Supplementary Information S1

Candidate : examples of amino acid changes in notothenioids

Methods To evaluate notothenioid amino acid changes in a cross‐section of genes, six candidates from a range of functional categories of interest were chosen for a more detailed examination. The selected genes comprised small, well‐characterised genes present in multiple (mainly teleosts, but in some cases these analyses were extended to the when sequences from the spotted gar (Lepisosteus oculatus) were available) and available in at least two notothenioids. The amino acid composition of a series of six candidate genes were further analysed in depth at the amino acid sequence level. These genes were superoxide dismutase 1 (SOD1), neuroglobin, dihydrofolate reductase (both paralogues), p53 and calmodulin. Clustal X alignments were constructed from orthologues identified in the four Notothenioid transcriptomes along with other fish orthologues extracted from either SwissProt (Bateman et al., 2017) or Ensembl release 91 (Aken et al., 2017). Alignment data were visualised and annotated in BoxShade version 3.21 (https://embnet.vital‐ it.ch/software/BOX_form.html). Notothenioid‐specific changes were noted and the type of substitution in terms of amino acid properties was noted.

Results A range of amino acid changes were revealed, from virtually complete conservation between the notothenioids and other fish (calmodulin), to one amino acid substitution present in neuroglobin and SOD1, to more extensive changes in dihydrofolate reductase, dihydrofolate reductase‐like and p53. In the case of the latter three genes, notothenioid‐specific changes were at positions that were often highly variable in temperate fish species with up to six different amino acid substitutions and did not result in a definable pattern of directional substitution. In all cases, the notothenioid protein coding regions did not have consistent length differences compared to other fish.

Table showing amino acid substitutions in candidate proteins

Amino acid changes in Notothenioid candidate proteins compared with other fish. Colour code: blue: polar positive; red: polar negative; green: polar neutral; white: non‐polar aliphatic; purple: non‐polar aromatic; brown: small (proline and glycine); yellow: cysteine. Notes: ‘variable’ is recorded when substitutions in non‐Notothenioid fish have 2‐3 different amino acids with different properties in the same position; ‘no significant change’ is recorded where there is a change in amino acid in the notothenioids, which has the same physical properties as that of the non‐notothenioid fish species.

Amino Notothenioid acid Substitutions in other fish Notes substitution position Calmodulin No Notothenioid‐specific substitutions. 4 Notothenioid species‐specific substitutions

Superoxide dismutase 6 Notothenioid species‐specific substitutions 198 Leu Ile No significant change

Neuroglobin 3 Notothenioid species‐specific substitutions. Last 13 aa at 3’ end very different. 8 forms across 18 species. 85 Pro Thr No significant change

Dihydrofolate reductase 4 Notothenioid species‐specific substitutions. Notothenioid 3’ end slightly longer by 2‐6 aa 4 Met Val Pro Ile No significant change 20 Asp Asn His Lys Negative to neutral/positive 22 Gln Asn Asp No significant change 28 Val Ile Leu Lys No significant change 111 Thr Ala Val Polar neutral to non‐polar aliphatic 128 Gln Glu Gly Variable 129 Phe Ser Arg Ala Gly Variable 141 Met Leu No significant change 169 His Gln Glu Leu Val Variable

Dihydrofolate reductase‐like 12 Notothenioid species‐specific substitutions. First 10 aa highly variant 9 different forms in 11 species. Last 8 aa at 3’ end highly variant 9 forms in 11 species. 28 Glu Asp No significant change 30 Gly Gln Arg Thr Ile Variable 48 Thr Arg Gly Ala Ser Variable 55 Phe Met Val Lys Non‐polar to polar 78 Thr Ser Cys Val Ile Variable 85 Leu Lys Arg Thr Non‐polar aliphatic to polar 88 Asp Glu Phe Ser Thr Ile Gly Variable 90 Pro Val Ala No significant change 99 Ser Glu Gln Gly Lys His Variable 109 Gly Ala Ser Val Variable 110 Ser Gln Leu Glu Thr Variable 134 Leu Lys Glu Non‐polar aliphatic to polar 145 Lys Asp Asn Gln Positive to negative/neutral 181 Lys Asn Gln Positive to neutral 183 Val Ile No significant change

p53 11 Notothenioid species‐specific substitutions in core region. 5’ end highly variant with only 9 conserved residues in 93 aa. 3’ end highly variant with only 29 conserved residues in 81 aa. 107 Gln Asn Glu Lys Ala No significant change 109 Gln Arg His Glu Variable 113 Thr Ser No significant change 126 Gln Lys Ile Ser Variable 145 Gly Lys Ile Ser Variable 148 Ile Pro Non‐polar aliphatic to small 153 Ile Met Val No significant change 181 Ala Ser Thr Leu Variable 216 Leu Pro Arg Variable 223 Ile Met Phe No significant change 254 Ala Gln Pro Thr Variable 257 Leu Val Glu No significant change 264 Leu Phe Aliphatic to aromatic 268 Ile Val No significant change 299 Thr Ala Val Neutral to non‐polar aliphatic

References Aken BL, Achuthan P, Akanni W, Amode MR, Bernsdorff F, Bhai J, Billis K, Carvalho‐Silva D, Cummins C, Clapham P, et al. 2017. Ensembl 2017. Nucl Acids Res 45:D635‐D642.

Bateman A, Martin MJ, O'Donovan C, Magrane M, Alpi E, Antunes R, Bely B, Bingley M, Bonilla C, Britto R, et al. 2017. UniProt: the universal protein knowledgebase. Nucl Acids Res 45:D158‐D169.

Consensus line symbols: * = absolute conservation, . = up to 50% conservation across the alignment at that position. Dark squares are absolute conservation of amino acids. Light squares are conservative changes and white squares are divergent changes. species names are in blue, whilst red name denotes a temperate Notothenioid.

Calmodulin

DANRE 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG ELEEL 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG CTEID 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG ONCSP 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG TORCA 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG NEOIO 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG POEFO 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG ASTMX 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG TAKRU 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG ORYLA 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG XIPMA 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG PARCH 1 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG consensus 1 ************************************************************

DANRE 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE ELEEL 61 NGTIDFPEFLTMMAKKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE CTEID 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE ONCSP 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE TORCA 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE NEOIO 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE POEFO 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE ASTMX 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE TAKRU 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE ORYLA 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE XIPMA 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE PARCH 61 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDE consensus 61 **************.************************.********************

DANRE 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK ELEEL 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK CTEID 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK ONCSP 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK TORCA 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK NEOIO 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK POEFO 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK ASTMX 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK TAKRU 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK ORYLA 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK XIPMA 121 EVDEMIREADIDGDGQVNYEEFVQMMTAK PARCH 121 EVDEMIREADIDGDGQVNYEEFVTMMMSK consensus 121 ***********************.**..*

Superoxide dismutase 1 (SOD1)

XIPHE 1 MVLKAVCVLKGAGETTGTVHFEQEIESAPVKVTGEISGLTPGDHGFHVHAFGDNTNGCIS XIPMA 1 MVLKAVCVLKGAGETTGTVHFEQENESAPVKVTGEISGLTPGDHGFHVHAFGDNTNGCIS POEFO 1 MVLKAVCVLKGAGETTGTVHFEQENESAPVKVTGEIGGLTPGEHGFHVHAFGDNTNGCIS TAKRU 1 MAMKAVCVLKGAGDTSGTVYFEQENESAPVKLTGEIKGLTPGEHGFHVHAFGDNTNGCIS DANRE 1 MVNKAVCVLKGTGEVTGTVYFNQEGEKKPVKVTGEITGLTPGKHGFHVHAFGDNTNGCIS HYPMO 1 MVNKAVCVLKGDGQVTGTVYFEQEAEKSPVKLSGEITGLTAGKHGFHVHAFGDNTNGCIS SALSA 1 MALKAVCVLKGTGEVTGTVFFEQEGDGAPVKLTGEIAGLTPGEHGFHVHAFGDNTNGCMS ONCMY 1 MAMKAVCVLKGTGEVTGTVFFEQEGADGPVKLIGEISGLAPGEHGFHVHAYGDNTNGCMS GADMO 1 MVLKAVCVLKGTGDVTGTVFFEQEGDGAPVKLSGQIAGLAAGEHGFHVHVFGDNTNGCIS ASTMX 1 MVHKAVCVLKGTGEVTGTVFFEQVGDGAPVKVSGEITGLTPGLHGFHVHAFGDNTNGCIS CHIHA 1 ---KAVCVFKGAGEASGTVFFEQETDSCPVKLTGEIKGLTPGEHGFHVHAFGDNTNGCIS TREBE 1 ---KAVCVFKGTGEASGTVFFEQENDSAPVKLTGEIKGLTPGEHGFHVHAFGDNTNGCIS NEOIO 1 MVIKAVCVLKGAGEASGTVFFEQENDSSPVKLTGEIKGLTPGEHGFHVHAFGDNTNGCIS GASAC 1 MVVKAVCVLKGAGETTGTIYFEQESDKAAVKLTGEIKGLTPGEHGFHVHAFGDNTNGCIS LEPOC 1 MVLKAVCVLKGSGEVSGTVHFEQQNGDAPVKVTGKISGLTPGDHGFHVHAFGDNTNGCVS ORYLA 1 MVLKAVCVLKGTGETNGVVNFEQESDSAPVKVTGEIKGLTPGKHGFHIHVYGDNTNGCVS ORENI 1 MVLKAVCVLKGTGDTSGTVYFEQENESAPVKLTGEIKGLTPGEHGFHVHAFGDNTNGCIS consensus 1 ...*****.**.*. .*...*.*. ....**..*.* **..*.****.*..*******.*

XIPHE 61 AGPHYNPFTKNHGGPTDVERHVGDLGNVTAGADNIAKIDIKDTFIKLSGPNSIIGRTMVI XIPMA 61 AGPHYNPFTKNHGGPTDVERHVGDLGNVTAGADNIAKIDIKDTFIKLSGPNSIIGRTMVI POEFO 61 AGPHYNPFSKKHGGPTDEERHVGDLGNVTAGADNIAKIDIKDSFIKLSGPNSIIGRTMVI TAKRU 61 AGPHYNPHNKTHAGPTDADRHLGDLGNVTAGADNIAKIDIKDSMLTLTGPYSIIGRTMVI DANRE 61 AGPHFNPHDKTHGGPTDSVRHVGDLGNVTADASGVAKIEIEDAMLTLSGQHSIIGRTMVI HYPMO 61 AGPHFNPYSKNHGGPTDSERHVGDLGNVTAGENGVAKIDIVDKMLTLSGPDSIIGRTMVI SALSA 61 AGPHFNPHNHTHGGPTDTVRHVGDLGNVTAAADSVAKINIQDEILSLAGPHSIIGRTMVI ONCMY 61 AGPHFNPHNQTHGGPTDAVRHVGDLGNVTAGADNVAKINIQDKMLTLTGPDSIIGRTMVI GADMO 61 AGPHFNPHKKDHAGPNDVDRHVGDLGNVTAGADKVAKIDITDKMLSLNGPFSIVGRTMVI ASTMX 61 AGPHYNPHNKTHGGPTDEIRHVGDLGNVTAGADGVAKINIEDKMLSLSGPHSIVGRTMVI CHIHA 58 AGPHFNPHNKTHAGPTDENRHVGDLGNVTAAADNVAKLDITDKMITLAGQYSIIGRTMVI TREBE 58 AGPHFNPHNKTHAGPTDEDRHVGDLGNVTAAADNVAKLNITDKMITLAGQYSIIGRTMVI NEOIO 61 AGPHFNPHNKTHAGPTDENRHVGDLGNVTAAADNVAKLDITDKMITLAGQYSIIGRTMVI GASAC 61 AGPHFNPHSKTHAGPNDEIRHVGDLGNVTAGGDNIAKIDITDKVITLTGQHSIIGRTMVI LEPOC 61 AGPHYNPHNKTHGGPRDENRHVGDLGNVTAGEDKVANINIEDKHITLTGQFSIIGRTMVI ORYLA 61 AGPHFNPYNKNHGGPEDAERHVGDLGNVTAGDNNVAKIDITDKLIRLSGPDSIVGRTVVV ORENI 61 AGPHFNPYNKNHGGPKDAERHVGDLGNVTAADN-VAKIEITDKVITLTGPDSIIGRTMVI consensus 61 ****.**....*.**.* .**.********.....*...* *....*.*. **.***.*.

XIPHE 121 HEKADDLGKGGNEESLKTGNAGGRLACGVIGITQ------XIPMA 121 HEKADDLGKGGNEESLKTGNAGGRLACGVIGIAQ------POEFO 121 HEKADDLGKGGNEESLKTGNAGGRLACGVIGITQ------TAKRU 121 HEKADDLGKGGNEESLKTGNAGGRLACGVIGITQ------DANRE 121 HEKEDDLGKGGNEESLKTGNAGGRLACGVIGITQ------HYPMO 121 HEKEDDLGKGNNEESLKTGNAGGRLACGVIGIAQ------SALSA 121 HEKADDLGKGDNEESRKTGNAGSRLACGVIGIAQ------ONCMY 121 HEKADDLGKGGNEESLKTGNAGGRQACGVIGIAQ------GADMO 121 HEKADDLGKGGNEESLKTGNAGSRLACGVIGI------ASTMX 121 HEKEDDLGKGGDEESLKTGNAGARLACGVIGIAQ------CHIHA 118 HEKADDLGKGGNDESLKTGNAGGRLACGVIGIAQMDRRPT TREBE 118 HEKADDLGKGGNDESLKTGNAGGRLACGVIGIAQ------NEOIO 121 HEKADDLGKGGNDESLKTGNAGGRLACGVIGIAQ------GASAC 121 HEKADDLGKGGNEESLKTGNAGSRLACGVIGIAQ------LEPOC 121 HEKADDLGKGGDDESLKTGNAGGRLACGVIGIAQ------ORYLA 121 HEKVDDLGKGGNDESLKTGNAGARLACGVIGIAQ------ORENI 120 HEKVDDLGKGGNEESLKTGNAGGRLACGVIGITQ------consensus 121 ***.******...**.******.*.*******..

