DOCSLIB.ORG

Palmophyllum Crassum , a New Record of an Ancient Species In

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Palmophyllum Crassum , a New Record of an Ancient Species In ISSN 1226-9999 (print) ISSN 2287-7851 (online) Korean J. Environ. Biol. 35(3) : 319~328 (2017) https://doi.org/10.11626/KJEB.2017.35.3.319 <Original article> Palmophyllum crassum, a New Record of an Ancient Species in Green Algae from Korea Hyung Woo Lee and Myung Sook Kim* Department of Biology, Jeju National University, Jeju 63243, Republic of Korea Abstract - The continuous exploration in deep seawater from Korea makes us lead the discovery of ancient Chlorophyta, Palmophyllum, in the Korean coast. The phylogenetic analyses of 18S rRNA and rbcL genes demonstrate that our specimens are Palmophyllum crassum (Naccari) Rabenhorst, recorded in Japan and clearly distinguished from P. umbracola from New Zealand and California, USA. Palmophyllum crassum grows in the subtidal region, 8-30 m deep, and has a crustose thallus which is closely adherent to substrates such as non-geniculate crustose coralline algae, sponge, shells, or rocks. P. crassum is composed of numerous spherical cells embedded in the gelatinous matrix. The discovery of this ancient green seaweed implies that the Korean coast is one of the hotspots of algal species diversity and has the suitable marine environment for algal speciation. We suggest the grounds to conserve the Korean coast environmentally as the biodiversity center of marine species by studying the phylogeny of seaweeds. Key words : 18S rRNA, molecular phylogeny, Palmophyllophyceae, Palmophyllum crassum, rbcL INTRODUCTION that the earliest-diverging Chlorophyta comprises marine green algae with simple morphology by revealing a deep- The green algae distributed in freshwater and seawater, branching clade which is a macroscopic algal group named even terrestrial habitats, are photosynthetic eukaryotes char- as the order Palmophyllales including Palmophyllum, Ver- acterized by the presence of chloroplast with two envelope digellas and Palmoclathrus, based on the molecular phylo- membranes, stacked thylakoids, and chlorophyll a and b genetic study. with different morphological organizations such as mona- The palmelloid green algae, Palmophyllum, Palmoclathrus doid, palmelloid, coccoid, coenocytic, and filamentous and Verdigellas, thrive in deep seawater up to >100 m deep, (Pröschold and Leliaert 2007). This green lineage has been where has dimly light marine habitat (Womersley 1971; an essential member in the global ecosystem for a long time Nelson and Ryan 1986; Ballantine and Aponte 1996). These as the ancestor of land plants evolved by the early diver- seaweeds are characterized by a unique multicellularity, gence of Streptophyta and Chlorophyta, from an ancestral forming firm and well-defined macroscopic thalli, referred green flagellate unicell (Leliaert et al. 2012). Therefore, it to as palmelloid thallus organization (Zechman et al. 2010). was generally recognized that the ancestral Chlorophyta is Despite the lack of evidence of the systematic position unicellular with the presence of flagella and organic body within the green algae, these genera had been classified into scales, but this concept has been a matter of debate (Leliaert the family Palmellaceae of the chlorophycean order Volvo- et al. 2016). Recently, Zechman et al. (2010) challenged cales (Fritsch 1935), the family Palmellaceae of the order Tetrasporales (Womersley 1984), or the family Chlorophy- * ‌Corresponding author: Myung Sook Kim, Tel. 064-754-3523, ceae of the order Chlorococcales (Nelson and Ryan 1984). Fax. 064-756-3541, E-mail. [email protected] Recently, since the molecular phylogenetic assessment of ⓒ2017. Korean Society of Environmental Biology. 