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Development of Octopus Aquaculture Final Report FRDC Project No 2009/206 Fisheries Research Report No. 262, 2015 Development of octopus aquaculture Final Report FRDC Project No 2009/206 S. Kolkovski, J. King, N. Watts, M. Natale, A. Mori, R. Cammilleri, C. Cammilleri Fisheries Research Division Western Australian Fisheries and Marine Research Laboratories PO Box 20 NORTH BEACH, Western Australia 6920 4204/14 Correct citation: Kolkovski, S., King, J., Watts, N., Natale, M.,Mori, A., Cammilleri, R., Cammilleri, C. Development of octopus aquaculture Final Report. FRDC Project No. 2009/206. Fisheries Research Report No. 262. Department of Fisheries, Western Australia. 144pp. Researcher Contact Details FRDC Contact Details Name: Sagiv Kolkovski Address: 25 Geils Court, Deakin ACT 2600 Address: 39 Northside Drive, Hillarys 6029 WA Phone: 02 6285 0400 Phone: 08 9203 0220, 0417 940 498 Fax: 02 6285 0499 Fax: 08 9203 0199 Email: [email protected] Email: [email protected] Web: www.frdc.com.au In submitting this report, the researcher has agreed to FRDC publishing this material in its edited form. Ownership of Intellectual property rights Unless otherwise noted, copyright (and any other intellectual property rights, if any) in this publication is owned by the Fisheries Research and Development Corporation, Department of Fisheries Western Australia. This publication (and any information sourced from it) should be attributed to [Kolkovski, S., Department of Fisheries, Western Australia, 2015, Development of octopus aquaculture, Perth, Western Australia, February.] Creative Commons licence All material in this publication is licensed under a Creative Commons Attribution 3.0 Australia Licence, save for content supplied by third parties, logos and the Commonwealth Coat of Arms. Creative Commons Attribution 3.0 Australia Licence is a standard form licence agreement that allows you to copy, distribute, transmit and adapt this publication provided you attribute the work. A summary of the licence terms is available from creativecommons.org/licenses/by/3.0/au/deed.en. The full licence terms are available from creativecommons.org/licenses/by/3.0/au/legalcode. Inquiries regarding the licence and any use of this document should be sent to: [email protected] Disclaimer The authors do not warrant that the information in this document is free from errors or omissions. The authors do not accept any form of liability, be it contractual, tortious, or otherwise, for the contents of this document or for any consequences arising from its use or any reliance placed upon it. The information, opinions and advice contained in this document may not relate, or be relevant, to a reader’s particular circumstances. Opinions expressed by the authors are the individual opinions expressed by those persons and are not necessarily those of the publisher, research provider or the FRDC. The Fisheries Research and Development Corporation plans, invests in and manages fisheries research and development throughout Australia. It is a statutory authority within the portfolio of the federal Minister for Agriculture, Fisheries and Forestry, jointly funded by the Australian Government and the fishing industry. © Fisheries Research and Development Corporation and Department of Fisheries Western Australia. July 2015. All rights reserved. ISSN: 1035 - 4549 ISBN: 978-1-921845-84-0 ii Fisheries Research Report [Western Australia] No. 262, 2015 Contents 1.0 Executive summary ..................................................................................................... 1 2.0 Background ................................................................................................................... 3 3.0 Objectives ...................................................................................................................... 6 4.0 Larvae rearing .............................................................................................................. 6 4.1 Systems ................................................................................................................... 6 4.1.1 40 lt system .................................................................................................. 6 4.1.1.1 Tanks .............................................................................................. 6 4.1.1.2 Tank components ........................................................................... 6 4.1.1.3 Tank environment .......................................................................... 7 4.1.2 270 lt system ................................................................................................ 8 4.1.2.1 Tanks .............................................................................................. 8 4.1.2.2 Tank components ........................................................................... 8 4.1.2.3 Tank environment .......................................................................... 9 4.1.3 1000 lt system .............................................................................................. 10 4.1.3.1 Tanks .............................................................................................. 10 4.1.3.2 Tank components ........................................................................... 10 4.1.3.3 Tank environment .......................................................................... 11 4.1.4 Tank volume ................................................................................................ 11 4.1.4.1 Introduction .................................................................................. 11 4.1.4.2 Methods ........................................................................................ 12 4.1.4.3 Discussion ...................................................................................... 15 4.2 Nutrition .................................................................................................................. 16 4.2.1 Amino acid treatment ................................................................................... 16 4.2.1.1 Introduction ................................................................................... 16 4.2.1.2 Methods ........................................................................................ 17 4.2.1.3 Results .......................................................................................... 18 4.2.1.4 Discussion ..................................................................................... 18 4.2.2 Live feed treatment....................................................................................... 19 4.2.2.1 Introduction ................................................................................... 19 4.2.2.2 Methods ........................................................................................ 19 4.2.2.3 Results ........................................................................................... 20 4.2.2.4 Discussion ...................................................................................... 21 4.3 Environment .......................................................................................................... 24 4.3.1 Live Chlorella .............................................................................................. 24 4.3.1.1 Introduction ................................................................................... 24 4.3.1.2 Methods ........................................................................................ 24 4.3.1.3 Results .......................................................................................... 25 4.3.1.4 Discussion ...................................................................................... 26 4.3.2 Temperature ................................................................................................. 26 4.3.2.1 Introduction .................................................................................. 26 4.3.2.2 Methods ........................................................................................ 26 4.3.2.3 Results ........................................................................................... 27 4.3.2.4 Discussion ...................................................................................... 28 Fisheries Research Report [Western Australia] No. 262, 2015 iii 4.4 Bacteria treatment .................................................................................................. 29 4.4.1 Formalin treatment ...................................................................................... 29 4.4.1.1 Introduction ................................................................................... 29 4.4.1.2 Methods ......................................................................................... 29 4.4.1.3 Results ........................................................................................... 33 4.4.2 Formalin treatment 2 .................................................................................... 36 4.4.2.1 Introduction ................................................................................... 36 4.4.2.2 Methods ......................................................................................... 36 4.4.2.3 Results ........................................................................................... 39 4.4.3 Formalin treatment 3 .................................................................................... 40 4.4.3.1 Introduction ................................................................................... 40 4.4.3.2 Methods ........................................................................................
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