First Detection of Oshv-1 in the Cephalopod Octopus Vulgaris. Is the Octopus a Dead-End for Oshv-1?
Journal of Invertebrate Pathology 183 (2021) 107553 Contents lists available at ScienceDirect Journal of Invertebrate Pathology journal homepage: www.elsevier.com/locate/jip First detection of OsHV-1 in the cephalopod Octopus vulgaris. Is the octopus a dead-end for OsHV-1? Maria Prado-Alvarez a,*, Pablo García-Fernandez´ a, Nicole Faury b, Carlos Azevedo c,d, Benjamin Morga b, Camino Gestal a,* a Marine Molecular Pathobiology Group, Marine Research Institute, Spanish National Research Council, Eduardo Cabello 6, 36208 Vigo, Spain b IFREMER, Laboratoire de G´en´etique et Pathologie des Mollusques Marins, Avenue de Mus de Loup, 17390 La Tremblade, France c Interdisciplinary Center of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal d Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal ARTICLE INFO ABSTRACT Keywords: The ostreid herpes virus (OsHV-1), associated with massive mortalities in the bivalve Crassostrea gigas, was Octopus vulgaris detected for the firsttime in the cephalopod Octopus vulgaris. Wild adult animals from a natural breeding area in Crassostrea gigas Spain showed an overall prevalence of detection of 87.5% between 2010 and 2015 suggesting an environmental OsHV-1 source of viral material uptake. Overall positive PCR detections were significantly higher in adult animals (p = Gene expression 0.031) compared to newly hatched paralarvae (62%). Prevalence in embryos reached 65%. Sequencing of In situ hybridization positive amplicons revealed a match with the variant OsHV-1 µVar showing the genomic features that distinguish this variant in the ORF4. Gill tissues from adult animals were also processed for in situ hybridization and revealed positive labelling.
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