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CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya
CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya RENATO GOBBI VETTORAZZI UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO - UENF CAMPOS DOS GOYTACAZES - RJ FEVEREIRO - 2020 CRIOPRESERVAÇÃO DE ORQUÍDEAS BRASILEIRAS DO GÊNERO Cattleya RENATO GOBBI VETTORAZZI “Tese apresentada ao Centro de Ciências e Tecnologias Agropecuárias da Universidade Estadual do Norte Fluminense Darcy Ribeiro, como parte das exigências para obtenção do título de Doutor em Genética e Melhoramento de Plantas” Orientadora: Prof.ª Virginia Silva Carvalho CAMPOS DOS GOYTACAZES - RJ FEVEREIRO – 2020 FICHA CATALOGRÁFICA UENF - Bibliotecas Elaborada com os dados fornecidos pelo autor. V592 Vettorazzi, Renato Gobbi. Criopreservação de orquídeas brasileiras do gênero Cattleya / Renato Gobbi Vettorazzi. - Campos dos Goytacazes, RJ, 2020. 118 f. : il. Bibliografia: 80 - 93. Tese (Doutorado em Genética e Melhoramento de Plantas) - Universidade Estadual do Norte Fluminense Darcy Ribeiro, Centro de Ciências e Tecnologias Agropecuárias, 2020. Orientadora: Virginia Silva Carvalho. 1. Orchidaceae. 2. Banco Criogênico. 3. Vitrificação. 4. Manutenção da Diversidade. 5. Conservação de Germoplasma. I. Universidade Estadual do Norte Fluminense Darcy Ribeiro. II. Título. CDD - 631.5233 AGRADECIMENTOS A Deus, pela proteção, graças e bênçãos concedidas; À minha esposa Milena, por não medir esforços em ajudar, pelo companheirismo, carinho, paciência, compreensão e, sobretudo, pelo amor sempre presente; À minha família, pelas orações e por me fazer uma pessoa digna. Em especial, -
Autumn Plants of the Peloponnese
Autumn Plants of the Peloponnese Naturetrek Tour Report 24 - 31 October 2018 Crocus goulimyi Chelmos Mystras Galanthus reginae-olgae Report& images by David Tattersfield Naturetrek Mingledown Barn Wolf's Lane Chawton Alton Hampshire GU34 3HJ UK T: +44 (0)1962 733051 E: [email protected] W: www.naturetrek.co.uk Tour Report Autumn Plants of the Peloponnese Tour participants: David Tattersfield (leader) and seven clients Day 1 Wednesday 24th October We made rapid progress along the motorway and stopped at Corinth to view the canal, which effectively makes the Peloponnese an island. Here we found our first flowers, the extremely common Autumn Squill Prospero autumnale, the striped, hooded spathes of Friar’s Cowl Arisarum vulgare, and a number of Crocus mazziaricus. A few butterflies included Long-tailed Blue, Lang’s Short-tailed Blue, Eastern Bath White, Mallow Skipper and a Pigmy Skipper. We continued along the newly-completed coast road, before turning inland and climbing steeply into the mountains. We arrived in Kalavrita around 6pm and after settling in to our hotel, we enjoyed a delicious meal of home-cooked food at a nearby taverna. Day 2 Thursday 25th October We awoke to a sunny day with cloud over the mountains. Above Kalavrita, we explored an area of Kermes Oak scrub and open pasture, where we found more white Crocus mazziaricus and Crocus melantherus. Crocus melantherus, as its name suggests can be distinguished from other autumn-flowering species by its black anthers and purple feathering on the outer tepals. Cyclamen hederifolium was common under the shade of the trees. -
New World Orchids Dr
