1

Genetics and Molecular Biology

Suplementary material to: Draft genome of Thermomonospora sp. CIT 1 (Thermomonosporaceae) and in silico evidence of its functional role in filter cake biomass deconstruction

Table S3 - Identifications of with activity on carbohydrate structures present in the draft genome CIT 1 recovered from metagenomic sequencing of filter cake. Predictions were performed with dbCAN online, following for blastp confirmation against the non-redundant NCBI protein database of the occurrence of predicted protein-like sequence deposition.

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy NAME (NCBI) ACCESSION FAMILY COVER VALUE Y 747 AA2.hmm peroxidase (EC 1.11.1.-) catalase/peroxidase HPI 100 0.00E+00 100 WP_012852289 -methanol-choline 786 AA3.hmm glucose-methanol-choline (GMC) 100 0.00E+00 100 ACY97563 mycofactocin system GMC family 520 AA3.hmm glucose-methanol-choline (GMC) 99 0.00E+00 99 WP_012852714 oxidoreductase MftG 582 AA3.hmm glucose-methanol-choline (GMC) GMC family oxidoreductase 100 0.00E+00 99 WP_012854558 531 AA3_2.hmm glucose-methanol-choline (GMC) choline dehydrogenase 100 0.00E+00 100 WP_012854223 533 AA4.hmm vanillyl-alcohol oxidase (EC 1.1.3.38) FAD-binding oxidoreductase 99 0.00E+00 99 WP_012852156 NAD(P)H:quinone oxidoreductase 4.00E- 209 AA6.hmm 1,4-benzoquinone reductase (EC. 1.6.5.6) 100 100 WP_012850533 type IV 152 187 AA6.hmm 1,4-benzoquinone reductase (EC. 1.6.5.6) NAD(P)H-dependent oxidoreductase 100 9.00E-86 69 WP_067443322 6.00E- 152 AA6.hmm 1,4-benzoquinone reductase (EC. 1.6.5.6) flavodoxin 99 100 WP_012853676 104 3.00E- 178 AA6.hmm 1,4-benzoquinone reductase (EC. 1.6.5.6) NADPH-dependent oxidoreductase 100 99 WP_012855187 124 457 AA7.hmm 1- FAD-binding oxidoreductase 100 0.00E+00 100 WP_012850500 441 AA7.hmm - FAD-linked oxidoreductase 100 0.00E+00 100 WP_012852047 FAD linked oxidase domain- 378 AA7.hmm - 100 0.00E+00 99 WP_012852317 containing protein 459 AA7.hmm - FAD-binding oxidoreductase 100 0.00E+00 99 WP_012853068 2

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y 502 AA7.hmm - FAD-binding oxidoreductase 92 0.00E+00 99 WP_012853288 482 AA7.hmm - FAD-binding oxidoreductase 100 0.00E+00 99 WP_012853594 411 AA7.hmm - FAD-binding oxidoreductase 100 0.00E+00 99 WP_012853595 lytic polysaccharide monooxygenases 8.00E- 188 AA10.hmm chitin-binding domain 3 protein 100 90 ACZ00089 (LPMOs) 109 2.00E- 159 CBM2.hmm CBM2 cellulose-binding family II 100 100 WP_012853764 109 447 CBM2.hmm CBM2 cellulose-binding family II 99 0.00E+00 99 ACY99074 335 CBM13.hmm - hypothetical protein Tcur_3315 100 0.00E+00 99 ACY98853 coagulation factor 5/8 type domain 1430 CBM32.hmm - 100 0.00E+00 99 ACY99521 protein 532 CBM32.hmm - phosphopeptide-binding protein 100 0.00E+00 100 WP_012855260 498 CBM35.hmm CBM35+GH43 glycoside family 43 100 0.00E+00 99 WP_012851007 918 CBM44.hmm - PKD domain-containing protein 100 0.00E+00 99 WP_012851385 701 CBM48.hmm - glycogen debranching enzyme GlgX 100 0.00E+00 100 WP_012852133 736 CBM48.hmm - 1,4-alpha-glucan-branching protein 100 0.00E+00 99 WP_012853921 680 CBM48.hmm - glycogen debranching protein 100 0.00E+00 99 WP_012854434 280 CE1.hmm esterase esterase 100 0.00E+00 99 WP_012851583 288 CE1.hmm esterase esterase 100 0.00E+00 100 WP_012850408 284 CE1.hmm esterase alpha/beta hydrolase fold protein 100 0.00E+00 99 ACY97135 347 CE1.hmm esterase alpha/beta hydrolase 91 0.00E+00 99 WP_012852003 362 CE1.hmm - hypothetical protein 100 0.00E+00 99 WP_052305466 9.00E- 309 CE1.hmm esterase alpha/beta hydrolase fold protein 72 99 WP_012852600 154 278 CE1.hmm cinnamoyl esterase (EC 3.1.1.-) putative lipoprotein 100 0.00E+00 99 WP_012854167 292 CE1.hmm cinnamoyl esterase (EC 3.1.1.-) lipase 100 0.00E+00 99 WP_012850775 263 CE1.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012854209 3

