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(878) Aims: 1. To enable candidates to acquire the knowledge 4. To create awareness about the appreciation of and develop an understanding of how materials biological processes to industries. are provided by biological agents to provide 5. To develop the ability to appreciate biological goods and services. phenomenon in nature and the contribution of 2. To appreciate the role played by biotechnology biotechnology to human welfare. in improving health care for human beings. 6. To develop scientific attitude towards 3. To understand the interdisciplinary nature of biological phenomenon. this subject. CLASS XI There will be two papers in the subject: Application of these technologies for large- scale production, with special reference to Paper I: Theory…………... 3 hours ... 70 marks fermentation (Beer production only). Quality Paper II: Practical………. 3 hours ... 15 marks control management of the products, good Project Work………. …10 marks practices. Practical File…………. …5 marks (ii) Scope and importance of biotechnology: different branches of biotechnology and PAPER I –THEORY- 70 Marks different regulatory guidelines; ethical, legal and social issues (ELSI) that a There will be one paper of three hours duration biotechnologist comes across while doing the divided into two parts. work. Various organisations in the field of Part 1 (20 marks) will consist of compulsory short biotechnology. answer questions, testing knowledge, application and Names, definitions and importance of various skills relating to elementary/fundamental aspects of fields that can be covered under the entire syllabus. biotechnology such as - agricultural/ plant Part 2 (50 marks) will consist of eight questions out biotechnology, animal biotechnology/medical of which the candidates will be required to answer biotechnology, nanobiotechnology, industrial five questions. Each question in this part shall carry biotechnology, immunology and health care, 10 marks. energy and environment. 1. Introduction to Biotechnology Intellectual Property Rights (IPRs) in biotechnology- concept of intellectual (i) Historical background; definition; a brief property, types of IPR and its need; introduction of the traditional and modern intellectual property rights and the choice of techniques of Biotechnology and their intellectual property rights protection. applications. Discovery and invention; Concept of Definition of biotechnology by OECD and patenting, trademark, trade secrets, EFB; contributions of Karl Ereky and Louis copyright, geographical indications and Pasteur; use of various fermented products PBRs and their need. in ancient civilisations; Concept of ethical, legal and social issues Kitchen (traditional), the first with one common example IVF. biotechnological laboratory -reasoning Biosafety issues: release of genetically behind the technology involved in simple modified organisms into the environment and biological products like curd and beer; their impact; GEAC and its objectives. names of involved in their production. 350 Biotechnology - global and Indian scenario. Concept of chromosomal number in different Various institutes, centres and funding species, e.g. man, mouse, Drosophila and agencies - NBTB, CCMB, ICGEB, ICMR, pea. ICAR, DBT, DST which deal with Techniques in cytology – (light biotechnology and bioinformatics in India: and electron ), karyotyping and names only. centrifugation (principle and applications (iii) Basic concepts of Biochemical technology only). and biostatistics: What does the biochemical (ii) Cell Division and cell cycle: types of cell technology mean? An understanding of divisions and various other activities of cell various statistical methods involved in such as biochemical transformations. biotechnology. Types and significance of cell division and a Concept of buffer, type and preparation of brief note about the different stages of cell buffers, pH, physical variables; division – mitosis and meiosis. fermentation; An understanding of bio- reactors, idea of sampling – quadrat and Basic concept of cell cycle and cell cycle transect; measures of central tendency – regulation – CdK method only, definition of mean, median, mode; standard deviation and Mitotic Index. standard error; concept of probability – Biochemical Transformations: theoretical and experimental. An understanding of biochemical 2. transformations, different biochemical (i) Cell: Justification of cell as a basic unit of pathways involved in respiration - aerobic life. Prokaryotic cell and eukaryotic cell; A and anaerobic. brief note on the cell components with Aerobic respiration - Glycolysis, Krebs’ special reference to nucleus. Various cycle, electron transport chain and oxidative cytological techniques used in identifying the phosphorylation. cell and chromosomes. Anaerobic respiration - lactic acid, Differentiation prokaryotic and eukaryotic fermentation and alcohol fermentation – cellular systems. definition only. Structure of (in brief, with reference (iii) Errors in cell division: what happens if the to plasmid). Gram+ and Gram- bacteria. cell does not divide normally? An An understanding of cell components, their understanding of different numerical and basic structure and functions - cell wall, cell structural abnormalities. membrane, cytoplasmic reticulum, Golgi Concept of mutation: causes; types –somatic, apparatus, mitochondria, ribosomes, germinal, spontaneous, induced, gene, vacuoles, plastids, lysosomes, nucleus and chromosomal and genomatic mutations, other important inclusions of the cell. euploidy, aneuploidy, monosomy, nullisomy, Chromosomal structure and composition – trisomy and tetrasomy; various factors organisation of chromatids, concept of causing mutations. homologous and non-homologous Concept of non-disjunction: meiotic chromosomes, sister and non-sister non-disjunction and mitotic non-disjunction. chromatids, classification of chromosomes on Non-disjunction in sex chromosomes – the basis of position of the centromere on the Turner’s syndrome and Klinefelter’s chromosome, basic idea about telomere, syndrome - chromosomal composition and chromatin and nucleosome. An idea about symptoms only. banding patterns (Q, R, C and G) and their application.

