US 2005O118624A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2005/0118624 A1 Ma et al. (43) Pub. Date: Jun. 2, 2005

(54) FLUORESCENT PROBES FOR RIBOSOMES Related U.S. Application Data AND METHOD OF USE (60) Provisional application No. 60/508.401, filed on Oct. (75) Inventors: Zhenkun Ma, Dallas, TX (US); Jing 3, 2003. Li, Dallas, TX (US); In Ho Kim, Irving, TX (US); Yafei Jin, Dallas, TX Publication Classification (US); Anthony Simon Lynch, Dallas, TX (US); Eric Roche, Carrollton, TX (51) Int. Cl...... C12O 1/68; CO7H 17/08; (US); Doug Beeman, Dallas, TX (US) CO7H 21/04 (52) U.S. Cl...... 435/6; 536/24.1; 536/25.32; Correspondence Address: 544/229; 544/230; 536/7.1 JACKSON WALKER LLP SSNETH CENTRAL EXPRESSWAY (57) ABSTRACT RICHARDSON, TX 75080 (US) Fluorescent probes that have binding- affinity to ribosomes. (73) Assignee: Cumbre Inc., Dallas, TX The fluorescent probes are useful tools for identifying small molecules that bind to the 50S or 30S subunits of the (21) Appl. No.: 10/954,996 bacterial and other ribosomes and Serve as novel ribosome inhibitors. These probes are also useful for determining the (22) Filed: Sep. 30, 2004 interactions between a specific ligand and the ribosome. Patent Application Publication Jun. 2, 2005 Sheet 1 of 39 US 2005/0118624 A1 1. D

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