Cucurbitaceae 2012 Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae October 15-18, 2012, Antalya-Turkey

Total Page:16

File Type:pdf, Size:1020Kb

Cucurbitaceae 2012 Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae October 15-18, 2012, Antalya-Turkey Cucurbitaceae 2012 Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae October 15-18, 2012, Antalya-Turkey www.cucurbitaceae2012.org 1 2 EUROPEAN ASSOCIATION FOR RESEARCH ON PLANT BREEDING EUROPÄISCHE GESELLSCHAFT FÜR ZÜCHTUNGSFORSCHUNG EUCARPIA ASSOCIATION EUROPÉENNE POUR L’AMÉLIORATION DES PLANTES Cucurbitaceae 2012 Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae October 15-18th, 2012, Antalya - TURKEY Editors N. Sari I. Solmaz V. Aras Publisher: Çukurova University 3 Photograph on the front cover: N. Sari Organizing institution: Çukurova University, Faculty of Agriculture Department of Horticulture Adana, Turkey ISBN : 978-605-63297-0-8 Printed By : Bilkon Tourism & Travel Agency 4 President Prof. Dr. Nebahat Sari Çukurova University Honorary Presidents Prof. Dr. Kazım Abak Lefke European University Assoc. Prof. Dr. Masum Burak Director, Ministry of Food, Agriculture and Livestock Secretary Dr. İlknur Solmaz Çukurova University Scientific Committee Angela Davis Harris Moran Seed Company (USA) Maria J. Diez Niclos Universidad Politécnica de Valencia (Spain) Catherine Dogimont Institut National de la Recherche Agronomique (France) Hiroshi Ezura Tsukuba University (Japan) Xingfang Gu Chinese Academy of Agricultural Science (China) Jordi Garcia Mas Institut de Recerca i Tecnologia Agroalimentàries (Spain) Nurit Katzir Agricultural Research Organization (Israel) Elzbieta U. Kozik Research Institute of Vegetable Crops (Poland) Aleš Lebeda Palacky University (Czech Republic) Amnon Levi United States Dept. of Agriculture-Agricultural Research Service (USA) Harry Paris Agricultural Research Organization (Israel) Maria Belén Picó Universidad Politécnica de Valencia (Spain) Michel Pitrat Institut National de la Recherche Agronomique (France) Nebahat Sari Çukurova University (Turkey) Yaakov Tadmor Agricultural Research Organization (Israel) Todd Wehner North Carolina State University (USA) Yong Xu National Engineering Research Center for Vegetables (China) Halit Yetişir Erciyes University (Turkey) Xingping Zhang Syngenta (USA) 5 Organizing Committee Members Prof. Dr. Yeşim Yalçın Mendi Çukurova University Prof. Dr. H.Yıldız Daşgan Çukurova University Assoc. Prof. Dr. Yıldız Aka Kaçar Çukurova University Assist. Prof. Dr. Nezihe Köksal Çukurova University Dr. Abdullah Ünlü BATEM, Ministry of Food, Agriculture and Livestock Dr. Suat Yılmaz BATEM, Ministry of Food, Agriculture and Livestock Dr. Filiz Boyacı BATEM, Ministry of Food, Agriculture and Livestock Dr. Rana Kurum BATEM, Ministry of Food, Agriculture and Livestock Dr. Volkan Gözen BATEM, Ministry of Food, Agriculture and Livestock Dr. Asu Oğuz BATEM, Ministry of Food, Agriculture and Livestock MSc. Mine Ünlü BATEM, Ministry of Food, Agriculture and Livestock MSc. Yavuz Köksal BATEM, Ministry of Food, Agriculture and Livestock MSc. Veysel Aras ALATA, Ministry of Food, Agriculture and Livestock Res. Assist. Özhan Şimşek Çukurova University 6 PREFACE On behalf of the Organizing Committee of EUCARPIA Cucurbitaceae 2012, it gives me great pleasure to welcome you in Antalya, Turkey. The first EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae was organized by INRA, France 24 years ago. The following meetings were held in Poland (1992), Spain (1996), Isra- el (2000), Czech Republic (2004) and France (2008). It is a great honor and privilege for us to host the 10th EUCARPIA Cucurbitaceae Meeting in Antalya. Turkey is an important country in agricultural production and very rich in Cucurbit genetic resources. You will have the opportunity to see our genetic diver- sity in all Cucurbit species during our technical excursion. We expect more than 