351 Numerical chromosomal aberrations with energy; mechanism of enzyme action - lock respect to autosomes, i.e. Down’s syndrome and key model; induced fit hypothesis; factors –chromosomal composition and symptoms affecting enzyme activity (temperature, pH, only. substrate concentration, enzyme concentration, inhibitors (competitive, non- Structural chromosomal abnormalities – competitive). deletions, duplications, translocations, inversions. Optical activity of biomolecules (dextrorotatory and laevorotatory). Polyploidy and its significance in plants. Concept of supramolecular assembly. Inborn errors of metabolism - basic concept and examples like albinism, sickle cell (ii) Techniques used for separation of anaemia, phenylketonuria and alkaptonuria. biomolecules Ion exchange chromatography and paper 3. Biomolecules and related techniques chromatography. (i) Introduction to biomolecules- definition and types. Carbohydrates, proteins, lipids, 4. Developmental Biology and Immunology vitamins and enzymes – their structure and (i) Animal and plant development: development properties. of an organism from zygotic cell in both Biomolecules – definition and types plants and animals. Structure and functions of carbohydrates. Animal development – fertilisation, zygote to blastocyst formation. Sugars and derivatives; classification of some important mono, di and polysaccharides - Plant development. Double fertilisation glucose, fructose, glycogen, cellulose, chitin including formation of primary endosperm and peptidoglycan. Physical and chemical nucleus. properties of sugars. (ii) An understanding of defence strategies in Structure, functions and classification of living organisms. proteins i.e. simple, complex and derived; Immune system in higher animals, concept of building blocks of proteins - the amino acids: immunity, immunisation, antigen and chemical structure, types (acidic, basic and antibody. Various cells involved in immune neutral); physical and chemical properties of response in humans. An introduction to amino acids. 3D - structure of proteins. human leukocyte antigens with reference to Different types of protein structures - primary, organ transplantation; Types of immunity - secondary (alpha helix, beta pleated sheet and innate and acquired. ELISA Technique random structures), tertiary, quaternary; (Enzyme Linked Immuno Sorbent Assay). protein sequencing by MALDI-MS. Secondary metabolites in plants and their Structure and functions of lipids – fatty acids significance and alcohol; types (simple, conjugated and derived lipids with one example of each); Defence strategies in bacteria – endospores chemical and physical properties of lipids. and R plasmids. Vitamins: Definition, types (fat soluble and 5. Genetics water soluble vitamins); co-enzyme forms of water soluble vitamins; deficiency diseases of (i) Laws of Inheritance: An account of vitamins. Mendel’s experiments. Different types of genetic inheritance. Enzymes: Structure and functions of enzymes: chemical nature of enzymes; characteristics Mendel’s experiment on pea plant and his and properties of enzymes. An understanding laws of inheritance. of enzyme activity on the basis of activation

352 Concept of trait, gene, allele, phenotype, only once and destroy it. (d) Do not prick or use genotype, homozygosity, heterozygosity and blood drop in an indiscriminatory way. hemizygosity. Types of inheritance: 2. Identification of different types of blood cells by autosomal inheritance - dominant, co- preparing blood smear using Leishmann’s stain. dominant, recessive, polygenic, pleiotropic and cytoplasmic inheritance (plastidial Requirements: Blood sample, disposable needles, inheritance). slides, Leishmann’s stain. Make a blood smear on a slide, use the stain to colour the smear, Pedigree construction using different wash and observe under microscope. standard symbols. Sex chromosome inheritance - with special 3. Instruments – their names, use and principles (if reference to X chromosomal inheritance applicable). with suitable examples (colour blindness Water bath, pH meter, weighing balance, and haemophilia). desiccators, microfiltration unit, magnetic (ii) Gene Mapping: mapping of genes on stirrer, LAF, haemocytometer, micropipette, chromosomes using linkage analysis. , /spectrophotometer, hot Cancer and its genetics. air oven, , , electrophoresis chamber, colony counter, autoclave, . Mapping of genes on chromosomes with respect to COV (Crossing Over Value). 