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WO 2018/005411 Al 04 January 2018 (04.01.2018) W!P O PCT

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(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2018/005411 Al 04 January 2018 (04.01.2018) W!P O PCT (51) International Patent Classification: (72) Inventors: BARRY, Jennifer; 7250 NW 62ND Av AOlH l/00 (2006.01) C12N 15/09 (2006.01) enue, PO BOX 552, Johnston, Iowa 5013 1-0552 (US). AOlH S/00 (2006.01) C12N 15/31 (2006.01) FINKE, Catherine; 7250 NW 62ND Avenue, PO BOX A01N 63/00 (2006.01) 552, Johnston, Iowa 5013 1-0552 (US). GERBER, Ryan; 2824 Brighton Bluff Drive, Apex, North Carolina 27539 (21) International Application Number: (US). LUM, Amy; 7250 NW 62ND Avenue, PO BOX PCT/US2017/039376 552, Johnston, Iowa 50131-0552 (US). MATHIS, John; (22) International Filing Date: 7250 NW 62ND Avenue, PO BOX 552, Johnston, Iowa 27 June 2017 (27.06.2017) 5013 1-0552 (US). ONG, Azalea; 7250 NW 62ND Av enue, PO BOX 552, Johnston, Iowa 5013 1-0552 (US). PE- (25) Filing Language: English TERSON-BURCH, Brooke; 7250 NW 62ND Avenue, (26) Publication Language: English PO BOX 552, Johnston, Iowa 5013 1-0552 (US). WOLFE, Thomas C ; 7250 NW 62ND AVE, PO BOX 552, John (30) Priority Data: ston, Iowa 5013 1-0552 (US). XIE, Weiping; 7250 NW 62/357,501 0 1 July 2016 (01 .07.2016) US 62ND Avenue, PO BOX 552, Johnston, Iowa 5013 1-0552 (71) Applicant: PIONEER HI-BRED INTERNATIONAL, (US). YALPANI, Nasser; 7250 NW 62ND Avenue, PO INC. [US/US]; 7100 N.W. 62nd Avenue, P.O. Box 1014, BOX 552, Johnston, Iowa 50131-0552 (US). ZHONG, Xi- Johnston, Iowa 5013 1-1014 (US). aohong; 7250 NW 62ND Avenue, PO BOX 552, Johnston, Iowa 5013 1-0552 (US). (54) Title: INSECTICIDAL PROTEINS FROM PLANTS AND METHODS FOR THEIR USE Fig. 2 90 o 80 E 60 u + 50 40 o 30 20 10 ECB CEW FA ECB CEW FAW EC CEW FAW ECB CEW FAW ECB CEW FAW PHP79658 PHP79659 PHP7 6S P s Control eg Control (57) Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression © vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or © partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, 00 Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity. o o [Continued on nextpage] WO 2018/005411 Al llll II II 11III II I II III I III II il II I II (74) Agent: BAUER, S. Christopher; PIONEER HI-BRED IN¬ TERNATIONAL, INC., 7100 N.W. 62nd Avenue, P.O. Box 1014, Johnston, Iowa 5013 1-1014 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) INSECTICIDAL PROTEINS FROM PLANTS AND METHODS FOR THEIR USE CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to U.S. provisional patent application Serial Number 62/357,501 filed July 0 1 , 2016, herein incorporated by reference in its entirety. REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named "6762WOPCT_Sequencel_isting" created on June 1, 2017, and having a size of 546 kilobytes and is filed concurrently with the specification. The sequence listing contained in this ASCII formatted document is part of the specification and is herein incorporated by reference in its entirety. FIELD This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. BACKGROUND Biological control of insect pests of agricultural significance using a microbial agent, such as fungi, bacteria or another species of insect affords an environmentally friendly and commercially attractive alternative to synthetic chemical pesticides. Generally speaking, the use of biopesticides presents a lower risk of pollution and environmental hazards and biopesticides provide greater target specificity than is characteristic of traditional broad- spectrum chemical insecticides. In addition, biopesticides often cost less to produce and thus improve economic yield for a wide variety of crops. Certain species of microorganisms of the genus Bacillus are known to possess pesticidal activity against a range of insect pests including Lepidoptera, Diptera, Coleoptera, Hemiptera and others. Bacillus thuringiensis {Bt) and Bacillus popilliae are among the most successful biocontrol agents discovered to date. Insect pathogenicity has also been attributed to strains of B. larvae, B. lentimorbus, B. sphaericus and B. cereus. Microbial insecticides, particularly those obtained from Bacillus strains, have played an important role in agriculture as alternatives to chemical pest control. Crop plants have been developed with enhanced insect resistance by genetically engineering crop plants to produce pesticidal proteins from Bacillus. For example, corn and cotton plants have been genetically engineered to produce pesticidal proteins isolated from strains of Bacillus thuringiensis. These genetically engineered crops are now widely used in agriculture and have provided the farmer with an environmentally friendly alternative to traditional insect-control methods. While they have proven to be very successful commercially, these genetically engineered, insect-resistant crop plants provide resistance to only a narrow range of the economically important insect pests. In some cases, insects can develop resistance to different insecticidal compounds, which raises the need to identify alternative biological control agents for pest control. Accordingly, there remains a need for new pesticidal proteins with different ranges of insecticidal activity against insect pests, e.g., insecticidal proteins which are active against a variety of insects in the order Lepidoptera and the order Coleoptera including but not limited to insect pests that have developed resistance to existing insecticides. SUMMARY In one aspect compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding sequences for pesticidal and insecticidal polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. Compositions also include the pesticidal polypeptide sequences and antibodies to those polypeptides. Compositions also comprise transformed bacteria, plants, plant cells, tissues and seeds. In another aspect isolated or recombinant nucleic acid molecules are provided encoding IPD1 03 polypeptides including amino acid substitutions, deletions, insertions, and fragments thereof. Provided are isolated or recombinant nucleic acid molecules capable of encoding IPD1 03 polypeptides of SEQ ID NO: 2, SEQ ID NO: 4 , SEQ ID NO: 6 , SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36 or SEQ ID NO: 38, as well as amino acid substitutions, deletions, insertions, fragments thereof, and combinations thereof. Nucleic acid sequences that are complementary to a nucleic acid sequence of the embodiments or that hybridize to a sequence of the embodiments are also encompassed. The nucleic acid sequences can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. The nucleotide or amino acid sequences may be synthetic sequences that have been designed for expression in an organism including, but not limited to, a microorganism or a plant. In another aspect IPD103 polypeptides are encompassed. Also provided are isolated or recombinant IPD103 polypeptides of SEQ ID NO: 2, SEQ ID NO: 4 , SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36, and SEQ ID NO: 38, as well as amino acid substitutions, deletions, insertions, fragments thereof and combinations thereof.
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  • Boigu Islands, Form the Northern Island Group of Torres Strait, Located Approximately 150 Km North of Thursday Island (See Figure 1)