220 participants from 33 countries at the meeting in Rixos Downtown Hotel in Antalya. About 70% of the participants are from public research institutions and 30% are from private seed companies. We are pleased to welcome the international scientific community on cucurbit genetics, breeding and genomics. The proceeding contains 35 full text papers presented in oral sessions and 96 papers presented as posters at the Xth EUCARPIA Meeting on Genetics and Breed- ing of Cucurbitaceae. The authors should be thanked for their willingness to share their results. Our special thanks to scientific committee members for their contribution in evaluating oral and poster presentation and reviewing all the papers. We also thank the keynote speakers for their informative presentations. This meeting is vital for transferring results, stimulating new ideas for re- search topics, and in fostering co-operation possibilities. We would like to thank EUCARPIA, our public (Çukurova University, Ministry of Food, Agriculture and Livestock and Turkish Scientific and Technological Council) and private sponsors (Antalya Tarim, Manier Seed, Yuksel Seed, Syngenta, AG Seed, Fito Seed, Multi Seed, Nunhems and Rijk Zwaan) for accepting financial support of the meeting. We are grateful to the public institutions and private companies taking place in the technical trip. We also thank Bilkon Tourism and Travel Agency for their excellent service and organization. We will do our best to make Cucurbitaceae 2012 a most informative and enjoyable experience for all participants. We hope you enjoy this meeting and your stay in Antalya, Turkey. Antalya, October 2012 Prof. Dr. Nebahat SARI On behalf of the Organizing Committee 7 The organization of this meeting has been possible by the financial support of: Çukurova University EUCARPIA TÜBİTAK Batı Akdeniz Agricultural Research Institute Alata Horticultural Research Station Antalya Tarım Manier Seed Yüksel Seed Syngenta AG Seed Fito Seed Multi Seed Rijk Zwaan Nunhems 8 TABLE OF CONTENTS AUTHORS INDEX……………………………………………………………… 21 ORAL PRESENTATIONS……………………………………………………… 29 Session I: GENETIC RESOURCES AND BREEDING Domestication and diversification of melon………………………………......... 31 M. Pitrat Exploiting genetic diversity in Citrullus spp. to enhance watermelon cultivars……...……………………………….........……………………………... 41 A. Levi, J.A. Thies, W. P. Wechter, C. Kousik, K. Ling, H. Harrison, A. Simmons, U.K. Reddy, P. Nimmakayala, Z. Fei, S. Mitchell, Y. Xu, K. Tadmor and N. Katzir Association mapping of some selected watermelon traits using molecular markers…………………………………………………………………………... 49 O. Gulsen, M. Yagcioglu, H. Yetisir, I. Solmaz and N. Sari Early viability assessment in mutant populations of Cucurbita pepo ………... 50 N. Vicente-Dólera, V. Pinillos, M. Moya, F. Rubira, D. Martínez-Valdivieso, M. Del Río-Celestino, B. Román and P. Gómez Promotion of growth and health of Styrian oil pumpkins (Cucurbita pepo var. styriaca) by bacterial broad-spectrum antagonists……………………….. 56 E. Adam, M. Fürnkranz, H. Müller, M. Grube, H. Huss, J. Winkler and G. Berg Variation for bitterness and other fruit traits in bitter gourd (Momordica charantia L.) collections………………………………......................................... 66 N.P.S. Dhillon and S. Phethin Session II: GENETIC RESOURCES AND BREEDING Mode of inheritance of bitterness and spine colour in cucumber fruits (Cucumis sativus L.) ……………………………….............................................. 70 M. Pitchaimuthu, O.P. Dutta, K.R.M. Swamy and K. Souravi Development of fusarium wilt-resistant F1 hybrids of bottle gourd (Lagenaria siceraria Standl.) for watermelon rootstocks……………………… 74 Y.C. Huh, W.M. Lee, H.C. Ko, D.K. Park, K.S. Park, H.J. Lee, S.G. Lee, and K.D.Ko Undulate leaf blade margin of patty pan (Cucurbita pepo var. patisonina Greb.) new spontaneous mutation………………………………........................ 