GUCY2F Protein Recombinant Human Protein Expressed in Sf9 Cells

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Catalog # Aliquot Size G12-31G-20 20 µg G12-31G-50 50 µg GUCY2F Protein Recombinant human protein expressed in sf9 cells Catalog # G12-31G Lot # U1858-3 Product Description Purity Recombinant human GUCY2F (367-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC156674. The purity of GUCY2F protein was determined to be >70% by Gene Aliases densitometry, GUCY2F approx. MW 110kDa. CYGF, GC-F, GUC2DL, GUC2F, RETGC-2, ROS-GC2 Formulation Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Storage and Stability Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background Guanylate cyclase 2F (GUCY2F) belongs to the adenylyl cyclase class-4/guanylyl cyclase family which also includes GUCY2D. Both GUCY2F and GUCY2D are responsible for the replenishment of cGMP in photoreceptors after exposure to light and are required for the normal kinetics of photoreceptor sensitivity and recovery, although disease mutations are restricted to GUCY2F Protein Full-length recombinant human protein expressed in sf9 cells GUCY2D. Catalog # G12-31G References Lot # U1858-3 1. Goraczniak R, et al: Structural and functional characteri- Purity >70% Concentration 0.05 µg/µl zation of a second subfamily member of the calcium- Stability 1yr at –70oC from date of shipment modulated bovine rod outer segment membrane Storage & Shipping Store product at –70oC. For optimal storage, guanylate cyclase, ROS-GC2. Biochem Biophys Res aliquot target into smaller quantities after Commun 234:666–670. 1997. centrifugation and store at recommended 2. Hunt DM, et al: Guanylate cyclases and associated temperature. For most favorable performance, activator proteins in retinal disease. Mol Cell Biochem. avoid repeated handling and multiple 334(1-2):157-68. 2010. freeze/thaw cycles. Product shipped on dry ice. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .

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