Final Report June 2012 Improving the Status of Lago Amatitlan by Producing Bioenergy

Total Page:16

File Type:pdf, Size:1020Kb

Final Report June 2012 Improving the Status of Lago Amatitlan by Producing Bioenergy Pöyry Energy Oy Tekniikantie 4 A, P.O.Box 93, FI-02151 Espoo, Finland, www.poyry.com Contact Pöyry: Martti Surakka Mob: +358 50 412 0119 Fax: +358 10 33 24338 Email: [email protected] Contact EASA: Peter Hörmander Mob Gua: +502-4247 1307 Mob Int: +46-70 331 16 25 Email: [email protected] Contact Universidad Galileo: Judith Diaz Cabrera Mob : +502- 5403 7852 Tel : +502-2423 8000 ext 4215 Email : [email protected] Date: June 21, 2012 Final Report June 2012 Improving the status of Lago Amatitlan by producing bioenergy. Feasibility study and action plan. 1 Table of Contents 1 EXECUTIVE SUMMARY ................................................................................................... 5 1.1 Findings Galileo .................................................................................................................... 5 1.2 Findings Energía y Ambiente, S.A. and Pöyry OY ............................................................... 5 1.3 Feasibility analysis of a large scale project for utilization of water hyacinths as water treatment and energy source ........................................................................................................... 7 2 INTRODUCTION AND BACKGROUND INFORMATION ........................................... 11 2.1 Introduction ......................................................................................................................... 11 2.2 Presentation of the Consultants and Institutions ................................................................. 13 2.2.1 Pöyry Oy ........................................................................................................................ 13 2.2.2 Energia y Ambiente, S.A. ............................................................................................... 13 2.2.3 Universidad Galileo........................................................................................................ 13 2.2.4 AMSA, Autoridad para el Manejo Sustentable de la Cuenca y del Lago de Amatitlán 14 2.2.5 Feasibility study key personnel ...................................................................................... 14 2.2.6 Responsibilities of parties involved ............................................................................... 15 3 BENEFICIARIES ............................................................................................................... 16 4 OBJECTIVES...................................................................................................................... 16 5 RESULTS OF THE FEASIBILITY STUDY ..................................................................... 17 5.1 Result 1: Classification and characterization of macrophytes and phytoplankton currently present in Lago Amatitlán ............................................................................................................. 19 5.1.1 Task 4: Inventory of macrophytes and algae in Lago Amatitlán (Galileo) .................... 20 5.1.2 Task 5: Characterization of the water chemistry in the Lake (Galileo) ......................... 69 5.2 Result 2: Identification of suitable harvesting techniques for macrophytes and phytoplankton ............................................................................................................................... 75 5.2.1 Task 11: Study of macrophyte and algae harvesting methods (EASA/Pöyry) .............. 75 5.3 Result 3: Identification of suitable biodigesters for macrophytes and phytoplankton ........ 76 5.3.1 Task 9: Study of feasible biodigester technologies (EASA/Pöyry) ............................... 76 5.3.2 Task 14: Biogas production test on different macrophytes and algae strains (EASA/Pöyry) ............................................................................................................................... 81 5.4 Result 4: Pilot biodigester ................................................................................................... 81 5.4.1 Task 10: Study of feasible biogas driven electricity generators (EASA/Pöyry) ............ 81 5.4.2 Task 14: Biogas production test on different macrophyte and algae strains (EASA/Pöyry) ............................................................................................................................... 84 See chapter 5.3.2. .......................................................................................................................... 84 5.4.3 Task 17: Construction of pilot biodigester (EASA/Pöyry) ............................................ 84 5.4.4 Task 20: Commissioning and start-up of biogas pilot plant (EASA/Pöyry) .................. 86 5.5 Result 5: Identification and characterization of lipid producing algae ................................ 87 5.5.1 Task 6: Classification of algae (Galileo) ........................................................................ 87 5.5.2 Task 7: Identification/characterization of lipid producing algae (Galileo) .................... 88 5.6 Result 6: Identification of feasible extraction techniques for lipids .................................... 95 5.7 Result 7: Biodiesel production in laboratory scale .............................................................. 95 2 5.8 Result 8: Conceptual design of water pre-treatment plant using macrophytes in constructed wetlands around the inlet of Río Villalobos in the Northern part of the lake. .............................. 96 5.9 Result 9: Conceptual design of algae cultivation plant ..................................................... 102 5.10 Result 10: Definition of socio-economic impact of the implemented project ............. 105 5.11 Result 11: Definition of environmental impact of the implemented project ................ 