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(12) United States Patent (10) Patent No.: US 8,633,353 B2 Ratcliffe Et Al

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(12) United States Patent (10) Patent No.: US 8,633,353 B2 Ratcliffe Et Al USOO8633353B2 (12) United States Patent (10) Patent No.: US 8,633,353 B2 Ratcliffe et al. (45) Date of Patent: Jan. 21, 2014 (54) PLANTS WITH IMPROVED WATER DEFICIT PCT/US2004/037584, filed on Nov. 12, 2004, which is AND COLD TOLERANCE a continuation-in-part of application No. 10/714,887, application No. 1 1/981,813, which is a (75) Inventors: Oliver . Ratcliffe, Oakland, CA (US); continuation-in-part of application No. Jacqueline Heard, Webster Groves, MO PCT/US2006/034615, filed on Aug. 31, 2006, and a (US); Rodrick W. Kumimoto, Norman, continuation-in-part of application No. 1 1/705,903, OK (US); Peter P. Repetti, Emeryville, filed on Feb. 12, 2007, now Pat. No. 7,868,229, which CA (US); T. Lynne Reuber, San Mateo, is a continuation-in-part of application No. CA (US); Robert Creelman, Castro PCT/US2006/034615 Valley, CA (US); Frederick D. Hempel, Sunol, CA (US); Neal L. Gutterson, (60) Provisional application No. 60/961,403, filed on Jul. Oakland, CA (US); Roger Canales, 20, 2007, provisional application No. 60/713,952, Bernburg (DE) filed on Aug. 31, 2005. (73) Assignee: Mendel Biotechnology, Inc., Hayward, (51) Int. Cl. CA (US) AOIH I/O (2006.01) c - r - (52) U.S. Cl. (*) Notice: patentSubject is to extended any disclaimer, or adjusted the term under of this 35 USPC ............ 800/278; 800/287: 800/289: 800/290 U.S.C. 154(b) by 753 days. (58) Field of Classification Search None (21) Appl. No.: 11/981,813 See application file for complete search history. (22) Filed: Oct. 30, 2007 (56) References Cited (65) Prior Publication Data U.S. PATENT DOCUMENTS US 2008/O163397 A1 Jul. 3, 2008 6,121,513 A 9/2000 Zhang et al. 6,235,975 B1 5, 2001 Harada et al. Related U.S. ApplicationO O Data 6,476,2126,320,102 B1 11/20021 1/2001 HaradaLalgudi etet al. al. (60) Continuation-in-part of application No. 10/675,852, g: R 3: Sinces al. - I - aala Ca. continuation-in-partfiled on Sep. 30, 2003,of application now abandoned, No. 1 1/479,226, and a 6,664,446 B2 12/2003 Heard et al. filed on Jun. 30, 2006, now Pat. No. 7,858,848, and a (Continued) continuation-in-part of application No. 1 1/725,235, filed on Mar. 16, 2007, now Pat. No. 7,601,893, and a FOREIGN PATENT DOCUMENTS continuation-in-part of application No. 1 1/728,567, AR PO70 102784 1, 2008 filed on Mar. 26, 2007, now Pat. No. 7,635,800, and a CA 2302828 10, 2000 continuation-in-part of application No. 1 1/069.255, (Continued) filed on Feb. 28, 2005, now Pat. No. 8,558,059, and a continuation-in-part of application No. 10/374,780, OTHER PUBLICATIONS filed on Feb. 25, 2003, now Pat. No. 7,511,190, and a continuation-in-part of application No. 10/546,266, Bowie et al, Science 247: 1306-1310, 1990.* filed as application No. PCT/US2004/005654 on Feb. 25, 2004, now Pat. No. 7,659,446, which is a (Continued) continuation-in-part of application No. 10/374,780, and a continuation-in-part of application No. Primary Examiner — Stuart F Baum 10/675,852, application No. 1 1/981,813, which is a (74) Attorney, Agent, or Firm — Jeffrey M. Libby: Yifan continuation-in-part of application No. 10/412,699, Mao filed on Apr. 10, 2003, now Pat. No. 7,345,217, which is a continuation-in-part of application No. (57) ABSTRACT 10/374,780, application No. 1 1/981,813, which is a The present invention provides nucleic acid constructs, continuation-in-part of application No. 1 1/642,814, including plasmids, expression vectors or expression cas filed on Dec. 20, 2006, now Pat. No. 7,825,296, which settes comprising polynucleotides encoding CCAAT-binding is a division of application No. 10/666,642, filed on transcription factor polypeptides that have the ability to Sep. 18, 2003, now Pat. No. 7,196,245, application No. increase a plants tolerance to abiotic stress. Polynucleotides 11/981,813, which is a continuation-in-part of encoding functional CCAAT-binding transcription factors application No. 10/714,887, filed on Nov. 13, 2003, were incorporated into expression vectors, introduced into now abandoned, which is a continuation-in-part of plants, and ectopically expressed. The encoded polypeptides application No. 10/666,642, application No. of the invention significantly increased the cold and water 11/981,813, which is a continuation-in-part of deficit tolerance of the transgenic plants, as compared to application No. 11/435,388, filed on May 15, 2006, tolerance to these stresses of control plants. now Pat. No. 7,663,025, which is a continuation-in-part of application No. 22 Claims, 5 Drawing Sheets US 8,633,353 B2 Page 2 (56) References Cited 2009,0183270 A1 7/2009 Adams 2009,0192305 A1 7/2009 Riechmann et al. 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