Unit 6-Nucleus
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For the Safe Delivery of Essential Proteins
Dedicated ‘Bodyguards’ for the Safe Delivery of Essential Proteins Dr Brigitte Pertschy DEDICATED ‘BODYGUARDS’ FOR THE SAFE DELIVERY OF ESSENTIAL PROTEINS Ribosomes are undoubtedly one of the most essential cellular components in life. These macromolecules are responsible for the synthesis of proteins in all living cells. Dr Brigitte Pertschy, Dr Ingrid Rössler and Jutta Hafner at the Institute of Molecular Biosciences at the University of Graz, Austria, have discovered that the safe delivery of essential ribosomal proteins that make up the ribosomes is dependant on ‘private bodyguards’ or ‘chaperones’. Nascent Ribosomal Proteins Journey Ribosome synthesis is an important of their synthesis and proper folding Across the Cell to the Nucleus and continuous process. Dr Pertschy of the proteins. Importins have also describes that a growing cell requires been reported as aides in the import of The ribosome is the intricate nano- up to 1,000 ribosomes to be synthesised proteins to the cell nucleus as well as in machinery that translates messenger per minute. The r-proteins are protecting proteins from aggregation. RNA strands (mRNA) into protein. Our produced in the cell cytoplasm by the DNA holds the instructions for building ribosome itself (that way, the ribosome The team speculated that since every protein needed for our bodies to participates in its own reproduction). r-proteins are produced at extremely function. Initially, DNA is transcribed From there the r-proteins must travel high amounts and their correct into mRNA, which contains the amino to the cell nucleus where in a complex functioning is so critical for a cell, these acid sequence of a particular protein. -
Building the Interphase Nucleus: a Study on the Kinetics of 3D Chromosome Formation, Temporal Relation to Active Transcription, and the Role of Nuclear Rnas
University of Massachusetts Medical School eScholarship@UMMS GSBS Dissertations and Theses Graduate School of Biomedical Sciences 2020-07-28 Building the Interphase Nucleus: A study on the kinetics of 3D chromosome formation, temporal relation to active transcription, and the role of nuclear RNAs Kristin N. Abramo University of Massachusetts Medical School Let us know how access to this document benefits ou.y Follow this and additional works at: https://escholarship.umassmed.edu/gsbs_diss Part of the Bioinformatics Commons, Cell Biology Commons, Computational Biology Commons, Genomics Commons, Laboratory and Basic Science Research Commons, Molecular Biology Commons, Molecular Genetics Commons, and the Systems Biology Commons Repository Citation Abramo KN. (2020). Building the Interphase Nucleus: A study on the kinetics of 3D chromosome formation, temporal relation to active transcription, and the role of nuclear RNAs. GSBS Dissertations and Theses. https://doi.org/10.13028/a9gd-gw44. Retrieved from https://escholarship.umassmed.edu/ gsbs_diss/1099 Creative Commons License This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License This material is brought to you by eScholarship@UMMS. It has been accepted for inclusion in GSBS Dissertations and Theses by an authorized administrator of eScholarship@UMMS. For more information, please contact [email protected]. BUILDING THE INTERPHASE NUCLEUS: A STUDY ON THE KINETICS OF 3D CHROMOSOME FORMATION, TEMPORAL RELATION TO ACTIVE TRANSCRIPTION, AND THE ROLE OF NUCLEAR RNAS A Dissertation Presented By KRISTIN N. ABRAMO Submitted to the Faculty of the University of Massachusetts Graduate School of Biomedical Sciences, Worcester in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSPOPHY July 28, 2020 Program in Systems Biology, Interdisciplinary Graduate Program BUILDING THE INTERPHASE NUCLEUS: A STUDY ON THE KINETICS OF 3D CHROMOSOME FORMATION, TEMPORAL RELATION TO ACTIVE TRANSCRIPTION, AND THE ROLE OF NUCLEAR RNAS A Dissertation Presented By KRISTIN N. -
Introduction to the Cell Cell History Cell Structures and Functions