106 5.12 Result 12: Definition of procedure for obtaining carbon credits from renewable energy plants such as macrophytes and algae (EASA/Pöyry) ................................................................ 107 5.12.1 Introduction to carbon credits and eligibility of projects ............................................. 107 5.12.2 The CDM project cycle to obtain carbon credits ......................................................... 109 5.12.3 Estimated carbon credits for the Amatitlan bioenergy project ..................................... 111 6 RENEWABLE ENERGY GENERATION PLANT......................................................... 113 6.1 Cultivation area for water hyacinths.................................................................................. 113 6.2 Harvesting area for water hyacinths .................................................................................. 114 6.3 Storage area for harvested and shredded water hyacinths ................................................. 114 6.4 Biodigester ......................................................................................................................... 114 6.5 Treatment area for biogas .................................................................................................. 115 6.6 Storage of purified biogas ................................................................................................. 115 6.7 Storage of digestate and biosludge .................................................................................... 116 6.8 Electrical generator and grid interface .............................................................................. 116 7 MARKET STUDY ............................................................................................................ 117 7.1 Environmental clean-up potential of water hyacinths ....................................................... 117 7.2 Biogas production from water hyacinths ........................................................................... 119 7.3 Digistate for potential fertilizer use ................................................................................... 119 7.4 Sludge for use as compost ................................................................................................. 120 7.5 Carbon credits .................................................................................................................... 120 7.6 Summary of estimated market values................................................................................ 120 8 FEASIBILITY ANALYSIS .............................................................................................. 121 8.1 General and basic assumptions .......................................................................................... 121 8.2 Investment costs ................................................................................................................ 121 8.3 Raw material costs ............................................................................................................. 122 8.4 Operation and maintenance costs ...................................................................................... 122 8.5 Sales income ...................................................................................................................... 123 8.6 Estimation of the feasibility .............................................................................................. 123 9 BUSINESS PLAN ............................................................................................................. 125 9.1 Project strategy .................................................................................................................. 125 9.2 Staffing and capabilities ...................................................................................................
Recommended publications
  • Impact of Procedural Steps and Cryopreservation Agents in the Cryopreservation of Chlorophyte Microalgae
    Impact of Procedural Steps and Cryopreservation Agents in the Cryopreservation of Chlorophyte Microalgae Tony V. L. Bui, Ian L. Ross, Gisela Jakob, Ben Hankamer* Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia Abstract The maintenance of traditional microalgae collections based on liquid and solid media is labour intensive, costly and subject to contamination and genetic drift. Cryopreservation is therefore the method of choice for the maintenance of microalgae culture collections, but success is limited for many species. Although the mechanisms underlying cryopreservation are understood in general, many technical variations are present in the literature and the impact of these are not always elaborated. This study describes two-step cryopreservation processes in which 3 microalgae strains representing different cell sizes were subjected to various experimental approaches to cryopreservation, the aim being to investigate mechanistic factors affecting cell viability. Sucrose and dimethyl sulfoxide (DMSO) were used as cryoprotectants. They were found to have a synergistic effect in the recovery of cryopreserved samples of many algal strains, with 6.5% being the optimum DMSO concentration. The effect of sucrose was shown to be due to improved cell survival and recovery after thawing by comparing the effect of sucrose on cell viability before or after cryopreservation. Additional factors with a beneficial effect on recovery were the elimination of centrifugation steps (minimizing cell damage), the reduction of cell concentration (which is proposed to reduce the generation of toxic cell wall components) and the use of low light levels during the recovery phase (proposed to reduce photooxidative damage). The use of the best conditions for each of these variables yielded an improved protocol which allowed the recovery and subsequent improved culture viability of a further 16 randomly chosen microalgae strains.