320 Hyung Woo Lee and Myung Sook Kim Table 1. ‌Collection information and GenBank accession number of Palmophyllum crassum (Naccari) Rubenhorst specimens analyzed in this study GenBank accession No. Taxa Collection information Habit (depth/substratum) Voucher 18S rRNA rbcL Gwideok 1-ri, Jeju, Korea; 25-30 m deep / reef with MSK-GA00830 - MF769365 31 May 2011 crustose coralline algae Chujado, Jeju, Korea; 13-15 m deep / reef with MSK-GA00915 MF769362 MF769367 1 Oct. 2011 crustose coralline algae Udo, Jeju, Korea; 18-25 m deep / reef with MSK-GA00912 - MF769366 1 Nov. 2011 crustose coralline algae Udo, Jeju, Korea; 18-25 m deep / reef with MSK131101-10 MF769361 MF769368 Palmophyllum crassum 1 Nov. 2011 crustose coralline algae (Naccari) Rubenhorst Udo, Jeju, Korea; 18-25 m deep / reef with MSK131101-11 - MF769369 1 Nov. 2011 crustose coralline algae Hyeongjeseom, Busan, Korea; 10-15 m deep / reef with MSK160728-02 MF769363 - 28 Jul. 2016 sponge Shimoda, Shizuoka, Japan; 8-15 m deep / reef with MSK170417-01 MF769364 - 17 Apr. 2017 crustose coralline algae Shimoda, Shizuoka, Japan; 8-15 m deep / reef with MSK170417-03 - MF769370 17 Apr. 2017 sponge the plastid-encoded atpB and rbcL genes and the nuclear- analyses. In addition, to compare with the entities from encoded 18S rRNA gene resolved that these palmelloid Korea, we carried out a collection in Japan. The aims of this genera are positioned to the early diverged lineage in Chlo- study are to identify new green algal entities collected from rophyta forming monophyly in order level, the order Palmo- deep subtidal and to resolve the species diversity of Korean phyllales and the family Palmophyllaceae were established green seaweed by reconstructing the green algal phylogeny (Zeckman et al. 2010). Further, this early evolutionary lin- with recognition of ancient lineage. eage was elevated to the class Palmophyllophyceae, based on the chloroplast phylogenomic analyses (Leliaert et al. 2016). MATERIALS AND METHODS The genus Palmophyllum is reported only two species, P. crassum (Naccari) Rabenhorst and P. umbracola Nelson et The samples were collected using SCUBA (from depths Ryan, all over the world (Guiry and Guiry 2017). P. crassum of 8-30 m) in the southern part of Korea including Jeju has been known as the cosmopolitan species, unlike P. Island and the central coast of Japan (Table 1). Underwater umbracola, which is mainly restricted in the Southern images were obtained using a digital camera (Stylus TG-4, hemisphere (Nelson and Ryan 1984). Although it has been Olympus, Japan). Samples were transported to the labora- recorded in Japan (Yoshida et al. 1990), several investiga- tory and photographed by Canon Powershot G7X camera tions of Korean algal flora have not recognized P. crassum (Canon, Japan). After describing the external morphology, distributed in the deep region (Kang et al. 2011; Kang and voucher specimens were made into pressed ones on the her- Kim 2012). barium sheets. Samples for DNA analyses were detached Recently, the molecular methods have been applied to from the thallus and dried via silica-gel. Samples for ana- green seaweeds from Korean coasts to conduct taxonomic tomical investigations were preserved in 5.0% formalin in reexamination or to describe species diversity (Lee and Kim seawater. Sections were done by a bench-top freezing micro- 2011, 2015). By continuous exploration of deep subtidal tome (NK-101-II; Nippon Optical Works Co., Ltd., Tokyo, regions from Korean coastlines, we obtained crustose and Japan). Photomicrographs were obtained using a BX43 firmly gelatinous green algal specimens unrecognized pre- microscope (Olympus, Tokyo, Japan), with an EOS 600D viously and undertook the molecular and morphological digital camera (Canon, Japan). The digitized images were Palmophyllum crassum from Korea 321 imported into Adobe Photoshop ver. 6.1 software (Adobe RESULTS Systems Inc., San Jose, CA, USA). Voucher specimens were deposited in the herbarium of Jeju National University We analyzed a set of 41 SSU 18S rRNA gene sequences (JNUB), Korea and the National Institute