Elisa Kits and Monoclonal Antibodies for the Complement System

ELISA KITS AND MONOCLONAL ANTIBODIES FOR THE COMPLEMENT SYSTEM

MBL ClassicalÊPathway LectinÊPathway AlternativeÊPathway H-Ficolin KITÊ029 RIGÊ334 HYBÊ131 M-Ficolin ABSÊ036 C1-INH HYBÊ288 L-Ficolin C1q MBL/Ficolin ABSÊ005

C1q B

H2O C3Ê(H2O) FactorÊB C1s C1s C1r MASP-2 D ABSÊ002 MASP-1 HAVÊ005 C3 C3Ê(H O)ÊB 2 FactorÊD Carbohydrate Carbohydrate GAUÊ010 GAUÊ008 C2 C2 C4 C3Ê(H O)ÊBb HYBÊ050 2 Ba FactorÊBa/b HYBÊ008 C3a ProperdinÊ(FactorÊP) C2b C4a C3 Properdin HAVÊ004 HYBÊ0393 HYBÊ005 C4b2a C2a C4b C3bBb C3d HAVÊ003 C4 HYBÊ030 HYBÊ162-04 C3 C3a C3a C4b C3a/C3aÊ(desArg) HYBÊ162-02 GAUÊ013 GAUÊ017 C4b2a3b C3bBb3b

C5Ê-ÊC9 C5 MembraneÊattackÊcomplex HYBÊ029 FactorÊH HYBÊ268 GAUÊ018 C9 GAUÊ020 ABSÊ004 LysisÊorÊphagocytosisÊofÊtheÊmicroorganism C5b-9 FactorÊI DIAÊ011 HYBÊ061

OverviewÊofÊtheÊcomplementÊsystem.ÊTheÊnameÊofÊtheÊcomponentÊandÊBioPortoÊDiagnostics'ÊcatalogÊnumberÊareÊshownÊinÊtheÊblueÊboxes.