NWO Plant List March 2015 New World Orchids Dr. Kristen Uthus 9588 Meadowcrest, Manchester, MI 48158 Phone: 734-369-1776 Email: [email protected] www.newworldorchids.com Price list 12 March 2015 **Prices are subject to change at any time** PLEASE CHECK OUR WEBSITE FOR INFO ON CULTURE AND AVAILABILITY BEFORE PLACING AN ORDER ! Neofinetia - PRICED PER GROWTH Code Variety Price Description NF001AKA Akabana 赤花 $20.00 Nice clear pink flower. NF002AKE Akebono 曙 $55.00 Green leaves with a lime-green to gray-silver center stripe. NF003AKO Akogare 憧 $80.00 Nice bean leave type and yellow variegation on leaves. NF008AMA Amanogawa 天の川 $15.00 White basal color; 8" LS. NF009AFU Aojiko Fugaku 靑軸富嶽 $40.00 Very special yellow - varying striping. Rare. NF010ACH Aojiku Chousentetsu 青軸朝鮮鉄 $10.00 Pine needle foliage; 3” LS. NF011AON Aone Zuiun 青根瑞雲 $30.00 Similar to Zuiun; very nice tiger color NF012ASA Asahiden 朝日殿 $30.00 Rising Sun Lord, Yellow and green striped leaves. 6"-7" LS; particularly strong fragrance. NF013AWA Awa Haribeni 阿波針紅 $10.00 Pine-needle plant with some pink on the flowers. NF014AYA Ayanishiki 綾錦 $20.00 Large pink flowers and a pink peduncle. NF016BKA Benikanzashi 紅簪 $85.00 3-spur pink flower (see also Hanakanzashi 花簪) NF017BMU Benimuso 紅無双 $45.00 Small, thick peacock, rough textured leaf. 1 NWO Plant List March 2015 Neofinetia - PRICED PER GROWTH Code Variety Price Description NF018BTO Beniotome 紅乙女 $60.00 Pink flower, pleasant wave to leaves. NF019BOU Beniougi (Benikujaku Kohai) 紅扇 $100.00 Red blush on leaves, red tsuke. NF020BSU Benisuzume 紅雀 $20.00 Princess leaf plants with smaller, solid light pink flowers. -
Phylogenetic Assessment of Some Species of Crocus Genus Using DNA Barcoding
J Genet Resour 2019;5(2): 118-129 Homepage: http://sc.journals.umz.ac.ir/ DOI: 10.22080/jgr.2019.2408 RESEARCH ARTICLE Phylogenetic Assessment of Some Species of Crocus Genus Using DNA Barcoding Fatemeh Aghighiravan, Majid Shokrpour*, Vahideh Nazeri and Mohammad-Reza Naghavi Department of Horticulture, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran A R T I C L E I N F O A B S T R A C T Article history: DNA barcoding is a simple method for the identification of any species using a Received 14 April 2019 short genetic sequence from a standard genome section. The present study Accepted 25 June 2019 aimed at examining the nuclear and chloroplast diversity as well as the Available online 12 July 2019 phylogenetic relationships of eight species of saffron including four spring- flowering and five autumn-flowering species from different parts of Iran, using Keywords: the nuclear barcode and chloroplast genes to specify their evolutionary Saffron pathway and to examine their genetic affinity. First, PCR was performed using Wild species the primers designed based on the chloroplast barcodes of matK, trnL, and PCR rbcL, and nuclear barcode of ITS. Then, the PCR products were purified and Cluster analysis DNA marker sequenced. The results of the phylogenetic tree indicated that the examined saffron species of Iran were separated from each other based on the sequences *Corresponding author: of ITS, matK, and trnL barcodes. In the rbcL barcode, the relationships of a M, Shokrpour number of species remained unresolved, and this genetic region could not [email protected] appropriately discriminate the species. -
Forma Nov.: a New Peloric Orchid from Ibaraki Prefecture, Japan