83 A. Korzeniewska and K. Niemirowicz-Szczytt 9 Stability of fruit yield in watermelon genotypes tested in multiple US environments……………………………….........……………………………….. 84 M. Dia, T.C. Wehner, R. Hassell, D.S. Price, G.E. Boyhan, S. Olson, S. King, A.R. Davis, G.E. Tolla, J. Bernier and B. Juarez Occurrence of androgynous inflorescence in Luffa cylindrica exhibiting dominant monogenic inheritance……………………………….......................... 89 S.P. Singh, A. Kumar and D.K. Dubey Determination and analysis of drought resistance index for watermelon during germination stage ……………………………….........…………………. 98 J Ma, X. Zhang, Y. Zhang, C. Li and Y. Mo Session III: GENOMICS AND FRUIT QUALITY Dynamic characteristics of enzymes and transcriptome related to sugar metabolism and accumulation in sweet and non-sweet watermelon fruits…... 103 Y. Xu, S. Guo, J. Liu, Z. Fei, H. He, H. Zhang, Y. Ren, H. Sun, G. Gong, Y. Zheng, M. Huang and S. Zhong eth3.5 and eth6.3 control climacteric fruit ripening in the near isogenic line SC3-5-1…………………………………………………………………………… 112 J. Vegas, P. Rios, W. Sanseverino, A.J. Monforte and J. Garcia-Mas Effect of carpel primordia-targeted inhibition of ethylene perception on sex expression, fruit set, and fruit ripening in melon (Cucumis melo L.) …….…...116 J.T. Switzenberg, L. Fernando, S.A. Hammar and R. Grumet Enzyme activity and Cucurbitacin content in relation to fusarium wilt resistance in melon…………………………………………………………….….126 E. Madadkhah and M. Lotfi Session IV: PEST AND DISEASE RESISTANCE Fusarium wilt of watermelon: A historical review…………………………….. 136 R.D. Martyn Resistance of melon to Cucumber Vein Yellowing Virus (CVYV) ……..……….157 M. Pitrat, C. Wipf-Scheibel, D. Besombes, C. Desbiez and H. Lecoq Genetic studies on resistance to downy mildew in cucumber…………….……165 E.U. Kozik, U. Klosińska, T.C. Wehner Application of a new approach for characterization and denomination of races of cucurbit powdery mildews–a case study of Czech pathogen populations…………………………………………………………………..……
Recommended publications
  • DETERMINATION of GENETIC DIVERSITY in WATERMELON (Citrullus Lanatus (Thunb.) Matsum & Nakai) GERMPLASMS
    DETERMINATION OF GENETIC DIVERSITY IN WATERMELON (Citrullus lanatus (Thunb.) Matsum & Nakai) GERMPLASMS A Thesis Submitted to the Graduate School of Engineering and Sciences of İzmir Institute of Technology in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE in Molecular Biology and Genetics by Zehra İpek ULUTÜRK December 2009 İZMİR i ii ACKNOWLEDGEMENTS I am heartily thankful to my advisor, Prof. Dr. Anne FRARY, whose encouragement, guidance, excellent advice and support from the initial to the final level of this study enabled me to develop an understanding of the subject. My sincere thanks to my coadvisor Assoc. Prof. Dr. Sami DOĞANLAR for giving me the opportunity to study and supporting me during the completion of the project. I also thank my colleagues of the department of Molecular Biology and Genetics. Discussions and cooperation with all of my colleagues have contributed substantially to this study. Finally, I owe special gratitude to my family for their patience and continuous support of all my undertakings, scholastic and otherwise. iii ABSTRACT DETERMINATION OF GENETIC DIVERSITIY IN WATERMELON (Citrullus lanatus (Thunb.) Matsum & Nakai) GERMPLASMS With 1.4 million tons of production, Turkey ranks second behind China in worldwide production of watermelon. Watermelon is grown at different times throughout the country with highest consumption during the summer and fall. Watermelon is an economically and socio-economically important crop throughout the country and especially in coastal regions where much of the cultivation occurs. In this research, 90 watermelon accessions collected from different regions of the world by the USDA were molecularly characterized using 40 SRAP marker combinations based on the UPGMA (Unweighted Pair Group Method) and Neighbor Joining methods.
