DOCSLIB.ORG

Mutagenic Characteristics and Contribution of Polycyclic Aromatic Hydrocarbons to Mutagenicity of Concentrates from Municipal River Water by Blue Chitin Column

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Mutagenic Characteristics and Contribution of Polycyclic Aromatic Hydrocarbons to Mutagenicity of Concentrates from Municipal River Water by Blue Chitin Column 232 Journal of Health Science, 48(3) 232–241 (2002) Mutagenic Characteristics and Contribution of Polycyclic Aromatic Hydrocarbons to Mutagenicity of Concentrates from Municipal River Water by Blue Chitin Column Akira Nagai,*, a Yoshinao Kano,a Ryozo Funasaka,a and Katsuhiko Nakamurob aGifu Research Center for Public Health, 4–6 Akebono-cho, Gifu-shi, Gifu 500–8148, Japan and bFaculty of Pharmaceutical Sciences, Setsunan University, 45–1 Nagaotoge-cho, Hirakata-shi, Osaka 573–0101, Japan (Received January 9, 2002; Accepted February 26, 2002) The mutagenic characteristics of concentrates from environmental water such as the effluent from various treatment plants and river waters using a modified Blue Chitin column method were determined by Ames assay with Salmonella typhimurium TA98, YG1021 and YG1024. This was done to estimate the quantitative contribution rate of mutagenicity estimated from analyzed polycyclic aromatic hydrocarbons (PAHs) content in the same concen- trates to the mutagenicity of environmental water. Moreover, PAHs content and the mutagenicity of the river sedi- ment-extract were measured to elucidate the fate of PAHs in the river. Many kinds of environmental waters and river sediment possessed principally indirect frame-shift type mutagenicity. Concentrates from many kinds of environ- mental waters possibly contained aminoarenes assayed with YG1021 and YG1024. In many kinds of environmental waters, the contribution rates of mutagenic magnitude estimated from seventeen analyzed PAH contents to the mutagenic magnitude of these water concentrates in TA98 with S9 mix were recognized to be from 0.10 to 1.15%. However, the contribution rate of mutagenic magnitude estimated from ten analyzed PAHs contents to the mu- tagenic magnitude of the Arata River sediment extract in TA98 with S9 mix was 64.2%. The high concentration of PAHs in the river sediment suggested that hydrophobic PAHs in the water might easily accumulate in the river sediment after adsorbing to the suspended solid. Key words —–— polycyclic aromatic hydrocarbons, mutagenic magnitude, contribution rate, environmental water, Blue Chitin INTRODUCTION cd)perylene as possible carcinogenic pollution indi- cators of drinking-water.15) The surface water of municipal river water has In mutagenicity investigations on environmen- served as a source of supply water. This river water tal water, water samples need to be concentrated for contains a large amount of trace toxic organic pol- Ames assay. One of the concentration methods is lutants, because it receives a large quantity of waste that using a solid support able to trap selectively water drained from the activities of human life, sew- molecular type mutagenic materials. XAD-2 resin age treatment plants, industrial waste plants, agri- traps a wide range of spectra of soluble organic sub- culture and road runoff. Many studies have been re- stances in water. Blue Cotton, Blue Rayon and Blue ported in which municipal river water shows mu- Chitin which have ligand linked copper phthalocya- tagenicity1–14) and its character is derived from nine trisulphonate are capable of adsorbing PAHs polycyclic aromatic hydrocarbons (PAHs) including with three or more fused rings. These have been used nitro- and amino-derivatives.1–6) Recently, WHO as a preconcentration method in many mutagenicity established the guidelines for fluoranthene, investigations.1–14,16–21) benzo(b)fluoranthene, benzo(k)fluoranthene, benzo Yamauchi et al.2) and Sasaki et al.13) used the (a)pyrene, benzo(ghi)peryrene and indeno(1,2,3- Blue Cotton hanging method for concentration