DOCSLIB.ORG

Taxonomy and Systematics of the Australian Sarcophaga S.L. (Diptera: Sarcophagidae) Kelly Ann Meiklejohn University of Wollongong

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Taxonomy and Systematics of the Australian Sarcophaga S.L. (Diptera: Sarcophagidae) Kelly Ann Meiklejohn University of Wollongong University of Wollongong Research Online University of Wollongong Thesis Collection University of Wollongong Thesis Collections 2012 Taxonomy and systematics of the Australian Sarcophaga s.l. (Diptera: Sarcophagidae) Kelly Ann Meiklejohn University of Wollongong Recommended Citation Meiklejohn, Kelly Ann, Taxonomy and systematics of the Australian Sarcophaga s.l. (Diptera: Sarcophagidae), Doctor of Philosophy thesis, School of Biological Sciences, University of Wollongong, 2012. http://ro.uow.edu.au/theses/3729 Research Online is the open access institutional repository for the University of Wollongong. For further information contact the UOW Library: [email protected] Taxonomy and systematics of the Australian Sarcophaga s.l. (Diptera: Sarcophagidae) A thesis submitted in fulfillment of the requirements for the award of the degree Doctor of Philosophy from University of Wollongong by Kelly Ann Meiklejohn BBiotech (Adv, Hons) School of Biological Sciences 2012 Thesis Certification I, Kelly Ann Meiklejohn declare that this thesis, submitted in fulfillment of the requirements for the award of Doctor of Philosophy, in the School of Biological Sciences, University of Wollongong, is wholly my own work unless otherwise referenced or acknowledged. The document has not been submitted for qualifications at any other academic institution. Kelly Ann Meiklejohn 31st of August 2012 ii Table of Contents List of Figures ................................................................................................................................................... vi List of Tables ..................................................................................................................................................... ix List of Appendices ............................................................................................................................................. x List of Abbreviations ....................................................................................................................................... xi Abstract ............................................................................................................................................................ xiv Acknowledgements ........................................................................................................................................ xvi CHAPTER 1: General Introduction ......................................................................................... 1 1.1 Preamble .................................................................................................................... 1 1.2 Distribution, characteristics and life histories of the subfamilies ..................... 1 1.2.1 Miltogramminae ........................................................................................................ 1 1.2.2 Paramacronychiinae ................................................................................................. 2 1.2.3 Sarcophaginae ........................................................................................................... 2 Forensic applications of the Sarcophaginae ................................................ 3 1.3 Species identification of the Sarcophagidae ......................................................... 4 1.3.1 Morphological species identification ..................................................................... 4 1.3.2 Molecular species identification ............................................................................. 5 Protein markers ............................................................................................... 5 DNA markers .................................................................................................. 5 Nuclear DNA ............................................................................................. 6 Mitochondrial DNA ................................................................................. 6 DNA barcoding ................................................................................. 7 1.4 Phylogenetic relationships within the genus Sarcophaga (sensu lato) ................... 8 1.5 Conclusions ............................................................................................................... 9 1.6 Aims ......................................................................................................................... 10 CHAPTER 2: Taxonomy of the Australian Sarcophagidae (Diptera) ................................... 12 2.1 Updates on the distribution and records of Australian Sarcophagidae ........ 12 2.1.1 Introduction ........................................................................................................... 13 2.1.2 Methods .................................................................................................................. 13 2.1.3 Results ..................................................................................................................... 14 New Australian records ............................................................................... 14 Miltogramminae ...................................................................................... 14 Sarcophaginae ......................................................................................... 15 2.1.4 Discussion .............................................................................................................. 19 2.2 Nomenclature and species descriptions ............................................................. 20 2.2.1 Introduction ........................................................................................................... 