    [Show full text]
  • 2021 Tese Rmfranca.Pdf
    UNIVERSIDADE FEDERAL DO CEARÁ CENTRO DE TECNOLOGIA DEPARTAMENTO DE ENGENHARIA HIDRÁULICA E AMBIENTAL PROGRAMA DE PÓS-GRADUAÇÃO EM ENGENHARIA CIVIL RAIMUNDA MOREIRA DA FRANCA ESTRUTURA FITOPLANCTÔNICA EM RESERVATÓRIOS CEARENSES - ASSOCIAÇÃO HIDROCLIMÁTICA E ESTADO TRÓFICO FORTALEZA-CE 2021 RAIMUNDA MOREIRA DA FRANCA ESTRUTURA FITOPLANCTÔNICA EM RESERVATÓRIOS CEARENSES – ASSOCIAÇÃO HIDROCLIMÁTICA E ESTADO TRÓFICO Tese de doutorado apresentada ao Programa de Pós-Graduação em Engenharia Civil da Universidade Federal do Ceará como parte dos requisitos à obtenção do título de doutora em Engenharia Civil (Recursos Hídricos). Área de concentração: Saneamento Ambiental. Orientador: Profº. Dr. Fernando José Araújo da Silva. FORTALEZA-CE 2021 RAIMUNDA MOREIRA DA FRANCA ESTRUTURA FITOPLANCTÔNICA EM RESERVATÓRIOS CEARENSES - ASSOCIAÇÃO HIDROCLIMÁTICA E ESTADO TRÓFICO Tese de doutorado apresentada ao Programa de Pós-Graduação em Engenharia Civil da Universidade Federal do Ceará como parte dos requisitos à obtenção do título de doutora em Engenharia Civil (Recursos Hídricos). Área de concentração: Saneamento Ambiental. Aprovada em: 04/05/2021 BANCA EXAMINADORA ______________________________________________________ Prof. Dr. Fernando José Araújo da Silva (Orientador) Universidade Federal do Ceará (UFC) ________________________________________________________ Prof.ª. Dra. Marisete Dantas de Aquino (Examinadora Interna) Universidade Federal do Ceará (UFC) ________________________________________________________ Prof. Dr. Iran Eduardo Lima Neto (Examinador
    [Show full text]
  • Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016
    Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016 April 1981 Revised, May 1982 2nd revision, April 1983 3rd revision, December 1999 4th revision, May 2011 Prepared for U.S. Department of Commerce Ohio Department of Natural Resources National Oceanic and Atmospheric Administration Division of Wildlife Office of Ocean and Coastal Resource Management 2045 Morse Road, Bldg. G Estuarine Reserves Division Columbus, Ohio 1305 East West Highway 43229-6693 Silver Spring, MD 20910 This management plan has been developed in accordance with NOAA regulations, including all provisions for public involvement. It is consistent with the congressional intent of Section 315 of the Coastal Zone Management Act of 1972, as amended, and the provisions of the Ohio Coastal Management Program. OWC NERR Management Plan, 2011 - 2016 Acknowledgements This management plan was prepared by the staff and Advisory Council of the Old Woman Creek National Estuarine Research Reserve (OWC NERR), in collaboration with the Ohio Department of Natural Resources-Division of Wildlife. Participants in the planning process included: Manager, Frank Lopez; Research Coordinator, Dr. David Klarer; Coastal Training Program Coordinator, Heather Elmer; Education Coordinator, Ann Keefe; Education Specialist Phoebe Van Zoest; and Office Assistant, Gloria Pasterak. Other Reserve staff including Dick Boyer and Marje Bernhardt contributed their expertise to numerous planning meetings. The Reserve is grateful for the input and recommendations provided by members of the Old Woman Creek NERR Advisory Council. The Reserve is appreciative of the review, guidance, and council of Division of Wildlife Executive Administrator Dave Scott and the mapping expertise of Keith Lott and the late Steve Barry.