    Boigu Islands, Form the Northern Island Group of Torres Strait, Located Approximately 150 Km North of Thursday Island (See Figure 1)

    PROFILE FOR MANAGEMENT OF THE HABITATS AND RELATED ECOLOGICAL AND CULTURAL RESOURCE VALUES OF DAUAN ISLAND January 2013 Prepared by 3D Environmental for Torres Strait Regional Authority Land & Sea Management Unit Cover image: 3D Environmental (2013) EXECUTIVE SUMMARY The granite rock pile that forms Dauan, along with nearby Saibai and Boigu Islands, form the Northern Island Group of Torres Strait, located approximately 150 km north of Thursday Island (see Figure 1). Whilst Saibai and Boigu Island are extensions of the alluvial Fly Platform, geologically part of the Papua New Guinea mainland, Dauan is formed on continental basement rock which extends northward from Cape York Peninsula to Mabadauan Hill on the south-west coast of Papua New Guinea. A total of 14 vegetation communities, within ten broad vegetation groups and 14 regional ecosystems are recognised on the island. The total known flora of comprises 402 species (14 ferns, 388 angiosperms), with 317 native and 85 naturalised species. Nine plant species are considered threatened at the commonwealth and state levels and a further 25 species considered to have significance at a regional level. As for the majority of Torres Strait Islands there is a lack of systematic survey of fauna habitats on the island. A desktop review identified 135 fauna species that are reported to occur on Dauan. This can be compared with the 384 terrestrial fauna species that have been reported for the broader Torres Strait Island group. The Dauan fauna comprises 20 reptiles, 100 birds, 3 frogs and 12 mammals. Of these, one reptile, one bird and four mammal species are introduced.
  • Running Title: Bacterial Community Profiling Highlights Complex Diversity and Novel Organisms in Wildlife Ticks. Authors: Siobho

    Running Title: Bacterial Community Profiling Highlights Complex Diversity and Novel Organisms in Wildlife Ticks. Authors: Siobho

    bioRxiv preprint doi: https://doi.org/10.1101/807131; this version posted October 17, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Running title: Bacterial community profiling highlights complex diversity and novel 2 organisms in wildlife ticks. 3 4 Authors: Siobhon L. Egan1, Siew-May Loh1, Peter B. Banks2, Amber Gillett3, Liisa A. 5 Ahlstrom4, Una M. Ryan1, Peter J. Irwin1 and Charlotte L. Oskam1,* 6 7 1 Vector and Waterborne Pathogens Research Group, College of Science, Health, 8 Engineering and Education, Murdoch University, Perth, Western Australia, Australia 9 2 School of Life and Environmental Sciences, The University of Sydney, Sydney, New South 10 Wales, Australia 11 3 Australia Zoo Wildlife Hospital, Beerwah, Queensland, Australia 12 4 Bayer Australia Ltd, Animal Health, Pymble, New South Wales, Australia 13 * Corresponding author 14 15 S.L.E [email protected]; https://orcid.org/0000-0003-4395-4069 16 S-M.L. [email protected] 17 P.B.B. [email protected]; https://orcid.org/0000-0002-4340-6495 18 A.G. [email protected] 19 L.A.A [email protected] 20 U.M.R. [email protected]; https://orcid.org/0000-0003-2710-9324 21 P.J.I. [email protected]; https://orcid.org/0000-0002-0006-8262 22 C.L.O. c.o [email protected] ; https://orcid.org/0000-0001-8886-2120 23 24 Abstract 25 Ticks (Acari: Ixodida) transmit a greater variety of pathogens than any other blood-feeding 1 bioRxiv preprint doi: https://doi.org/10.1101/807131; this version posted October 17, 2019.