4. Finding out the pH of water by using pH meter or Basic concept of linkage (types not pH paper on tap water and water containing acid, required) and crossing over. Genetic base. recombination. Take tap water in three test tubes, add two drops Cancer: Causes (physical, chemical, of dil. HCl in one, two drops of NaOH in the biological – TSG and oncogenes); diagnosis second while leaving the third with tap and treatment. water. Use pH meter or pH paper to find their specific pH. (iii) Genes in populations: how do genes behave in populations from generation to 5. Observation of steps of mitosis by using the root generation? Various ways of studying tip of onion. population genetics. The students should be given practice in preparing slides for study of mitosis by crush Concept of gene pool and allele frequency, smear method. They should be able to identify definition of Hardy Weinberg law, its different stages (at least four stages). The applications. requirement for this set of experiments is Possibility of disease resistant and Acetocarmine stain slides, coverslips, susceptible genes in population. Definition and spirit-lamp. and application of pharmacogenetics and pharmacogenomics. 6. Measurement of mitotic index. Mitotic index is the ratio of number of cells PAPER II undergoing mitosis to the number of cells in the field. PRACTICAL WORK – 15 Marks No. of cells showing mitosis Candidates are required to complete the following MI = experiments. Total no. of cells in the field

1. Determination of blood group by using antisera. 7. Observation of various stages of meiosis under The students can perform this experiment on microscope. their own blood groups. Proper instructions For the study of meiosis, the students should be however are to be given for ‘prick’ – e.g. (a) shown permanent slides of meiosis and they Sterilize finger with alcohol/disinfectant. (b) Use should be able to identify at least six stages of only disposable sterile needle. (c) Use the needle meiosis from the slides.

353 8. Effect of temperature on curdling of milk by 10. Finding out the purity of milk by using using Lactobacillus bacteria at 37oC, 60oC and lactometer. o 10 C. Put the instrument in milk. If it sinks down Optimum temperature for curdling of milk is and reaches the mark ‘M’ mentioned on 37oC due to active form of bacteria at this lactometer, it means that the milk is pure or temperature; it is inactive at low temperature if not, it means that the milk is impure. If the and dies at high temperature. milk is mixed with water, it would sink higher than mark ‘M’. If it stands at the mark 3 it 9. Food tests: means that the milk is 75% pure and (i) Carbohydrates – starch by iodine solution respectively 2 for 50% purity and 1 for 25% turning blue - black in colour. purity. Reducing and non-reducing sugars by using 11. Construction of pedigree showing different Fehling’s solution / Benedict’s solution – types of inheritance. reducing the cupric ion (blue) to cuprous ion The students are to observe the traits like, (red). rolling of the tongue/ attached earlobe/ widow’s peak. (ii) Protein test – Biuret test, Xanthoproteic and Millon’s test 12. Preparation of karyotypes. Demonstration of any metaphasic plate of (a) For Biuret test –The protein produces mitosis. deep blue – violet colour due to the involvement of cupric ion in the product 13. Sampling methods – quadrat and transect by formed. using different techniques. (b) For Millon’s Reagent – A pinkish red To be done in groups. Use yellow and green colour is observed with mercuric pea seeds. Make a quadrat (30 cm X 30 cm) chloride. with blocks of 6 cm X 6 cm. Spread the seeds randomly on the table top. Put the quadrat (c) For Xanthoproteic Test: When and count the number of yellow and green concentrated nitric acid is boiled with peas per block; find the frequency of each protein a yellow colour is observed. On type of pea