ultrafiltration

Ultrafiltration Products Contents

Major uses for ultrafiltration 2 Process alternatives 3 Membrane characteristics 4 Membrane selection 5 Vivaspin 500 6 Vivaspin 2 8 Centrisart I 11 Vivaspin 4 14 Vivaspin 6 16 Vivaspin 15 18 Vivaspin 15R 20 Vivaspin 20 22 Vivacell 70 26 Vivacell 100 29 Vivacell 250 32 Vivaflow 50 34 Vivaflow 200 37 Vivapore 2, 5, 10/20 40 Purification Products 42 Vivapure Ion Exchange 43 Vivapure Metal Chelate 45 Vivapure Protein A Kit 47 Vivapure Epoxy Kit 48 Vivapure Anti-HSA/IgG Kit 49 Vivapure C18 50 Vivapure AdenoPack 100 51 Flitration Products 52 Minisart 53 Sartolab 56 Biolab instruments 57

1 Major uses for ultrafiltration

Ultrafiltration is a convective Typical applications for • Purification of antibiotics, process that uses anisotropic ultrafiltration hormones, drugs from biological semi-permeable membranes to • Concentration/desalting of fluids, fermentation broths separate macromolecular species proteins, enzymes, DNA, • Recovery of biomolecules from cell and solvents primarily on the monoclonal antibodies, culture supernatants, lysates basis of size. It is particularly immunoglobulins • General purpose laboratory appropriate for the concentration • Free drug, hormone assays concentration and desalting of of macromolecules and can also • Removal of primers from PCR proteins, enzymes, cells, DNA, be used to purify molecular amplified DNA biomolecules, antibodies and species or for solvent exchange. • Removal of labelled amino acids immunoglobulins Ultrafiltration is a gentle, non and nucleotides • Mammalian cell harvesting denaturing method that is more • HPLC sample preparation • Cell washing, virus purification, cell efficient and flexible than • Deproteinization of samples debris removal, depyrogenation alternative processes.

Solute concentration Ultrafiltration membranes are used to increase the solute concentration of a desired biological species. The filtrate is cleared of macromolecules which are significantly larger than the retentive membrane pores. Microsolute is removed convectively with the solvent.

Solute fractionation or clarification Ultrafiltration is a cost effective method for separating samples into size-graded components providing that macromolecular fractions differ in size by a 10x MW difference. During filtration, the permeating solute remains at its initial concentration whilst the retained macromolecules will be enriched.

Solute desalting or purification A solution may be purified from salts, non-aqueous solvents and generally from low molecular weight materials. Multiple solvent exchanges, will progressively purify macromolecules from contaminating solutes. Microsolutes are removed most efficiently by adding solvent to the solution being ultrafiltered at a rate equal to the speed of filtration. This is called diafiltration.

2 Process alternatives

Vivascience offers a comprehensive range of process alternatives for the filtration and concentration of biological samples. Below is a guide to selecting the most suitable filtration method, depending on sample volume, equipment available, filtration speed and process control desired.

Centrifugal filtration (100 µl to 100 ml starting volumes) characterised by quick set up and fast Centrifugation provides the vector to filtration speeds with most solutions. clear solvent and micro molecules Vivascience offers ten alternative through the ultrafiltration membrane centrifugal devices covering volumes and into a filtrate container positioned from 100 µl up to 100 ml. below. This is a gentle process that is

Pressure-fugation (5 to 50 ml starting volumes) than centrifugation alone. Pressure-fugation is a unique Vivaspin 20 and Vivacell 70 can Vivascience method that combines gas be run in this way. pressure with centrifugation. This is the fastest concentration method with process times typically 30 to 50% faster

Gas pressure filtration (5 to 250 ml starting volume) For fastest filtration, Vivacell products Pressurised air or an inert gas is used are used with an orbital laboratory to provide the filtration vector. Agitation shaker but they can also be used is used to impede macromolecules without agitation. Vivaspin 20, Vivacell from polarising on the membrane 70, Vivacell 100 and Vivacell 250 can be surface and reducing filtration speed. run with gas pressure.

Tangential flow filtration (100 ml to several litres starting volume) as solvent and micro-molecules pass The solution to be processed is pumped through the membrane into a separate under pressure across an ultrafiltration filtrate vessel. Vivaflow 50 and 200 are membrane and then returned to the offered for this procedure. original reservoir. The solution is progressively concentrated or purified

Solvent absorption (1 to 20 ml starting volume) are concentrated into the bottom of the This technique uses an absorbent sample container. No additional cellulose pad mounted behind the equipment is required. Five Vivapore ultrafiltration membrane to draw devices are offered for this procedure solvents and micro solutes through the with maximum initial sample volumes membrane. Retained macromolecules ranging from 1 to 20 ml.

3 Membrane performance characteristics

Vivascience offers an extended Polyethersulfone Regenerated cellulose range of membranes to cover the This is a general purpose membrane These Membranes are also highly great majority of ultrafiltration that provides excellent performance hydrophillic and are often preferred for requirements. with most solutions when retentate their higher protein recovery when The following is a guide to recovery is of primary importance. processing some very dilute solutions. selecting the most appropriate Polyethersulfone membranes exhibit Resistance to autoclaving, ease of membranes according to their no hydrophobic or hydrophillic cleaning and extended chemical typical performance interactions and are usually preferred resistance also characterises this type characteristics. Please note for their low fouling characteristics, of membrane. however, that membrane exceptional flux and broad pH range. behaviour and ultimate performance, largely depends on Cellulose triacetate Hydrosart® the specific characteristics of the High hydrophilicity and very low non Hydrosart demonstrates the same solution being processed. specific binding characterise this properties as regenerated cellulose, Vivascience recommends that membrane. Cast without any but with the added benefit of users experiment with membrane support that could trap or enhanced performance characteristics alternative membranes in order bind passing micro solutes, these and extremely low protein binding, to optimise their process membranes are preferred for sample making it the membrane of choice for performance. cleaning and protein removal and applications such as concentration when high recovery of the filtrate and desalting of immunoglobulin solution is of primary importance. fractions.

Membrane performance comparisons

Relative solute flux* Membrane Frequently preferred for: (ml/min/cm2) Polyethersulfone pH range 1-14 3,000 MWCO 0.05 Very high retention of peptides 5,000 MWCO 0.24 High retention of peptides, high relative flux 10,000 MWCO 0.41 Versatility, high flux, low adsorption 30,000 MWCO 0.41 Versatility, high flux 50,000 MWCO 0.45 Sharp molecular weight limit 100,000 MWCO 0.35 High retention of Immunoglobulins Cellulose triacetate pH range 4-8 5,000 MWCO 0.04 Peptide and protein removal 10,000 MWCO 0.11 Micro-partition, free/bound drug studies 20,000 MWCO 0.58 Sample cleaning, HPLC sample preparation Regenerated cellulose pH range 3-11 10,000 MWCO 0.18 High recovery of microgram quantities of protein 30,000 MWCO 0.58 Speed and recovery with immunoglobulins 100,000 MWCO 0.40 Protein fractionation Hydrosart® pH range 1-14 5,000 MWCO 0.14 High recovery of very dilute solutions 10,000 MWCO 0.27 High flux, high recovery, low adsorption 30,000 MWCO 0.48 High recovery of immunoglobulins *0.25mg/ml BSA or IgG depending on MWCO at 4 bar pressure.

4 Membrane selection guide

Membrane selection guide (recommended MWCO)

Application < 5,000 10,000 30,000 50,000 100,000 > 300,000 Bacteria DNA fragments Enzymes Growth factors Immunoglobulins Nucleic Acids MAB Oligonucleotides Peptides Virus Yeast For highest recovery, select a membrane MWCO which is at least half of the molecular weight of the solute to be retained

The advanced designs and low Typically a higher cut-off membrane • Select the lowest MWCO membrane adsorption materials that will bind more than a low molecular that suits the application. characterise Vivascience weight alternative. products, offer a unique • When available, prefer swing bucket combination of faster processing Adsorption to the sample to fixed angle rotors. This reduces speeds and higher recovery of container the surface area of the concentrator the concentrated sample. Although every effort is made to that will be exposed to the solution Providing that the appropriate minimise this phenomenon by the during centrifugation. device size and membrane cut-off selection of low adsorption materials is selected, Vivascience products and tool production to optical • Reduce pressure or centrifugal force will typically yield recoveries of standards, some solute will bind to to approximately half of the the concentrated sample in the internal surface of the sample maximum recommended. excess of 90% when the starting container. Whilst the relative sample contains over 0.1 mg/ml adsorption will be proportionately less • Avoid over concentration. The of the solute of interest. important than on the membrane, due smaller the final concentrate Most of the loss is caused by non to the higher total surface area, this volume, the more difficult it is to specific binding both to the can be the major source of yield loss. achieve complete recovery. If membrane surface and to feasible, after a first recovery, rinse exposed binding sites on the Process optimisation the device with one or more drops plastic of the sample container: When highest recoveries are most of buffer and then recover again. important, in particular when working Adsorption to the membrane with solute quantities in the • Pretreat the device overnight with a Depending on sample characteristics microgram range, Vivascience passivation solution such as 5% SDS, relative to the membrane type used, recommends that users consider the Tween 20, or Triton X in distilled solute adsorption on the membrane following: water. Then rinse thoroughly surface is typically 2-10 µg/cm2. This before use. can increase to 20-100 µg/cm2 when • Select the smallest device that suits the filtrate is of interest and the the sample volume. Additionally, take solute must pass through the whole advantage of the extra speed of internal structure of the membrane. Vivascience products by refilling a smaller device repeatedly. 5 Vivaspin 500

100 µl to 600 µl samples Vivaspin 500 µl centrifugal filter units offer a simple, one step procedure for sample preparation. They can effectively be used in either swing out or fixed angle rotors accepting 2.2 ml centrifuge tubes.

The patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations, even with particle laden solutions.

Technical specifications Vivaspin 500 Concentrator capacity Swing bucket rotor 600 µl Fixed angle rotor 600 µl Dimensions Total length 50 mm Width 11 mm 50 Active membrane area 0.5 cm2 Hold-up volume, membrane and support < 5 µl Dead stop volume 5 µl Materials of construction Body Polycarbonate Filtrate vessel Polypropylene Concentrator cap Polycarbonate Membrane Polyethersulfone 11

6 Vivaspin 500

Equipment required Vivaspin 500 Centrifuge Rotor type Fixed angle Minimum rotor angle 40˚ Rotor cavity To fit 2.2 ml (11 mm) conical bottom tubes Maximum speed 15,000 g Concentrate recovery Pipette type Fixed or variable volume Recommended tip Thin gel loader type

Typical performance Time to concentrate 30x min. at 20˚C and solute recovery % Rotor Fixed angle Centrifugal force 12,000 g Start volume 500 µl Min. Rec. Aprotinin 0.25mg/ml (6,500 MW) 3,000 MWCO PES 30 96 % BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 15 96 % 10,000 MWCO PES 5 96 % 30,000 MWCO PES 5 95 % IgG 0.25 mg/ml (160,000 MW) 30,000 MWCO PES 10 96 % 50,000 MWCO PES 10 96 % 100,000 MWCO PES 10 96 %

Ordering information Vivaspin 500 Polyethersulfone Pack size Prod. no. 3,000 MWCO 25 VS0191 3,000 MWCO 100 VS0192 5,000 MWCO 25 VS0111 5,000 MWCO 100 VS0112 10,000 MWCO 25 VS0101 10,000 MWCO 100 VS0102 30,000 MWCO 25 VS0121 30,000 MWCO 100 VS0122 50,000 MWCO 25 VS0131 50,000 MWCO 100 VS0132 100,000 MWCO 25 VS0141 100,000 MWCO 100 VS0142 300,000 MWCO 25 VS0151 300,000 MWCO 100 VS0152 1,000,000 MWCO 25 VS0161 1,000,000 MWCO 100 VS0162 0.2 µm 25 VS0171 0.2 µm 100 VS0172 Starter pack (5 of each 5 k, 10 k, 30 k, 50 k, 100 k) 25 VS01S1

7 Vivaspin 2 New membranes

0.4 - 2 ml samples The Vivaspin 2 bridges the gap between the 500 µl and 4 ml centrifugal concentrators. This device combines the speed of the classic Vivaspin products with low internal surface and membrane area for superior recoveries from very dilute solutions.

