DOCSLIB.ORG

Microsatellite Genetic Differentiation Between Two Populations of European Catfish (Silurus Glanis) in Iran

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Microsatellite Genetic Differentiation Between Two Populations of European Catfish (Silurus Glanis) in Iran Journal of Survey in Fisheries Sciences 7(2) 123-132 2021 Microsatellite genetic differentiation between two populations of European catfish (Silurus glanis) in Iran Behmanesh Sh.1; Amiri A.2; Yarmohammadi M.3; Golshan M.4* Received: April 2020 Accepted: October 2020 Abstract In the present study the population genetic structure of European catfish in the Anzali Lagoon and Aras Lake were examined using microsatellite markers. Sixty fin clip samples of Silurus glanis from two regions were collected and for genetic analysis 6 microsatellite loci were used to assess the population genetic structure of S. glanis. There were significant differences based on average number of alleles per locus and heterozygosity between two populations (p<0.01). The observed heterozygosity (Ho) for each population (He) per locus was from 0.297 to 0.733 in Aras lake and Anzali lagoon samples, respectively.The Analysis of molecular variance (AMOVA) indicated that the proportion of the genetic variation attributed to differences among populations of the S. glanis was highly significant for both FST and RST (FST = 0.165, RST = 0.38, p<0.001). Excess or lacks of heterozygosity was observed but most of used microsatellite loci in selected areas were at Hardy-Weinberg equilibrium. Our finding showed the two populations are genetically separated, therefore fisheries management and restocking program of this species especially in Anzali Lagoon is recommended. Keywords: European Catfish, Genetic population, microsatellite markers Downloaded from sifisheriessciences.com at 11:34 +0330 on Thursday September 30th 2021 [ DOI: 10.18331/SFS2021.7.2.10 ] 1-Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandare Anzali, Iran 2- Department of Biology, Faculty of Fisheries Sciences Science, Ahvaz branch, Islamic Azad University, Ahvaz, Iran 3-International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran 4-Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran *Corresponding author's Email: [email protected] 124 Behmanesh et al., Microsatellite genetic differentiation between two populations of European … Introduction Although, allozyme (Triantafyllidis et Diversity plays important roles in al., 1999a) and mitochondrial DNA populations due to provide necessary (mtDNA) (Triantafyllidis et al., 1999b; spectrum of genotypes for adaptive Krieg et al., 2000) were examined to response to change environmental study population genetic of S. glanis, conditions (Webster et al., 2018). neither of them were able to reveal high Heterozygous individuals usually are levels of genetic variability to separate superior to less heterozygous wild and hatchery populations. Krieg et individuals in characteristics such as al. (1999) reported population growth, fertility, and disease resistance differentiation among different species (Beardmore et al., 1997). of Siluridae family using microsatellite Microsatellites are known as a simple markers. Triantafyllidis et al. (2002) sequence repeats (SSRs), simple showed that the genetic diversity was sequence length polymorphisms much higher than previous allozyme (SSLPs), or short tandem repeats and mtDNA studied on this species by (STR), are regions of DNA that exhibit using 10 microsatellite markers in short repetitive sequence motifs. These different areas. Quan et al. (2006) motifs are often composed of 1-6 base examined population structure and pair repeat sequences. Microsatellites genetic variation of northern sheatfish have been used as genetic markers to (S. soldatovi) in wild and farmed assess population genetic