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EKSTRAKSI DAN AMPLIFIKASI DNA Dryobalanops Oblongifolia Drey DAN Dryobalanops Lanceolata Burck

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EKSTRAKSI DAN AMPLIFIKASI DNA Dryobalanops Oblongifolia Drey DAN Dryobalanops Lanceolata Burck 1 EKSTRAKSI DAN AMPLIFIKASI DNA Dryobalanops oblongifolia Drey DAN Dryobalanops lanceolata Burck SKRIPSI TAMARA SYAFINA LUBIS 151201050 DEPARTEMEN BUDIDAYA HUTAN FAKULTAS KEHUTANAN UNIVERSITAS SUMATERA UTARA MEDAN 2019 Universitas Sumatera Utara 1 EKSTRAKSI DAN AMPLIFIKASI DNA Dryobalanops oblongifolia Drey DAN Dryobalanops lanceolata Burck SKRIPSI Oleh : TAMARA SYAFINA LUBIS 151201050 Skripsi sebagai salah satu syarat untuk memperoleh gelar sarjana di Fakultas Kehutanan Universitas Sumatera Utara DEPARTEMEN BUDIDAYA HUTAN FAKULTAS KEHUTANAN UNIVERSITAS SUMATERA UTARA MEDAN 2019 Universitas Sumatera Utara 1 Universitas Sumatera Utara 2 PERNYATAAN ORISINALITAS Saya yang bertanda tangan di bawah ini: Nama : Tamara Syafina Lubis NIM : 151201050 Judul Skripsi : Ekstraksi Dan Amplifikasi DNA Dryobalanops oblongifolia Drey Dan Dryobalanops lanceolata Burck Menyatakan bahwa skripsi ini adalah hasil karya sendiri. Pengutipan-pengutipan yang penulis lakukan pada bagian-bagian tertentu dari hasil karya orang lain dalam penulisan skripsi ini, telah penulis cantumkan sumbernya secara jelas sesuai dengan norma, kaidah, dan etika penulisan ilmiah. Medan, Agustus 2019 Tamara Syafina Lubis 151201050 ii Universitas Sumatera Utara 1 ABSTRACT TAMARA SYAFINA LUBIS: DNA Extraction and Amplification of Dryobalanops oblongifolia Drey and Dryobalanops lanceolata Burck. Supervised by ARIDA SUSILOWATI and HENDALASTUTI RACHMAT. Dryobalanops oblongifolia Drey and Dryobalanops lanceolata Burck are types of wood that have high economic value and many benefits. The resin produced by the Dryobalanops tree often called as borneol crystal. Genetic studies on Dryobalanops are important for managing species sustainably and balancing conservation and production aspects. Therefore, this research is important as basic knowledge to determine suitable areas that can strengthen species and be used for further studies related to population genetics, phylogeny studies, or biogeographic aspects.DNA extraction was carried out by applying the CTAB (Cetyl Trimethyl Ammonium Bromide) method with a few minor modifications at several stages. There are 3 regions tested to strengthen targeted DNA, namely trnL-trnF, rbcL, and matK. From the research, it was determined that matK with annealing temperature of 49oC and trnL-trnF with annealing temperature of 50oC are primers that can provide clear bands and good amplification. Keywords: Dryobalanops oblongifolia Drey and Dryobalanops lanceolata Burck, trnL-trnF, rbcL,matK,PCR iii Universitas Sumatera Utara 1 ABSTRAK TAMARA SYAFINA LUBIS: Ekstraksi dan Amplifikasi DNA Dryobalanops oblongifolia Drey dan Dryobalanops lanceolata Burck. Di bawah bimbingan ARIDA SUSILOWATI dan HENTI HENDALASTUTI RACHMAT. Dryobalanops oblongifolia Drey dan Dryobalanops lanceolata Burck merupakan jenis kayu yang bernilai ekonomi tinggi dan memiliki banyak manfaat. Resin yang dihasilkan oleh pohonDryobalanops sering disebut kristal borneol. Studi genetik pada pohon Dryobalanops penting untuk mengelola spesies secara berkelanjutan dan menyeimbangkan aspek konservasi dan produksinya. Oleh karena itu, penelitian ini penting sebagai pengetahuan dasar untuk menentukan wilayah yang cocok yang dapat memperkuat spesies dan digunakan untuk studi lanjutan terkait dengan genetika populasi, studi filogeni, atau aspek biogeografi. Ekstraksi DNA dilakukan dengan menerapkan metode CTAB (Cetyl Trimethyl Ammonium Bromide) dengan beberapa modifikasi kecil pada beberapa tahap. Ada 3 wilayah yang diuji untuk memperkuat DNA yang ditargetkan, yaitu trnL-trnF, rbcL, dan matK. Dari penelitian, ditentukan bahwamatK dengan suhu annealing 49oC dan trnL-trnF dengan suhu annealing 50oC adalah primer yang dapat memberikan pita yang jelas dan amplifikasi yang baik. Kata Kunci : Dryobalanops oblongifolia Drey dan Dryobalanops lanceolata Burck, trnL-trnF, rbcL, matK, PCR iv Universitas Sumatera Utara 1 RIWAYAT HIDUP Penulis lahir di Kota Kisaran pada tanggal 22 Januari 1998. Penulis merupakan anak ketiga dari tiga bersaudara oleh pasangan Bapak Ferry Anshari Lubis dan Ibu Suzanna Nasution. Penulis memulai pendidikan di SD Negeri 017106 Kisaran Pada Tahun 2003, pendidikan tingkat Sekolah Menengah Pertama diSMP Negeri 6 Kisaran pada tahun 2009. pendidikan tingkat Sekolah Menengah Atas di SMA Negeri 2 Kisaran pada tahun 2012. Pada tahun 2015, penulis lulus di Fakultas Kehutanan USU jalur Seleksi Nasional Masuk Perguruan Tinggi Negeri (SNMPTN). Penulis memilih minat Departemen Budidaya Hutan. Semasa kuliah penulis merupakan anggota Rain Forest di Fakultas Kehutanan, Universitas Sumatera Utara. Penulis telah mengikuti Praktik Pengenalan Ekosistem Hutan (P2EH) dikawasan hutan dengan tujuan khusus (KHDTK) Pondok