21 2.2.2 Materials and methods .......................................................................................... 21 iii 2.2.3 Results ..................................................................................................................... 22 Species descriptions ..................................................................................... 22 Sarcophaga (Sarcosolomonia) collessi sp. nov. ................................................. 22 Sarcophaga (Sarcorohdendorfia) clavus sp. nov. .............................................. 25 Nomenclature ............................................................................................... 27 Notes on synonymy: .............................................................................. 27 Notes on rank: ........................................................................................ 29 2.3 A revised key of the Australian Sarcophagidae (Diptera) with special emphasis on Sarcophaga (sensu lato) ....................................................................... 30 2.3.1 Introduction ........................................................................................................... 31 2.3.2 Methods .................................................................................................................. 32 2.3.3 Revised key ............................................................................................................. 33 2.3.4 Species information ............................................................................................... 56 2.3.5 Conclusions ............................................................................................................ 84 2.4 LUCID key for the Australian Sarcophaga (sensu lato) ....................................... 86 CHAPTER 3: Molecular identification of the Australian Sarcophagidae (Diptera) .............. 88 3.1 Comprehensive evaluation of DNA barcoding ................................................ 88 3.1.1 Introduction ........................................................................................................... 89 3.1.2 Materials and methods .......................................................................................... 90 Specimens ...................................................................................................... 90 DNA extraction, amplification and sequencing ...................................... 90 DNA sequence analysis ............................................................................... 91 DNA barcoding analysis ............................................................................. 91 3.1.3 Results and discussion .......................................................................................... 91 Morphological species identifications ....................................................... 91 Inclusion of female specimens ............................................................. 92 Evaluation of DNA Barcoding .................................................................. 93 NJ Tree ..................................................................................................... 93 Percentage divergences .......................................................................... 95 Intraspecific variation ..................................................................... 96 Interspecific variation ..................................................................... 98 Phylogenetic inference ................................................................................ 99 3.1.4 Conclusions ............................................................................................................ 99 3.2 Immature identification using DNA barcoding ............................................. 101 3.2.1 Introduction ........................................................................................................
Load more

Recommended publications
  • Diptera: Calyptratae)
    Systematic Entomology (2020), DOI: 10.1111/syen.12443 Protein-encoding ultraconserved elements provide a new phylogenomic perspective of Oestroidea flies (Diptera: Calyptratae) ELIANA BUENAVENTURA1,2 , MICHAEL W. LLOYD2,3,JUAN MANUEL PERILLALÓPEZ4, VANESSA L. GONZÁLEZ2, ARIANNA THOMAS-CABIANCA5 andTORSTEN DIKOW2 1Museum für Naturkunde, Leibniz Institute for Evolution and Biodiversity Science, Berlin, Germany, 2National Museum of Natural History, Smithsonian Institution, Washington, DC, U.S.A., 3The Jackson Laboratory, Bar Harbor, ME, U.S.A., 4Department of Biological Sciences, Wright State University, Dayton, OH, U.S.A. and 5Department of Environmental Science and Natural Resources, University of Alicante, Alicante, Spain Abstract. The diverse superfamily Oestroidea with more than 15 000 known species includes among others blow flies, flesh flies, bot flies and the diverse tachinid flies. Oestroidea exhibit strikingly divergent morphological and ecological traits, but even with a variety of data sources and inferences there is no consensus on the relationships among major Oestroidea lineages. Phylogenomic inferences derived from targeted enrichment of ultraconserved elements or UCEs have emerged as a promising method for resolving difficult phylogenetic problems at varying timescales. To reconstruct phylogenetic relationships among families of Oestroidea, we obtained UCE loci exclusively derived from the transcribed portion of the genome, making them suitable for larger and more integrative phylogenomic studies using other genomic and transcriptomic resources. We analysed datasets containing 37–2077 UCE loci from 98 representatives of all oestroid families (except Ulurumyiidae and Mystacinobiidae) and seven calyptrate outgroups, with a total concatenated aligned length between 10 and 550 Mb. About 35% of the sampled taxa consisted of museum specimens (2–92 years old), of which 85% resulted in successful UCE enrichment.