of *To whom correspondence should be addressed: Gifu Research organic substances in river water, measured the mu- Center for Public Health, 4–6 Akebono-cho, Gifu-shi, Gifu 500– tagenicity of concentrate and analyzed 10 kinds of 8148, Japan. Tel.: +81-58-247-1300; Fax: +81-58-248-0229; E- PAHs, Trp-P-1 and Trp-P-2, respectively. Ohe and mail: [email protected] Nukaya reported the genotoxicity and the content No. 3 233 of 1-nitropyrene in concentrates from river water The column (14 mm i.d. × 60 mm) was packed using the XAD-2 resin column.17) Mouri et al. re- with 200 mg of Blue Chitin (Funakoshi, Ltd., Ja- ported the genotoxicity and the content of 6 kinds pan) which was in a powdered form and contained of heterocyclic amines in concentrates not only from 40 µmol of copper phthalocyanine trisulfonate- river water, but also from the effluent from various chitin. treatment plants using the Blue Rayon column Sample —–— The effluents from a night soil treat- method.19) ment plant, sewage treatment plant and septic tanks These reports described the contribution of PAHs combined with aerobic treatment were sampled in or heterocyclic amines to the mutagenicity or the Gifu city on September 1998. genotoxicity of river water, however, few target com- The surface waters of the Katsura River, the Uji pounds contributing to the mutagenicity or the River and the Kizu River were sampled on Septem- genotoxicity have been reported, and recovery of ber 1997. The surface waters of the Nagara River, such compounds by preconcentration method was the Sakai River, the Arata River and the Kuwabara not quantitative. River were sampled on September 1998. In the previous paper20) we reported a quantita- These water samples were filtered through a tive preconcentration method for 26 kinds of PAHs glass-fiber filter as quickly as possible after collec- in water, and suggested that a modified Blue Chitin tion. column method was useful for 14 kinds of PAHs with The Arata River sediment was sampled on May 4 and 5 rings. Moreover, we indicated the occurrence 1999. The sample was dried in a dark place at room of PAHs surveyed by GC-MS in the effluent from temperature, and was screened through a 2 mm mesh various treatment plants and river water. sieve. In this paper we measured 17 kinds of PAHs in- Preconcentration Method by Modified Blue cluding 6 kinds of PAHs established as WHO’s Chitin Column Method20) —–— Five liters of efflu- guideline in the environmental water and investi- ents from various treatment plants and river waters gated the mutagenic characteristics of concentrates was passed through a Blue Chitin column, which obtained from the effluent from various treatment was washed11) at a flow rate of 20 ml/min with a peri- plants and river water using this same method. We staltic pump, and then the column was washed with evaluated in detail quantitative contribution of mu- 20 ml of water. The organics adsorbed to Blue Chitin tagenic magnitude estimated from analyzed PAH were consecutively eluted with 100 ml of contents in the same concentrates to the mutagenic CH3OH • NH4OH (50 : 1) and 20 ml of CH2Cl2 at magnitude of river water concentrates as well as the a flow rate of 5 ml/min. Fractions of both river sediment. CH3OH • NH4OH (50 : 1) and CH2Cl2 were com- bined and evaporated to dryness with a rotary evapo- rator in a water bath at about 40°C under vacuum. MATERIALS AND METHODS The residue was dissolved in 2 ml of DMSO for Ames assay. The concentrates from upstream and Chemicals —–— downstream of the Nagara River were dissolved in Chemical sources were as follows: acenaphthylene 1 ml of DMSO, respectively. (Anl), acenaphthene (An), fluorene (Fl), phenan- Fifty grams of the river sediment was extracted threne (Phe), anthracene (Ant), fluoranthene (FA), with 200 ml of CH2Cl2 •C2H5OH (4 : 1) twice un- pyrene (PY), benz[a]anthracene (BaA), chrysene der ultrasonic vibration (28 Hz) for 15 min and then (Chr), benzo[a]pyrene (BaP) and dibenz[a,h] an- centrifuged