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Universidad Católica De Santa María UNIVERSIDAD CATÓLICA DE SANTA MARÍA FACULTAD DE CIENCIAS FARMACÉUTICAS, BIOQUÍMICAS Y BIOTECNOLÓGICAS ESCUELA PROFESIONAL DE FARMACIA Y BIOQUÍMICA “Evaluación del efecto antipirético y antiinflamatorio del extracto de hojas de Kageneckia lanceolata (lloque) en animales de experimentación, Arequipa 2016” Tesis presentada por las bachilleres en Farmacia y Bioquímica: CCAHUANA CHURATA, DELIA LARICO SANCHO, IVET ROXANA Para obtener el Título Profesional de Químico- Farmacéutico Asesor: Q.F. Fernando Torres Vela AREQUIPA – PERÚ 2017 II DEDICATORIAS A Dios, por darme la vida, buena salud, la fuerza y el coraje para hacer este sueño realidad y por estar en cada momento de mi vida. A mis padres Santiago y Irma, por su amor, su ejemplo, consejos y valores, por motivarme a seguir adelante y por todo el esfuerzo y sacrificio que permitieron que hoy culmine una gran etapa. A mis hermanos Mirian, Alan, A Mis abuelos (QEPD), por Tatiana y Cosme, por darme la quererme y apoyarme siempre, fortaleza para culminar mis esto también se lo debo a ellos y estudios a todos mis amigas(o) que nos brindaron su apoyo durante todo este proceso, y dándonos sus consejos incondicionalmente. Delia III Dedico y agradezco esta tesis a Dios por darme fuerza, fe y haberme guiado a lo largo de este camino; dándome la A mi padre Aquiles Larico por fortaleza para afrontar los todo el esfuerzo y sacrificio por diversos retos que se me brindarme todo el amor, la presentaron. comprensión el apoyo incondicional y la confianza en cada momento de mi vida todo esto es para ti porque eres un ser maravilloso. A mis hermanos Vanesa y Yerson quienes con sus palabras de aliento y buenos deseos me brindan su apoyo incondicional y seguir adelante con mis Con cariño dedico a mis seres sueños. queridos, por motivarme y darme la mano, porque creyeron en mí, brindándome su apoyo, gracias a ustedes, hoy puedo ver alcanzada una de mis metas. Ivet Roxana III AGRADECIMIENTOS Brindarles nuestra mayor gratitud a todos nuestros docentes del Programa Profesional de Farmacia y Bioquímica de la Universidad Católica de Santa María, por el tiempo brindado y todas las enseñanzas y consejos aportados a nuestra formación profesional y personal durante toda nuestra carrera universitaria. Agradecer a nuestro asesor Dr. Fernando Torres por el gran apoyo que nos brindó y por todos sus consejos que nos sirvieron para realizar y culminar este trabajo. A nuestros dictaminadores quienes se merecen nuestro especial reconocimiento por el interés mostrado por nuestra investigación y las sugerencias recibidas a cada uno de ellos Dr. José Villanueva Salas, Dr. Gonzalo Dávila del Carpio y Dra. Mocita de la fuente Torres, por sus sabios consejos, su ayuda incondicional y experiencia nos dieron la clave para culminar esta meta de manera satisfactoria, gracias por su valiosa participación en nuestra formación profesional por su orientación, paciencia y la adquisición de conocimientos que nos ofrecieron a lo largo de nuestra tesis. Expresar también nuestro agradecimiento al personal de laboratorio por su apoyo y colaboración durante la realización de nuestro trabajo de investigación. IV ÍNDICE DEDICATORIAS ................................................................................................. II AGRADECIMIENTOS ........................................................................................ III RESUMEN ....................................................................................................... IX ABSTRACT ....................................................................................................... XI INTRODUCC IÓN ................................................................................................ XIII OBJETIVOS ....................................................................................................... 01 HIPÓTESIS ....................................................................................................... 02 CAPÍTULO I MARCO TEÓRICO ........................................................................................... 03 1.1. Kageneckia lanceolata (lloque) ................................................................ 03 1.1.1. Nombre científico ........................................................................... 03 1.1.2. Nombres comunes .......................................................................... 03 1.1.3. Descripción botánica ...................................................................... 03 1.1.4. Distribución .................................................................................... 04 1.1.5. Taxonomía ...................................................................................... 04 1.1.6. Composición química ..................................................................... 04 1.1.7. Usos medicinales ............................................................................ 04 1.1.8. Otros usos ....................................................................................... 05 1.2. FIEBRE ..................................................................................................... 06 1.2.1. Termorregulación ........................................................................... 06 1.2.2. Definición ....................................................................................... 07 1.2.3. Fisiopatología ................................................................................. 08 1.2.4. Diagnóstico diferencial................................................................... 12 V 1.2.5. Etiologías ........................................................................................ 13 1.2.6. Semiología ..................................................................................... 13 1.3. INFLAMACIÓN ....................................................................................... 15 1.3.1. Concepto ........................................................................................ 15 1.3.2. Causas de la inflamación ................................................................ 16 1.3.3. Componentes de la reacción Inflamatoria ...................................... 16 1.3.3.1. Cambios Hemodinámicos ................................................ 17 1.3.3.1.1. Exudación de plasma ......................................... 18 1.3.3.1.2. Alteraciones al flujo laminar ............................. 18 1.3.3.2. Alteraciones de la permeabilidad .................................... 19 1.3.3.3. Modificaciones leucocitarias ........................................... 19 1.3.3.3.1. Marginación y adherencia ................................. 20 1.3.3.3.2. Conglomeración ................................................ 21 1.3.3.3.3. Quimiotaxis ....................................................... 22 1.3.3.3.4. Fagocitosis ......................................................... 23 1.3.4. Características Macroscópicas de la Inflamación ........................ 24 1.4. ÁCIDO ACETILSALICÍLICO ................................................................. 25 1.4.1. Farmacocinética ............................................................................. 26 1.4.2. Farmacodinamia: .......................................................................... 27 1.4.2.1. Mecanismo de acción ....................................................... 27 1.4.2.2. Acciones farmacológicas .................................................. 27 1.4.3. Indicaciones ................................................................................... 28 1.4.4. Posología ....................................................................................... 29 1.4.5. RAM (Reacción Adversa al Medicamento) .................................. 29 1.4.6. Contraindicaciones ........................................................................ 30 VI 1.4.7. Interacciones ................................................................................. 30 1.4.8. Toxicidad ....................................................................................... 30 1.5. FUNDAMENTOS DE LOS MODELOS FARMACOLOGICOS DE EVALUACIÓN .................................................................................................... 31 1.5.1. Antipirético ...................................................................................... 31 1.5.2. Antiinflamatorio .............................................................................. 31 CAPÍTULO II MATERIALES Y MÉTODOS........................................................................... 33 2.1. TIPO DE ESTUDIO ...................................................................................... 33 2.2. LUGAR DE REALIZACIÓN ........................................................................ 33 2.3. MATERIAL BIOLOGICO ............................................................................ 33 2.3.1. Muestra vegetal ............................................................................... 33 2.3.2. Muestra animal ................................................................................ 34 2.4. MATERIALES DE LABORATORIO .......................................................... 34 2.4.1. Material de vidrio ......................................................................... 34 2.4.2. Equipos de laboratorio ................................................................... 34 2.4.3. Reactivos y otros insumos ............................................................. 35 2.4.4. Material farmacológico ................................................................. 36 2.4.5.
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