Available with a choice of PES, Cellulose Triacetate, Regenerated Cellulose and Hydrosart® membranes, Vivaspin 2 offers the highest flexibility for process optimisation.

Also unique to the Vivaspin 2, is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concentrator, or alternatively reverse spinning into the concentrate recovery cap which can then be sealed for storage. Both methods Technical specifications Vivaspin 2 result in near total concentrate Concentrator capacity recoveries. Swing bucket rotor 3 ml Fixed angle rotor 2 ml 17 Dimensions Total length 126 mm Width 17 mm

2 10,000 Active membrane area 1.2 cm MWCO Hold-up volume of membrane <10 µl 2.0 Dead stop volume 8 µl 1.5

1.0 Materials of construction

126 0.5 Body Polycarbonate Filtrate vessel Polycarbonate 0.2

0.1 Concentrator cap Polycarbonate

20µl Membrane PES, CTA, RC, HY

8 Vivaspin 2

Equipment required Vivaspin 2 Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle - 25° Rotor cavity To fit 15 ml (17 mm) To fit 15 ml (17 mm) conical bottom tubes conical bottom tubes Maximum speed 4,000 g 12,000 g* Concentrate recovery Pipette type Fixed or variable volume Fixed or variable volume Recommended tip Thin gel loader type Thin gel loader type * Please note, devices with membrane MWCO >100,000 kDa need to be processed at lower g forces. See data sheets for details.

Typical performance Time to concentrate 30x min. at 20˚C and solute recovery % Rotor Fixed angle Centrifugal force 5,000 g Start volume 2 ml Min. Rec. Aprotinin 0.25mg/ml (6,500 MW) 3,000 MWCO PES 50 96 % BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 12 98 % 5,000 MWCO CTA 50 96 % 5,000 MWCO Hydrosart 22 98 % 10,000 MWCO PES 8 98 % 10,000 MWCO RC 14 98 % 10,000 MWCO CTA 10 96 % 10,000 MWCO Hydrosart 12 98 % 20,000 MWCO CTA 5 96 % 30,000 MWCO PES 8 97 % 30,000 MWCO RC 5 98 % 30,000 MWCO Hydrosart 5 97 % IgG 0.25 mg/ml (160,000 MW) 20,000 MWCO CTA 6 97 % 30,000 MWCO PES 10 96 % 30,000 MWCO RC 9 97 % 50,000 MWCO PES 10 96 % 100,000 MWCO PES 8 95 % 100,000 MWCO RC 4 96 %

9 Vivaspin 2

Crystal clear polycarbonate construction

PES, CTA, RC, or Hydrosart® membranes Direct pipette recovery or choice Integral deadstop avoids of reverse spinning risk of concentrating concentrate into to dryness sample cap

Filtrate and Filtrate container concentrate can be fits standard 15ml sealed for storage tube carriers

Ordering information Vivaspin 2 Polyethersulfone Pack size Prod. no. Vivaspin 2 Cellulose triacetate Pack size Prod. no. 3,000 MWCO 25 VS0291 5,000 MWCO 25 VS02U1 3,000 MWCO 100 VS0292 5,000 MWCO 100 VS02U2 5,000 MWCO 25 VS0211 10,000 MWCO 25 VS02V1 5,000 MWCO 100 VS0212 10,000 MWCO 100 VS02V2 10,000 MWCO 25 VS0201 20,000 MWCO 25 VS02X1 10,000 MWCO 100 VS0202 20,000 MWCO 100 VS02X2 30,000 MWCO 25 VS0221 Vivaspin 2 Regenerated cellulose 30,000 MWCO 100 VS0222 10,000 MWCO 25 VS02K1 50,000 MWCO 25 VS0231 10,000 MWCO 100 VS02K2 50,000 MWCO 100 VS0232 30,000 MWCO 25 VS02L1 100,000 MWCO 25 VS0241 30,000 MWCO 100 VS02L2 100,000 MWCO 100 VS0242 100,000 MWCO 25 VS02M1 300,000 MWCO 25 VS0251 100,000 MWCO 100 VS02M2 300,000 MWCO 100 VS0252 Vivaspin 2 Hydrosart 1,000,000 MWCO 25 VS0261 2,000 MWCO 25 VS02H91 1,000,000 MWCO 100 VS0262 2,000 MWCO 100 VS02H92 0.2 µm 25 VS0271 5,000 MWCO 25 VS02H11 0.2 µm 100 VS0272 5,000 MWCO 100 VS02H12 Starter pack 25 VS02S1 10,000 MWCO 25 VS02H01 (5 of each 5 k, 10 k, 30 k, 50 k, 100 k) 10,000 MWCO 100 VS02H02 30,000 MWCO 25 VS02H21 30,000 MWCO 100 VS02H22

Ordering Tips • Choose a membrane pore size at least 50% smaller than the size of the molecule to be retained. • Usually choose Polyethersulfone membranes for fastest concentrations. • Usually choose Cellulose Triacetate for Protein Removal/Ultrafiltrate recovery. • Usually choose Hydrosart® membranes for highest recovery with Ig fractions.

10 Centrisart I

0.5 - 2.5 ml samples Centrisart I is a ready to use unit for small volume centrifugal ultrafiltration to separate proteins from low molecular weight substances in biological samples. Centrisart I features a unique design, ultrafiltration in the opposite direction to the centrifugal force. This is so effective in preventing premature blockage of the filter that even whole blood samples can be deproteinized. The ultrafiltrate is collected in the floater insert tube, where it is readily accessible without removing the tube.

Typical applications include: • drug binding studies • determination of metabolites in serum • protein removal from blood samples • cleaning of liposomes • virus removal

Technical specifications Centrisart I Direction of Concentrator capacity ultrafiltration Swing bucket rotor 2.5 ml Fixed angle rotor 2.5 ml Dimensions Total length 93 mm Ultrafiltrate is Width 14 mm collected in the floater Active membrane area 0.79 cm2 insert tube, where it is Hold-up volume of membrane < 5 µl readily accessible without removal of Dead stop volume 100 µl the tube. Materials of construction Centrifuge tube Polystyrene Floater tube Cellulose propionate Cap Polyethylene Ultrafiltrate Membrane CTA, PES Ultrafilter Retentate

Centrifugal force

11 Centrisart I

Equipment required Vivaspin 2 Easy-to-use Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle - 25° Rotor cavity To fit 15 ml (17 mm) To fit 15 ml (17 mm) conical bottom tubes conical bottom tubes Maximum speed 2,500 g 2,000 g Concentrate recovery Pipette type Fixed or variable volume Fixed or variable volume Recommended tip Thin gel loader type Thin gel loader type Remove interior tube, pour in sample

Time to filter Time to filter Passage of Typical performance 50% of sample 90% of sample sample species volume volume BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO 300 min N/A 0% 10,000 MWCO 35 min 80 min 2% Replace interior tube 20,000 MWCO 9 min 20 min 2% IgG 0.25 mg/ml (160,000 MW) 100,000 MWCO 13 min 35 min 3% Blue Dextran 0.1 mg/ml (2,000,000 MW) 300,000 MWCO 9 min 25 min 28% *2.5 ml samples were loaded into each device. The devices were centrifuged at 2,000 g until the required filtrate volumes had been reached.

Centrifuge

Pipette out …or use forceps to the filtrate… remove the interior tube to access the concentrate

12 Centrisart I

Ordering information Pack size Prod. no. References 5,000 MWCO CTA 12 13229-E P. Nebinger and M. Koel 10,000 MWCO CTA 12 13239-E Determination of acyclovir by 20,000 MWCO CTA 12 13249-E ultrafiltration and high-performance 100,000 MWCO PES 12 13269-E liquid chromatography. 300,000 MWCO PES 12 13279-E J. Chromatography 619, 342-344 (1993) Starter pack (3 units each 12 13209-E of 5k, 10k, 20k, 100k) F. da Fonseca-Wollheim, K.-G. Heinze, K. Lomsky and H. Schreiner Serum ultrafiltration for the elimination of endogenous interfering substances in creatinine determination. J.Clin.Chem.Clin.Biochem. 26, 523-525 (1988)

R. H. Christenson, S. D. Studenberg, S. Beck-Davis and F. A. Sedor Digoxin-like immunoreactivity eliminated from serum by centrifugal ultrafiltration before fluorescence polarization immunoassay of digoxin. Clinical Chemistry 33, 606-608 (1987)

13 Vivaspin 4

1 - 4 ml samples Vivaspin 4 ml concentrators are disposable ultrafiltration devices for the concentration of biological samples. Maximum initial sample volumes range from 1 ml to 4 ml. They can be effectively used in either swing bucket or fixed angle rotors accepting 15 ml centrifuge tubes.

The patented vertical membrane design and thin channel filtration chamber (US 5,647,990) minimises membrane fouling and provides high speed concentrations, even with particle laden solutions.

Vivaspin 4 is available with the high flux polyethersulfone membrane range which is recommended for most solutions.

17 Technical specifications Vivaspin 4 Concentrator capacity Swing bucket rotor 4 ml Fixed angle rotor 4 ml Dimensions Total length 122 mm Width 17 mm Active membrane area 2.0 cm2 122 Hold-up volume of membrane <10 µl Dead stop volume 20 µl Materials of construction Body Polycarbonate Filtrate vessel Polypropylene Concentrator cap Polycarbonate Membrane Polyethersulfone

14 Vivaspin 4

Equipment required Vivaspin 4 Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle - 25° Rotor cavity To fit 15 ml (17 mm) To fit 15 ml (17 mm) conical bottom tubes conical bottom tubes Maximum speed 4,000 g 10,000 g* Concentrate recovery Pipette type Fixed or variable volume Fixed or variable volume Recommended tip Thin gel loader type Thin gel loader type * Please note, devices with membrane MWCO >100,000 kDa need to be processed at lower g forces. See data sheets for details.

Typical performance Time to concentrate 30x min. at 20˚C and solute recovery % Rotor Fixed angle Centrifugal force 5,000 g Start volume 4 ml Min. Rec. BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 15 96% 10,000 MWCO PES 10 96% 30,000 MWCO PES 10 95% IgG 0.25 mg/ml (160,000 MW) 30,000 MWCO PES 10 95% 50,000 MWCO PES 10 95% 100,000 MWCO PES 10 95%

Ordering information Vivaspin 4 Polyethersulfone Pack size Prod. no. 5,000 MWCO 25 VS0413 5,000 MWCO 100 VS0414 10,000 MWCO 25 VS0403 10,000 MWCO 100 VS0404 30,000 MWCO 25 VS0423 30,000 MWCO 100 VS0424 50,000 MWCO 25 VS0433 50,000 MWCO 100 VS0434 100,000 MWCO 25 VS0443 100,000 MWCO 100 VS0444 0.2 µm 25 VS0473 0.2 µm 100 VS0474 Starter pack (5 of each 5 k, 10 k, 30 k, 50 k, 100 k) 25 VS04S3

15 Vivaspin 6

2 - 6 ml samples Vivaspin 6 ml concentrators have been developed to offer increased volume flexibility and performance.

Vivaspin 6 can process an impressive 6 ml in either swing bucket or fixed angle rotors accepting standard 15 ml conical bottom test tubes.

The Vivaspin 6 features twin vertical membranes for unparalleled filtration speeds and 100x plus concentrations. Remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipette recovery of the final concentrate.

Technical specifications Vivaspin 6 6 Concentrator capacity Swing bucket rotor 6 ml 4 Fixed angle rotor 6 ml Dimensions 2 Total length 122 mm Width 17 mm 122 Active membrane area 2.5 cm2 0.5

0.3 Hold-up volume of membrane <10 µl 0.2 0.1 Dead stop volume 30 µl Materials of construction Body Polycarbonate Filtrate vessel Polycarbonate Concentrator cap Polypropylene Membrane Polyethersulfone

17

16 Vivaspin 6

Equipment required Vivaspin 6 Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle - 25° Rotor cavity To fit 15 ml (17 mm) To fit 15 ml (17 mm) conical bottom tubes conical bottom tubes Maximum speed 4,000 g 10,000 g* Concentrate recovery Pipette type Fixed or variable volume Fixed or variable volume Recommended tip Thin gel loader type Thin gel loader type * Please note, devices with membrane MWCO >100,000 kDa need to be processed at lower g forces. See data sheets for details.

