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Volume 23, Number 2, December 2017 The International Society for Southeast Asian Agricultural Sciences J. ISSAAS Vol. 23, No. 2 (2017) CONTENTS Contributed Papers Page Effect of trans-2-hexenal vapor pretreatment on alleviation of heat shock in tomato seedlings (Micro Tom) Naoki Terada, Atsushi Sanada, Hiroshi Gemma and Kaihei Koshio.................................... 1 Isolation, characterization and rapid screening of copper-tolerant cyanobacteria consortia from mining sites and strawberry farms in Benguet Province, Philippines Libertine Rose S. Sanchez, Ernelea P. Cao and Francis S. Magbanua................................... 8 Morphological characterization of wild Rhynchostylis gigantea in Thailand Surapong Anuttato Ratri Boonruangrod, Buppa Kongsamai and 20 Sermsiri Chanprame................................................................................................................ Current conditions and profitability of nutmeg industry in Bogor Regency, Indonesia Riskina Juwita and Shiro Tsuchida......................................................................................... 33 Socio-economic and environmental impacts of the conservation farming village program in upland communities in La Libertad, Negros Oriental Marilyn M. Elauria, Alessandro A. Manilay, Girlie Nora A. Abrigo, Simplicio M. Medina and Rosa B. De Los Reyes................................................................... 45 Population dynamics and growth pattern of the brown planthopper, Nilaparvata lugens (Stål) and its natural enemies in susceptible and resistant tropical rice varieties in central Thailand Laura Abril, Wantana Sriratanasak and Eiji Nawata.............................................................. 57 Serological and molecular detection of differential infections of bunchy top and mosaic causing viruses in tissue culture plantlets of abaca (Musa textilis Née) Filomena C. Sta. Cruz, Darwin M. Landicho, Emmanuel L. Bernardo and Evalour T. Aspuria .................................................................................................................................... 81 Survey of insect pests and diseases of Gabing San Fernando, Xanthosoma sagittifolium (L.) Schott & Melet in selected areas of Luzon and Zamboanga City, Philippines Bonifacio F. Cayabyab, Efren B. Villavicencio, Rene L. Limosinero, Virgilio T. Villancio, Jose Nestor M. Garcia......................................................................... 94 Chemical basis for repellency of Sargassum cinctum J. Agardh against Asian corn borer, Ostrinia furnacalis (Guenee) (Lepidoptera:Crambidae) Susan May F. Calumpang and Marcela M. Navasero........................................................... 103 Effect of organic amendments and microbial inoculant on nitrogen, phosphorus and potassium use efficiency of sugarcane under acid Typic Hapludand Ryan T. Sarimong, Pearl B. Sanchez, Rodrigo B. Badayos, Erlinda S. Paterno and Pompe C. Sta. Cruz........................................ .................................................................................... 114 Segregation pattern of resistance to soybean mosaic virus on Tanggamus x Taichung crossed population at F2:3 generation Nyimas Sadiyah, Alamanda Katartika Fahri,Hasriadi Mat Akin,Saiful Hikam and Maimun Barmawi........................................ ................................................................................ ......... 125 i J. ISSAAS Vol. 23, No. 2 (2017) Contributed Papers Page Combined effects of salinity, rice variety and rice growth stage on the diversity of bacterial communities associated with rice (Oryza sativa L.) Nolissa Delmo-Organo, Erlinda S. Paterno, Evelyn. F. Delfin, Glenn B. Gregorio, Nacita 134 B. Lantican, Jessica F. Simbahan........................................ ........................................ ........... Ultraviolet–B induced production of flavonoids in in vitro cultures of shallot (Allium cepa Var. Aggregatum G. Don CV Batanes) Geovannie Stanley S. Malab, Evalour T. Aspuria and Emmanuel L. Bernardo...................... 146 Political economic analysis of Indonesian rice market Sri Nuryanti, Dedi Budiman Hakim, Hermanto Siregar, and M. Husein Sawit....................... 158 Analysis of postharvest handling of table eggs produced in San Jose, Batangas, Philippines Abigail T. Lat and Julieta A. Delos Reyes......................................... ..................................... 169 Partial cloning and expression of ScBADH AND ScMIPS gene in wild and cultivated sugarcane under mimicking saline soil conditions Chanakan Laksana and Sontichai Chanprame……………………….…………………………. 