The Japanese Society for Plant Systematics ISSN 1346-7565 Acta Phytotax. Geobot. 65 (3): 127–139 (2014) Cephalanthera falcata f. conformis (Orchidaceae) forma nov.: A New Peloric Orchid from Ibaraki Prefecture, Japan 1,* 1 1 HirosHi Hayakawa , CHiHiro Hayakawa , yosHinobu kusumoto , tomoko 1 1 2 3,4 nisHida , Hiroaki ikeda , tatsuya Fukuda and Jun yokoyama 1National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan. *[email protected] (author for correspondences); 2Faculty of Agriculture, Kochi University, Nan- koku, Kochi 783-8502, Japan; 3Faculty of Science, Yamagata University, Kojirakawa, Yamagata 990-8560, Japan; 4Institute for Regional Innovation, Yamagata University, Kaminoyama, Yamagata 999-3101, Japan We recognize a new peloric form of the orchid Cephalanthera falcata (Thunb.) Blume f. conformis Hi- ros. Hayak. et J. Yokoy., which occurs in the vicinity of the Tsukuba mountain range in Ibaraki Prefec- ture, Japan. This peloric form is sympatric with C. falcata f. falcata. The peloric flowers have a petal-like lip. The flowers are radially symmetrical and the perianth parts spread weakly compared to normal flow- ers. The stigmas are positioned at the column apex, thus neighboring stigmas and the lower parts of the pollinia are conglutinated. We observed similar vegetative traits among C. falcata f. falcata, C. falcata f. albescens S. Kobayashi, and C. falcata f. conformis. The floral morphology in C. falcata f. conformis resembles that of C. nanchuanica (S.C. Chen) X.H. Jin et X.G. Xiang (i.e., similar petal-like lip and stig- ma position at the column apex; syn. Tangtsinia nanchuanica S.C. -
Phenolic Profiles, Antimicrobial and Antioxidant Activity of the Various Extracts of Crocus Species in Anatolia
African Journal of Microbiology Research Vol. 4 (11) pp. 1154-1161, 4 June, 2010 Available online http://www.academicjournals.org/ajmr ISSN 1996-0808 © 2010 Academic Journals Full Length Research Paper Phenolic profiles, antimicrobial and antioxidant activity of the various extracts of Crocus species in Anatolia Gulumser Acar1*, Nazime Mercan Dogan1, Mehmet Emin Duru2 and Ibrahim Kıvrak2 1Department of Biology, Faculty of Science and Arts, Pamukkale University, 20017, Kinikli, Denizli, Turkey. 2Department of Chemistry, Faculty of Science and Arts, Mula University, 48000, Mula, Turkey. Accepted 6 May, 2010 The phenolic profile and quantitative composition of methanol extracts of Crocus baytopiorum which was endemic species in Denizli, Turkey was detected by high performance liquid chromatography (HPLC-DAD). The HPLC analysis of phenolic compounds in methanol extract of C. baytopiorum showed that p-Coumaric acid, apigenin-glucoside, rosmarinic acid, quercetin and kampferol were present. Also, the methanol, ethyl acetate and hexane extracts from Crocus biflorus, C. baytopiorum and Crocus flavus subp. dissectus were investigated for their in vitro antimicrobial and antioxidant activities in the present study. Ethyl acetate and methanol extracts have demonstrated significant antimicrobial activities against tested micro organisms Escherichia coli ATCC 35218, Pseudomonas aeruginosa NRRL B-23, Klebsiella pneumoniae ATCC 27736, Yersinia enterecolitica RSKK 1501, Proteus vulgaris RSKK 96026, Bacillus cereus RSKK 863, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Micrococcus luteus NRRL B-4375 and Candida albicans (clinical isolate). The methanol extract of C. flavus subsp. dissectus had maximum activities on Yersinia enterocolitica RSKK 1501. Minimum inhibition concentrations of plant extracts have investigated on Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633 and Bacillus cereus RSKK 863. -
The Real Ponerorchis Nana (King & Pantling) Soó Resurrected