    [Show full text]
  • Insect Orders V: Panorpida & Hymenoptera
    Insect Orders V: Panorpida & Hymenoptera • The Panorpida contain 5 orders: the Mecoptera, Siphonaptera, Diptera, Trichoptera and Lepidoptera. • Available evidence clearly indicates that the Lepidoptera and the Trichoptera are sister groups. • The Siphonaptera and Mecoptera are also closely related but it is not clear whether the Siponaptera is the sister group of all of the Mecoptera or a group (Boreidae) within the Mecoptera. If the latter is true, then the Mecoptera is paraphyletic as currently defined. • The Diptera is the sister group of the Siphonaptera + Mecoptera and together make up the Mecopteroids. • The Hymenoptera does not appear to be closely related to any of the other holometabolous orders. Mecoptera (Scorpionflies, hangingflies) • Classification. 600 species worldwide, arranged into 9 families (5 in the US). A very old group, many fossils from the Permian (260 mya) onward. • Structure. Most distinctive feature is the elongated clypeus and labrum that together form a rostrum. The order gets its common name from the gential segment of the male in the family Panorpodiae, which is bulbous and often curved forward above the abdomen, like the sting of a scorpion. Larvae are caterpillar-like or grub- like. • Natural history. Scorpionflies are most common in cool, moist habitats. They get the name “hangingflies” from their habit of hanging upside down on vegetation. Larvae and adult males are mostly predators or scavengers. Adult females are usually scavengers. Larvae and adults in some groups may feed on vegetation. Larvae of most species are terrestrial and caterpillar-like in body form. Larvae of some species are aquatic. In the family Bittacidae males attract females for mating by releasing a sex pheromone and then presenting the female with a nuptial gift.
    [Show full text]
  • Diptera, Empidoidea) of Switzerland
    The Atelestidae and Microphoridae (Diptera, Empidoidea) of Switzerland Autor(en): Merz, Bernhard Objekttyp: Article Zeitschrift: Mitteilungen der Schweizerischen Entomologischen Gesellschaft = Bulletin de la Société Entomologique Suisse = Journal of the Swiss Entomological Society Band (Jahr): 71 (1998) Heft 1-2 PDF erstellt am: 28.09.2021 Persistenter Link: http://doi.org/10.5169/seals-402695 Nutzungsbedingungen Die ETH-Bibliothek ist Anbieterin der digitalisierten Zeitschriften. Sie besitzt keine Urheberrechte an den Inhalten der Zeitschriften. Die Rechte liegen in der Regel bei den Herausgebern. Die auf der Plattform e-periodica veröffentlichten Dokumente stehen für nicht-kommerzielle Zwecke in Lehre und Forschung sowie für die private Nutzung frei zur Verfügung. Einzelne Dateien oder Ausdrucke aus diesem Angebot können zusammen mit diesen Nutzungsbedingungen und den korrekten Herkunftsbezeichnungen weitergegeben werden. Das Veröffentlichen von Bildern in Print- und Online-Publikationen ist nur mit vorheriger Genehmigung der Rechteinhaber erlaubt. Die systematische Speicherung von Teilen des elektronischen Angebots auf anderen Servern bedarf ebenfalls des schriftlichen Einverständnisses der Rechteinhaber. Haftungsausschluss Alle Angaben erfolgen ohne Gewähr für Vollständigkeit oder Richtigkeit. Es wird keine Haftung übernommen für Schäden durch die Verwendung von Informationen aus diesem Online-Angebot oder durch das Fehlen von Informationen. Dies gilt auch für Inhalte Dritter, die über dieses Angebot zugänglich sind. Ein Dienst der ETH-Bibliothek ETH Zürich, Rämistrasse 101, 8092 Zürich, Schweiz, www.library.ethz.ch http://www.e-periodica.ch MITTEILUNGEN DER SCHWEIZERISCHEN ENTOMOLOGISCHEN GESELLSCHAFT BULLETIN DE LA SOCIÉTÉ ENTOMOLOGIQUE SUISSE 71,27-31, 1998 The Atelestidae and Microphoridae (Diptera, Empidoidea) of Switzerland Bernhard Merz Entomological Collection, ETH Zentrum, CH-8092 Zürich, Switzerland The inconspicuous flies of the families Atelestidae and Microphoridae are only rarely collected.