    [Show full text]
  • An Integrative Approach Sheds New Light Onto the Systematics
    www.nature.com/scientificreports OPEN An integrative approach sheds new light onto the systematics and ecology of the widespread ciliate genus Coleps (Ciliophora, Prostomatea) Thomas Pröschold1*, Daniel Rieser1, Tatyana Darienko2, Laura Nachbaur1, Barbara Kammerlander1, Kuimei Qian1,3, Gianna Pitsch4, Estelle Patricia Bruni4,5, Zhishuai Qu6, Dominik Forster6, Cecilia Rad‑Menendez7, Thomas Posch4, Thorsten Stoeck6 & Bettina Sonntag1 Species of the genus Coleps are one of the most common planktonic ciliates in lake ecosystems. The study aimed to identify the phenotypic plasticity and genetic variability of diferent Coleps isolates from various water bodies and from culture collections. We used an integrative approach to study the strains by (i) cultivation in a suitable culture medium, (ii) screening of the morphological variability including the presence/absence of algal endosymbionts of living cells by light microscopy, (iii) sequencing of the SSU and ITS rDNA including secondary structures, (iv) assessment of their seasonal and spatial occurrence in two lakes over a one‑year cycle both from morphospecies counts and high‑ throughput sequencing (HTS), and, (v) proof of the co‑occurrence of Coleps and their endosymbiotic algae from HTS‑based network analyses in the two lakes. The Coleps strains showed a high phenotypic plasticity and low genetic variability. The algal endosymbiont in all studied strains was Micractinium conductrix and the mutualistic relationship turned out as facultative. Coleps is common in both lakes over the whole year in diferent depths and HTS has revealed that only one genotype respectively one species, C. viridis, was present in both lakes despite the diferent lifestyles (mixotrophic with green algal endosymbionts or heterotrophic without algae).
    [Show full text]
  • Effects of Temperature, Light Intensity and Quality, Carbon Dioxide, and Culture Medium Nutrients on Growth and Lipid Production of Ettlia Oleoabundans
    Effects of Temperature, Light Intensity and Quality, Carbon Dioxide, and Culture Medium Nutrients on Growth and Lipid Production of Ettlia oleoabundans by Ying Yang A Dissertation Submitted to the Faculty of WORCESTER POLYTECHNIC INSTITUTE in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biology and Biotechnology by December 2013 Approved by: Dr. Pamela Weathers, Advisor Dr. Robert Thompson, Committee Member Dr. Luis Vidali, Committee Member Dr. Reeta Rao, Committee Member “A journey of a thousand miles begins with a single step.” — Lao Tzu (604 BC – 531 BC) ii Abstract Ettlia oleoabundans, a freshwater green microalga, was grown under different environmental conditions to study its growth, lipid yield and quality for a better understanding of the fundamental physiology of this oleaginous species. E. oleoabundans showed steady increase in biomass under low temperature and low light intensity, and at high temperature lipid cell content significantly increased independent of nitrate depletion. Studies on light quality showed that red light treatment did not change the biomass concentration, but stimulated lipid yield especially oleic acid, the most desirable biodiesel precursor. Moreover, no photoreversibility in lipid production was observed when applying alternating short-term red and far-red lights, which left the phytochrome effect still an open question. In addition, carbon dioxide enrichment via an air sparging system significantly boosted exponential growth and increased carbon conversion efficiency. Finally, a practical study demonstrated the feasibility of growing E. oleoabundans for high lipid production using a diluted agricultural anaerobic waste effluent as the medium. Together, these studies showed the potential of E. oleoabundans as a promising high yield feedstock for the production of high quality biodiesel.