REFERENCES IN WHICH ANTIBODYSHOP PRODUCTS HAVE BEEN USED

C2 Cat. no. HYB 050 Rooijakkers SH, Ruyken M, Roos A, Daha MR, Presanis JS, Sim RB, van Wamel WJ, van Kessel KP, van Strijp JA (2005) Immune evasion by a staphylococcal complement inhibitor that acts on C3 convertases. Nat Immunol 6:920-7. Oh KS, Kweon MH, Rhee KH, Ho Lee K, Sung HC (2003) Inhibition of complement activation by recombinant Sh-CRIT-ed1 ana- logues. Immunology 110:73-9. Laich A, Moffatt B, Wong KHN, Hickling TP, Koch C, Sim RB (2001) Purifi cation of second component of human complement, C2, by antibody affi nity chromatography. Intern J Bio-Chromatography 6:151-162. Laich A, Sim RB (2001) Complement C4bC2 complex formation: an investigation by surface plasmon resonance. Biochim Bio- phys Acta 1544:96-112. Stenbaek EI, Koch C, Barkholt V, Welinder KG (1986) Human complement component C2: production and characterization of polyclonal and monoclonal antibodies against C2. Mol Immunol 23:879-886. Cat. no. HAV 003 and HAV 004 004 HAV and 003 HAV no. Cat. C3 C3 Cat. no. HAV 003 and HAV 004 Tang S, Zhou W, Sheerin NS, Vaughan RW, Sacks SH (1999) Contribution of renal secreted complement C3 to the circulating circulating the to C3 complement secreted renal of Contribution (1999) SH Sacks RW, Vaughan NS, Sheerin W, Zhou S, Tang Tang S, Zhou W, Sheerin NS, Vaughan RW, Sacks SH (1999) Contribution of renal secreted complement C3 to the circulating pool in humans. J Immunol 162:4336-41. Immunol J humans. in pool pool in humans. J Immunol 162:4336-41. Naughton MA, Botto M, Carter MJ, Alexander GJ, Goldman JM, Walport MJ (1996) Extrahepatic secreted complement C3 con- C3 complement secreted Extrahepatic (1996) MJ Walport JM, Goldman GJ, Alexander MJ, Carter M, Botto MA, Naughton Naughton MA, Botto M, Carter MJ, Alexander GJ, Goldman JM, Walport MJ (1996) Extrahepatic secreted complement C3 con- tributes to circulating C3 levels in humans. J Immunol 156:3051-6. Immunol J humans. in levels C3 circulating to tributes tributes to circulating C3 levels in humans. J Immunol 156:3051-6. Botto M, Fong KY, So AK, Koch C, Walport MJ (1990) Molecular basis of polymorphisms of human complement component C3. C3. component complement human of polymorphisms of basis Molecular (1990) MJ Walport C, Koch AK, So KY, Fong M, Botto Botto M, Fong KY, So AK, Koch C, Walport MJ (1990) Molecular basis of polymorphisms of human complement component C3. J Exp Med 172:1011-1017. 172:1011-1017. Med Exp J J Exp Med 172:1011-1017. Behrendt N, Hansen OC, Ploug M, Barkholt V, Koch C (1987) Localization and functional signifi cance of a polymorphic determi- polymorphic a of cance signifi functional and Localization (1987) C Koch V, Barkholt M, Ploug OC, Hansen N, Behrendt Behrendt N, Hansen OC, Ploug M, Barkholt V, Koch C (1987) Localization and functional signifi cance of a polymorphic determi- nant in the third component of human complement. Mol Immunol 24:1097-1103. Immunol Mol complement. human of component third the in nant nant in the third component of human complement. Mol Immunol 24:1097-1103. Koch C, Behrendt N (1986) A novel polymorphism of human complement component C3 detected by means of a monoclonal monoclonal a of means by detected C3 component complement human of polymorphism novel A (1986) N Behrendt C, Koch Koch C, Behrendt N (1986) A novel polymorphism of human complement component C3 detected by means of a monoclonal antibody. Immunogenetics 23:322-325. Immunogenetics antibody. antibody. Immunogenetics 23:322-325.

Cat. no. GAU 013-16 and GAU 017-01 GAU and 013-16 GAU no. Cat. (desArg) C3a/C3a C3a/C3a (desArg) Cat. no. GAU 013-16 and GAU 017-01 Lhotta K, Würzner R, Kronenberg F, Oppermann M, König P (1998) Rapid activation of the complement system by cuprophane cuprophane by system complement the of activation Rapid (1998) P König M, Oppermann F, Kronenberg R, Würzner K, Lhotta Lhotta K, Würzner R, Kronenberg F, Oppermann M, König P (1998) Rapid activation of the complement system by cuprophane depends on complemet component C4. Kidney Int 53:1044-1051. Int Kidney C4. component complemet on depends depends on complemet component C4. Kidney Int 53:1044-1051. Ammon HPT, Ege W, Oppermann M, Göpel W, Eisele S (1995) Improvement in the long-term stability of an amperometric glucose glucose amperometric an of stability long-term the in Improvement (1995) S Eisele W, Göpel M, Oppermann W, Ege HPT, Ammon Ammon HPT, Ege W, Oppermann M, Göpel W, Eisele S (1995) Improvement in the long-term stability of an amperometric glucose sensor system by introducing a cellulose membrane of bacterial origin. Anal Chem 67:466-471. Chem Anal origin. bacterial of membrane cellulose a introducing by system sensor sensor system by introducing a cellulose membrane of bacterial origin. Anal Chem 67:466-471. Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol Immunol 30:935-940. 30:935-940. Immunol Mol molecules. IgG human from separeted Proteins (1993) M Oppermann A, Freywald R, Nezlin Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol Immunol 30:935-940. Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm Pharm 24:119-134. Pharm Imm vivo. in activition complement of Assessment (1992) O Götze U, Höpken M, Oppermann Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm Pharm 24:119-134. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive com- immunosuppressive the of levels plasma Elevated (1991) O Gotze MH, Weber E, Quentin R, Zierz C, Kurts M, Oppermann Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int 40:939-947. Int Kidney failure. renal in Ba fragment plement plement fragment Ba in renal failure. Kidney Int 40:939-947. Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the the through detected as failure renal with patients in activation Complement (1988) O Götze E, Quintin M, Haubitz M, Oppermann Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864. Wochenschr Klin B. Factor and C5, C3, proteins complement the of fragments of quantitation quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864.