    [Show full text]
  • Friday 1 April 2016
    Friday 1st April 2016 (For the period 1st to 5th April 2016) Weblink For District AAS Bulletin: http://www.imdagrimet.gov.in/node/3545 State Composite AAS Bulletin: http://www.imdagrimet.gov.in/node/3544 1 Standardised Precipitation Index Four Weekly for the Period 3rd March to 30th March 2016 Extremely/severely wet conditions experienced in a few districts of Konkan & Goa and Saurashtra, Kutch & Diu; Upper Siang district of Arunachal Pradesh; Rohtas district of Bihar; Allahabad, Fatehpur, Mirzapur, Sonbhadra, Varanasi, Hamirpur districts of Uttar Pradesh; Bhatinda, Muktsar, Faridkot districts of Punjab; Reasi, Pulwama, Ramban districts of Jammu & Kashmir; Churu district of Rajasthan; Chhatarpur, Tikamgarh districts of Madhya Pradesh; Surat, Narmada, Navsari districts of Gujarat Region; Nashik district of Maharashtra. Extremely/Severely dry conditions experienced in Jaintia Hills district of Meghalaya; Dindigul, Salem districts of TamilNadu. Moderately dry conditions experienced in few districts of Arunachal Pradesh; Tamil Nadu; Karbi Anglong, Hailakandi districts of Assam; Mayurbhanj district of Odisha; Seraikela- Khar district of Jharkhand; Ladakh (Leh), district of Jammu & Kashmir; Khammam district of Telangana; Chamarajanagar, Hassan, Shimoga districts of Karnataka; Ernakulam, Malappuram districts of Kerala. Rest of the country experienced moderately wet/ mildly dry/mildly wet conditions. 2 Contour maps for Mean Maximum and Minimum Temperature and their anomaly for the week ending on 30.03.2016 Actual Mean Maximum Temperature (oC) in India Mean Maximum Temperature (oC) Anomaly in for the week ending 30.03.2016 India for the week ending 30.03.2016 Mean maximum temperature was above 400C over Mean maximum temperature was above normal by 4 many parts of Marathwada, North Interior to 60C over many parts of Himachal Pradesh and Karnataka, some parts of Gujarat region, Madhya isolated pockets of Uttarakhand, North Interior Maharashtra, Vidarbha, Telangana, Rayalaseema, Karnataka and West Rajasthan.
    [Show full text]
  • 20640Edfcb19c0bfb828686027c
    FEMS Microbiology Reviews, fuz024, 44, 2020, 1–32 doi: 10.1093/femsre/fuz024 Advance Access Publication Date: 3 October 2019 Review article REVIEW ARTICLE Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogen Xanthomonas Shi-Qi An1, Neha Potnis2,MaxDow3,Frank-Jorg¨ Vorholter¨ 4, Yong-Qiang He5, Anke Becker6, Doron Teper7,YiLi8,9,NianWang7, Leonidas Bleris8,9,10 and Ji-Liang Tang5,* 1National Biofilms Innovation Centre (NBIC), Biological Sciences, University of Southampton, University Road, Southampton SO17 1BJ, UK, 2Department of Entomology and Plant Pathology, Rouse Life Science Building, Auburn University, Auburn AL36849, USA, 3School of Microbiology, Food Science & Technology Building, University College Cork, Cork T12 K8AF, Ireland, 4MVZ Dr. Eberhard & Partner Dortmund, Brauhausstraße 4, Dortmund 44137, Germany, 5State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China, 6Loewe Center for Synthetic Microbiology and Department of Biology, Philipps-Universitat¨ Marburg, Hans-Meerwein-Straße 6, Marburg 35032, Germany, 7Citrus Research and Education Center, Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, 700 Experiment Station Road, Lake Alfred 33850, USA, 8Bioengineering Department, University of Texas at Dallas, 2851 Rutford Ave, Richardson, TX 75080, USA, 9Center for Systems Biology, University of Texas at Dallas, 800 W Campbell Road, Richardson, TX 75080, USA and 10Department of Biological Sciences, University of Texas at Dallas, 800 W Campbell Road, Richardson, TX75080, USA ∗Corresponding author: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China.