    [Show full text]
  • Combining and Comparing Coalescent, Distance and Character-Based Approaches for Barcoding Microalgaes: a Test with Chlorella-Like Species (Chlorophyta)
    RESEARCH ARTICLE Combining and Comparing Coalescent, Distance and Character-Based Approaches for Barcoding Microalgaes: A Test with Chlorella-Like Species (Chlorophyta) Shanmei Zou, Cong Fei, Jiameng Song, Yachao Bao, Meilin He, Changhai Wang* Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, PR China a11111 * [email protected] Abstract Several different barcoding methods of distinguishing species have been advanced, but which method is the best is still controversial. Chlorella is becoming particularly promising in the OPEN ACCESS development of second-generation biofuels. However, the taxonomy of Chlorella–like organ- Citation: Zou S, Fei C, Song J, Bao Y, He M, Wang isms is easily confused. Here we report a comprehensive barcoding analysis of Chlorella-like C (2016) Combining and Comparing Coalescent, Distance and Character-Based Approaches for species from Chlorella, Chloroidium, Dictyosphaerium and Actinastrum based on rbcL,ITS, Barcoding Microalgaes: A Test with Chlorella-Like tufA and 16S sequences to test the efficiency of traditional barcoding, GMYC, ABGD, PTP, P Species (Chlorophyta). PLoS ONE 11(4): e0153833. ID and character-based barcoding methods. First of all, the barcoding results gave new doi:10.1371/journal.pone.0153833 insights into the taxonomic assessment of Chlorella-like organisms studied, including the clear Editor: Peter Prentis, Queensland University of species discrimination and resolution of potentially cryptic species complexes in C. sorokini- Technology, AUSTRALIA ana, D. ehrenbergianum and C. Vulgaris.ThetufA proved to be the most efficient barcoding Received: November 17, 2015 locus, which thus could be as potential “specific barcode” for Chlorella-like species.
    [Show full text]
  • An Unrecognized Ancient Lineage of Green Plants Persists in Deep Marine Waters1
    J. Phycol. 46, 1288–1295 (2010) Ó 2010 Phycological Society of America DOI: 10.1111/j.1529-8817.2010.00900.x AN UNRECOGNIZED ANCIENT LINEAGE OF GREEN PLANTS PERSISTS IN DEEP MARINE WATERS1 Frederick W. Zechman2,3 Department of Biology, California State University Fresno, 2555 East San Ramon Ave, Fresno, California 93740, USA Heroen Verbruggen,3 Frederik Leliaert Phycology Research Group, Ghent University, Krijgslaan 281 S8, 9000 Ghent, Belgium Matt Ashworth University Station MS A6700, 311 Biological Laboratories, University of Texas at Austin, Austin, Texas 78712, USA Mark A. Buchheim Department of Biological Science, University of Tulsa, Tulsa, Oklahoma 74104, USA Marvin W. Fawley School of Mathematical and Natural Sciences, University of Arkansas at Monticello, Monticello, Arkansas 71656, USA Department of Biological Sciences, North Dakota State University, Fargo, North Dakota 58105, USA Heather Spalding Botany Department, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA Curt M. Pueschel Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13901, USA Julie A. Buchheim, Bindhu Verghese Department of Biological Science, University of Tulsa, Tulsa, Oklahoma 74104, USA and M. Dennis Hanisak Harbor Branch Oceanographic Institution, Fort Pierce, Florida 34946, USA We provide molecular phylogenetic evidence that Key index words: Chlorophyta; green algae; molec- the obscure genera Palmophyllum Ku¨tz. and Verdigel- ular phylogenetics; Palmophyllaceae fam. nov.; las D. L. Ballant. et J. N. Norris form a distinct and Palmophyllales ord. nov.; Palmophyllum; Prasino- early diverging lineage of green algae. These pal- phyceae; Verdigellas; Viridiplantae melloid seaweeds generally persist in deep waters, Abbreviations: AU, approximately unbiased; BI, where grazing pressure and competition for space Bayesian inference; ML, maximum likelihood; are reduced.