Cat. no. HYB 162 162 HYB no. Cat. C4 C4 Cat. no. HYB 162 Jensen ML, Honoré C, Hummelshoj T, Hansen BE, Madsen HO, Garred P (2006) Ficolin-2 recognizes DNA and participates in in participates and DNA recognizes Ficolin-2 (2006) P Garred HO, Madsen BE, Hansen T, Hummelshoj C, Honoré ML, Jensen Jensen ML, Honoré C, Hummelshoj T, Hansen BE, Madsen HO, Garred P (2006) Ficolin-2 recognizes DNA and participates in the clearance of dying host cells. Mol Immunol [Epub ahead of print]. of ahead [Epub Immunol Mol cells. host dying of clearance the the clearance of dying host cells. Mol Immunol [Epub ahead of print]. Frederiksen PD, Thiel S, Larsen CB, Jensenius JC (2005) M-fi colin, an innate immune defence molecule, binds patterns of acetyl acetyl of patterns binds molecule, defence immune innate an colin, M-fi (2005) JC Jensenius CB, Larsen S, Thiel PD, Frederiksen Frederiksen PD, Thiel S, Larsen CB, Jensenius JC (2005) M-fi colin, an innate immune defence molecule, binds patterns of acetyl groups and activates complement. Scand J Immuno 62:462-73. Immuno J Scand complement. activates and groups groups and activates complement. Scand J Immuno 62:462-73. Krarup A, Sorensen UB, Matsushita M, Jensenius JC, Thiel S (2005) Effect of capsulation of opportunistic pathogenic bacteria bacteria pathogenic opportunistic of capsulation of Effect (2005) S Thiel JC, Jensenius M, Matsushita UB, Sorensen A, Krarup Krarup A, Sorensen UB, Matsushita M, Jensenius JC, Thiel S (2005) Effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, L-fi colin, and H-fi colin. Infect Immun 73:1052-60. Immun Infect colin. H-fi and colin, L-fi lectin, mannan-binding molecules recognition pattern the of binding on on binding of the pattern recognition molecules mannan-binding lectin, L-fi colin, and H-fi colin. Infect Immun 73:1052-60. Petersen SV, Thiel S, Jensen L, Vorup-Jensen T, Koch C, Jensenius JC (2000) Control of the classical and the MBL pathway of of pathway MBL the and classical the of Control (2000) JC Jensenius C, Koch T, Vorup-Jensen L, Jensen S, Thiel SV, Petersen Petersen SV, Thiel S, Jensen L, Vorup-Jensen T, Koch C, Jensenius JC (2000) Control of the classical and the MBL pathway of complement activation. Mol Immunol 37:803-11. Immunol Mol activation. complement complement activation. Mol Immunol 37:803-11.

Cat. no. GAU 020-03 and GAU 018-03 GAU and 020-03 GAU no. Cat. H Factor and 010-04 GAU and 008-01 GAU no. Cat. D Factor Factor D Cat. no. GAU 008-01 and GAU 010-04 and Factor H Cat. no. GAU 020-03 and GAU 018-03 Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm Pharm 24:119-134. Pharm Imm vivo. in activition complement of Assessment (1992) O Götze U, Höpken M, Oppermann Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm Pharm 24:119-134. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive com- immunosuppressive the of levels plasma Elevated (1991) O Gotze MH, Weber E, Quentin R, Zierz C, Kurts M, Oppermann Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int 40:939-947. Int Kidney failure. renal in Ba fragment plement plement fragment Ba in renal failure. Kidney Int 40:939-947. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative alternative the of components of Quantitation (1990) O Gotze C, Kurts W, Gottsleben E, Brandt H, Baumgarten M, Oppermann Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190. Methods Immunol J assays. immunosorbent enzyme-linked by (APC) complement of pathway pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.