    [Show full text]
  • Diptera, Empidoidea) 263 Doi: 10.3897/Zookeys.365.6070 Research Article Launched to Accelerate Biodiversity Research
    A peer-reviewed open-access journal ZooKeys 365: 263–278 (2013) DNA barcoding of Hybotidae (Diptera, Empidoidea) 263 doi: 10.3897/zookeys.365.6070 RESEARCH ARTICLE www.zookeys.org Launched to accelerate biodiversity research Using DNA barcodes for assessing diversity in the family Hybotidae (Diptera, Empidoidea) Zoltán T. Nagy1, Gontran Sonet1, Jonas Mortelmans2, Camille Vandewynkel3, Patrick Grootaert2 1 Royal Belgian Institute of Natural Sciences, OD Taxonomy and Phylogeny (JEMU), Rue Vautierstraat 29, 1000 Brussels, Belgium 2 Royal Belgian Institute of Natural Sciences, OD Taxonomy and Phylogeny (Ento- mology), Rue Vautierstraat 29, 1000 Brussels, Belgium 3 Laboratoire des Sciences de l’eau et environnement, Faculté des Sciences et Techniques, Avenue Albert Thomas, 23, 87060 Limoges, France Corresponding author: Zoltán T. Nagy ([email protected]) Academic editor: K. Jordaens | Received 7 August 2013 | Accepted 27 November 2013 | Published 30 December 2013 Citation: Nagy ZT, Sonet G, Mortelmans J, Vandewynkel C, Grootaert P (2013) Using DNA barcodes for assessing diversity in the family Hybotidae (Diptera, Empidoidea). In: Nagy ZT, Backeljau T, De Meyer M, Jordaens K (Eds) DNA barcoding: a practical tool for fundamental and applied biodiversity research. ZooKeys 365: 263–278. doi: 10.3897/zookeys.365.6070 Abstract Empidoidea is one of the largest extant lineages of flies, but phylogenetic relationships among species of this group are poorly investigated and global diversity remains scarcely assessed. In this context, one of the most enigmatic empidoid families is Hybotidae. Within the framework of a pilot study, we barcoded 339 specimens of Old World hybotids belonging to 164 species and 22 genera (plus two Empis as outgroups) and attempted to evaluate whether patterns of intra- and interspecific divergences match the current tax- onomy.
    [Show full text]
  • Sphyrna Lewini
    Editora Chefe Profª Drª Antonella Carvalho de Oliveira Assistentes Editoriais Natalia Oliveira Bruno Oliveira Flávia Roberta Barão Bibliotecária Janaina Ramos Projeto Gráfico e Diagramação Natália Sandrini de Azevedo Camila Alves de Cremo Luiza Alves Batista Maria Alice Pinheiro Imagens da Capa 2021 by Atena Editora Shutterstock Copyright © Atena Editora Edição de Arte Copyright do Texto © 2021 Os autores Luiza Alves Batista Copyright da Edição © 2021 Atena Editora Revisão Direitos para esta edição cedidos à Atena Os Autores Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial- NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. Todos os manuscritos foram previamente submetidos à avaliação cega pelos pares, membros do Conselho Editorial desta Editora, tendo sido aprovados para a publicação com base em critérios de neutralidade e imparcialidade acadêmica. A Atena Editora é comprometida em garantir a integridade editorial em todas as etapas do processo de publicação, evitando plágio, dados ou resultados fraudulentos e impedindo que interesses financeiros comprometam os padrões éticos da publicação. Situações suspeitas de má conduta científica serão investigadas sob o mais alto padrão de rigor acadêmico e ético. Conselho Editorial Ciências Humanas e Sociais Aplicadas Prof.