Introduction to the cell cell history cell structures and functions CK-12 Foundation December 16, 2009 CK-12 Foundation is a non-profit organization with a mission to reduce the cost of textbook materials for the K-12 market both in the U.S. and worldwide. Using an open-content, web-based collaborative model termed the “FlexBook,” CK-12 intends to pioneer the generation and distribution of high quality educational content that will serve both as core text as well as provide an adaptive environment for learning. Copyright ©2009 CK-12 Foundation This work is licensed under the Creative Commons Attribution-Share Alike 3.0 United States License. To view a copy of this license, visit http://creativecommons.org/licenses/by-sa/3.0/us/ or send a letter to Creative Commons, 171 Second Street, Suite 300, San Francisco, California, 94105, USA. Contents 1 Cell structure and function dec 16 5 1.1 Lesson 3.1: Introduction to Cells .................................. 5 3 www.ck12.org www.ck12.org 4 Chapter 1 Cell structure and function dec 16 1.1 Lesson 3.1: Introduction to Cells Lesson Objectives • Identify the scientists that first observed cells. • Outline the importance of microscopes in the discovery of cells. • Summarize what the cell theory proposes. • Identify the limitations on cell size. • Identify the four parts common to all cells. • Compare prokaryotic and eukaryotic cells. Introduction Knowing the make up of cells and how cells work is necessary to all of the biological sciences. Learning about the similarities and differences between cell types is particularly important to the fields of cell biology and molecular biology. -
SMC Complexes Orchestrate the Mitotic Chromatin Interaction Landscape
Curr Genet DOI 10.1007/s00294-017-0755-y REVIEW SMC complexes orchestrate the mitotic chromatin interaction landscape Yasutaka Kakui1 · Frank Uhlmann1 Received: 13 September 2017 / Revised: 14 September 2017 / Accepted: 16 September 2017 © The Author(s) 2017. This article is an open access publication Abstract Chromatin is a very long DNA–protein complex Keywords Chromosome condensation · SMC complex · that controls the expression and inheritance of the genetic Chromatin · Cell cycle · Hi-C information. Chromatin is stored within the nucleus in interphase and further compacted into chromosomes dur- ing mitosis. This process, known as chromosome condensa- Introduction tion, is essential for faithful segregation of genomic DNA into daughter cells. Condensin and cohesin, members of How chromatin is spatially organized within the cell nucleus the structural maintenance of chromosomes (SMC) fam- and within chromosomes is a fundamental question in cell ily, are fundamental for chromosome architecture, both biology. Centimeter-long DNA molecules change their spa- for establishment of chromatin structure in the interphase tial chromatin organization within micrometer-sized cells nucleus and for the formation of condensed chromosomes during cell cycle progression. In interphase, chromatin is in mitosis. These ring-shaped SMC complexes are thought distributed throughout the nucleus to express the genetic to regulate the interactions between DNA strands by topo- information. When cells enter mitosis, chromatin becomes logically entrapping DNA. How this activity shapes chro- compacted to form mitotic chromosomes. Chromosome mosomes is not yet understood. Recent high throughput condensation, the gross morphological change of spatial chromosome conformation capture studies revealed how chromatin organization in mitosis, is indispensable for chromatin is reorganized during the cell cycle and have the faithful inheritance of genetic information. -
The Splicing Factor XAB2 Interacts with ERCC1-XPF and XPG for RNA-Loop Processing During Mammalian Development
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.20.211441; this version posted July 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The Splicing Factor XAB2 interacts with ERCC1-XPF and XPG for RNA-loop processing during mammalian development Evi Goulielmaki1*, Maria Tsekrekou1,2*, Nikos Batsiotos1,2, Mariana Ascensão-Ferreira3, Eleftheria Ledaki1, Kalliopi Stratigi1, Georgia Chatzinikolaou1, Pantelis Topalis1, Theodore Kosteas1, Janine Altmüller4, Jeroen A. Demmers5, Nuno L. Barbosa-Morais3, George A. Garinis1,2* 1. Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology- Hellas, GR70013, Heraklion, Crete, Greece, 2. Department of Biology, University of Crete, Heraklion, Crete, Greece, 3. Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, Portugal, 4. Cologne Center for Genomics (CCG), Institute for Genetics, University of Cologne, 50931, Cologne, Germany, 5. Proteomics Center, Netherlands Proteomics Center, and Department of Biochemistry, Erasmus University Medical Center, the Netherlands. Corresponding author: George A. Garinis ([email protected]) *: equally contributing authors bioRxiv preprint doi: https://doi.org/10.1101/2020.07.20.211441; this version posted July 21, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract RNA splicing, transcription and the DNA damage response are intriguingly linked in mammals but the underlying mechanisms remain poorly understood. Using an in vivo biotinylation tagging approach in mice, we show that the splicing factor XAB2 interacts with the core spliceosome and that it binds to spliceosomal U4 and U6 snRNAs and pre-mRNAs in developing livers. -
Plant & Animal Cells and Their Organelles
Cells and Their Organelles The cell is the basic unit of life. All cells are surrounded by a cell membrane (which is sometimes called a plasma membrane). The cell membrane is semipermeable, meaning it allows some substances to pass into the cell and blocks others out. Plant cells have an additional layer surrounding them called the cell wall. The cell wall is made of nonliving material called cellulose. The centriole (also called the "microtubule organizing center") is a small body located near the nucleus. The centriole is where microtubules are made. During cell division (mitosis), the centriole divides and the two parts move to opposite sides of the dividing cell. Only animal cells have centrosomes. Microtubules are shaped like soda straws and give the nucleus and cell its shape (like a skeleton gives you shape) Answer the questions after each reading section on your own paper, please! 1. What is the basic unit of living things? 2. What surrounds all cells? 3. What is meant by semipermeable? 4. What additional layer is found around the outside of plant cells? 5. Cell walls in plants are made up of C_ ___ ___ ___ ___ ___ ___ ___ ___. 6. Centrioles are found inside of what type of cell only? 7. Microtubules have what shape and do what jobs for the cell? __________________________________________________________________________________________ The nucleus (in the center of a cell) is a rounded body containing the nucleolus and a cell’s DNA. The nucleus controls most of the functions of the cell by controlling protein synthesis. The nucleus of plant and animal cells is surrounded by the nuclear membrane. -
DNA Damage Alters Nuclear Mechanics Through Chromatin Reorganisation
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.10.197517; this version posted July 11, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. DNA damage alters nuclear mechanics through chromatin reorganisation Ália dos Santos1, Alexander W. Cook1, Rosemarie E Gough1, Martin Schilling2, Nora Aleida Olszok2, Ian Brown3, Lin Wang4, Jesse Aaron5, Marisa L. Martin-Fernandez4, Florian Rehfeldt2,6* and Christopher P. Toseland1* 1Department of Oncology and Metabolism, University of Sheffield, Sheffield, S10 2RX, UK.2University of Göttingen, 3rd Institute of Physics – Biophysics, Göttingen, 37077, Germany. 3School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK. 4Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, Didcot, Oxford OX11 0QX, UK. 5Advanced Imaging Center, HHMI Janelia Research Campus, Ashburn, USA. 6University of Bayreuth, Experimental Physics 1, Bayreuth, 95440, Germany. *Corresponding Authors: Florian Rehfeldt [email protected] & Christopher P. Toseland [email protected] Key words: Mechanics, DNA damage, DNA organisation, Nucleus ABSTRACT Cisplatin, specifically, creates adducts within the DNA double-strand breaks (DSBs) drive genomic double helix, which then lead to double-strand instability. For efficient and accurate repair of breaks (DSBs) in the DNA during replication, these DNA lesions, the cell activates DNA through replication-fork collapse3. damage repair pathways. However, it remains DSBs can result in large genomic aberrations and unknown how these processes may affect the are, therefore, the most deleterious to the cell. -
Cytochemical Features Common to Nucleoli and Cytoplasmic Nucleoloids of Olea Europaea Meiocytes: Detection of Rrna by in Situ Hybridization