    [Show full text]
  • Catálogo De Las Algas Y Cianoprocariotas Dulciacuícolas De Cuba
    CATÁLOGO DE LAS ALGAS Y CIANOPROCARIOTAS DULCIACUÍCOLAS DE CUBA. EDITORIAL Augusto Comas González UNIVERSO o S U R CATÁLOGO DE LAS ALGAS Y CIANOPROCARIOTAS DULCIACUÍCOLAS DE CUBA. 1 2 CATÁLOGO DE LAS ALGAS Y CIANOPROCARIOTAS DULCIACUÍCOLAS DE CUBA. Augusto Comas González 3 Dirección Editorial: MSc. Alberto Valdés Guada Diseño: D.I. Roberto C. Berroa Cabrera Autor: Augusto Comas González Compilación y edición científica: Augusto Comas González © Reservados todos los derechos por lo que no se permite la reproduc- ción total o parcial de este libro. Editorial UNIVERSO SUR Universidad de Cienfuegos Carretera a Rodas, Km. 4. Cuatro Caminos Cienfuegos, CUBA © ISBN: 978-959-257-228-7 4 Indice INTRODUCCIÓN 7 CYANOPROKARYOTA 9 Clase Cyanophyceae 9 Orden Chroococcales Wettstein 1923 9 Orden Oscillatoriales Elenkin 1934 15 Orden Nostocales (Borzi) Geitler 1925 19 Orden Stigonematales Geitler 1925 22 Clase Chrysophyceae 23 Orden Chromulinales 23 Orden Ochromonadales 23 Orden Prymnesiales 24 Clase Xanthophyceae (= Tribophyceae) 24 Orden Mischococcales Pascher 1913 24 Orden Tribonematales Pascher 1939 25 Orden Botrydiales 26 Orden Vaucheriales 26 Clase Dinophyceae 26 Orden Peridiniales 26 Clase Cryptophyceae 27 Orden Cryptomonadales 27 Clase Rhodophyceae Ruprecht 1851 28 Orden Porphyridiales Kylin 1937 28 Orden Compsopogonales Skuja 1939 28 Orden Nemalionales Schmitz 1892 28 Orden Hildenbrandiales Pueschel & Cole 1982) 29 Orden Ceramiales 29 Clase Glaucocystophyceae Kies et Kremer 1989 29 Clase Euglenophyceae 29 Orden Euglenales 29 Clase Bacillariophyceae 34 Orden Centrales 34 Orden Pennales 35 Clase Prasinophyceae Chadefaud 1950 50 Orden Polyblepharidales Korš. 1938 50 Orden Tetraselmidales Ettl 1983 51 Clase Chlamydophyceae Ettl 1981 51 Orden Chlamydomonadales Frtisch in G.S. West 1927 51 5 Orden Volvocales Oltmanns 1904 52 Orden Chlorococcales Marchand 1895 Orth.