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y 364 CE1.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012854576 374 CE1.hmm esterase putative esterase 100 0.00E+00 100 ACY96221 SGNH/GDSL hydrolase family 253 CE2.hmm - 100 0.00E+00 99 WP_012851045 protein 343 CE3.hmm - GDSL family lipase 100 0.00E+00 100 WP_012851417 267 CE4.hmm - polysaccharide deacetylase 100 0.00E+00 99 WP_012851516 341 CE4.hmm - polysaccharide deacetylase 100 0.00E+00 99 ACY97622 375 CE4.hmm - polysaccharide deacetylase 100 0.00E+00 98 WP_012853180 polysaccharide deacetylase family 285 CE4.hmm - 100 0.00E+00 100 WP_012853293 protein 264 CE4.hmm - polysaccharide deacetylase 100 0.00E+00 99 WP_012853908 4.00E- 234 CE4.hmm - polysaccharide deacetylase 100 100 ACZ00196 168 polysaccharide deacetylase family 275 CE4.hmm - 100 0.00E+00 99 WP_012855020 protein 5.00E- 235 CE5.hmm cutinase (EC 3.1.1.74) cutinase family protein 100 99 WP_012851874 164 4.00E- 241 CE5.hmm cutinase (EC 3.1.1.74) cutinase 100 99 ACY97091 175 6.00E- 176 CE5.hmm cutinase (EC 3.1.1.74) cutinase family protein 100 99 WP_012851879 123 N-acetylglucosamine 6-phosphate deacetylase N-acetylglucosamine-6-phosphate 410 CE9.hmm 100 0.00E+00 99 WP_012854344 (EC 3.5.1.25) deacetylase Alpha/beta hydrolase fold-3 domain 349 CE10.hmm esterase 100 0.00E+00 100 ACY96637 protein 335 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012851463 692 CE10.hmm - S9 family peptidase 100 0.00E+00 99 WP_012851498 703 CE10.hmm - S9 family peptidase 100 0.00E+00 100 WP_012851705 593 CE10.hmm cinnamoyl esterase (EC 3.1.1.-) Esterase/lipase-like protein 100 0.00E+00 99 ACY97597 317 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012852810 4

