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Potential of Chlorella Species As Feedstock for Bioenergy Production: a Review
Environmental and Climate Technologies 2020, vol. 24, no. 2, pp. 203–220 https://doi.org/10.2478/rtuect-2020-0067 https://content.sciendo.com Potential of Chlorella Species as Feedstock for Bioenergy Production: A Review Baiba IEVINA1*, Francesco ROMAGNOLI2 1, 2 Institute of Energy systems and environment, Riga Technical University, Āzenes 12-k1, Riga, Latvia Abstract – Selection of appropriate microalgae strain for cultivation is essential for overall success of large-scale biomass production under particular environmental and climate conditions. In addition to fast growth rate and biomass productivity, the species ability to grow in wastewater must also be considered to increase the economic feasibility of microalgae for bioenergy purposes. Furthermore, the content of bioactive compounds in a strain must be taken into account to further increase the viability by integration of biorefinery concept. Chlorella spp. are among the most studied microalgal species. The present review attempts to unfold the potential of species of the genus Chlorella for bioenergy production integrating applicability for wastewater treatment and production of high added-value compounds. Several key features potentially make Chlorella spp. highly beneficial for bioenergy production. Fast growth rate, low nutritional requirements, low sensitivity to contamination, adaptation to fluctuating environments, ability to grow in photoautotrophic, heterotrophic and mixotrophic conditions make Chlorella spp. highly useful for outdoor cultivation coupled with wastewater treatment. Chlorella is a source of multiple bioactive compounds. Most promising high-value products are chlorophylls, lutein, β-carotene and lipids. Here we demonstrate that although many Chlorella spp. show similar characteristics, some substantial differences in growth and response to environmental factors exist. -
Super Green Superfoods
[Plant-Based Ingredients] Vol. 17 No. 12 December 2012 Super Green Superfoods By Celeste Sepessy, Associate Editor Once the secret of Birkenstock-wearing progressives, green foods are now filling the shopping carts of informed—if not guilty—conventional consumers. Nutrient-dense greens date back millions of years, and humans in the know have been eating them for centuries; green foods manufacturers commonly tout their ingredients as the energy food of ancient Mesoamericans, namely the Aztecs. The term "green foods" encompasses a range of raw materials including algae (chlorella, spirulina, etc.), grasses (alfalfa, barley grass, wheat grass, etc.) and common green vegetables (broccoli, spinach, etc.). Though each ingredient boasts its own benefits, they all pack a well-rounded nutritional punch not often found elsewhere. "Spirulina is nature's multivitamin," said John Blanco, president of AnMar International, noting the microalgae has 60-percent protein, unsaturated fatty acids and vitamin precursors, such as amino acids and proenzymes. "It's not a complete 100-percent balanced vitamin tablet, but it's pretty close." And this nutritional breakdown is similar across the green board, as the ingredients are densely filled with phytonutrients, antioxidants, vitamins, minerals and nucleic acid, among other nutrients. Consumers of all demographics are becoming more aware of the benefits of eating these green superfoods; Guinevere Lynn, director of business development at Sun Chlorella, pointed to the media for the industry's popularity surge. "Mass media has certainly played a major role in this 'green renaissance,' " she explained, citing Dr. Oz's help in particular. The television medical personality is a huge proponent of green foods, and Dr. -
Chlorophyta, Trebouxiophyceae) in Lake Tanganyika (Africa)*