Neuroglobin

DANRE 1 MEKLSEKDKGLIRDSWESLGKNKVPHGIVLFTRLFELDPALLTLFSYSTNCGDAPECLSS ASTMX 1 MEKLTGKDKELIRDSWESLGKNKVPHGIVMFTRLFELDPSLLTLFNYKTNCGVVPECLSS POEFO 1 MGELSVKDKELIRGSWESLGKNKVPHGVIMFSRLFELDPALLNLFHYSTNCDSKQDCLSS XIPMA 1 MGELSVKDKELIRGSWESLGKNKVPHGVIMFSRLFELDPALLNLFHYSTNCDSKQDCLSS ORYLA 1 MEKLSGKDKELIRGSWESLGKNKVPHGVIMFSRLFELDPALLSLFNYNTNCGSTQDCLSS TAKRU 1 MEKLSSKDKELIRGSWDSLGKNKVPHGVIMFSRLFELDPELLSLFHYTTNCGSTQDCLSS TETNG 1 MEKLSSKDKELIRGSWDSLGKNKVPHGVILFSRLFELDPELLNLFHYTTNCGSTQDCLSS PARCH 1 ------PSEGE 1 ------VFVCGRLFELDPELLTLFHYTTNCGSTQDCLSS CHAAC 1 MEKLSEKDKELIRGSWESLGKNKVPHGVVMFSRLFELDPELLTLFHYTTNCGSTQDCLSS DISMA 1 MEKLSEKDKELIRGSWESLGKNKVPHGVVMFSRLFELDPELLTLFHYTTNCGSTQDCLSS CHIMY 1 MEKLSGKDKELIRGSWESLGKNKVPHGVVMFSRLFELDPELLTLFHYTTNCGSTQDCLSS NEOIO 1 MEKLSGKDKELIRGSWESLGKNKVPHGVVMFSRLFELDPELLTLFHYTTNCGSTQDCLSS GYMAC 1 MEKLSGKDKELIRGSWESLGKNKVPHGVVMFSRLFELDPELLTLFHYTTNCGSTQDCLSS BOVVA 1 MEKLSGKDKELIRGSWESLGKNKVPHGVVMFSRLFELDPELLTLFHYTTNCGSTQDCLSS ONCMY1 1 MEKLTEKEKELIRVSWESLGKDKVPHGVIMFSRLFELEPALLNLFHYNTNCGTIQDCLSS ONCMY2 1 MEKLTEKDKELIRGSWESLGKNKVPHGVVMFSRLFELEPALLNLFHYNTNCSPTQDCLSS LEPOC 1 MEKLTAKDKEMIRDSWERLGKNKLTHGTIMFTRLFELDPELLGLFHYNTPYSSPQECLSS consensus 1 ......

DANRE 61 PEFLEHVTKVMLVIDAAVSHLDDLHTLEDFLLNLGRKHQAVGVNTQSFALVGESLLYMLQ ASTMX 61 PEFLEHVTKVMLVVDAAVSHLDDLHTLEDFLLNLGKKHQAVGVNTQSFALVGESLLYMLQ POEFO 61 PEFLDHVTKVMLVIDAAVSHLDDLHSLEDFLLNLGRKHQAVGVSTQSFTEVGESLLYMLQ XIPMA 61 PEFLDHVTKVMLVIDAAVSHLDDLHSLEDFLLNLGRKHQAVGVSTQSFTVVGESLLYMLQ ORYLA 61 PEFLDHVTKVMLVIDAAVNHLDDLHSLEDFLLNLGRKHQAVGVSTQSFAVVGESLLYMLQ TAKRU 61 PEFLEHVTKVMLVIDAAVSHLDDLHSLEDFLLNLGRKHQAVGVNPQSFATVGESLLYMLQ TETNG 61 PEFLEHVTKVMLVIDAAVSHLDDLHSLEDFLLNLGRKHQAVGVKPQSFAMVGESLLYMLQ PARCH 1 ------VMLVIDAAVSHLDDLPSLEDFLLNLGRKHQAVGVNTQSFAEVGESLLYMLQ PSEGE 34 PEFLEHVTKVMLVIDAAVSHLDDLPSLEDFLLNLGRKHQAVGVNTQSFAEVGESLLYMLQ CHAAC 61 PEFLEHVTKVMLVIDAAVSHLDDLPSLEDFLLNLGRKHQAVGVNTQSFAEVGESLLYMLQ DISMA 61 PEFLEHVTKVMLVIDAAVSNLDDLPSLEDFLLNLGRKHQAVGVNTQSFAEVGESLLHMLQ CHIMY 61 PEFLEHVTKVMLVIDAAVSHLDDLPSLEDFLLNLGRKHQAVGVNTQSFAEVGESLLYMLQ NEOIO 61 PEFLEHVTKVMLVIDAAVSHLDDLPSLEDFLLNLGRKHQAVGVNTQSFAEVGESLLYMLQ GYMAC 61 PEFLEHVTKVMLVIDAAVSHLDDLPSLEDFLLNLGRKHQAVGVNTQSFAEVGESLLYMLQ BOVVA 61 PEFLEHVTKVMLVIDAAVSNLDDLPSLEDFLLNLGGKHQAVGVNTQSFAEVGESLLYMLQ ONCMY1 61 PEFLDHVTKVMLVIDAAVSHLDNLHTLEDFLLNLGKKHQAVGVNTQSFAVVGESLLYMLQ ONCMY2 61 PEFLDHVTKVMLVIDAAVSHLDDLHTLEDFLLNLGKKHQAVGVNTQSFAVVGESLLYMLQ LEPOC 61 PEFVDHINKVMLVVDAAVTHLDNLHSLEEYLVNLGRKHQAVGVQTQSFAAVGESLLYMLE consensus 61 ...... ****.****..**.*..**..*.***.*******..***..******.**.

DANRE 121 SSLGPAYTTSLRQAWLTMYSIVVSAMTRGWAKNGEHKSN ASTMX 121 CSLGTAYTTALRQAWLNMYTIVVSAMTRGWAKNGEHKSN POEFO 121 CSLGQAYTAPLRQAWLNMYSIVVAVMSRGWAKNGEDKAD XIPMA 121 CSLGQAYTAPLRQAWLNMYTIVVAVMSRGWSKNGEDKAD ORYLA 121 CSLGQAYTAALSQAWLNMYSIVVAAMSRGWAKNGEDKAD TAKRU 121 CSLGQAYTASLRQAWLNMYSVVVAAMSRGWAKNGEDKAD TETNG 121 CSLGQAYTASLRQAWLNMYSVVVASMSRGWAKNGEDKAD PARCH 52 CSLGQAYTAPLRQAWLNLYSIVVAAMSQGWAKNGEDKAD PSEGE 94 CSLGQAYTAPLRQAWLNLYSIVVAAMSQGWAKNGEDKAD CHAAC 121 CSLGQAYTAPLRQAWLNLYSIVVAAMSQGWAKNGEDKAD DISMA 121 CSLGQAYTAPLRQAWLNLYSIVVAAMSQGWAKNGEDKAD CHIMY 121 CSLGQAYTAPLRQAWLNLYSIVVAAMMPRLG------NEOIO 121 CSLGQAYTAPLRQAWLNLYSIVVAAMMPRLG------GYMAC 121 CSLGQAYTAPLRQAWLNLYSIVVAAMMPRLG------BOVVA 121 CSLGQAYTAPLRQAWLNLYSIVVAAMSRGWA------ONCMY1 121 CSLGQGYTAPLRQAWLNMYTIVVAAMSRGWAKNGEHKTD ONCMY2 121 CSLGHGYTGPLRQAWLNMYTIVVAAMSRGWAKNGEHKTD LEPOC 121 RSLGPAYTSTLRHAWLTLYSVVVEAMSSGWTKNGKSQTD consensus 121 .***..**..*..***..*..**..*......

Dihydrofolate reductase

TAKRU 1 MSRVVNAIVAVCPDLGIGMNGDLPWHPIRLNKEFVHFRKMTSTPSVNGKQNVVIMGRKTW TETNI 1 MARVLNAIVAVCPDLGIGRNGDLPWHPIRLDNEFKHFRKMTSTPSVNGKQNVVIMGRKTW HARAN 1 MSRMLNAIVAVCPDLGIGMDGQLPWHPVRLNNEFKHFRKITATPSVEGKQNVVIMGRKTW NEOIO 1 MSRMLNAIVAVCPDLGIGMDGQLPWHPVRLNNEFKHFRKMTATPSVEGKQNVVIMGRKTW GASAC 1 MSRVLNGIVAVCPDLGIGCHGNLPWHPLRLSNEFKHFRRMTATASVKDKQNVVIMGRKTW ORENI 1 MPRVLNAIVAVCPDRGIGNKGNLPWHPIRLSKEFAHFRKMTATPSVKGKQNVVIMGKKTW LEPOC 1 MPRPINCIVAVCPNMGIGHNGNLPWHPKRLSNEFKYFQKMTMTPTLEGQQNAVIMGRKTW DANRE 1 MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTW consensus 1 *.*..*.******..*** .*.*****.**..*...*...*.*...... *.****.***

TAKRU 61 FSIPERNRPLANRINIVLSRRCREPPAGAHHLARDLPSALRLVDS-ELAEQADQVWVIGG TETNI 61 FSIPEKHRPLANRINIVLSRRSREPPAGAHHLAHDLSSALQLVET-QLADR-DQVWVIGG HARAN 61 FSIPEKNRPLNNRINIVLSRECRAPPAGAHHLAPDFNSALRLVET-ELAERTDQVWVIGG NEOIO 61 FSIPEKNRPLNNRINIVLSRECRAPPAGAHHLAPDFSSALRLVET-ELAERTAQVWVIGG GASAC 61 FSIPEKNRPLNNRINIVLSRELKAPPAGAHHLAPDFSSALRLVDT-ELAEQADQVWVIGG ORENI 61 YSIPEKNRPLSNRINIVLSRECKVPPAGAHYLASDFSSALRLIDT-ELADQADQVWVIGG LEPOC 61 FSIPERNRPLKNRINIVLSRELKNPPEGAHYLASDFSSALRLLDSAPQTDQVDQVWVIGG DANRE 61 FSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGG consensus 61 .***...*** *********... .*.***.** *..***.*...... ***.***

TAKRU 120 SSLYQELMEGTGTTRLFVTRILKQFECDTFLPEINPARYRRLPQFPGVAQELQEEEGIQY TETNI 119 SSLYQEMMERPGTSRLFVTHVLKQFDCDTFLPEIRPQRYRLLPQFPGVPQELQEEKGIQY HARAN 120 SSLYKELMQFPGTRRLFITRIMKQFECDTFLPEISLDKYRLLPQFPGVPHELQEENGIQY NEOIO 120 SSLYKELMQFPGTRRLFVTRIMKQFECDTFLPEISLDRYRLLPQFPGVPHELQEENGIQY GASAC 120 SSLYKELLESPGTRRLFVTRILKQFECDTFLPEICPDRYRLLPEFPGVPEELQEENGVQY ORENI 120 SSLYKEMMGSTGMRRLFVTQILKQFECDTFLPEISLDKYRLLPEFPDVPQELQEENGIQY LEPOC 121 SSVYKEAMEAPGWCRLFVTQILQDFECDTFLPEINLNKYRLLPEFPGVPLEVQEENGIRY DANRE 121 SSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQY consensus 121 **.*.*... .*..***.*.....*.****.*.. ...*..** **.*. ..**..*..*

TAKRU 180 RFQVYESIEH------TETNI 179 RYQVYESLE------HARAN 180 RFEVYESIDELC---- NEOIO 180 RFEVYESIDELCNLNK GASAC 180 RFEVYESIDNK----- ORENI 180 RFKVYESIQE------LEPOC 181 KFEVYESIQN------DANRE 181 VFEVYESIEH------consensus 181 ...****..