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y 323 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012852854 489 CE10.hmm - peptidase S15 100 0.00E+00 99 WP_012852916 546 CE10.hmm carboxyl esterase (EC 3.1.1.3) carboxylesterase family protein 100 0.00E+00 99 WP_012850644 alpha/beta hydrolase fold-3 domain- 2.00E- 234 CE10.hmm esterase 100 100 WP_012853073 containing protein 158 668 CE10.hmm - S9 family peptidase 100 0.00E+00 100 WP_012853613 343 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 99 WP_012854310 364 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012854576 362 CE10.hmm esterase alpha/beta hydrolase 99 0.00E+00 99 WP_083789919 587 CE10.hmm - S9 family peptidase 100 0.00E+00 99 WP_012855196 303 CE14.hmm mycothiol S-conjugate amidase (EC 3.5.1.-) mycothiol conjugate amidase Mca 100 0.00E+00 100 ACY96652 N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy- 1D-myo-inositol 2-acetamido-2- 297 CE14.hmm α-D-glucopyranoside deacetylase (EC deoxy-alpha-D-glucopyranoside 100 0.00E+00 100 WP_012851500 3.5.1.89) deacetylase N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy- 271 CE14.hmm α-D-glucopyranoside deacetylase (EC GlcNAc-PI de-N-acetylase 100 0.00E+00 100 WP_041441415 3.5.1.89) 482 GH1.hmm β-glucosidase (EC 3.2.1.21) beta-glucosidase 100 0.00E+00 100 WP_012852091 471 GH1.hmm β-glucosidase (EC 3.2.1.21) beta-glucosidase 100 0.00E+00 100 WP_012852534 447 GH1.hmm β-glucosidase (EC 3.2.1.21) beta-glucosidase 100 0.00E+00 99 WP_012855073 family 3 domain- 543 GH3.hmm - 100 0.00E+00 100 WP_012854338 containing protein 427 GH4.hmm - glycoside hydrolase family 4 100 0.00E+00 99 WP_012854171 626 GH5_43.hmm endoglycoceramidase (EC 3.2.1.123) 98 0.00E+00 99 WP_012851730 409 GH6.hmm - glycoside hydrolase family 6 100 0.00E+00 99 WP_012852096 701 GH13_11.hmm - glycogen debranching enzyme GlgX 100 0.00E+00 100 WP_012852133 680 GH13_11.hmm - glycogen debranching protein 100 0.00E+00 99 WP_012854434 613 GH13_16.hmm - maltose alpha-D-glucosyltransferase 100 0.00E+00 99 WP_083790013 5

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y α-1,4-glucan: phosphate α-maltosyltransferase alpha-1,4-glucan-maltose-1-phosphate 661 GH13_3.hmm 100 0.00E+00 99 WP_012853902 (EC 2.4.99.16) maltosyltransferase 550 GH13_30.hmm α- (EC 3.2.1.1) alpha-amylase 100 0.00E+00 99 WP_012852097 736 GH13_9.hmm branching enzyme (EC 2.4.1.18) 1,4-alpha-glucan-branching protein 100 0.00E+00 99 WP_012853921 629 GH15.hmm - glycoside hydrolase family 15 protein 100 0.00E+00 100 WP_012853541 484 GH15.hmm - glycoside hydrolase family 15 100 0.00E+00 100 WP_012854532 272 GH23.hmm - hypothetical protein 100 0.00E+00 100 WP_012851447 352 GH23.hmm - NLP/P60 protein 99 0.00E+00 100 WP_012851472 peptidoglycan (EC 4.2.2.n1) also known lytic transglycosylase domain- 378 GH23.hmm in the literature as peptidoglycan lytic 100 0.00E+00 99 WP_012852049 containing protein transglycosylase 8.00E- 246 GH23.hmm - hypothetical protein 100 99 WP_012852903 180 peptidoglycan lyase (EC 4.2.2.n1) also known transglycosylase domain-containing 266 GH23.hmm in the literature as peptidoglycan lytic 100 0.00E+00 100 WP_012851352 protein transglycosylase 297 GH25.hmm - glycoside hydrolase family 25 100 0.00E+00 100 WP_012852705 371 GH25.hmm - glycoside hydrolase family 25 100 0.00E+00 99 WP_012850936 334 GH26.hmm β-mannanase (EC 3.2.1.78) beta-mannanase-like protein 100 0.00E+00 99 WP_012854497 335 GH26.hmm - hypothetical protein 100 0.00E+00 100 WP_012851356 506 GH26.hmm - hypothetical protein 100 0.00E+00 100 WP_012851387 465 GH30.hmm - hypothetical protein 100 0.00E+00 99 WP_012853919 973 GH33.hmm - hypothetical protein 100 0.00E+00 100 WP_012853982 498 GH43_30.hmm - glycoside hydrolase family 43 100 0.00E+00 99 WP_012851007 arabinan endo-1,5-alpha-L- 331 GH43_5.hmm arabinanase (EC 3.2.1.99) 100 0.00E+00 100 WP_012853142 arabinosidase 456 GH63.hmm - hypothetical protein 100 0.00E+00 100 WP_012852353 440 GH63.hmm - hypothetical protein 100 0.00E+00 99 WP_012853510 6