structure, populations using microsatellite broodstock identify for management in markers. Results showed that Ho and He hatcheries, evolutionary biology, and other heterozygosity parameters conservation biology and genetic were not different between populations. mapping (Triantafyllidist et al., 2002). Bahrami Kamangar et al. (2015) European catfish has global distribution investigated the genetic diversity and as a native or an introduced species genetic structure of four populations of (Cucherousset et al., 2018). Silurus Silurus glanis by microsatellite DNA glanis is well known as an economic markers. species and an important for S. glanis is one of the native species aquaculture industry (Rees et al., 2017). of Iran where mainly distributed in There are large number of studies have Anzali Lagoon and Aras Lake, north been conducted on reproductive cycles, and northwest of Iran. Due to genome manipulation and biology of overfishing and poor fisheries Downloaded from sifisheriessciences.com at 11:34 +0330 on Thursday September 30th 2021 [ DOI: 10.18331/SFS2021.7.2.10 ] gametes of S. glanis (Legendre et al., management, the capture production 1996; Smitherman et al., 1996; has been declined over the last decades. Varkonyi et al., 1998; Brzuska and Thus, the present study aimed to assess Adamek, 1999; Bolliet et al., 2001; genetic diversity of Iranian populations Kumar et al., 2017; Zibiene and Zibas, of S. glanis in Anzali Lagoon and Aras 2019; Linhart et al., 2020) but study of Lake regions using microsatellite DNA population genetic structure is rare. markers. Journal of Survey in Fisheries Sciences 7(2) 2021 125 Materials and methods some modifies in regents and annealing Sample collections and DNA extraction temperature. Six microsatellite loci for Sixty individuals of S. glanis were S. glanis were used and all of the loci obtained from two selected sites, Anzali have been described by Krieg et al. Lagoon (37°28′22″N 49°27′44″E) and (1999). Detailed information of these Aras Lake (39°05′28″N 45°24′08″E) microsatellite loci such reference, through trap and fin clips were taken forward and reverse primers, fragment and placed in 100% ethanol size and annealing temperature was immediately. Total DNA was extracted described in Table 1. The PCR from each individual using standard amplification was performed in a final SDS proteinase-K digestion; phenol: volume of 25 µL, containing 100 ng chloroform: isoamylalcohol extraction template DNA, 10 mM forward and and ethanol precipitation as described in reverse primers, 200 µm of dNTPs, 0.5 Hillis et al. (1996). The quality and u/µL of taq DNA polymerase (Cinagen, quantity of extracted DNA and Iran), 50 mM MgCl2, 10X PCR buffer Polymerase chain reaction (PCR) was and distilled water using BIOER examined by 1% agarose gel thermal cycler (XP cycler gradient, 96 electrophoresis and nanodrop plus, Bioer, China) under the following spectrophotometery (ND 1000, USA) conditions: initial denaturation of 4 min respectively. at 94ºC (primary denaturation) followed by 30 cycles of 30 s denaturation at PCR profiles and primer sequences 94ºC, 60 s at the respective annealing The PCR amplifications were done temperature, and 60 s extension at 72ºC, according to Krieg et al. (1999) with finishing with 10 min at 72oC as the elongation period. Table 1: Information of 6 polymorphic microsatellite loci used in the present study. Annealing Locus Primer (5’-3’) Size (bp) Reference temp. (ºC) F-CCACTTATGCACCTGAAGG Sgl33INRA R-GGCCAATTAAACAGGTACAG 150-200 58 F-CCAATTTACCTCAGACTACTTCTG Sgl5fINRA R-GCACGTGCAAAGTCCTG 125-210 55 F-CTTTGGTGAGTCAGAAACACG Sgl695INRA R-GCACTACTGGTAGATGCT 180-250 56 Krieg et al. (1999) F-CTGCTCAATCAAAGTTGGTTC Downloaded from sifisheriessciences.com at 11:34 +0330 on Thursday September 30th 2021 [ DOI: 10.18331/SFS2021.7.2.10 ] Sgl7159INRA R-CAAACTAAGTTCAGCCAGGC 