seed. addition of ammonium hydroxide or 14. Data collection – primary and secondary. liquor ammonia orange yellow precipitate is obtained. Collect any type of primary data and secondary data, tabulate the data and draw (iii)Lipids – Sudan III, Acrolein test, paper test conclusion. (a) Sudan III is a red fat-soluble dye used for identification of the presence of lipids, PROJECT WORK AND PRACTICAL FILE triglycerides and lipoproteins. It reacts – 15 marks with the lipids or triglycerides and gives Project Work – 10 Marks red colour. Candidates are to creatively execute one (b) Acrolein test is used to detect fat. When project/assignment on any aspect of Biotechnology. fat is heated strongly in the presence of Teachers may assign or students may choose any one potassium bisulphate/ sodium bisulphate project of their choice. The report should be kept (KHSO4/NaHSO4) that acts as a simple, but neat and elegant. dehydrating agent, the glycerol is Practical File – 5 Marks dehydrated to form an unsaturated aldehyde called acrolein that gives a Teachers are required to assess students on the basis pungent and irritating odour. of the practical file maintained by them during the academic year.

354 LIST OF ABBREVIATIONS 14. ICAR: Indian Council for Agricultural Research 1. CCMB:Centre for Cellular and Molecular 15. ICGEB: International Centre for Genetic Biology Engineering and Biotechnology 2. CdK: Cyclin dependent Kinase 16. ICMR: Indian Council for Medical Research 3. COV: Cross Over Value 17. IEF : Iso Electro Focussing 4. CSIR: Council of Scientific and Industrial 18. IPP: Intellectual Property Right Protection Act Research 19. IPR: Intellectual Property Right 5. DBT: Department of Biotechnology 20. IVF: In–Vitro Fertilization 6. DST: Department of Science and Technology 21. MALDI-MS: Matrix Assisted Laser Desorption 7. EFB: European Federation of Biotechnology Ionization – 22. MI: Mitotic Index 8. ELISA: Enzyme Linked Immuno Sorbent Assay 23. NADPH/NADP: Nicotinamide Adenine 9. ELSI: Ethical, Legal and Social Issues Dinucleotide Phosphate (reduced) / Nicotinamide 10. ETS/ETC: Electron Transport System / Electron Adenine Dinucleotide Phosphate Transport Cycle 24. NBTB: National Biotechnology Board 11. FMN/FAD: Flavin Mono Nucleotide / Flavin 25. OECD: Organization for Economic Cooperation Adenine Dinucleotide and Development 12. GEAC: Genetic Engineering Approval 26. PBR: Plant Breeder’s Right Committee 27. TPP: Thiamine Pyrophosphate 13. HLA: Human Leucocyte – associated Antigen 28. TSG: Tumour Suppressor Gene

355 CLASS XII

There will be two papers in the subject: Techniques of nucleic acid estimation – colorimetry and UV-visible Paper I: Theory……………. 3 hours ... 70 marks . Paper II: Practical…………. 3 hours ... 15 marks (ii) Protein Synthesis: synthesis of different Project Work………. …10 marks RNAs, and the complete mechanism of Practical File………… … 5 marks polypeptide chain formation. Concept of central dogma. PAPER I: THEORY- 70 Marks From genes to proteins: There will be one paper of three hours duration (a) Concept of transcriptional unit, divided into two parts. promoter, structural and terminator Part 1 (20 marks) will consist of compulsory short region; concept of split gene - intron and answer questions, testing knowledge, application and exon; monocistronic and polycistronic skills relating to elementary/fundamental aspects of RNA, hnRNA; the entire syllabus. (b) Transcription – explanation of the Part 2 (50 marks) will consist of eight questions out complete process including enzymes of which the candidates will be required to answer involved in the process; Post- five questions. Each question in this part shall carry transcriptional changes and their 10 marks. significance in eukaryotes – 1. Molecular Biology polyadenylation, capping and RNA splicing; (i) Nucleic acids and their estimation: an understanding of nucleic acids, their (c) Concept of reverse transcription; biochemical structure. (d) Genetic code – properties of genetic DNA as the genetic material (Hershey and code, start and stop codons, anticodons. Chase experiment). (e) The translation of RNA to protein – DNA (B-DNA)– physical and chemical complete mechanism of