at 3000 rpm for 10 min. The extract was thracene (diBahA) were from Wako Pure Chemical concentrated to about 100 ml with a rotary evapora- Industries, Ltd.; benzo[ghi]perylene (BghiP), 2- tor in a water bath at about 40°C under vacuum, aminoanthracene (2-AAnt), 3-aminofluoranthene (3- washed with 300 ml of 5% NaCl solution, and evapo- AFA) and 1-aminopyrene (1-APY) were from rated to dryness with a rotary evaporator in a water Aldrich Chemical Co. Inc.; benzo[b]fluoranthene bath at about 40°C under vacuum.21) The residue was (BbFA) and benzo[k]fluoranthene (BkFA) were from dissolved in 10 ml of CH3OH and then 90 ml of H2O RK Chemical Co.; methanol (CH3OH), ammonia added. This solution was passed through a Blue (NH4OH), dichloromethane (CH2Cl2) and dimethyl Chitin column and the same condition as for water sulfoxide (DMSO) were also from Wako Pure sample was followed. Half amount of concentrate Chemical Industries, Ltd., Japan. was dissolved in 2 ml of DMSO for Ames assay and 234 Vol. 48 (2002) Fig. 1. Mutagenicity of Concentrates from Effluent from Various Treatment Plant in TA98, YG1021 and YG1024 The numbers of spontaneous revertants per plate 25 ± 6.0 (TA98-S9), 33 ± 3.9 (TA98+S9), 20 ± 3.3 (YG1021-S9), 32 ± 5.7 (YG1021+S9), 23 ± 5.0 (YG1024-S9) and 60 ± 12 (YG1024+S9) were subtracted. the other amount was dissolved in 1 ml of CH2Cl2 for GC-MS analysis. GC-MS Analysis —–— Seventeenkinds of PAHs were analyzed by GC-MS using a Thermoquest GCQ. The conditions of GC-MS were described previously.20) Ames Salmonella/Microsome Assay22) —–— T h e bacterial Salmonella typhimurium strains used in the Ames Salmonella/microsome assay were TA98 and derivatives of TA98 capable of detecting mutagenic nitroarenes or aminoarenes: YG1021 and YG1024, respectively.23,24) YG1021 is a nitroreductase-over- producing strain and is highly sensitive to the mu- tagenic action of typical nitroarenes. YG1024 is an O-acetyltransferase-overproducing strain and is also Fig. 2. Mutagenic Magnitude of Concentrates from Effluent from Various Treatment Plants in TA98, YG1021 and extremely sensitive to the mutagenic action of YG1024 nitroarenes and aminoarenes. Ames assay was carried out by the liquid prein- cubation procedure.
Load more

Recommended publications
  • Muko City, Kyoto
    Muko city, Kyoto 1 Section 1 Nature and(Geographical Environment and Weather) 1. Geographical Environment Muko city is located at the southwest part of the Kyoto Basin. Traveling the Yodo River upward from the Osaka Bay through the narrow area between Mt. Tenno, the famous warfield of Battle of Yamazaki that determined the future of this country, and Mt. Otoko, the home of Iwashimizu Hachimangu Shrine, one of the three major hachimangu shrines in Japan, the city sits where three rivers of the Katsura, the Uji and the Kizu merge and form the Yodo River. On west, Kyoto Nishiyama Mountain Range including Mt. Oshio lays and the Katsura River runs on our east. We share three boundaries with Kyoto city - the northern and western boundaries with Nishikyo-ku, and the eastern boundary with Minami-ku and Fushimi-ku. Across the southern boundary is Nagaokakyo city abutting Oyamazaki-cho which is the neighbor of Osaka Prefecture. The city is approximiately 2km from east to west and approximiately 4km from south to north covering the 7.72km2 area. This makes us the third smallest city in Japan after Warabi city and Komae city. Figure 1-1-1 Location of Muko city （Right figure （Kyoto map） ： The place of red is Muko city） （Lower figure （Japan map） ： The place of red is Kyoto） N W E S 1 Geographically, it is a flatland with the northwestern part higher and the southwestern part lower. This divides the city coverage into three distinctive parts of the hilly area in the west formed by the Osaka Geo Group which is believed to be cumulated several tens of thousands to several million years ago, the terrace in the center, and the alluvial plain in the east formed by the Katsura River and the Obata River.