Typical performance Time to concentrate 30x min. at 20˚C and solute recovery % Rotor Swing bucket 25˚ Fixed angle Centrifugal force 3,000 g 7,500 g Start volume 6 ml 6 ml Min. Rec. Min. Rec. Cytochrome c 0.25 mg/ml (12,400 MW) 5,000 MWCO PES - - 90 97 % BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 20 98 % 12 98 % 10,000 MWCO PES 13 98 % 10 98 % 30,000 MWCO PES 12 98 % 9 97 % IgG 0.25 mg/ml (160,000 MW) 30,000 MWCO PES 18 96 % 15 95 % 50,000 MWCO PES 17 96 % 14 95 % 100,000 MWCO PES 15 91 % 12 91 % Latex beads 0.004 % in DMEM +10 % FCS (0.055 µm) 300,000 MWCO PES - - 25 99 % Latex beads 0.004 % in DMEM +10 % FCS (0.24 µm) 1,000,000 MWCO PES - - 4 99 % Yeast 1.0 mg/ml (S. Cerevisiae) 0.2 µm PES 4 97 % 3 97 %

Ordering information Vivaspin 6 Polyethersulfone Pack size Prod. no. Pack size Prod. no. 5,000 MWCO 25 VS0611 100,000 MWCO 100 VS0642 5,000 MWCO 100 VS0612 300,000 MWCO 25 VS0651 10,000 MWCO 25 VS0601 300,000 MWCO 100 VS0652 10,000 MWCO 100 VS0602 1,000,000 MWCO 25 VS0661 30,000 MWCO 25 VS0621 1,000,000 MWCO 100 VS0662 30,000 MWCO 100 VS0622 0.2 µm 25 VS0671 50,000 MWCO 25 VS0631 0.2 µm 100 VS0672 50,000 MWCO 100 VS0632 Starter pack 25 VS06S1 100,000 MWCO 25 VS0641 (5 of each 5 k, 10 k, 30 k, 50 k, 100 k)

17 Vivaspin 15

2 - 15 ml samples The Vivaspin 15 concentrator is a disposable ultrafiltration device for use in swing bucket centrifuges accommodating 50 ml tubes. Vivaspin 15 is used for the concentration of biological samples in the 2 - 15 ml range. The innovative design (US Patent no. 5,647,990, second patent pending), simplicity, speed and exceptional concentrate recoveries are the main features of the concentrator.

In a single spin, 15 ml solutions can be concentrated up to 300x. Samples can be typically concentrated in 10-30 minutes with macromolecular recoveries in excess of 95%. The longitudinal membrane location and adjacent thin channel, provide optimum cross flow conditions even for particle laden solutions, the centrifugal force pulling particles and solids away from the membrane to the bottom of the device. Macromolecules collect in an impermeable 50 µl concentrate pocket integrally moulded below the

membrane surface, thereby Technical specifications Vivaspin 15 eliminating the risk of filtration Concentrator capacity to dryness. Swing bucket rotor 15 ml Fixed angle rotor 8 ml 31 Dimensions Total length 76 mm Width 25.5 mm Active membrane area 4 cm2 Hold up volume of membrane <20 µl 76 Dead stop volume 50 µl 30,000 MWCO 1ml Materials of construction

0.75

0.50 Body Polycarbonate

025 0.10 Filtrate vessel Polypropylene Concentrator cap Polycarbonate Membrane Polyethersulfone 25.5

18 Vivaspin 15

Equipment required Vivaspin 15 Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle - 25° Rotor cavity To fit 15 ml (17 mm) To fit 15 ml (17 mm) conical bottom tubes conical bottom tubes Maximum speed 3,000 g* 3,000 g Concentrate recovery Pipette type Fixed or variable volume Fixed or variable volume Recommended tip Thin gel loader type Thin gel loader type * Please note, devices with membrane MWCO >100,000 kDa need to be processed at lower g forces. See data sheets for details.

Typical performance Time to concentrate 30x min. at 20˚C and solute recovery % Rotor Fixed angle Centrifugal force 2,000 g Start volume 15 ml Min. Rec. BSA 1 mg/ml (66,000 MW) 5,000 MWCO 40 97% 10,000 MWCO 25 97% 30,000 MWCO 25 96% 50,000 MWCO 25 96% 100,000 MWCO 15 70% Cytochrome c 0.25 mg/ml (12,400 MW) 5,000 MWCO 55 97% 10,000 MWCO 45 95% 30,000 MWCO 45 59% 50,000 MWCO 45 40% 100,000 MWCO 20 16% IgG 0.25 mg/ml (160,000 MW) 30,000 MWCO 30 94% 50,000 MWCO 30 94% 100,000 MWCO 30 90% Yeast 1.0 mg/ml (S. Cerevisiae) 100,000 MWCO 15 98% 0.2 µm PES 7 95%

Ordering information - Requires 50 ml centrifuge tubes Vivaspin 15 Polyethersulfone Pack size Prod.no. Pack size Prod.no. 5,000 MWCO 10 VS1511 100,000 MWCO 10 VS1541 5,000 MWCO 40 VS1512 100,000 MWCO 40 VS1542 10,000 MWCO 10 VS1501 0.2 µm 10 VS1571 10,000 MWCO 40 VS1502 0.2 µm 40 VS1572 30,000 MWCO 10 VS1521 Starter pack 10 VS15S1 30,000 MWCO 40 VS1522 (2 of each 5 k, 10 k, 30 k, 50 k, 100 k) 50,000 MWCO 10 VS1531 Accessories 50,000 MWCO 40 VS1532 Conical bottom 50 ml tubes and lids 100VSA001 19 Vivaspin 15R New

2 - 15 ml samples Vivaspin 15R is the latest member of the Vivaspin product family with all the unique features of Vivascience concentrators including a patented vertical membrane and a dead stop. Vivaspin 15R is targeting the volume segment 2 to 15 ml with a modified regenerated cellulose membrane; Hydrosart®. This membrane is ideal where extremely high recovery with very low adsorption is needed, for example in applications such as desalting and concentration of Ig fractions.

• Ultimate recovery at low Adsorption (95-98%) • Extremely short concentration time (30x in 15 min.) • Convenient application protocol with easy handling • Easy scale-up to Vivaflow 200 with Hydrosart® membrane for volumes up to 5 litres • Very small hold up volume (< 20 µl)

Technical specifications Vivaspin 15R Concentrator capacity 15 Swing bucket rotor 15 ml 10 Fixed angle rotor 12.5 ml 5 Dimensions Total length 116 mm

116 Width 30 mm Active membrane area 3.9 cm2 Hold up volume membrane < 20 µl Dead stop volume 30 µl Materials of construction Body Polycarbonate Filtrate vessel Polypropylene Concentrator cap Polycarbonate Membrane Hydrosart 30

20 Vivaspin 15R

Equipment required Vivaspin 15R Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle - 25° Rotor cavity To fit 50 ml (30 mm) To fit 50 ml (30 mm) conical bottom tubes conical bottom tubes Maximum speed 3,000 g 6,000 g Concentrate recovery Pipette type Fixed or variable volume Fixed or variable volume Recommended tip Thin gel loader type Thin gel loader type

Typical performance Time to concentrate 30x min. at 20˚C Spin and solute recovery % Rotor Swing bucket 25˚ Fixed angle Centrifugal force 3,000 g 6,000 g Start volume 15 ml 12.5 ml Min. Rec. Min. Rec. Aprotinin 0.1 mg/ml* (6,500 MW) 5,000 MWCO 47 95 % 45 95 % Cytochrome c 0.25 mg/ml* (12,400 MW) 5,000 MWCO 45 96 % 45 96 % 10,000 MWCO 25 94 % 18 94 % α-chymotrypsin 0.25 mg/ml* (25,000 MW) 5,000 MWCO 50 98 % 45 98 % 10,000 MWCO 25 98 % 18 98 % Ovalbumin 1.0 mg/ml* (45,000 MW) 10,000 MWCO 20 98 % 14 98 % 30,000 MWCO 15 94 % 12 94 % BSA 1.0 mg/ml* (66,000 MW) Recover 30,000 MWCO 18 98 % 15 98 % IgG 0.1 mg/ml*in DMEM (160,000 MW) 30,000 MWCO 30 98 % 25 96 % * proteins other than IgG made up in 50 mM potassium phosphate, 150 mM sodium chloride, pH 7.4

Ordering information Vivaspin 15R Hydrosart Pack size Prod. no. 2,000 MWCO 12 VS15RH91 2,000 MWCO 48 VS15RH92 5,000 MWCO 12 VS15RH11 5,000 MWCO 48 VS15RH12 10,000 MWCO 12 VS15RH01 10,000 MWCO 48 VS15RH02 30,000 MWCO 12 VS15RH21 30,000 MWCO 48 VS15RH22

21 Vivaspin 20

5 - 20 ml samples Vivaspin 20 ml centrifugal concentrators have been developed to offer increased volume flexibility and performance.

Vivaspin 20 handles up to 20 ml in swing bucket centrifuges and 14 ml in 25˚ fixed angle rotors accepting 50 ml centrifuge tubes.

Featuring twin vertical membranes for unparalleled filtration speeds the Vivaspin 20 can achieve 100x plus concentrations.

Remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipette recovery of the final concentrate.

Technical specifications Vivaspin 20

20 Concentrator capacity Swing bucket rotor 20 ml 15 Fixed angle rotor 14 ml 10 With pressure head 15 ml 5 Dimensions Total length 116 mm 1 0.75 125 mm with pressure head 116 0.5 0.2 Width 30 mm Active membrane area 6.0 cm2 Hold up volume of membrane < 20 µl Dead stop volume 50 µl Materials of construction Body Polycarbonate Filtrate vessel Polycarbonate Concentrator cap Polypropylene Pressure head Acetal/aluminium Membrane Polyethersulfone 30

22 Vivaspin 20

More process flexibility Vivaspin 20 is available with unique accessories and operating methods that are designed to provide more process flexibility and further time saving.

Gas pressure filtration When an appropriate centrifuge is unavailable, or for single sample processing, Vivaspin 20 can be filled with up to 15 ml and then pressurised for bench top concentration. For even faster processing, gas pressure can be combined with centrifugal force. "pressure-fugation" is particularly suitable for difficult or viscous samples such as serum, or when using a low process temperature which reduces filtration speed, and generally when minimum process time is essential.

Using the Vivaspin 20 pressure cap

EITHER

PRESSURISE SPIN up to 5 bar with up to 3,000 x g air or inert gas

THEN OR RECOVER

STAND IN RACK

23 Vivaspin 20

One step desalting In this procedure following concentration, a diafiltration cup is Desalting of Concentrated Sample filled with buffer and then spun one time to achieve 98% salt removal. This compares to the need for two Diafiltration cup is filled with buffer solution spins to achieve the same result with the traditional refill and re-spin procedure. The improved performance is due to Solvent in sample is continuously replaced the constant washing action of the by fresh buffer solution buffer solution in the diafiltration cup as it replaces solvent and salts as they pass through the ultrafiltration membrane.

Remaining salts and contaminants are progressively cleared through membrane

Equipment required Vivaspin 20 Centrifuge Rotor type Swing bucket Fixed angle Minimum rotor angle - 25° Rotor cavity To fit 50 ml (30 mm) To fit 50 ml (30 mm) conical bottom tubes conical bottom tubes Maximum speed 5,000 g* 8,000 g* Optional pressure accessories Air pressure controller (APC) complete with pressure gauge, regulator, over-pressure safety valve, female connector to Vivascience pressure Prod no. VCA002 products and 1m extension line (4 mm pneumatic tubing) with male and female connectors and 1 m of 6 mm inlet tubing Charge valve Prod no. VCA005 VS20 pressure head Prod. no.VCA200 Concentrate recovery Pipette type Fixed or variable volume Fixed or variable volume Recommended tip Thin gel loader type Thin gel loader type * Please note, devices with membrane MWCO >100,000 kDa need to be processed at lower g forces. See data sheets for details.