Biologia 63/6: 799—805, 2008 Section Botany DOI: 10.2478/s11756-008-0101-4 Siderocelis irregularis (Chlorophyta, Trebouxiophyceae) in Lake Tanganyika (Africa)* Maya P. Stoyneva1, Elisabeth Ingolič2,WernerKofler3 &WimVyverman4 1Sofia University ‘St Kliment Ohridski’, Faculty of Biology, Department of Botany, 8 bld. Dragan Zankov, BG-1164 Sofia, Bulgaria; e-mail: [email protected], [email protected]fia.bg 2Graz University of Technology, Research Institute for Electron Microscopy, Steyrergasse 17,A-8010 Graz, Austria; e-mail: [email protected] 3University of Innsbruck, Institute of Botany, Sternwartestrasse 15,A-6020 Innsbruck, Austria; e-mail: werner.kofl[email protected] 4Ghent University, Department Biology, Laboratory of Protistology and Aquatic Ecology, Krijgslaan 281-S8,B-9000 Gent, Belgium; e-mail: [email protected] Abstract: Siderocelis irregularis Hindák, representing a genus Siderocelis (Naumann) Fott that is known from European temperate waters, was identified as a common phytoplankter in Lake Tanganyika. It was found aposymbiotic as well as ingested (possibly endosymbiotic) in lake heterotrophs, mainly Strombidium sp. and Vorticella spp. The morphology and ultrastructure of the species, studied with LM, SEM and TEM, are described with emphasis on the structure of the cell wall and the pyrenoid. Key words: Chlorophyta; cell wall; pyrenoid; symbiosis; ciliates; Strombidium; Vorticella Introduction ics of symbiotic species in general came into alignment with that of free-living algae and the term ‘zoochlorel- Tight partnerships between algae and aquatic inver- lae’ was abandoned as being taxonomically ambiguous tebrates, including symbiotic relationships, have long (e.g. Bal 1968; Reisser & Wiessner 1984; Taylor 1984; been of interest and a number of excellent reviews are Reisser 1992a). -
A Novel Treatment Protects Chlorella at Commercial Scale from the Predatory Bacterium Vampirovibrio Chlorellavorus
fmicb-07-00848 June 17, 2016 Time: 15:28 # 1 ORIGINAL RESEARCH published: 20 June 2016 doi: 10.3389/fmicb.2016.00848 A Novel Treatment Protects Chlorella at Commercial Scale from the Predatory Bacterium Vampirovibrio chlorellavorus Eneko Ganuza1*, Charles E. Sellers1, Braden W. Bennett2, Eric M. Lyons1 and Laura T. Carney2 1 Microbiology Group, Heliae Development, LLC, Gilbert, AZ, USA, 2 Molecular Ecology Group, Heliae Development, LLC, Gilbert, AZ, USA The predatory bacterium, Vampirovibrio chlorellavorus, can destroy a Chlorella culture in just a few days, rendering an otherwise robust algal crop into a discolored suspension of empty cell walls. Chlorella is used as a benchmark for open pond cultivation due to its fast growth. In nature, V. chlorellavorus plays an ecological role by controlling this widespread terrestrial and freshwater microalga, but it can have a devastating effect when it attacks large commercial ponds. We discovered that V. chlorellavorus was associated with the collapse of four pilot commercial-scale (130,000 L volume) open- Edited by: pond reactors. Routine microscopy revealed the distinctive pattern of V. chlorellavorus Yoav Bashan, The Bashan Institute of Science, USA attachment to the algal cells, followed by algal cell clumping, culture discoloration and Reviewed by: ultimately, growth decline. The “crash” of the algal culture coincided with increasing Kathleen Scott, proportions of 16s rRNA sequencing reads assigned to V. chlorellavorus. We designed University of South Florida, USA a qPCR assay to predict an impending culture crash and developed a novel treatment to Qiang Wang, Chinese Academy of Sciences, China control the bacterium. We found that (1) Chlorella growth was not affected by a 15 min *Correspondence: exposure to pH 3.5 in the presence of 0.5 g/L acetate, when titrated with hydrochloric Eneko Ganuza acid and (2) this treatment had a bactericidal effect on the culture (2-log decrease [email protected] in aerobic counts). -
Effects of Chlorella Vulgaris As a Feed Ingredient on the Quality and Nutritional Value of Weaned Piglets’ Meat
foods Article Effects of Chlorella vulgaris as a Feed Ingredient on the Quality and Nutritional Value of Weaned Piglets’ Meat Cátia F. Martins 1,2 , José M. Pestana 1, Cristina M. Alfaia 1 ,Mónica Costa 1 , David M. Ribeiro 2 , Diogo Coelho 1 , Paula A. Lopes 1 , André M. Almeida 2, João P. B. Freire 2 and José A. M. Prates 1,* 1 CIISA—Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de Lisboa, 1300-477 Lisbon, Portugal; [email protected] (C.F.M.); [email protected] (J.M.P.); [email protected] (C.M.A.); [email protected] (M.C.); [email protected] (D.C.); [email protected] (P.A.L.) 2 LEAF—Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisbon, Portugal; [email protected] (D.M.R.); [email protected] (A.M.A.); [email protected] (J.P.B.F.) * Correspondence: [email protected] Abstract: Chlorella vulgaris (CH) is usually considered a feed supplement in pig nutrition, and its use as an ingredient is poorly studied. Among many interesting characteristics, this microalga has high protein levels and can be a putative alternative for soybean meal. Our aim was to study the effect of a 5% CH incorporation in the diet, individually or combined with two carbohydrases, on meat quality traits and nutritional value. Forty-four post-weaned male piglets individually housed, with an initial live weight of 11.2 ± 0.46 kg, were randomly distributed into four experimental groups: Citation: Martins, C.F.; Pestana, J.M.; control (n = 11, without CH) and three groups fed with 5% CH incorporation, plain (n = 10), with Alfaia, C.M.; Costa, M.; Ribeiro, D.M.; 0.005% Rovabio® Excel AP (n = 10), and with 0.01% of a pre-selected four-CAZyme mixture (n = 11). -
