United States Patent (19) (11) 4,111,774 Landis 45) Sep

Total Page:16

File Type:pdf, Size:1020Kb

United States Patent (19) (11) 4,111,774 Landis 45) Sep United States Patent (19) (11) 4,111,774 Landis 45) Sep. 5, 1978 54 ELECTROFILTRATION OF ANIMAL FATS Attorney, Agent, or Firm-Sidney B. Ring; Hyman F. (75) Inventor: Joel Victor Landis, Houston, Tex. Glass 73 Assignee: Petrolite Corporation, St. Louis, Mo. 57 ABSTRACT This invention relates to the electrofiltration of ren (21) Appl. No.: 768,002 dered animal fat. By means of this process, foreign bod ies present in said fat are facilely removed without the 22 Filed: Feb. 11, 1977 conventional steps of water washing, centrifugation and 51 Int. C.’................................................ B03C5/00 drying. The use of electrofiltration instead of water 52 U.S. C. ..................................... 204/186; 204/131 washing, centrifugation and drying, besides avoiding 58 Field of Search ............... 204/186, 302, 167, 149, the operation steps, also minimizes hydrolysis of fats to 204/152, 131, 180 R fatty acids. In addition, the physical loss of fat, which usually occurs during washing and subsequent steps, is (56) References Cited minimized; and impurities not conventionally removed U.S. PATENT DOCUMENTS by water washing are removed, such as trace metals, 1,162,213 11/1915 Bloom .................................. 204/186 etc. The electrofiltered product is a refined lard or 1,326,968 1/1920 Rogers 204/131 tallow which is superior to lard or tallow prepared by 3,394,067 7/1968 Shirley 204/186 conventional procedures. 3,928, 158 12/1975 Fritsche ............................... 204/302 Primary Examiner-T. M. Tufariello 8 Claims, 1 Drawing Figure waZaA prior A77 - SMG APEAIAWEd Aa AA/wed AaT AwaAED AAAAIMG Aat AAASeay7 Apocass U.S. Patent Sept. 5, 1978 4,111,774 – | | | | | | | - |-----—————————————— |× ——————————————— 4,111,774 2 tion. The electric field in the bed of particulate solids is ELECTROFILTRATION OF ANMAL FATS periodically interrupted and then, a dielectric fluid is Animal fats, such as that derived from cattle and circulated therethrough to remove the priorly adhering hogs, etc., are rendered from fatty stocks by a variety of finely divided solid material. methods. Typical examples are the so-called Dry and 5 The difference between the "prior art” and the “pres Wet Rendering Process. ent process” is graphically presented in the drawing. Dry Rendering is distinguished from Wet Rendering The electrofilter 1 is a metal pressure vessel 2 contain in that the dehydration of fat is accompanied by dehy ing a porous bed of dielectric particulate solids 3 dis dration of fat and fatty tissues so that the latter is essen posed in an intense d.c. electric field so that the solid tially dry at the end of the operation. O materials in the fat are substantially removed by their Dry Rendering is preferred for inedible products induced adherence to the particulate solids. The elec where flavor and odor are secondary and the produc trofilter 1 can be of commercial design sold in the mar tion of large quantities of high quality residues is impor ketplace under the designation Petreco (R) Electro-Fil tant. ter'Separator. The electrofilter 1 has an inlet 4 and an Wet Rendering is generally used for edible products 15 outlet 5. The fat flows from the inlet 4 into a distributor where color, flavor and keeping quality are of prime 6 provided by pipe crossarms containing metering importance and the relative residue is small. There are openings. The interior of the vessel contains a plurality two varieties of Wet Rendering - low temperature of energized electrodes 7 in spaced relationship to a rendering up to the boiling point of water and high plurality of internested grounded electrodes 8. Prefera temperature steam rendering under pressure in closed 20 bly, the electrodes 7 and 8 are vertically elongated vessels. metal tubes that have substantial overlapping dimen Most of the animal fat produced in the United States sions defining an electric field within the particulate is rendered by the steam process. Thus in most instances solids 3. The electrodes 7 are suspended from the vessel lard, tallow or fish oil such as whale oil are usually by insulators 9. In addition, the electrodes 7 are ener steam rendered. 25 gized by an external power supply 10 having a connec Tallow or lard, thus rendered, contains foreign bodies tion to an a.c. power source and providing a high inten such as proteins, etc., and must be treated to yield re sity potential through a conductor 11, an entrance bush fined lard or tallow. Refining is carried out by treatment ing 12 and a flexible lead 13 to the electrodes 7. The with a hot water washing (with or without "Fuller's' power supply 10 can be grounded to the vessel 2 by the earth) followed by centrifugation and drying. In the 30 conductor 14 so that the vessel 2 provides an additional course of refining, the acid number of the fat is in grounded electrode. creased and certain amounts of fats are physically lost. The d.c. electric field induces the tenacious adhesion in addition, certain materials present in the fats are not of the finely divided solid material upon the particulate removed by water washing, such as metals, etc. This solids 3. The power supply 10 should provide a suffi process is designated "Prior Art” in the Drawing. 