ZDHHC13 TERF1 TMEM105 TNFSF8 TRPC3 UBE2E3 UTF1 WNT2 ZDHHC19 TERT TMEM106B TNFSF9 TRPC5 UBE2I UTP11L WNT2B ZDHHC22 TESC TMEM106C TNIP3 TRPC6 UBE2L6 UTP20 WNT3 ZDHHC8 TESSP5 TMEM108 TNK1 TRPM2 UBE2Q1 UTS2R WNT3A ZDHHC9 TEX13A TMEM109 TNKS TRPM3 UBE2Q2 UXS1 WNT4 ZFAND3 TEX13B TMEM117 TNMD TRPM4 UBE2S VAMP8 WNT5A ZFHX4 TEX2 TMEM126A TNNI1 TRPM8 UBE2V2 VANGL1 WNT5B ZFP37 TF TMEM126B TNNI3 TRPV5 UBL3 VANGL2 WNT6 ZFP90 TFAP2A TMEM130 TNNT1 TRPV6 UBL4B VAPA WNT7A ZG16

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 ZHX2 ZNF692 ZIC1 ZNF703 ZIC2 ZNF706 ZIC3 ZNF74 ZIC4 ZNF746 ZIC5 ZNF80 ZKSCAN1 ZNFN1A1 ZMAT1 ZNFN1A2 ZMAT4 ZNFN1A3 ZMYND15 ZNHIT4 ZNF11B ZP3 ZNF131 ZPBP2 ZNF167 ZW10 ZNF175 ZNF179 ZNF187 ZNF19 ZNF195 ZNF204 ZNF206 ZNF222 ZNF223 ZNF239 ZNF263 ZNF265 ZNF283 ZNF287 ZNF292 ZNF30 ZNF312 ZNF322A ZNF326 ZNF330 ZNF334 ZNF335 ZNF343 ZNF358 ZNF365 ZNF406 ZNF423 ZNF452 ZNF462 ZNF485 ZNF488 ZNF498 ZNF507 ZNF510 ZNF517 ZNF533 ZNF541 ZNF543 ZNF574 ZNF618 ZNF651 ZNF655 ZNF658 ZNF659 ZNF660 ZNF662 ZNF663 ZNF678 ZNF683 ZNF687

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Table S4: Ezh2 target genes

Accession # Gene ID Sequence Description Fold Change NM_004114 FGF13 Homo sapiens fibroblast growth factor 13 (FGF13), transcript variant 1, mRNA [NM_004114] 15.6 NM_003108 SOX11 Homo sapiens SRY (sex determining region Y)-box 11 (SOX11), mRNA [NM_003108] 7.9 NM_002825 PTN Homo sapiens pleiotrophin (PTN), mRNA [NM_002825] 7.5 AK090803 SRrp35 Homo sapiens serine-arginine repressor protein (35 kDa) (SRrp35), mRNA [NM_080743] 7.0 NM_018644 B3GAT1 Homo sapiens beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 2, mRNA [NM_054025] 6.8 Downloaded from NM_000237 LPL Homo sapiens lipoprotein lipase (LPL), mRNA [NM_000237] 6.8 NM_000599 IGFBP5 Homo sapiens insulin-like growth factor binding protein 5 (IGFBP5), mRNA [NM_000599] 6.6 NM_152728 ENST00000323355 Uncharacterized protein C18orf20 Precursor [Source:UniProtKB/Swiss-Prot;Acc:Q7Z4B0] [ENST00000323355] 6.0 AL136578 TMEM108 Homo sapiens transmembrane protein 108 (TMEM108), transcript variant 1, mRNA [NM_023943] 5.8 NM_000885 ITGA4 Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4), mRNA [NM_000885] 5.8

http://hyper.ahajournals.org/ NM_000800 FGF1 Homo sapiens fibroblast growth factor 1 (acidic) (FGF1), transcript variant 1, mRNA [NM_000800] 5.6 NM_018936 PCDHB2 Homo sapiens protocadherin beta 2 (PCDHB2), mRNA [NM_018936] 5.1 NM_022893 BCL11A Homo sapiens B-cell CLL/lymphoma 11A (zinc finger protein) (BCL11A), transcript variant 1, mRNA [NM_022893] 4.7 AK075558 C1orf187 Homo sapiens cDNA PSEC0258 fis, clone NT2RP3003731. [AK075558] 4.6 NM_005382 NEFM Homo sapiens neurofilament, medium polypeptide (NEFM), transcript variant 1, mRNA [NM_005382] 4.5 BC008690 JPH3 Homo sapiens junctophilin 3, mRNA (cDNA clone IMAGE:3867947), complete cds. [BC008690] 4.5 NM_178012 TUBB2B Homo sapiens tubulin, beta 2B (TUBB2B), mRNA [NM_178012] 4.4 BC044246 TMEM200A Homo sapiens transmembrane protein 200A, mRNA (cDNA clone MGC:50847 IMAGE:5760073), complete cds. [BC044246] 4.4