    [Show full text]
  • Genetic Diversity of Symbiotic Green Algae of Paramecium Bursaria Syngens Originating from Distant Geographical Locations
    plants Article Genetic Diversity of Symbiotic Green Algae of Paramecium bursaria Syngens Originating from Distant Geographical Locations Magdalena Greczek-Stachura 1, Patrycja Zagata Le´snicka 1, Sebastian Tarcz 2 , Maria Rautian 3 and Katarzyna Mozd˙ ze˙ ´n 1,* 1 Institute of Biology, Pedagogical University of Krakow, Podchor ˛azych˙ 2, 30-084 Kraków, Poland; [email protected] (M.G.-S.); [email protected] (P.Z.L.) 2 Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016 Krakow, Poland; [email protected] 3 Laboratory of Protistology and Experimental Zoology, Faculty of Biology and Soil Science, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 Saint Petersburg, Russia; [email protected] * Correspondence: [email protected] Abstract: Paramecium bursaria (Ehrenberg 1831) is a ciliate species living in a symbiotic relationship with green algae. The aim of the study was to identify green algal symbionts of P. bursaria originating from distant geographical locations and to answer the question of whether the occurrence of en- dosymbiont taxa was correlated with a specific ciliate syngen (sexually separated sibling group). In a comparative analysis, we investigated 43 P. bursaria symbiont strains based on molecular features. Three DNA fragments were sequenced: two from the nuclear genomes—a fragment of the ITS1-5.8S rDNA-ITS2 region and a fragment of the gene encoding large subunit ribosomal RNA (28S rDNA), Citation: Greczek-Stachura, M.; as well as a fragment of the plastid genome comprising the 30rpl36-50infA genes. The analysis of two Le´snicka,P.Z.; Tarcz, S.; Rautian, M.; Mozd˙ ze´n,K.˙ Genetic Diversity of ribosomal sequences showed the presence of 29 haplotypes (haplotype diversity Hd = 0.98736 for Symbiotic Green Algae of Paramecium ITS1-5.8S rDNA-ITS2 and Hd = 0.908 for 28S rDNA) in the former two regions, and 36 haplotypes 0 0 bursaria Syngens Originating from in the 3 rpl36-5 infA gene fragment (Hd = 0.984).
    [Show full text]
  • Neoproterozoic Origin and Multiple Transitions to Macroscopic Growth in Green Seaweeds
    bioRxiv preprint doi: https://doi.org/10.1101/668475; this version posted June 12, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Neoproterozoic origin and multiple transitions to macroscopic growth in green seaweeds Andrea Del Cortonaa,b,c,d,1, Christopher J. Jacksone, François Bucchinib,c, Michiel Van Belb,c, Sofie D’hondta, Pavel Škaloudf, Charles F. Delwicheg, Andrew H. Knollh, John A. Raveni,j,k, Heroen Verbruggene, Klaas Vandepoeleb,c,d,1,2, Olivier De Clercka,1,2 Frederik Leliaerta,l,1,2 aDepartment of Biology, Phycology Research Group, Ghent University, Krijgslaan 281, 9000 Ghent, Belgium bDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Zwijnaarde, Belgium cVIB Center for Plant Systems Biology, Technologiepark 71, 9052 Zwijnaarde, Belgium dBioinformatics Institute Ghent, Ghent University, Technologiepark 71, 9052 Zwijnaarde, Belgium eSchool of Biosciences, University of Melbourne, Melbourne, Victoria, Australia fDepartment of Botany, Faculty of Science, Charles University, Benátská 2, CZ-12800 Prague 2, Czech Republic gDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA hDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, 02138, USA. iDivision of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee, DD2 5DA, UK jSchool of Biological Sciences, University of Western Australia (M048), 35 Stirling Highway, WA 6009, Australia kClimate Change Cluster, University of Technology, Ultimo, NSW 2006, Australia lMeise Botanic Garden, Nieuwelaan 38, 1860 Meise, Belgium 1To whom correspondence may be addressed. Email [email protected], [email protected], [email protected] or [email protected].