Pleione 10(2): 279 - 282. 2016. ISSN: 0973-9467 © East Himalayan Society for Spermatophyte Taxonomy The real Ponerorchis nana (King & Pantling) Soó resurrected Magnus Lidén1 and Alister Adhikari2 1Uppsala university, EBC: Systematic Biology. Norbyvägen 18D, 75236 Uppsala, Sweden. E-mail: [email protected]. 2 Dr. Graham’s Homes, Kalimpong 734301, West Bengal. E-mail: [email protected]. [Received 01.11.2016; Revised & accepted 04.11.2016; Published 31.12.2016] Abstract We report a find of the rare orchid Ponerorchis nana (King & Pantling) Soó (Orchidaceae) from Lachung, Sikkim, and compare it with the very different species P. chusua with which it has previously been associated. Ponerorchis nana is currently known from East Sikkim Eastwards to Central Arunachal Pradesh, and grows on moss-covered cliffs and tree trunks. It seems closely related to Amitostigma pathakianum. Key words: Ponerorchis nana, Identity, Reestablished species Ponerorchis nana (King & Pantling) Soó (Orchidaceae) is a much misunderstood taxon. In Flora of Bhutan (Pearce & Cribb 2002) and on most websites (see references: web-resources) P. nana is said to be either very similar to or synonymous with P. chusua, and the epithet has been used for both narrow-leaved and broad-leaved small individuals of P. Chusua (e.g. Adhikari 2008). The root of the confusion started long lack when King & Pantling (1898) originally described P. nana as a variety of P. chusua and even hinted at intermediates. However, Ponerorchis nana (Figures 1, 2) is very different from P. chusua (Figure 3), in morphology as well as in ecology, and no intermediates are known. Pantling’s original drawing (King & Pantling 1898) shows most of its distinctive features: small and delicate growth; a single linear arcuate channeled leaf with shortly clasping base; 1- to 2-flowered (very rarely 3-flowered) inflorescence; flowers less than half the size of those of P. -
New Cytotype of Crocus Pallasii Sub Sp
New Cytotype of Crocus pallasii sub sp. haussknechtii from West of Iran M. Sanei, H. Rahimyan Y.M. Agayev, S. Soheilivand Department of Agronomy Agricultural Biotechnology Research Institute University of Mazandaran. Seed and Plant Improvement Institute Sari Mahdasht Road Iran P.O. Box 31535-1897 Karaj Iran Keywords: aceto-iron-hematoxylin, chromosome, Crocus sativus, evolution, karyotype Abstract C. pallasii is one of the close relatives of C. sativus. A new cytotype of this species was discovered from west of Iran (Reno valley & Islamabad-e-Gharb). For karyotype studies, Lewitsky fixative and aceto-iron-hematoxylin stain were used. Karyotype of this cytotype with 2n=12 consists of one large pair of metacentric chromosomes with a satellite in the long arm, one pair of large submetacentrics, two pairs of smaller submetacentrics and two smallest pairs, which are metacentrics. This plant cytogenetically is very similar to C. pallasii subsp. turcicus (2n=12) and also C. pallasii subsp. haussknechtii with 2n=14 chromosomes. However C. p. subsp. haussknechtii with 16 chromosomes is considerably different from these two cytotypes. It seems that during evolution new cytotype with 2n=12 derived from C. p. subsp. haussknechtii with 2n=14. INTRODUCTION The Crocus sativus aggregate (Mathew, 1977) comprises C. niveus., C. sativus., C. cartwrightianus., C. hadriaticus., C. asumaniae., C. oreocreticus., C. thomasii., C. moabiticus, and C. pallasii. One member of the Crocus sativus aggregate is C. pallasii, that's the most widespread of the saffron group species. The area in which it occurs extends from Yugoslavia to Iran and from Crimea to south Jordan, and over this immense area it varies considerably, so much so that a number of the variants have been described as separate species (Mathew, 1982). -
Premium Plant Auction