35 cient intensity d.c. electric field within the particulate I have now discovered a process of refining rendered solids 3 contained within the electric field defined by edible animal fat which is characterized by the elec the electrodes 7 and 8. Preferably, the electric field trofiltration of such edible animal fat in liquid form. By produces a potential gradient in the particulate solids 3 means of this process, foreign bodies present in the fat of not less than about 20 kilovolts per inch. A certain are facilely removed without the necessity of water 40 type of particulate solids should be employed for opti washing, followed by centrifugation and drying of the mum results. Preferably, the solids 3 are composed of a water washed fat. The use of electrofiltration instead of rigid solid material having a relatively low dielectric water washing, centrifugation and drying, besides constant (e.g., below about 6 at 1 kilohertz). More par avoiding the operational steps, also minimizes the hy ticularly, the particulate solid is chemically inert, in drolysis of fats to fatty acids. In addition, the physical 45 compressible, hard, granular and rigid in nature. The loss of fat which usually occurs during washing and particulate solids can be a solid mineral material con subsequent steps is minimized, and impurities not con taining crystalline silicon dioxide, such as flint, garnet, ventionally removed by water washing are removed, granite and fused quartz. Preferably, the mineral is such as trace metals, etc. The electrofiltered product is crushed to provide nonspheroidal configurations which a refined lard or tallow superior to lard or tallow pre 50 have relatively discontinuous surfaces. As shown in pared by conventional procedures. The Electrofiltra Example 1, crushed flint rock having particulate sizes tion Process is designated as "Present Process' in the with minimum dimensions between about one-eighth Drawing. and one-half inch is employed to good advantage as in The electrofilter will tolerate or remove a certain the present process. Glass, including glass beads, can amount of water from the animal fats. When the amount 55 also be employed as the particulate solid. of water in the animal fats poses an excess load on the The electrofilter i produces, for practical purposes, electrofilter, the rendered animal fat is dried prior to the substantially complete removal of the finely divided electrofiltration. solid materials. In addition, the electrofilter also re In accordance with this invention, there is provided a moves small amounts of entrained water. It appears also process for refining animal fats. The rendered animal fat that phosphatides, carbohydrates, and other nonglyce in liquid form is subjected to electrofiltration by passage ride extractives in some instances are removed concur through a bed of dielectric particulate solids interposed rently with the finely solid materials. The resultant within a d.c. electric field having a gradient of at least improvement in color and subsequently low refining 20 kilovolts per inch whereby the finely divided solid losses indicates that the electrofilter 1 does produce this material in substantial totality adheres to the particulate 65 removal of additional materials besides the finely di solids in the bed and provides refined animal fats that vided solid materials. are substantially free of finely divided solid material. Eventually the electrofilter 1 removes such large The refined animal fat is passed to a subsequent utiliza amounts of the finely divided solid material from the 4,111,774 3 4. animal fat that the electric field is no longer adequate to Furthermore, refining by washing involves an average produce refined animal fat. At this time, or some se fat loss during washing of about 0.8% by weight, which lected time period or by throughput volume, the elec not only adds to the cost of the fat but also poses a trofilter 1 is cleaned and restored to original operating pollution problem. efficiency. For this purpose, the power supply 10 is 5 It will be apparent that there has been provided a deactivated to terminate the d.c. electric field within the process for refining animal fats without the disadvan electrofilter 1. Then, a dielectric fluid is circulated tages of conventional processes. The present process is through the particulate solids 3 to remove the adhering completely compatible with conventional operations in finely divided solid material. Stated in another manner, the food industry or other places where fats are refined. without the electric field being present, the circulating 10 Of special advantage is the intimate combination and dielectric fluid removes all of the adhering finely di cooperation in the use of an electrofilter in purification vided solid materials from the particulate solids 3 within of animal fats to remove finely dispersed solid materials the electrofilter 1.