    [Show full text]
  • Diversität, Anbau, Verwendung Teil 2: Kürbisgewächse
    Alte und neue Nutzpflanzen: Diversität, Anbau, Verwendung Teil 2: Kürbisgewächse Mormodica charantia (Bittermelone), einer der ursprünglichsten Vertreter der kultivierten Cucurbitoideae. Die sich am Blütenende öffnende Frucht mit den herausfallenden Samen erinnert an die Nhandiroboideae. [WP] Webinar von Dipl.-Biol. Eike Wulfmeyer anstiftung, 14.2.2017 Inhalt • Übersicht Artenporträts • Sicyoeae • Vorkultur • Schwammgurke • Inkagurke • Chayote • Kultur • Benincaseae • Mexikanisches Minigürkchen • Pflanzenschutz • Horngurke • Wassermelone • Kalebasse • Bestäubung • Indischer Apfelkürbis • Cucurbiteae • Saatgutgewinnung • Feigenblattkürbis • Trompetenzucchini Übersicht I – Kürbisgewächse (Cucurbitaceae) • 800 bis knapp 1000 Arten in rund 100 Gattungen • Nhandiroboideae (Zanonienähnliche) und Cucurbitoideae (Kürbisähnliche) • Weitgehend auf frostfreie Gebiete beschränkt, in Mitteleuropa nur Zaunrübe (Bryonia) heimisch • Typischerweise Fruchtgemüse, aber viele †Cucurbitaciphyllum Arten auch anderweitig genutzt (vor allem lobatum, Blatt [M14] Samen, bei einigen Blätter und Sprosse) • Meist einjährige Kletterpflanzen mit gelappten Blättern, hohlen Sprossen, und spiraligen Ranken • 5zählige Blüten • Panzerbeeren (Pepos) Cucurbita pepo, männliche Blüten • Oft signifikanter Gehalt an giftigen Bitterstoffen [WP] in den meisten Pflanzenteilen • Insektenbestäubung (Bienen, Hummeln, Käfer) • Dunkelkeimer, Keimtemperatur hoch • Samen meist sehr lange lebensfähig (bis zu 10 Jahren und mehr) • In der Regel extreme Starkzehrer Cucumis sativus, unreife
    [Show full text]
  • Genetic Resources of the Genus Cucumis and Their Morphological Description (English-Czech Version)
    Genetic resources of the genus Cucumis and their morphological description (English-Czech version) E. KŘÍSTKOVÁ1, A. LEBEDA2, V. VINTER2, O. BLAHOUŠEK3 1Research Institute of Crop Production, Praha-Ruzyně, Division of Genetics and Plant Breeding, Department of Gene Bank, Workplace Olomouc, Olomouc-Holice, Czech Republic 2Palacký University, Faculty of Science, Department of Botany, Olomouc-Holice, Czech Republic 3Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany Academy of Sciences of the Czech Republic, Olomouc-Holice, Czech Republic ABSTRACT: Czech collections of Cucumis spp. genetic resources includes 895 accessions of cultivated C. sativus and C. melo species and 89 accessions of wild species. Knowledge of their morphological and biological features and a correct taxonomical ranging serve a base for successful use of germplasm in modern breeding. List of morphological descriptors consists of 65 descriptors and 20 of them are elucidated by figures. It provides a tool for Cucumis species determination and characterization and for a discrimination of an infraspecific variation. Obtained data can be used for description of genetic resources and also for research purposes. Keywords: Cucurbitaceae; cucumber; melon; germplasm; data; descriptors; infraspecific variation; Cucumis spp.; wild Cucumis species Collections of Cucumis genetic resources include pollen grains and ovules, there are clear relation of this not only cultivated species C. sativus (cucumbers) taxon with the order Passiflorales (NOVÁK 1961). Based and C. melo (melons) but also wild Cucumis species. on latest knowledge of cytology, cytogenetics, phyto- Knowledge of their morphological and biological fea- chemistry and molecular genetics (PERL-TREVES et al. tures and a correct taxonomical ranging serve a base for 1985; RAAMSDONK et al.