24 Vivaspin 20

Typical performance Time to concentrate 30x min. at 20˚C and solute recovery % Mode Centrifuge Centrifuge Bench top Press-fuge Rotor Swing bucket 25˚ Fixed angle Pressure Swing bucket Centrifugal speed/pressure 3,000 g 6,000 g 4 bar 3,000 g + 4 bar Start volume 20 ml 14 ml 10 ml 10 ml Min. Rec. Min. Rec. Min. Rec. Min. Rec. Cytochrome c 0.25 mg/ml (12,400 MW) 3,000 MWCO PES 110 97 % 180 96 % 60 96 % - - BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 23 99 % 29 99 % 50 98 % 14 98 % 10,000 MWCO PES 16 98 % 17 98 % 32 97 % 8 97 % 30,000 MWCO PES 13 98 % 15 98 % 32 97 % 8 97 % IgG 0.25 mg/ml (160,000 MW) 30,000 MWCO PES 27 97 % 20 95 % 46 94 % 13 97 % 50,000 MWCO PES 27 96 % 22 95 % 46 93 % 13 96 % 100,000 MWCO PES 25 91 % 20 90 % 42 88 % 12 94 % Latex beads 0.004 % in DMEM +10 % FCS (0.055 µm) 300,000 MWCO PES 20 99 % 35 99 % 10 99 % - - Latex beads 0.004 % in DMEM +10 % FCS (0.24 µm) 1,000,000 MWCO PES 4 99 % 12 99 % 4 99 % - - Yeast 1.0 mg/ml (S. Cerevisiae) 0.2 µm PES 15 95 % 5 95 % 20 95 % 2 95 %

Ordering information Vivaspin 20 Polyethersulfone Pack size Prod. no. 3,000 MWCO 12 VS2091 3,000 MWCO 48 VS2092 5,000 MWCO 12 VS2011 5,000 MWCO 48 VS2012 10,000 MWCO 12 VS2001 10,000 MWCO 48 VS2002 30,000 MWCO 12 VS2021 30,000 MWCO 48 VS2022 50,000 MWCO 12 VS2031 50,000 MWCO 48 VS2032 100,000 MWCO 12 VS2041 100,000 MWCO 48 VS2042 300,000 MWCO 12 VS2051 300,000 MWCO 48 VS2052 1,000,000 MWCO 12 VS2061 1,000,000 MWCO 48 VS2062 0.2 µm 12 VS2071 0.2 µm 48 VS2072 Starter pack 12 VS20S1 (2 of each 5 k, 10 k, 30 k, 50 k, 100 k, 0.2 µm) Vivaspin 20 accessories Air pressure controller (APC) 1 VCA002 Charge valve for pressure head 1 VCA005 Diafiltration cups 12 VSA005 Female connector 1 VCA010 Male connector 1 VCA011 4 mm OD pneumatic tube (3 m) 1 VCA012 Vivaspin 20 pressure head 1 VCA200 25 Vivacell 70

10 - 70 ml samples Vivacell 70 combines the ease of use of centrifugal devices with the flexibility and control provided by pressurised ultrafiltration cells. Vivacell 70 is inexpensive, quick and easy to assemble, requires no tubing connections or stirring mechanisms and can be adapted to equipment availability or to specific user preferences.

For convenience, simply spin in a large capacity centrifuge (rotors accepting 250 ml bottles). For highest speeds particularly with difficult samples, pressurise the device with air or inert gas before centrifuging.

For more process control or for single samples, combine gas pressure with a gentle orbital shake, or you can even pressurise and then leave standing on a bench top or in a refrigerator for highest simplicity with minimum equipment requirements.

The longitudinal membrane inhibits fouling, whilst the built-in dead stop will hinder further concentration when residual volume drops below 150 µl.

Centrifuge Pressurise Pressure-shake Pressure-fuge • Process convenience • Simplicity and highest • Speed and process control • Fastest processing • Low shear, no foaming process control • Ideal for single samples • Ideal with low MWCO or • Less visual control • Ideal for refrigerated use • If left unattended can with difficult solutions • Slower concentrations concentrate to dryness • Less visual control

26 Vivacell 70

Technical specifications Vivacell 70 Concentrator capacity Swing bucket rotor 70 ml Fixed angle rotor 50 ml With pressure head 70 ml With pressure-fuge head 50 ml Dimensions 119 mm standard centrifugal centrifugal mode centrifugal mode Total length 185 mm with pressure head swing-out rotors fixed-angle rotors 125 mm with pressure fuge head modified cap Width 62 mm Active membrane area 20 cm2 Hold up volume of membrane <200 µl Dead stop volume 150 µl Operating requirements Rotor type Swing bucket or fixed angle Minimum rotor angle 25 ˚ Rotor cavity To fit 250 ml (62 mm) centrifuge bottles pressure mode pressure mode bench top centrifuge Maximum speed 1,000 g Maximum pressure 5 bar (75 psi) Materials of construction Body Polycarbonate Filtrate vessel Polycarbonate Total Concentrator cap Santoprene = process Pressure head/pressure fuge head Acetal flexibility Membrane Polyethersulfone

storage mode standard cap

Typical performance Time to concentrate 30x min at 20˚C 50ml Start volume In centrifuge 1,000 g As pressure cell 4 bar (60 psi) pressure Solute No pressure 3 bar pressure No agitation Orbital shake recovery % BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 37 18 50 25 96 % 10,000 MWCO PES 25 15 45 20 96 % 30,000 MWCO PES 22 13 45 20 93 % IgG 0.25 mg/ml (160,000 MW) 50,000 MWCO PES 25 15 85 20 94 % 100,000 MWCO PES 15 11 90 18 90 %

27 Vivacell 70

Ordering information Vivacell 70 Polyethersulfone - concentrator bodies with polycarbonate filtrate bottles Pack size Prod. no. 5,000 MWCO 2 VS6011 10,000 MWCO 2 VS6001 30,000 MWCO 2 VS6021 50,000 MWCO 2 VS6031 100,000 MWCO 2 VS6041 0.2 µm 2 VS6071 Vivacell 70 Polyethersulfone - concentrator body only 5,000 MWCO 10 VS6012 10,000 MWCO 10 VS6002 30,000 MWCO 10 VS6022 50,000 MWCO 10 VS6032 100,000 MWCO 10 VS6042 0.2 µm 10 VS6072 Vivacell 70 accessories Air pressure controller (APC) complete with pressure gauge, regulator, over-pressure safety valve, female connector to Vivascience pressure 1 VCA002 products and 1 m extension line (4 mm pneumatic tubing) with male and female connectors and 1 m of 6 mm inlet tubing 250 ml centrifuge bottle - standard caps 4 VSA003 Modified caps for use in fixed angle rotors with 250 ml centrifuge bottles 2 VCA004 Charge valve for pressure-fuge head 1 VCA005 Replacement seals for pressure-fuge head (VCA701) 10 VCA007 Female connector 1 VCA010 Male connector 1 VCA011 4 mm pneumatic tubing (3 m) 1 VCA012 Vivacell 70 pressure head with reservoir and filtrate bottle (bench top use) 1 VCA700 Vivacell 70 pressure-fuge head (for use in centrifuge) 2 VCA701

28 Vivacell 100 New

20 - 100 ml Samples Vivacell 100 is the latest member of the Vivacell family and bridges the volume range between the Vivacell 70 and the Vivacell 250.

The patented vertical membrane design allows highest performance and unmatched flexibility.

Vivacell 100 is a unique and innovative concentrator for volumes from 20 ml to 100 ml, which utilizes pressure, centrifuge or pressure-shake to rapidly concentrate even samples with very high particle loading.

Vivacell 100 is designed for centrifugal concentration of samples up to 100 ml which makes it the largest centrifugal unit available. At the same time, the new construction design allows for maximum centrifugal force of 4,000x g to be used for even faster concentration.

Technical specifications Vivacell 100 Concentrator capacity Swing bucket rotor 90 ml With pressure head 98 ml Dimensions Total length 123 mm centrifugal 197 mm with pressure head Width 62 mm Active membrane area 23.5 cm2 Hold up volume of membrane <250 µl Dead stop volume 350 µl Operating requirements Rotor type Swing bucket Rotor cavity To fit 250 ml (62 mm) centrifuge bottles (maximum cavity depth 105 mm) Maximum speed 2,000 g Maximum pressure 5 bar (75 psi) Materials of construction Body Polycarbonate Vivacell 100 utilizes: Filtrate vessel Polycarbonate • Pressure Concentrator cap Santoprene • Centrifuge Pressure head Acetal • Pressure-shake Membrane Polyethersulfone

29 Vivacell 100

Like the smaller Vivacell 70 unit, Vivacell 100, when used as a Quick release connector centrifugal device, fits into swing bucket rotors accepting 250 ml bottles.

Vivacell 100 units can also be used for single or extremely sensitive samples in the pressurized mode only and left on the bench or placed on a Pressure head Santoprene seal laboratory shaker for faster concentration. It can also be kept in a Concentrator cap pressurized mode in the refrigerator. Handling is made easy by use of quick Graduations connectors. In whichever mode Vivacell 100 is used, the vertical Concentrator membrane design inhibits membrane Twin vertical membranes fouling while the built-in dead stop impedes concentration to dryness and loss of sample.

Filtrate container fits standard 250 ml rotors

Centrifuge Pressure Pressure-shake • Process convenience • Simplicity and • Speed and process control • Low shear, no foaming highest process control • Ideal for single samples • Less visual control • Ideal for refrigerated use • Slower concentrations

30 Vivacell 100

Typical performance Time to concentrate 30x min. at 20˚ C 90 ml start volume In centrifuge 2,000 g As pressure cell 4 bar (60 psi) pressure Solute swing out rotor No agitation Orbital shake recovery % BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 22 75 25 96 % 10,000 MWCO PES 16 60 20 96 % 30,000 MWCO PES 16 60 20 94 % IgG 0.25 mg/ml (160,000 MW) 50,000 MWCO PES 20 70 30 94 % 100,000 MWCO PES 20 85 30 90 % Latex beads 0.004 % in DMEM + 10 % FCS (0.055 µm) 300,000 MWCO PES 35 - 120 99 % Latex beads 0.004 % in DMEM + 10 % FCS (0.24 µm) 1,000,000 MWCO† PES 4 5 4 99 % †2,000 g in centrifuge, 2 bar (29 psi) pressure

Ordering information Vivacell 100 Polyethersulfone With Polypropylene concentrator cap Pack size Prod. no. Accessories Pack size Prod. no. 5,000 MWCO 2 VC1011 Air pressure controller (APC) 5,000 MWCO 10 VC1012 complete with pressure gauge, 10,000 MWCO 2 VC1001 regulator, over-pressure safety valve, 10,000 MWCO 10 VC1002 female connector, 1 m extension line 1 VCA002 30,000 MWCO 2 VC1021 (4 mm pressure tubing) with male 30,000 MWCO 10 VC1022 and female connectors and 1 m of 50,000 MWCO 2 VC1031 6 mm inlet tubing 50,000 MWCO 10 VC1032 Plastic pipettes 100 VPA005 100,000 MWCO 2 VC1041 Female connector 1 VCA010 100,000 MWCO 10 VC1042 Male connector 1 VCA011 300,000 MWCO 2 VC1051 4 mm pressure tubing (3 m) 1 VCA012 300,000 MWCO 10 VC1052 Santoprene replacement seals 10 VCA014 1,000,000 MWCO 2 VC1061 Vivacell 100 pressure head with 1 VCA800 1,000,000 MWCO 10 VC1062 replacement seals (5) 0.2 µm 2 VC1071 0.2 µm 10 VC1072

31 Vivacell 250 New features

50 - 250 ml samples The Vivacell 250 is a totally new concept for the concentration of larger biological samples. This product offers numerous advantages when compared to stirred cells.

• One size handles a volume range from under 50 ml to 250 ml. • Use free standing on a bench top or in a refrigerator for maximum simplicity, or use on laboratory shaker for fastest concentrations. • The unique conical dead stop built into the bottom of the membrane insert allows concentrations to under 1 ml. • The gentle vortex action controls membrane polarisation whilst greatly reducing the shear effects typical of stirring mechanisms. • Set up or membrane replacement takes just a few seconds. Quick connect fittings and simple screw closure further enhance ease of use.

