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©2016 ASP Ins., Afarand Scholarly Publishing Institute, Iran ISSN: 2252-0805 Quarterly of the Horizon of Medical Sciences 2016;22(3):229-235 Comparison the Protective Effects of L- and Acetyl L-Carnitine on Blood and Peroxidation Level in Diabetic Rats

A R T I C L E I N F O A B S T R A C T Aims Article Type New medications with less side-effect are increasingly noticed now a day. L-Carnitine and Acetyl L-Carnitine reduce the secondary side-effects of Type I . The aim of this Original Research Authors Materialsstudy was & to Methods investigate the effects of oral administration of the materials on the blood PhD, glucose and the lipid per-oxidation of the liver and brain tissues in the diabetic rats. 1 DVM, In the experimental study, 50 male Wistar rats were studied. The rats 1 Hajinezhad M.R.*DVM, were randomly divided into five groups including control (the healthy rats), negative control Hajian Sh. 2 PhD, Saghayei S.3 PhD (the diabetic rats), and three treatment diabetic groups. The diabetic groups received 110mg/ Samzadeh-Kermani A.R. Kg alloxan via injection to become diabetic. The treatment groups received L-Carnitine, Acetyl Nabavi R. L-Carnetine, and L-Carnetine with Acetyl L-Carnetine (300mg/Kg) as gavage for 30 days. The How to cite this article lipid per-oxidation, the serum glucose, the lipid profile, and the liver were measured Findingsat the end of the experiment. Data was analyzed using one-way ANOVA followed by Tukey Hajinezhad M.R, Hajian Sh, complementary test. Saghayei S, Samzadeh-Kermani The blood concentration, , cholesterol, creatinine, the serum A.R, Nabavi R. Comparison the liver enzymes, and the level of the liver tissue malondialdehyde significantly decreased Protective Effects of L-Carnitine in treatment diabetic group than diabetic group without any treatment, while HDL level and Acetyl L-Carnitine on Blood Glucose and Lipid Peroxidation increased as well (p<0.05). The brain tissue malondialdehyde and the serum HDL decreased Level in Diabetic Rats. Quarterly and increased due to the administration of Acetyl L-Carnitine, respectively. Nevertheless, it of the Horizon of Medical Sciences. Conclusionaffected no other parameter significantly. The positive effects of L-Carnitine were reduced by 2016;22(3):229-235. the administration of Acetyl L-Carnitin with L-Carnitine. The administration of L-Carnitine further reduces the secondary side-effects of diabetes than Acetyl L-Carnitine. In addition, simultaneous administration of the materials isKeywords not recommended.

Diabetes Mellitus; L-Carnitine; Acetyl L-Carnitine; Rats C I T A T I O N L I N K S [1] [2] [3] Preserved β-cell[4] function in type 1 diabetes by ... Modulation of tissue fatty acids by L-carnitine[5] attenuates ... The effect of two-week l-carnitine[6] supplementation on *Basic Science Department, Veteri- exercise-induced ... L-Carnitine treatment for congestive : Experimental 1 [7] nary Medicine Faculty, University and ... L-carnitine supplementation for the treatment of ... A review on the role of Zabol, Zabol, Iran [8] Basic Science Department, Veteri- of in the management of ... Toward a biomarker[9] of oxidative stress: A nary2 Medicine Faculty, University fluorescent probe for exogenous and ... Comparison of E, L-carnitine and of Zabol, Zabol, Iran in ameliorating carbon tetrachloride and ... In vivo effect of acetyl-L- Chemistry Department, Basic Sci- [10] ence3 Faculty, University of Zabol, carnitine on succinate oxidation, adenine nucleotide pool and lipid composition[11] of synaptic Zabol, Iran and non-synaptic mitochondria from ... Protective effect of hydro[12] alcoholic extract Pathobiology Department, Veteri- from Prosopisfarcta leaves on lipid peroxidation of serum and ... Preventive effect nary Faculty, University of Zabol, [13] Zabol, Iran of berberisintegerrima on the serum levels of glucose and [14] in ... Assay for lipid Correspondence peroxides in animal tissues by ... Effect of hydroalcoholic extract of Prosopisfarcta Address [15] pod on liver histopathology and malondialdehyde level in ... L-carnitine[16] is essential : Basic Science Department, to beta-oxidation of quarried from ... Therapeutic effect of L-carnitine on Veterinary Medicine Faculty, Univer- sialic acid, soluble Fas (sFas) and other biochemical variables in ... Comparison of the sity of Zabol, Bonjar Ave, Zabol, Iran. [17] Phone +985422323567 effects of L-carnitine and acetyl-L-carnitine on carnitine levels, ambulatory activity, and FaxPostal Code: 98613-35856 [18] [19] : ... Levels of carnitines in[20] brain and other tissues of rats of different ages: effect of ... : +985422323567 [21] Effects of short-term l-arginine supplementation on lipid profile and ... [22] Protective [email protected] effect of L-carnitine and ... L-Carnitine changes the levels of insulin-like growth ... Article History [23] Effects of L‐carnitine[24] treatment on oxidant/[25] state and ... Acetyl-L- Carnitine and nicotinamide for[26] prevention of ... L-carnitine inhibits hypoglycemia- Received: October 24, 2015 induced brain ... Acetyl-L-carnitine enhances ... Acetyl-L-carnitine and lipoic Accepted: May 10, 2016 acid improve mitochondrial ... Protective effects of L-carnitine on myoglobinuric acute ... Copyright©ePublished: June2016 30, ASP 2016 Ins. This open-access article is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits Share (copy and redistribute the material in any medium or format) and Adapt (remix, transform, and build upon the material) under the Attribution-NonCommercial terms. // :'0+ 1+% // :-2 1+% * [email protected] :&34 54 * . PhD !" #$ %& ! ' () * DVM /& 0 12$ 3(!- 4!5 +,- [1] :' 89 $ ' #7 6- . - DVM 6- !$ .- ;!)7 !$ !$ . -! : <9 ,-= >- !5 PhD ! ?'- 6 !5 !- ( ,-= ! )$ #" ,$!- @5 A0 B! PhD "# . "#$!% F2& (D" E 6- .- C=