of Biological Re- including the four Palmophyllum crassum (MF769361- sources (NIBR), Incheon, Korea. MF769364) collected from Korea and Japan (Table 1), the DNA was extracted using the DNeasy Plant Mini Kit representative taxa in charophytes, prasinophytes, and core (Qiagen, Hilden, Germany) following the manufacturer’s chlorophytes with Palmophyllophyceae, Palmophyllum instructions. Following DNA extraction, the polymerase umbracola (FJ619275 and FJ619276) and Verdigella peltata chain reaction (PCR) were conducted to amplify the nuclear- (FJ619277 and LT174528). The SSU 18S rRNA data set encoded small subunit (SSU) 18S rRNA gene and the consisted of 1897 characters. Among all sites, 758 (56.1%) ribulose-1, 5-biphospate carboxylase/oxygenage (rbcL). were variable and 506 (26.7%) were parsimoniously infor- Amplification was performed in a 20-μL reaction, using mative. The phylogenetic tree constructed by ML and BI AccuPower PCR PreMix (Bioneer, Daejeon, Korea) and was congruent, and we exhibited the ML topology including Swift MaxPro thermal cyclers (ESCO, Singapore). The pri- the bootstrap (BS) value in ML and posterior probability (PP) mer and PCR protocols for SSU 18S rRNA were followed in BI (Fig. 1). Ulvophyceae-Trebouxiophyceae-Chlorophy- by Saunders and Moore (2013), and those for rbcL were ceae (UTC) and Streptophyta formed each distinct clade in followed by Heesch et al. (2009). PCR products were pu- the SSU phylogenetic tree, but prasinophytes were polyphy- rified using the AccuPrep Purification Kit (Bioneer) and letic. Our four P. crassum specimens collected from Korea sequenced commercially at Macrogen (Seoul, Korea). and Japan were identical, and they formed an independent Sequence edition and alignment were carried out using clade with Palmophyllum umbracola from USA (FJ619275) Chromas version 1.45 software (Queensland,
Load more

Recommended publications
  • Impact of Procedural Steps and Cryopreservation Agents in the Cryopreservation of Chlorophyte Microalgae
    Impact of Procedural Steps and Cryopreservation Agents in the Cryopreservation of Chlorophyte Microalgae Tony V. L. Bui, Ian L. Ross, Gisela Jakob, Ben Hankamer* Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia Abstract The maintenance of traditional microalgae collections based on liquid and solid media is labour intensive, costly and subject to contamination and genetic drift. Cryopreservation is therefore the method of choice for the maintenance of microalgae culture collections, but success is limited for many species. Although the mechanisms underlying cryopreservation are understood in general, many technical variations are present in the literature and the impact of these are not always elaborated. This study describes two-step cryopreservation processes in which 3 microalgae strains representing different cell sizes were subjected to various experimental approaches to cryopreservation, the aim being to investigate mechanistic factors affecting cell viability. Sucrose and dimethyl sulfoxide (DMSO) were used as cryoprotectants. They were found to have a synergistic effect in the recovery of cryopreserved samples of many algal strains, with 6.5% being the optimum DMSO concentration. The effect of sucrose was shown to be due to improved cell survival and recovery after thawing by comparing the effect of sucrose on cell viability before or after cryopreservation. Additional factors with a beneficial effect on recovery were the elimination of centrifugation steps (minimizing cell damage), the reduction of cell concentration (which is proposed to reduce the generation of toxic cell wall components) and the use of low light levels during the recovery phase (proposed to reduce photooxidative damage). The use of the best conditions for each of these variables yielded an improved protocol which allowed the recovery and subsequent improved culture viability of a further 16 randomly chosen microalgae strains.