Technical specifications Vivacell 250 Concentrator capacity 250 ml Max pressure 4 bar (60 psi) Dimensions Width 116 mm Height (incl. pressure indicator) 235 mm Active membrane area 40 cm2 Hold-up vol. memb. & support <200 µl Dead stop volume 600 µl Materials of construction Screw closure Acetal Pressure head Acetal Unique membrane module takes seconds to Quick release connector Acetal replace. Concentrate can be easily monitored Concentrator body/sleeve Polycarbonate through the graduated inspection window. Filtrate container Polycarbonate

32 Vivacell 250

Typical performance Time to concentrate 20x min. at 20˚C 4 bar pressure 100 ml start volume 250 ml start volume Orbital Free Solute Orbital Free Solute shake standing recovery % shake standing recovery % BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 19 70 98 % 40 140 99 % 10,000 MWCO PES 12 45 97 % 28 100 98 % 30,000 MWCO PES 12 45 96 % 28 100 98 % γ Globulins 0.25 mg/ml (160,000 MW) 30,000 MWCO PES 25 120 96 % 55 240 98 % 50,000 MWCO PES 25 120 94 % 55 240 98 % 100,000 MWCO PES 25 120 96 % 58 240 98 %

Ordering information Vivacell 250 Pack size Prod. no. Screw Closure Vivacell 250 complete with pressure head, pressure indicator/over-pressure release valve, quick release connection 1 VCA250 Quick connect check valve to APC, 2 sample reservoirs, filtrate container & insert tool Vivacell 250 Polyethersulfone inserts Combined pressure 5,000 MWCO 5 VC2511 indicator and safety valve 10,000 MWCO 5 VC2501

30,000 MWCO 5 VC2521 Membrane module 50,000 MWCO 5 VC2531 takes seconds to 100,000 MWCO 5 VC2541 replace 0.2 µm 5 VC2571 Unique conical base Starter kit (1 of each 5 k, 10 k, 30 k, 50 k, 100 k) 5 VC25S1 allows concentrations to under 1ml Accessories

Air pressure controller (APC) complete with pressure gauge, Concentrate regulator, over-pressure safety valve, female connector to 1 VCA002 inspection window Vivascience pressure products and 1 m extension line (4 mm pneumatic tubing) with male and female connector and 1 m of 6 mm inlet tubing Replacement pressure indicator/over pressure relief valve 1 VCA008 Vivacell 250 maintenance kit (includes one sample reservoir 1 VCA009 and filtrate container, and “O” ring seals for pressure head) Female connector 1 VCA010 Male connector 1 VCA011 4 mm OD pressure tubing (3 m) 1 VCA012 Replacement pressure head & screw closure 1 VCA015

33 Vivaflow 50 New design

100 ml to 5 litres and more The novel Vivaflow 50 system (patents pending) provides a standard of ease of use, performance, flexibility and economy which is unrivalled by any laboratory or pilot scale filtration system on the market.

Unique features • Thin channel flip-flow recirculation path provides high cross flow velocities with minimum pump requirements. • No need for pressure holders. • Crystal clear for simple control of remaining hold up and membrane status. • Unique Interlocking modules with series connectors for easy scale up. • Disposable.

Unique performance • A single 50 cm2 module will typically reduce 500 ml to less than 15 ml in under 50 minutes. • Less than 10 ml minimum system recirculation for highest concentrations. • Less than 500 µl non recoverable hold up volume. • Near total recoveries achievable with a single 10 ml rinse.

Unique “flip-flow” thin channel flow path results in high turbulence and linear velocity for exceptional flux even at high concentrations

34 Vivaflow 50

Technical specifications Vivaflow 50 Dimensions Materials of construction Overall L/H/W 107/84/25 mm Main housing Polycarbonate Channel W/H 15 mm/0.3 mm Flow channel TPX (PMP) Active membrane area 50 cm2 Membrane support TPX (PMP) Hold up volume (module) 1.5 ml Seals and O rings Silicone Minimum recirculation volume <10 ml Pressure indicator Polypropylene, SS spring, Non recoverable hold-up <0.5 ml Flow restrictor Polypropylene Operating conditions Fittings Nylon Pump flow 200-400 ml/min Tubing PVC (medical grade) Maximum pressure 3 bar (45 psi) Maximum temperature 60˚ C

Typical Performance Time to concentrate 20x min. at 3 bar inlet pressure, 20˚ C Single device Three devices Solute recovery % 250 ml start volume 1 L start volume Direct 10 ml rinse BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 34 49 96 % > 99 % 10,000 MWCO PES 22 32 94 % > 99 % 10,000 MWCO RC 38 55 96 % > 99 % 30,000 MWCO PES 22 32 92 % 99 % 30,000 MWCO RC 13 21 96 % 99 % 50,000 MWCO PES 20 29 92 % 98 % γ Globulins 1.0 mg/ml (160,000 MW) 100,000 MWCO PES 43 62 92 % 98 % 100,000 MWCO RC 40 58 92 % 98 % Yeast 1.0 mg/ml (S.Cerevisiae) 0.2 µm PES 33 47 92 % 98 %

Multiple modules Single module

Filtrate vessel

Sample/diafiltration reservoir (VFA006)

35 Vivaflow 50

Ordering information Vivaflow 50 modules include filtrate tube, size 16 peristaltic tubing, flow restrictor and fittings Pack size Prod. no. 3,000 MWCO PES 2 VF05P9 5,000 MWCO PES 2 VF05P1 10,000 MWCO PES 2 VF05P0 30,000 MWCO PES 2 VF05P2 50,000 MWCO PES 2 VF05P3 100,000 MWCO PES 2 VF05P4 0.2 µm PES 2 VF05P7 10,000 MWCO RC 2 VF05C0 30,000 MWCO RC 2 VF05C2 100,000 MWCO RC 2 VF05C4 Vivaflow 50 complete system comprises: Pump (240 V), Easy load pump head (size 16), tubing, 500 ml sample/diafiltration reservoir, module stand, 1 VFS502 pressure indicator, T connectors, series interconnectors Pump (115 V), Easy load pump head (size 16), tubing, 500 ml sample/diafiltration reservoir, module stand, 1 VFS504 pressure indicator, T connectors, series interconnectors Vivaflow 50 PVC tubing and fittings Size 16 PVC pump tubing (3 metres, 3.2 x 1.6 mm) VFA004 Flow restrictor set (2 x 0.4, 0.6, 0.8 mm) VFA009 T connectors for running 2 stacks (2 pieces) VFA030 Series interconnectors (6 pieces) VFA031 Female luer fittings (10 pieces) VFA032 VF50 tubing Kit (2 x 1 m size 16 PVC tubing with inlet fittings, VFA034 2 x 50 cm size 16 PVC tubing with 0.6 mm flow restrictors, 1 x series interconnector) Flow restrictor 0.6 mm (6 pieces) VFA035 VivaFlow 50 accessories Masterflex economy drive variable speed peristaltic pump (240V) VFP001 Masterflex economy drive variable speed peristaltic pump (115V) VFP002 500 ml sample and/or diafiltration reservoir VFA006 Masterflex standard pump head - size 16 VFA010 Masterflex easy load pump head - size 16 VFA012 Vivaflow 50 stand VFA016 Pressure indicator (1-3 bar) VFA020

36 Vivaflow 200 New membranes

0.5 to 5 litres and more Concentrate 250 ml to under 20 ml in just a few minutes or concentrate one litre 50 times in less than 30 minutes. Alternatively, use two Vivaflow 200’s in parallel and concentrate 5 litres in under 75 minutes.

Near total sample recoveries can be expected with most solutions.

The economical standard package comes complete with tubing, pressure indicator, flow restrictor and high pressure pump tubing. All you need is a peristaltic pump capable of handling 6.4 mm OD (size 16) tubing. Should your pump head require larger tubing, link your own peristaltic tube up to the standard product, using the interconnector provided.

Technical specifications Vivaflow 200 Dimensions Overall L/H/W 126/138/38 mm Channel W/H 10 mm/0.4 mm Active membrane area 200 cm2 Hold up volume (module) 5.3 ml Min. recirculation volume < 20 ml Non recoverable hold-up < 2 ml Materials of construction Main housing Acrylic Flow channel Acrylic Membrane support Polypropylene Seals and O rings Silicone Pressure indicator Polypropylene, SS spring Flow restrictor Polypropylene Fittings Nylon Tubing PVC (medical grade) Operating conditions Two modules in parallel will concentrate Pump flow 200-400 ml/min 5 litres in under 75 minutes Maximum pressure 4 bar (60 psi) Maximum temperature 60 ˚C

37 Vivaflow 200

Typical performance Time to concentrate 20x min. at 3 bar inlet pressure, 20˚ C 1 litre Average flux Recovery % start volume ml/min direct 25 ml rinse BSA 1.0 mg/ml (66,000 MW) 5,000 MWCO PES 29 33 98 % > 99 % 5,000 MWCO Hydrosart 70 14 98 % > 99 % 10,000 MWCO PES 23 41 96 % > 99 % 10,000 MWCO RC 42 23 97 % > 99 % 10,000 MWCO Hydrosart 35 27 98 % > 99 % 30,000 MWCO PES 25 38 96 % 99 % 30,000 MWCO RC 22 43 96 % 99 % 30,000 MWCO Hydrosart 20 48 96 % > 99 % 50,000 MWCO PES 22 43 96 % 98 % γ Globulins 1.0 mg/ml (average 160,000 MW) 100,000 MWCO PES 54 18 96 % 99 % 100,000 MWCO RC 45 21 96 % 99 % Yeast 1.0 mg/ml (S. Cerevisiae) 0.2 µm PES 11 86 92 % 98 % Dilute solute concentration, start volume 1 litre at 3 bar, 10,000 MWCO PES BSA 0.001 mg/ml 18 52 90 % 98 % BSA 0.01 mg/ml 20 47 92 % 98 % BSA 0.1 mg/ml 21 45 94 % 99 % Start volume 5 litres (two VF200 in parallel at 3 bar) 10,000 MWCO PES BSA 1.0 mg/ml (66,000 MW) 67 70 97 % > 99 %

Vivaflow 200 set-up for diafiltration Pressure indicator Feed line

Waste

Flow restrictor Feed reservoir

Sealed diafiltration reservoir Pump head

38 Vivaflow 200

Ordering information Vivaflow 200 modules include pressure indicator, flow restrictor and size 16 pvc peristaltic tubing and fittings Pack size Prod. no. 5,000 MWCO PES 1 VF20P1 10,000 MWCO PES 1 VF20P0 30,000 MWCO PES 1 VF20P2 50,000 MWCO PES 1 VF20P3 100,000 MWCO PES 1 VF20P4 0.2 µm PES 1 VF20P7 10,000 MWCO RC 1 VF20C0 30,000 MWCO RC 1 VF20C2 100,000 MWCO RC 1 VF20C4 5,000 MWCO Hydrosart 1 VF20H1 10,000 MWCO Hydrosart 1 VF20H0 30,000 MWCO Hydrosart 1 VF20H2 Vivaflow 200 complete system comprises: Pump (240 V), Easy load pump head (size 16), tubing, 500 ml sample/diafiltration reservoir 1 VFS202 Pump (115 V), Easy load pump head (size 16), tubing, 500 ml sample/diafiltration reservoir 1 VFS204 Vivaflow 200 accessories Masterflex economy drive variable speed peristaltic pump (240V) VFP001 Masterflex economy drive variable speed peristaltic pump (115V) VFP002 500 ml sample and/or diafiltration reservoir VFA006 Masterflex standard pump head - size 16 VFA010 Masterflex standard pump head - size 15 VFA011 Masterflex easy load pump head - size 16 VFA012 Masterflex easy load pump head - size 15 VFA013 Vivaflow 200 tubing and fittings Size 15 pvc pump tubing and Luer fittings (3 m, 4.8 x 2.6 mm)) VFA003 Size 16 pvc pump tubing and Luer fittings (3 m, 3.2 x 1.6 mm)) VFA004 Y connector (size 15 to 2 x size 16) VFA005 Flow restrictor set (2 x 0.4, 0.6, 0.8 mm) VFA009 Female luer fittings size 16 (10 pieces) VFA032 Flow restrictors 0.6 mm (6 pieces) VFA035 Female luer fittings size 15 (10 pieces) VFA036

Operation - Single Module Operation - Two Modules

Return Return

Feed Feed

Sample Sample

Pump Pump Filtrate Filtrate 39 Vivapore Solvent Absorption Concentrators

0.5 ml - 20 ml samples With no need for additional equipment, pressure or vacuum, solvent absorption is the most economic and user friendly concentration technique available to the clinician and research scientist.