Dihydrofolate reductase‐like

HARAN 1 MENTQNKVQRKPVRLIAAACNDMGIGKEGGMPWSLPSEFQWFLNKVTTVSRPGKFNMMVW PARCH 1 MENTQNKVQRKPVRIIAAACNDMGIGKEGGMPWSLPSEFQWFLNKVTTVSRPGKFNMMVW NEOIO 1 ------SEFQSFLNRVTTVSRPGKFNMMVW GASAC 1 --MSGDNVQKKPVRLIAAACSGGGIGKDGQMPWDLPSEFRYFRNHVQGVSRPGKMNMMVW TAKRU 1 --MEAAKGNKKPVRVIAAVCNNRGIGKDNQMPWSIPAEFQYFLNTVTRVSRPGNMNMMVW TETNG 1 --MEPAKVIKKPVRLIAAKCNGGGIGKDNRMPWSLPSEFRFFLSTITRVSRPGKMNMIIW POEFO 1 MEKDREKVQKKPVRLIAATCNNMGMGKDGTLPWSLPSEFQYFLNTITRVSRPGNMNLLIW XIPMA 1 MEQDRETVRKKPVQLIAAICNNMGMGKDGTLPWSLPSEFQYFLNTITRVSRPGNMNLLIW ORYLA 1 MEN-RLEERRKPVRLIAAVCRNNGIGKDGTLPWSLPSEFQYFLNTITRVSRPGNVNLMIW DANRE 1 MMNIEGEEMRKPIRLIAAACRDMGIGKDGQIPWCLPKEFQFLLDTITAVSAPGKKNLIVW LEPOC 1 MSDCEGKVHRKSIRLIAAACNGMGIGKDGILPWSLPKEFKFFLDTITSVSSPDKKNLLIW consensus 1 ...... **...... **.*...*...*

HARAN 61 GKKSWFNHPESTFPLPNTLHAVLSLTLDSPPDHAHFVGSDLEAAVRLAGSPPLADLIETI PARCH 61 GKKCWFSHPESTFPLPNTLHAVLSLTLDSPPDHAHFVCSDLEAAVRLAGSPPLADLIETI NEOIO 25 GKTCWFSHPESTFPLPNTFHAVLSLTLDSPPDHAHFVCSDLEAAVCLAGSPPLADLIETI GASAC 59 GKLCWYSVPKPDFPLPNVLHVVLSKTLESVPDHAHFLCEDLDAAARLAVQPPLADLIETI TAKRU 59 GKQCWVSHPDSTFPLPNILHAVLSKTLFTVPDHAHFLCESLDAAVRLASEPPLADLIEII TETNG 59 GKQCWISHPESTFPLPNVLHTVLSTTLFAVPDHAHFVCETLDAAVRLASEPPLADLIEIV POEFO 61 GRLCWNSHPENIFPLANSLHVVLSKTLSSVPDHAHFLCQDFESAVRLAAQPPLSGIIETV XIPMA 61 GRLCWNSHSENMFPLANSLHVVLSKTLSSAPDHAHFLCQDFESAVRLAAQPPLSDIIETV ORYLA 60 GRLCWFSHPDDLFPLPNSLHVVLSKTLTSVPNHAQFLCGDFESAVRLAALPPLADIIETI DANRE 61 GRICWFSCPETVFPLANCINLVLSRKMISVPPHAHYLCKDFDSIIRLVSEPPLCHTVEVI LEPOC 61 GKRCWISFPESLHPLANCIHVVLSRTMGCVPDHAHYLCHDLPSVIQLGSTHPLSDKIETI consensus 61 *. .*...... **.*....***... ..*.**...... *.. .**....*..

HARAN 121 WIVGGMQVYKEALLHQWCDLVYLTKVMADFDCDVFFPEFDRELFKLQEGFPGVPSEIQEE PARCH 121 WIVGGVQVYKEALLHRWCDLVYLTKVMADFDCDVFFPKFDRELFKLQEG------NEOIO 85 WIVGGVQVYKEALLHRWCDLVYLTKVMADFDCDVFFPEFDRELFKLQEGFPGVPTEIQEE GASAC 119 WVVGGTQVYEVALKHPWCDLVYLTDVMADFDCNVFFPEFDRGLFKVQEGFPGVPSEIQEE TAKRU 119 WIVGGVQVYKEAMEHPRCDLIYLTDIMAEFECDVFFPEFDKKLFEVQDSFPDVPNGIQED TETNG 119 WIVGGVQVYKEAMEHPWCDLIYLTDIMAEFECDVFFPEFDRQLFQVQDGFPDVPDGIQEE POEFO 121 WILGGTQVYEDALKHPWCDLLYLTDVMADFDCDVFFPDFDRELFKLQETFPDVPIEIQEE XIPMA 121 WILGGTQVYEDALKHPWCDLLYLTDVMADFDCDVFFPEFDRELFKLQEKFPDVPSEIQEE ORYLA 120 WVLGGTKVYEEALKHPCCDLLYFTDVMADFDCDVFFPDFDRELFRVQEEFPDVPSEVQEE DANRE 121 WILGGTEVYKESLEHPWCDLIYLTNIMANFECDVFFPEFDPNIFRKQKSFPGVPDEIIEE LEPOC 121 WILGGAELYKESLKHPWCDYIYLTQVMADFDCDTFFPEFDREIYKLQDEFPGVPSEIQED consensus 121 *..** ..*.... *..**..*.*..**.*.*..***.**...... *......

HARAN 181 KGVKYQFQVFKRETGDNV- PARCH ------NEOIO 145 KGVKYQFQVFKRETGNKV- GASAC 179 NGIKYKFQVFKKETGDAV- TAKRU 179 NGIKYKCQVYKRKTAVCLL TETNG 179 NGIKYKCQVYKRETAAEDQ POEFO 181 NGIRFKCQVFKKKTDDTM- XIPMA 181 NGIRFKCQVFKKKTDDAF- ORYLA 180 NGIKFKCQVFKRVTMEDHL DANRE 181 NGIKFQFQVFKKIKN---- LEPOC 181 QGIKLKFQVFKKDMH---- consensus 181 ......

P53

HARAN 1 MEEQSLDDLTLSQTMPLSQDSFSELWNTVSAPLFSYLTPTVINVPEETWKTDGHMDMLLL NEOIO 1 MEEQSLDDLTLSQTMPLSQDSFKELWNTVSAPLFSYLTPTVN-VPEETWKTDGNMDMLLL TAKRU 1 MEDE------GFSLPLSQDTFQDLWENVDVCMHP--DSPVS------QLMNYP TETNG 1 MEEE------TFSLPLSQDTFQDLWENVAAPSISTIQTTVSGNECW---QDGSLTMALM POEFO 1 -MET------NFPLPLSQDTFHELWNTVVLSTENESLATGD------GLL XIPMA 1 MEEA------DLTLPLSQDTFHDLWNNVFLSTENESLPPPE------GLL consensus 1 ...... *****.*..**. * ......

HARAN 61 NDHSLNEVFDEKLFELPPPDMSTMDGVNPTSSTVPVTSDYPGEYGFQLQFQKTGTAKSVT NEOIO 60 NDHSLTEVFDEELFELPPPDMSTMDGVNPTSSTVPVTSDYPGEYGFQLQFQKTGTAKSVT TAKRU 40 ELP-----FNEELFNLP-SEMASKDSANLSTPTVPVTTDYPGEYGFELRFQKSGTAKSVT TETNG 51 DMP-----YDEDLFNLP-SELPNKDGANSSCPTVPVTTDHPGEYDFKLRFQKSGTAKSVT POEFO 38 DLN------MDFWENGELPQQETKNV-PAAPMVPAISNYAGELDFALHFNDSGTAKSVT XIPMA 39 SQN------MDFWEDPET-MQETKNV-PTAPTVPAISNYAGEHGFNLEFNDSGTAKSVT consensus 61 ...... **...... **..*.*.*...*******

HARAN 121 STYSEQLNKLYCQLAKTTPVEVLFGKEIPLGAIIRATAVYKKTEHVAEVVRRCPHHQNED NEOIO 120 STYSEQLNKLYCQLAKTTPVEVLLGKEIPLGAIIRATAVYKKTEHVAEVVRRCPHHQNED TAKRU 94 STYSEILNKLYCQLAKTSLVEVLLIKKPPAGAVLRATAIYKKIEHVADVVRRCPHHQNED TETNG 105 STYSESLNKLYCQLAKTSPLEVLLSREPPLGAMLRATAIYKKTEHVAEVVRRCPHHQNED POEFO 90 STFSEKLTKLFCQLAKTTPIGILVKVEPPQGAVIRATAVYKKTEHVAEVVKRCPHHQSED XIPMA 90 STYSVKLGKLFCQLAKTTPIGVLVKEEPPQGAVIRATAVYKKTEHVGEVVKRCPHHQSED consensus 121 **.*. *.**.******.....*. ...*.**..****.***.***..**.******.**

HARAN 181 AADHRSHLIRVEGSQRAQYFEDPNTKRQSVTVPYELPQLGSEITTILLSFMCNSSCMGGM NEOIO 180 AADHRSHLIRVEGSQRALYFEDTNTKRQSVTVPYELPQLGSEITTILLSFMCNSSCMGGM TAKRU 154 SAAHRSHLIRMEGSQRAQYFEDPHTKRQSVTVPYEPPQLGSEFTTILLSFMCNSSCMGGM TETNG 165 STENRSHLIRMEGSQRAQYFEDPHTKRQSVTVPYEPPQLGSEFTTILLSFMCNSSCMGGM POEFO 150 TSDNKSHLIRVEGSQLAQYFEDPNTKRQSVTVPYERPQRGSEMTTILLSFMCNSSCMGGM XIPMA 150 LSDNKSHLIRVEGSQLAQYFEDPNTRRHSVTVPYERPQLGSEMTTILLSFMCNSSCMGGM consensus 181 ... .*****.****.*.****..*.*.******* **.***.*****************

HARAN 241 NRRPILTILTLETAEGLVLGRRCLEVRICACPGRDRKTEEENSTKVQSGTKQTKKRKSTP NEOIO 240 NRRPILTILTLETAEGLVLGRRCLEVRICACPGRDRKTEEENSTKMQSGTKQTKKRKSTP TAKRU 214 NRRPILTILTLETQEGVVLGRRCFEVRVCACPGRDRKTEEANSTNMQNGTKETKKRKSVP TETNG 225 NRRPILAILTLETQEGVVLGRRCFEVRVCACPGRDRKTEEANSTKMQTETKDAKKRKSAP POEFO 210 NRRPILTILTLETPEGEVLGRRCFEVRVCACPGRDRKTEEEN--LEKNGTKQTKKRKSAP XIPMA 210 NRRPILTILTLETTEGEVLGRRCFEVRVCACPGRDRKTEEGN--LEKSGTKQTKKRKSAP consensus 241 ******.****** **.******.***.************.*...... **..*****.*

HARAN 301 ---GSSSMKKSRPTSSAEEEDKDVFVLNVRGHERYEMLKKINDGLELLDKDRKPETRVKH NEOIO 300 ---GSSSMKKSRPTSSAEEEDKDVFVLNVRGRERYEMLKKINDGLELLDKDRKPETRVKH TAKRU 274 PPAAAAAAKKSKTASSAEEDDKELFTLQIRGRKRYEMLKKINDGLELLENKPKCKAAAKP TETNG 285 -TSDSTTVKKSRTASSAEEDDKEVFTLQIRGRKRYEMIKRINDGLDLLENKTKSKTTYKP POEFO 268 -APDTSTAKKSKSVSSGEDEDKELYTLQIRGRERFLMFKKLNDGLELMEKMG---PKKKQ XIPMA 268 -APDTSTAKKSKSASSGEDEDKEIYTLSIRGRNRYLWFKSLNDGLELMDKTG---PKIKQ consensus 301 .....***...**.*..**....*..**..*....*..****.*...... *

HARAN 358 EFALPSSGKRLLHRGDKSDSD NEOIO 357 EFALPSSGKRLLHKGDKSDSD TAKRU 334 ECPVPPRGKRLLHRGEKSDSD TETNG 344 EGPVLPSGKRLMHRGEKSDSD POEFO 324 EVPAPSSGKRLLKGG--SDSD XIPMA 324 EIPAPSSGKRLLKGG--SDSD consensus 361 * .....****...*..****

Accession numbers for fish species used in ClustalW alignments of candidate proteins

Abbreviation Species name Common Calmodulin DHFR DHFR‐like Neuroglobin p53 SOD1 name DANRE Danio rerio Zebrafish Q6PI52 A2BGQ1 F1QRU7 Q90YJ2 O73872 POEFO Poecilia formosa Amazon A0A087XTZ7 A0A087YOQ3 A0A087YDQ5 A0A087XP53 A0A087YGV3 molly ASTMX Astyanax mexicanus Blind cave W5L960 W5K4Z1 W5KMP4 fish TAKFU Takifugu rubripes Fugu H2TXN3 H2SSG1 H286U1 H2VBJ2 H2U135 H2T728 (pufferfish) ORYLA Oryzias latipes Medaka I6L4R5 H2MP65 H2MNQ2 H2LHN5

XIPMA Xiphophorus Platyfish M3ZHJ6 M4AXY9 M4AL53 Q9W679 M4AT47 maculatus LEPOC Lepistosteus Spotted gar W5MD25 W5NDT1 W5MYS7 W5MSO0 oculatus TETNG Tetraodon Green H3CG21 H3CSF7 Q90W04 H3CXQ0 nigroviridis spotted pufferfish ORENI Orechromis niloticus Nile tilapia I3JW88 I3JAL8

GADMO Gadus morhua Cod ENSGMOP 00000016337

GASAC Gasterosteus Stickleback ENSGACG ENSGACG ENSAGP aculeatus 00000003734 00000013483 00000027216

ELEEL Electrophorus Electric eel P02594 electricus

CTEID Ctenopharyngodon Grass carp Q6IT78 idella ONCSP Oncorhynchus sp. Salmon P62156 species TORCA Torpedo californica Pacific P62151 electric ray XIPHE Xiphophorus helleri Green D9JOD6 swordtail HYPMO Hypophthalmichthys Silver carp D9MPI5 molitrix

SALSA Salmo salar Atlantic Q3ZLR1 salmon ONCMY Oncorhynchus Rainbow P59742, Q8QHI0 mykiss trout P59743

Temperate Notothenioid BOVVA variegatus Thornfish D2EA92

Antarctic Notothenioids HARAN Harpagifer Plunderfish This study This study This study antarcticus PARCH Parachaenichthys Dragonfish This study This study This study charcoti

NEOIO Neopagetopsis Jonah's This study This study This study D2EA95 This study This study ionah icefish PSEGE Pseudochaenichthys South This study georgianus Georgia icefish CHIHA Crocodile Q3ZLR2 hamatus icefish TREBE Trematomus emerald Q3ZLR3 bernacchi rockcod

CHAAC Chaenocephalus blackfin P86880 aceratus icefish

DISMA Dissostichus Antarctic P86881 mawsoni toothfish CHIMY Chionodraco myseri Myers' D2EA94 icefish GYMAC Gymnodraco ploughfish D2EA93 acuticeps

Amino-acid usage at non-synonymous positions

20000 vertebrate Notothenioids

15000

10000 Total counts

5000

0

E D H K R C G N Q STY A F I L M P V W Supplementary Figure S2: STRING output showing protein‐protein interactions for the duplicated Notothenioid genes

Supplementary Table S1: Number of raw reads produced for the different tissues from each of the four Notothenioids. In the cases where more than one was sampled, fish numbers 1‐2 of P. charcoti correspond to the original fish IDs of Pcha2 and Pcha3, whilst fish numbers 1‐4 of P. georgianus correspond to fish Ids Pgeo4, Pgeo7, Pgeo8 and Pgeo42013 respectively.