An Integrative Approach Sheds New Light Onto the Systematics
www.nature.com/scientificreports OPEN An integrative approach sheds new light onto the systematics and ecology of the widespread ciliate genus Coleps (Ciliophora, Prostomatea) Thomas Pröschold1*, Daniel Rieser1, Tatyana Darienko2, Laura Nachbaur1, Barbara Kammerlander1, Kuimei Qian1,3, Gianna Pitsch4, Estelle Patricia Bruni4,5, Zhishuai Qu6, Dominik Forster6, Cecilia Rad‑Menendez7, Thomas Posch4, Thorsten Stoeck6 & Bettina Sonntag1 Species of the genus Coleps are one of the most common planktonic ciliates in lake ecosystems. The study aimed to identify the phenotypic plasticity and genetic variability of diferent Coleps isolates from various water bodies and from culture collections. We used an integrative approach to study the strains by (i) cultivation in a suitable culture medium, (ii) screening of the morphological variability including the presence/absence of algal endosymbionts of living cells by light microscopy, (iii) sequencing of the SSU and ITS rDNA including secondary structures, (iv) assessment of their seasonal and spatial occurrence in two lakes over a one‑year cycle both from morphospecies counts and high‑ throughput sequencing (HTS), and, (v) proof of the co‑occurrence of Coleps and their endosymbiotic algae from HTS‑based network analyses in the two lakes. The Coleps strains showed a high phenotypic plasticity and low genetic variability. The algal endosymbiont in all studied strains was Micractinium conductrix and the mutualistic relationship turned out as facultative. Coleps is common in both lakes over the whole year in diferent depths and HTS has revealed that only one genotype respectively one species, C. viridis, was present in both lakes despite the diferent lifestyles (mixotrophic with green algal endosymbionts or heterotrophic without algae). -
Light and Growth Medium Effect on Chlorella Vulgaris Biomass Production
Journal of Environmental Chemical Engineering 2 (2014) 665–674 Contents lists available at ScienceDirect Journal of Environmental Chemical Engineering journal homepage: www.elsevier.com/locate/jece Light and growth medium effect on Chlorella vulgaris biomass production Matthew Forrest Blair, Bahareh Kokabian, Veera Gnaneswar Gude * Civil and Environmental Engineering Department, Mississippi State University, Mississippi State, MS 39762, USA ARTICLE INFO ABSTRACT Article history: Algae can serve as feedstock for many high value bioproducts and biofuels production. The key to Received 27 June 2013 economic algal biomass production is to optimize the growth conditions. This study presents the effect of Received in revised form 6 November 2013 light wavelengths and growth medium composition on the growth of Chlorella vulgaris. Different light Accepted 6 November 2013 wavelengths [blue, clear (white), green, and red] were used to test their effect on algal growth. Growth media formulations were varied to optimize the growth media composition for maximized algal biomass Keywords: production. Experimental study was conducted in three phases to evaluate: (1) the effect of different Nutrient optimization light wavelengths; (2) the effect of the recommended growth medium at 25%, 50%, and 100% of Growth rates suggested composition; and (3) the effect of nutrient concentrations (nitrogen and phosphorous). The Chlorella vulgaris Light effect effect of these factors was evaluated through specific algal growth rates and volumetric biomass Volumetric biomass productivity productivities during the entire growth period. In this study, blue light performed better (higher growth rate and biomass productivity) at longer growth periods (10–14 days) compared to clear, red and green light wavelengths. The growth media and nutrient effect results indicate that the growth of C. -
As Source of Fermentable Sugars Via Cell Wall Enzymatic Hydrolysis