    [Show full text]
  • Protophormia Terraenovae (Robineau-Desvoidy, 1830) (Diptera, Calliphoridae) a New Forensic Indicator to South-Western Europe
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Repositorio Institucional de la Universidad de Alicante Ciencia Forense, 12/2015: 137–152 ISSN: 1575-6793 PROTOPHORMIA TERRAENOVAE (ROBINEAU-DESVOIDY, 1830) (DIPTERA, CALLIPHORIDAE) A NEW FORENSIC INDICATOR TO SOUTH-WESTERN EUROPE Anabel Martínez-Sánchez1 Concepción Magaña2 Martin Toniolo Paola Gobbi Santos Rojo Abstract: Protophormia terraenovae larvae are found frequently on corpses in central and northern Europe but are scarce in the Mediterranean area. We present the first case in the Iberian Peninsula where P. terraenovae was captured during autopsies in Madrid (Spain). In the corpse other nec- rophagous flies were found, Lucilia sericata, Chrysomya albiceps and Sarcopha- ga argyrostoma. To calculate the posmortem interval, the life cycle of P. ter- raenovae was studied at constant temperature, room laboratory and natural fluctuating conditions. The total developmental time was 16.61±0.09 days, 16.75±4.99 days in the two first cases. In natural conditions, developmental time varied between 31.22±0.07 days (average temperature: 15.6oC), 15.58±0.08 days (average temperature: 21.5oC) and 14.9±0.10 days (average temperature: 23.5oC). Forensic importance and the implications of other necrophagous Diptera presence is also discussed. Key words: Calliphoridae, forensic entomology, accumulated degrees days, fluctuating temperatures, competition, postmortem interval, Spain. Resumen: Las larvas de Protophormia terraenovae se encuentran con frecuen- cia asociadas a cadáveres en el centro y norte de Europa pero son raras en el área Mediterránea. Presentamos el primer caso en la Península Ibérica don- 1 Departamento de Ciencias Ambientales/Instituto Universitario CIBIO-Centro Iberoame- ricano de la Biodiversidad.
    [Show full text]
  • Diptera: Empidoidea), with a Redefinition of the Tribe Ocydromiini
    © Copyright Australian Museum, 2000 Records of the Australian Museum (2000) Vol. 52: 161–186. ISSN 0067–1975 Revision of the Genus Apterodromia (Diptera: Empidoidea), With a Redefinition of the Tribe Ocydromiini BRADLEY J. SINCLAIR1 AND JEFFREY M. CUMMING 2 1 Zoologisches Forschungsinstitut und Museum Alexander Koenig, Adenauerallee 160, D-53113 Bonn, Germany [email protected] 2 Systematic Entomology Section, ECORC, Agriculture and Agri-Food Canada, K.W. Neatby Building, C.E.F., Ottawa, ON, Canada K1A 0C6 [email protected] ABSTRACT. The Australian endemic genus Apterodromia Oldroyd (Diptera: Empidoidea) is revised and includes four apterous species (A. evansi Oldroyd, A. minuta n.sp, A. setosa n.sp., and A. tasmanica n.sp.) and eight fully winged species (A. aurea n.sp., A. bickeli n.sp., A. irrorata n.sp., A. monticola n.sp., A. pala n.sp., A. spilota n.sp., A. tonnoiri n.sp., and A. vespertina n.sp.). The male of A. evansi is described and zoogeographic patterns of the genus are discussed. On the basis of wing venation and male terminalia Apterodromia is transferred from the Tachydromiinae to the tribe Ocydromiini (subfamily Ocydromiinae). The Ocydromiini is redefined, two new genera (Neotrichina n.gen. and Leptodromia n.gen.) are described, and all included genera are listed. Keys to major lineages of Australian Empidoidea and Southern Hemisphere genera of Ocydromiini are provided. The following new combinations are listed: Hoplopeza tachydromiaeformis (Bezzi), Leptodromia bimaculata (Bezzi), Neotrichina abdominalis (Collin), N. digna (Collin), N. digressa (Collin), N. distincta (Collin), N. elegans (Bigot), N. fida (Collin), N. indiga (Collin), N.