Journal of Cell Science 107, 621-629 (1994) 621 Printed in Great Britain © The Company of Biologists Limited 1994 JCS8341 Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes: detection of rRNA by in situ hybridization J. D. Alché, M. C. Fernández and M. I. Rodríguez-García* Plant Biochemistry, Molecular and Cellular Biology Department, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, E- 18008 Granada, Spain *Author for correspondence SUMMARY We used light and electron microscopic techniques to study highly phosphorylated proteins. Immunohistochemical the composition of cytoplasmic nucleoloids during meiotic techniques failed to detect DNA in either structure. In situ division in Olea europaea. Nucleoloids were found in two hybridization to a 18 S rRNA probe demonstrated the clearly distinguishable morphological varieties: one similar presence of ribosomal transcripts in both the nucleolus and in morphology to the nucleolus, and composed mainly of nucleoloids. These similarities in morphology and compo- dense fibrillar component, and another surrounded by sition may reflect similar functionalities. many ribosome-like particles. Cytochemical and immuno- cytochemical techniques showed similar reactivities in nucleoloids and the nucleolus: both are ribonucleoproteic Key words: nucleoloids, nucleolar proteins, rRNA, in situ in nature, and possess argyrophillic, argentaffinic and hybridization INTRODUCTION lentum (Carretero and Rodríguez-García, unpublished observa- tions). The reason for this diversity is unknown. Cytoplasmic bodies similar in morphology and ultrastructural Nucleoloids have rarely been studied in genera other than characteristics to the nucleolus have been reported many times Lilium. Cytoplasmic nucleoloids are very common in Olea in relation to plant meiosis (Latter, 1926; Frankel, 1937; europaea during microsporogenesis and their large size and Hakansson and Levan, 1942; Gavaudan, 1948; Lindemann, peculiar morphological characteristics make them a good 1956). -
Nucleolus: a Central Hub for Nuclear Functions Olga Iarovaia, Elizaveta Minina, Eugene Sheval, Daria Onichtchouk, Svetlana Dokudovskaya, Sergey Razin, Yegor Vassetzky
Nucleolus: A Central Hub for Nuclear Functions Olga Iarovaia, Elizaveta Minina, Eugene Sheval, Daria Onichtchouk, Svetlana Dokudovskaya, Sergey Razin, Yegor Vassetzky To cite this version: Olga Iarovaia, Elizaveta Minina, Eugene Sheval, Daria Onichtchouk, Svetlana Dokudovskaya, et al.. Nucleolus: A Central Hub for Nuclear Functions. Trends in Cell Biology, Elsevier, 2019, 29 (8), pp.647-659. 10.1016/j.tcb.2019.04.003. hal-02322927 HAL Id: hal-02322927 https://hal.archives-ouvertes.fr/hal-02322927 Submitted on 18 Nov 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Nucleolus: A Central Hub for Nuclear Functions Olga Iarovaia, Elizaveta Minina, Eugene Sheval, Daria Onichtchouk, Svetlana Dokudovskaya, Sergey Razin, Yegor Vassetzky To cite this version: Olga Iarovaia, Elizaveta Minina, Eugene Sheval, Daria Onichtchouk, Svetlana Dokudovskaya, et al.. Nucleolus: A Central Hub for Nuclear Functions. Trends in Cell Biology, Elsevier, 2019, 29 (8), pp.647-659. 10.1016/j.tcb.2019.04.003. hal-02322927 HAL Id: hal-02322927 https://hal.archives-ouvertes.fr/hal-02322927 Submitted on 18 Nov 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. -
Mitochondria in the Nuclei of Rat Myocardial Cells
cells Article Mitochondria in the Nuclei of Rat Myocardial Cells Chupalav M. Eldarov 1, Irina M. Vangely 1, Valeriya B. Vays 1, Eugene V. Sheval 1, Susanne Holtze 2 , Thomas B. Hildebrandt 2, Natalia G. Kolosova 3 , Vasily A. Popkov 1, Egor Y. Plotnikov 1 , Dmitry B. Zorov 1, Lora E. Bakeeva 1 and Vladimir P. Skulachev 1,4,* 1 A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia; [email protected] (C.M.E.); [email protected] (I.M.V.); [email protected] (V.B.V.); [email protected] (E.V.S.); [email protected] (V.A.P.); [email protected] (E.Y.P.); [email protected] (D.B.Z.); [email protected] (L.E.B.) 2 Department of Reproduction Management, Leibniz-Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany; [email protected] (S.H.); [email protected] (T.B.H.) 3 Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia; [email protected] 4 Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119992 Moscow, Russia * Correspondence: [email protected]; Tel.: +7-495-939-55-30 Received: 12 December 2019; Accepted: 5 March 2020; Published: 14 March 2020 Abstract: Electron microscopic study of cardiomyocytes taken from healthy Wistar and OXYS rats and naked mole rats (Heterocephalus glaber) revealed mitochondria in nuclei that lacked part of the nuclear envelope. The direct interaction of mitochondria with nucleoplasm is shown. The statistical analysis of the occurrence of mitochondria in cardiomyocyte nuclei showed that the percentage of nuclei with mitochondria was roughly around 1%, and did not show age and species dependency. -