chain initiation, structure; definition, double helical model of elongation and termination, the role of DNA, (Watson and Crick’s); Nucleotide and tRNA, mRNA and rRNA in protein nucleoside; Chargaff’s Law, method of synthesis. (Post translational changes replication of DNA, various replicative not included). enzymes in both prokaryotic and eukaryotic (iii) Gene regulation in prokaryotes organisms, example topoisomerases, helicase, SSBs polymerases, primases, Operon concept – lac operon and trp ligases. Concept of semi conservative (with operon. respect to Messelson and Stahl experiment 2. Genetic Engineering and Taylor et.al experiment on Vicia faba (i) Introduction to gene cloning and genetic using radiolabelled thymidine) and semi- engineering: concept of cloning and vectors. discontinuous replication, (leading and lagging strands), okazaki fragments. Tools of recombinant DNA technology, types of restriction endonucleases and other RNA – definition, various types of RNAs such enzymes used in gene cloning; techniques as mRNA, tRNA (Clover leaf model with involved in extraction and purification of DNA diagram; brief introduction to L-shaped from bacterial, plant and animal cells. model), rRNA their structure and functions.

356 Selection of host cells: eukaryotic and (iii) Gene analysis techniques: various techniques prokaryotic. involved in recombinant DNA technology. Vectors: Characteristics and types such as DNA probes – definition and use. plasmids -pBR322, pUC (in pBR322- presence of two antibiotic resistant genes and in Low resolution mapping techniques: gel pUCpresence of lac Z gene to be taught), electrophoresis, southern blotting (details of cosmids, phages (M13 and λ), YACs, BACs (to the technique to be taught), western and be taught with reference to stability and their northern blotting (a brief idea and their carrying capacity), animal and plant viruses uses). (CaMV, retrovirus, SV40 – only names of High resolution techniques: DNA viruses, no details). sequencing- sequencing by chain Transfer of recombinants into host cells – termination, automated DNA sequencing. Site directed mutagenesis. (a) Vectorless methods - basic concept of transformation, transfection, DNA amplification by Polymerase chain electroporation, liposome mediated gene reaction (PCR)– applications of PCR, steps transfer, microinjection, biolistic and application of DNA profiling or DNA finger printing. (b) Vector-mediated method - Agrobacterium tumefaciens induced gene transfer. 3. Cell culture technology Methods of identification of recombinants- A brief idea of tools and techniques involved in Direct selection (green fluorescent cell culture technology and their applications in selection) and Insertional inactivation microbial, plant tissue and animal cell cultures (Blue-white selection, antibiotic respectively. resistance). (i) General tools and techniques used in cell A basic understanding of DNA libraries – culture technology construction of genomic and cDNA (a) Instruments - centrifuge, LAF hood and libraries. biosafety cabinets, pH meter, autoclave, Construction of a recombinant DNA vortex mixer, , magnetic molecule. stirrer, weighing balance, micro filtration unit, incubator, CO2 (ii) Innovations in Biotechnology: produced by incubator, inverted microscope, using modern biotechnological tools, (select bioreactor (diagram, its components examples of products already available) and their function)-stirred tank and sparged type (brief idea only), use of T (a) Plants: Production of Flavr Savr flasks to propagate animal cells. tomatoes, Bt-crops and Golden rice. Only uses of the above instruments to (b) Healthcare: Production of recombinant be studied. hepatitis-B vaccine, Humulin, interferon and edible vaccines. (b) Sterilization techniques for culture (c) Animal: Dolly the cloned sheep, Sources room, apparatus, transfer area, media, and characteristics of stem cells and vitamins, and living material; their applications. (c) Cryopreservation (need and steps). (d) Environmental biotechnology: (d) Cell counting (direct counting by bioremediation using oil-eating bacteria haemocytometer), cell viability by as an example. Evan’s blue stain and cell sorting (FACS only) (e) Industrial biotechnology: applications of industrial enzymes – rennet, subtilisin, amylase, papain. 