    [Show full text]
  • A Five-Year Research Program Is Proposed to Expand the Theory of Community Assembly from Its Current Base of Correlative Inferen
    PROJECT SUMMARY A five-year research program is proposed to expand the theory of community assembly from its current base of correlative inferences to one grounded in process-based conclusions derived from controlled field and laboratory experiments. Northern pitcher plants, Sarracenia purpurea, and their community of inquiline arthropods and rotifers, will be used as the model system for the proposed experiments. There are three goals to the proposed research. (1) Inquiline assemblages that colonize pitcher plants will be developed as a model system for understanding community assembly and persistence. (2) Field and laboratory experiments will be used to elucidate causes of inquiline community colonization, assembly, and persistence, and the consequences of inquiline community dynamics for plant leaf allocation patterns, growth, and reproduction, as well as within-plant nutrient cycling. Reciprocal interactions of plant dynamics on inquiline community structure will also be investigated experimentally. (3) Matrix models will be developed to describe reciprocal interactions between inquiline community assembly and persistence, and inquilines’ living host habitats. As an integrated whole, the proposed experiments and models will provide a complete picture of linkages between pitcher-plant inquiline communities and their host plants, at individual leaf and whole-plant scales. This focus on measures of plant performance will fill an apparent lacuna in prior studies of pitcher plant microecosystems, which, with few exceptions, have focused almost exclusively on inquiline population dynamics and interspecific interactions. Plant demography of S. purpurea will be described and modeled for the first time. Complementary, multi-year field and greenhouse experiments will reveal effects of soil and pitcher nutrient composition on leaf allocation, plant growth, and reproduction.
    [Show full text]
  • Diptera: Brachycera: Calyptratae) Inferred from Mitochondrial Genomes
    University of Wollongong Research Online Faculty of Science, Medicine and Health - Papers: part A Faculty of Science, Medicine and Health 1-1-2015 The phylogeny and evolutionary timescale of muscoidea (diptera: brachycera: calyptratae) inferred from mitochondrial genomes Shuangmei Ding China Agricultural University Xuankun Li China Agricultural University Ning Wang China Agricultural University Stephen L. Cameron Queensland University of Technology Meng Mao University of Wollongong, [email protected] See next page for additional authors Follow this and additional works at: https://ro.uow.edu.au/smhpapers Part of the Medicine and Health Sciences Commons, and the Social and Behavioral Sciences Commons Recommended Citation Ding, Shuangmei; Li, Xuankun; Wang, Ning; Cameron, Stephen L.; Mao, Meng; Wang, Yuyu; Xi, Yuqiang; and Yang, Ding, "The phylogeny and evolutionary timescale of muscoidea (diptera: brachycera: calyptratae) inferred from mitochondrial genomes" (2015). Faculty of Science, Medicine and Health - Papers: part A. 3178. https://ro.uow.edu.au/smhpapers/3178 Research Online is the open access institutional repository for the University of Wollongong. For further information contact the UOW Library: [email protected] The phylogeny and evolutionary timescale of muscoidea (diptera: brachycera: calyptratae) inferred from mitochondrial genomes Abstract Muscoidea is a significant dipteran clade that includes house flies (Family Muscidae), latrine flies (F. Fannidae), dung flies (F. Scathophagidae) and root maggot flies (F. Anthomyiidae). It is comprised of approximately 7000 described species. The monophyly of the Muscoidea and the precise relationships of muscoids to the closest superfamily the Oestroidea (blow flies, flesh flies etc)e ar both unresolved. Until now mitochondrial (mt) genomes were available for only two of the four muscoid families precluding a thorough test of phylogenetic relationships using this data source.
    [Show full text]
  • Serine Protease Activities in Oxysarcodexia Thornax (Walker) (Diptera: Sarcophagidae) First Instar Larva
    504 Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 103(5): 504-506, August 2008 Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva Patrícia Cuervo, Camila Mesquita-Rodrigues1, Claudia Masini d’Avila Levy1, Constança Britto1, Fabiano Araújo Pires1, Rodrigo Gredilha2, Carlos Roberto Alves1, Jose Batista de Jesus1/3/+ Laboratório de Pesquisas em Leishmanioses 1Laboratório de Biologia Molecular e Doenças Endêmicas 2Laboratório de Diptera, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil 3Departamento de Ciências Naturais, Universidade Federal de São João del Rei, São João del Rei, MG, Brasil We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotrypsin-like serine proteases. Keywords: Oxysarcodexia thornax - Sarcophagidae - chymotrypsin-like serine proteases The genus Oxysarcodexia belongs to the Sarcophagi- Despite the existence of information about morphol- dae family (flesh flies) whose major biological feature ogy, taxonomy and ecology of this genus, as far as we is the ovo-larvipary (Pape 1996).