    [Show full text]
  • Veggies 2018
    Jenny’s Edibles & Blooms VEGGIES [email protected] 2018 Dragon Langerie Bush Bean Maxibel Bush Beans Unique purple stripes mottled over creamy yellow A slender, elegant bean often seen in European- 6-8 inch long flat pods. A delicious conversation style restaurants. They are mouth-watering and piece with super flavor & crispness. Commonly unsurpassed in flavor. Many filets available eaten both as a snap bean and a shell bean. from seed are miniatures, but these dark green stringless beans are a full-sized, 7-inch pod. Maxibel arises on tall, erect plants that require no trellising, and harvest easily. They mature faster than pole beans yielding a huge and concentrated harvest. Romano Bush Bean Fortex Pole Bean This bush-type Romano is the classic flat bean An extraordinary stringless French filet bean with the robust, distinctive full flavor and very growing to over 10 inches long. The delicious heavy yields you would expect from a Romano. round pods have a pronounced nutty bean flavor The plants are compact, and the yield is not only that can be harvested at any size, small and abundant, but starts early, extends over many slender or large and plump. The yields are weeks and will continue to bear until frost. Beans impressive and long lasting. A vigorous climber. are 6 inches long, excellent fresh, and lend themselves equally well to canning and freezing. Romano Pole Bean Golden Grex Beet A personal favorite! This full flavored pole bean is This jewel is a gift to the beet world from breeder early to set pods, and once set, yields a Alan Kapular! Grex, a term from orchid breeding, continuous and glorious harvest of sweet, nutty, alludes to ongoing variation in the gene pool.
    [Show full text]
  • Hsian.G Lectqres on Chinese.Poet
    Hsian.g LectQres on Chinese.Poet: Centre for East Asian Research . McGill University Hsiang Lectures on Chinese Poetry Volume 7, 2015 Grace S. Fong Editor Chris Byrne Editorial Assistant Centre for East Asian Research McGill University Copyright © 2015 by Centre for East Asian Research, McGill University 688 Sherbrooke Street West McGill University Montreal, Quebec, Canada H3A 3R1 Calligraphy by: Han Zhenhu For additional copies please send request to: Hsiang Lectures on Chinese Poetry Centre for East Asian Research McGill University 688 Sherbrooke Street West Montreal, Quebec Canada H3A 3R1 A contribution of $5 towards postage and handling will be appreciated. This volume is printed on acid-free paper. Endowed by Professor Paul Stanislaus Hsiang (1915-2000) Contents Editor’s Note vii Nostalgia and Resistance: Gender and the Poetry of 1 Chen Yinke Wai-yee Li History as Leisure Reading for Ming-Qing Women Poets 27 Clara Wing-chung Ho Gold Mountain Dreams: Classical-Style Poetry from 65 San Francisco Chinatown Lap Lam Classical Poetry, Photography, and the Social Life of 107 Emotions in 1910s China Shengqing Wu Editor’s Note For many reasons, Volume 7 has taken much longer than anticipated to appear. One was the decision to wait in order to have four Hsiang Lectures in this volume rather than the customary three. The delay and small increase in the number of lectures bring new horizons in research on Chinese poetry to our readers. Indeed, in terms of the time frame covered, this volume focuses mostly, but not exclusively, on the twentieth century. Three of the four lectures are fascinating studies of the manifold significations of classical verse and their continued vitality in the discursive space of Chinese politics and culture in a century of modernization.