byguest onSeptember11, 2012 NM_004385 VCAN Homo sapiens versican (VCAN), transcript variant 1, mRNA [NM_004385] 4.3 NM_025212 CXXC4 Homo sapiens CXXC finger 4 (CXXC4), mRNA [NM_025212] 4.3 BC035246 RIMBP3 Homo sapiens RIMS binding protein 3 (RIMBP3), mRNA [NM_015672] 4.3 NM_030926 ITM2C Homo sapiens integral membrane protein 2C (ITM2C), transcript variant 1, mRNA [NM_030926] 4.2 NM_004086 COCH Homo sapiens coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2, mRNA [NM_004086] 4.2 NM_015689 DENND2A Homo sapiens DENN/MADD domain containing 2A (DENND2A), mRNA [NM_015689] 4.2 NM_030955 ADAMTS12 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif, 12 (ADAMTS12), mRNA [NM_030955] 4.2 NM_152679 SLC10A4 Homo sapiens solute carrier family 10 (sodium/bile acid cotransporter family), member 4 (SLC10A4), mRNA [NM_152679] 4.1 NM_053036 NPFFR2 Homo sapiens neuropeptide FF receptor 2 (NPFFR2), transcript variant 2, mRNA [NM_053036] 4.1 AB011103 KIF5C Homo sapiens kinesin family member 5C (KIF5C), mRNA [NM_004522] 4.1 NM_012445 SPON2 Homo sapiens spondin 2, extracellular matrix protein (SPON2), transcript variant 1, mRNA [NM_012445] 4.0 NM_198521 C12orf42 Homo sapiens chromosome 12 open reading frame 42 (C12orf42), transcript variant 1, mRNA [NM_198521] 4.0 NM_012259 HEY2 Homo sapiens hairy/enhancer-of-split related with YRPW motif 2 (HEY2), mRNA [NM_012259] 4.0 NM_021021 SNTB1 Homo sapiens syntrophin, beta 1 (dystrophin-associated protein A1, 59kDa, basic component 1) (SNTB1), mRNA [NM_021021] 4.0 AK056142 BEAN Homo sapiens brain expressed, associated with Nedd4 (BEAN), mRNA [NM_001136106] 4.0 NM_005715 UST Homo sapiens uronyl-2-sulfotransferase (UST), mRNA [NM_005715] 3.9 NM_052913 TMEM200A Homo sapiens transmembrane protein 200A (TMEM200A), mRNA [NM_052913] 3.9 NM_000625 NOS2 Homo sapiens nitric oxide synthase 2, inducible (NOS2), mRNA [NM_000625] 3.9 NM_002521 NPPB Homo sapiens natriuretic peptide precursor B (NPPB), mRNA [NM_002521] 3.8 NM_022137 SMOC1 Homo sapiens SPARC related modular calcium binding 1 (SMOC1), transcript variant 1, mRNA [NM_001034852] 3.7 NM_001299 CNN1 Homo sapiens calponin 1, basic, smooth muscle (CNN1), mRNA [NM_001299] 3.7 NM_002523 NPTX2 Homo sapiens neuronal pentraxin II (NPTX2), mRNA [NM_002523] 3.6 NM_003068 SNAI2 Homo sapiens snail homolog 2 (Drosophila) (SNAI2), mRNA [NM_003068] 3.5 NM_153350 FBXL16 Homo sapiens F-box and leucine-rich repeat protein 16 (FBXL16), mRNA [NM_153350] 3.4 NM_005544 IRS1 Homo sapiens insulin receptor substrate 1 (IRS1), mRNA [NM_005544] 3.4 NM_002025 AFF2 Homo sapiens AF4/FMR2 family, member 2 (AFF2), mRNA [NM_002025] 3.4 NM_024888 PRG2 Homo sapiens plasticity-related gene 2 (PRG2), mRNA [NM_024888] 3.4 Downloaded from NM_138711 PPARG Homo sapiens peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 3, mRNA [NM_138711] 3.4 NM_003236 TGFA Homo sapiens transforming growth factor, alpha (TGFA), transcript variant 1, mRNA [NM_003236] 3.4 NM_181538 GJC3 Homo sapiens gap junction protein, gamma 3, 30.2kDa (GJC3), mRNA [NM_181538] 3.3 NM_001874 CPM Homo sapiens carboxypeptidase M (CPM), transcript variant 1, mRNA [NM_001874] 3.3 NM_013305 ST8SIA5 Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (ST8SIA5), mRNA [NM_013305] 3.3

http://hyper.ahajournals.org/ NM_003956 CH25H Homo sapiens cholesterol 25-hydroxylase (CH25H), mRNA [NM_003956] 3.3 AK127778 CXXC4 CXXC-type zinc finger protein 4 (Inhibition of the Dvl and axin complex protein) [Source:UniProtKB/Swiss-Prot;Acc:Q9H2H0] [ENST00000305749] 3.3 NM_018406 MUC4 Homo sapiens mucin 4, cell surface associated (MUC4), transcript variant 1, mRNA [NM_018406] 3.3 NM_198545 C1orf187 Homo sapiens chromosome 1 open reading frame 187 (C1orf187), mRNA [NM_198545] 3.3 NM_000488 SERPINC1 Homo sapiens serpin peptidase inhibitor, clade C (antithrombin), member 1 (SERPINC1), mRNA [NM_000488] 3.2 BX537946 EPHA5 Homo sapiens mRNA; cDNA DKFZp686C0686 (from clone DKFZp686C0686); complete cds. [BX537946] 3.2 NM_022123 NPAS3 Homo sapiens neuronal PAS domain protein 3 (NPAS3), transcript variant 1, mRNA [NM_022123] 3.2 NM_003507 FZD7 Homo sapiens frizzled homolog 7 (Drosophila) (FZD7), mRNA [NM_003507] 3.2 NM_014867 KBTBD11 Homo sapiens kelch repeat and BTB (POZ) domain containing 11 (KBTBD11), mRNA [NM_014867] 3.2 byguest onSeptember11, 2012 NM_145306 C10orf35 Homo sapiens chromosome 10 open reading frame 35 (C10orf35), mRNA [NM_145306] 3.2 NM_015381 FAM19A5 Homo sapiens family with sequence similarity 19 (chemokine (C-C motif)-like), member A5 (FAM19A5), transcript variant 2, mRNA [NM_015381] 3.1 NM_000641 IL11 Homo sapiens interleukin 11 (IL11), mRNA [NM_000641] 3.1 NM_017549 EPDR1 Homo sapiens ependymin related protein 1 (zebrafish) (EPDR1), mRNA [NM_017549] 3.1 AK023723 HS3ST3B1 Heparan sulfate glucosamine 3-O-sulfotransferase 3B1 (EC 2.8.2.30)(Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1)(Heparan sulfate 3-O-sulfotra 3.1 AF199613 FGF13 Homo sapiens fibroblast growth factor 13 (FGF13), transcript variant 4, mRNA [NM_001139498] 3.1 NM_016651 DACT1 Homo sapiens dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) (DACT1), transcript variant 1, mRNA [NM_016651] 3.1 NM_030820 COL21A1 Homo sapiens collagen, type XXI, alpha 1 (COL21A1), mRNA [NM_030820] 3.0 NM_006909 RASGRF2 Homo sapiens Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2), mRNA [NM_006909] 3.0 NM_015686 FAM155B Homo sapiens family with sequence similarity 155, member B (FAM155B), mRNA [NM_015686] 3.0 NM_138415 PHF21B Homo sapiens PHD finger protein 21B (PHF21B), transcript variant 1, mRNA [NM_138415] 3.0 NM_001018084 SLC26A10 Homo sapiens cDNA FLJ16735 fis, clone BRACE2005457, weakly similar to PENDRIN. [AK122981] 3.0 AK055101 C6orf168 Homo sapiens chromosome 6 open reading frame 168 (C6orf168), mRNA [NM_032511] 3.0 NM_181482 C18orf1 Homo sapiens chromosome 18 open reading frame 1 (C18orf1), transcript variant a2, mRNA [NM_181482] 3.0 NM_004538 NAP1L3 Homo sapiens nucleosome assembly protein 1-like 3 (NAP1L3), mRNA [NM_004538] 3.0 NM_022166 XYLT1 Homo sapiens xylosyltransferase I (XYLT1), mRNA [NM_022166] 2.9 NM_207334 FAM43B Homo sapiens family with sequence similarity 43, member B (FAM43B), mRNA [NM_207334] 2.9 NM_019849 SLC7A10 Homo sapiens solute carrier family 7, (neutral amino acid transporter, y+ system) member 10 (SLC7A10), mRNA [NM_019849] 2.9 NM_024522 NKAIN1 Homo sapiens Na+/K+ transporting ATPase interacting 1 (NKAIN1), mRNA [NM_024522] 2.9 NM_001025266 C3orf70 Homo sapiens chromosome 3 open reading frame 70 (C3orf70), mRNA [NM_001025266] 2.9 NM_012190 ALDH1L1 Homo sapiens aldehyde dehydrogenase 1 family, member L1 (ALDH1L1), mRNA [NM_012190] 2.9 NM_032446 MEGF10 Homo sapiens multiple EGF-like-domains 10 (MEGF10), mRNA [NM_032446] 2.9 NM_001013398 IGFBP3 Homo sapiens insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 1, mRNA [NM_001013398] 2.9 NM_198270 NHS Homo sapiens Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant 1, mRNA [NM_198270] 2.8 NM_052862 RCSD1 Homo sapiens RCSD domain containing 1 (RCSD1), mRNA [NM_052862] 2.8 NM_053064 GNG2 Homo sapiens guanine nucleotide binding protein (G protein), gamma 2 (GNG2), mRNA [NM_053064] 2.8 Downloaded from AK126405 CMKLR1 Homo sapiens chemokine-like receptor 1 (CMKLR1), transcript variant 1, mRNA [NM_001142343] 2.8 NM_030775 WNT5B Homo sapiens wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2, mRNA [NM_030775] 2.8 NM_004750 CRLF1 Homo sapiens cytokine receptor-like factor 1 (CRLF1), mRNA [NM_004750] 2.8 NM_018271 THNSL2 Homo sapiens threonine synthase-like 2 (S. cerevisiae) (THNSL2), mRNA [NM_018271] 2.8 NM_004770 KCNB2 Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 2 (KCNB2), mRNA [NM_004770] 2.7