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y 810 GH65.hmm - glycoside hydrolase family 65 protein 100 0.00E+00 100 WP_012853146 794 GH65.hmm - glycoside hydrolase family 65 protein 100 0.00E+00 99 WP_012853851 amylomaltase or 4-α-glucanotransferase (EC 717 GH77.hmm 4-alpha-glucanotransferase 100 0.00E+00 99 WP_012851713 2.4.1.25) 303 GH117.hmm - hypothetical protein 93 0.00E+00 99 WP_012851745 7.00E- 214 GH135.hmm - Zn-dependent hydrolase 100 100 WP_012854286 155 400 GT1.hmm - glycosyltransferase 99 0.00E+00 96 WP_012852871 protein of unknown function 380 GT1.hmm - 100 0.00E+00 100 ACY98322 DUF1205 380 GT1.hmm - hypothetical protein 100 0.00E+00 100 WP_012853119 418 GT1.hmm - glycosyltransferase 100 0.00E+00 99 WP_012855060 CDP-glycerol-- 946 GT2.hmm - poly(glycerophosphate) 100 0.00E+00 99 WP_012851579 glycerophosphotransferase 807 GT2.hmm - AMP-dependent synthetase and 100 0.00E+00 99 ACY97654 polyprenol monophosphomannose 1.00E- 248 GT2.hmm - 100 100 WP_012852699 synthase 178 487 GT2.hmm - glycosyl family 2 100 0.00E+00 99 ACY97924 2.00E- 238 GT2.hmm - glycosyltransferase family 2 protein 100 99 WP_012853905 172 337 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 99 WP_012853907 3.00E- 246 GT2.hmm - glycosyl transferase family 2 100 99 ACY99186 178 268 GT2.hmm - glycosyl transferase family 2 protein 99 0.00E+00 100 WP_012854307 1072 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 99 WP_012854382 305 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 100 WP_012854405 CDP-glycerol-- 1148 GT2.hmm - poly(glycerophosphate) 100 0.00E+00 99 WP_012854410 glycerophosphotransferase 317 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 99 WP_012854417 7

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y 324 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 99 WP_012854425 537 GT2.hmm - glycosyl transferase family 2 100 0.00E+00 100 ACY99713 418 GT2.hmm - polysaccharide synthesis protein GtrA 100 0.00E+00 100 WP_012850821 306 GT2.hmm - glycosyl transferase 100 0.00E+00 100 WP_012851004 658 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 99 WP_012851015 321 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 100 WP_012851019 CDP-glycerol:glycerophosphate 1176 GT2.hmm - 98 0.00E+00 99 WP_012851021 glycerophosphotransferase 430 GT2.hmm - glycosyl transferase family 2 100 0.00E+00 100 ACY96562 294 GT2.hmm - glycosyltransferase family 2 protein 100 0.00E+00 99 WP_012851375 390 GT4.hmm - glycosyltransferase family 4 protein 100 0.00E+00 100 WP_012851578 380 GT4.hmm - group 1 glycosyl transferase protein 100 0.00E+00 99 WP_012852245 388 GT4.hmm - glycosyltransferase family 1 protein 100 0.00E+00 100 WP_012852452 381 GT4.hmm - glycosyl transferase group 1 100 0.00E+00 100 ACY97742 425 GT4.hmm - glycosyltransferase family 4 protein 100 0.00E+00 96 WP_012852806 381 GT4.hmm - glycosyl transferase 100 0.00E+00 100 WP_012853078 374 GT4.hmm - glycosyltransferase family 1 protein 100 0.00E+00 100 WP_012853385 456 GT4.hmm - glycosyltransferase family 1 protein 99 0.00E+00 99 WP_012853975 381 GT4.hmm - glycosyltransferase family 1 protein 100 0.00E+00 99 WP_012854306 359 GT4.hmm - glycosyl transferase 100 0.00E+00 98 WP_012854404 418 GT4.hmm - glycosyltransferase family 4 protein 100 0.00E+00 100 WP_012854550 401 GT4.hmm - glycosyltransferase family 4 protein 92 0.00E+00 99 WP_012851010 D-inositol-3-phosphate 403 GT4.hmm - 100 0.00E+00 100 WP_012851072 glycosyltransferase 404 GT4.hmm - glycosyltransferase family 1 protein 100 0.00E+00 99 WP_012850472 283 GT14.hmm - glycosyl transferase family 14 100 0.00E+00 99 WP_012851388 8