    [Show full text]
  • Perspectives in Phycology Vol
    Perspectives in Phycology Vol. 3 (2016), Issue 3, p. 141–154 Article Published online June 2016 Diversity and ecology of green microalgae in marine systems: an overview based on 18S rRNA gene sequences Margot Tragin1, Adriana Lopes dos Santos1, Richard Christen2,3 and Daniel Vaulot1* 1 Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 7144, Station Biologique, Place Georges Teissier, 29680 Roscoff, France 2 CNRS, UMR 7138, Systématique Adaptation Evolution, Parc Valrose, BP71. F06108 Nice cedex 02, France 3 Université de Nice-Sophia Antipolis, UMR 7138, Systématique Adaptation Evolution, Parc Valrose, BP71. F06108 Nice cedex 02, France * Corresponding author: [email protected] With 5 figures in the text and an electronic supplement Abstract: Green algae (Chlorophyta) are an important group of microalgae whose diversity and ecological importance in marine systems has been little studied. In this review, we first present an overview of Chlorophyta taxonomy and detail the most important groups from the marine environment. Then, using public 18S rRNA Chlorophyta sequences from culture and natural samples retrieved from the annotated Protist Ribosomal Reference (PR²) database, we illustrate the distribution of different green algal lineages in the oceans. The largest group of sequences belongs to the class Mamiellophyceae and in particular to the three genera Micromonas, Bathycoccus and Ostreococcus. These sequences originate mostly from coastal regions. Other groups with a large number of sequences include the Trebouxiophyceae, Chlorophyceae, Chlorodendrophyceae and Pyramimonadales. Some groups, such as the undescribed prasinophytes clades VII and IX, are mostly composed of environmental sequences. The 18S rRNA sequence database we assembled and validated should be useful for the analysis of metabarcode datasets acquired using next generation sequencing.
    [Show full text]
  • Acanthophora Dendroides Harvey (Rhodomelaceae), a New Record for the Atlantic and Pacific Oceans
    15 3 NOTES ON GEOGRAPHIC DISTRIBUTION Check List 15 (3): 509–514 https://doi.org/10.15560/15.3.509 Acanthophora dendroides Harvey (Rhodomelaceae), a new record for the Atlantic and Pacific oceans Gabriela C. García-Soto, Juan M. Lopez-Bautista The University of Alabama, Department of Biological Sciences, Science and Engineering Complex, 1325 Hackberry Ln, Tuscaloosa, AL 35401, USA. Corresponding author: Gabriela García-Soto, [email protected] Abstract We record Acanthophora dendroides Harvey for the first time in the Atlantic Ocean. Two specimens from the Philip- pines were resolved as conspecific to the Atlantic A. dendroides in molecular analyses extending its geographic range to the Philippines. In light of new evidence provided by field-collected specimens ofAcanthophora spicifera (M.Vahl) Børgesen (generitype) from Florida and Venezuela, the flattened species A. pacifica(Setchell) Kraft, showed no affin- ity to Acanthophora sensu stricto, suggesting that the genus should be restricted to cylindrical species only. Key words Atlantic Ocean, Philippines, taxonomy. Academic editor: Luciane Fontana da Silva | Received 8 October 2018 | Accepted 21 January 2019 | Published 21 June 2019 Citation: García-Soto GC, Lopez-Bautista JM (2019) Acanthophora dendroides Harvey (Rhodomelaceae), a new record for the Atlantic and Pacific oceans. Check List 15 (3): 509–514. https://doi.org/10.15560/15.3.509 Introduction Kraft are restricted to the Pacific Ocean (Guiry and Guiry 2018), A. dendroides Harvey to the Indian Ocean The genus Acanthophora J.V. Lamouroux 1813 is a (Silva et al. 1996) and A. ramulosa Lindenb. ex. Kutz- member of the tribe Chondrieae and it is distinguished from other genera of the tribe by the presence of spirally ing appears to be confined to the Gulf of Guinea in West arranged acute spines (Gordon-Mills and Womersley Africa (Steentoft 1967).
