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Final Report National Potato Breeding Program

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Final Report National Potato Breeding Program Final Report National Potato Breeding Program: Strategic Trait Development Tony Slater The Dept of Economic Development & Resources Project Number: PT08033 PT08033 This project has been funded by Horticulture Innovation Australia Limited using funds from the Australian Government and the Potato industry levy Horticulture Innovation Australia Limited (Hort Innovation) makes no representations and expressly disclaims all warranties (to the extent permitted by law) about the accuracy, completeness, or currency of information in National Potato Breeding Program: Strategic Trait Development. Reliance on any information provided by Hort Innovation is entirely at your own risk. Hort Innovation is not responsible for, and will not be liable for, any loss, damage, claim, expense, cost (including legal costs) or other liability arising in any way (including from Hort Innovation or any other person’s negligence or otherwise) from your use or non-use of National Potato Breeding Program: Strategic Trait Development, or from reliance on information contained in the material or that Hort Innovation provides to you by any other means. ISBN 0 7341 3684 6 Published and distributed by: Horticulture Innovation Australia Limited Level 8, 1 Chifley Square Sydney NSW 2000 Tel: (02) 8295 2300 Fax: (02) 8295 2399 © Copyright 2015 Final Report for Horticulture Australia Project No: PT08033 (31st July 2012) Project Title: National potato breeding program: Strategic trait development Project Leader: Tony Slater Department of Primary Industries, Toolangi Private Bag 15, Ferntree Gully DC VIC 3156 Phone: 03 9210 9342 Email: [email protected] Key Personnel: John Forster, Noel Cogan, Lee Schultz, Maria Lombardi, Ben Hayes, Brendan Rodoni, Mirko Milinkovic, Linda Zheng, Graeme Wilson and Mark Verstraten Funding sources: This Project has been funded by HAL using the fresh and processing potato industry levy and matched funds from the Federal Government, and the Victorian Department of Primary Industries. Published by the Department of Primary Industries, July 2012. © State of Victoria 2012. This publication is the copyright of the Department of Primary Industries and Horticulture Australia Limited. No part may be reproduced by any process except in accordance with the provisions of the Copyright Act 1968. Authorised by the Department of Primary Industries, 1 Spring Street, Melbourne 3000. Disclaimer: This publication may be of assistance to you but the State of Victoria and its employees do not guarantee that the publication is without flaw of any kind or is wholly appropriate for your particular purposes and therefore disclaims all liability for any error, loss or other consequence which may arise from you relying on any information in this publication. Any recommendations contained in this publication do not necessarily represent current HAL Limited policy. No person should act on the basis of the content of this publication, whether as to matters of fact or opinion or other content, without first obtaining specific, independent professional advice in respect of the matters set out in this publication. Date: July 2012. Contents CONTENTS ...................................................................................................................................................................... 1 MEDIA SUMMARY ........................................................................................................................................................ 3 TECHNICAL SUMMARY .............................................................................................................................................. 4 INTRODUCTION ............................................................................................................................................................ 6 OBJECTIVES ..................................................................................................................................................................... 7 MATERIALS AND METHODS ..................................................................................................................................... 8 1. REVIEW OF LITERATURE AND THE PUBLICLY AVAILABLE RESOURCES. ....................................................................... 8 2. INTERNATIONAL LINKAGES. ........................................................................................................................................ 8 3. POTATO GENOTYPE IDENTITY KIT .............................................................................................................................. 8 4. PRIORITIES FOR MAS .................................................................................................................................................. 9 5. POTATO CYST NEMATODE RESISTANCE MARKER DEVELOPMENT ................................................................................ 9 Plant Material ........................................................................................................................................................ 10 PCN phenotyping assay ...................................................................................................................................... 10 PCN marker assays ............................................................................................................................................. 11 6. POTATO VIRUS Y AND POTATO VIRUS X RESISTANCE MARKER DEVELOPMENT ............................................................ 13 PVY sequencing ................................................................................................................................................... 14 PVY and PVX phenotyping assays .................................................................................................................... 17 PVY marker assays .............................................................................................................................................. 19 PVX marker assays .............................................................................................................................................. 20 7. TOMATO SPOTTED WILT VIRUS ................................................................................................................................... 20 TSWV phenotyping assay .................................................................................................................................... 21 8. VERTICILLIUM WILT .................................................................................................................................................. 22 STM1051 marker assay ...................................................................................................................................... 22 9. MULTIPLEXED MARKER ASSAYS ............................................................................................................................... 23 10. COST OF CONVENTIONAL SCREENING COMPARED TO MAS. .................................................................................... 23 11. PROGENY TESTING AND THE DEVELOPMENT OF ESTIMATED BREEDING VALUES ..................................................... 23 Population development and trials ..................................................................................................................... 24 Phenotyping methods .......................................................................................................................................... 25 Data analysis ......................................................................................................................................................... 26 RESULTS ........................................................................................................................................................................ 28 1. REVIEW OF THE LITERATURE AND PUBLICLY AVAILABLE RESOURCES ...................................................................... 28 Potato genetics ..................................................................................................................................................... 28 The potential for marker-assisted selection in potato ..................................................................................... 29 Publicly Available Resources .............................................................................................................................. 31 Potato marker-assisted selection ....................................................................................................................... 32 2. INTERNATIONAL LINKAGES ....................................................................................................................................... 34 3. POTATO GENOTYPE IDENTITY KIT ............................................................................................................................ 35 4. PRIORITISING TRAITS FOR MAS ................................................................................................................................ 37 5. PCN MARKER EVALUATION ...................................................................................................................................... 38 6. PVY AND PVX MARKER EVALUATION ..................................................................................................................... 40 PVY sequencing ..................................................................................................................................................
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