Upcoming Events August 14 – MOS Monthly Meeting Speaker: Fred Clarke Catasetum, Cycnoches and Mormodes Cymbidiums, Cattleyas, Phalaenopsis, www.SunsetValleyOrchids.com are three related genera from the Tribe Dendrobiums, Epidendrums, and so on. Cymbidieae. Individually, each has Until recently, they have not attracted September 11 – been considered an orchid oddity, or much attention from hybridizers, but in the MOS Annual Plant Auction “botanical,” of interest mainly to the past several years some spectacular Plant list: seriously addicted enthusiast. These hybrids have been produced, receiving massorchid.org/auction plants have a brief deciduous period in great acclaim and an increasingly large winter, when the plants need a definite collection of awards from the American October 9 – dry rest period bordering on neglect. Orchid Society. MOS Monthly Meeting Speaker: Bob Winkley These robust orchids are native to This talk will feature some of the new lowland tropical forests in Central and hybrids and breakthroughs in the Topic: Grooming Plants for Shows South America and are usually grown in hybridization of Catasetum, Cycnoches, warm or intermediate conditions. and Mormodes. November 2-4 – MOS Annual Show Commercially, Catasetum, Cycnoches Fred is offering a 10% discount for pre- “Orchid Jubilee” (or „Swan‟ orchids) and Mormodes (or orders with free shipping. Go to Celebrating 60 Years of MOS „Goblin‟ orchids) have occupied a small www.SunsetValleyOrchids.com to place market niche on the edge of a sea of your order. Next Month: Premium Plant Auction Since the MOS has held 3 auctions in Members are welcome to contribute the last 12 months, the MOS Board special plants, but we ask that they be has made the decision to do pest and disease free. -
Recent Progress in Plant Taxonomy and Floristic Studies in Greece
41 (2): (2017) 123-152 Review article Recent progress in plant taxonomy and floristic studies in Greece Arne Strid1 and Kit Tan 2✳ 1 Bakkevej 6, DK-5853 Ørbæk, Denmark 2 Institute of Biology, University of Copenhagen, Øster Farimagsgade 2D, DK-1353 Copenhagen K, Denmark ABSTraCT: A survey of developments in plant taxonomy, floristics, and phytogeography in Greece during 2005 to 2016 is presented. Species diversity in different areas and on different scales is summarised in five maps. A list of new taxa described during this period is provided, together with their type citations and taxonomic comments where relevant; almost all of the taxa are based on Greek plant material. Keywords: Flora Hellenica, Flora Hellenica Bibliography, Flora Hellenica Database, floristics, phytogeography, species diversity, Greece Received: 31 March 2017 Revision accepted: 10 August 2017 UDC: 581.92+581.96(495)”2005/2016” DOI: 10.5281/zenodo.1026649 INTRODUCTION on literature reports, and the rest on field notes, photographs, etc. Each record in the database generally Flora Hellenica was envisaged to be a comprehensive contains the data provided in a good herbarium label, Flora for the whole of Greece as politically constituted including geographical coordinates (degrees and today. Two volumes were published (Strid & Kit Tan minutes of latitude and longitude). 1997, 2002). Some years after publication of the second In this article, we analyse some of the developments volume, it became apparent that there would not be in Greek plant taxonomy, floristics, and phytogeography the necessary economic resources and manpower to during the period 2005 to 2016, after publication of the complete this series, which was planned to result in nine Bibliography. -
Glasnik Hrvatskog Botaničkog Društva 4(2) 2016