Just fill the unit with the solution to be concentrated, wait for the desired concentration level to be achieved and then pipette the concentrated sample from the bottom of the reservoir.

Vivapore is ideal for general purpose laboratory concentration or purification prior to further analysis. It is particularly suited for labile solutions that can denature with alternative shear or pressure inducing methods or that require processing in a cold room environment.

Vivapore concentrators extend the solvent absorption technique to a totally new level of performance, application potential and ease of use.

Technical specifications Vivapore 2 Vivapore 5 Vivapore 10/20 Membrane material Modified PES or regenerated cellulose Membrane MWCO 7,500 PES, 30,000 RC Membrane surface area 15 cm2 20 cm2 28 cm2 Reservoir material TPX, (PMP) SAN SAN Volume range 0.5-2.5 ml/15 ml* 1- 5 ml 2 -10 ml / 20 ml* Minimum concentrate volume 20 µl 50 µl 50 µl Vivapore overall dimensions Width (mm) 66 42 46 Height (mm) 68 82 100

* with additional reservoir

Vivapore 2 Vivapore 5 Vivapore 10/20

40 Vivapore Solvent Absorption Concentrators

Typical performance Time to concentrate (10x min.) Concentrate recovery % Product VP2 VP5 VP10/20 VP10/20* VP2 VP5 VP10/20 VP10/20* Start volume 2 ml 5 ml 10 ml 20 ml 2 ml 5 ml 10 ml 20 ml Cytochrome c (12,600 MW) 0.25 mg/ml 0.25 mg/ml 0.25 mg/ml 0.1 mg/ml 0.25 mg/ml 0.25 mg/ml 0.25 mg/ml 0.1 mg/ml 7,500 MWCO PES 35 35 75 150 90 % 90 % 90 % 92 % 30,000 MWCO RC 30 25 50 105 18 % 18 % 18 % 20 % BSA (66,000 MW) 7,500 MWCO PES 25 30 55 115 90 % 92 % 92 % 92 % 30,000 MWCO RC 20 25 40 80 90 % 90 % 90 % 94 % IgG (160,000 MW) 7,500 MWCO PES 35 40 70 160 76 % 75 % 77 % 78 % 30,000 MWCO RC 25 35 35 80 80 % 82 % 85 % 90 % Time to concentrate 50x (min.) Concentrate recovery % Cytochrome c (12,600 MW) 7,500 MWCO PES 65 70 160 - 91 % 88 % 90 % - 30,000 MWCO RC 55 60 95 - 16 % 16 % 16 % - BSA (66,000 MW) 7,500 MWCO PES 45 50 105 218 90 % 90 % 92 % 94 % 30,000 MWCO RC 40 45 60 120 89 % 88 % 88 % 90 % IgG (160,000 MW) 7,500 MWCO PES 50 65 140 290 53 % 65 % 74 % 70 % 30,000 MWCO RC 45 60 65 135 60 % 70 % 82 % 88 %

* with additional reservoir

Ordering information Vivapore 2 Pack size Prod. no. Vivapore 10/20 Pack size Prod. no. Expandable to 15 ml with pipette reservoir Includes stand and recovery pipettes 7,500 MWCO PES 30 VP0201 7,500 MWCO PES 4 VP2003 30,000 MWCO RC 30 VP0271 7,500 MWCO PES 30 VP2001 Vivapore 5 30,000 MWCO RC 4 VP2073 Includes stand and recovery pipettes 30,000 MWCO RC 30 VP2071 7,500 MWCO PES 4 VP0503 Requires stand 7,500 MWCO PES 30 VP0501 7,500 MWCO PES 100 VP2002 30,000 MWCO RC 4 VP0573 30,000 MWCO RC 100 VP2072 30,000 MWCO RC 30 VP0571 Vivapore accessories Requires stand Disposable stands for 4 units 6 VPA002 7,500 MWCO PES 100 VP0502 Pipette reservoir (Vivapore 2) 50 VPA004 30,000 MWCO RC 100 VP0572 Plastic recovery pipettes (Vivapore 10/20) 100 VPA005 10 ml expansion reservoir (Vivapore 10/20) 10 VPA006 Plastic recovery pipettes (Vivapore 5) 100 VPA007 10 position acrylic stand 1 VPA010

41 Vivapure - the next step in protein purification

Original sample

Protein is bound to membrane

Protein purification in less than 30 minutes 1-2-3 protocol for purification of basic proteins (high pI) with Vivapure Maxi H cation exchanger (S)

Hardware requirements: Ide ally,a be nch cen The separation of pure proteins from can s trifuge th pin a at h t speed ouses 5 or s of 500 0 ml b ientation x g.Ple ucket sw of the V ase note ing-out r Align ivapure in that yo otor an the prin sert a u must c d ted cha nd the lo onside Buffers racter (S wer ma r the ) on the x.volum B insert t e if you uffer A - owards use a fix S the cen ed-ang Bu tre of th le rotor complex mixtures is a key process in ffer B - S 250 mM e rotor. . sodiu m acet 1.5 M N ate buff aCl in 2 er pH 5. S 5 Step 5 mM so (10x co dium a ncentr Sam Proc cetate b ate) ple clea edure uffer pH ring L 5.5 Sample d oad 19 m MA ilution l samp XI Wash Dilute le on cla sample rificatio E of 1:10 in n spin c biomedical research and other quilibrat buffer c Buffer A olumn.5 ion L onc -S (a o entrate fter 1:1 Spee ad 5 ml with dis 0 dilutio d T Buffer A tilled wa n 0 ime Sample of buff -S (after ter). 0 x g loadin er conce 1:10 dilu 5 m g Loa ntrate w tion in d up to 1 ith distille 9 ml s d wat sample is ample (E er). close t nsure th 1st W Reloa o that o at the p ash ste d devic f the loa H of the p W e if sam ding bu prepare 500 biological disciplines. Vivascience is ash spin ple volu ffer). d x g colu me ex 5 min Elu of b mn with ceeds 1 tion uffer co 10 ml B 9 ml. ncentra uffer A Elute s te with -S (after ample 2 distilled 1:10 dilu pre -3 times water).5tion pared b with 10 y dilutin ml of in as indic g Buffer creasin ated on B-S wit g NaCl c Han the dilu h Buffer oncent 00 x g dbook f tion tab A-S and rations, 5 or detail le (see 1 distilled 500 min offering Vivapure spin columns based s). -2-3 Pro water x g to col, P ag 5 min e 20 in Buffer dilution

B uffer A 10 B S x conc. uffer B (m H2 on an innovative and powerful l) (ml) O dist. 0 (m Eluti l) on buff 0.1 er 4 20 conc. 0.2 18 (M 7 .6 0 NaCl 0 ) .34 17.3 1.26 1.50 0.4 16.6 2.43 1.40 0 .54 16 3.06 1.30 0.67 14 3. A - S .6 60 1.25 (1 membrane adsorber technology for 0x con B 0.8 1 4 c.) - S 3.3 .86 1.20 0 .94 12 6.03 1.10 1.0 10.6 7.20 1.00 1 .07 10 8.46 0.90 1.2 9.3 9.10 0.80 1 .34 8 9.63 0.75 For res 1.47 6.6 10.80 0 earch us .70 diagn e only, n 1.6 5. 12 the purification of proteins. Vivapure ostic pr ot to be 3 .06 0.60 oced use Store a ures. d for in 1.67 4 13.2 0 t room vitro 3 .50 refr tempera 1.7 3 14 igerate a ture (20 4 .3 .40 0.40 t +4˚C. ˚C - 25˚C 1 ) or .87 2.6 15.03 0.30 1 .94 1.3 15.66 0.25 2.0 0.6 16.83 0.20 0 17.46 0.10 purification protocols allow the 18.0 0.05 0.00 isolation of pure protein in less than 30 minutes.

Elute

it se K Vivapurerpo l Pu k era boo Gen and axi n H & M atio ini rific e M Pu pur tein Viva Pro

Pure protein

42 Vivapure Ion Exchange Spin Columns

Vivapure ion exchange spin columns come in either strong or weak cation or anion charged membrane matrices. With these ion + exchange membrane devices, protein binding, elution, and + concentration is made almost as + simple as filtration. + A + + + + +

C + +

Membrane availability B Functional groups: Ion exchanger type: - + There are three sizes of Vivapure IEX Sulphonic acid (S) Strong acidic cation exchanger: R-CH2-SO3 Na devices and different capacities. + - Quaternary ammonium (Q) Strong basic anion exchanger: R-CH2-N -(CH3)3Cl Carboxylic acid (C) Weak acidic cation exchanger: R-COO- A.Vivapure Mega - 75 ml + Binding capacity: High - H Diethylamine (D) Weak basic anion exchanger: R-CH2-NH -(CH2H5)2 B. Vivapure Maxi - 19/20 ml Typical Performance Binding capacities: Vivapure spin columns Protein binding Max. volume per Max. volume per High - H, Medium - M capacity* (mg) centrifuge run using centrifuge run using C. Vivapure Mini - 400/500 µl a swing-out rotor (ml) a fixed angle rotor (ml) Binding capacities: Vivapure Mini L 0.02 0.5 0.5 High - H, Medium - M, Low - L Vivapure Mini M 1 0.5 0.5 Vivapure Mini H 4 0.4 0.4 The Vivapure Advantage Vivapure Maxi M 15 - 20 20 10.5 • Fast spin columns Vivapure Maxi H 60 - 80 19 10.5 • Easy and ready to use Vivapure Mega H 180 - 230 75 • Scalable from µg to >200 mg Vivawell 96 0.200 0.8 Vivawell 8-Strip 1 0.3

Vivapure IEX in Multiwell Format *Actual yields depend on specific protein sample and selected pH and salt conditions. Yields established using 1 mg/ml BSA in 25 mM Tris/HCL pH 8.0 with Vivapure Q & D spin columns and 1 mg/ml cytochrome c in 25 mM sodium acetate Vivapure membrane adsorbers are buffer pH 5.5 with Vivapure S & C spin columns. also available as Vivawell 96-well and Vivawell 8-strip devices for increased Typical Applications throughput and robotic applications. • Fractionation prior to further analysis e.g. 2D gels • Scouting purification conditions for new protein preparation protocols • Endotoxin removal • Polishing His-tagged proteins after metal chelate chromatography • Purification and concentration of proteins • Removal of heme moiety from heme containing proteins