Recommended publications
  • Ultracentrifugation Techniques for the Ordering of Nanoparticles
    nanomaterials Review Ultracentrifugation Techniques for the Ordering of Nanoparticles Xufeng Xu 1,† and Helmut Cölfen 2,* 1 Laboratory of Physical Chemistry, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5612AE Eindhoven, The Netherlands; [email protected] 2 Physical Chemistry, University of Konstanz, Universitätsstraße 10, Box 714, 78457 Konstanz, Germany * Correspondence: [email protected] † Present address: Institute of Materials, École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland. Abstract: A centrifugal field can provide an external force for the ordering of nanoparticles. Especially with the knowledge from in-situ characterization by analytical (ultra)centrifugation, nanoparticle ordering can be rationally realized in preparative (ultra)centrifugation. This review summarizes the work back to the 1990s, where intuitive use of centrifugation was achieved for the fabrication of colloidal crystals to the very recent work where analytical (ultra)centrifugation is employed to tailor-make concentration gradients for advanced materials. This review is divided into three main parts. In the introduction part, the history of ordering microbeads in gravity is discussed and with the size of particles reduced to nanometers, a centrifugal field is necessary. In the next part, the research on the ordering of nanoparticles in analytical and preparative centrifugation in recent decades is described. In the last part, the applications of the functional materials, fabricated from centrifugation-induced nanoparticle superstructures are briefly discussed. Keywords: centrifugation; sedimentation; nanoparticle; concentration gradient; non-equilibrium process; superstructure; functional material Citation: Xu, X.; Cölfen, H. Ultracentrifugation Techniques for 1. Introduction the Ordering of Nanoparticles. 1.1. The Era of Microbeads in Gravity Nanomaterials 2021, 11, 333.
    [Show full text]
  • Fractionation of Proteins with Two-Sided Electro-Ultrafiltration
    Journal of Biotechnology 128 (2007) 895–907 Fractionation of proteins with two-sided electro-ultrafiltration Tobias Kappler¨ ∗, Clemens Posten University of Karlsruhe, Institute of Engineering in Life Sciences, Division Bioprocess Engineering, Kaiserstr. 12, Geb. 30.70, 76128 Karlsruhe, Germany Received 7 June 2006; received in revised form 22 December 2006; accepted 2 January 2007 Abstract Downstream processing is a major challenge in bioprocess industry due to the high complexity of bio-suspensions itself, the low concentration of the product and the stress sensitivity of the valuable target molecules. A multitude of unit operations have to be joined together to achieve an acceptable purity and concentration of the product. Since each of the unit operations leads to a certain product loss, one important aim in downstream-research is the combination of different separation principles into one unit operation. In the current work a dead-end membrane process is combined with an electrophoresis operation. In the past this concept has proven successfully for the concentration of biopolymers. The present work shows that using different ultrafiltration membranes in a two-sided electro-filter apparatus with flushed electrodes brought significant enhancement of the protein fractionation process. Due to electrophoretic effects, the filtration velocity could be kept on a very high level for a long time, furthermore, the selectivity of a binary separation process carried out exemplarily for bovine serum albumin (BSA) and lysozyme (LZ) could be greatly increased; in the current case up to a value of more than 800. Thus the new two-sided electro-ultrafiltration technique achieves both high product purity and short separation times.