357 (e) Types of media (synthetic /defined, (h) Biodegradable plastics (concept of PHB). semi-synthetic/differential, (iv) Animal cell culture and its application. complex/natural) Primary cell culture with mechanical and Preparation of media: microbial media- enzymatic disaggregation and its drawbacks; LB agar and LB broth; Plant media-MS Types of cell-lines: finite, continuous, and White’s media; Animal media- adherent and suspension; scale up-mono RPMI, DMEM and FBS - brief idea layer by Roller , application of animal cell culture-tissue, hybridoma technology, only. (includes inorganic and organic tissue engineering (definition only). macronutrients and micronutrients, 4. Bioinformatics antibiotics, growth regulators for (i) Introduction to bioinformatics; global plants: auxins and cytokinins). bioinformatics databases and data retrieval Importance of pH and solidifying agents. tools; genomics, different types of sequences, types of sequence analysis. (ii) Microbial culture and its application. Introduction to bioinformatics: definition and Fermentation process and growth kinetics- need. batch culture, fed batch culture, continuous An introduction to global bioinformatics culture (with the help of graphs only): databases (nucleotide and protein Definition of and : databases). Information sources such as Products and application-SCP (definition EMBL, NCBI, DDBJ, SWISSPROT, and use), industrial enzyme-subtilisin (source GenBank, GENSCAN. and its use). Data retrieval tools- ENTREZ, Taxonomy Browser. (iii) Plant tissue culture and its application. (ii) Genomics: Definition, introduction, tools used of single cell by mechanical and in Genomics and its applications. enzymatic methods, synchronisation of cell Definition of genomics. Types of genomics- culture by chemical methods like starvation, structural and functional. Basic criteria in inhibition and mitotic arrest. selecting the organism for its genome Cellular totipotency-definition of cellular sequencing. Different types of sequences – differentiation, de-differentiation, re- cDNA, genomic DNA, ESTs (Expressed differentiation. Application of plant cell Sequence Tags) and STSs (Sequence Tagged culture technology (methodology not Sites) and the different softwares (example required, only brief idea needed): gene scan). (a) Haploid production-androgenesis and Types of sequence analysis by using BLAST and FASTA –global, local, pair wise and gynogenesis and their significance. multiple. (b) Triploid production-understanding and Human Genome Project - its objectives, the need for triploid production and its countries involved, its achievements and application (seedless crops). significance. (c) In-vitro pollination- concept and its DNA microarray technology – definition and application. application only. (d) Zygotic embryo culture- concept and its Concept of Single Nucleotide Polymorphisms application, Embryo rescue (brief idea (SNPs). only). (iii) Proteomics: definition, introduction and (e) Somatic hybridisation-protoplast fusion databases. (Pomato). Types of Proteomics – structural, functional (f) Micropropagation and its significance. and expression; Important protein databases (g) Developing virus free plants and synthetic available for the public on the internet like seeds. PDB (Protein Data Bank), PIR (Protein Identification Resources). 358 PAPER II test tubes, plugged and kept in a tilted position (at an angle of 45o) until it sets. PRACTICAL WORK – 15 marks 6. Inoculation and incubation of Lactobacillus on Candidates are required to complete the following the culture medium in the Petri plate. experiments. Use of or inoculation needle for 1. Paper Chromatography – separation of the purpose. photosynthetic pigments 7. Identification of bacteria by Gram +ve and Gram Take any leaf. Extract chlorophyll in 80% –ve (from curd /saliva and/or soil solution) acetone. Take a strip of paper or prepare a thin layer of silica gel on a slide. Load chlorophyll (i) Prepare a bacterial smear on a slide (ii) Stain extract at one end of the paper/gel. Keep paper with crystal