SAGE-Hindawi Access to Research Enzyme Research Volume 2011, Article ID 405603, 5 pages doi:10.4061/2011/405603 Research Article Evaluation of Chlorella (Chlorophyta) as Source of Fermentable Sugars via Cell Wall Enzymatic Hydrolysis Marcoaurelio´ Almenara Rodrigues1 and Elba Pinto da Silva Bon2 1 Laboratory of Studies Applicable to Photosynthesis (LEAF), Biochemistry Department, Center of Technology, Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Bloco A, Avenida Athos da Silveira Ramos 149, Ilha do Fundao,˜ 21 941-909 Rio de Janeiro, RJ, Brazil 2 Enzyme Technology Laboratory (ENZITEC), Biochemistry Department, Center of Technology, Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Bloco A, Avenida Athos da Silveira Ramos 149, Ilha do Fundao,˜ 21 941-909 Rio de Janeiro, RJ, Brazil Correspondence should be addressed to Marcoaurelio´ Almenara Rodrigues, [email protected] Received 23 December 2010; Accepted 28 February 2011 Academic Editor: Mohamed Kheireddine Aroua Copyright © 2011 M. A. Rodrigues and E. P. D. S. Bon. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ThecellwallofChlorella is composed of up to 80% carbohydrates including cellulose. In this study, Chlorella homosphaera and Chlorella zofingiensis were evaluated as source of fermentable sugars via their cell wall enzymatic degradation. The algae were cultivated in inorganic medium, collected at the stationary growth phase and centrifuged. The cell pellet was suspended in citrate buffer, pH 4.8 and subjected to 24 hours hydrolysis at 50◦C using a cellulases, xylanases, and amylases blend. -
Biosorption Potential of the Microchlorophyte Chlorella Vulgaris for Some
lizers & rti P e e F s f Hussein et al., J Fertil Pestic 2017, 8:1 t o i c l i a d e n DOI: 10.4172/2471-2728.1000177 r s u o J Journal of Fertilizers & Pesticides ISSN: 2471-2728 Research Article Open Access Biosorption Potential of the Microchlorophyte Chlorella vulgaris for Some Pesticides Mervat H Hussein1, Ali M Abdullah2*, Noha I Badr El Din1 and El Sayed I Mishaqa2 1Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt 2Reference Laboratory for Drinking Water, Holding Company for Water and Wastewater, Cairo, Egypt Abstract Nowadays, pollution of either surface or ground water with pesticides is considered as one of the greatest challenges facing Humanity and being a national consideration in Egypt. Agricultural activities are the point source of pesticides that polluting water bodies. The present study investigated the potentiality of Chlorella vulgaris for bioremoval of pesticides mixture of 0.1 mg/mL for each component (Atrazine, Molinate, Simazine, Isoproturon, Propanil, Carbofuran, Dimethoate, Pendimethalin, Metoalcholar, Pyriproxin) either as free cells or immobilized in alginate. Two main experiments were conducted including short- term study having 60 min contact time using fresh free and lyophilized cells and other long-term study having five days incubation period using free and immobilized cells. In the short-term study, the presence of living cells led to bioremoval percentage ranged from 86 to 89 and the lyophilized algal biomass achieved bioremoval ranged from 96% to 99%. In long-term study, the presence of growing algae resulted in pesticides bioremoval ranged from 87% to 96.5%. -
Genetic Engineering of Green Microalgae for the Production of Biofuel and High Value Products
GENETIC ENGINEERING OF GREEN MICROALGAE FOR THE PRODUCTION OF BIOFUEL AND HIGH VALUE PRODUCTS Joanna Beata Szaub Department of Structural and Molecular Biology University College London A thesis submitted for the degree of Doctor of Philosophy August 2012 DECLARATION I, Joanna Beata Szaub confirm that the work presented in this thesis is my own. Where information has been derived from other sources, I confirm that this has been indicated in the thesis. Signed: 1 ABSTRACT A major consideration in the exploitation of microalgae as biotechnology platforms is choosing robust, fast-growing strains that are amenable to genetic manipulation. The freshwater green alga Chlorella sorokiniana has been reported as one of the fastest growing and thermotolerant species, and studies in this thesis have confirmed strain UTEX1230 as the most productive strain of C. sorokiniana with doubling time under optimal growth conditions of less than three hours. Furthermore, the strain showed robust growth at elevated temperatures and salinities. In order to enhance the productivity of this strain, mutants with reduced biochemical and functional PSII antenna size were isolated. TAM4 was confirmed to have a truncated antenna and able to achieve higher cell density than WT, particularly in cultures under decreased irradiation. The possibility of genetic engineering this strain has been explored by developing molecular tools for both chloroplast and nuclear transformation. For chloroplast transformation, various regions of the organelle’s genome have been cloned and sequenced, and used in the construction of transformation vectors. However, no stable chloroplast transformant lines were obtained following microparticle bombardment. For nuclear transformation, cycloheximide-resistant mutants have been isolated and shown to possess specific missense mutations within the RPL41 gene. -