    [Show full text]
  • Geomorphological Evolution and Sediment Budget Analysis with the Uji River, Kyoto
    D-7 Fourth International Conference on Scour and Erosion 2008 GEOMORPHOLOGICAL EVOLUTION AND SEDIMENT BUDGET ANALYSIS WITH THE UJI RIVER, KYOTO Ryoukei AZUMA1 and Hideo SEKIGUCHI2 1Member of ISSMGE, Assistant Professor, Disaster Prevention Research Institute, Kyoto University (Shimomisu, Yoko-oji, Fushimi-ku, Kyoto 612-8235, Japan) E-mail:[email protected] 2 Member of ISSMGE, Professor, Disaster Prevention Research Institute, Kyoto University (Shimomisu, Yoko-oji, Fushimi-ku, Kyoto 612-8235, Japan) E-mail:[email protected] This paper starts with presenting field evidences that illustrate the occurrence of significant bank erosion in a reach of the Uji River. It then demonstrates that the resulting escarpment was not a mere local adjustment, but is an indicator as to how extensively the Uji River has undergone appreciable amounts of channel erosion over the entire 15.2km-long section. The datasets on which the discussion is based include: the cross-channel topographical data (200m intervals along the river course) in 1967, 1979 and 2006 made available from the Yodogawa Office, MLIT. A careful geomorphological analysis of the datasets in terms of GIS, permitted the determination of tempo-spatial changes of areal sediment storage in the Uji River. When integrated over the entire river course, the overall volume of sediment loss due to erosion proved to amount to 3.1 ×106 m3 in the period from 1967 up to 2006. A discussion is made to identifying its practical implications for future riverine management. Furthermore, a discussion is made of the linkage between the extent of the channel erosion and the depositional environments of the sediment that constitutes the boundary of the river channel.
    [Show full text]
  • Keys to the Flesh Flies of Japan, with the Description of a New Genus And
    〔Med. Entomol. Zool. Vol. 66 No. 4 p. 167‒200 2015〕 167 reference DOI: 10.7601/mez.66.167 Keys to the esh ies of Japan, with the description of a new genus and species from Honshu (Diptera: Sarcophagidae) Hiromu Kurahashi*, 1) and Susumu Kakinuma2) * Corresponding author: [email protected] 1) Department of Medical Entomology, National Institute of Infectious Diseases, Toyama 1‒23‒1, Shinjuku-ku, Tokyo 162‒8640 Japan 2) IDD Yamaguchi Lab., Aobadai 11‒22, Yamaguchi-shi, Yamaguchi 753‒0012 Japan (Received: 9 June 2015; Accepted: 2 October 2015) Abstract: A new genus and species of the Japanese Sarcophagidae, Papesarcophaga kisarazuensis gen. & sp. nov. is described and illustrated from Honshu, Japan. Practical keys to the Japanese 43 genera and 122 species are provided including this new species. A check list and data of specimens examined are also provided. Key words: Diptera, flesh flies, new species, new genus, Sarcophagidae, Japan INTRODUCTION The collection of Sarcophagidae made by the first author was studied during the course of the taxonomical studies on the calypterate muscoid flies from Japan since 1970 (Kurahashi, 1970). This was a revision of the subfamily Miltogramatinae dealing with seven genera and 14 species. Before this, Takano (1950) recorded seven genera and nine species of Japanese Sarcophagidae. Many investigation on the Japanese flesh flies made by Drs. K. Hori, R. Kano and S. Shinonaga beside the present authors. The results of these authors were published in the part of Sacophagidae, Fauna Japanica (Insecta: Diptera) and treated 23 genera and 65 species of the subfamilies of Sarcophaginae and Agriinae (=Paramacronychiinae), but the subfamily Miltogrammatinae was not included (Kano et al., 1967).
    [Show full text]
  • Mie Aichi Shizuoka Nara Fukui Kyoto Hyogo Wakayama Osaka Shiga
    SHIZUOKA AICHI MIE <G7 Ise-Shima Summit> Oigawa Railway Steam Locomotives 1 Toyohashi Park 5 The Museum Meiji-mura 9 Toyota Commemorative Museum of 13 Ise Grand Shrine 17 Toba 20 Shima (Kashikojima Island) 23 These steam locomotives, which ran in the This public park houses the remains of An outdoor museum which enables visitors to 1920s and 1930s, are still in fully working Yoshida Castle, which was built in the 16th experience old buildings and modes of Industry and Technology order. These stations which evoke the spirit century, other cultural institutions such as transport, mainly from the Meiji Period The Toyota Group has preserved the site of the of the period, the rivers and tea plantations the Toyohashi City Museum of Art and (1868–1912), as well as beef hot-pot and other former main plant of Toyoda Automatic Loom the trains roll past, and the dramatic History, and sports facilities. The tramway, aspects of the culinary culture of the times. The Works as part of its industrial heritage, and has mountain scenery have appeared in many which runs through the environs of the park museum grounds, one of the largest in Japan, reopened it as a commemorative museum. The TV dramas and movies. is a symbol of Toyohashi. houses more than sixty buildings from around museum, which features textile machinery and ACCESS A 5-minute walk from Toyohashikoen-mae Station on the Toyohashi Railway tramline Japan and beyond, 12 of which are designated automobiles developed by the Toyota Group, ACCESS Runs from Shin-Kanaya Station to Senzu on the Oigawa Railway ACCESS A 20-minute bus journey from as Important Cultural Properties of Japan, presents the history of industry and technology http://www.oigawa-railway.co.jp/pdf/oigawa_rail_eng.pdf Inuyama Station on the Nagoya Railroad which were dismantled and moved here.