220-300 55 F-GTGAATGTGCTTTAAGGGC Sgl7eINRA R-GTTCATGGTGTCACTGCG 200-300 59 F-GGCTGTATGTTAAGTTATTTTCAG Sgl7fINRA 220-300 60 R-CTGAGCAGTGGCCAGAATG 126 Behmanesh et al., Microsatellite genetic differentiation between two populations of European … Gel electrophoresis and statistical (Cleaver Scientific Ltd, UK). Gel run analyses was carried out at 120 V until the The PCR products were separated by loading buffer reached the bottom of electrophoresis through 6% (w/v) the plate. After electrophoresis, the gel polyacrylamide gel (29:1 acrylamide: was stained with a silver nitrate bis acrylamide; 1x TBE buffer) using a protocol (Fig. 1). Cleaver gel electrophoresis system Figure 1: Polyacrylamide electrophoresis patterns of Silurus glanis in the locus Sg133INRA. Microsatellite alleles were identified by examine the partition of variance their size in base pairs (Peakall and between and within two populations. Smouse, 2006). Allele lengths were estimated by comparison with a 100 bp Results DNA marker ladder (Fermentas GmbH, PCR amplification Germany). Measures of genetic Eight microsatellite loci were diversity were determined for each successfully amplified where two sets population including, number of alleles (Sgl310INRA and Sgl325INRA) per locus, observed heterozygosity (Ho), showed monomorphic bands in two expected heterozygosity (He) and populations and six sets produced deviations from Hardy-Weinberg polymorphic bands (Table 2). Locus equilibrium (HWE) between pairwise Sgl5fINRA in samples of Anzali loci. The genetic distance between Lagoon showed highest number of populations of two areas was estimated alleles (12) and locus Sgl7eINRA in using the Nei’s standard genetic samples of Aras Lake presented the distance index (Nei, 1972). The lowest (4) (Table 2). pairwise differentiation between populations of the two areas was also Genetic variations of microsatellite loci Downloaded from
Load more

Recommended publications
  • Fresh- and Brackish-Water Cold-Tolerant Species of Southern Europe: Migrants from the Paratethys That Colonized the Arctic
    water Review Fresh- and Brackish-Water Cold-Tolerant Species of Southern Europe: Migrants from the Paratethys That Colonized the Arctic Valentina S. Artamonova 1, Ivan N. Bolotov 2,3,4, Maxim V. Vinarski 4 and Alexander A. Makhrov 1,4,* 1 A. N. Severtzov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] 2 Laboratory of Molecular Ecology and Phylogenetics, Northern Arctic Federal University, 163002 Arkhangelsk, Russia; [email protected] 3 Federal Center for Integrated Arctic Research, Russian Academy of Sciences, 163000 Arkhangelsk, Russia 4 Laboratory of Macroecology & Biogeography of Invertebrates, Saint Petersburg State University, 199034 Saint Petersburg, Russia; [email protected] * Correspondence: [email protected] Abstract: Analysis of zoogeographic, paleogeographic, and molecular data has shown that the ancestors of many fresh- and brackish-water cold-tolerant hydrobionts of the Mediterranean region and the Danube River basin likely originated in East Asia or Central Asia. The fish genera Gasterosteus, Hucho, Oxynoemacheilus, Salmo, and Schizothorax are examples of these groups among vertebrates, and the genera Magnibursatus (Trematoda), Margaritifera, Potomida, Microcondylaea, Leguminaia, Unio (Mollusca), and Phagocata (Planaria), among invertebrates. There is reason to believe that their ancestors spread to Europe through the Paratethys (or the proto-Paratethys basin that preceded it), where intense speciation took place and new genera of aquatic organisms arose. Some of the forms that originated in the Paratethys colonized the Mediterranean, and overwhelming data indicate that Citation: Artamonova, V.S.; Bolotov, representatives of the genera Salmo, Caspiomyzon, and Ecrobia migrated during the Miocene from I.N.; Vinarski, M.V.; Makhrov, A.A.