Non-Axenic Microalgae Cultivation in Space – Challenges for the Membrane Μgpbr of the ISS Experiment PBR@LSR
48th International Conference on Environmental Systems ICES-2018-186 8-12 July 2018, Albuquerque, New Mexico Non-axenic microalgae cultivation in space – Challenges for the membrane µgPBR of the ISS experiment PBR@LSR Harald Helisch1, Stefan Belz2, Jochen Keppler3, Gisela Detrell4, Norbert Henn5, Stefanos Fasoulas6, Reinhold Ewald7 Institute of Space Systems, University of Stuttgart, Germany and Oliver Angerer8 German Aerospace Center (DLR), Bonn, Germany Keywords: PBR@LSR, ISS experiment, microalgae, Chlorella vulgaris, long-term cultivation, biofilm The spaceflight experiment PBR@LSR (Photobioreactor at the Life Support Rack) shall demonstrate for the first time the technology and performance of a hybrid life support system – a combination of physico-chemical and biotechnological components – under real space conditions during an operation period of 180 days. To be launched to the International Space Station (ISS) in 2018, PBR@LSR combines the carbon dioxide (CO2) concentrator of ESA’s Life Support Rack (LSR) with an advanced microalgae photobioreactor (PBR). Accommodated in the Destiny module, LSR will concentrate CO2 from the cabin atmosphere. A dedicated interface allows the utilization of the highly concentrated surplus CO2 for the cultivation of the green microalgae species Chlorella vulgaris. Current research at the University of Stuttgart focuses on the fundamental investigation and optimization of non-axenic cultivation processes in µg capable membrane PBRs. This includes the characterization of influences of accompanying bacteria on the non-axenic microalgae culture stability within the PBR suspension loop, photosynthetic capacity as well as overall biomass composition. This paper discusses in general possible influences of emerging bacteria- or algae induced biofilm formation and cell clustering due to non-axenic processing on the long term functionality of µg adapted PBR systems, e.g. -
A First Insight Into the Genome of Prototheca Wickerhamii, a Major
Bakuła et al. BMC Genomics (2021) 22:168 https://doi.org/10.1186/s12864-021-07491-8 RESEARCH ARTICLE Open Access A first insight into the genome of Prototheca wickerhamii, a major causative agent of human protothecosis Zofia Bakuła1, Paweł Siedlecki2,3, Robert Gromadka4, Jan Gawor4, Agnieszka Gromadka3, Jan J. Pomorski5, Hanna Panagiotopoulou5 and Tomasz Jagielski1* Abstract Background: Colourless microalgae of the Prototheca genus are the only known plants that have consistently been implicated in a range of clinically relevant opportunistic infections in both animals and humans. The Prototheca algae are emerging pathogens, whose incidence has increased importantly over the past two decades. Prototheca wickerhamii is a major human pathogen, responsible for at least 115 cases worldwide. Although the algae are receiving more attention nowadays, there is still a substantial knowledge gap regarding their biology, and pathogenicity in particular. Here we report, for the first time, the complete nuclear genome, organelle genomes, and transcriptome of the P. wickerhamii type strain ATCC 16529. Results: The assembled genome size was of 16.7 Mbp, making it the smallest and most compact genome sequenced so far among the protothecans. Key features of the genome included a high overall GC content (64.5%), a high number (6081) and proportion (45.9%) of protein-coding genes, and a low repetitive sequence content (2.2%). The vast majority (90.6%) of the predicted genes were confirmed with the corresponding transcripts upon RNA-sequencing analysis. Most (93.2%) of the genes had their putative function assigned when searched against the InterProScan database. A fourth (23.3%) of the genes were annotated with an enzymatic activity possibly associated with the adaptation to the human host environment.