    [Show full text]
  • Continuous-Flow Centrifugation to Collect Suspended Sediment for Chemical Analysis
    1 Table 6. Compounds detected in both equipment blank samples and not in corresponding source blank samples or at concentrations greater than two times the corresponding source blank sample concentration. Prepared in cooperation with the National Water Quality Monitoring Council and [Source data: Appendix A, table A1; Conn and Black (2014, table A4); and Conn and others (2015, table A11). CAS Registry Number: Chemical Abstracts Service Washington State Department of(CAS) Ecology Registry Number® (RN) is a registered trademark of the American Chemical Society. CAS recommends the verifi cation of CASRNs through CAS Client ServicesSM. Method: EPA, U.S. Environmental Protection Agency’s SW 846; SIM, select ion monitoring. Unit: µg/kg, microgram per kilogram; ng/kg, nanogram per kilogram. Sample type: River samples were from the Puyallup River, Washington. Q, qualifi er (blank cells indicate an unqualifi ed detection). J, estimated, result between the Continuous-Flow Centrifugationdetection level and reporting level; toNJ, result Collect did not meet all quantitation Suspended criteria (an estimated maxiumum possible concentration is reported in Result column) U, not detected above the reporting level (reported in the Result column); UJ, not detected above the detection level (reported in the Result column). Abbreviations: na, not Sediment for Chemicalapplicable; Analysis PCBs, polychlorinated biphenyls] Sample type Chapter 6 of CAS River River Commercial Commercial Section D, Water Quality Parameter name Registry Method Unit source equipment
    [Show full text]
  • Nanosep® Centrifugal Devices - Protocols for Use Nanosep® Centrifugal Devices - Protocols for Use
    Contact Us: www.pall.com/contact Nanosep® Centrifugal Devices - Protocols for Use Nanosep® Centrifugal Devices - Protocols for Use Ultrafiltration Fundamentals Purification and Handling of DNA Fragments PCR: Before and After Protein Purification and Handling Miscellaneous Protocols Appendices References Ordering Information Ultrafiltration Fundamentals Background Ultrafiltration (UF) is a membrane separation technique used to separate extremely small particles and dissolved molecules in fluids. The primary basis for separation is molecular size, although other factors such as molecule shape and charge can also play a role. Molecules larger than the membrane pores will be retained at the surface of the membrane (not in the polymer matrix as they are retained in microporous membranes) and concentrated during the ultrafiltration process. Compared to non-membrane processes (chromatography, dialysis, solvent extraction, or centrifugation), ultrafiltration: Is far gentler to the molecules being processed. Does not require an organic extraction which may denature labile proteins. Maintains the ionic and pH milieu. Is fast and relatively inexpensive. Can be performed at low temperatures (for example, in the cold room). Is very efficient and can simultaneously concentrate and purify molecules. The retention properties of ultrafiltration membranes are expressed as Molecular Weight Cutoff (MWCO). This value refers to the approximate molecular weight (MW) of a dilute globular solute (i.e., a typical protein) which is 90% retained by the membrane. However, a molecule’s shape can have a direct effect on its retention by a membrane. For example, linear molecules like DNA may find their way through pores that will retain a globular species of the same molecular weight. There are three generic applications for ultrafiltration: 1.