http://hyper.ahajournals.org/ NM_003933 BAIAP3 Homo sapiens BAI1-associated protein 3 (BAIAP3), mRNA [NM_003933] 2.7 NM_005070 SLC4A3 Homo sapiens solute carrier family 4, anion exchanger, member 3 (SLC4A3), transcript variant 1, mRNA [NM_005070] 2.7 NM_024572 GALNT14 Homo sapiens UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14) (GALNT14), mRNA [NM_024572] 2.7 NM_004861 GAL3ST1 Homo sapiens galactose-3-O-sulfotransferase 1 (GAL3ST1), mRNA [NM_004861] 2.7 NM_032932 RAB11FIP4 Homo sapiens RAB11 family interacting protein 4 (class II) (RAB11FIP4), mRNA [NM_032932] 2.7 AI754733 TGFBI AL573456 Homo sapiens PLACENTA COT 25-NORMALIZED Homo sapiens cDNA clone CS0DI051YC22 3-PRIME, mRNA sequence [AL573456] 2.7 AK055463 FLJ30901 Homo sapiens cDNA FLJ30901 fis, clone FEBRA2005778. [AK055463] 2.7 NM_016269 LEF1 Homo sapiens lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1, mRNA [NM_016269] 2.7 NM_014862 ARNT2 Homo sapiens aryl-hydrocarbon receptor nuclear translocator 2 (ARNT2), mRNA [NM_014862] 2.7 byguest onSeptember11, 2012 NM_019066 MAGEL2 Homo sapiens MAGE-like 2 (MAGEL2), mRNA [NM_019066] 2.7 NM_004329 BMPR1A Homo sapiens bone morphogenetic protein receptor, type IA (BMPR1A), mRNA [NM_004329] 2.6 NM_032621 BEX2 Homo sapiens brain expressed X-linked 2 (BEX2), mRNA [NM_032621] 2.6 NM_173484 KLF17 Homo sapiens Kruppel-like factor 17 (KLF17), mRNA [NM_173484] 2.6 NM_138325 PCSK6 Homo sapiens proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 6, mRNA [NM_138325] 2.6 BC020867 SLC6A13 Homo sapiens solute carrier family 6 (neurotransmitter transporter, GABA), member 13, mRNA (cDNA clone IMAGE:4594185), complete cds. [BC020867] 2.6 NM_024645 ZMAT4 Homo sapiens zinc finger, matrin type 4 (ZMAT4), transcript variant 1, mRNA [NM_024645] 2.6 NM_024025 DUSP26 Homo sapiens dual specificity phosphatase 26 (putative) (DUSP26), mRNA [NM_024025] 2.6 NM_004443 EPHB3 Homo sapiens EPH receptor B3 (EPHB3), mRNA [NM_004443] 2.6 NM_002606 PDE9A Homo sapiens phosphodiesterase 9A (PDE9A), transcript variant 1, mRNA [NM_002606] 2.6 NM_032208 ANTXR1 Homo sapiens anthrax toxin receptor 1 (ANTXR1), transcript variant 1, mRNA [NM_032208] 2.6 NM_000222 KIT Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 1, mRNA [NM_000222] 2.6 NM_032510 PARD6G Homo sapiens par-6 partitioning defective 6 homolog gamma (C. elegans) (PARD6G), mRNA [NM_032510] 2.6 NM_152495 CNIH3 Homo sapiens cornichon homolog 3 (Drosophila) (CNIH3), mRNA [NM_152495] 2.6 NM_194298 SLC16A9 Homo sapiens solute carrier family 16, member 9 (monocarboxylic acid transporter 9) (SLC16A9), mRNA [NM_194298] 2.6 NM_033543 CEACAM21 Homo sapiens carcinoembryonic antigen-related cell adhesion molecule 21 (CEACAM21), transcript variant 2, mRNA [NM_033543] 2.6 NM_021706 LAIR1 Homo sapiens leukocyte-associated immunoglobulin-like receptor 1 (LAIR1), transcript variant a, mRNA [NM_002287] 2.6 NM_001024660 KALRN Homo sapiens kalirin, RhoGEF kinase (KALRN), transcript variant 1, mRNA [NM_001024660] 2.6 NM_006486 FBLN1 Homo sapiens fibulin 1 (FBLN1), transcript variant D, mRNA [NM_006486] 2.6 NM_178456 C20orf85 Homo sapiens chromosome 20 open reading frame 85 (C20orf85), mRNA [NM_178456] 2.6 NM_001615 ACTG2 Homo sapiens actin, gamma 2, smooth muscle, enteric (ACTG2), mRNA [NM_001615] 2.6 NM_001007139 IGF2 Homo sapiens insulin-like growth factor 2 (somatomedin A) (IGF2), transcript variant 1, mRNA [NM_000612] 2.6 NM_000427 LOR Homo sapiens loricrin (LOR), mRNA [NM_000427] 2.6 NM_031890 CECR6 Homo sapiens cat eye syndrome chromosome region, candidate 6 (CECR6), mRNA [NM_031890] 2.5 NM_001257 CDH13 Homo sapiens cadherin 13, H-cadherin (heart) (CDH13), mRNA [NM_001257] 2.5 AK055279 UTP23 Homo sapiens cDNA FLJ30717 fis, clone FCBBF2001672. [AK055279] 2.5 Downloaded from AK074614 IGF2 Homo sapiens cDNA FLJ90133 fis, clone HEMBB1000567. [AK074614] 2.5 NM_018004 TMEM45A Homo sapiens transmembrane protein 45A (TMEM45A), mRNA [NM_018004] 2.5 NM_000216 KAL1 Homo sapiens Kallmann syndrome 1 sequence (KAL1), mRNA [NM_000216] 2.5 NM_153360 APCDD1L Homo sapiens adenomatosis polyposis coli down-regulated 1-like (APCDD1L), mRNA [NM_153360] 2.5 NM_001010925 ANKRD19 Homo sapiens cDNA FLJ36178 fis, clone TESTI2026534. [AK093497] 2.5