    [Show full text]
  • Diversity and Risk Patterns of Freshwater Megafauna: a Global Perspective
    Diversity and risk patterns of freshwater megafauna: A global perspective Inaugural-Dissertation to obtain the academic degree Doctor of Philosophy (Ph.D.) in River Science Submitted to the Department of Biology, Chemistry and Pharmacy of Freie Universität Berlin By FENGZHI HE 2019 This thesis work was conducted between October 2015 and April 2019, under the supervision of Dr. Sonja C. Jähnig (Leibniz-Institute of Freshwater Ecology and Inland Fisheries), Jun.-Prof. Dr. Christiane Zarfl (Eberhard Karls Universität Tübingen), Dr. Alex Henshaw (Queen Mary University of London) and Prof. Dr. Klement Tockner (Freie Universität Berlin and Leibniz-Institute of Freshwater Ecology and Inland Fisheries). The work was carried out at Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Germany, Freie Universität Berlin, Germany and Queen Mary University of London, UK. 1st Reviewer: Dr. Sonja C. Jähnig 2nd Reviewer: Prof. Dr. Klement Tockner Date of defense: 27.06. 2019 The SMART Joint Doctorate Programme Research for this thesis was conducted with the support of the Erasmus Mundus Programme, within the framework of the Erasmus Mundus Joint Doctorate (EMJD) SMART (Science for MAnagement of Rivers and their Tidal systems). EMJDs aim to foster cooperation between higher education institutions and academic staff in Europe and third countries with a view to creating centres of excellence and providing a highly skilled 21st century workforce enabled to lead social, cultural and economic developments. All EMJDs involve mandatory mobility between the universities in the consortia and lead to the award of recognised joint, double or multiple degrees. The SMART programme represents a collaboration among the University of Trento, Queen Mary University of London and Freie Universität Berlin.
    [Show full text]
  • Amur Fish: Wealth and Crisis
    Amur Fish: Wealth and Crisis ББК 28.693.32 Н 74 Amur Fish: Wealth and Crisis ISBN 5-98137-006-8 Authors: German Novomodny, Petr Sharov, Sergei Zolotukhin Translators: Sibyl Diver, Petr Sharov Editors: Xanthippe Augerot, Dave Martin, Petr Sharov Maps: Petr Sharov Photographs: German Novomodny, Sergei Zolotukhin Cover photographs: Petr Sharov, Igor Uchuev Design: Aleksey Ognev, Vladislav Sereda Reviewed by: Nikolai Romanov, Anatoly Semenchenko Published in 2004 by WWF RFE, Vladivostok, Russia Printed by: Publishing house Apelsin Co. Ltd. Any full or partial reproduction of this publication must include the title and give credit to the above-mentioned publisher as the copyright holder. No photographs from this publication may be reproduced without prior authorization from WWF Russia or authors of the photographs. © WWF, 2004 All rights reserved Distributed for free, no selling allowed Contents Introduction....................................................................................................................................... 5 Amur Fish Diversity and Research History ............................................................................. 6 Species Listed In Red Data Book of Russia ......................................................................... 13 Yellowcheek ................................................................................................................................... 13 Black Carp (Amur) ......................................................................................................................
    [Show full text]
  • A Cyprinid Fish
    DFO - Library / MPO - Bibliotheque 01005886 c.i FISHERIES RESEARCH BOARD OF CANADA Biological Station, Nanaimo, B.C. Circular No. 65 RUSSIAN-ENGLISH GLOSSARY OF NAMES OF AQUATIC ORGANISMS AND OTHER BIOLOGICAL AND RELATED TERMS Compiled by W. E. Ricker Fisheries Research Board of Canada Nanaimo, B.C. August, 1962 FISHERIES RESEARCH BOARD OF CANADA Biological Station, Nanaimo, B0C. Circular No. 65 9^ RUSSIAN-ENGLISH GLOSSARY OF NAMES OF AQUATIC ORGANISMS AND OTHER BIOLOGICAL AND RELATED TERMS ^5, Compiled by W. E. Ricker Fisheries Research Board of Canada Nanaimo, B.C. August, 1962 FOREWORD This short Russian-English glossary is meant to be of assistance in translating scientific articles in the fields of aquatic biology and the study of fishes and fisheries. j^ Definitions have been obtained from a variety of sources. For the names of fishes, the text volume of "Commercial Fishes of the USSR" provided English equivalents of many Russian names. Others were found in Berg's "Freshwater Fishes", and in works by Nikolsky (1954), Galkin (1958), Borisov and Ovsiannikov (1958), Martinsen (1959), and others. The kinds of fishes most emphasized are the larger species, especially those which are of importance as food fishes in the USSR, hence likely to be encountered in routine translating. However, names of a number of important commercial species in other parts of the world have been taken from Martinsen's list. For species for which no recognized English name was discovered, I have usually given either a transliteration or a translation of the Russian name; these are put in quotation marks to distinguish them from recognized English names.