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= @> - > `(= (- 5 ,E 1!$ @! 6 !$ 6- 2 v$R U ,$!- ?= !$ + )B- ) !$ - :$ " .[23] 67 !$ ,D- Z !$ ?: 6- - + rV!U . #$ (C, 6 67 c- " `(= (- - 1\U C= <9 4!5 ;7 6Y! A[' #$ ! 67 !$ k,D- Z !$ @! . !> 2BBK ,- - D(G- )$ %! ) !$ @! $5 + #B <9 - !$ 6 67 :$ " 6- 9O\ H ,M . #$ (= + - ( ,$!- A0 B! !$ 6 aN>- !@ ,!,& 6B -9 W,> - - H 1!$ $5 + . F !$ @! )$!= :T9 ]JU H . [16] W! $ c- " `(= (- - !$ @! . 6 2:H <:E . ) [17] jBK W! ) 6 > #P& 5 . c- 5 = 7<' 4I9 " ?= #$ !$ H 7 ^C- )$!= Q ,9 - 6- $ "> " 2D(G- <:E 2BBK 1d9 649 . R( @"& !$ :t- 2H 6,> ^= - A0 :$ 4!:- 2 !$ @! !$ 6 67 :$ ^= - 1 W! 2J 0 Z :$ [20, <:E 2D(G- W! 6,,$ $ ,D- #$ . w- !$ @! + - 6 D(G- - !$ @! !$ $ 7 .21] + 6 6,= 2D(G- - C,>& , ,$ - ,D- #" 1 6$ " 6,,$Y>& H ,M #" !$ @! . ' - ! 67 :) :$ ^= . !$ @! !$ - + - L W! ' !$ EJO@5 + >& 2D(G- . Q)$ H !$ @! 3 ,$ - 2- 37<$ 37X<- 3 HDL !$ @! ! 67 1\U 1 #$ - U <$ U 4!:- 2 #" HDL )$& #$ . #" ,D- Z !,$ 67 K 67 ,$ - $ Z .[22] H @( - R(/&R( (D" $5 + \U D(G- .[26] ,D- S4!5 !$ + 6 > 2D(G- - ! 67 :) ,$ - !$ KO ;- )<7R c- ?= - -2- ,M #" - L . #$ !J Q 2- KO ;- Q . #$ !-& :C !$ :t- H #-= A= !J 2- dR ,!"7 E () K - C,>& , .,$- B R( )$& > W! . "\ !$ % C= -= ,M . 6J! !$ V 6,= !" #$ I" L"U :C ?: !$ + !$ + -2- D - C,>& 3 H $ ,0 1 Q)<7R 2 G\ ,M . Q)<7R ;- -u- c-

Q Horizon Med Sci Vol. 22, Iss. 3, Sum 2016 diabetes and its complications. Biomed Pharmacother. <7 <7 - #" !$ @! 2005;59(7):365-73. 7- Chen J, Zeng L, Xia T, Li S, Yan T, Wu S, et al. Toward a . 76 biomarker of oxidative stress: A fluorescent probe for (D" 39 - D(G- K- exogenous and endogenous malondialdehyde in living cells. Anal Chem. 2015;87(16):8052-6. V$ 1))$& ,- :$ )$!= ^= 8- Shaker ME, Houssen M, Abo-Hashem EM, Ibrahim TM. Comparison of , L-carnitine and melatonin in >- nZ - C,>& .$ 6 )$&<7 ameliorating carbon tetrachloride and diabetes induced " 3 )$ %! _:- a5 1= hepatic oxidative stress. J Physiol Biochem. 2009;65(3):225-33. . c- :$ 9- Petruzzella V, Baggetto LG, Penin F, Cafagna F, Ruggiero FM, Cantatore P, et al. In vivo effect of acetyl-L- carnitine on succinate oxidation, adenine nucleotide pool and lipid composition of synaptic and non-synaptic mitochondria from cerebral hemispheres of senescent 89 #$ !$ @! )B- !$ + rats. 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