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y α,α-trehalose-phosphate synthase [UDP- Alpha,alpha-trehalose-phosphate 470 GT20.hmm 100 0.00E+00 100 ACY97534 forming] (EC 2.4.1.15) synthase (UDP- forming) 493 GT20.hmm trehalose-6-P phosphatase (EC 3.1.3.12) trehalose-6-phosphate synthase 100 0.00E+00 99 WP_012851175 259 GT26.hmm - glycosyltransferase 100 0.00E+00 100 WP_012851389 UDP-GlcNAc: Und-PP-MurAc-pentapeptide undecaprenyldiphospho- 364 GT28.hmm β-N-acetylglucosaminyltransferase (EC muramoylpentapeptide beta-N- 100 0.00E+00 100 WP_012853252 2.4.1.227) acetylglucosaminyltransferase 863 GT35.hmm - DUF3417 domain-containing protein 100 0.00E+00 100 WP_012853901 496 GT39.hmm - hypothetical protein 100 0.00E+00 99 WP_012853866 phospholipid carrier-dependent 533 GT39.hmm - 99 0.00E+00 100 WP_012851342 glycosyltransferase 561 GT39.hmm - hypothetical protein 100 0.00E+00 99 WP_012851347 1189 GT51.hmm - glycosyl transferase family 51 99 0.00E+00 93 ACY96851 762 GT51.hmm murein polymerase (EC 2.4.1.129) peptidoglycan glycosyltransferase 98 0.00E+00 100 WP_012855218 817 GT51.hmm murein polymerase (EC 2.4.1.129) penicillin-binding protein 91 0.00E+00 99 WP_012855252 796 GT51.hmm - glycosyl transferase family 51 97 0.00E+00 99 WP_012851396 401 GT76.hmm - hypothetical protein Tcur_4954 99 0.00E+00 99 ACZ00470 6.00E- 236 GT81.hmm - glycosyl transferase family 2 100 99 WP_012850508 162 350 GT81.hmm - glycosyl transferase family 2 95 0.00E+00 99 ACY98049 mechanosensitive ion channel family 5.00E- 257 GT83.hmm - 100 99 WP_012854384 protein 175 504 GT83.hmm - hypothetical protein 100 0.00E+00 100 WP_012854498 746 GT83.hmm - glycosyl transferase 100 0.00E+00 99 WP_012850819 691 GT83.hmm - glycosyl transferase family 39 100 0.00E+00 94 WP_012850820 500 GT83.hmm - hypothetical protein 100 0.00E+00 99 WP_012851348 429 GT87.hmm - DUF2029 domain-containing protein 100 0.00E+00 100 WP_012850510

404 GT94.hmm - glycosyltransferase family 1 100 0.00E+00 99 WP_012850472 9

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY E- IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) ACCESSION FAMILY COVER VALUE Y

327 PL1.hmm pectate lyase (EC 4.2.2.2) pectate lyase 100 0.00E+00 99 WP_012852783 676 PL22_1.hmm - hypothetical protein 100 0.00E+00 99 WP_086014605 1 The name of the enzyme differs between the CAZy databases and non-redundant NCBI proteins.