Species Libraries Sample Fish number number 1 2 3 4 Neopagetopsis ionah spleen n=1 14297063 Harpagifer Brain n=1 12546421 antarcticus White n=1 13377648 muscle Liver n=1 14256208 Kidney n=1 17626116 Skin n=1 11757830 heart n=1 20170567 Parachaenichtys Brain n=2 15799167 16587667 charcoti Ventricle n=2 16583013 23591777 Pectoral n=2 14510454 16817765 muscle White n=2 14104635 18283472 muscle Liver n=2 15994258 12042469 Spleen n=2 19810431 15772144 Ovary n=1 11340950 Testis n=1 14027425 Head kidney n=2 20526312 13890077 Trunk n=2 12090753 14346721 kidney Whole n=2 20190257 12797609 blood Intestine n=1 18137550 Gill n=1 14866598 Red blood n=1 16877920 cells Pseudochaenichtys Brain n=4 18331125 21409188 13818468 11886875 georgianus Ventricle n=3 39915788 11910723 18100509 Pectoral n=3 15339621 19639537 16514463 muscle White n=3 16341220 18684208 8351606 muscle Liver n=3 32503701 21546986 31493263 Spleen n=3 14895003 14613838 17384436 Ovary n=2 15012348 15856491 Testis n=1 11474215 Head kidney n=3 12843904 19601598 13214987 Trunk n=3 14928781 17621020 19966982 kidney Supplementary Table S2: Duplicated genes in pgeo and pcha. Manually verified with annotations from e! Ensembl Gasterosteus aculeatus (stickleback Broad S1) release 84

Stickleback ID PGEO PCHA ID GENE NAME

Multiple gene family members 5 copies ENSGACT00000000161 Pgeo_c52773_g2_i1 Pcha_c4262_g1_i1 ENSGACT00000000161 Pgeo_c62292_g1_i1 Pcha_c17043_g1_i1 ENSGACT00000000161 Pgeo_c63600_g2_i1 Pcha_c46345_g1_i1 ENSGACT00000000161 Pgeo_c63874_g2_i1 Pcha_c33405_g1_i1 ENSGACT00000000161 Pgeo_c63948_g1_i2 Pcha_c46941_g1_i4 ENSGACT00000000161 Pgeo_c63825_g1_i1 Pcha_c46991_g1_i2 ENSGACT00000000161 Pgeo_c63847_g1_i1 Pcha_c42821_g1_i1 si:ch211‐167j9.4 pol‐like protein ENSGACT00000021683 Pgeo_c59245_g2_i1 Pcha_c39519_g5_i1 ENSGACT00000021683 Pgeo_c42953_g3_i1 Pcha_c24907_g1_i1 ENSGACT00000021683 Pgeo_c42953_g2_i1 Pcha_c39519_g4_i1 ENSGACT00000021683 Pgeo_c56697_g2_i1 Pcha_c45214_g2_i2 ENSGACT00000021683 Pgeo_c42953_g1_i1 Pcha_c39519_g3_i1 tln2a‐201 talin 2a

4 copies ENSGACT00000010024 Pgeo_c60741_g1_i1 Pcha_c2169_g2_i1 ENSGACT00000010024 Pgeo_c60741_g2_i1 Pcha_c37010_g1_i1 ENSGACT00000010024 Pgeo_c98473_g1_i1 Pcha_c91291_g1_i1 ENSGACT00000010024 Pgeo_c10033_g2_i1 Pcha_c135897_g1_i1 ENSGACT00000010024 Pgeo_c10033_g1_i1 Pcha_c99812_g1_i1 lrp1bb‐201 low density lipoprotein receptor related protein 1Bb ENSGACT00000012119 Pgeo_c29311_g1_i1 Pcha_c5057_g2_i1 ENSGACT00000012119 Pgeo_c56004_g1_i1 Pcha_c43193_g3_i4 ENSGACT00000012119 Pgeo_c35078_g2_i1 Pcha_c41387_g2_i1 ENSGACT00000012119 Pgeo_c35078_g1_i1 Pcha_c41387_g1_i1 ENSGACT00000012119 Pgeo_c11710_g1_i1 Pcha_c78990_g1_i1 prkg1a‐201 cGMP‐dependent type 1a ENSGACT00000023710 Pgeo_c40675_g1_i1 Pcha_c37430_g1_i1 ENSGACT00000023710 Pgeo_c62496_g2_i1 Pcha_c17612_g1_i1 ENSGACT00000023710 Pgeo_c63360_g1_i1 Pcha_c34556_g1_i1 ENSGACT00000023710 Pgeo_c63834_g1_i1 Pcha_c46978_g1_i3 pcdh2g12 protocadherin 2 gamma 12

3 copies ENSGACT00000004996 Pgeo_c22925_g2_i1 Pcha_c128126_g1_i1 ENSGACT00000004996 Pgeo_c36888_g3_i1 Pcha_c94800_g1_i1 ENSGACT00000004996 Pgeo_c22925_g1_i1 Pcha_c93869_g1_i1 ENSGACT00000004996 Pgeo_c36888_g1_i1 Pcha_c126526_g1_i1 si:ch73‐194h10.2‐201 myosin VIIB ENSGACT00000009267 Pgeo_c62170_g2_i1 Pcha_c42559_g4_i1 ENSGACT00000009267 Pgeo_c54450_g1_i1 Pcha_c38365_g2_i2 ENSGACT00000009267 Pgeo_c59352_g1_i1 Pcha_c30920_g1_i1 prkd3‐201 protein kinase D3 ENSGACT00000009315 Pgeo_c44658_g1_i1 Pcha_c13048_g1_i1 ENSGACT00000009315 Pgeo_c44658_g4_i1 Pcha_c7364_g1_i1 ENSGACT00000009315 Pgeo_c44658_g3_i1 Pcha_c86559_g1_i1 smurf2 (1‐to‐many)‐201 SMAD specific E3 ubiquitin protein ligase 2 ENSGACT00000011412 Pgeo_c62545_g2_i1 Pcha_c44880_g13_i2 ENSGACT00000011412 Pgeo_c61546_g8_i1 Pcha_c46544_g4_i1 ENSGACT00000011412 Pgeo_c62545_g3_i1 Pcha_c44880_g8_i1 mast3b‐201 microtubule associated serine/threonine kinase 3b ENSGACT00000011682 Pgeo_c45418_g1_i1 Pcha_c46506_g5_i1 ENSGACT00000011682 Pgeo_c51799_g2_i1 Pcha_c34173_g1_i2 ENSGACT00000011682 Pgeo_c62455_g4_i1 Pcha_c34173_g2_i1 st3gal1‐201 ST3 beta‐galactoside alpha‐2,3 sialyltransferase 1 ENSGACT00000024004 Pgeo_c14505_g1_i1 Pcha_c24524_g1_i1 ENSGACT00000024004 Pgeo_c59408_g1_i1 Pcha_c36507_g2_i1 ENSGACT00000024004 Pgeo_c58084_g1_i1 Pcha_c39388_g1_i1 ENSGACT00000024004 Pgeo_c973_g1_i1 Pcha_c36507_g1_i1 cel (1‐to‐many)‐201 carboxyl ester lipase