violet stain. (iii) Rinse with water. or gel in the rising medium in test tube or jar for (iv) Add a few drops of iodine solution. (v) Add about 30 minutes. The rising medium should few drops of 90 % ethanol (vi) Counterstain with have methanol/ acetic acid, n-butanol or safranin solution (vii) Observe the red and blue benzene. The rising fluid should always be at the colonies under the microscope bottom below the point of loading of 8. Action of enzymes on starch under: (a) variable chlorophylls. After 30 minutes, three spots: temperature (b) variable substrate concentration – yellow, bluish green and light green will be plotting of Km value by graph observed corresponding to carotenes, (i) Soluble starch solution (0.5% - 1%) to be chlorophyll A & chlorophyll B. prepared. Test with iodine. Collect saliva, 2. Preparation of buffers – phosphate, acetate and dilute 1: 5, add 1 ml of saliva to 10 ml of borate buffers starch solution. Incubate for 15 minutes. This experiment should be done to make the Again test for presence of starch with iodine. basics clear to the students. Basic calculation for Also test for the presence of reducing sugars buffer preparation should be known. The in solution. Repeat the same process at the approach should be to utilize easily available variable volumes of starch chemicals at reasonable costs. Phosphate, borate (ii) To study the effect of variable temperature on and acetate buffers can give the range of pH 4 - the activity of the enzyme salivary amylase. pH 9.2 9. Isolation of DNA from plants 3. Preparation of culture media Take half a ripe and peeled banana into a beaker (i) Bacterial culture Media - Luria Bertani and add 50 ml of extraction fluid (1.5gm table (L.B.) media - Peptone/ Tryptone, yeast salt +10 ml liquid detergent +90 ml distilled extract and NaCl. (Nutrient broth / Nutrient water). Place the beaker in a water bath set at 60 Agar). degrees C for 15 minutes. Stir gently with a glass (ii) Plant Tissue culture medium (Sugars + rod. Filter 5ml of cooled content into a clean test Coconut milk + Agar Agar). tube and add 5ml of cold 90% ethanol. DNA molecules separate out and appear as white 4. Sterilization of culture medium and other fibres. [DNA can also be extracted from pea materials. seeds and soaked wheat grains] (i) Dry Physical method – heat or radiation. 10. DNA estimation by colorimeter by DPA method. (ii) Wet Physical methods – steam sterilization. 11. Protein estimation by colour reaction – Bradford (iii) Chemical Sterilization/ Surface sterilization test. Disinfection with 70% alcohol and Sodium Bradford’s Assay is a Dye binding assay based hypochlorite solution carbolic acid on the differential change of colour of a dye in 5. Preparation of various forms of culture media – response to various concentrations of proteins. Petri plate, slant and suspension. Bradford’s assay can be performed for Luria Bertani (L.B) media to be prepared, qualitative as well as quantitative assessment of autoclaved and cooled to 60 degrees C. To proteins in a sample. prepare nutrient plates the media is poured into Dilute 1 volume of Bradford’s dye with 4 volumes presterilized petri-dishes under a LAF. To of distilled water. Filter the dye through prepare slants the media is poured into several Whatman and store at room 359 temperature in a brown glass bottle. Take A list of suggested projects is as follows: different aliquots of standard Bovine Serum 1. Effluent analysis. Albumin (BSA solution), for example (0.2, 0.4, 2. A study of the technological details of malt 0.6, 0.8 and 1.0 ml) in different test tubes Make preparation. up the volume to 1ml with distilled water. To 3. A study of the technological details of the each tube add 2ml of Bradford’s dye. Extent of brewing industry. colour development can be made by rough 4. A study of the organisation of a fermenter. estimate using + signs to show the concentration 5. Technological analysis of the process of drug of protein in the sample. Alternatively, OD can development, drug designing and drug targeting. be read using colorimeter or spectrophotometer. 