    [Show full text]
  • Genetic Polymorphism of Microsatellite DNA in Two Popula- Tions of Northern Sheatfish (Silurus Soldatovi)
    遗 传 学 报 Acta Genetica Sinica, October 2006, 33 (10)：908–916 ISSN 0379-4172 Genetic Polymorphism of Microsatellite DNA in Two Popula- tions of Northern Sheatfish (Silurus soldatovi) QUAN Ying-Chun1,2, SUN Xiao-Wen1,①, LIANG Li-Qun1 1. Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China Abstract: In this article, population variations and genetic structures of two populations of northern sheatfish (Silurus soldatovi) were analyzed using 24 microsatellite loci enriched from southern catfish (S. meriaionalis Chen) by magnetic beads. Gene fre- quency (P), observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information contents (PIC), and number of effective alleles (Ne) were determined. One population was wild, ripe individuals collected from Heilongjiang River (HNS); the other was cultured fry collected from Songhuajiang River (SNS). The Hardy-Weinberg equilibrium (HWE) was tested by the ge- netic departure index (d). The coefficient of gene differentiation GST and ФST by AMOVA (Analysis of Molecular Variety) was im- puted using Arlequin software in this study. In addition, a phylogenetic tree was constructed by UPGMA method based on the pair- wise Nei’s standard distances using PHYLIP. A total of 1 357 fragments with sizes ranging between 102 bp and 385 bp were ac- quired by PCR amplifications. The average number of alleles of the two populations was 8.875. Results indicated that these mi- crosatellite loci were highly polymorphic and could be used as genetic markers. The mean values of the parameters P, Ho, He, PIC, and Ne were 0.165, 0.435, 0.758, 0.742, and 5.019 for HNS and 0.147, 0.299, 0.847, 0.764, and 5.944 for SNS, respectively.
    [Show full text]
  • Cestoidea: Proteocephalidae), with Notes on Four Species of the Genus Proteocephalus, from Japanese Freshwater Fishes
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE Redescription of Paraproteocephalus parasiluri (Yamaguti, 1934) n.comb. (Cestoidea: Proteocephalidae), with notes on four species of the genus Proteocephalus, from Japanese freshwater fishes 著者（英） Takeshi Shimazu journal or Journal of Nagano Prefectural College publication title volume 48 page range 1-9 year 1993-12 URL http://id.nii.ac.jp/1118/00000387/ Creative Commons : 表示 - 非営利 - 改変禁止 http://creativecommons.org/licenses/by-nc-nd/3.0/deed.ja JournalofNaganoPrefecturalCollege，No．48，pp．1－9，December1993 RedescriptionofParaproteocqphalusparasiluri（Yamaguti，1934）mcomb． （Cestoidea：Proteocephalidae），withNotesonFourSpeciesoftheGenus Pro飽Ocqphatui，fromJapaneseFreshwaterFishes TakeshiSHIMAZU＊ Abstract：mYqbroieoc密hah岱1）aYaSihtYi（Yamaguti，1934）n．comb．（＝伽teoc密ha－ hts paYaSilu7i Yamaguti，1934）（Cestoidea：Proteocephalidae）is redescribed and figuredfromthetypemetacestodeandnewadultspecimensfoundinSihmLS aSOねts （Siluridae）fromJapan．Its relationship to nlrqt）γPteOC密hahts parasihlYi（Zmeev， 1936）CheninDubinina，1962，andrelatedproblemsofhomonymyandsynonymyare discussed．AdditionaldataaregivenonProteoc勿hahtsjhtviatilis Bangham，1925，P midoriensis Shimazu，1990，P．plecoglossiYamaguti，1934，and P tetrasiomw（RuM dolphi，1810）Willemse，1965，allfromJapanesefreshwaterfishes． Keywords：Rz77砂7VteOC砂halus parasih17i（Yamaguti，1934）n．comb．，SpeCies of P7Vieo－ C勿hahLS，CeStOdes，freshwaterfishes，Japan． Yamaguti（1934）described a new cestode， Proteoc密hahiSI）amSihlri was borrowed from Pmねoc密血め応 力αクⅦgため，from
    [Show full text]
  • Revision of Isoparorchis Southwell, 1913 (Digenea, Hemiuroidea