    [Show full text]
  • FY2017 Results of the Radioactive Material Monitoring in the Water Environment
    FY2017 Results of the Radioactive Material Monitoring in the Water Environment March 2019 Ministry of the Environment Contents Outline .......................................................................................................................................................... 5 1) Radioactive cesium ................................................................................................................... 6 (2) Radionuclides other than radioactive cesium .......................................................................... 6 Part 1: National Radioactive Material Monitoring Water Environments throughout Japan (FY2017) ....... 10 1 Objective and Details ........................................................................................................................... 10 1.1 Objective .................................................................................................................................. 10 1.2 Details ...................................................................................................................................... 10 (1) Monitoring locations ............................................................................................................... 10 1) Public water areas ................................................................................................................ 10 2) Groundwater ......................................................................................................................... 10 (2) Targets ....................................................................................................................................
    [Show full text]
  • 資料29－4 Ihpトレーニングコース2014概要,2013
    International Hydrological Programme International Hydrological Programme Forest Hydrology Conservation of Forest, Soil, and Water Resource The Twenty-fourth IHP Training Course 24 November - 7 December, 2014 Nagoya, Japan Hydrospheric Atmospheric Research Center, Nagoya University Supported by Water Resources Research Center, Disaster Prevention Research Institute, Kyoto University Outline A short training course “Forest Hydrology Conservation of Forest, Soil, and Water Resource” will be programmed for participants from Asia-Pacific regions as a part of Japanese contribution to the International Hydrological Program (IHP). The course is composed of a series of lectures and practice sessions. Objectives Incident rainwater is firstly intercepted by foliage and branches and evaporates from their surface to the atmosphere. From the surface full of rainwater, the rainwater conducts to soils along the plant body, and also, some of incident rainwater directly reaches the soils without touching foliage and branches. These rainwaters coming to the soils infiltrate into the soils with being influenced by the soil pore structure, and reaching to groundwater table. During the slow soil water movement, some of the soil water is absorbed by plant roots. It conducts through stem conduits, reaches leaves, and evaporates to the atmosphere through stomata. The ground water flows to a river. This is an outline of water cycling in the forest ecosystems, and science on this water cycling is “Forest Hydrology”. As one can see above, Forest Hydrology has two major scientific aspects, i.e., discharge from forested watershed and water use by trees (evaporation from the forest canopy). Soils in the forested watershed have a high hydraulic conductivity at the ground surface, which prevents from soil erosion, and functions to make soil water flow slow, resulting in a behavior like a dam.
    [Show full text]
  • IWRM Guidelines at River Basin Level, Part