    [Show full text]
  • Centrifugal Hyperfiltration
    TO: Technology Innovation Program Mail Stop 4750 National Institute of Standards and Technology 100 Bureau Drive Gaithersburg, MD 20899-4750 FM: Dirk Forman 125 S. St. Louis Lafayette Louisiana 70506 [email protected] Centrifugal Hyperfiltration A means to economically recover resources from saltwater and wastewater streams with lower the energy cost of producing freshwater and potable water from marginalized water resources. Introduction Reverse Osmosis (RO) is a filtration process for the removal of ionic and organic pollutants from wastewater. Today’s technology of utilization of this filtration process is by large array of high-pressure piping and pressure pumps. This process yields low volumes of filtrated output (permeate), utilizes large areas for pipe array and components and the concentration polarization and membrane fouling hinders the wide application of RO filtration process. Utilizing centrifugal forces and cross flow membranes has potential to change the standard (RO) process to one of portable and high volume water purification. AN AREA OF CRITICAL NATIONAL NEED Ensuring Future Water Supply: As the Nation’s population and economy grow, greater demands are being placed on freshwater resources. At the same time, temporary or permanent drought conditions and water access rights affect regional freshwater availability. Water needs threaten to outstrip available freshwater, now and in the future. Emerging contaminants that must either be removed from distributed water or converted to harmless forms of waste is also pressuring water quality, both in terms of decontamination and disinfection of water supplies. Food contaminations are often traced back to water contaminations, either in the field or in processing. Municipal waste streams and irrigation runoff waste resources that are not recovered.1 Over 97 percent of the Earth's water -- seawater and brackish groundwater -- is too salty to use for drinking water or agriculture.
    [Show full text]
  • Removal of Waterborne Particles by Electrofiltration: Pilot-Scale Testing
    ENVIRONMENTAL ENGINEERING SCIENCE Volume 26, Number 12, 2009 ª Mary Ann Liebert, Inc. DOI: 10.1089=ees.2009.0238 Removal of Waterborne Particles by Electrofiltration: Pilot-Scale Testing Ying Li,1 Ray Ehrhard,1 Pratim Biswas,1,* Pramod Kulkarni,2 Keith Carns,3 Craig Patterson,4 Radha Krishnan,5 and Rajib Sinha5 1Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, Missouri. 2Center for Disease Control and Prevention, National Institute for Occupational Safety and Health, Cincinnati, Ohio. 3Global Energy Partners, LLC, Oakhurst, California. 4Office of Research and Development, National Risk Management Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio. 5Shaw Environmental and Infrastructure, Inc., Cincinnati, Ohio. Received: July 6, 2009 Accepted in revised form: October 20, 2009 Abstract Theoretical analysis using a trajectory approach indicated that in the presence of an external electric field, charged waterborne particles are subject to an additional migration velocity that increases their deposition on the surface of collectors (e.g., sand filter). Although researchers conducted bench-scale experiments to verify the effectiveness of electrofiltration, few studies have reported on the applications of electrofiltration in larger scale facilities. In this study, a prototype pilot-scale electrofiltration unit, consisting of an acrylic tank (0.3Â0.3Â1.2 m) with vertically placed stainless steel mesh electrodes embedded in a sand filter was tested at a local drinking water plant. Presedimentation basin water was used as the influent with a turbidity ranging from 12 to 37 NTU. At an approach velocity of 0.84 mm=s, an electrode voltage at 8 and 12 V increased the particle removal coefficient pC* [defined as Àlog(Cout=Cin)] to 1.79 and 1.86, respectively, compared to 1.48 when there was no electric field.
    [Show full text]
  • 404 Sedimentation Handout
    Sedimentation for Analysis and Separation of Macromolecules A ‘centrifugal force’ as experienced by particles in a centrifuge actually reflects the natural tendency of the particle to move in a straight line in the absence of external forces. If we view the experiment in the (fixed) reference frame of the laboratory, we observe that the particle is deflected from a linear trajectory by a centripetal force (directed toward the central axis of the rotor). However, when we consider the motion of the particle with respect to the (rotating) reference frame of the rotor, it appears that at any instant the particle experiences an outward or centrifugal force: Instantaneous Velocity (vtang) Centrifugal Acceleration Centripetal Acceleration As seen in laboratory As seen in (rotating) reference frame reference frame of rotor Ignoring buoyancy effects for the moment, the magnitude of the centrifugal force (which, in the rotating reference frame of the rotor, is as real as any other force) is m ⋅ v2 F = objecttan g =⋅mrω 2 centrif r object [1] where r is the distance from the axis of rotation, vtang is the linear velocity at any instant (in the direction perpendicular to the radial axis) and ω is the angular velocity (in radians sec-1 – note that 1 rpm = 2π///60 rad sec-1). The magnitude of this force can be compared to that of the gravitational force at the earth’s surface: Gm⋅()⋅() m = earth object Fgrav 2 re [2] -8 -1 3 -2 where G is the universal gravitational constant (= 6.67 x 10 g cm sec ) and re and me are the earth’s radius (= 6.37 x 108 cm) and mass (5.976 x 1027 g), respectively.