Table S4 - Identifications of enzymes with activity on carbohydrate structures present in the circular genome of Thermomonospora curvata DSM 43183 (Thermomonosporaceae). Predictions were performed with dbCAN online, following for blastp confirmation against the non-redundant NCBI protein database of the occurrence of predicted protein-like sequence deposition.

Search results for dbCAN conserved domains BLASTP RESULTS ORF CAZy QUERY IDENTIT LENGTH ID CAZy ENZYME NAME (NCBI) E-VALUE ACCESSION FAMILY COVER Y mycofactocin system GMC family 517 AA3.hmm glucose-methanol-choline (GMC) 100 0.00E+00 100 WP_012852714 oxidoreductase MftG 528 AA4.hmm vanillyl-alcohol oxidase (EC 1.1.3.38) FAD-binding oxidoreductase 99 0.00E+00 76 WP_067912508 NAD(P)H dehydrogenase 209 AA6.hmm 1,4-benzoquinone reductase (EC. 1.6.5.6) 99 4.00E-128 88 SEG76937 (quinone) NAD(P)H-dependent FMN 186 AA6.hmm 1,4-benzoquinone reductase (EC. 1.6.5.6) 100 2.00E-84 70 SEG82383 reductase glycolate oxidase FAD binding 411 AA7.hmm glycolate oxidase (EC 1.1.3.15) 98 0.00E+00 77 SEG60993 subunit 482 AA7.hmm glycolate oxidase (EC 1.1.3.15) glycolate oxidase 100 0.00E+00 88 SEG60983 459 AA7.hmm 1- FAD-binding oxidoreductase 99 0.00E+00 72 WP_091378734 lytic polysaccharide monooxygenases 212 AA10.hmm chitin-binding protein 100 7.00E-86 73 WP_079318887 (LPMOs) 505 CBM35.hmm CBM35 CBM35+GH43 96 3.00E-172 57 SEG79516 943 CBM44.hmm - PKD domain containing protein 95 0.00E+00 66 WP_083597587 1,4-alpha-glucan branching 736 CBM48.hmm - 98 0.00E+00 86 SEF80265 enzyme 92 CBM48.hmm - hypothetical protein 98 7.00E-45 75 WP_030510813 292 CE1.hmm cinnamoyl esterase (EC 3.1.1.-) lipase 100 0.00E+00 100 WP_012850775 10

365 CE1.hmm - hypothetical protein 99 0.00E+00 99 WP_052305466 S-formylglutathione hydrolase (EC S-formylglutathione hydrolase 341 CE1.hmm 99 0.00E+00 78 SEG71481 3.1.2.12) FrmB 284 CE1.hmm esterase alpha/beta hydrolase 100 0.00E+00 99 WP_041439411 336 CE1.hmm - polyhydroxybutyrate depolymerase 96 2.00E-171 76 SEF89583 Pimeloyl-ACP methyl ester 317 CE1.hmm esterase 100 0.00E+00 85 SEG14212 carboxylesterase polysaccharide deacetylase family 285 CE4.hmm - 100 0.00E+00 100 WP_012855020 protein 267 CE4.hmm - polysaccharide deacetylase 100 0.00E+00 100 WP_012851516 264 CE4.hmm - polysaccharide deacetylase 100 0.00E+00 100 WP_012853908 polysaccharide deacetylase family 285 CE4.hmm - 100 0.00E+00 100 WP_012853293 protein 241 CE5.hmm cutinase (EC 3.1.1.74) cutinase 100 9.00E-176 100 ACY97091 alpha/beta hydrolase fold-3 234 CE10.hmm esterase 100 2.00E-158 100 WP_012853073 domain-containing protein 349 CE10.hmm - hypothetical protein 98 0.00E+00 99 WP_083789966 497 CE10.hmm - peptidase S15 100 0.00E+00 100 WP_012852916 668 CE10.hmm - S9 family peptidase 100 0.00E+00 100 WP_012853613 343 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012854310 317 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 99 WP_083789919 335 CE10.hmm esterase alpha/beta hydrolase 100 0.00E+00 100 WP_012851463 mycothiol S-conjugate amidase (EC 3.5.1.- 303 CE14.hmm mycothiol conjugate amidase Mca 97 0.00E+00 100 WP_041439331 ) N-acetyl-1-D-myo-inosityl-2-amino-2- 271 CE14.hmm deoxy-α-D-glucopyranoside deacetylase GlcNAc-PI de-N-acetylase 100 0.00E+00 100 WP_041441415 (EC 3.5.1.89) N-acetyl-1-D-myo-inosityl-2-amino-2- 1D-myo-inositol 2-acetamido-2- 297 CE14.hmm deoxy-α-D-glucopyranoside deacetylase deoxy-alpha-D-glucopyranoside 100 0.00E+00 100 WP_012851500 (EC 3.5.1.89) deacetylase 411 GH1.hmm β-glucosidase (EC 3.2.1.21) β-glucosidase 100 0.00E+00 100 WP_012852534 447 GH1.hmm β-glucosidase (EC 3.2.1.21) β-glucosidase 100 0.00E+00 100 WP_012855073 427 GH4.hmm - glycoside hydrolase family 4 100 0.00E+00 100 WP_012854171 11