43 Vivapure Ion Exchange Ordering Codes

Cat Number Description Spin Columns Centrifuge Tubes Kits available Vivapure Mini Ion Exchange Spin Columns (up to 0.5 ml) VS-IX01ST16 Vivapure Mini H starter kit (4 of each ion exchange class) 16 32 VS-IX01CL24 Vivapure C Mini L 24 48 VS-IX01CM24 Vivapure C Mini M 24 48 VS-IX01CH24 Vivapure C Mini H 24 48 VS-IX01DL24 Vivapure D Mini L 24 48 VS-IX01DM24 Vivapure D Mini M 24 48 VS-IX01DH24 Vivapure D Mini H 24 48 VS-IX01QL24 Vivapure Q Mini L 24 48 VS-IX01QM24 Vivapure Q Mini M 24 48 VS-IX01QH24 Vivapure Q Mini H 24 48 VS-IX01SL24 Vivapure S Mini L 24 48 VS-IX01SM24 Vivapure S Mini M 24 48 VS-IX01SH24 Vivapure S Mini H 24 48 Vivapure Maxi Ion Exchange Spin Columns (up to 20 ml) VS-IX20CM08 Vivapure C Maxi M 8 16 VS-IX20CH08 Vivapure C Maxi H 8 16 VS-IX20DM08 Vivapure D Maxi M 8 16 VS-IX20DH08 Vivapure D Maxi H 8 16 VS-IX20QM08 Vivapure Q Maxi M 8 16 VS-IX20QH08 Vivapure Q Maxi H 8 16 VS-IX20SM08 Vivapure S Maxi M 8 16 VS-IX20SH08 Vivapure S Maxi H 8 16 Vivapure Mega Ion Exchange (up to 75 ml) VS-IX75CH02 Vivapure C Mega H 2 2 VS-IX75DH02 Vivapure D Mega H 2 2 VS-IX75QH02 Vivapure Q Mega H 2 2 VS-IX75SH02 Vivapure S Mega H 2 2 Vivapure Mega Accessories & Accessories for Air Pressure Mode VS-IXA01 Vivapure Mega pressure cap with 2 santoprene seals 1 unit VS-IXA02 Santoprene seals for Vivapure Mega 1 unit VSA003 250 ml centrifuge bottles with standard lids 4 units VCA002 Air Pressure Controller (APC) 1 unit VCA010 Female coupling 1 unit VCA011 Male coupling 1 unit VCA012 4 mm outer diameter pressure tubing (3 m) 1 unit Vivapure Kits VS-IX01QHGP Acidic Protein Purification Kit Q Mini H 8 32 VS-IX01SHGP Basic Protein Purification Kit S Mini H 8 32 VS-IX20QHGP Acidic Protein Purification Kit Q Maxi H 4 16 VS-IX20SHGP Basic Protein Purification Kit S Maxi H 4 16 VS-IX01DMDR DNA Removal Kit D Mini M 12 36 VS-IX20DMDR DNA Removal Kit D Maxi M 6 18 Vivawell Plates & 8-Strip Pack Size VW96IC02 Vivawell 96 Well Plate C 2 VW96ID02 Vivawell 96 Well Plate D 2 VW96IS02 Vivawell 96 Well Plate S 2 VW96IQ02 Vivawell 96 Well Plate Q 2 VW08IC02 Vivawell 8-Strip C 2 VW08ID02 Vivawell 8-Strip D 2 VW08IS02 Vivawell 8-Strip S 2 44 VW08IQ02 Vivawell 8-Strip Q 2 Vivapure® Metal Chelate Spin Columns

For customizing His-tagged* protein purification conditions

A new generation of Immobilized Metal Affinity Chromatography (IMAC) systems based on membrane adsorber technology. The Vivapure metal chelate columns are prepacked with a membrane containing covalently bound IDA groups (iminodiacetic acid). These groups are loaded with the metal ion of choice to form functional chelates that strongly and specifically bind the target His- tagged protein.

Available in 3 different sizes (Mini, Ordering Information Kit Contents Maxi and Mega) to cover a broad VS-MC01MC12 Vivapure Metal Chelate Mini spin columns range of binding capacities and Metal Chelate spin columns 12 sample volumes, Vivapure metal Clarification tubes 12 chelate columns are optimal for Centrifuge tubes 36 protocol optimisation and subsequent VS-MC20MH04 Vivapure Metal Chelate Maxi spin columns up-scaling. Metal Chelate spin columns 4 Clarification tubes 4 Vivawell-96 (96-well plate) and Centrifuge tubes 16 Vivawell 8-strips are the formats of VS-MC75MH02 Vivapure Metal Chelate Mega spin columns choice for high-throughput Metal Chelate spin columns 2 applications and for rapid protocol Centrifuge bottles (250 ml) 2 optimisation. Both systems use a VW96MC02 Vivawell-96 Metal Chelate standard microtiter plate footprint and 96-well protein purification plates 2 fit into swing-out rotors for deep-well VW08MC02 Vivawell 8-strip Metal Chelate plates. In addition, Vivawell 96-well is 8-strip protein purification strips 24 compatible with vacuum manifolds.

Typical Performance The Vivapure Advantage Vivapure Metal Chelate Protein binding Max. volume per • Flexible for protocol optimisation spin columns capacity (mg) centrifuge run (ml) • Centrifugal format Mini spin columns 0.4 0.4 • Fast and easy handling Maxi spin columns 6 - 8 19 • Parallel processing Mega spin columns 18 - 20 75 • Reproducible results MC Vivawell 96 0.03/well 0.8/well MC Vivawell 8-Strip 0.35/well 0.3/well

* protected by patents of third parties 45 Vivapure 8-to-96 well Cobalt-Chelate kit - for His-tagged* Protein Purification

Innovative modular system for high-throughput purification

The Vivapure 8-to-96 well cobalt- chelate kit is a unique tool for high- throughput purification of His-tagged proteins through Immobilized Metal Affinity Chromatography (IMAC). The modular 8-strip format enables flexible and parallel handling of different sample numbers, using either a multichannel device or an automated workstation. Multiple sample processing is accomplished by fast centrifugation-driven steps.

Hardware for each step is included in Ordering Information Kit Contents the kit: Vivaclear plus 8-strips for VS-HT08CC02 Vivapure 8-to-96 well cobalt-chelate kit lysate filtration, ready-to-use Vivapure Vivapure cobalt-chelate 8-strips 24 cobalt-chelate (iminodiacetic acid, IDA) Vivaclear plus 8-strips 24 8-strips for protein binding and 96- 96-well collection plates 8 well collection plates with seals for Seals 2 sample collection/storage.

Specifications Ideal for screening expression clones Binding capacity 250 µg (native or denaturing conditions), Maximum volume 500 µl optimising expression protocols, and Minimum elution volume 150 µl HT purification of proteins to be used in structural and functional studies or for spotting protein arrays.

The Vivapure Advantage • Ready-to-use • Modular format • Easy to handle • Fast centrifugation steps • Robot-friendly

46 Vivapure Protein A Kit

Antibody purification in rapid centrifugal steps

Vivapure Protein A Mini spin columns are innovative membrane based devices for rapid purification of antibodies. Using the Vivapure adsorber technology, the columns are prepacked with a membrane to which Protein A molecules are coupled, thus assuring highly specific recoveries of ultrapure antibodies.

Vivapure Protein A spin columns are the size of standard microcentrifuge tubes for quick and parallel handling. Purification of multiple antibody Ordering Information Kit Contents samples is achieved in a few VS-PA01PA24 Vivapure Protein A Kit centrifugal steps making the antibody Vivapure Protein A Mini spin columns 24 purification process as easy as Centrifuge tubes 72 filtration.

Specifications Due to their small scale capacity and Binding capacity 300 µg easy handling, Vivapure Protein A Mini Maximum volume 400 µl spin columns are an ideal tool for Minimum elution volume 400 µl screening purposes.

The Vivapure Advantage • Centrifugal format • Fast and easy handling • Parallel processing • High purity

Handling overview

Original Antibody is Wash Elute Pure antibody sample bound to membrane

47 Vivapure Epoxy Protein Coupling Kit

Coupling affinity ligands with epoxy groups

The Vivapure Epoxy Protein Coupling Kit is a new tool for protein immobilization for creating any desired affinity spin column.

This ready-to-use kit utilizes an epoxy membrane adsorber that is fitted into a spin column for easy and quick handling with optimized buffers to couple proteins covalently in three hours.

Sample handling in spin columns allow small working volumes. With Vivapure Ordering Information Kit Contents spin columns, all steps can be carried VS-PC01EPPC Vivapure Epoxy Protein Coupling Kit out in a centrifuge making protein Vivapure Epoxy Mini spin columns 12 coupling and the following purification Vivaspin 500, 10 kDa MWCO 12 procedure as easy as ultrafiltration. Clarification Mini spin columns 12 Collection tubes (2 ml) 36 Immobilization of proteins Buffer A 25 ml Amino acids possessing either amino-, Buffer B 25 ml hydroxyl- or thiol-groups can be immobilized with the epoxy group. Specifications Coupling Capacity 0.2 - 0.5 mg The Vivapure Advantage Volume per centrifuge run 100 - 400 µl • Fast results - only 3 hours coupling Elution volume (antigen purification) 100 - 400 µl • Mild coupling conditions Recommended centrifugation speed 400 x g - 12,000 x g (please refer to protocol) • Ready-to-use kit

Handling overview

Custom Dilute 1:1 Equilibrate Load Diluted flow-through Pipette flow- Wash Custom protein Protein with Buffer A Membrane Protein sample through onto coupled to Sample membrane; Vivapure Epoxy incubate 3 hr Mini Spin Column at RT

48 Vivapure Anti-HSA/IgG Kits - for Human Albumin and Human Albumin/IgG Depletion

The Vivapure Anti-HSA and Anti- HSA/IgG kits are intended for biologists involved in the discovery of Before serum biomarkers that need highly specific albumin or albumin and IgG removal at single use pricing.

The Vivapure Albumin Depletion Kit is based on a unique antibody fragment for specific albumin removal. After*

The Albumin/IgG Depletion Kit uses a combination of the Anti-HSA antibody fragment and Protein G resin for *HSA and IgG Removal depleting albumin and IgG.

Additionally, all buffers and spin tubes Ordering Information Kit Contents required for albumin and albumin/IgG VS-SP08HAR Vivapure Anti-HSA Kit for Human Albumin Depletion removal from 12 x 20 µl samples of Anti-HSA Affinity Resin (50% slurry) 5 ml human serum are included as well as Clarification spin columns (Vivaclear) 12 a recommended protocol for recovery Collection tubes (2 ml) 24 of albumin or albumin and IgG and Binding Buffer 15 ml associated proteins. VS-SP50HAR Vivapure Anti-HSA Affinity Resin for Human Albumin Depletion Anti-HSA Affinity Resin (50% slurry) 50 ml The Vivapure Advantage VS-SP08HAIGG Vivapure Anti-HSA/IgG Kit for Human Albumin and IgG Depletion • Highly specific antibody fragment Anti-HSA/IgG Affinity Resin (50% slurry) 5.5 ml based albumin removal Clarification spin columns 12 • Protein G based IgG removal Collection tubes (2 ml) 24 • Priced for single use - no risk of Binding Buffer 15 ml contamination Specifications: Vivapure Anti-HSA and Anti-HSA/IgG Kits Anti-HSA Affinity Resin binding capacity 2 mg/ml (suspension containing 50% packed medium) Anti-HSA/IgG Affinity Resin binding capacity 1.8 mg/ml albumin (suspension containing 50% packed medium) 0.6 mg/ml IgG Clarification spin columns (Vivaclear) max. volume capacity 500 µl Recommended centrifugation speed 400 x g

Handling overview - Albumin and Albumin/IgG removal in 20 minutes

Add up to 20 µl serum SPIN SPIN sample

Fill Vivaclear Mix sample with Anti-HSA/IgG Recover albumin Wash 1 - 3x with Binding Buffer for with Anti-HSA/IgG Affinity Resin incubate for and IgG depleted sample complete sample recovery Albumin and IgG depleted Affinity Resin 15 min. sample is ready for analysis 49 Vivapure C18 Micro Spin Columns

Fast sample preparation for mass spectrometry

Vivapure C18 Micro spin columns are centrifugal membrane-based devices for concentration, purification and desalting of peptides prior to analysis by mass spectrometry. The columns are prepacked with a membrane containing C18 hydrophobic chains for reversed-phase affinity chromatography.

The columns are the size of standard microcentrifuge tubes. With Vivapure C18 micro spin columns, a few centrifugation steps replace the Ordering Information Kit Contents tedious repetitive pipetting procedure VS-RP218L24 Vivapure C18 Micro spin columns for sample preparation prior to MALDI Vivapure C18 Micro spin columns 24 MS analysis. Samples are easily Micro collection tubes (200 µl) 24 processed in parallel, and the tiny Collection tubes (2 ml) 48 elution volumes are thoroughly collected in the included microtubes. Specifications Binding capacity (for standard digestion) 5 µg The Vivapure C18 spin columns offer a Maximum volume 200 µl very fast and effective method to Minimum elution volume 3 µl simultaneously desalt and concentrate up to 200 µl of highly The Vivapure Advantage dilute peptide solutions from any • Centrifugal format source (2D-PAGE, chromatographic • High volume capacity methods or biological samples). • Low elution volume • Parallel processing • High reproducibility • Elution in Matrix

Handling overview

SPIN SPIN SPIN SPIN

Equilibrate Bind Wash Elute Purified sample 50 Vivapure AdenoPACK™ 100 - for Adenovirus purification and concentration

The AdenoPACK 100 adenovirus purification and concentration kit is intended for researchers who need to recover a range of 1 - 5 x 1012 purified virus particles in a milliliter for in-vitro transfections. The kit includes all reagents and devices necessary for clarification, purification and concentration of Adenovirus Typ 5 from HEK293 cell cultures.