http://hyper.ahajournals.org/ NM_172364 CACNA2D4 Homo sapiens calcium channel, voltage-dependent, alpha 2/delta subunit 4 (CACNA2D4), mRNA [NM_172364] 2.5 NM_024603 BEND5 Homo sapiens BEN domain containing 5 (BEND5), mRNA [NM_024603] 2.5 NM_001845 COL4A1 Homo sapiens collagen, type IV, alpha 1 (COL4A1), mRNA [NM_001845] 2.5 NM_174953 ATP2A3 Homo sapiens ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 5, mRNA [NM_174953] 2.5 NM_001003395 TPD52L1 Homo sapiens tumor protein D52-like 1 (TPD52L1), transcript variant 2, mRNA [NM_001003395] 2.5 NM_080743 SRrp35 Homo sapiens serine-arginine repressor protein (35 kDa) (SRrp35), mRNA [NM_080743] 2.5 NM_001004439 ITGA11 Homo sapiens integrin, alpha 11 (ITGA11), mRNA [NM_001004439] 2.5 NM_032812 PLXDC2 Homo sapiens plexin domain containing 2 (PLXDC2), mRNA [NM_032812] 2.5 BC006530 BC006530 Homo sapiens hypothetical LOC554175, mRNA (cDNA clone MGC:11077 IMAGE:3688915), complete cds. [BC006530] 2.5 byguest onSeptember11, 2012 BC045778 XYLT1 Homo sapiens xylosyltransferase I (XYLT1), mRNA [NM_022166] 2.5 NM_152737 RNF182 Homo sapiens ring finger protein 182 (RNF182), mRNA [NM_152737] 2.5 BC011643 AUTS2 Homo sapiens autism susceptibility candidate 2 (AUTS2), transcript variant 3, mRNA [NM_001127232] 2.5 NM_000814 GABRB3 Homo sapiens gamma-aminobutyric acid (GABA) A receptor, beta 3 (GABRB3), transcript variant 1, mRNA [NM_000814] 2.4 NM_005378 MYCN Homo sapiens v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN), mRNA [NM_005378] 2.4 AK026826 ITGA9 Homo sapiens cDNA: FLJ23173 fis, clone LNG10019. [AK026826] 2.4 NM_015053 PPFIA4 Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (PPFIA4), mRNA [NM_015053] 2.4 NM_057157 CYP26A1 Homo sapiens cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1), transcript variant 2, mRNA [NM_057157] 2.4 NM_001482 GATM Homo sapiens glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein, mRNA [NM_001482] 2.4 NM_032342 C9orf125 Homo sapiens chromosome 9 open reading frame 125 (C9orf125), mRNA [NM_032342] 2.4 NM_017551 GRID1 Homo sapiens glutamate receptor, ionotropic, delta 1 (GRID1), mRNA [NM_017551] 2.4 NM_000775 CYP2J2 Homo sapiens cytochrome P450, family 2, subfamily J, polypeptide 2 (CYP2J2), mRNA [NM_000775] 2.4 NM_000855 GUCY1A2 Homo sapiens guanylate cyclase 1, soluble, alpha 2 (GUCY1A2), mRNA [NM_000855] 2.4 NM_001759 CCND2 Homo sapiens cyclin D2 (CCND2), mRNA [NM_001759] 2.4 NM_021785 RAI2 Homo sapiens retinoic acid induced 2 (RAI2), mRNA [NM_021785] 2.4 NM_001406 EFNB3 Homo sapiens ephrin-B3 (EFNB3), mRNA [NM_001406] 2.4 NM_004370 COL12A1 Homo sapiens collagen, type XII, alpha 1 (COL12A1), transcript variant long, mRNA [NM_004370] 2.4 NM_018962 DSCR6 Homo sapiens Down syndrome critical region gene 6 (DSCR6), mRNA [NM_018962] 2.4 AK094413 C9orf125 Transmembrane protein C9orf125 [Source:UniProtKB/Swiss-Prot;Acc:Q9BRR3] [ENST00000374851] 2.4 NM_006113 VAV3 Homo sapiens vav 3 guanine nucleotide exchange factor (VAV3), transcript variant 1, mRNA [NM_006113] 2.4 AK097804 C20orf112 Homo sapiens cDNA FLJ40485 fis, clone TESTI2043857, moderately similar to Homo sapiens nolp mRNA. [AK097804] 2.4 NM_003671 CDC14B Homo sapiens CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 2, mRNA [NM_033331] 2.4 NM_145056 DACT3 Homo sapiens dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis) (DACT3), mRNA [NM_145056] 2.4 NM_198594 C1QTNF1 Homo sapiens C1q and tumor necrosis factor related protein 1 (C1QTNF1), mRNA [NM_198594] 2.4 NM_004072 CMKLR1 Homo sapiens chemokine-like receptor 1 (CMKLR1), transcript variant 2, mRNA [NM_004072] 2.3 NM_013363 PCOLCE2 Homo sapiens procollagen C-endopeptidase enhancer 2 (PCOLCE2), mRNA [NM_013363] 2.3 Downloaded from NM_019854 PRMT8 Homo sapiens protein arginine methyltransferase 8 (PRMT8), mRNA [NM_019854] 2.3 NM_003105 SORL1 Homo sapiens sortilin-related receptor, L(DLR class) A repeats-containing (SORL1), mRNA [NM_003105] 2.3 NM_001100 ACTA1 Homo sapiens actin, alpha 1, skeletal muscle (ACTA1), mRNA [NM_001100] 2.3 NM_001558 IL10RA Homo sapiens interleukin 10 receptor, alpha (IL10RA), mRNA [NM_001558] 2.3 NM_004321 KIF1A Homo sapiens kinesin family member 1A (KIF1A), mRNA [NM_004321] 2.3