2 copies ENSGACT00000000110 Pgeo_c58819_g1_i1 Pcha_c38454_g1_i2 ENSGACT00000000110 Pgeo_c31700_g1_i1 Pcha_c46199_g4_i2 creld1 (1‐to‐many)‐201 cysteine rich with EGF‐like domains 1 ENSGACT00000000392 Pgeo_c57739_g1_i1 Pcha_c26221_g1_i2 ENSGACT00000000392 Pgeo_c22337_g1_i1 Pcha_c39534_g2_i2 zeb2a‐201 zinc finger E box binding homeobox 2a ENSGACT00000000694 Pgeo_c55044_g1_i1 Pcha_c5227_g1_i1 ENSGACT00000000694 Pgeo_c37361_g1_i1 Pcha_c27044_g2_i1 si:dkey‐222b8.1‐201 SMAD family member ENSGACT00000000774 Pgeo_c14461_g1_i1 Pcha_c44531_g12_i1 ENSGACT00000000774 Pgeo_c57605_g2_i1 Pcha_c46705_g6_i2 spryd3 (1‐to‐many)‐201 spry domain containing 3 ENSGACT00000000909 Pgeo_c61559_g3_i1 Pcha_c41953_g1_i1 ENSGACT00000000909 Pgeo_c52032_g1_i1 Pcha_c43458_g2_i4 c7orf43 (1‐to‐many)‐201 uncharacterised ENSGACT00000001053 Pgeo_c63071_g2_i1 Pcha_c10483_g1_i1 ENSGACT00000001053 Pgeo_c61974_g14_i1 Pcha_c46798_g8_i1 ENSGACT00000001053 Pgeo_c61974_g10_i1 Pcha_c46798_g9_i1 ENSGACT00000001053 Pgeo_c61974_g16_i1 Pcha_c46798_g5_i2 neb‐201 nebulin ENSGACT00000001056 Pgeo_c63071_g1_i1 Pcha_c46798_g11_i1 ENSGACT00000001056 Pgeo_c50534_g1_i1 Pcha_c46798_g2_i2 neb‐202 nebulin ENSGACT00000001160 Pgeo_c52874_g1_i1 Pcha_c40052_g1_i2 ENSGACT00000001160 Pgeo_c37648_g1_i1 Pcha_c33791_g1_i1 tex264a‐201 testis expressed 264a ENSGACT00000001182 Pgeo_c49351_g1_i1 Pcha_c974_g1_i1 ENSGACT00000001182 Pgeo_c49913_g1_i1 Pcha_c61115_g1_i1 slc1a1‐201 solute carrier family 1 (glutamate) ENSGACT00000001674 Pgeo_c62372_g10_i1 Pcha_c39879_g1_i2 ENSGACT00000001674 Pgeo_c14556_g1_i1 Pcha_c36360_g1_i3 sema4ab‐201 semaphorin 4b precursor family ENSGACT00000002016 Pgeo_c61255_g1_i1 Pcha_c43760_g1_i1 ENSGACT00000002016 Pgeo_c59553_g1_i1 Pcha_c43411_g2_i1 cacna2d2b‐201 calcium channel voltage dependent alpha 2 delta subunit 2b ENSGACT00000002116 Pgeo_c61845_g1_i1 Pcha_c30114_g1_i1 ENSGACT00000002116 Pgeo_c32213_g1_i1 Pcha_c29400_g1_i1 col6a3‐201 collagen type VI alpha 3 ENSGACT00000002247 Pgeo_c52632_g1_i1 Pcha_c35338_g1_i1 ENSGACT00000002247 Pgeo_c60925_g1_i1 Pcha_c40617_g1_i2 pard3‐201 opar3 family cell polarity regulator ENSGACT00000002310 Pgeo_c53326_g3_i1 Pcha_c30537_g3_i1 ENSGACT00000002310 Pgeo_c59471_g1_i1 Pcha_c43918_g2_i6 ccny (1‐to‐many)‐201 Y ENSGACT00000002646 Pgeo_c62117_g2_i1 Pcha_c45650_g1_i3 ENSGACT00000002646 Pgeo_c62117_g3_i1 Pcha_c45650_g2_i1 si:ch211‐173p18.3‐201 uncharacterised ENSGACT00000002657 Pgeo_c6212_g1_i1 Pcha_c32328_g3_i1 ENSGACT00000002657 Pgeo_c45412_g2_i1 Pcha_c32328_g2_i2 ntrk3a‐202 neurotrophic tyrosine kinase receptor type 3a ENSGACT00000002827 Pgeo_c12716_g1_i1 Pcha_c46502_g5_i6 ENSGACT00000002827 Pgeo_c50642_g1_i1 Pcha_c46502_g8_i1 tmem163a‐201 transmembrane protein 163 ENSGACT00000003085 Pgeo_c53899_g1_i1 Pcha_c46199_g10_i2 ENSGACT00000003085 Pgeo_c56247_g1_i1 Pcha_c46199_g5_i1 ENSGACT00000003085 Pgeo_c62830_g1_i1 Pcha_c46685_g1_i2 si:ch211‐219a4.3‐201 poly(ADP‐ribose) polymerase family member 14 ENSGACT00000003427 Pgeo_c40129_g1_i1 Pcha_c21433_g1_i1 ENSGACT00000003427 Pgeo_c50879_g1_i1 Pcha_c38730_g2_i1 nkain4‐201 Na+/K+ transporting ATPase interacting 4 ENSGACT00000003499 Pgeo_c47906_g1_i1 Pcha_c12418_g2_i1 ENSGACT00000003499 Pgeo_c55276_g5_i1 Pcha_c20200_g1_i2 pcbp4‐201 poly(rC) binding protein 4 ENSGACT00000003723 Pgeo_c13625_g1_i1 Pcha_c41174_g1_i5 ENSGACT00000003723 Pgeo_c52972_g1_i1 Pcha_c41964_g1_i1 ppt2‐201 palmitoyl protein thioesterase 2 ENSGACT00000004466 Pgeo_c63589_g1_i1 Pcha_c46598_g4_i1 ENSGACT00000004466 Pgeo_c63607_g3_i1 Pcha_c46267_g2_i1 si:dkey‐79i2.4 (1‐to‐many)‐201 uncharacterised ENSGACT00000004680 Pgeo_c38781_g1_i1 Pcha_c7383_g1_i1 ENSGACT00000004680 Pgeo_c41733_g1_i1 Pcha_c46095_g2_i1 zgc:101000‐202 deoxyribonuclease precursor ENSGACT00000004695 Pgeo_c56226_g1_i1 Pcha_c27758_g2_i1 ENSGACT00000004695 Pgeo_c56226_g3_i1 Pcha_c27758_g1_i1 pabpc1a‐201 poly(A) binding protein cytoplasmic 1a ENSGACT00000004707 Pgeo_c49225_g1_i1 Pcha_c26779_g1_i2 ENSGACT00000004707 Pgeo_c61800_g1_i1 Pcha_c44872_g1_i6 si:ch211‐272n13.3‐201 ankyrin repeat domain 30B ENSGACT00000004730 Pgeo_c58772_g1_i1 Pcha_c43004_g1_i1 ENSGACT00000004730 Pgeo_c61585_g1_i1 Pcha_c37054_g5_i1 crnp1b‐201 cysteine‐serine rich nuclear protein 1b ENSGACT00000004883 Pgeo_c8432_g1_i1 Pcha_c28496_g1_i1 ENSGACT00000004883 Pgeo_c35088_g1_i1 Pcha_c30251_g1_i1 arl4d‐201 ADP ribosylation factor‐like 4D ENSGACT00000005803 Pgeo_c60392_g2_i1 Pcha_c45804_g8_i4 ENSGACT00000005803 Pgeo_c60392_g3_i1 Pcha_c46512_g1_i9 arhgef28‐201 rho guanine nucleotide exchange factor 28 ENSGACT00000005969 Pgeo_c47764_g1_i1 Pcha_c39545_g2_i4 ENSGACT00000005969 Pgeo_c55942_g2_i1 Pcha_c36891_g1_i1 cdk17 (1‐to‐many)‐201 cyclin‐dependent kinase 17 ENSGACT00000006199 Pgeo_c37852_g1_i1 Pcha_c38901_g2_i1 ENSGACT00000006199 Pgeo_c18437_g1_i1 Pcha_c63219_g1_i1 si:ch1073‐406l10.2‐201 uncharacterised ENSGACT00000006261 Pgeo_c53664_g1_i1 Pcha_c12901_g1_i2 ENSGACT00000006261 Pgeo_c50018_g2_i1 Pcha_c23564_g3_i1 ap2m1 (1‐to‐many)‐201 adaptor related protein complex 2 mu 1 subunit ENSGACT00000006381 Pgeo_c56057_g1_i1 Pcha_c42687_g1_i1 ENSGACT00000006381 Pgeo_c61989_g1_i1 Pcha_c45381_g1_i2 tdrd6‐201 tudor domain containing 6 ENSGACT00000006412 Pgeo_c13981_g1_i1 Pcha_c26939_g1_i1 ENSGACT00000006412 Pgeo_c32262_g1_i1 Pcha_c33732_g1_i3 igfbp3‐201 insulin‐like growth factor binding protein 3 ENSGACT00000006484 Pgeo_c42226_g1_i1 Pcha_c3904_g1_i1 ENSGACT00000006484 Pgeo_c57883_g2_i1 Pcha_c40250_g2_i2 megf11‐201 multiple EGF‐like domains 11 ENSGACT00000006538 Pgeo_c54821_g1_i1 Pcha_c44746_g6_i1 ENSGACT00000006538 Pgeo_c22569_g1_i1 Pcha_c4472_g1_i1 si:dkey‐33c12.4‐201 tetratricopeptide repeat domain 31 ENSGACT00000007013 Pgeo_c47927_g3_i1 Pcha_c26945_g1_i1 ENSGACT00000007013 Pgeo_c47927_g2_i1 Pcha_c32480_g1_i1 zbtb17‐202 zinc finger and BTB domain containing 17 ENSGACT00000007083 Pgeo_c41841_g1_i1 Pcha_c46638_g3_i3 ENSGACT00000007083 Pgeo_c58009_g1_i1 Pcha_c29578_g1_i1 fam57a‐201 family with sequence similarity 57 member A ENSGACT00000007279 Pgeo_c56915_g1_i1 Pcha_c16463_g1_i1 ENSGACT00000007279 Pgeo_c68650_g1_i1 Pcha_c63118_g1_i1 fign‐201 fidgetin ENSGACT00000007744 Pgeo_c94897_g1_i1 Pcha_c76771_g1_i1 ENSGACT00000007744 Pgeo_c8150_g1_i1 Pcha_c126710_g1_i1 nr4a2a‐201 nuclear receptor subfamily 4 group A member 2a ENSGACT00000007781 Pgeo_c19740_g1_i1 Pcha_c33866_g1_i1 ENSGACT00000007781 Pgeo_c32558_g1_i1 Pcha_c42385_g1_i1 poc1bl‐201 POC1 centriolar protein homologue B ENSGACT00000007821 Pgeo_c29346_g1_i1 Pcha_c47251_g1_i1 ENSGACT00000007821 Pgeo_c34883_g1_i1 Pcha_c27317_g1_i1 dnase1‐202 deoxyribonuclease 1 ENSGACT00000007983 Pgeo_c54400_g2_i1 Pcha_c36464_g2_i2 ENSGACT00000007983 Pgeo_c36711_g2_i1 Pcha_c39074_g2_i1 lhfpl2b lipoma HMGIC fusion partner‐like 2b ENSGACT00000008307 Pgeo_c54372_g2_i1 Pcha_c44828_g2_i1 ENSGACT00000008307 Pgeo_c58401_g1_i1 Pcha_c44828_g3_i1 ext1a‐202 exostosin glycosyltransferase 1a ENSGACT00000008308 Pgeo_c54149_g1_i1 Pcha_c40864_g1_i1 ENSGACT00000008308 Pgeo_c33623_g1_i1 Pcha_c33254_g1_i1 stac3‐201 SH3 and cysteine rich domain 3 ENSGACT00000008524 Pgeo_c32483_g1_i1 Pcha_c31375_g1_i1 ENSGACT00000008524 Pgeo_c48159_g2_i1 Pcha_c32650_g2_i1 shmt‐201 serine hydroxylmethyl transferase 2 (mitochondrial) ENSGACT00000008629 Pgeo_c62486_g5_i1 Pcha_c121800_g1_i1 ENSGACT00000008629 Pgeo_c62486_g6_i1 Pcha_c44621_g1_i3 si:dkey224e22.2‐201 beta galactosidase precursor ENSGACT00000008799 Pgeo_c54312_g1_i1 Pcha_c44795_g1_i1 ENSGACT00000008799 Pgeo_c48703_g1_i1 Pcha_c33997_g2_i1 clmp‐201 CXADR‐like protein ENSGACT00000008807 Pgeo_c48349_g1_i1 Pcha_c42933_g1_i1 ENSGACT00000008807 Pgeo_c44113_g1_i1 Pcha_c32463_g1_i1 e2f3‐201 e2f transcription factor 3 ENSGACT00000008814 Pgeo_c61797_g2_i1 Pcha_c63859_g1_i1 ENSGACT00000008814 Pgeo_c61797_g1_i1 Pcha_c44770_g4_i1 atrnl1b‐201 attractin‐like 1b ENSGACT00000009022 Pgeo_c61120_g3_i1 Pcha_c38875_g1_i3 ENSGACT00000009022 Pgeo_c69225_g1_i1 Pcha_c51718_g1_i1 cd109‐201 CD109 molecule ENSGACT00000009069 Pgeo_c22613_g1_i1 Pcha_c62164_g1_i1 ENSGACT00000009069 Pgeo_c39030_g1_i1 Pcha_c39184_g2_i1 cx28.9‐201 connexin 28.9 ENSGACT00000009172 Pgeo_c19454_g1_i1 Pcha_c32691_g1_i1 ENSGACT00000009172 Pgeo_c59922_g1_i1 Pcha_c46057_g1_i8 tiam2a‐201 T cell lymphoma invasion and metastasis 2a ENSGACT00000009653 Pgeo_c48242_g1_i1 Pcha_c28090_g1_i2 ENSGACT00000009653 Pgeo_c10993_g1_i1 Pcha_c23367_g1_i2 senp5 (1‐to‐many)‐201 SUMO1/sentrin‐specific peptidase 5 ENSGACT00000009656 Pgeo_c54190_g1_i1 Pcha_c44775_g2_i2 ENSGACT00000009656 Pgeo_c62194_g3_i1 Pcha_c42273_g2_i1 ap3b2‐201 adaptor‐related protein complex 3 beta 2 subunit ENSGACT00000009905 Pgeo_c46129_g1_i1 Pcha_c33874_g1_i1 ENSGACT00000009905 Pgeo_c61516_g1_i1 Pcha_c122027_g1_i1 novel gene ataxin 2 family member ENSGACT00000010010 Pgeo_c57566_g1_i1 Pcha_c45509_g2_i1 ENSGACT00000010010 Pgeo_c58453_g2_i1 Pcha_c45509_g3_i1 jag2b‐201 jagged 2b ENSGACT00000010015 Pgeo_c61014_g5_i1 Pcha_c46476_g3_i1 ENSGACT00000010015 Pgeo_c57290_g1_i1 Pcha_c38062_g1_i1 prox2‐201 prospero homeobox 2 ENSGACT00000010057 Pgeo_c7839_g1_i1 Pcha_c30121_g2_i1 ENSGACT00000010057 Pgeo_c59055_g6_i1 Pcha_c38608_g3_i1 nkx2‐3‐201 NK2 homeobox 3 ENSGACT00000010138 Pgeo_c13935_g1_i1 Pcha_c40738_g1_i1 ENSGACT00000010138 Pgeo_c54471_g1_i1 Pcha_c37323_g1_i1 zg:.123010‐202 tetratricopeptide repeat 31 family member ENSGACT00000010156 Pgeo_c45137_g1_i1 Pcha_c11647_g1_i1 ENSGACT00000010156 Pgeo_c45137_g2_i1 Pcha_c46210_g3_i1 kif21b‐201 kinesin family member 21B ENSGACT00000010159 Pgeo_c54696_g2_i1 Pcha_c41106_g1_i1 ENSGACT00000010159 Pgeo_c58180_g3_i1 Pcha_c44921_g3_i5 ptprb‐201 protein tyrosine phosphatase receptor type b ENSGACT00000010192 Pgeo_c63052_g8_i1 Pcha_c46381_g10_i1 ENSGACT00000010192 Pgeo_c63052_g4_i1 Pcha_c46381_g1_i1 srpk1b‐201 SRSF protein kinase 1b ENSGACT00000010219 Pgeo_c50331_g1_i1 Pcha_c31218_g2_i1 ENSGACT00000010219 Pgeo_c50331_g3_i1 Pcha_c31218_g1_i1 ap3b2‐201 adenosine monophosphate deaminase 2b ENSGACT00000010242 Pgeo_c59731_g6_i1 Pcha_c16021_g1_i2 ENSGACT00000010242 Pgeo_c58632_g1_i1 Pcha_c38359_g1_i1 ENSGACT00000010242 Pgeo_c59731_g1_i1 Pcha_c34950_g1_i2 mapk14a‐202 mitogen activated protein kinase 14a ENSGACT00000010276 Pgeo_c58945_g2_i1 Pcha_c32861_g1_i1 ENSGACT00000010276 Pgeo_c50729_g1_i1 Pcha_c43952_g1_i1 si:dkey‐219c3.2‐201 transcription initiation factor TFIID family member ENSGACT00000010296 Pgeo_c53190_g1_i1 Pcha_c46856_g1_i1 ENSGACT00000010296 Pgeo_c725_g1_i1 Pcha_c46518_g1_i6 fcgbp (1‐to‐many)‐201 Fc fragment of IgG binding protein ENSGACT00000010443 Pgeo_c87380_g1_i1 Pcha_c17540_g1_i1 ENSGACT00000010443 Pgeo_c36409_g1_i1 Pcha_c41523_g2_i2 pon1‐202 paraoxonase 1 ENSGACT00000010534 Pgeo_c7052_g1_i1 Pcha_c46828_g2_i1 ENSGACT00000010534 Pgeo_c59603_g1_i1 Pcha_c46185_g1_i3 novel gene uncharacterised ENSGACT00000010745 Pgeo_c34955_g1_i1 Pcha_c34887_g1_i1 ENSGACT00000010745 Pgeo_c57224_g1_i1 Pcha_c34887_g2_i1 zgc:195001 (1‐to‐many)‐201 uncharacterised ENSGACT00000010903 Pgeo_c53479_g1_i1 Pcha_c45870_g1_i1 ENSGACT00000010903 Pgeo_c60164_g1_i1 Pcha_c42665_g1_i1 racgap1‐201 rac GTPase activating protein 1 ENSGACT00000011135 Pgeo_c61797_g8_i1 Pcha_c16433_g1_i1 ENSGACT00000011135 Pgeo_c42780_g1_i1 Pcha_c29874_g1_i1 crp1‐201 C reactive protein 1 ENSGACT00000011216 Pgeo_c62745_g1_i1 Pcha_c45681_g6_i1 ENSGACT00000011216 Pgeo_c62193_g2_i1 Pcha_c45643_g2_i19 scn8a (1‐to‐many) sodium channel voltage gated type VIII alpha subunit ENSGACT00000011579 Pgeo_c65427_g1_i1 Pcha_c101932_g1_i1 ENSGACT00000011579 Pgeo_c61093_g3_i1 Pcha_c39013_g1_i2 slco1c1‐201 solute carrier organic anion transporter family member 1c1 ENSGACT00000011859 Pgeo_c54907_g2_i1 Pcha_c35753_g1_i1 ENSGACT00000011859 Pgeo_c54907_g1_i1 Pcha_c41985_g2_i1 asap2a‐201 ArfGAP with SH3 domain ankyrin repeat and PH domain 2a ENSGACT00000011906 Pgeo_c57831_g1_i1 Pcha_c34464_g1_i2 ENSGACT00000011906 Pgeo_c61586_g2_i1 Pcha_c44851_g2_i1 arhgef25 (1‐to‐many)‐201 Rho guanine nucleotide exchange factor 25 ENSGACT00000012164 Pgeo_c62428_g1_i1 Pcha_c46268_g3_i3 ENSGACT00000012164 Pgeo_c57984_g2_i1 Pcha_c36787_g1_i2 dgkh‐201 diacylglycerol kinase eta ENSGACT00000012203 Pgeo_c57887_g1_i1 Pcha_c47270_g1_i1 ENSGACT00000012203 Pgeo_c56981_g1_i1 Pcha_c32939_g2_i2 tex2l‐201 testis expressed 2 like ENSGACT00000012260 Pgeo_c53777_g2_i1 Pcha_c26708_g1_i4 ENSGACT00000012260 Pgeo_c46499_g1_i1 Pcha_c42517_g2_i1 ptena‐201 phosphatase and tensin homologue A ENSGACT00000012277 Pgeo_c66287_g1_i1 Pcha_c34490_g1_i1 ENSGACT00000012277 Pgeo_c29004_g1_i1 Pcha_c39752_g1_i1 rlf‐201 rearranged L‐myc fusion ENSGACT00000012395 Pgeo_c37766_g1_i1 Pcha_c43441_g1_i1 ENSGACT00000012395 Pgeo_c51864_g1_i1 Pcha_c44077_g1_i3 calcoco1a calcium binding and coiled coiled domain 1a ENSGACT00000012437 Pgeo_c49812_g1_i1 Pcha_c40712_g1_i2 ENSGACT00000012437 Pgeo_c54433_g1_i1 Pcha_c32647_g1_i2 si:ch211‐207n2.8‐201 sortilin‐related VPS10 domain containing receptor 3 ENSGACT00000012690 Pgeo_c7378_g1_i1 Pcha_c30691_g2_i1 ENSGACT00000012690 Pgeo_c61266_g1_i1 Pcha_c33731_g1_i1 ENSGACT00000012690 Pgeo_c50660_g1_i1 Pcha_c14135_g1_i1 novel gene inter alpha trypsin inhibitor heavy chain H3 precursor ENSGACT00000013094 Pgeo_c57618_g1_i1 Pcha_c36661_g1_i1 ENSGACT00000013094 Pgeo_c8013_g1_i1 Pcha_c29601_g2_i1 tspan18b‐201 tetraspanin 18b ENSGACT00000013153 Pgeo_c56849_g1_i1 Pcha_c104039_g1_i1 ENSGACT00000013153 Pgeo_c47731_g2_i1 Pcha_c41984_g1_i1 mpp2b‐201 membrane protein palmitoylated 2b ENSGACT00000013368 Pgeo_c59800_g1_i1 Pcha_c34248_g1_i1 ENSGACT00000013368 Pgeo_c14232_g1_i1 Pcha_c21465_g1_i2 ola1‐201 obg‐like ATPase 1 ENSGACT00000013464 Pgeo_c59498_g4_i1 Pcha_c107989_g1_i1 ENSGACT00000013464 Pgeo_c57244_g1_i1 Pcha_c31385_g1_i1 top1l‐201 topoisomerase (DNA) 1 like ENSGACT00000013519 Pgeo_c60354_g3_i1 Pcha_c39103_g1_i1 ENSGACT00000013519 Pgeo_c12174_g1_i1 Pcha_c13662_g1_i1 gata1a‐201 gata binding protein 1a ENSGACT00000013694 Pgeo_c58988_g1_i1 Pcha_c122467_g1_i1 ENSGACT00000013694 Pgeo_c58988_g2_i1 Pcha_c45248_g13_i2 ENSGACT00000013694 Pgeo_c58988_g3_i1 Pcha_c45248_g12_i2 ENSGACT00000013694 Pgeo_c58988_g6_i1 Pcha_c81127_g1_i1 ENSGACT00000013694 Pgeo_c58988_g7_i1 Pcha_c10102_g1_i1 ENSGACT00000013694 Pgeo_c55641_g3_i1 Pcha_c44633_g1_i3 ENSGACT00000013694 Pgeo_c58988_g4_i1 Pcha_c72745_g1_i1 ENSGACT00000013694 Pgeo_c58988_g5_i1 Pcha_c23894_g1_i1 fbn2b‐201 fibrillin 2b ENSGACT00000013902 Pgeo_c13645_g1_i1 Pcha_c20825_g1_i1 ENSGACT00000013902 Pgeo_c39971_g2_i1 Pcha_c20787_g1_i1 cox6b2‐201 cytochrome c oxidase VIb polypeptide 2 ENSGACT00000014150 Pgeo_c59913_g1_i1 Pcha_c37634_g1_i3 ENSGACT00000014150 Pgeo_c60980_g6_i1 Pcha_c90557_g1_i1 roraa‐201 RAR‐related orphan receptor A paralogue a ENSGACT00000014397 Pgeo_c86474_g1_i1 Pcha_c38842_g1_i1 ENSGACT00000014397 Pgeo_c63191_g1_i1 Pcha_c40929_g1_i1 txnrd1‐201 thioredoxin reductase 1 ENSGACT00000014497 Pgeo_c58550_g1_i1 Pcha_c20324_g1_i1 ENSGACT00000014497 Pgeo_c50728_g2_i1 Pcha_c33187_g1_i1 map2k6‐202 mitogen activated protein kinase kinase 6 ENSGACT00000014683 Pgeo_c54915_g1_i1 Pcha_c39538_g1_i2 ENSGACT00000014683 Pgeo_c55175_g1_i1 Pcha_c42540_g2_i3 nfatc2‐201 nuclear factor of activated T cells cytoplasmic dependent 2 ENSGACT00000014706 Pgeo_c61137_g1_i1 Pcha_c45622_g2_i1 ENSGACT00000014706 Pgeo_c48279_g1_i1 Pcha_c39624_g1_i1 tmc6a‐201 transmembrane channel‐like 6a ENSGACT00000014996 Pgeo_c46825_g1_i1 Pcha_c44389_g1_i1 ENSGACT00000014996 Pgeo_c51300_g1_i1 Pcha_c51163_g1_i1 glis2‐201 GLIS family zinc finger 2 ENSGACT00000015282 Pgeo_c32703_g3_i1 Pcha_c42098_g4_i1 ENSGACT00000015282 Pgeo_c32703_g1_i1 Pcha_c42098_g1_i1 si:dkey‐283b15.4‐202 cytohesin 2 ENSGACT00000015509 Pgeo_c53123_g1_i1 Pcha_c37829_g1_i1 ENSGACT00000015509 Pgeo_c63546_g1_i1 Pcha_c35300_g1_i1 novel gene sterile alpha motif domain containing 9 family member ENSGACT00000015602 Pgeo_c63563_g2_i1 Pcha_c7753_g1_i1 ENSGACT00000015602 Pgeo_c31842_g1_i1 Pcha_c39232_g1_i2 ENSGACT00000015602 Pgeo_c48260_g1_i1 Pcha_c35603_g1_i1 grk5l‐201 G‐protein coupled receptor 5 like ENSGACT00000015636 Pgeo_c48574_g3_i1 Pcha_c109458_g1_i1 ENSGACT00000015636 Pgeo_c62471_g12_i1 Pcha_c23278_g2_i1 scrt2‐201 scratch family zinc finger 2 ENSGACT00000015746 Pgeo_c58900_g1_i1 Pcha_c41102_g1_i1 ENSGACT00000015746 Pgeo_c34961_g1_i1 Pcha_c49909_g1_i1 h63st6‐201 heparan sulphate glucosamine 3 sulphotransferase 6 ENSGACT00000015748 Pgeo_c50480_g3_i1 Pcha_c45570_g6_i6 ENSGACT00000015748 Pgeo_c56187_g2_i1 Pcha_c45874_g1_i3 ENSGACT00000015748 Pgeo_c50480_g2_i1 Pcha_c45570_g12_i1 kdm5bb‐201 lysine(K)‐specific demethylase 5Bb ENSGACT00000015868 Pgeo_c40628_g1_i1 Pcha_c42155_g1_i1 ENSGACT00000015868 Pgeo_c62042_g4_i1 Pcha_c26018_g1_i2 b3gnt2b‐201 UDP‐GlcNAc:betaGal beta‐1,3‐N‐acetylglucosaminyl transferase 2b ENSGACT00000015902 Pgeo_c54886_g1_i1 Pcha_c39116_g1_i3 ENSGACT00000015902 Pgeo_c60951_g2_i1 Pcha_c46255_g6_i1 trim9‐201 tripartite motif containing 9 ENSGACT00000016323 Pgeo_c60228_g2_i1 Pcha_c44205_g4_i1 ENSGACT00000016323 Pgeo_c60228_g3_i1 Pcha_c40925_g2_i2 abhd17a (1‐to‐many)‐201 abhydrolase domain containing 17a ENSGACT00000016464 Pgeo_c60743_g2_i1 Pcha_c590_g1_i1 ENSGACT00000016464 Pgeo_c62959_g15_i1 Pcha_c23596_g1_i1 kctd9a‐201 potassium channel tetramerization domain containing 9a ENSGACT00000016703 Pgeo_c48775_g2_i1 Pcha_c37412_g2_i1 ENSGACT00000016703 Pgeo_c34901_g1_i1 Pcha_c37412_g1_i1 aldh6a1‐202 aldehyde dehydrogenase 6 family member A1 ENSGACT00000016722 Pgeo_c53952_g1_i1 Pcha_c41962_g1_i2 ENSGACT00000016722 Pgeo_c53077_g2_i1 Pcha_c33049_g2_i2 ENSGACT00000016722 Pgeo_c53077_g1_i1 Pcha_c33049_g1_i1 gtpbp1‐201 GTP binding protein 1 ENSGACT00000016888 Pgeo_c55619_g1_i1 Pcha_c46884_g1_i11 ENSGACT00000016888 Pgeo_c61335_g1_i1 Pcha_c49816_g1_i1 nacc1 (1‐to‐many)‐201 nucleus accumbens associated 1 BEN and BTB (POZ) domain containing ENSGACT00000017010 Pgeo_c61857_g2_i1 Pcha_c42627_g5_i2 ENSGACT00000017010 Pgeo_c61857_g1_i1 Pcha_c42627_g1_i1 mdga2‐201 MAM domain containing glycosylphosphatidylinositol anchor 2 ENSGACT00000017426 Pgeo_c61477_g4_i1 Pcha_c33760_g2_i1 ENSGACT00000017426 Pgeo_c61477_g1_i1 Pcha_c33760_g1_i1 si:dkey‐20i10.4‐201 gamma aminobutyric acid (GABA) A receptor beta 1 ENSGACT00000017559 Pgeo_c62086_g11_i1 Pcha_c25447_g3_i1 ENSGACT00000017559 Pgeo_c40387_g1_i1 Pcha_c25447_g2_i1 ‐201 P21 protein (Cdc42/Rac)‐activated kinase 1 ENSGACT00000017971 Pgeo_c58516_g1_i1 Pcha_c43273_g2_i1 sema domain transmembrane domain (TM) and cytoplasmic domain ENSGACT00000017971 Pgeo_c63246_g1_i1 Pcha_c23400_g1_i1 sema6e‐201 (semaphorin) 6E ENSGACT00000018065 Pgeo_c61192_g3_i1 Pcha_c42981_g3_i1 ENSGACT00000018065 Pgeo_c61192_g2_i1 Pcha_c42981_g2_i5 ENSGACT00000018065 Pgeo_c61192_g4_i1 Pcha_c52471_g1_i1 ENSGACT00000018065 Pgeo_c61192_g10_i1 Pcha_c42981_g1_i1 bicd1a‐201 bicaudal D homologue 1a ENSGACT00000018117 Pgeo_c61056_g1_i1 Pcha_c46213_g3_i2 ENSGACT00000018117 Pgeo_c62361_g1_i1 Pcha_c42880_g1_i2 myo18ab‐201 myosin XVIIIAb ENSGACT00000018291 Pgeo_c62120_g9_i1 Pcha_c32872_g3_i1 ENSGACT00000018291 Pgeo_c62120_g7_i1 Pcha_c36501_g1_i1 ENSGACT00000018291 Pgeo_c62120_g10_i1 Pcha_c32872_g1_i1 pde4a‐201 phosphodiesterase 4A cAMP‐specific ENSGACT00000018304 Pgeo_c9911_g1_i1 Pcha_c30662_g1_i2 ENSGACT00000018304 Pgeo_c40511_g1_i1 Pcha_c32800_g1_i2 prkcsh‐201 protein kinase c substrate 80K‐H ENSGACT00000018719 Pgeo_c25228_g1_i1 Pcha_c43281_g1_i1 ENSGACT00000018719 Pgeo_c57973_g1_i1 Pcha_c36318_g1_i1 lfng‐201 LFNG O‐fucosylpeptide 3‐beta‐N‐acetylglucosaminyltransferase ENSGACT00000018752 Pgeo_c18198_g1_i1 Pcha_c37703_g1_i2 ENSGACT00000018752 Pgeo_c48386_g1_i1 Pcha_c20878_g2_i1 ccnd1‐201 cyclin D1 ENSGACT00000018830 Pgeo_c55837_g1_i2 Pcha_c28302_g2_i1 ENSGACT00000018830 Pgeo_c57848_g4_i1 Pcha_c63485_g1_i1 ENSGACT00000018830 Pgeo_c55837_g2_i1 Pcha_c28302_g1_i1 znf536‐201 zinc finger protein 536 ENSGACT00000018922 Pgeo_c61581_g1_i1 Pcha_c45850_g1_i2 ENSGACT00000018922 Pgeo_c33598_g1_i1 Pcha_c42279_g1_i1 rusc2‐201 RUN and SH3 domain containing 2 ENSGACT00000018987 Pgeo_c7369_g1_i1 Pcha_c23478_g1_i1 ENSGACT00000018987 Pgeo_c47406_g1_i1 Pcha_c40727_g2_i2 filip1l‐202 filamin A interacting protein 1‐like ENSGACT00000019028 Pgeo_c44859_g2_i1 Pcha_c44608_g2_i2 ENSGACT00000019028 Pgeo_c44420_g1_i1 Pcha_c35612_g1_i1 si:ch211‐89f7.1‐201 SEC14 2 family member ENSGACT00000019088 Pgeo_c18986_g1_i1 Pcha_c68508_g1_i1 ENSGACT00000019088 Pgeo_c52817_g1_i1 Pcha_c122456_g1_i1 ENSGACT00000019088 Pgeo_c47921_g1_i1 Pcha_c44605_g4_i1 tgm1l1‐201 transglutaminase 1 like 1 ENSGACT00000019099 Pgeo_c34858_g1_i1 Pcha_c49951_g1_i1 ENSGACT00000019099 Pgeo_c10001_g1_i1 Pcha_c35427_g1_i1 kcnj16‐201 potassium inwardly rectifying channel subfamily J member 16 ENSGACT00000019205 Pgeo_c56994_g2_i1 Pcha_c43039_g2_i1 ENSGACT00000019205 Pgeo_c45200_g1_i1 Pcha_c42054_g1_i3 fam83fa‐201 family with sequence similarity 83 member Fa ENSGACT00000019244 Pgeo_c61527_g2_i1 Pcha_c36968_g1_i1 ENSGACT00000019244 Pgeo_c61527_g1_i1 Pcha_c36968_g3_i1 cacng2‐201 calcium channel voltage dependent gamma subunit 2 ENSGACT00000019469 Pgeo_c62042_g3_i1 Pcha_c46199_g11_i2 ENSGACT00000019469 Pgeo_c37370_g1_i1 Pcha_c36138_g1_i3 creb1b‐201 CAMP responsive element binding protein 1b ENSGACT00000019719 Pgeo_c49475_g1_i1 Pcha_c22636_g1_i1 ENSGACT00000019719 Pgeo_c56903_g1_i1 Pcha_c39652_g1_i1 march7‐201 membrane associated ring finger (C3HC4) 7 ENSGACT00000019874 Pgeo_c61406_g1_i1 Pcha_c41808_g1_i3 ENSGACT00000019874 Pgeo_c63021_g1_i1 Pcha_c45633_g2_i1 novel gene rho GTPase activating family member ENSGACT00000020107 Pgeo_c50819_g1_i1 Pcha_c38075_g1_i1 ENSGACT00000020107 Pgeo_c50819_g2_i1 Pcha_c38075_g2_i1 gyg2‐201 glycogenin 2 ENSGACT00000020158 Pgeo_c59420_g5_i1 Pcha_c41098_g2_i3 ENSGACT00000020158 Pgeo_c59477_g3_i1 Pcha_c41371_g1_i4 mbtd1‐201 mbt domain containing 1 ENSGACT00000020191 Pgeo_c58151_g1_i1 Pcha_c40981_g1_i1 ENSGACT00000020191 Pgeo_c62257_g2_i2 Pcha_c43684_g1_i1 arhgap1‐201 rho GTPase activating protein 1 ENSGACT00000020218 Pgeo_c62329_g4_i1 Pcha_c46509_g3_i3 ENSGACT00000020218 Pgeo_c62329_g7_i1 Pcha_c46509_g6_i1 ENSGACT00000020218 Pgeo_c62329_g2_i1 Pcha_c46509_g4_i3 unc13a‐201 unc‐13 homologue ENSGACT00000020305 Pgeo_c63792_g4_i1 Pcha_c40817_g4_i1 ENSGACT00000020305 Pgeo_c59091_g1_i1 Pcha_c42974_g3_i2 ENSGACT00000020305 Pgeo_c63792_g1_i1 Pcha_c47565_g1_i1 jmjd1cb‐201 jumonji domain containing 1Cb ENSGACT00000020326 Pgeo_c70622_g1_i1 Pcha_c10556_g1_i1 ENSGACT00000020326 Pgeo_c59548_g2_i1 Pcha_c45849_g4_i1 ENSGACT00000020326 Pgeo_c59548_g1_i1 Pcha_c45849_g9_i1 epha6‐201 eph receptor A6 ENSGACT00000020449 Pgeo_c62481_g10_i1 Pcha_c34814_g1_i1 ENSGACT00000020449 Pgeo_c62481_g11_i1 Pcha_c95101_g1_i1 clstn2‐201 calsyntenin 2 ENSGACT00000020451 Pgeo_c48064_g2_i1 Pcha_c38049_g1_i1 ENSGACT00000020451 Pgeo_c54810_g1_i1 Pcha_c46038_g1_i1 spsb4b‐201 SplA/ryanodine receptor domain and SOCS box containing 4b ENSGACT00000021080 Pgeo_c59824_g2_i1 Pcha_c38868_g2_i1 ENSGACT00000021080 Pgeo_c59192_g1_i1 Pcha_c39657_g2_i1 clockb‐201 clock circadian regulator b ENSGACT00000021148 Pgeo_c59342_g1_i1 Pcha_c38553_g1_i1 ENSGACT00000021148 Pgeo_c59342_g2_i1 Pcha_c37750_g1_i7 serhl‐201 serine ‐like ENSGACT00000021196 Pgeo_c61220_g1_i1 Pcha_c629_g1_i1 ENSGACT00000021196 Pgeo_c63286_g2_i2 Pcha_c40769_g1_i1 atg4da‐202 autophagy related 4D cysteine peptidase a ENSGACT00000021281 Pgeo_c57535_g2_i1 Pcha_c41931_g3_i1 ENSGACT00000021281 Pgeo_c57535_g1_i1 Pcha_c41931_g2_i1 hk1 (1‐to‐many)‐201 hexokinase 1 ENSGACT00000021336 Pgeo_c14692_g1_i1 Pcha_c5788_g2_i1 ENSGACT00000021336 Pgeo_c37042_g1_i1 Pcha_c35139_g1_i1 hsd17b7‐201 hydroxysteroid (17‐beta) dehydrogenase 7 ENSGACT00000021483 Pgeo_c11948_g1_i1 Pcha_c33976_g1_i2 ENSGACT00000021483 Pgeo_c11948_g2_i1 Pcha_c33976_g2_i1 dhx29‐201 DEAH (Asp‐Glu‐Ala‐His) box polypeptide 29 ENSGACT00000021571 Pgeo_c62250_g1_i1 Pcha_c16841_g1_i2 ENSGACT00000021571 Pgeo_c59361_g2_i1 Pcha_c46068_g9_i1 sh3pxd2aa‐201 SH3 and PX domains 2Aa ENSGACT00000021641 Pgeo_c50942_g1_i1 Pcha_c39656_g1_i1 ENSGACT00000021641 Pgeo_c11735_g1_i1 Pcha_c42857_g1_i1 tmed9‐201 transmembrane p24 trafficking protein 9 ENSGACT00000021694 Pgeo_c24427_g1_i1 Pcha_c37947_g1_i1 ENSGACT00000021694 Pgeo_c7944_g1_i1 Pcha_c41791_g1_i1 yme1l1b‐201 YME1‐like 1b ENSGACT00000021711 Pgeo_c61966_g7_i1 Pcha_c40188_g1_i2 amyloid beta (A4) precursor protein binding family B member 1 interacting ENSGACT00000021711 Pgeo_c62754_g6_i1 Pcha_c43951_g1_i1 apbb1lp (1‐to‐many)‐201 protein ENSGACT00000021793 Pgeo_c141_g1_i1 Pcha_c22679_g1_i1 ENSGACT00000021793 Pgeo_c86448_g1_i1 Pcha_c105855_g1_i1 adh5‐202 alcohol dehydrogenase 5 ENSGACT00000022192 Pgeo_c61051_g2_i1 Pcha_c50268_g1_i1 ENSGACT00000022192 Pgeo_c60505_g3_i1 Pcha_c42347_g6_i3 ENSGACT00000022192 Pgeo_c61051_g1_i1 Pcha_c37433_g1_i1 ntng1a‐201 netrin g1a ENSGACT00000022328 Pgeo_c58670_g1_i1 Pcha_c42081_g4_i1 ENSGACT00000022328 Pgeo_c2232_g1_i1 Pcha_c45456_g1_i4 slc39a1‐201 solute carrier family 39 (zinc transporter)member 1 ENSGACT00000022699 Pgeo_c63099_g7_i2 Pcha_c38655_g3_i3 ENSGACT00000022699 Pgeo_c56599_g1_i1 Pcha_c11925_g2_i1 kcng1‐201 potassium voltage gated channel KQT‐like subfamily member 1 ENSGACT00000022830 Pgeo_c38273_g1_i1 Pcha_c45236_g1_i1 ENSGACT00000022830 Pgeo_c63725_g1_i1 Pcha_c45579_g1_i1 novel gene uncharacterised ENSGACT00000022992 Pgeo_c60595_g2_i1 Pcha_c42718_g1_i2 ENSGACT00000022992 Pgeo_c60595_g3_i1 Pcha_c42718_g2_i1 rc3h2‐201 ring finger and CCCH‐type domains 2 ENSGACT00000023071 Pgeo_c49700_g1_i1 Pcha_c41714_g1_i1 ENSGACT00000023071 Pgeo_c49700_g2_i1 Pcha_c41714_g2_i1 zbtb33‐201 zinc finger and BTB domain containing 33 ENSGACT00000023197 Pgeo_c33755_g1_i1 Pcha_c36414_g2_i1 ENSGACT00000023197 Pgeo_c38086_g1_i1 Pcha_c32479_g1_i3 ENSGACT00000023197 Pgeo_c33755_g2_i1 Pcha_c36414_g1_i3 npnt‐201 nephronectin ENSGACT00000023222 Pgeo_c43927_g2_i1 Pcha_c22022_g1_i1 ENSGACT00000023222 Pgeo_c57493_g1_i1 Pcha_c32440_g2_i1 ENSGACT00000023222 Pgeo_c53957_g6_i1 Pcha_c30885_g1_i1 dip2c (1‐to‐many)‐201 disco interacting protein 2 homologue C ENSGACT00000023729 Pgeo_c61054_g1_i2 Pcha_c26063_g1_i2 ENSGACT00000023729 Pgeo_c22669_g1_i1 Pcha_c32573_g1_i2 si:dkey‐162b23.4‐201 mitochondrial sodium/hydrogen exchanger 9B2 family member ENSGACT00000023762 Pgeo_c61389_g1_i1 Pcha_c45112_g1_i2 ENSGACT00000023762 Pgeo_c61389_g2_i1 Pcha_c43689_g1_i7 agap (1‐to‐many)‐201 ArfGAP with GTPase domain ankyrin repeat and PH domain 1 ENSGACT00000023887 Pgeo_c46613_g2_i1 Pcha_c37439_g2_i1 ENSGACT00000023887 Pgeo_c61706_g1_i1 Pcha_c39468_g1_i2 zbed1 (1‐to‐many)‐201 zinc finger BED‐type containing 1 ENSGACT00000023918 Pgeo_c50720_g1_i1 Pcha_c9858_g1_i1 ENSGACT00000023918 Pgeo_c3357_g2_i1 Pcha_c55259_g1_i1 ENSGACT00000023918 Pgeo_c54321_g1_i1 Pcha_c44330_g1_i3 cacna1bb‐201 calcium channel voltage dependent N type alpha 1B subunit b ENSGACT00000024014 Pgeo_c40490_g1_i1 Pcha_c44664_g1_i1 ENSGACT00000024014 Pgeo_c11312_g1_i1 Pcha_c33029_g1_i2 tsc1b‐201 1b ENSGACT00000024035 Pgeo_c45852_g1_i1 Pcha_c39100_g2_i1 ENSGACT00000024035 Pgeo_c57630_g5_i1 Pcha_c35108_g2_i1 gsna‐203 gelsolin a ENSGACT00000024112 Pgeo_c51955_g4_i1 Pcha_c31949_g2_i1 ENSGACT00000024112 Pgeo_c51955_g3_i1 Pcha_c31949_g3_i1 ENSGACT00000024112 Pgeo_c51955_g2_i1 Pcha_c31949_g1_i1 sec14l1 (1‐to‐many)‐201 SEC14‐like lipid binding 1 ENSGACT00000024212 Pgeo_c46601_g1_i1 Pcha_c42806_g2_i1 ENSGACT00000024212 Pgeo_c48993_g1_i1 Pcha_c42806_g1_i3 novel gene spindlin family member ENSGACT00000024328 Pgeo_c11785_g1_i1 Pcha_c11077_g2_i1 ENSGACT00000024328 Pgeo_c57311_g2_i1 Pcha_c4368_g1_i2 adra2c‐201 adrenoceptor alpha 2C ENSGACT00000024635 Pgeo_c14760_g1_i1 Pcha_c123391_g1_i1 ENSGACT00000024635 Pgeo_c65026_g1_i1 Pcha_c78729_g1_i1 pdgfrl‐201 platelet‐derived growth factor receptor‐like ENSGACT00000024642 Pgeo_c48281_g1_i1 Pcha_c38405_g1_i1 ENSGACT00000024642 Pgeo_c58352_g4_i1 Pcha_c26027_g1_i1 fbxw7‐201 F‐box and WD repeat domain containing 7 ENSGACT00000024706 Pgeo_c61210_g1_i1 Pcha_c42700_g1_i2 ENSGACT00000024706 Pgeo_c59506_g1_i1 Pcha_c43234_g3_i6 micu3a‐201 mitochondrial calcium uptake family member 3a ENSGACT00000024730 Pgeo_c40006_g1_i1 Pcha_c19290_g1_i1 ENSGACT00000024730 Pgeo_c61189_g4_i1 Pcha_c35498_g1_i1 tlr2‐201 toll‐like receptor 2 ENSGACT00000024769 Pgeo_c63211_g14_i1 Pcha_c41749_g2_i1 ENSGACT00000024769 Pgeo_c43015_g1_i1 Pcha_c42918_g3_i1 irs4a‐201 insulin receptor substrate 4a ENSGACT00000025069 Pgeo_c61272_g1_i1 Pcha_c35815_g1_i1 ENSGACT00000025069 Pgeo_c48219_g1_i1 Pcha_c37034_g1_i1 lrrn3‐201 leucine rich repeat neuronal 3 ENSGACT00000025093 Pgeo_c61719_g3_i1 Pcha_c45698_g1_i1 ENSGACT00000025093 Pgeo_c13848_g1_i1 Pcha_c34129_g1_i1 ahcyl2‐201 adenosylhomocysteinase‐like 2 ENSGACT00000025151 Pgeo_c1583_g1_i1 Pcha_c39615_g7_i1 ENSGACT00000025151 Pgeo_c32099_g1_i1 Pcha_c47436_g1_i1 pnp5a‐201 purine nucleoside phosphorylase 5a ENSGACT00000025290 Pgeo_c44871_g1_i1 Pcha_c41329_g1_i1 ENSGACT00000025290 Pgeo_c62884_g1_i1 Pcha_c6395_g1_i1 f13a1b‐202 coagulation factor XIII A1 polypeptide b ENSGACT00000025359 Pgeo_c60866_g2_i1 Pcha_c24598_g1_i1 ENSGACT00000025359 Pgeo_c11322_g1_i1 Pcha_c26558_g1_i1 nfil3‐6‐201 nuclear factor interleukin 3 regulated member 6 ENSGACT00000025388 Pgeo_c59315_g5_i1 Pcha_c43667_g2_i1 solute carrier family 7 (amino acid transporter light chain, L system) member ENSGACT00000025388 Pgeo_c46109_g4_i1 Pcha_c39625_g2_i1 slc7a8a‐204 8a ENSGACT00000025483 Pgeo_c52681_g1_i1 Pcha_c43041_g2_i1 ENSGACT00000025483 Pgeo_c33587_g1_i1 Pcha_c43041_g1_i1 rab40c‐201 RAB40C member RAS oncogene family ENSGACT00000025583 Pgeo_c40482_g1_i1 Pcha_c37853_g1_i1 ENSGACT00000025583 Pgeo_c50989_g2_i1 Pcha_c4463_g1_i1 pnpla3‐201 patatin‐like phospholipase domain containing 3 ENSGACT00000026010 Pgeo_c20181_g1_i1 Pcha_c21517_g1_i1 ENSGACT00000026010 Pgeo_c89994_g1_i1 Pcha_c122938_g1_i1 zgc:194679‐201 beta 1 3 galactosyltransferase 2 ENSGACT00000026219 Pgeo_c56197_g2_i1 Pcha_c23575_g1_i1 ENSGACT00000026219 Pgeo_c56828_g1_i1 Pcha_c30366_g1_i1 fam3c‐201 family with sequence similarity 3 member C, precursor family member ENSGACT00000026341 Pgeo_c63210_g6_i1 Pcha_c12361_g1_i1 ENSGACT00000026341 Pgeo_c55598_g1_i1 Pcha_c63744_g1_i1 fam113‐201 family with sequence similarity 113, PC esterase domain containing ENSGACT00000026462 Pgeo_c59822_g1_i1 Pcha_c34062_g1_i1 ENSGACT00000026462 Pgeo_c65271_g1_i1 Pcha_c22253_g1_i1 capn2l‐202 calpain 2 (m/II) large subunit like ENSGACT00000026505 Pgeo_c55797_g2_i1 Pcha_c36063_g1_i1 ENSGACT00000026505 Pgeo_c45447_g5_i1 Pcha_c122094_g1_i1 epdl1‐201 ependymin‐like 1 ENSGACT00000026518 Pgeo_c32223_g1_i1 Pcha_c41168_g1_i2 ENSGACT00000026518 Pgeo_c88513_g1_i1 Pcha_c20798_g1_i1 mterf2‐201 mitochondrial transcription termination factor 2 ENSGACT00000026776 Pgeo_c62052_g2_i1 Pcha_c46068_g12_i1 ENSGACT00000026776 Pgeo_c62052_g9_i1 Pcha_c46068_g15_i1 atp2a3‐202 ATPase Ca++ transporting ubiquitous ENSGACT00000026854 Pgeo_c58487_g2_i1 Pcha_c46648_g2_i2 ENSGACT00000026854 Pgeo_c25832_g1_i1 Pcha_c31614_g1_i1 slc8a4a‐202 solute carrier family 8 (sodium/calcium exchanger) member 4a ENSGACT00000026915 Pgeo_c45517_g2_i1 Pcha_c11108_g1_i1 ENSGACT00000026915 Pgeo_c53249_g1_i1 Pcha_c42681_g1_i1 cx39.9‐201 connexin 39.9 ENSGACT00000027016 Pgeo_c16761_g1_i2 Pcha_c30105_g1_i1 ENSGACT00000027016 Pgeo_c59191_g1_i1 Pcha_c29540_g2_i1 si:ch211‐284e13.4‐201 insulin receptor substrate 1B family member ENSGACT00000027502 Pgeo_c53074_g1_i1 Pcha_c4482_g1_i1 ENSGACT00000027502 Pgeo_c61282_g1_i1 Pcha_c38923_g1_i2 tmem120a‐201 transmembrane protein 120A ENSGACT00000027632 Pgeo_c41646_g1_i1 Pcha_c31299_g1_i1 ENSGACT00000027632 Pgeo_c61976_g4_i1 Pcha_c43341_g1_i1 rsf1b.1‐201 remodelling and spacing factor 1b tandem duplicate 1