    Bull. Natl. Mus. Nat. Sci., Ser. A, 40(1), pp. 15–51, February 22, 2014 Revision of Isoparorchis Southwell, 1913 (Digenea, Hemiuroidea, Isoparorchiidae), Parasites of the Air Bladder of Freshwater Catfishes: a Molecular and Morphological Study Takeshi Shimazu1, Thomas H. Cribb2, Terrence L. Miller3, Misako Urabe4, Nguyen Van Ha5, Tran Thi Binh5 and Marina B. Shed’ko6 1 10486–2 Hotaka-Ariake, Azumino, Nagano 399–8301, Japan E-mail: [email protected] 2 School of Biological Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia 3 School of Marine and Tropical Biology, James Cook University, Cairns, Queensland 4870, Australia 4 Department of Ecosystem Studies, School of Environmental Sciences, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522–8525, Japan 5 Department of Parasitology, Institute of Ecology and Biological Resources, Vietnamese Academy of Sciences and Technology, 18-Hoang Quoc Viet, Hanoi, Vietnam 6 Institution of Russian Academy of Sciences, Institute of Biological and Soil Sciences, Far Eastern Branch of Russian Academy of Sciences, 159 Stoletiya Street, Vladivostok 690022, Russian Federation (Received 5 November 2013; accepted 18 December 2013) Abstract Isoparorchis Southwell, 1913 (Digenea, Hemiuroidea, Isoparorchiidae), parasites of the air bladder of freshwater catfishes (Osteichthyes, Siluriformes) from East, Southeast and South Asia and Australia, is revised based on a molecular and morphological study. Materials, including syntypes where available, were examined from Russia (Primorskiy Kray), Japan, Vietnam, Cam- bodia, Bangladesh, India and Australia. The entire second internal transcribed spacer region of the ribosomal DNA (ITS2 rDNA) was sequenced for 18 distinct samples. Four of the five previously described species are considered to be valid: Isoparorchis trisimilitubis Southwell, 1913 (type spe- cies) from India, I.
    [Show full text]
  • Fishes of Mongolia a Check-List of the fi Shes Known to Occur in Mongolia with Comments on Systematics and Nomenclature
    37797 Public Disclosure AuthorizedPublic Disclosure Authorized Environment and Social Development East Asia and Pacific Region THE WORLD BANK 1818 H Street, N.W. Washington, D.C. 20433, USA Telephone: 202 473 1000 Facsimile: 202 522 1666 E-mail: worldbank.org/eapenvironment worldbank.org/eapsocial Public Disclosure AuthorizedPublic Disclosure Authorized Public Disclosure AuthorizedPublic Disclosure Authorized Fishes of Mongolia A check-list of the fi shes known to occur in Mongolia with comments on systematics and nomenclature Public Disclosure AuthorizedPublic Disclosure Authorized MAURICE KOTTELAT Fishes of Mongolia A check-list of the fi shes known to occur in Mongolia with comments on systematics and nomenclature Maurice Kottelat September 2006 ©2006 Th e International Bank for Reconstruction and Development/THE WORLD BANK 1818 H Street, NW Washington, DC 20433 USA September 2006 All rights reserved. Th is report has been funded by Th e World Bank’s Netherlands-Mongolia Trust Fund for Environmental Reform (NEMO). Some photographs were obtained during diff erent activities and the author retains all rights over all photographs included in this report. Environment and Social Development Unit East Asia and Pacifi c Region World Bank Washington D.C. Contact details for author: Maurice Kottelat Route de la Baroche 12, Case Postale 57, CH-2952 Cornol, Switzerland. Email: [email protected] Th is volume is a product of the staff of the International Bank for Reconstruction and Development/Th e World Bank. Th e fi ndings, interpretations, and conclusions expressed in this paper do not necessarily refl ect the views of the Executive Directors of Th e World Bank or the governments they represent.