    [Show full text]
  • J. Bio. & Env. Sci
    J. Bio. Env. Sci. 2016 Journal of Biodiversity and Environmental Sciences (JBES) ISSN: 2220-6663 (Print) 2222-3045 (Online) Vol. 8, No. 2, p. 166-173, 2016 http://www.innspub.net RESEARCH PAPER OPEN ACCESS Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji Rhimi Awatef1*, Boussaid Mohamed2 1Laboratory of Plant Biotechnology, National Gene Bank of Tunisia (NGBT). Boulevard of the Leader Yasser Arafat, ZI Charguia 1, 1080 Tunis Cedex, Tunisia 2Department of Biology, Laboratory of Plant Biotechnology. National Institute of Applied Sciences and Technologies (INSAT). Centre Urbain Nord, B.P. 676, 1080 Tunis Cedex, Tunisia Article published on February 28, 2016 Key words: Buds; caulogenesis; Cucumis melo L. cv. Maazoun and Beji; plant regeneration; tissue culture. Abstract A procedure for the regeneration of melon (Cucumis melo L.) cv. Maazoun and Beji via shoot organogenesis from cotyledon explants is described. Cotyledons explants cultivated on the basal Murashige and Skoog (1962) medium, added with combinations of BAP, NAA and 2,4-D developed adventitious shoots after two weeks. The rates of caulogenesis varied according to the medium hormonal composition and the genotype. The best induction medium for a morphogenic response was MS containing 0.5 mgl-1 NAA and 2 mgl-1 BAP. The highest caulogenesis percentages were 79.16% and 58.33% for Maazoun and Beji cultivars respectively. The mean number of buds per callus was 2.92. Development phases were characterized by histological studies. Some phases of shoot organogenesis were observed in Cucumis melo L. Maazoun and Beji cultivars. The shoots were rooted in MS medium without growth regulators or added NAA (0.5 or 1 mgl-1).
    [Show full text]
  • Histological Study of Organogenesis in Cucumis Melo L. After Genetic Transformation: Why Is It Difficult to Obtain Transgenic Plants? V Chovelon, V
    Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants? V Chovelon, V. Restier, N. Giovinazzo, Catherine Dogimont, J. Aarouf To cite this version: V Chovelon, V. Restier, N. Giovinazzo, Catherine Dogimont, J. Aarouf. Histological study of organo- genesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?. Plant Cell Reports, Springer Verlag, 2011, 30 (11), pp.2001-2011. 10.1007/s00299-011-1108-9. hal- 01332269 HAL Id: hal-01332269 https://hal.archives-ouvertes.fr/hal-01332269 Submitted on 29 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial| 4.0 International License Version définitive du manuscrit publié dans / Final version of the manuscript published in : Plant Cell Reports, 2011, vol 30 (11) :2001-2011 DOI: 10.1007/s00299-011-1108-9 Histological Study of Organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants? V. Chovelon .V. Restier . N. Giovinazzo . C. Dogimont J. Aarrouf INRA Avignon, UR1052, Unité de Génétique et d’Amélioration des Fruits et Légumes, BP 94, 84143 Montfavet Cedex, France e-mail : [email protected] J.
    [Show full text]
  • Genetic Stability in Melon Transformation System: Assessment by Flow Cytometry and ISSR Markers
    Genetic Stability in Melon Transformation System: Assessment by Flow Cytometry and ISSR Markers Mohammad Reza Raji ( [email protected] ) Lorestan University Mostafa Farajpour Crop and Horticultural Science Research Department Research Article Keywords: Gene Transformation, Direct Organogenesis, Genetic Fidelity, Inter Single Sequence Repeat Marker, Flow Cytometry Posted Date: August 17th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-756109/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/14 Abstract Genetic instability in melon species sometimes occurs as a result of in vitro tissue culture and transformation systems. This study describes a new regeneration technique for agrobacterium-mediated co-culture of muskmelon explants (Cucumis melo L. c.v. ‘Khatooni’). Here, no genetic instability was observed in positive PCR regenerants. 4-day-old cotyledonary explants had been infected with LBA4404 Agrobacterium suspensions. The co-cultivation occurred in the presence of 100mg/l rifampicin and 50mg/l kanamycin. The bacteria contained a binary vector pBI121 carrying nopaline synthase by the promoter-neomycin phosphotransferase gene. The regeneration succeeded 65% in selective MS medium containing N6-benzylaminopurine (600 µg/l), β-naphthoxyacetic acid (25 µg/l) and 50mg/l kanamycin in inoculated 4-day-old cotyledonary explants. According to the polymerase chain reaction analysis of neomycin phosphotransferase II gene, transformation was merely successful (8.4%), indicating a substantial miss on a large number of regenerated plants in the selective medium, as a consequence of PGR and antibiotic imbalances. Inter Single Sequence Repeat markers and ow cytometry analyses were used for evaluating the genetic stability and ploidy level of transplants, respectively.