http://hyper.ahajournals.org/ NM_003918 GYG2 Homo sapiens glycogenin 2 (GYG2), transcript variant 2, mRNA [NM_003918] 2.3 NM_019590 KIAA1217 Homo sapiens KIAA1217 (KIAA1217), transcript variant 1, mRNA [NM_019590] 2.3 NM_002529 NTRK1 Homo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2, mRNA [NM_002529] 2.3 NM_003458 BSN Homo sapiens bassoon (presynaptic cytomatrix protein) (BSN), mRNA [NM_003458] 2.3 NM_022071 SH2D4A Homo sapiens SH2 domain containing 4A (SH2D4A), mRNA [NM_022071] 2.3 NM_014548 TMOD2 Homo sapiens tropomodulin 2 (neuronal) (TMOD2), transcript variant 1, mRNA [NM_014548] 2.3 NM_021136 RTN1 Homo sapiens reticulon 1 (RTN1), transcript variant 1, mRNA [NM_021136] 2.3 NM_000399 EGR2 Homo sapiens early growth response 2 (Krox-20 homolog, Drosophila) (EGR2), transcript variant 1, mRNA [NM_000399] 2.3 NM_023943 TMEM108 Homo sapiens transmembrane protein 108 (TMEM108), transcript variant 1, mRNA [NM_023943] 2.3 byguest onSeptember11, 2012 NM_023002 HAPLN4 Homo sapiens hyaluronan and proteoglycan link protein 4 (HAPLN4), mRNA [NM_023002] 2.3 NM_198469 MORN5 Homo sapiens MORN repeat containing 5 (MORN5), mRNA [NM_198469] 2.3 NM_000439 PCSK1 Homo sapiens proprotein convertase subtilisin/kexin type 1 (PCSK1), mRNA [NM_000439] 2.3 NM_006690 MMP24 Homo sapiens matrix metallopeptidase 24 (membrane-inserted) (MMP24), mRNA [NM_006690] 2.3 NM_032387 WNK4 Homo sapiens WNK lysine deficient protein kinase 4 (WNK4), mRNA [NM_032387] 2.3 NM_173549 C8orf47 Homo sapiens chromosome 8 open reading frame 47 (C8orf47), mRNA [NM_173549] 2.3 NM_004439 EPHA5 Homo sapiens EPH receptor A5 (EPHA5), transcript variant 1, mRNA [NM_004439] 2.3 NM_006017 PROM1 Homo sapiens prominin 1 (PROM1), mRNA [NM_006017] 2.3 NM_005822 RCAN2 Homo sapiens regulator of calcineurin 2 (RCAN2), mRNA [NM_005822] 2.3 NM_001843 CNTN1 Homo sapiens contactin 1 (CNTN1), transcript variant 1, mRNA [NM_001843] 2.3 NM_133625 SYN2 Homo sapiens synapsin II (SYN2), transcript variant IIa, mRNA [NM_133625] 2.2 NM_030915 LBH Homo sapiens limb bud and heart development homolog (mouse) (LBH), mRNA [NM_030915] 2.2 NM_014020 TMEM176B Homo sapiens transmembrane protein 176B (TMEM176B), transcript variant 1, mRNA [NM_014020] 2.2 NM_003638 ITGA8 Homo sapiens integrin, alpha 8 (ITGA8), mRNA [NM_003638] 2.2 NM_005883 APC2 Homo sapiens adenomatosis polyposis coli 2 (APC2), mRNA [NM_005883] 2.2 NM_003474 ADAM12 Homo sapiens ADAM metallopeptidase domain 12 (ADAM12), transcript variant 1, mRNA [NM_003474] 2.2 NM_002247 KCNMA1 Homo sapiens potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 2, mRNA [NM_002247] 2.2 NM_000681 ADRA2A Homo sapiens adrenergic, alpha-2A-, receptor (ADRA2A), mRNA [NM_000681] 2.2 NM_017412 FZD3 Homo sapiens frizzled homolog 3 (Drosophila) (FZD3), mRNA [NM_017412] 2.2 NM_004441 EPHB1 Homo sapiens EPH receptor B1 (EPHB1), mRNA [NM_004441] 2.2 NM_022055 KCNK12 Homo sapiens potassium channel, subfamily K, member 12 (KCNK12), mRNA [NM_022055] 2.2 NM_018651 ZNF167 Homo sapiens zinc finger protein 167 (ZNF167), transcript variant 1, mRNA [NM_018651] 2.2 NM_001819 CHGB Homo sapiens chromogranin B (secretogranin 1) (CHGB), mRNA [NM_001819] 2.2 NM_178860 SEZ6 Homo sapiens seizure related 6 homolog (mouse) (SEZ6), transcript variant 1, mRNA [NM_178860] 2.2 NM_016148 SHANK1 Homo sapiens SH3 and multiple ankyrin repeat domains 1 (SHANK1), mRNA [NM_016148] 2.2 NM_182908 DHRS2 Homo sapiens dehydrogenase/reductase (SDR family) member 2 (DHRS2), transcript variant 1, mRNA [NM_182908] 2.2 Downloaded from AF230201 TSHZ2 Teashirt homolog 2 (Zinc finger protein 218)(Ovarian cancer-related protein 10-2)(OVC10-2) [Source:UniProtKB/Swiss-Prot;Acc:Q9NRE2] [ENST00000371497 2.2 NM_138576 BCL11B Homo sapiens B-cell CLL/lymphoma 11B (zinc finger protein) (BCL11B), transcript variant 1, mRNA [NM_138576] 2.2 NM_033181 CNR1 Homo sapiens cannabinoid receptor 1 (brain) (CNR1), transcript variant 2, mRNA [NM_033181] 2.2 NM_170735 BDNF Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 1, mRNA [NM_170735] 2.2 NM_025248 SNIP Homo sapiens SNAP25-interacting protein (SNIP), mRNA [NM_025248] 2.2