Condensed DNA: Condensing the Concepts
Progress in Biophysics and Molecular Biology 105 (2011) 208e222 Contents lists available at ScienceDirect Progress in Biophysics and Molecular Biology journal homepage: www.elsevier.com/locate/pbiomolbio Review Condensed DNA: Condensing the concepts Vladimir B. Teif a,b,*, Klemen Bohinc c,d a BioQuant and German Cancer Research Center, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany b Institute of Bioorganic Chemistry, Belarus National Academy of Sciences, Kuprevich 5/2, 220141, Minsk, Belarus c Faculty of Health Sciences, Zdravstvena pot 5, 1000 Ljubljana, Slovenia d Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, 1000 Ljubljana, Slovenia article info abstract Article history: DNA is stored in vivo in a highly compact, so-called condensed phase, where gene regulatory processes Available online 16 July 2010 are governed by the intricate interplay between different states of DNA compaction. These systems often have surprising properties, which one would not predict from classical concepts of dilute solutions. The Keywords: mechanistic details of DNA packing are essential for its functioning, as revealed by the recent devel- DNA condensation opments coming from biochemistry, electrostatics, statistical mechanics, and molecular and cell biology. Ligand binding Different aspects of condensed DNA behavior are linked to each other, but the links are often hidden in Counterion correlations the bulk of experimental and theoretical details. Here we try to condense some of these concepts and Macromolecular crowding fi Chromatin provide interconnections between the different elds. After a brief description of main experimental Gene regulation features of DNA condensation inside viruses, bacteria, eukaryotes and the test tube, main theoretical approaches for the description of these systems are presented. -
Functional Proximity Mapping of RNA Binding Proteins Uncovers a Mitochondrial Mrna Anchor That Promotes Stress Recovery
Functional proximity mapping of RNA binding proteins uncovers a mitochondrial mRNA anchor that promotes stress recovery Wei Qin Stanford University Samuel Myers Broad Institute Dominique Carey The Broad Institute of MIT and Harvard Steven Carr Broad Institute Alice Ting ( [email protected] ) Stanford University https://orcid.org/0000-0002-8277-5226 Article Keywords: Unbiased Proteome Discovery, Nucleolus, Outer Mitochondrial Membrane, Local Translation, Functional Protein Mapping Posted Date: November 12th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-103196/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/49 Abstract Proximity labeling (PL) with genetically-targeted promiscuous enzymes has emerged as a powerful tool for unbiased proteome discovery. By combining the spatiotemporal specicity of PL with methods for functional protein enrichment, it should be possible to map specic protein subclasses within distinct compartments of living cells. Here we demonstrate this capability for RNA binding proteins (RBPs), by combining peroxidase-based PL with organic-aqueous phase separation of crosslinked protein-RNA complexes (“APEX-PS”). We validated APEX-PS by mapping nuclear RBPs, then applied it to uncover the RBPomes of two unpuriable subcompartments - the nucleolus and the outer mitochondrial membrane (OMM). At the OMM, we discovered the RBP SYNJ2BP, which retains specic nuclear-encoded mitochondrial mRNAs during translation stress, to promote their local translation and import of protein products into the mitochondrion during stress recovery. APEX-PS is a versatile tool for compartment- specic RBP discovery and expands the scope of PL to functional protein mapping. Introduction RNA-protein interactions are pervasive in both transient and stable macromolecular complexes underlying transcription, translation and stress response 1, 2.