Glasnik Hrvatskog botaničkog društva 4(2) 2016. Glasnik Hrvatskog botaničkog društva Journal of the Croatian Botanical Society Crocus dalmaticus Vis. (Foto: M. Milović) Vol. 4 br. 2 1 Listopad / October 2016. Glasnik Hrvatskog botaničkog društva 4(2) 2016. Glasnik Hrvatskog botaničkog društva Journal of the Croatian Botanical Society Skraćeni naslov / Abbreviated title: Glas. Hrvat. bot. druš. / J. Croat. Bot. Soc. Izdavač / Publisher: Hrvatsko botaničko društvo (www.hbod.hr) / Croatian Botanical Society (www.hbod.hr) Dinamika objavljivanja / Publishing frequency: 3 – 5 brojeva /volumena godišnje / 3 – 5 numbers /volumes annually e-ISSN: 1848-8102 Adresa uredništva / Address: Glasnik Hrvatskog botaničkog društva, Marulićev trg 9a, HR-10000 Zagreb, Hrvatska; fax.: 01 4898 093; tel.: 01 4898 064 / Journal of the Croatian Botanical Society, Marulićev trg 9a, HR-10000 Zagreb, Croatia; fax.: ++385 1 4898 093; tel.: ++385 1 4898 064 Urednici / Editors: Toni Nikolić ([email protected]), Sandro Bogdanović ([email protected]) Asistent urednika / Asistent editors: Igor Boršić Prijelom / Layout and design: Ana Vujasić Urednički odbor / Editorial board: A. Alegro, N. Jasprica, Z. Liber, M. Milović, A. Plenković-Moraj, B. Pevalek-Kozlina, Ž. Škvorc (detalji / details: http://hirc.botanic.hr/Glasnik-HBoD) Teme časopisa Journal topics Vaskularna flora, mahovine, lišajevi, alge, vegetacija, staništa, Vascular plants, mosses, lichens, algae, vegetation, habitats, taksonomija bilja, sistematika bilja, nomenklatura bilja, ekologija plant taxonomy, systematics of plants, nomenclature of plants, bilja, primijenjena botanika ecology of plants, applied botany Ciljevi i obuhvat Objectives and scope Glasnik Hrvatskog botaničkog društva je znanstveni i stručni Journal of the Croatian Botanical Society is the scientific and časopis Hrvatskog botaničkog društva (HBoD). -
A New Species of Amitostigma (Orchidinae, Orchidaceae) from India
Phytotaxa 230 (3): 267–273 ISSN 1179-3155 (print edition) www.mapress.com/phytotaxa/ PHYTOTAXA Copyright © 2015 Magnolia Press Article ISSN 1179-3163 (online edition) http://dx.doi.org/10.11646/phytotaxa.230.3.5 A new species of Amitostigma (Orchidinae, Orchidaceae) from India AVISHEK BHATTACHARJEE Central National Herbarium, Botanical Survey of India, Acharya Jagadish Chandra Bose Indian Botanic Garden, Howrah 711 103, India; e-mail: [email protected] Abstract Amitostigma pathakianum, a new species of the family Orchidaceae is described from Arunachal Pradesh, India. Key words: Amitostigma pathakianum, Amitostigma trifurcatum, Arunachal Pradesh, Ponerorchis Introduction The orchid genus Amitostigma Schlechter (1919: 91) was established by Schlechter based on the type of ‘Mitostigma Blume’ (1856: 189), as it was an illegitimate name [later homonym of Mitostigma Decaisne (1844: 507), Apocynaceae]. Amitostigma is represented by c. 30 species (Pridgeon et al. 2001: 247; Chen et al. 2009: 124), mainly distributed in East Asia and adjacent areas. Most of the species of the genus (c. 22 spp.) are found in China, with 21 endemic species, which suggests the Chinese region as the centre of diversity of Amitostigma. Amitostigma is closely allied to Ponerorchis Reichenbach (1852: 227) and at times it becomes difficult to make clear cut distinction between them and as a result several species have been interchanged between these two genera. Amitostigma can be distinguished from Ponerorchis by having naked viscidia (viscidia enclosed in bursicle in Ponerorchis) and two slightly raised lateral stigma lobes along with a small central lobe (one concave stigma lobe in Ponerorchis). However, exceptions have been recorded in a few species within both genera.