182 Effects of aerenchyma formation on the root porosity, lateral root development, total water uptake and shoot biomass accumulation of global MAGIC rice under seedling-stage drought stress Vince Angelo G. Gicaraya, Nina M. Cadiz and Amelia Henry................................................ 192 Transmission of episomal Banana streak virus by mealybugs of different host plants Maria Luz J. Sison, Fe M. dela Cueva and Alora Pamela M. Pozon....................................... 203 Analysis of seasonality in monthly pork prices in the Philippines based on X-12 ARIMA Imelda R. Molina, Julieta A. Delos Reyes and Prudenciano U. Gordoncillo.......................... 215 Invited Paper Promoting Good Agricultural Practices (GAP) to enhance competitiveness, resilience and sustainability of smallhold saba/cardaba banana growers Edna A. Aguilar and H. A. Gabertan. ..................................................................................... 227 Reviewers ............................................................................................................................... 236 Members, Editorial Committee................................................................................................. 240 ii J. ISSAAS Vol. 23, No. 2: 1-7 (2017) EFFECT OF TRANS-2-HEXENAL VAPOR PRETREATMENT ON ALLEVIATTION OF HEAT SHOCK IN TOMATO SEEDLINGS (MICRO TOM) Naoki Terada, Atsushi Sanada, Hiroshi Gemma and Kaihei Koshio Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo Japan 156-8502 Corresponding author: [email protected] (Received: November 3, 2016; Accepted: July 10, 2017) ABSTRACT Trans-2-hexenal is a plant natural compound exhibiting a very safe, eco-friendly and strong antifungal activity. We investigated the effect of trans-2-hexenal in alleviating heat stress on tomato seedlings in trials conducted from November 28, 2016 to January 4, 2017 at the Tokyo University of Agriculture, Japan. Wilting in seedlings was alleviated through exposure to 0.001 or 0.010 ppm trans- 2-hexenal, compared to untreated seedlings at 48°C. On the other hand, seedlings treated with 1.000 or 10.000 ppm trans-2-hexenal showed severe wilting. Electrolyte leakage and malondialdehyde (MDA) levels of heat-treated tomato leaves also showed alleviation from heat stress upon exposure to 0.001 ppm or 0.010 ppm trans-2-hexenal vapor. Results suggest that pre-treatment of tomato seedlings with trans-2-hexenal protected tomato seedlings from stress due to heat shock, indicating a possible practical application in tropical developing countries, as a cheap, energy-saving and environment friendly technique. Key words: green leaf volatiles (GLVs), heat stress alleviation, natural compound INTRODUCTION Tomato, considered as one of the most important vegetables in the world, is conserved daily as a source of nutrients, like sugars, organic and amino acids, polyphenols, folic acid, lycopene (carotenoid), vitamin C, as well as minerals (Toor et al. 2005, Vinson et al. 2001 Giovanelli et al. 1999, Van Duyn and Pivonka, 2000). World tomato production has reached up to 170 million tons (FAOSTAT, 2014). In developing countries, there is positive correlation between vegetable consumption and price, and a negative correlation between vegetable price and morbidity rate (Bouis 1991). Accordingly, an insufficient vegetable supply might affect negatively human health, leading to increased infant mortality and abortion rate (Bouis 1991). In many Southeast Asian countries, productivity of main crops has increased recently in a drastic manner, shifting the focus of food problems from quantity to quality, such as the intake of micronutrients to promote health (Imai 1998). Thus, fresh vegetables, including tomato, attract much attention and a year round stable supply is desirable. In addition, a low cost and simple technique for tomato production under high temperature is particularly required. High temperature is one of the serious causes for decreased productivity of a variety of crops (Boyer 1982, Hall and Anthony 2001). To counteract the effects of increased temperature, agricultural equipment, such as air control facilities, are used. However, these require high investments for their introduction, and/or running costs (Hara 2016). Thus, there is a need for a simple and low-cost technology to alleviate the effects of stress due to high temperature. 1 Effect of trans-2-hexenal vapor pretreatment….. Recently, carbonyl compounds derived from peroxidized linolenic acid are recognized as important signals involved in the environmental stress response (Yamauchi et al. 2008, Mueller and Berger 2009). There is therefore a need for new agricultural technologies which could alleviate the environmental stress caused by such chemicals. Thus, we focused on the possibility of using trans-2- hexenal to alleviate the stress caused by heat on tomato. trans-2-Hexenal belongs