    [Show full text]
  • Phylogenetic Position of Ecballocystis and Ecballocystopsis (Chlorophyta)
    Fottea, Olomouc, 13(1): 65–75, 2013 65 Phylogenetic position of Ecballocystis and Ecballocystopsis (Chlorophyta) Shuang XIA1, 2, Huan ZHU1, 2 Ying–Yin CHENG1, Guo–Xiang LIU1* & Zheng–Yu HU1 1Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China; *Corresponding author e–mail: [email protected] 2University of Chinese Academy of Sciences, Beijing 100039, P. R. China Abstract: Ecballocystis and Ecballocystopsis are two rare, green algal genera. Both have thalli that grow on rock surfaces in flowing water and attach to rocks by a thick mucilaginous pad. The algae have similar thalli structure, consisting of autospores in mother cell wall remnants. Ecballocystopsis differs from Ecballocystis in being filamentous instead of dendroid. In this study, new strains of Ecballocystis hubeiensis and Ecballocystopsis dichotomus were collected from China and cultured. Morphology was observed by light and electron microscopy. 18S rDNA and rbcL sequences were determined and subjected to phylogenetic analysis. Both morphological and phylogenetic analysis indicated that Ecballocystis and Ecballocystopsis should be placed in the family of Oocystaceae. Ecballocystis was closely related to Elongatocystis in 18S rDNA phylogeny. The results of present study emphasize the high level of phenotypic plasticity of Oocystaceae. Within the family, cell arrangement can be solitary, colonial, dendroid, or filamentous. Key words: Ecballocystis, Ecballocystopsis, phylogeny, 18S rDNA, rbcL, Oocystaceae Introduction the species from other members of Ecballocystis (LIU & HU 2005). Ecballocystis BOHLIN and Ecballocystopsis The genus Ecballocystopsis was esta- IYENGAR are two rare green algal genera. Both blished by IYENGAR (1933) with the type species algae grow on the wet surfaces of substrata.
    [Show full text]
  • DIMITAR VALEV: Wastewater Treatment with Algae Doctoral Dissertation, 118 Pp
    ANNALES UNIVERSITATIS TURKUENSIS UNIVERSITATIS ANNALES AI 627 AI Dimitar Valev WASTEWATER TREATMENT WITH ALGAE Dimitar Valev Painosalama Oy, Turku, Finland 2020 Finland Turku, Oy, Painosalama ISBN 978-951-29-8094-9 (PRINT) – ISBN 978-951-29-8095-6 (PDF) TURUN YLIOPISTON JULKAISUJA ANNALES UNIVERSITATIS TURKUENSIS ISSN 0082-7002 (Print) SARJA – SER. AI OSA – TOM. 627 | ASTRONOMICA – CHEMICA – PHYSICA – MATHEMATICA | TURKU 2020 ISSN 2343-3175 (Online) WASTEWATER TREATMENT WITH ALGAE Dimitar Valev TURUN YLIOPISTON JULKAISUJA – ANNALES UNIVERSITATIS TURKUENSIS SARJA – SER. AI OSA – TOM. 627 | ASTRONOMICA – CHEMICA – PHYSICA – MATHEMATICA | TURKU 2020 University of Turku Faculty of Science and Engineering Department of Biochemistry / Molecular Plant Biology Doctoral programme in Molecular Life Sciences Supervised by Dr. Esa Tyystjärvi Dr. Taina Tyystjärvi Department of Biochemistry / Department of Biochemistry / Molecular Plant Biology, Molecular Plant Biology, University of Turku, FI-20014 University of Turku, FI-20014 Turku, Finland Turku, Finland Dr. Taras Antal Department of Botany and Plant Ecology Pskov State University Pskov 180000 Russia Reviewed by Professor Amit Bhatnagar Professor Koenraad Muylaert Water Chemistry & Microbiology Laboratory of Aquatic Biology University of Eastern Finland KU Leuven Kuopio, Finland Kortrijk, Belgium Opponent Professor Ondřej Prášil Centre Algatech Institute of Microbiology, The Czech Academy of Sciences Třeboň, Czech Republic The originality of this publication has been checked in accordance with the University of Turku quality assurance system using the Turnitin OriginalityCheck service. ISBN 978-951-29-8094-9 (PRINT) ISBN 978-951-29-8095-6 (PDF) ISSN 0082-7002 (Painettu/Print) ISSN 2343-3175 (Sähköinen/Online) Painosalama Oy, Turku, Finland 2020 UNIVERSITY OF TURKU Faculty of Science and Engineering Department of Biochemistry Molecular Plant Biology DIMITAR VALEV: Wastewater treatment with algae Doctoral Dissertation, 118 pp.
    [Show full text]