http://hyper.ahajournals.org/ NM_021614 KCNN2 Homo sapiens potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 (KCNN2), transcript variant 1, mRNA [NM_02161 2.2 NM_053034 ANTXR1 Homo sapiens anthrax toxin receptor 1 (ANTXR1), transcript variant 2, mRNA [NM_053034] 2.2 NM_178140 PDZD2 Homo sapiens PDZ domain containing 2 (PDZD2), mRNA [NM_178140] 2.2 NM_018420 SLC22A15 Homo sapiens solute carrier family 22, member 15 (SLC22A15), mRNA [NM_018420] 2.2 NM_021641 ADAM12 Homo sapiens ADAM metallopeptidase domain 12 (ADAM12), transcript variant 2, mRNA [NM_021641] 2.2 NM_001039570 KREMEN1 Homo sapiens kringle containing transmembrane protein 1 (KREMEN1), transcript variant 3, mRNA [NM_001039570] 2.2 AK124788 GGT7 Homo sapiens cDNA FLJ42798 fis, clone BRAWH3008931. [AK124788] 2.2 NM_005739 RASGRP1 Homo sapiens RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1, mRNA [NM_005739] 2.2 NM_020796 SEMA6A Homo sapiens sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A), mRNA [NM_020796] 2.2 byguest onSeptember11, 2012 AB023211 PADI2 Homo sapiens peptidyl arginine deiminase, type II (PADI2), mRNA [NM_007365] 2.2 NM_000679 ADRA1B Homo sapiens adrenergic, alpha-1B-, receptor (ADRA1B), mRNA [NM_000679] 2.2 NM_173798 ZCCHC12 Homo sapiens zinc finger, CCHC domain containing 12 (ZCCHC12), mRNA [NM_173798] 2.2 NM_152309 PIK3AP1 Homo sapiens phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), mRNA [NM_152309] 2.1 NM_003155 STC1 Homo sapiens stanniocalcin 1 (STC1), mRNA [NM_003155] 2.1 AK025887 SLC2A9 Homo sapiens cDNA: FLJ22234 fis, clone HRC02022. [AK025887] 2.1 NM_033331 CDC14B Homo sapiens CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 2, mRNA [NM_033331] 2.1 NM_014951 ZNF365 Homo sapiens zinc finger protein 365 (ZNF365), transcript variant A, mRNA [NM_014951] 2.1 BX110856 SLC8A2 Homo sapiens solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2), mRNA [NM_015063] 2.1 NM_019065 NECAB2 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), mRNA [NM_019065] 2.1 NM_052934 SLC26A9 Homo sapiens solute carrier family 26, member 9 (SLC26A9), transcript variant 1, mRNA [NM_052934] 2.1 NM_018245 OGDHL Homo sapiens oxoglutarate dehydrogenase-like (OGDHL), mRNA [NM_018245] 2.1 NM_001849 COL6A2 Homo sapiens collagen, type VI, alpha 2 (COL6A2), transcript variant 2C2, mRNA [NM_001849] 2.1 NM_153456 HS6ST3 Homo sapiens heparan sulfate 6-O-sulfotransferase 3 (HS6ST3), mRNA [NM_153456] 2.1 NM_152322 BTBD11 Homo sapiens BTB (POZ) domain containing 11 (BTBD11), transcript variant a, mRNA [NM_001018072] 2.1 NM_020066 FMN2 Homo sapiens formin 2 (FMN2), mRNA [NM_020066] 2.1 NM_019644 ANKRD7 Homo sapiens ankyrin repeat domain 7 (ANKRD7), transcript variant 1, mRNA [NM_001077708] 2.1 NM_017563 IL17RD Homo sapiens interleukin 17 receptor D (IL17RD), mRNA [NM_017563] 2.1 NM_032040 CCDC8 Homo sapiens coiled-coil domain containing 8 (CCDC8), mRNA [NM_032040] 2.1 NM_173160 FXYD4 Homo sapiens FXYD domain containing ion transport regulator 4 (FXYD4), mRNA [NM_173160] 2.1 NM_003408 ZFP37 Homo sapiens zinc finger protein 37 homolog (mouse) (ZFP37), mRNA [NM_003408] 2.1 NM_006072 CCL26 Homo sapiens chemokine (C-C motif) ligand 26 (CCL26), mRNA [NM_006072] 2.1 NM_017899 TESC Homo sapiens tescalcin (TESC), mRNA [NM_017899] 2.1 NM_014817 KIAA0644 Homo sapiens KIAA0644 gene product (KIAA0644), mRNA [NM_014817] 2.1 AK054929 NCAM1 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 3, mRNA [NM_001076682] 2.1 NM_019609 CPXM1 Homo sapiens carboxypeptidase X (M14 family), member 1 (CPXM1), mRNA [NM_019609] 2.1 Downloaded from NM_153183 NUDT10 Homo sapiens nudix (nucleoside diphosphate linked moiety X)-type motif 10 (NUDT10), mRNA [NM_153183] 2.1 NM_139055 ADAMTS15 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15), mRNA [NM_139055] 2.1 NM_201575 SEZ6L2 Homo sapiens seizure related 6 homolog (mouse)-like 2 (SEZ6L2), transcript variant 2, mRNA [NM_201575] 2.1 NM_173666 DTWD2 Homo sapiens DTW domain containing 2 (DTWD2), mRNA [NM_173666] 2.1 NM_014571 HEYL Homo sapiens hairy/enhancer-of-split related with YRPW motif-like (HEYL), mRNA [NM_014571] 2.1