Cat. no. KIT 029 and HYB 131 HYB and 029 KIT no. Cat. MBL MBL Cat. no. KIT 029 and HYB 131 Please view the MBL reference fl yer, for a complete list of references using KIT 029 or HYB 131. 131. HYB or 029 KIT using references of list complete a for yer, fl reference MBL the view Please Please view the MBL reference fl yer, for a complete list of references using KIT 029 or HYB 131.

Cat. no. HYB 039 039 HYB no. Cat. Properdin Properdin Cat. no. HYB 039 Bathum L, Hansen H, Teisner B, Koch C, Garred P, Rasmussen K, Wang P (2005) Association between combined properdin and and properdin combined between Association (2005) P Wang K, Rasmussen P, Garred C, Koch B, Teisner H, Hansen L, Bathum Bathum L, Hansen H, Teisner B, Koch C, Garred P, Rasmussen K, Wang P (2005) Association between combined properdin and mannose-binding lectin defi ciency and infection with Neisseria meningitidis. Mol Immunol 43:473-479. Immunol Mol meningitidis. Neisseria with infection and ciency defi lectin mannose-binding mannose-binding lectin defi ciency and infection with Neisseria meningitidis. Mol Immunol 43:473-479. Wirthmueller U, Dewald B, Thelen M, Schafer MK, Stover C, Whaley K, North J, Eggleton P, Reid KB, Schwaeble WJ. (1997) (1997) WJ. Schwaeble KB, Reid P, Eggleton J, North K, Whaley C, Stover MK, Schafer M, Thelen B, Dewald U, Wirthmueller Wirthmueller U, Dewald B, Thelen M, Schafer MK, Stover C, Whaley K, North J, Eggleton P, Reid KB, Schwaeble WJ. (1997) Properdin, a positive regulator of complement activation, is released from secondary granules of stimulated peripheral blood blood peripheral stimulated of granules secondary from released is activation, complement of regulator positive a Properdin, Properdin, a positive regulator of complement activation, is released from secondary granules of stimulated peripheral blood neutrophils. J Immunol 158:4444-51. Immunol J neutrophils. neutrophils. J Immunol 158:4444-51. Schwaeble W, Huemer HP, Most J, Dierich MP, Strobel M, Claus C, Reid KB, Ziegler-Heitbrock HW (1994) Expression of proper- of Expression (1994) HW Ziegler-Heitbrock KB, Reid C, Claus M, Strobel MP, Dierich J, Most HP, Huemer W, Schwaeble Schwaeble W, Huemer HP, Most J, Dierich MP, Strobel M, Claus C, Reid KB, Ziegler-Heitbrock HW (1994) Expression of properdin in human monocytes. Eur J Biochem 219:759-64. Biochem J Eur monocytes. human in din din in human monocytes. Eur J Biochem 219:759-64.

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Phone (+45) 4529 0000 4529 (+45) Phone A/S Diagnostics BioPorto BioPorto Diagnostics A/S Phone (+45) 4529 0000 Fax (+45) 4529 0001 4529 (+45) Fax 8 Grusbakken Grusbakken 8 Fax (+45) 4529 0001 E-mail [email protected] E-mail Gentofte DK-2820 DK-2820 Gentofte E-mail [email protected] Tel: +44 (0)23 8048 3000 Web www.bioporto.com Web Denmark Denmark Web www.bioporto.com Web: www.alphalabs.co.uk 6001/052010/e 6001/052010/e