    [Show full text]
  • Phylogenetic Relationships in the Order Cucurbitales and a New Classification of the Gourd Family (Cucurbitaceae)
    Schaefer & Renner • Phylogenetic relationships in Cucurbitales TAXON 60 (1) • February 2011: 122–138 TAXONOMY Phylogenetic relationships in the order Cucurbitales and a new classification of the gourd family (Cucurbitaceae) Hanno Schaefer1 & Susanne S. Renner2 1 Harvard University, Department of Organismic and Evolutionary Biology, 22 Divinity Avenue, Cambridge, Massachusetts 02138, U.S.A. 2 University of Munich (LMU), Systematic Botany and Mycology, Menzinger Str. 67, 80638 Munich, Germany Author for correspondence: Hanno Schaefer, [email protected] Abstract We analysed phylogenetic relationships in the order Cucurbitales using 14 DNA regions from the three plant genomes: the mitochondrial nad1 b/c intron and matR gene, the nuclear ribosomal 18S, ITS1-5.8S-ITS2, and 28S genes, and the plastid rbcL, matK, ndhF, atpB, trnL, trnL-trnF, rpl20-rps12, trnS-trnG and trnH-psbA genes, spacers, and introns. The dataset includes 664 ingroup species, representating all but two genera and over 25% of the ca. 2600 species in the order. Maximum likelihood analyses yielded mostly congruent topologies for the datasets from the three genomes. Relationships among the eight families of Cucurbitales were: (Apodanthaceae, Anisophylleaceae, (Cucurbitaceae, ((Coriariaceae, Corynocarpaceae), (Tetramelaceae, (Datiscaceae, Begoniaceae))))). Based on these molecular data and morphological data from the literature, we recircumscribe tribes and genera within Cucurbitaceae and present a more natural classification for this family. Our new system comprises 95 genera in 15 tribes, five of them new: Actinostemmateae, Indofevilleeae, Thladiantheae, Momordiceae, and Siraitieae. Formal naming requires 44 new combinations and two new names in Cucurbitaceae. Keywords Cucurbitoideae; Fevilleoideae; nomenclature; nuclear ribosomal ITS; systematics; tribal classification Supplementary Material Figures S1–S5 are available in the free Electronic Supplement to the online version of this article (http://www.ingentaconnect.com/content/iapt/tax).
    [Show full text]
  • Rebecca Grumet Nurit Katzir Jordi Garcia-Mas Editors Genetics and Genomics of Cucurbitaceae Plant Genetics and Genomics: Crops and Models
    Plant Genetics and Genomics: Crops and Models 20 Rebecca Grumet Nurit Katzir Jordi Garcia-Mas Editors Genetics and Genomics of Cucurbitaceae Plant Genetics and Genomics: Crops and Models Volume 20 Series Editor Richard A. Jorgensen More information about this series at http://www.springer.com/series/7397 Rebecca Grumet • Nurit Katzir • Jordi Garcia-Mas Editors Genetics and Genomics of Cucurbitaceae Editors Rebecca Grumet Nurit Katzir Michigan State University Agricultural Research Organization East Lansing, Michigan Newe Ya’ar Research Center USA Ramat Yishay Israel Jordi Garcia-Mas Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Bellaterra, Barcelona Spain ISSN 2363-9601 ISSN 2363-961X (electronic) Plant Genetics and Genomics: Crops and Models ISBN 978-3-319-49330-5 ISBN 978-3-319-49332-9 (eBook) DOI 10.1007/978-3-319-49332-9 Library of Congress Control Number: 2017950169 © Springer International Publishing AG 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication.
    [Show full text]