    [Show full text]
  • Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016
    Old Woman Creek National Estuarine Research Reserve Management Plan 2011-2016 April 1981 Revised, May 1982 2nd revision, April 1983 3rd revision, December 1999 4th revision, May 2011 Prepared for U.S. Department of Commerce Ohio Department of Natural Resources National Oceanic and Atmospheric Administration Division of Wildlife Office of Ocean and Coastal Resource Management 2045 Morse Road, Bldg. G Estuarine Reserves Division Columbus, Ohio 1305 East West Highway 43229-6693 Silver Spring, MD 20910 This management plan has been developed in accordance with NOAA regulations, including all provisions for public involvement. It is consistent with the congressional intent of Section 315 of the Coastal Zone Management Act of 1972, as amended, and the provisions of the Ohio Coastal Management Program. OWC NERR Management Plan, 2011 - 2016 Acknowledgements This management plan was prepared by the staff and Advisory Council of the Old Woman Creek National Estuarine Research Reserve (OWC NERR), in collaboration with the Ohio Department of Natural Resources-Division of Wildlife. Participants in the planning process included: Manager, Frank Lopez; Research Coordinator, Dr. David Klarer; Coastal Training Program Coordinator, Heather Elmer; Education Coordinator, Ann Keefe; Education Specialist Phoebe Van Zoest; and Office Assistant, Gloria Pasterak. Other Reserve staff including Dick Boyer and Marje Bernhardt contributed their expertise to numerous planning meetings. The Reserve is grateful for the input and recommendations provided by members of the Old Woman Creek NERR Advisory Council. The Reserve is appreciative of the review, guidance, and council of Division of Wildlife Executive Administrator Dave Scott and the mapping expertise of Keith Lott and the late Steve Barry.
    [Show full text]
  • Neoproterozoic Origin and Multiple Transitions to Macroscopic Growth in Green Seaweeds
    Neoproterozoic origin and multiple transitions to macroscopic growth in green seaweeds Andrea Del Cortonaa,b,c,d,1, Christopher J. Jacksone, François Bucchinib,c, Michiel Van Belb,c, Sofie D’hondta, f g h i,j,k e Pavel Skaloud , Charles F. Delwiche , Andrew H. Knoll , John A. Raven , Heroen Verbruggen , Klaas Vandepoeleb,c,d,1,2, Olivier De Clercka,1,2, and Frederik Leliaerta,l,1,2 aDepartment of Biology, Phycology Research Group, Ghent University, 9000 Ghent, Belgium; bDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Zwijnaarde, Belgium; cVlaams Instituut voor Biotechnologie Center for Plant Systems Biology, 9052 Zwijnaarde, Belgium; dBioinformatics Institute Ghent, Ghent University, 9052 Zwijnaarde, Belgium; eSchool of Biosciences, University of Melbourne, Melbourne, VIC 3010, Australia; fDepartment of Botany, Faculty of Science, Charles University, CZ-12800 Prague 2, Czech Republic; gDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742; hDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138; iDivision of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, United Kingdom; jSchool of Biological Sciences, University of Western Australia, WA 6009, Australia; kClimate Change Cluster, University of Technology, Ultimo, NSW 2006, Australia; and lMeise Botanic Garden, 1860 Meise, Belgium Edited by Pamela S. Soltis, University of Florida, Gainesville, FL, and approved December 13, 2019 (received for review June 11, 2019) The Neoproterozoic Era records the transition from a largely clear interpretation of how many times and when green seaweeds bacterial to a predominantly eukaryotic phototrophic world, creat- emerged from unicellular ancestors (8). ing the foundation for the complex benthic ecosystems that have There is general consensus that an early split in the evolution sustained Metazoa from the Ediacaran Period onward.
    [Show full text]
  • 1 Integrative Biology 200 "PRINCIPLES OF
    Integrative Biology 200 "PRINCIPLES OF PHYLOGENETICS" Spring 2018 University of California, Berkeley B.D. Mishler March 14, 2018. Classification II: Phylogenetic taxonomy including incorporation of fossils; PhyloCode I. Phylogenetic Taxonomy - the argument for rank-free classification A number of recent calls have been made for the reformation of the Linnaean hierarchy (e.g., De Queiroz & Gauthier, 1992). These authors have emphasized that the existing system is based in a non-evolutionary world-view; the roots of the Linnaean hierarchy are in a specially- created world-view. Perhaps the idea of fixed, comparable ranks made some sense under that view, but under an evolutionary world view they don't make sense. There are several problems with the current nomenclatorial system: 1. The current system, with its single type for a name, cannot be used to precisely name a clade. E.g., you may name a family based on a certain type specimen, and even if you were clear about what node you meant to name in your original publication, the exact phylogenetic application of your name would not be clear subsequently, after new clades are added. 2. There are not nearly enough ranks to name the thousands of levels of monophyletic groups in the tree of life. Therefore people are increasingly using informal rank-free names for higher- level nodes, but without any clear, formal specification of what clade is meant. 3. Most aspects of the current code, including priority, revolve around the ranks, which leads to instability of usage. For example, when a change in relationships is discovered, several names often need to be changed to adjust, including those of groups whose circumscription has not changed.