Duplicated, but only present in a single species pgeo only ENSGACT00000000088 Pgeo_c63057_g8_i1 Pcha_c79668_g1_i1 ENSGACT00000000088 Pgeo_c63057_g6_i1 Pcha_c35817_g1_i4 thsd7aa‐201 thrombospondin type 1, domain containing 7Aa ENSGACT00000000314 Pgeo_c59536_g5_i1 Pcha_c46032_g2_i1 ENSGACT00000000314 Pgeo_c37162_g1_i1 Pcha_c46032_g15_i1 ENSGACT00000000314 Pgeo_c59536_g1_i1 Pcha_c46032_g5_i1 myh7bb‐201 myosin heavy chain 7B, cardiac muscle beta b ENSGACT00000008391 Pgeo_c60224_g1_i2 Pcha_c23547_g1_i1 ENSGACT00000008391 Pgeo_c39180_g1_i1 Pcha_c23547_g2_i1 tubb1‐201 tubulin beta 1 class VI ENSGACT00000015111 Pgeo_c61226_g12_i1 Pcha_c45838_g5_i1 ENSGACT00000015111 Pgeo_c54243_g2_i1 Pcha_c38612_g2_i2 ENSGACT00000015111 Pgeo_c54243_g1_i1 Pcha_c38612_g3_i1 prkcea‐201 protein kinase epsilon a ENSGACT00000018978 Pgeo_c61226_g17_i1 Pcha_c45676_g5_i2 ENSGACT00000018978 Pgeo_c61226_g18_i1 Pcha_c45676_g8_i1 prkcb (1‐to‐many)‐201 protein kinase C, beta ENSGACT00000025180 Pgeo_c62203_g2_i1 Pcha_c42402_g4_i2 ENSGACT00000025180 Pgeo_c62203_g15_i1 Pcha_c42402_g1_i1 brd1a‐202 bromodomain containing 1a