    [Show full text]
  • A Review of the Catfish Genus Pterocryptis (Siluridae)
    Journal of Fish Biology (2001) 59, 624–644 doi:10.1006/jfbi.2001.1673, available online at http://www.idealibrary.com on A review of the catfish genus Pterocryptis (Siluridae) in Vietnam, with the description of two new species H. H. N*†¶ J. F‡ *Fish Division, Museum of Zoology, University of Michigan, 1109 Geddes Avenue, Ann Arbor, Michigan 48109-1079, U.S.A., †Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260 and ‡Institut fu¨r Gewa¨ssero¨kologie und Binnenfischerei, Mu¨ggelseedamm 310, D-12561 Berlin, Germany (Received 9 December 2000, Accepted 23 May 2001) Of the silurid catfish genus Pterocryptis in Vietnam, a total of four species are recognized as valid, of which Pterocryptis crenula and P. verecunda are described here as new. The type locality of P. cochinchinensis is restricted to central Vietnam, and the species is redescribed from topotypic material. Pterocryptis crenula (from northeastern Vietnam) can be differentiated from its congeners in having visibly confluent anal and caudal fins while P. verecunda (from Cat Ba Island in northeastern Vietnam) can be differentiated from its congeners in having the genital papilla concealed behind the anus. Notes on the identity of other nominal Pterocryptis species are also provided. 2001 The Fisheries Society of the British Isles Key words: new species; taxonomy; Siluridae; Pterocryptis. INTRODUCTION Silurid catfishes of the genus Pterocryptis Peters, 1861, are usually found in relatively fast-flowing mountain streams throughout India, southern China and South-east Asia. The genus was recently removed from synonymy with Silurus Linnaeus, 1758, by Bornbusch (1991), and the taxonomy of many of its species has not been fully resolved.
    [Show full text]
  • Wels Catfish Silurus Glanis
    Wels catfish Silurus glanis Description Identification Scaleless, elongated body. It can grow up to 13 feet long with a weight of over 880 pounds. Upper side is usually a dark color and the flanks and belly are more pale. Fins are brownish and the body has a mottled appearance that is sometimes accompanied by brown spots. 1 dorsal spone and 4-5 dorsal soft rays, 1 anal spine and 90-94 anal soft rays and a caudal fin with 17 rays. Habitat Native to central, southern and easter Europe and near the Baltic and Caspian Seas. It prefers large, warm lakes and deep, slow-flowing rivers Source: MISIN. 2021. Midwest Invasive Species Information Network. Michigan State University - Applied Spatial Ecology and Technical Services Laboratory. Available online at https://www.misin.msu.edu/facts/detail.php?id=273. where it can remain sheltered in holes, sunken trees, etc. Reproduction Breed annually during spring; hatching takes 3-10 days; reproductive maturity is 4 years for a female and 3 years for a male. The male creates a shallow depression that will hold thousands of eggs. Females can lay up to 30,000 eggs per kilogram of body weight. Impact Have been implicated in declining populations of other commerical fishes. Capable of carrying bacterial disease that can be transmitted to other fish such as Red head disease (Vibrio sp. bacterium) and Gill disease (Flavobacterium). Similar Aristotle's catfish (Silurus aristotelis); Amur catfish (Silurus asotus); Giant lake biwa catfish (Silurus biwaensis); Soldatov's catfish (Silurus soldatovi); Mekong giant catfish (Pangasianodon gigas). Monitoring and Rapid Response Credits The information provided in this factsheet was gathered from the Wikipedia.Individual species images that appear with a number in a black box are courtesy of the Bugwood.org network Source: MISIN.