    [Show full text]
  • An Unrecognized Ancient Lineage of Green Plants Persists in Deep Marine Waters1
    J. Phycol. 46, 1288–1295 (2010) Ó 2010 Phycological Society of America DOI: 10.1111/j.1529-8817.2010.00900.x AN UNRECOGNIZED ANCIENT LINEAGE OF GREEN PLANTS PERSISTS IN DEEP MARINE WATERS1 Frederick W. Zechman2,3 Department of Biology, California State University Fresno, 2555 East San Ramon Ave, Fresno, California 93740, USA Heroen Verbruggen,3 Frederik Leliaert Phycology Research Group, Ghent University, Krijgslaan 281 S8, 9000 Ghent, Belgium Matt Ashworth University Station MS A6700, 311 Biological Laboratories, University of Texas at Austin, Austin, Texas 78712, USA Mark A. Buchheim Department of Biological Science, University of Tulsa, Tulsa, Oklahoma 74104, USA Marvin W. Fawley School of Mathematical and Natural Sciences, University of Arkansas at Monticello, Monticello, Arkansas 71656, USA Department of Biological Sciences, North Dakota State University, Fargo, North Dakota 58105, USA Heather Spalding Botany Department, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA Curt M. Pueschel Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13901, USA Julie A. Buchheim, Bindhu Verghese Department of Biological Science, University of Tulsa, Tulsa, Oklahoma 74104, USA and M. Dennis Hanisak Harbor Branch Oceanographic Institution, Fort Pierce, Florida 34946, USA We provide molecular phylogenetic evidence that Key index words: Chlorophyta; green algae; molec- the obscure genera Palmophyllum Ku¨tz. and Verdigel- ular phylogenetics; Palmophyllaceae fam. nov.; las D. L. Ballant. et J. N. Norris form a distinct and Palmophyllales ord. nov.; Palmophyllum; Prasino- early diverging lineage of green algae. These pal- phyceae; Verdigellas; Viridiplantae melloid seaweeds generally persist in deep waters, Abbreviations: AU, approximately unbiased; BI, where grazing pressure and competition for space Bayesian inference; ML, maximum likelihood; are reduced.
    [Show full text]
  • Catálogo De Las Algas Y Cianoprocariotas Dulciacuícolas De Cuba
    CATÁLOGO DE LAS ALGAS Y CIANOPROCARIOTAS DULCIACUÍCOLAS DE CUBA. EDITORIAL Augusto Comas González UNIVERSO o S U R CATÁLOGO DE LAS ALGAS Y CIANOPROCARIOTAS DULCIACUÍCOLAS DE CUBA. 1 2 CATÁLOGO DE LAS ALGAS Y CIANOPROCARIOTAS DULCIACUÍCOLAS DE CUBA. Augusto Comas González 3 Dirección Editorial: MSc. Alberto Valdés Guada Diseño: D.I. Roberto C. Berroa Cabrera Autor: Augusto Comas González Compilación y edición científica: Augusto Comas González © Reservados todos los derechos por lo que no se permite la reproduc- ción total o parcial de este libro. Editorial UNIVERSO SUR Universidad de Cienfuegos Carretera a Rodas, Km. 4. Cuatro Caminos Cienfuegos, CUBA © ISBN: 978-959-257-228-7 4 Indice INTRODUCCIÓN 7 CYANOPROKARYOTA 9 Clase Cyanophyceae 9 Orden Chroococcales Wettstein 1923 9 Orden Oscillatoriales Elenkin 1934 15 Orden Nostocales (Borzi) Geitler 1925 19 Orden Stigonematales Geitler 1925 22 Clase Chrysophyceae 23 Orden Chromulinales 23 Orden Ochromonadales 23 Orden Prymnesiales 24 Clase Xanthophyceae (= Tribophyceae) 24 Orden Mischococcales Pascher 1913 24 Orden Tribonematales Pascher 1939 25 Orden Botrydiales 26 Orden Vaucheriales 26 Clase Dinophyceae 26 Orden Peridiniales 26 Clase Cryptophyceae 27 Orden Cryptomonadales 27 Clase Rhodophyceae Ruprecht 1851 28 Orden Porphyridiales Kylin 1937 28 Orden Compsopogonales Skuja 1939 28 Orden Nemalionales Schmitz 1892 28 Orden Hildenbrandiales Pueschel & Cole 1982) 29 Orden Ceramiales 29 Clase Glaucocystophyceae Kies et Kremer 1989 29 Clase Euglenophyceae 29 Orden Euglenales 29 Clase Bacillariophyceae 34 Orden Centrales 34 Orden Pennales 35 Clase Prasinophyceae Chadefaud 1950 50 Orden Polyblepharidales Korš. 1938 50 Orden Tetraselmidales Ettl 1983 51 Clase Chlamydophyceae Ettl 1981 51 Orden Chlamydomonadales Frtisch in G.S. West 1927 51 5 Orden Volvocales Oltmanns 1904 52 Orden Chlorococcales Marchand 1895 Orth.