pcha only ENSGACT00000003941 Pgeo_c63424_g2_i1 Pcha_c46737_g5_i1 ENSGACT00000003941 Pgeo_c63424_g1_i1 Pcha_c46737_g4_i1 col6a6‐201 collagen type VI alpha 6 ENSGACT00000005516 Pgeo_c60205_g2_i1 Pcha_c43241_g1_i2 ENSGACT00000005516 Pgeo_c43261_g2_i1 Pcha_c33380_g2_i1 lamb2‐201 laminin beta 2 ENSGACT00000008777 Pgeo_c62646_g2_i1 Pcha_c45868_g2_i4 ENSGACT00000008777 Pgeo_c62646_g3_i1 Pcha_c110307_g1_i1 kirrel3a‐201 kin of IRRE like 3a ENSGACT00000017929 Pgeo_c31414_g1_i1 Pcha_c5006_g1_i1 ENSGACT00000017929 Pgeo_c58588_g1_i1 Pcha_c44386_g7_i2 hipk2‐201 homeodomain interacting protein kinase 2 ENSGACT00000019206 Pgeo_c37643_g2_i1 Pcha_c35555_g1_i2 ENSGACT00000019206 Pgeo_c37643_g1_i1 Pcha_c45497_g1_i1 march6 (1‐to‐many)‐201 membrane associated ring finger (C3HC4)6