    [Show full text]
  • Guide to Gel Filtration Or Size Exclusion Chromatography
    Guide to Gel Filtration or Size Exclusion Chromatography www.harvardapparatus.com Table of Contents Introduction ........................................................................ 2-4 Size Fractionation .................................................................... 5 Buffer Sample Selection ........................................................ 6-7 Selection of Media and Size .................................................... 8 Gel Filtration SpinColumns .................................................... 9 Spehadex P-25 Applications .................................................. 10 Desalting Columns Applications ............................................ 11 P-2, P-6 and P-30 SpinColumns .......................................... 12 Ordering Information ............................................................ 13 Contact Information .............................................................. 14 Guide to Gel Filtration or Size Exclusion Chromatography 1 Introduction Gel Filtration Gel Filtration also called size-exclusion chromatography can be used for protein DNA purification, buffer exchange, desalting, or for group separation in which the sample is separated in two major groups. Gel Filtration is an easy to use method for separation of molecules with different molecular sizes, using mild conditions. Gel Filtration uses the size of molecules in solution to determine separation. SpinColumns have short media packing so the samples are separated by size, the large molecules travel out of the column with the void
    [Show full text]
  • Recent Developments in Centrifuge Technology Harald Anlauf ∗ Universit¨Atkarlsruhe (TH), Institut F¨Urmvm, D-76128 Karlsruhe, Germany
    First published in: Available online at www.sciencedirect.com Separation and Purification Technology 58 (2007) 242–246 Recent developments in centrifuge technology Harald Anlauf ∗ Universit¨atKarlsruhe (TH), Institut f¨urMVM, D-76128 Karlsruhe, Germany Abstract Centrifugation represents one of the main groups of mechanical particle-liquid separation processes. There are available various centrifuges for sedimentation and filtration, which are operating continuously or discontinuously. The tasks for centrifugal separation are very wide spread from liquid clarification and purification, particle thickening, solids demoistening, particle fractionating and sorting, solids washing, liquid–liquid separation to extraction of solids or liquids. Applications of centrifuges can be found in all kinds of industry, in environmental protection, water treatment, etc. In the centrifugal field mass forces are present during the separation process. This leads to specific advantages and disadvantages in comparison to competing separation processes. Research and development for centrifugal processes today has not coming to an end but several new ideas to use centrifugal forces for separation and new technical developments in this field can be observed. After description of some fundamentals and presentation of an overview of the technical variations some aspects of advantages and disadvantages of centrifuges in comparison to other separation techniques are discussed and examples for recent research and technical development as well as actual trends in the field of centrifugation are given. © 2007 Published by Elsevier B.V. Keywords: Solid–liquid separation; Centrifugation; Filtration; Sedimentation 1. Introduction like demonstrated in Fig. 3. Centrifuges are especially well suited to separate molecularly inmiscible liquids, whereby Centrifuges are representing one of the main techniques to frequently in addition solid particles are present.