The rapid two-hour purification is made possible by use of Vivascience’s membrane adsorber technology, unique in its capability to efficiently capture and recover large virus particles. Ordering Information Kit Contents VS-AVPQ101 Vivapure AdenoPACK 100 Convective flow through the AdenoPACK Membrane Adsorber 2 AdenoPACK syringe filter devices Benzonase®* 12.5 U/µl 120 µl provides high-speed separations not Minisart Plus 4 possible with chromatography and 20 ml Syringe 4 cesium chloride density gradients. Tubing set and one way vale 1 10 ml syringe (elution) 2 The Vivapure Advantage Elution Tip (Red) 2 • Rapid 2 hour purification protocol 12 Loading Buffer 1 x 120 ml • Fast recovery of 1 - 5 x 10 VP Washing Buffer 1 x 120 ml from 100 ml cell culture Elution Buffer 1 x 20 ml • Easy to use Vivaspin 15R concentrator 4 • Non-toxic buffers Instructions (for each Kit and Vivaspin) 1 • Complete kit including Minisarts, *Benzonase® is a trademark of Merck KG aA, Darmstadt, Germany. Benzonase® must be stored at -20°C upon receipt Vivaspins and Buffers Kit Specifications Sample Size 20 to 100 ml of Adenovirus supernatants Virus particles (VP) per ml Typically 1 - 5 x 1012 VP/IU 20 - 50 Processing time Typically 2 hours

Handling Overview - Purified virus in about 2 hours

A. B. C. D. E.

Purified virus in about 2 hours

Add Benzonase® Load cleared Wash Elute Concentrate 51 and clarify viral viral supernatant supernatant Filtration Products

Minisart® 0.2 µm Syringe Filter Holders for Rapid Small Volume Sterilization with Maximum User Convenience Ready-to-use units, which offer high flow rates at low inlet pressures, make an correspondingly rapid sterile filtration possible. Fitted on a standard syringe, they enable a less hand-tiring sterilization of up to 100 ml of liquid. A Minisart fitted on a standard dosing syringe makes up a very convenient system for simultaneous dosing and sterilization.

Minisart plus units are of advantage for the sterilization of difficult to filter liquids. They include a fine glass fibre prefilter on the filter membrane, a combination which is so effective that throughputs can often be doubled. Minisarts and their packaging are environmentally friendly, free of PVC!

Ordering Information 0.2 µm Minisart, CA membrane 16534 K Pack of 50, sterile, individually packed, with luer lock outlet 17597 K Pack of 50, sterile, individually packed, with male luer lock outlet 16534 Q Pack of 500, non-sterile bulk packed, with luer lock outlet 17597 Q Pack of 500, non-sterile bulk packed, with male luer slip outlet 0.2 µm Minisart plus with GF Pre-filter 17823 K Pack of 50, sterile, individually packed, with luer lock outlet 17823 Q Pack of 500, non-sterile bulk packed, with luer lock outlet

52 Filtration Products

High Flow Rate Minisart® Syringe Filters for Particle Removal, Ultracleaning and Prefiltration

Ready-to-use filter units with 0.45 µm, Ordering Information 0.8 µm, 1.2 µm or 5 µm pore size Minisart high flow, PES membrane membrane filters. These independent 16553 K 0.1 µm, pack of 50, sterile, filters fulfill your filtration individually packed with luer lock outlet requirements whenever volumes of up 16532 K 0.2 µm, pack of 50, sterile, to 100 ml must be clarified or ultra- individually packed with luer lock outlet cleaned. They can also be used as 16541 K 0.2 µm, pack of 50, sterile, prefilter in combination with a 0.2 µm individually packed with luer slip outlet Minisart, increasing the total filterable 16537 K 0.45 µm, pack of 50, sterile, volume. individually packed with luer lock outlet 16533 K 0.45 µm, pack of 50, sterile, The high flow rates of these units individually packed with luer slip outlet result from the large filter area and the Standard 0.45 µm to 5 µm Minisarts, CA membrane very low flow resistance of the filter 17598 K Pack of 50, 0.45 µm with luer slip outlet, support, which is demonstrated by the sterile, individually packed relative constant increase in the flow 16555 K 0.45 µm Minisart with luer lock outlet rate with increasing pore size. 16592 K 0.8 µm Minisart with luer lock outlet 17593 K 1.2 µm Minisart with luer lock outlet Minisart GF contains a glass fiber filter 17594 K 5 µm Minisart with luer lock outlet with a retention efficiency of 98% for 17598 Q Pack of 500, 0.45 µm with luer slip outlet, non-sterile 0.7 µm spherical particles. It is very 16555 Q 0.45 µm Minisart with luer lock outlet useful when relatively dirty solutions 16592 Q 0.8 µm Minisart with luer lock outlet are to be clarified, or as a prefilter is 17593 Q 1.2 µm Minisart with luer lock outlet needed on an 0.2 µm or 0.45 µm 17594 Q 5 µm Minisart with luer lock outlet Minisart. Minisart plus units also Minisart plus with GF Pre-filter contain this glass fiber filter, but as 17829 K Pack of 50, 0.45 µm with luer lock outlet, prefilter on an 0.45 µm or 1.2 µm sterile, individually packed membrane, for higher total 17829 Q Pack of 500, 0.45 µm with luer lock outlet, non-sterile throughputs. 17825 Q 1.2 µm with luer lock outlet Minisart GF 17824 K Pack of 50, non-sterile, with luer lock outlet 17824 Q Pack of 500, with luer lock outlet

53 Filtration Products

Minisart® SRP Units with a Clean and Chemically Inert PTFE Membrane

Ready-to-use units for simple,rapid Ordering Information PTFE and reliable ultracleaning of small- Minisart SRP4 volume samples for the HPLC or GC 17820 K With 0.45 µm membrane, pack of 50 analysis, which require an even more 17820 Q With 0.45 µm membrane, pack of 500 chemical resistant unit than Minisart Minisart SRP15 with spike outlet RC, e.g: for solvents such as acetone, 17558 K With 0.2 µm membrane, pack of 50 dimethylformamide and DMSO, or for 17558 Q With 0.2 µm membrane, pack of 500 aggressive aqueous liquids. 17559 K With 0.45 µm membrane, pack of 50 17559 Q With 0.45 µm membrane, pack of 500 Minisart SRP4 is recommended for Minisart SRP15 with luer outlet sample volumes of up to about 1 ml, 17573 K With 0.2 µm membrane, pack of 50 Minisart SRP15 for up to about 5 ml 17573 ACK With 0.2 µm membrane, pack of 50, and Minisart SRP 25 for up to 100 ml. sterile, individually packed 17573 Q With 0.2 µm membrane, pack of 500 17574 K With 0.45 µm membrane, pack of 50 17574 Q With 0.45 µm membrane, pack of 500 Minisart SRP25 17575 K With 0.2 µm membrane, pack of 50 17575 ACK With 0.2 µm membrane, pack of 50, sterile, individually packed 17575 Q With 0.2 µm membrane, pack of 500 17576 K With 0.45 µm membrane, pack of 50 17576 Q With 0.45 µm membrane, pack of 500

Minisart® RC Units with Hydrophilic, Solvent-resistant RC-membranes

Ready-to-use syringe filter units for Ordering Information Regenerated Cellulose simple, rapid and reliable ultracleaning Minisart RC4 of small-volume samples for the HPLC 17821 K With 0.2 µm membrane, pack of 50 or GC analysis. 17821 Q With 0.2 µm membrane, pack of 500 17822 K With 0.45 µm membrane, pack of 50 Minisart RC4 is recommended for 17822 Q With 0.45 µm membrane, pack of 500 sample volumes of up to about 5 ml Minisart RC15 and Minisart RC25 for sample volumes 17761 K With 0.2 µm membrane, pack of 50 of up to about 100 ml. 17761 ACK With 0.2 µm membrane, pack of 50, sterile, individually packed Minisart RC units outperform 17761 Q With 0.2 µm membrane, pack of 500 competitive hydrophilic units in terms 17762 K With 0.45 µm membrane, pack of 50 of compatibility with aqueous 17762 Q With 0.45 µm membrane, pack of 500 solutions and solvent mixtures. Minisart RC25 17764 K With 0.2 µm membrane, pack of 50 17764 ACK With 0.2 µm membrane, pack of 50, sterile, individually packed 17764 Q With 0.2 µm membrane, pack of 500 17765 K With 0.45 µm membrane, pack of 50 17765 Q With 0.45 µm membrane, pack of 500 Minisart NY25, Nylon Membrane 17845 ACK With 0.2 µm membrane, pack of 50, 54 sterile, individually packed 17846 ACK With 0.45 µm membrane, pack of 50, sterile, individually packed Filtration Products

Sartolab® P20 and Sartolab® P20 plus for Reliable Sterile Filtration of Tissue Culture Solutions

Ready-to-use units which,attached to Ordering Information a membrane pump or tube pump, Sartolab P20 units, CA membrane, 0.2 µm quickly and reliably sterilize 100 ml to 18052 D With hose nipple inlet connection, pack of 10 5 liter of media and aqueous solutions. 18053 D With luer lock inlet connection, pack of 10 Sartolab P20 plus units The combination of a large filtration 18056 D With hose nipple inlet connection, pack of 10 2 area (20 cm ) and an automatic 18058 D With luer lock inlet connection, pack of 10 de-aeration ensures high flow rates 17576 Q With 0.45 µm membrane, pack of 500 and optimal total throughputs. Automatic venting of any trapped air through the PTFE membrane- protected vent ports ensures that the entire filter surface is used for effective filtration.

Sartolab P20 units have an extraordinarily favourable price- performance ratio. Often, the total filterable volume can even be doubled due to an integrated binder-free glass fibre prefilter.

55 Sartolab RF/BT & 150V Disposable Vacuum Filtration Units

Sartorius RF and BT units are Sartorius Sartolab 150V filtration optimised for the application in cell unit with pleated 0.2 µm PES culture. The built-in membrane made membrane for vacuum filtration sterile of polyethersulfone guarantees filtration of up to several liters. By extremely high flow rates and low opening the drain valve protected by a protein binding, and is therefore ideal 0.2 µm PTFE membrane, the created for the filtration of protein containing vacuum can be interrupted for solutions. replacing the filled receiver flask through a new one. Filtration restarts, The receiver flask is delivered with when the drain valve is getting closed. tube adapter and closure lid. This procedure of the “continuous” filtration can be repeated several The Sartolab RF units are sterile times. complete units with drainage vessel; the Sartolab BT holders can be The 0.2 µm pleated PES membrane adapted on usual in trade, vacuum with an area of 150 cm2 guarantees resistant bottles with a screw reliable sterile filtration of media, connector 45. Attention: Only use buffer and many other solutions. They bottles which are licensed for can be universally used on bottles subpressure methods. with a diameter of up to 58 mm.

Available in different sizes.

Ordering Information Sartolab RF vacuum filtration units 18081-E* Volume 150 ml, including receiver flask, pack of 12 18082-E* Volume 500 ml, including receiver flask, pack of 12 18083-E* Volume 1000 ml, including receiver flask, pack of 12 Sartolab BT vacuum filtration units 18084-E* Volume 150 ml, pack of 12 18085-E* Volume 500 ml, pack of 12 18086-E* Volume 1000 ml, pack of 12 For further information about Sartolab Sartolab 150 V RF/BT and 150V Vacuum Filtration units, visit our website at 18080-M Vacuum filtration unit, pack of 3 www.vivascience.com *Sartolab BT/RF can not be sold in: Australasia, Canada, Korea, Japan, Mexico or USA.

56 Biolab Instruments

arium® Laboratory Water Systems

The Sartorius arium® laboratory water systems feature a range of models to meet laboratories’ needs for purified water. Whether it’s RO water you require for general buffer preparation or Ultrapure 18.2 MΩ water, Sartorius has a system for you.

BIOSTAT®er - Economy Fermenter - Bioreactor System

Your Start in Fermentation and Cell Cultivation. The BIOSTAT®er is a compact autoclavable laboratory fermenter / bioreactor system.

Certomat® BS-1

Fully programmable, high-capacity incubation shaker, stackable up to three units.

Mikro-Dismembrator S

High performance ball mill homogenizer, time and speed electronically controlled.

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Specifications subject to change without notice. Publication No.: SLU0001-e05038 Order No.: 85030-516-27