    )3". 0HOTOCREDITS #OVERÚ!NDRÖS3ZLLSI .AGYÚ)&!$(ORST7AGNERÚ#")+9IN,UN Ú5.%3#/Ú#OUTERON 6AL£RIEÚ*APAN7ATER!GENCYÚ"'AJDA IWRM Guidelines at River Basin Level PART 2-1 The Guidelines for IWRM Coordination GUIDELINES vol. 2-1.indd 1 24/04/09 18:58:35 TABLE OF CONTENTS 1 FEATURES AND STRUCTURE OF 4 IWRM PROCESS ............................................................... 51 ‘THE GUIDELINES FOR IWRM COORDINATION’ .....3 4.1 The ‘IWRM spiral’ conceptual model ...........................51 1.1 Features .................................................................................3 4.1.1 The IWRM spiral .............................................................. 51 1.2 Structure ...............................................................................3 4.1.2 Stages of the IWRM spiral ............................................ 52 4.1.3 Recognizing the stage shift ............................................ 52 2 SECTORAL PERSPECTIVES IN IWRM ........................5 4.1.4 Where do you stand in the spiral? ............................... 52 2.1 Good-understanding of sectoral perspectives 4.2 Phases, steps and keys for success and their relationships is key for IWRM ...............................5 in the IWRM process .............................................................53 2.2 Water users’ perspectives .................................................5 4.2.1 Phase 1: Recognizing and identifying .......................... 54 2.2.1 Agricultural sector .................................................................5 4.2.2
    [Show full text]
  • NIAES Annual Report 2012
    2012 Annual Report 2012 (April 2011 ‒ March 2012) National Institute for Agro-Environmental S ciences About the symbol..... The symbol’s colors represent the research domains of NIAES: the sky is light blue, clouds and water are white, biota are green, and soils are brown. Editorial Board Chairman: Kiyotaka Miyashita President Vice Chairman: Mitsunori Oka Vice President Editorial Committee: Akira Hasebe Principal Research Director Yasuhiro Yogo Principal Research Coordinator Toshihiro Kadosawa Head, Public Relations and Information Office Katsutoshi Yamamoto Head, Research Planning Office Sakuji Kawamura Head, Accounting Office Hitoshi Toritani Director, Ecosystem Informatics Division Takashi Otani Director, Organochemicals Division Takeshi Fujii Director, Environmental Biofunction Division Contents Message from the President ………………………………………………………… 2 Basic Philosophy, Code of Conduct, and Environmental Charter … 3 History of NIAES ………………………………………………………………………… 4 Research Overview in FY2011 ……………………………………………………… 5 Research Organization ……………………………………………………………………… 6 Summary of NIAES Research Projects …………………………………………………… 7 Plans and Results of Research Projects with External Competitive Funding …………… 26 Major Symposia and Seminars……………………………………………………………… 29 Research Collaborations …………………………………………………………………… 37 Evaluation Committee FY2011 …………………………………………………………… 38 Academic Prizes and Awards ……………………………………………………………… 39 Career Building and Outreach Programs…………………………………………………… 41 International Activities Foreign Visitors ……………………………………………………………………………
    [Show full text]
  • NAGOYA UNIVERSITY GRADUATE SCHOOL of LAW Doctoral
    NAGOYA UNIVERSITY GRADUATE SCHOOL OF LAW Doctoral Dissertation ADMINISTRATIVE RESPONSIBILITY FOR WATER POLLUTION PREVENTION IN LAOS: LESSONS LEARNED FROM JAPAN A Dissertation Submitted in Fulfillment of the Requirements for the Degree of Doctor of Laws Course: LL.D. (Comparative Law) Program in Law and Political Science 2016-2019 Name of the Student: DONKEODAVONG Latdavanh Student ID Number: 431604065 Main Academic Supervisor: Professor INABA Kazumasa Sub-Academic Supervisor: Professor ICHIHASHI Katsuya Sub-Academic Supervisor: Professor FUKASAWA Ryuichiro Date of Submission: July 26, 2019 Abstract This research project investigates the administrative responsibility for water pollution prevention in Laos by referring to Japan. The main aim of this study is to answer three main research questions. First, how does the legal system in Laos fail to prevent water pollution? Second, what are aspects of other countries’ legal systems worthy of reference for Laos? Lastly, how is administrative responsibility in Japan worthy of reference in order to find legal issues for Laos? In order to answer these research questions, this paper consists of four chapters, an introduction, and conclusion. Chapter I attempts the reason for paying attention to the concept of responsibility, which is a “leading thread” of this study. This chapter compares the general differences between the Japanese and Laotian legal systems. Chapter II describes the history and characteristics of the water pollution prevention law in Laos. Chapter III analyzes the development of the water pollution prevention law in Japan in order to discover the problems of the Laotian legal system, as described in Chapter II. Based on this analysis, Chapter IV presents the problems of the water pollution prevention law in Laos.