    [Show full text]
  • 42543039.Pdf
    ENVIRONMENTAL FACTORS AFFECTING THE PATHOGENESIS OF Edwardsiella ictaluri IN STRIPED CATFISH Pangasianodon hypophthalmus (Sauvage) THESIS SUBMITTED TO THE UNIVERSITY OF STIRLING FOR THE DEGREE OF DOCTOR OF PHILOSOPHY BY NGUYEN NGOC PHUOC 31 MARCH 2014 INSTITUTE OF AQUACULTURE Declaration I hereby declare that the work and results presented in this thesis has been composed in its entirety by me. Except where specifically acknowledged, the work described in this thesis has been conducted by me and has not been submitted for any other degree or qualification. Signature: ________________________________________ Signature of supervisor: ________________________________________ Date: ________________________________________ i ABSTRACT Bacillary Necrosis of Pangasius (BNP) caused by Edwardsiella ictaluri is considered to be the most serious disease occurring in farmed striped catfish (Pangasianodon hypophthalmus) in Vietnam. This disease has had an increasing impact over the last ten years and has been reported to cause 50-90% mortality of stocks during a single outbreak. Data obtained from natural outbreaks of E. ictaluri in striped catfish showed the role of environmental factors in the establishment and progression of this disease. At present, factors affecting the virulence and transmission of E. ictaluri in striped catfish are poorly understood. The central hypothesis of this thesis focuses on the complex picture of the environmental factors and infectivity of E. ictaluri in striped catfish. In this study, 80 isolates of E. ictaluri recovered from natural clinical disease outbreaks occurring in striped catfish farms between 2002 and 2011 located in 4 distinct geographical areas within Vietnam were characterised using a variety of methods. The biochemical profiles showed that E. ictaluri isolates from striped catfish in Vietnam have similar phenotypic characteristics to other E.
    [Show full text]
  • The Chromosomes of a Catfish Parasilurus Aristotelis from Greece
    Japanese Journal of Ichthyology 魚 類 学 雑 誌 Vol. 37, No. 2 19 9 0 3 7 巻 2 号 19 9 0 年 The Chromosomes of a Catfish Parasilurus aristotelis from Greece Konstantina Iliadou1 and Brian D. Rackham2 Universityof Patras, Faculty of Natural Science,Department 1 of Biolog y , Section of Animal Biology,GR-26010 Patras, Greec e 2Ministryof Agriculture , Fisheriesand Food, Directorate of Fisheries Researc h, FisheriesLaboratory, Lowestoft, Suffolk,NR 33 OHT, England,U.K. Abstract The karyotype of the catfish, Parasilurus aristotelis, from Lake Trichonis, Greece, shows that the species has a diploid chromosome number of 2n=58. Comparison with published data on the karyotypes of other species belonging to Parasilurus shows the same chromosome number but different arm numbers. Reported karyological data of the European populations of Silurus indicate that they have a diploid number of 2n=60. A reduction in chromosome number is assumed to be connected with speciation and, therefore, Parasilurus probably forms a separate group from that of Silurus. Parasilurus aristotelis (Agassiz, 1856) and Silurus The chromosome preparations were made using glanis Linnaeus, 1758 are the only catfishes found in the method of Kang and Park (1973) and Ojima the freshwaters of Greece (Berg, 1949; Ladiges and (1982). Karyograms were made from photographs Vogt, 1965). The status of Parasilurus is problematic. of 7 very good metaphases and measurements made Haig (1950), in her review of the family Siluridae, of the chromosome arm lengths using calipers to regarded Parasilurus as a junior synonym of Silurus. determine the centromeric positions as described by Her revision was provisional and her conclusions was Levan et al.
    [Show full text]