618 GH5_43.hmm (EC 3.2.1.45) endoglycosylceramidase 100 0.00E+00 100 WP_012851730 419 GH6.hmm - glycoside hydrolase family 6 100 0.00E+00 100 WP_012852096 601 GH13_16.hmm - maltose α-D-glucosyltransferase 100 0.00E+00 99 WP_083790013 α-1,4-glucan: phosphate α- α-1,4-glucan--maltose-1-phosphate 684 GH13_3.hmm 100 0.00E+00 100 WP_012853902 maltosyltransferase (EC 2.4.99.16) maltosyltransferase 736 GH13_9.hmm - 1,4-α-glucan-branching protein 100 0.00E+00 100 WP_012853921 629 GH15.hmm - glycoside hydrolase family 15 100 0.00E+00 100 WP_012853541 484 GH15.hmm - glycoside hydrolase family 15 100 0.00E+00 100 WP_012854532 peptidoglycan lyase (EC 4.2.2.n1) also known in the literature as peptidoglycan lytic transglycosylase catalytic 378 GH23.hmm 100 0.00E+00 100 WP_012852049 lytic transglycosylase; (EC subunit 3.2.1.14) 349 GH23.hmm - NLP/P60 protein 100 0.00E+00 100 WP_012851472 peptidoglycan lyase (EC 4.2.2.n1) also known in the literature as peptidoglycan transglycosylase domain- 266 GH23.hmm 100 0.00E+00 100 WP_012851352 lytic transglycosylase; chitinase (EC containing protein 3.2.1.14) 272 GH23.hmm - hypothetical protein 100 0.00E+00 100 WP_012851447 380 GH25.hmm - glycoside hydrolase family 25 100 0.00E+00 100 WP_012850936 297 GH25.hmm - glycoside hydrolase family 25 100 0.00E+00 100 WP_012852705 335 GH26.hmm - hypothetical protein 100 0.00E+00 100 WP_012851356 334 GH26.hmm β-mannanase (EC 3.2.1.78) beta-mannanase-like protein 100 0.00E+00 100 WP_012854497 465 GH30.hmm - hypothetical protein 100 0.00E+00 100 WP_012853919 505 GH43_30.hmm - glycoside hydrolase family 43 100 0.00E+00 100 WP_012851007 456 GH63.hmm - hypothetical protein 100 0.00E+00 100 WP_012852353 807 GH65.hmm - glycoside hydrolase family 65 100 0.00E+00 100 WP_012855093 810 GH65.hmm - glycosyl hydrolase 100 0.00E+00 100 WP_012853146 794 GH65.hmm - glycoside hydrolase family 65 100 0.00E+00 100 WP_012853851 amylomaltase or 4-α-glucanotransferase 717 GH77.hmm 4-alpha-glucanotransferase 100 0.00E+00 100 WP_012851713 (EC 2.4.1.25) 283 GH117.hmm - hypothetical protein 100 0.00E+00 100 WP_012851745 12