    [Show full text]
  • Tourist Guidebook (Pdf)
    Tourist Guidebook UNESCO World Cuitural Heritage Site All information contained in this book is based on data as of Feb. 1, 2005 and is subject to change without notice. Kyoto Convention Bureau Kibune Shrine A brief over view Kurama Kibuneguchi of the city Ninose Sanzen-inTemple Ichihara Nikenjaya Kyoto Seika University Kino Iwakura Rakuhoku Hachiman-mae Yase-yuen Sta. Kokusaikaikan Kamigamo Miyake Hachiman Shrine Takaragaike Kozanji Temple Kitayama-dori Shugakuin Imperial Villa Kitayama Matsugasaki Syugakuin Kitaoji Kyoto Imperial Palace Ichijoji Kinkakuji karasuma-dori Temple Kitaoji-dori Rakusai Shimogamo Chayama Shrine Shirakawa-dori Ryoanji Temple Kuramaguchi Mototanaka Ninnaji Temple Saga-Arashiyama Rakuchu Kawaramachi-dori Kawabata-dori Demachiyanagi Imadegawa Higashioji-dori ilwa Line a y o Imadegawa-dori Horikawa-dori K Senbon-dori n Toji-in i Kitanohakubaicho R t a Ryoanji-michi Takaoguchi Omuro u Myoshinji k Subway Karasuma Line Subway u Narutaki f i Ginkakuji Uzumasa Ke Temple Tokiwa JR c h Sanin a Marutamachi-dori Sanjo Keihan Higashiyama i i Main Line c Arashiyama Hanazono Enmachi Marutamachi Marutamachi Sanjo-guchi Rokuoin Kurumazaki Sagaeki-mae Nijo Ke Arisugawa Katabiranotsuji ifuku Castle Kyoto Tenryuji Ra Nijo i ne Uzumasa lw Heian Jingu Rakuto Temple a Oike-dori Shiyakushomae y Nijojomae Oike Karasuma Kaikonoyashiro A Shrine mae rashiyama Line Nijo Yamanouchi Sanjo- Keage Arashiyama Sanjo Subway Tozai Line Tenjingawa-dori dori Shijo-dori YYasakaasaka JJinjainja Karasuma Kawaramachi Omiya Shijo Saiin Saiin Shijo-omiya
    [Show full text]
  • Program Book
    DVORRUJMDSDQ Association for the Sciences of Limnology and Oceanography Meeting Program ASLO Contents Welcome! ..........................................................................................2 Conference Events ........................................................................12 Meeting Sponsors ...........................................................................2 Public Symposium on Global Warming...........................................12 Meeting Supporters .......................................................................2 Opening Welcome Reception.............................................................12 Organizing Committee .................................................................2 ASLO Membership Business Meeting .............................................12 Poster Sessions and Receptions .........................................................12 Co-Chairs ..................................................................................................2 Scientifi c Committee ..............................................................................2 Workshops and Town Hall Meetings ......................................12 Local Organizing Committee ...............................................................2 L&O e-Lectures Town Hall Meeting ................................................12 Advisory Committee ..............................................................................2 Workshop: Th e Future of Ecosystems Science ...............................12 ASLO Student
    [Show full text]
  • A Revised and Updated Checklist of the Parasites of Eels (Anguilla Spp.) (Anguilliformes: Anguillidae) in Japan (1915-2017)
    33 69 生物圏科学 Biosphere Sci. 56：33－69 (2017) A revised and updated checklist of the parasites of eels (Anguilla spp.) (Anguilliformes: Anguillidae) in Japan (1915-2017) 1) 2) Kazuya NAGASAWA and Hirotaka KATAHIRA 1) Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan 2) Faculty of Bioresources, Mie University, 1577 Kurima machiya-cho, Tsu, Mie 514-8507, Japan Published by The Graduate School of Biosphere Science Hiroshima University Higashi-Hiroshima 739-8528, Japan November 2017 生物圏科学 Biosphere Sci. 56：33－69 (2017) REVIEW A revised and updated checklist of the parasites of eels (Anguilla spp.) (Anguilliformes: Anguillidae) in Japan (1915-2017) 1) 2) Kazuya NAGASAWA * and Hirotaka KATAHIRA 1) Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan 2) Faculty of Bioresources, Mie University, 1577 Kurima machiya-cho, Tsu, Mie 514-8507, Japan Abstract Information on the protistan and metazoan parasites of four species of eels (the Japanese eel Anguilla japonica, the giant mottled eel Anguilla marmorata, the European eel Anguilla anguilla, and the short-finned eel Anguilla australis) in Japan is summarized in the Parasite-Host and Host- Parasite lists, based on the literature published for 103 years between 1915 and 2017. This is a revised and updated version of the checklist published in 2007. Anguilla japonica and A. marmorata are native to Japan, whereas A. anguilla and A. australis are introduced species from Europe and Australia, respectively. The parasites, including 54 nominal species and those not identified to species level, are listed by higher taxa as follows: Sarcomastigophora (no.
    [Show full text]