6. A study of the technological details of vaccine Take the unknown sample to be estimated and development. perform the experiment. Similarly read the OD 7. Diagnosis of diseases by modern techniques like and note the corresponding concentration of ELISA, RIA and Antibody targeting. protein in it using the graph. 8. DNA finger-printing. 12. Cell viability test by Evan’s blue dye. 9. DNA foot-printing. 13. Isolation of milk protein – wet weight and dry 10. Microbiological contaminants in food and food weight. products. 11. Isolation of microbes from air, water and soil. Milk proteins are isolated by adding 0.4 N HCl 12. Methods of identifying microbes (various into the milk sample. Casein start coagulating at techniques and biochemical reactions). its isoelectric point (i.e. at pH 4.6). The precipitate is filtered and weighed to quantify the 13. Tissue Culture and its applications. protein present. 14. Stem Cell Technology 15. Nanotechnology 14. Chromatography to find adulteration in spices by 16. Bioinformatics using mixer of turmeric and metanil yellow. 17. Genetic Engineering 15. Demonstration of cell counting by 18. Cloning haemocytometer by using diluted blood. 19. Instrumentation in biotechnology 16. Experiment to show the process of 20. Forensic Biotechnology saponification. 21. Ethical, Legal and Social Issues (ELSI) related to Biotechnology/ GMOs PROJECT WORK AND PRACTICAL FILE 22. Biopiracy- Case Studies – 15 Marks Practical File – 5 Marks Project Work – 10 Marks The Visiting Examiner is required to assess students on the basis of the practical file maintained by them The Project Work is to be assessed by a Visiting during the academic year. Examiner appointed locally and approved by the Suggested Evaluation Criteria for Project Work: Council. Format of the Project: Candidates are to creatively execute one project / assignment on an aspect of Biotechnology. – Content – Introduction Teachers may assign or students may choose any one – Presentation (graphs, tables, charts, newspaper project of their choice. The report should be kept cuttings, diagrams, photographs, statistical simple, but neat and elegant. analysis if relevant) – Conclusion/ Summary – Bibliography

360 LIST OF EQUIPMENT FOR BIOTECHNOLOGY PRACTICALS FOR CLASSES XI & XII 1. Table-top Centrifuge 12. Incubator 2. Vortex - Mixer 13. with hot plate 3. Thermostatic water-bath 14. 4. Spectrophotometer (UV visible range)/ 15. Weighing Balance (Electrical) Colorimeter 16. Hot plate 5. Refrigerator 17. Binocular Microscope 6. Lactometer 18. Haemocytometer 7. pH meter 19. Colony counter 8. Hot air oven 20. Antiserum 9. Autoclave 21. Electrophoresis chamber 10. Desiccators 22. Micropipettes 11. Micro-filtration unit LIST OF ABBREVIATIONS TO BE STUDIED 1. BAC: Bacterial Artificial Chromosomes 21. NCBI: National Centre for Biotechnology 2. BLAST: Basic Local Alignment Search Tool Information 3. CTAB: Cetyl Trimethyl Ammonium Bromide 22. NHGRI: National Human Genome Research 4. DBM: Diazo–benzyl oxy–methyl paper Institute 5. DDBJ: DNA Database/ Data Bank of Japan 23. PAGE: Polyacrylamide Gel Electrophoresis 6. ddNTP: Dideoxy Nucleoside triphosphate 24. PCR: Polymerization Chain Reaction 7. DMEM: Dulbecco Modified Eagle Medium 25. PDB: Protein Database/ Data Bank 8. EBI: European Bioinformatics Institute 26. PHB: Poly 3–Hydroxyl Butyrate 9. EMBL: European Molecular Biology Laboratory 27. PIR: Protein Information Resource 10. EST: Expressed Sequence Tag 28. RFLP: Restriction Fragment Length 11. FACS: Fluorescence Activated Cell Sorting Polymorphism 12. FASTA: Fast All 29. RNA: Ribonucleic acid 13. FBS: Foetal Bovine Serum 30. RPMI medium: Roswell Park Memorial Institute 14. HEPA: High Energy Particulate Air medium 15. HGP: Human Genome Project 31. SCP: Single Cell Protein 16. IBPGR: International Board of Plant Genetic 32. SDS – PAGE: Sodium Dodecyl Sulphate– Resources Polyacrylamide Gel Electrophoresis 17. ICGEB: International Centre for Genetic 33. SNP: Single Nucleotide Polymorphism Engineering and Biotechnology 34. SSBs: Single Stranded Binding Proteins 18. IFN: Interferon 35. STS: Sequence Tagged Site 19. LB medium: Luria and Bertani Medium 36. VNTR: Variable Number of Tandem Repeats 20. MS medium: Murashige and Skoog medium 37. YAC: Yeast Artificial Chromosome

361