DUF1205 domain-containing 380 GT1.hmm - 98 0.00E+00 100 WP_083789857 protein 418 GT1.hmm - glycosyltransferase 100 0.00E+00 100 WP_012855060 glycosyltransferase family 2 272 GT2.hmm - 100 0.00E+00 100 WP_012853905 protein glycosyltransferase family 2 337 GT2.hmm - 100 0.00E+00 100 WP_012853907 protein CDP-glycerol:glycerophosphate 157 GT2.hmm - 100 0.00E+00 100 WP_012851021 glycerophosphotransferase polyprenol monophosphomannose 248 GT2.hmm - 100 1.00E-178 100 WP_012852699 synthase Acyl-CoA synthetase (AMP- 807 GT2.hmm - 97 0.00E+00 86 SEG89133 forming)/AMP-acid ligase II glycosyltransferase family 2 301 GT2.hmm - 100 0.00E+00 100 WP_012851375 protein 537 GT2.hmm - glycosyl transferase family 2 87 0.00E+00 99 WP_041442530 306 GT2.hmm - glycosyl transferase 95 0.00E+00 90 SEG79500 glycosyltransferase family 2 321 GT2.hmm - 100 0.00E+00 100 WP_012851019 protein 315 GT2.hmm - glycosyl transferase 100 0.00E+00 100 WP_012851009 CDP-glycerol-- 1148 GT2.hmm - poly(glycerophosphate) 100 0.00E+00 100 WP_012854410 glycerophosphotransferase 658 GT2.hmm - glycosyl transferase 100 0.00E+00 100 WP_012851015 glycosyltransferase family 2 430 GT2.hmm - 98 0.00E+00 100 WP_083789964 protein 381 GT4.hmm - glycosyl transferase 100 0.00E+00 100 WP_012853078 glycosyltransferase family 1 388 GT4.hmm - 100 0.00E+00 100 WP_012852452 protein glycosyltransferase family 1 381 GT4.hmm - 98 0.00E+00 99 WP_041441526 protein glycosyltransferase family 4 443 GT4.hmm - 100 0.00E+00 100 WP_012852806 protein D-inositol-3-phosphate 431 GT4.hmm - 100 0.00E+00 100 WP_012851072 glycosyltransferase glycosyltransferase family 1 452 GT4.hmm - 100 0.00E+00 100 WP_012853975 protein 13

glycosyltransferase family 1 404 GT4.hmm - 100 0.00E+00 100 WP_012850472 protein 290 GT14.hmm - glycosyl transferase family 14 100 0.00E+00 100 WP_012851388 α,α-trehalose-phosphate synthase [UDP- 470 GT20.hmm trehalose-6-phosphate synthase 100 0.00E+00 99 WP_083789822 forming] (EC 2.4.1.15) 595 GT39.hmm - hypothetical protein 100 0.00E+00 100 WP_012851347 773 GT51.hmm - glycosyl transferase family 51 100 0.00E+00 100 WP_012851396 265 GT81.hmm - glycosyl transferase family 2 100 0.00E+00 100 WP_012850508 504 GT83.hmm - hypothetical protein 100 0.00E+00 100 WP_012854498 DUF2029 domain-containing 429 GT87.hmm - 100 0.00E+00 100 WP_012850510 protein glycosyltransferase family 1 404 GT94.hmm - 100 0.00E+00 100 WP_012850472 protein 327 PL1.hmm pectate lyase (EC 4.2.2.2) pectate lyase 100 0.00E+00 100 WP_012852783 616 PL22_1.hmm - hypothetical protein 100 0.00E+00 99 WP_086014605 1 The name of the enzyme differs between the CAZy databases and non-redundant NCBI proteins.