http://hyper.ahajournals.org/ NM_005360 MAF Homo sapiens v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) (MAF), transcript variant 2, mRNA [NM_001031804] 2.0 NM_018659 CYTL1 Homo sapiens cytokine-like 1 (CYTL1), mRNA [NM_018659] 2.0 NM_014861 ATP2C2 Homo sapiens ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2), mRNA [NM_014861] 2.0 NM_031302 GLT8D2 Homo sapiens glycosyltransferase 8 domain containing 2 (GLT8D2), mRNA [NM_031302] 2.0 NM_005314 GRPR Homo sapiens gastrin-releasing peptide receptor (GRPR), mRNA [NM_005314] 2.0 NM_020225 STOX2 Homo sapiens storkhead box 2 (STOX2), mRNA [NM_020225] 2.0 NM_018354 C20orf46 Homo sapiens chromosome 20 open reading frame 46 (C20orf46), mRNA [NM_018354] 2.0 NM_182511 CBLN2 Homo sapiens cerebellin 2 precursor (CBLN2), mRNA [NM_182511] 2.0 NM_024781 CCDC102B Homo sapiens coiled-coil domain containing 102B (CCDC102B), transcript variant 2, mRNA [NM_024781] 2.0 byguest onSeptember11, 2012 NM_005825 RASGRP2 Homo sapiens RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 2, mRNA [NM_153819] 2.0 AK125591 IL17RD Homo sapiens interleukin 17 receptor D (IL17RD), mRNA [NM_017563] 2.0 NM_001542 IGSF3 Homo sapiens immunoglobulin superfamily, member 3 (IGSF3), transcript variant 1, mRNA [NM_001542] 2.0 NM_003728 UNC5C Homo sapiens unc-5 homolog C (C. elegans) (UNC5C), mRNA [NM_003728] 2.0 NM_000683 ADRA2C Homo sapiens adrenergic, alpha-2C-, receptor (ADRA2C), mRNA [NM_000683] 2.0 NM_153229 TMEM92 Homo sapiens transmembrane protein 92 (TMEM92), mRNA [NM_153229] 2.0 NM_006108 SPON1 Homo sapiens spondin 1, extracellular matrix protein (SPON1), mRNA [NM_006108] 2.0 NM_024761 MOBKL2B Homo sapiens MOB1, Mps One Binder kinase activator-like 2B (yeast) (MOBKL2B), mRNA [NM_024761] 2.0 NM_002236 KCNF1 Homo sapiens potassium voltage-gated channel, subfamily F, member 1 (KCNF1), mRNA [NM_002236] 2.0 NM_198721 COL25A1 Homo sapiens collagen, type XXV, alpha 1 (COL25A1), transcript variant 1, mRNA [NM_198721] 2.0 AK056190 DFNB31 Homo sapiens cDNA FLJ31628 fis, clone NT2RI2003344. [AK056190] 2.0 NM_000576 IL1B Homo sapiens interleukin 1, beta (IL1B), mRNA [NM_000576] 2.0 BC036669 COL25A1 Homo sapiens collagen, type XXV, alpha 1, mRNA (cDNA clone MGC:33431 IMAGE:5268875), complete cds. [BC036669] 2.0 NM_015692 CPAMD8 Homo sapiens C3 and PZP-like, alpha-2-macroglobulin domain containing 8 (CPAMD8), mRNA [NM_015692] 2.0 AY335940 IRX2 Homo sapiens iroquois homeobox 2 (IRX2), transcript variant 1, mRNA [NM_033267] 2.0 NM_145173 DIRAS1 Homo sapiens DIRAS family, GTP-binding RAS-like 1 (DIRAS1), mRNA [NM_145173] 2.0 NM_000250 MPO Homo sapiens myeloperoxidase (MPO), nuclear gene encoding mitochondrial protein, mRNA [NM_000250] 2.0 Table S5: Genes associated with pathways that are significantly overrepresented among Ezh2 target genes (PANTHER analysis)

Alzheimer inflammation mediated endogenous Wnt cadherin integrin alpha-adrenergic pathway disease- angiogenesis by chemokine cell cycle cannabinoid signaling signaling signaling receptor signaling presenilin and cytokine signaling signaling number of genes on homo sapiens 317 122 147 181 191 283 32 22 24 Downloaded from reference list p -value 0.00002 0.00004 0.0002 0.0007 0.001 0.001 0.009 0.033 0.039

target genes ACTA1 ACTA1 ACTA1 ACTA1 APC2 ACTA1 ADRA1B APC2 CNR1 ACTG2 ACTG2 ACTG2 ACTG2 EPHA5 ACTG2 ADRA2A CCND2 GNG2 http://hyper.ahajournals.org/ APC2 FZD3 CDH13 COL12A1 EPHB1 CCL26 ADRA2C BMPR1A FZD7 CNR1 COL4A1 EPHB3 COL12A1 CDH13 LEF1 FZD3 COL6A2 FGF1 COL21A1 CNR1 MMP24 FZD7 ITGA11 FZD3 COL6A2 DACT1 PCSK1 LEF1 ITGA4 NOS2 GNG2 FZD3 PCSK6 PCDHB2 ITGA8 SH2D4A IL1B FZD7 WNT5B WNT5B ITGA9 WNT5B ITGA4 GNG2 ITGA9

byguest onSeptember11, 2012 KREMEN1 VAV3 LEF1 MYCN PCDHB2 WNT5B Figure S1

A 1.2 n = 9 1.0

0.8

0.6 * 0.4

fold expression 0.2 [arbitrary units ± SEM] units [arbitrary 0.0 control siRNA Ezh2 siRNA

B siRNA control Ezh2

Ezh2

-actin

1.0 n = 4

0.5 * 0.0 fold expression fold control Ezh2 siRNA

Knock down of Ezh2 72 hours after siRNA transfection on the mRNA (A) and protein (B) level. The diagram depicts densitometric analysis of four independent experiments (normalized to control-siRNA and to the -actin loading control). *) p < 0.05 vs. control.