    [Show full text]
  • Continuos Treatment of Oily Sludge at Colombian Refineries
    CONTINUOS TREATMENT OF OILY SLUDGE AT COLOMBIAN REFINERIES Víctor Echeverría*1, Gladys Monsalve2 and Humberto Vidales1 1Ecopetrol - Instituto Colombiano del Petróleo, A.A. 4185, Bucaramanga, Santander, Colombia (SA) e-mail: [email protected] e-mail: [email protected] 2Ecopetrol – Gerencia Complejo Industrial Barrancabermeja, A.A. 77605, Bucaramanga, Santander, Colombia (SA) e-mail: [email protected] (Received 22 April 2002; Accepted 15 October 2002) he Colombian Petroleum Institute –ICP–, the research and development branch of Ecopetrol has developed a unique technological package used to treat oily sludge in a continuous way. The sludge Tcomes from a refinery with a 220 000 barrels of crude per day load, located in the Middle Madgalena River Valley in Colombia. The Technological Package allows for :a) the recovery of the hydrocarbon contained in oily wastes (up to 50%); b) the elimination of the oil contained in solid using a biodegradation process; and, c) the availability of an economically and technically feasible solution to handle oily sludge generated in the refinery. The oily wastes treated in this process come from maintenance of refinery´s equipment and also from the physical- chemical separation process at the industrial waste water treatment plant. Oily sludge is a complex system where light and heavy oils, contaminated water and contaminated solids coexist in the form of direct, inverse and multiple emulsions. The comprehensive technological package al- lows the treatment of oily sludge in a cost-effective way. ICP´s Technological package developed includes technologies combining mechanical, thermal, chemical and electrostatic dehydration techniques, and stimulated and intensive bioremediation to decontamination of solids saturated with residual oil.
    [Show full text]
  • Precipitation and Crystallization Processes
    Precipitation and Crystallization Processes Gordon Jarvenin Los Alamos National Laboratory Introduction Precipitation and crystallization refer to unit operations that generate a solid from a supersaturated solution. The non-equilibrium supersaturated condition can be induced in a variety of ways such as removal of solvent by evaporation, addition of another solvent, changes of temperature or pressure, addition of other solutes, oxidation-reduction reactions, or even combinations of these. The distinction between precipitation and crystallization is quite often based on the speed of the process and the size of the solid particles produced. The term precipitation commonly refers to a process which results in rapid solid formation that can give small crystals that may not appear crystalline to the eye, but still may give very distinct x-ray diffraction peaks. Amorphous solids (at least as indicated by x-ray diffraction) may also be produced. The term precipitation also tends to be applied to a relatively irreversible reaction between an added reagent and other species in solution whereas crystallization products can usually be redissolved using simple means such as heating or dilution. Precipitation processes usually begin at high supersaturation where rapid nucleation and growth of solid phases occur. In both precipitation and crystallization processes the same basic steps occur: supersaturation, nucleation and growth. Nucleation does not necessarily begin immediately on reaching a supersaturated condition, except at very high supersaturation, and there may be an induction period before detection of the first crystals or solid particles. Nucleation can occur by both homogeneous and heterogeneous processes. In general, homogeneous nucleation is difficult to achieve because of the presence of heteronuclei from colloids, dust, or other foreign material in the solution.
    [Show full text]
  • Seperation of Subcellular Organelles by Differential and Density Gradient
    SEPARATION OF SUBCELLULAR ORGANELLES BY DIFFIERENTIAL AND DENSITY GWIENT CENTRIFUGATION* DARREL E. GOLL, RONALD B . YOUNG, AND MARVIN H . STROMER Iowa State University INTRODUCTION The centrifuge is now firmly established as an indispensable tool in virtually all phases of modern biology. Centrifugation may be used either: (1)to separate a mixture of different substances in suspension or solution and to isolate these substances into suspensions or solutions containing only one kind of particle; or (2) to characterize the size, shape and density of the particles after they have been separated into purified solutions or suspensions . Consequently, centrifugation can be used in both purification and characterization of a wide range of biological substances. This universality of application mabs the centrifuge invaluable and very widely used in cellular and molecular biology. Because of its practically universal application, an exorbitantly lengthy discussion would be required to review all biological uses of the centrifuge. Consequently, this account will be limited to a discussion of only the first of the two general applications of the centrifuge; namely, use of the centrifuge to separate mixtures of different particles into suspensions or solutions of purified particles. The second general application of the centrifuge, use of centrifugation to characterize size and shape of particles, will not be discussed explicitly in this review. Whenever possible, separations involving muscle tissue or subcellular organelles from muscle tissue will be used to illustrate the usef'ulness of the centrifuge in muscle biology and meat science. The mixtures of different substances that are commonly fractionated by centrifugation may contain either whole intact cells or subcellular components of cells released by rupture of the outer cell membrane.
    [Show full text]