Buluh pada tahun 2017. Pada tahun 2018 penulis juga telah menyelesaikan Praktik Kerja Lapangan (PKL) di Pusat Penelitian dan Pengembangan Hutan Bagian Kerjasama Komatsu-FORDA Bogor. Pada tahun 2018 penulis melaksanakan penelitian dengan judul Ekstraksi dan Amplifikasi DNA Dryobalanops oblongifolia Drey dan Dryobalanops lanceolata Burck. Di bawah bimbingan Ibu Dr. Arida Susilowati S.Hut., M.Si dan Ibu Henti Hendalastuti Rachmat S.Hut., M.Si.,P.hD v Universitas Sumatera Utara 1 KATA PENGANTAR Puji syukur penulis ucapkan kehadirat Allah SWT atas rahmat dan karunia-Nya penulis dapat menyelesaikan skripsi ini. Skripsi ini berjudul “Ekstraksi dan Amplifikasi DNA Dryobalanops oblongifolia Drey dan Dryobalanops lanceolata Burck”. Penulisan skripsi ini merupakan syarat dalam mencapai gelar sarjana di Fakultas Kehutanan, Universitas Sumatera Utara. Penulis banyak menerima bimbingan, motivasi, saran dan juga doa dari berbagai pihak selama penulisan skripsi ini. Teristimewa dari kedua orang tua yang sangat penulis sayangi yaitu Ayahanda Ferry Anshari Lubis dan Ibunda Suzanna Nasution juga buat kakak Hafriza Utari Lubis dan abang Sahrony Zulan Lubis keluarga penulis yang tidak pernah berhenti memberikan kasih sayang, doa, dukungan, juga nasihat yang tulus sampai sekarang ini. Pada kesempatan ini penulis mengucapkan terima kasih kepada : 1. Ibu Dr. Arida Susilowati, S.Hut., M.Si dan Ibu Henti Hendalastuti Rachmat, S.Hut., M.Si., Ph.D selaku komisi pembimbing yang telah membimbing dan mengarahkan penulis serta memberikan berbagai masukan berharga kepada penulis dalam penyelesaian skripsi ini. 2. Ibu Dr. Anita Zaitunah, S.Hut., M.Sc sebagai Dosen Penguji I, Ibu Evalina Herawati, S.Hut., M.Si sebagai Dosen Penguji II dan Ibu Ridahati Rambey, S.Hut,. M.Si, sebagai Dosen Penguji III. 3. Ibu Siti Latifah, S.Hut., M.Si.,Ph.D selaku Dekan Fakultas Kehutanan Universitas Sumatera Utara. 4. Bapak Mohammad Basyuni, S.Hut., M.Si., Ph.D sebagai Ketua Departemen Budidaya Hutan dan Ibu Dr. Deni Elfiati, SP., MP selaku Sekretaris Departemen Budidaya Hutan Fakultas Kehutanan Universitas Sumatera Utara. 5. Bapak Ahmad Baiquni Rangkuti, S.Hut., M.Si yang bersedia memberikan dukungan materi dan moral untuk pelaksanaan dan penyusun hasil penelitian ini. 6. Laboratorium Genetik dan Molekuler Kehutanan, Dapartemen Silvikultur, Fakultas Kehutanan, Institut Pertanian Bogor yang telah memberi ijin sebagai lokasi penelitian dan atas semua bantuan yang telah diberikan kepada peneliti. 7. Partner penulis yaitu Mahdil Khairi dan seluruh sahabat penulis Tim Penelitian, Gadis Sampul, Tim PKL Litbang Bogor, HUT B 2015, BDH 2015 dan seluruh teman teman dan adik-adik junior yang telah membantu dan memberi semangat dalam proses penelitian. Penelitian ini terlaksanakan atas bantuan hibah penelitian dosen dari dana hibah Pusat Penelitian dan Pengembangan Hutan Bagian Kerjasama Komatsu-FORDA Bogor penulis berharap semoga skripsi ini memberikan manfaat ke berbagai pihak. Akhir kata penulis mengucapkan terima kasih. Medan, Agustus 2019 Tamara Syafina Lubis Universitas Sumatera Utara 1 DAFTAR ISI Halaman LEMBAR PENGESAHAN ............................................................................. i PERNYATAAN ORISINALITAS .................................................................. ii ABSTRACT ....................................................................................................... iii ABSTRAK ....................................................................................................... iv RIWAYAT HIDUP .......................................................................................... v KATA PENGANTAR ..................................................................................... vi DAFTAR ISI .................................................................................................... vii DAFTAR TABEL ............................................................................................ ix DAFTAR GAMBAR ....................................................................................... x DAFTAR LAMPIRAN .................................................................................... xi PENDAHULUAN Latar Belakang................................................................. ................................ 1 Tujuan Penelitian....................................................... ...................................... 3 Kegunaan Penelitian......................................................................................... 3 TINJAUAN PUSTAKA Taksonomi Pohon Dryobalanops ..................................................................... 4 Manfaat Pohon Dryobalanops .......................................................................... 5 Permasalahan Pohon Dryobalanops ................................................................. 6 Studi Genetika .................................................................................................
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