Flow Cytometric Determination of Genome Size in the Taxodiaceae, Cupressaceae Sensu Stricto and Sciadopityaceae
C2001 The Japan Mendel Society Cytologia 66: 307-311, 2001 Flow Cytometric Determination of Genome Size in the Taxodiaceae, Cupressaceae sensu stricto and Sciadopityaceae Masahiro Hizume1,*, Teiji Kondo2, Fukashi Shibata1 and Ryoko Ishizuka3 1Faculty of Education, Ehime University, Matsuyama 790-8577, Japan 2Forest Tree Breeding Center, Juo, Ibaraki 319-1301, Japan 3Tsukuba Technical Center, Ikeda Science Co., Tsukuba 305-0062, Japan Accepted August 3, 2001 Summary The genome sizes of 13 species of Taxodiaceae, 19 species of Cupressaceae s.s. and Sciadopitys verticillata were determined by flow cytometry of isolated nuclei stained with propidium iodide, using Hordeum vulgare nuclei as an internal standard. In Taxodiaceae, the genomes of Cun- ninghamia lanceolata (28.34 pg/2C) and Taiwania species (25.78, 26.80 pg/2C) were larger than those of other genera/species, which ranged from 19.85 to 22.87 pg/2C. In Cupressaceae s.s., genome size ranged from 20.03 to 27.93 pg/2C among 16 species. The Calocedrus species and Thu- jopsis had a larger genome than most other species. Sciadopitys verticillata had a large genome of 41.60 pg/2C. After comparing the diversity in genome size with previously reported cladograms con- structed using nucleotide sequence data, the tendency of changes in genome size with phylogenetic differentiation is discussed. Key words Cupressaceae, Flow cytometry, Genome size, Taxodiaceae, Sciadopitys. All genera of Taxodiaceae, except for Athrotaxis, are distributed in north-temperate to subtrop- ical regions of the North Hemisphere and most genera are growing in mountainous areas around the Pacific Ocean. Glyptostrobus, Metasequoia, Sequoia, and Sequoiadendron are monotypic genera, and the other genera include only a few species.
[Show full text]