Cucurbitaceae 2012 Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae October 15-18, 2012, Antalya-Turkey www.cucurbitaceae2012.org 1 2 EUROPEAN ASSOCIATION FOR RESEARCH ON PLANT BREEDING EUROPÄISCHE GESELLSCHAFT FÜR ZÜCHTUNGSFORSCHUNG EUCARPIA ASSOCIATION EUROPÉENNE POUR L’AMÉLIORATION DES PLANTES Cucurbitaceae 2012 Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae October 15-18th, 2012, Antalya - TURKEY Editors N. Sari I. Solmaz V. Aras Publisher: Çukurova University 3 Photograph on the front cover: N. Sari Organizing institution: Çukurova University, Faculty of Agriculture Department of Horticulture Adana, Turkey ISBN : 978-605-63297-0-8 Printed By : Bilkon Tourism & Travel Agency 4 President Prof. Dr. Nebahat Sari Çukurova University Honorary Presidents Prof. Dr. Kazım Abak Lefke European University Assoc. Prof. Dr. Masum Burak Director, Ministry of Food, Agriculture and Livestock Secretary Dr. İlknur Solmaz Çukurova University Scientific Committee Angela Davis Harris Moran Seed Company (USA) Maria J. Diez Niclos Universidad Politécnica de Valencia (Spain) Catherine Dogimont Institut National de la Recherche Agronomique (France) Hiroshi Ezura Tsukuba University (Japan) Xingfang Gu Chinese Academy of Agricultural Science (China) Jordi Garcia Mas Institut de Recerca i Tecnologia Agroalimentàries (Spain) Nurit Katzir Agricultural Research Organization (Israel) Elzbieta U. Kozik Research Institute of Vegetable Crops (Poland) Aleš Lebeda Palacky University (Czech Republic) Amnon Levi United States Dept. of Agriculture-Agricultural Research Service (USA) Harry Paris Agricultural Research Organization (Israel) Maria Belén Picó Universidad Politécnica de Valencia (Spain) Michel Pitrat Institut National de la Recherche Agronomique (France) Nebahat Sari Çukurova University (Turkey) Yaakov Tadmor Agricultural Research Organization (Israel) Todd Wehner North Carolina State University (USA) Yong Xu National Engineering Research Center for Vegetables (China) Halit Yetişir Erciyes University (Turkey) Xingping Zhang Syngenta (USA) 5 Organizing Committee Members Prof. Dr. Yeşim Yalçın Mendi Çukurova University Prof. Dr. H.Yıldız Daşgan Çukurova University Assoc. Prof. Dr. Yıldız Aka Kaçar Çukurova University Assist. Prof. Dr. Nezihe Köksal Çukurova University Dr. Abdullah Ünlü BATEM, Ministry of Food, Agriculture and Livestock Dr. Suat Yılmaz BATEM, Ministry of Food, Agriculture and Livestock Dr. Filiz Boyacı BATEM, Ministry of Food, Agriculture and Livestock Dr. Rana Kurum BATEM, Ministry of Food, Agriculture and Livestock Dr. Volkan Gözen BATEM, Ministry of Food, Agriculture and Livestock Dr. Asu Oğuz BATEM, Ministry of Food, Agriculture and Livestock MSc. Mine Ünlü BATEM, Ministry of Food, Agriculture and Livestock MSc. Yavuz Köksal BATEM, Ministry of Food, Agriculture and Livestock MSc. Veysel Aras ALATA, Ministry of Food, Agriculture and Livestock Res. Assist. Özhan Şimşek Çukurova University 6 PREFACE On behalf of the Organizing Committee of EUCARPIA Cucurbitaceae 2012, it gives me great pleasure to welcome you in Antalya, Turkey. The first EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae was organized by INRA, France 24 years ago. The following meetings were held in Poland (1992), Spain (1996), Isra- el (2000), Czech Republic (2004) and France (2008). It is a great honor and privilege for us to host the 10th EUCARPIA Cucurbitaceae Meeting in Antalya. Turkey is an important country in agricultural production and very rich in Cucurbit genetic resources. You will have the opportunity to see our genetic diver- sity in all Cucurbit species during our technical excursion. We expect more than 220 participants from 33 countries at the meeting in Rixos Downtown Hotel in Antalya. About 70% of the participants are from public research institutions and 30% are from private seed companies. We are pleased to welcome the international scientific community on cucurbit genetics, breeding and genomics. The proceeding contains 35 full text papers presented in oral sessions and 96 papers presented as posters at the Xth EUCARPIA Meeting on Genetics and Breed- ing of Cucurbitaceae. The authors should be thanked for their willingness to share their results. Our special thanks to scientific committee members for their contribution in evaluating oral and poster presentation and reviewing all the papers. We also thank the keynote speakers for their informative presentations. This meeting is vital for transferring results, stimulating new ideas for re- search topics, and in fostering co-operation possibilities. We would like to thank EUCARPIA, our public (Çukurova University, Ministry of Food, Agriculture and Livestock and Turkish Scientific and Technological Council) and private sponsors (Antalya Tarim, Manier