    [Show full text]
  • Species-Specific Behavioral Differences in Tsetse Fly Genital
    Chapter 15 Species-Specific Behavioral Differences in Tsetse Fly Genital Morphology and Probable Cryptic Female Choice R.D. Briceño and W.G. Eberhard Abstract A long-standing mystery in morphological evolution is why male geni- talia tend to diverge more rapidly than other structures. One possible explana- tion of this trend is that male genitalia function as “internal courtship devices,” and are under sexual selection by cryptic female choice (CFC) to induce female responses that improve the male’s chances of fathering her offspring. Males of closely related species, which have species-specific genital structures, are thought to provide divergent stimulation. Testing this hypothesis has been difficult; the pre- sumed genital courtship behavior is hidden from view inside the female; appropri- ate experimental manipulations of male and female genitalia are often technically difficult and seldom performed; and most studies of how the male’s genitalia inter- act with those of the female are limited to a single species in a given group, thus limiting opportunities for comparisons of species-specific structures. In this chap- ter, we summarize data from morphological, behavioral, and experimental studies of six species in the tsetse fly genus Glossina, including new X-ray recordings that allowed visualization of events inside the female during real time. Species-specific male genital structures perform dramatic, stereotyped, rhythmic movements, some on the external surface of the female’s abdomen and others within her reproduc- tive tract. Counting conservatively, a female Glossina may sense stimuli from the male’s genitalia at up to 8 sites on her body during some stages of copulation.
    [Show full text]
  • ARTHROPODA Subphylum Hexapoda Protura, Springtails, Diplura, and Insects
    NINE Phylum ARTHROPODA SUBPHYLUM HEXAPODA Protura, springtails, Diplura, and insects ROD P. MACFARLANE, PETER A. MADDISON, IAN G. ANDREW, JOCELYN A. BERRY, PETER M. JOHNS, ROBERT J. B. HOARE, MARIE-CLAUDE LARIVIÈRE, PENELOPE GREENSLADE, ROSA C. HENDERSON, COURTenaY N. SMITHERS, RicarDO L. PALMA, JOHN B. WARD, ROBERT L. C. PILGRIM, DaVID R. TOWNS, IAN McLELLAN, DAVID A. J. TEULON, TERRY R. HITCHINGS, VICTOR F. EASTOP, NICHOLAS A. MARTIN, MURRAY J. FLETCHER, MARLON A. W. STUFKENS, PAMELA J. DALE, Daniel BURCKHARDT, THOMAS R. BUCKLEY, STEVEN A. TREWICK defining feature of the Hexapoda, as the name suggests, is six legs. Also, the body comprises a head, thorax, and abdomen. The number A of abdominal segments varies, however; there are only six in the Collembola (springtails), 9–12 in the Protura, and 10 in the Diplura, whereas in all other hexapods there are strictly 11. Insects are now regarded as comprising only those hexapods with 11 abdominal segments. Whereas crustaceans are the dominant group of arthropods in the sea, hexapods prevail on land, in numbers and biomass. Altogether, the Hexapoda constitutes the most diverse group of animals – the estimated number of described species worldwide is just over 900,000, with the beetles (order Coleoptera) comprising more than a third of these. Today, the Hexapoda is considered to contain four classes – the Insecta, and the Protura, Collembola, and Diplura. The latter three classes were formerly allied with the insect orders Archaeognatha (jumping bristletails) and Thysanura (silverfish) as the insect subclass Apterygota (‘wingless’). The Apterygota is now regarded as an artificial assemblage (Bitsch & Bitsch 2000).
    [Show full text]