J Genet Resour 2019;5(2): 118-129 Homepage: http://sc.journals.umz.ac.ir/ DOI: 10.22080/jgr.2019.2408 RESEARCH ARTICLE Phylogenetic Assessment of Some Species of Crocus Genus Using DNA Barcoding Fatemeh Aghighiravan, Majid Shokrpour*, Vahideh Nazeri and Mohammad-Reza Naghavi Department of Horticulture, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran A R T I C L E I N F O A B S T R A C T Article history: DNA barcoding is a simple method for the identification of any species using a Received 14 April 2019 short genetic sequence from a standard genome section. The present study Accepted 25 June 2019 aimed at examining the nuclear and chloroplast diversity as well as the Available online 12 July 2019 phylogenetic relationships of eight species of saffron including four spring- flowering and five autumn-flowering species from different parts of Iran, using Keywords: the nuclear barcode and chloroplast genes to specify their evolutionary Saffron pathway and to examine their genetic affinity. First, PCR was performed using Wild species the primers designed based on the chloroplast barcodes of matK, trnL, and PCR rbcL, and nuclear barcode of ITS. Then, the PCR products were purified and Cluster analysis DNA marker sequenced. The results of the phylogenetic tree indicated that the examined saffron species of Iran were separated from each other based on the sequences *Corresponding author: of ITS, matK, and trnL barcodes. In the rbcL barcode, the relationships of a M, Shokrpour number of species remained unresolved, and this genetic region could not
[email protected] appropriately discriminate the species.