Supplementary Table S3: Duplicated genes (more than two copies) in the Antarctic fish. Identification of the number of orthologues in other Ensembl listed fish species and Homo sapiens compared with the stickleback. Key: 0 = absent; 1 = present 1:1 (1 copy in stickleback and 1 copy in the designated species); M = 1: many (1 copy in stickleback and many copies in the designated species); MM = many: many (multiple orthologues are found in both species).

Gene ID Stickleback ID Oreochromis Takifugu Tetraodon Oryzias Xiphophorus Poecilia Gadus Danio Astyanax Lepisosteus Homo niloticus rubripes nigroviridis latipes maculatus formosa morhua rerio mexicanus oculatus sapiens 5x Tln2a ENSGACT00000021683 0 1 1 1 1 1 1 1 1 M M

4x Lrp1bb ENSGACT00000010024 1 1 1 1 M 0 1 1 1 1 1 Prkg1a ENSGACT00000012119 1 1 1 1 1 1 1 1 1 M M Pcdh2g12 ENSGACT00000023710 MM MM 0 1 0 MM 1 MM MM 1 MM

3x si:ch73‐ ENSGACT00000004996 1 1 1 1 1 1 1 1 1 M M Prkd3 ENSGACT00000009267 1 1 1 1 1 1 1 1 1 1 1 Smurf2 ENSGACT00000009315 1 1 1 1 1 1 M 0 0 M M Mast3b ENSGACT00000011412 1 1 1 1 1 1 1 1 1 M M St3gal1 ENSGACT00000011682 M 0 0 0 MM M MM MM MM M M cel ENSGACT00000024004 1 0 1 M M M 1 MM M M M Supplementary Table S4: Duplicated genes (two copies or more) in the Antarctic fish in the Panther enriched pathway for CCKR signalling. Identification of the number of orthologues in other Ensembl listed fish species and Homo sapiens compared with the stickleback. Key: 0 = absent; 1 = present 1:1 (1 copy in stickleback and 1 copy in the designated species); M = 1: many (1 copy in stickleback and many copies in the designated species).

Gene ID Stickleback ID Oreochromis Takifugu Tetraodon Oryzias Xiphophorus Poecilia Gadus Danio Astyanax Lepisosteus Homo niloticus rubripes nigroviridis latipes maculatus formosa morhua rerio mexicanus oculatus sapiens Mapk14a ENSGACT00000010242 1 1 M 1 1 1 1 M 1 1 M Arhgef28 ENSGACT00000005803 1 1 1 1 1 1 1 0 0 1 1 Pak1 ENSGACT00000017559 1 1 1 1 1 1 1 1 1 1 1 Ptena ENSGACT00000012260 1 1 1 1 1 1 1 1 1 M M Ccnd1 ENSGACT00000018752 1 1 1 1 1 1 1 1 1 1 1 Prkcea ENSGACT00000015111 1 1 1 1 1 1 1 1 1 1 1 Prkg1a ENSGACT00000012119 1 1 1 1 1 1 1 1 1 M M Map2k6 ENSGACT00000014497 1 1 1 1 1 1 1 1 0 1 1