Protein expression of Ezh2 (#3147, Cell Signaling, Danvers, MA) was analyzed by immunoblotting as recently described [Cardiovasc Res. 2010 Jan 15;85(2):395-403].

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Figure S2

A 7 p = 0.168 6 n = 3 5

4

3 cell countcell cells/mL ±SD] 5 2

[10 1

0 control Ezh2

B 120 p = 0.726 n = 3 100

80

60

40

viable cells [% cells ± SD] viable 20

0 control Ezh2

Cell proliferation (A) and viability (B) after Ezh2 knock down.

Cells were stained using 0.1% Erythrosin B (AppliChem) and counted 72 hours after transfection with siRNA directed against Ezh2.

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 cell adhesion cell communication (GO:0007155) (GO:0007154) gene ID fold expression gene ID fold expression Figure S3 CD24 8.4 FGF13 15.6 5fold FAT3 6.4 CD24 8.4 ITGA4 5.8 IGFBP5 6.6 FGF1 5.6 FGF1 5.6 PCDH17 5.2 PCDHB2 5.1 PCDHB2 5.1 C1orf187 4.6 Heat maps of genes whose expression VCAN 4.3 CXXC4 4.3 PCDHB10 4.2 PCDHB10 4.2 changed by more than twofold after PCDHB5 4.1 PCDHB5 4.1 SPON2 4.0 HEY2 4.0 knock down of Ezh2 (n = 3) and that PCDHB9 3.7 NOS2 3.9 COL3A1 3.5 PCDHB9 3.7 are associated with the GO terms ‘cell NCAM2 3.4 NPTX2 3.6 MUC4 3.3 SNAI2 3.5 adhesion’ or ‘cell communication’. FZD7 3.2 IRS1 3.4 PCDHB16 3.1 TGFA 3.4 EPDR1 3.1 GJC3 3.3 TGFB2 3.1 FZD7 3.2 COL21A1 3.0 IL11 3.1 PCDH15 2.9 PCDHB16 3.1 PCDHB14 2.9 TGFB2 3.1 -5fold FLRT3 2.9 RASGRF2 3.0 AOC3 2.9 PCDHB14 2.9 FREM3 2.9 FREM3 2.9 MEGF10 2.9 CARD9 2.9 POSTN 2.8 IGFBP3 2.9 PCDHB15 2.8 WNT5B 2.8 SERPINI1 2.8 BAIAP3 2.7 CDH10 2.7 GAL3ST1 2.7 TGFBI 2.7 PTGS2 2.7 LEF1 2.7 BMPR1A 2.6 ANTXR1 2.6 KIT 2.6 CDH13 2.5 KALRN 2.6 KAL1 2.5 IGF2 2.6 ITGA11 2.5 CDH13 2.5 ITGA9 2.4 IGF1 2.5 PCDHB12 2.4 MAP2K6 2.5 FREM1 2.4 TPD52L1 2.5 COL12A1 2.4 KCNMB4 2.5 CDH15 2.4 GABRB3 2.4 VAV3 2.4 SYT1 2.4 HAPLN4 2.3 PPFIA4 2.4 CNTN1 2.3 CYP26A1 2.4 ITGA8 2.2 FREM1 2.4 ADAM12 2.2 EFNB3 2.4 BMPR1B 2.2 CDKN2B 2.4 PCDHB11 2.2 VAV3 2.4 LAMA2 2.2 BSN 2.3 PDZD2 2.2 TMOD2 2.3 ADIPOQ 2.1 EGR2 2.3 APBA1 2.1 RASGRP3 2.3 BCL2 2.1 PCSK1 2.3 PCDHA1 2.1 SYN2 2.2 COL6A2 2.1 ITGA8 2.2 TESC 2.1 KCNMA1 2.2 NCAM1 2.1 ADRA2A 2.2 EDIL3 2.1 EPHB1 2.2 CPXM1 2.1 PCDHB11 2.2 SPON1 2.0 CNR1 2.2 IL1B 2.0 BDNF 2.2 C4orf31 -2.0 SH3PXD2A 2.2 ITGAD -2.0 KREMEN1 2.2 TGM2 -2.0 RASGRP1 2.2 SELP -2.0 ADRA1B 2.2 SCARB1 -2.1 ADIPOQ 2.1 SELE -2.1 APBA1 2.1 CLDN11 -2.1 STC1 2.1 IL18 -2.2 SLC8A2 2.1 CLDN17 -2.2 CCL26 2.1 COL4A6 -2.2 NCAM1 2.1 LAMA3 -2.2 TSPAN32 2.0 BTBD9 -2.2 RASGRP2 2.0 DDR1 -2.2 ADRA2C 2.0 KIRREL2 -2.2 IL1B 2.0 CRNN -2.3 IL1RL1 -2.0 BOC -2.3 TGM2 -2.0 CCL2 -2.3 STAR -2.0 ICAM1 -2.5 CLDN11 -2.1 PPFIA2 -2.6 NQO1 -2.1 CD22 -2.7 GUCA1B -2.1 CDH6 -3.0 C9orf100 -2.1 VCAM1 -3.0 CACNB2 -2.1 CDH9 -3.8 PLEKHG4B -2.2 IL18 -2.2 TNFSF18 -2.2 UTS2 -2.2 HRK -2.2 SECTM1 -2.2 APOL3 -2.2 FGF16 -2.3 CCL2 -2.3 LGALS9 -2.3 EZH2 -2.4 HTR1F -2.4 TSHR -2.5 BIRC7 -2.5 IL26 -2.7 TLR3 -2.7 CASP1 -2.8 CPLX3 -2.8 CD74 -2.8 CYP26B1 -2.9 ARHGEF4 -3.0 CCL20 -3.3 MME -3.5 UBD -4.6 Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012 Figure S4

GO:0008150 biological process

GO:0022610 GO:0009987 biological adhesion cellular process

GO:0007155 GO:0007154 cell adhesion cell communication

Parent child relationships of significantly overrepresented GO terms of genes that are regulated more than twofold 72 hours after knock down of Ezh2 (p < 0.01, n = 3). Color intensity increases with statistical significance.

Downloaded from http://hyper.ahajournals.org/ by guest on September 11, 2012