    [Show full text]
  • Lateral Gene Transfer of Anion-Conducting Channelrhodopsins Between Green Algae and Giant Viruses
    bioRxiv preprint doi: https://doi.org/10.1101/2020.04.15.042127; this version posted April 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 5 Lateral gene transfer of anion-conducting channelrhodopsins between green algae and giant viruses Andrey Rozenberg 1,5, Johannes Oppermann 2,5, Jonas Wietek 2,3, Rodrigo Gaston Fernandez Lahore 2, Ruth-Anne Sandaa 4, Gunnar Bratbak 4, Peter Hegemann 2,6, and Oded 10 Béjà 1,6 1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel. 2Institute for Biology, Experimental Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, Berlin 10115, Germany. 3Present address: Department of Neurobiology, Weizmann 15 Institute of Science, Rehovot 7610001, Israel. 4Department of Biological Sciences, University of Bergen, N-5020 Bergen, Norway. 5These authors contributed equally: Andrey Rozenberg, Johannes Oppermann. 6These authors jointly supervised this work: Peter Hegemann, Oded Béjà. e-mail: [email protected] ; [email protected] 20 ABSTRACT Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity 1,2. Four ChR families are currently known. Green algal 3–5 and cryptophyte 6 cation-conducting ChRs (CCRs), cryptophyte anion-conducting ChRs (ACRs) 7, and the MerMAID ChRs 8. Here we 25 report the discovery of a new family of phylogenetically distinct ChRs encoded by marine giant viruses and acquired from their unicellular green algal prasinophyte hosts.
    [Show full text]
  • Ribosomal Dna Phylogeny of the Bangiophycidae (Rhodophyta) and the Origin of Secondary Plastids1
    American Journal of Botany 88(8): 1390±1400. 2001. RIBOSOMAL DNA PHYLOGENY OF THE BANGIOPHYCIDAE (RHODOPHYTA) AND THE ORIGIN OF SECONDARY PLASTIDS1 KIRSTEN M. MUÈ LLER,2,6 MARIANA C. OLIVEIRA,3 ROBERT G. SHEATH,2 AND DEBASHISH BHATTACHARYA4,5 2Department of Botany and Dean's Of®ce, University of Guelph, Guelph, Ontario, Canada, N1G 2W1; 3Department of Botany, Institute of Biosciences, University of SaÄo Paulo, R. MataÄo, Travessa 14, N. 321, SaÄo Paulo, SP, Brazil, CEP 05508-900; and 4Department of Biological Sciences, University of Iowa, 239 Biology Building, Iowa City, Iowa 52242-3110 USA We sequenced the nuclear small subunit ribosomal DNA coding region from 20 members of the Bangiophycidae and from two members of the Florideophycidae to gain insights into red algal evolution. A combined alignment of nuclear and plastid small subunit rDNA and a data set of Rubisco protein sequences were also studied to complement the understanding of bangiophyte phylogeny and to address red algal secondary symbiosis. Our results are consistent with a monophyletic origin of the Florideophycidae, which form a sister-group to the Bangiales. Bangiales monophyly is strongly supported, although Porphyra is polyphyletic within Bangia. Ban- giophycidae orders such as the Porphyridiales are distributed over three independent red algal lineages. The Compsopogonales sensu stricto, consisting of two freshwater families, Compsopogonaceae and Boldiaceae, forms a well-supported monophyletic grouping. The single taxon within the Rhodochaetales, Rhodochaete parvula, is positioned within a cluster containing members of the Erythropelti- dales. Analyses of Rubisco sequences show that the plastids of the heterokonts are most closely related to members of the Cyanidiales and are not directly related to cryptophyte and haptophyte plastid genomes.