Seed, Yuksel Seed, Syngenta, AG Seed, Fito Seed, Multi Seed, Nunhems and Rijk Zwaan) for accepting financial support of the meeting. We are grateful to the public institutions and private companies taking place in the technical trip. We also thank Bilkon Tourism and Travel Agency for their excellent service and organization. We will do our best to make Cucurbitaceae 2012 a most informative and enjoyable experience for all participants. We hope you enjoy this meeting and your stay in Antalya, Turkey. Antalya, October 2012 Prof. Dr. Nebahat SARI On behalf of the Organizing Committee 7 The organization of this meeting has been possible by the financial support of: Çukurova University EUCARPIA TÜBİTAK Batı Akdeniz Agricultural Research Institute Alata Horticultural Research Station Antalya Tarım Manier Seed Yüksel Seed Syngenta AG Seed Fito Seed Multi Seed Rijk Zwaan Nunhems 8 TABLE OF CONTENTS AUTHORS INDEX……………………………………………………………… 21 ORAL PRESENTATIONS……………………………………………………… 29 Session I: GENETIC RESOURCES AND BREEDING Domestication and diversification of melon………………………………......... 31 M. Pitrat Exploiting genetic diversity in Citrullus spp. to enhance watermelon cultivars……...……………………………….........……………………………... 41 A. Levi, J.A. Thies, W. P. Wechter, C. Kousik, K. Ling, H. Harrison, A. Simmons, U.K. Reddy, P. Nimmakayala, Z. Fei, S. Mitchell, Y. Xu, K. Tadmor and N. Katzir Association mapping of some selected watermelon traits using molecular markers…………………………………………………………………………... 49 O. Gulsen, M. Yagcioglu, H. Yetisir, I. Solmaz and N. Sari Early viability assessment in mutant populations of Cucurbita pepo ………... 50 N. Vicente-Dólera, V. Pinillos, M. Moya, F. Rubira, D. Martínez-Valdivieso, M. Del Río-Celestino, B. Román and P. Gómez Promotion of growth and health of Styrian oil pumpkins (Cucurbita pepo var. styriaca) by bacterial broad-spectrum antagonists……………………….. 56 E. Adam, M. Fürnkranz, H. Müller, M. Grube, H. Huss, J. Winkler and G. Berg Variation for bitterness and other fruit traits in bitter gourd (Momordica charantia L.) collections………………………………......................................... 66 N.P.S. Dhillon and S. Phethin Session II: GENETIC RESOURCES AND BREEDING Mode of inheritance of bitterness and spine colour in cucumber fruits (Cucumis sativus L.) ……………………………….............................................. 70 M. Pitchaimuthu, O.P. Dutta, K.R.M. Swamy and K. Souravi Development of fusarium wilt-resistant F1 hybrids of bottle gourd (Lagenaria siceraria Standl.) for watermelon rootstocks……………………… 74 Y.C. Huh, W.M. Lee, H.C. Ko, D.K. Park, K.S. Park, H.J. Lee, S.G. Lee, and K.D.Ko Undulate leaf blade margin of patty pan (Cucurbita pepo var. patisonina Greb.) new spontaneous mutation………………………………........................ 83 A. Korzeniewska and K. Niemirowicz-Szczytt 9 Stability of fruit yield in watermelon genotypes tested in multiple US environments……………………………….........……………………………….. 84 M. Dia, T.C. Wehner, R. Hassell, D.S. Price, G.E. Boyhan, S. Olson, S. King, A.R. Davis, G.E. Tolla, J. Bernier and B. Juarez Occurrence of androgynous inflorescence in Luffa cylindrica exhibiting dominant monogenic inheritance……………………………….......................... 89 S.P. Singh, A. Kumar and D.K. Dubey Determination and analysis of drought resistance index for watermelon during germination stage ……………………………….........…………………. 98 J Ma, X. Zhang, Y. Zhang, C. Li and Y. Mo Session III: GENOMICS AND FRUIT QUALITY Dynamic characteristics of enzymes and transcriptome related to sugar metabolism and accumulation in sweet and non-sweet watermelon fruits…... 103 Y. Xu, S. Guo, J. Liu, Z. Fei, H. He, H. Zhang, Y. Ren, H. Sun, G. Gong, Y. Zheng, M. Huang and S. Zhong eth3.5 and eth6.3 control climacteric fruit ripening in the near isogenic line SC3-5-1…………………………………………………………………………… 112 J. Vegas, P. Rios, W. Sanseverino, A.J. Monforte and J. Garcia-Mas Effect of carpel primordia-targeted inhibition of ethylene perception on sex expression, fruit set, and fruit ripening in melon (Cucumis melo L.) …….…...116 J.T. Switzenberg, L. Fernando, S.A. Hammar and R. Grumet Enzyme activity and Cucurbitacin content in relation to fusarium wilt resistance in melon…………………………………………………………….….126 E. Madadkhah and M. Lotfi Session IV: PEST AND DISEASE RESISTANCE Fusarium wilt of watermelon: A historical review…………………………….. 136 R.D. Martyn Resistance of melon to Cucumber Vein Yellowing Virus (CVYV) ……..……….157 M. Pitrat, C. Wipf-Scheibel, D. Besombes, C. Desbiez and H. Lecoq Genetic studies on resistance to downy mildew in cucumber…………….……165 E.U. Kozik, U. Klosińska, T.C. Wehner Application of a new approach for characterization and denomination of races of cucurbit powdery mildews–a case study of Czech pathogen populations…………………………………………………………………..……