    [Show full text]
  • Neoproterozoic Origin and Multiple Transitions to Macroscopic Growth in Green Seaweeds
    bioRxiv preprint doi: https://doi.org/10.1101/668475; this version posted June 12, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Neoproterozoic origin and multiple transitions to macroscopic growth in green seaweeds Andrea Del Cortonaa,b,c,d,1, Christopher J. Jacksone, François Bucchinib,c, Michiel Van Belb,c, Sofie D’hondta, Pavel Škaloudf, Charles F. Delwicheg, Andrew H. Knollh, John A. Raveni,j,k, Heroen Verbruggene, Klaas Vandepoeleb,c,d,1,2, Olivier De Clercka,1,2 Frederik Leliaerta,l,1,2 aDepartment of Biology, Phycology Research Group, Ghent University, Krijgslaan 281, 9000 Ghent, Belgium bDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Zwijnaarde, Belgium cVIB Center for Plant Systems Biology, Technologiepark 71, 9052 Zwijnaarde, Belgium dBioinformatics Institute Ghent, Ghent University, Technologiepark 71, 9052 Zwijnaarde, Belgium eSchool of Biosciences, University of Melbourne, Melbourne, Victoria, Australia fDepartment of Botany, Faculty of Science, Charles University, Benátská 2, CZ-12800 Prague 2, Czech Republic gDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA hDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, 02138, USA. iDivision of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee, DD2 5DA, UK jSchool of Biological Sciences, University of Western Australia (M048), 35 Stirling Highway, WA 6009, Australia kClimate Change Cluster, University of Technology, Ultimo, NSW 2006, Australia lMeise Botanic Garden, Nieuwelaan 38, 1860 Meise, Belgium 1To whom correspondence may be addressed. Email [email protected], [email protected], [email protected] or [email protected].
    [Show full text]
  • Refinements for the Amplification and Sequencing of Red Algal DNA Barcode and Redtol Phylogenetic Markers: a Summary of Current Primers, Profiles and Strategies
    Review Algae 2013, 28(1): 31-43 http://dx.doi.org/10.4490/algae.2013.28.1.031 Open Access Refinements for the amplification and sequencing of red algal DNA barcode and RedToL phylogenetic markers: a summary of current primers, profiles and strategies Gary W. Saunders1,* and Tanya E. Moore1 1Centre for Environmental and Molecular Algal Research, Department of Biology, University of New Brunswick, Frederic- ton, NB E3B 5A3, Canada This review provides a comprehensive summary of the PCR primers and profiles currently in use in our laboratory for red algal DNA barcoding and phylogenetic research. While work focuses on florideophyte taxa, many of the markers have been applied successfully to the Bangiales, as well as other lineages previously assigned to the Bangiophyceae sensu lato. All of the primers currently in use with their respective amplification profiles and strategies are provided, which can include full fragment, overlapping fragments and what might best be called “informed overlapping fragments”, i.e., a fragment for a marker is amplified and sequenced for a taxon and those sequence data are then used to identify the best primers to amplify the remaining fragment(s) for that marker. We extend this strategy for the more variable markers with sequence from the external PCR primers used to “inform” the selection of internal sequencing primers. This summary will hopefully serve as a useful resource to systematists in the red algal community. Key Words: DNA barcode; Florideophyceae; molecular markers; phylogeny; polymerase chain reaction; primers; RedToL INTRODUCTION There can be little question that molecular techniques redtol/) (Vis et al. 2010).
    [Show full text]