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MAT KADI TORA TUTTI USTUTTO 20180148498A1 ULTIMAHORA EN LITHUANIA ( 19) United States (12 ) Patent Application Publication ( 10) Pub . No. : US 2018 /0148498 A1 Kozel et al. (43 ) Pub . Date : May 31 , 2018 ( 54 ) FUNGAL DETECTION USING MANNAN Publication Classification EPITOPE (51 ) Int. Cl. @(71 ) Applicant: BOARD OF REGENTS OF THE COZK 16 / 14 (2006 .01 ) NEVADA SYSTEM OF HIGHER GOIN 33 /569 ( 2006 . 01) EDUCTION , ON BEHALF OF THE CO7K 16 / 44 ( 2006 . 01 ) UNIVERSITY OF NEVADA , RENO , (52 ) U . S . CI. NV (US ) CPC . .. .. CO7K 16 / 14 ( 2013 .01 ) ; GOIN 33 /56961 ( 2013 .01 ) ; COZK 2317/ 622 (2013 . 01 ) ; COOK @(72 ) Inventors: Thomas R . Kozel , Reno , NV (US ) ; 2317 /33 (2013 . 01 ) ; CO7K 2317/ 92 ( 2013 .01 ) ; Breeana HUBBARD , Pullman , WA (US ) ; Amanda CO7K 16 /44 ( 2013 .01 ) BURNHAM -MARUSICH , Reno , NV (US ) ( 57 ) ABSTRACT ( 21) Appl . No. : 15 /567 , 547 (22 ) PCT Filed : Apr. 23 , 2016 Non - invasive methods are provided herein for diagnosing samples as including a fungus , including fungal infection or ( 86 ) PCT No. : PCT/ US16 /29085 contamination , with specific monoclonal antibodies capable $ 371 ( c) ( 1 ), of detecting molecules associated with fungi in the sample , ( 2 ) Date : Oct. 18 , 2017 such as a biological or environmental sample . These mol ecules can be identified using various methods, including Related U . S . Application Data but not limited to antibody based methods , such as an ( 60 ) Provisional application No. 62 /151 , 865, filed on Apr . enzyme- linked immunosorbant assay (ELISA ) , or a lateral 23 , 2015 . flow immunoassay . Limit of detection (nam ) Vucor Aspergillus 540 Ugariun Reciprocaltiter . Candida 16:55555555555555555555555555555555555555 öö Mucor Aspergillus Fusarum Candida Patent Application Publication May 31 , 2018 Sheet10f8 US 2018/ 0148498Al ????????????????????????????????????????????????????????????????????????????? ??_ ??? f ' 9 ? ? ? ????? ?????$ ? / 28; & ? ? {QK ? Reciprocaltiter é 4 ????????????????????????????????????????????????????????????????????????????????????????????????????????2 é _é AHATARCH Aspergillus Fusarium Candida FIG1 Patent Application Publication May 31 , 2018 Sheet 2 of 8 US 2018 / 0148498 A1 mAb 2DA6 DNA Sequence Light chain : DNA sequence ( 384 bp ) (SEQ ID NO : 1 ) Leader sequence ATGGATTTTCAGGTGCAGATTTTCAGCTTCCTGCTAATCAGTGCCTCAGTCATTATGTCCAGAGGA C FR1 AAATTGTTCTCACCCAGTCTCCAGCAATCATGTCTGCATCTCCAGGGCAGAAAGTCACCATAACCTG CDR1 FR2 ? AGTGTCAGCTCAAACATACATTTCATGCAC TGGTACCAGCAGAAGTTAGGATTc?ccccCAAACTC CDR2 TGGATTTAT GACACATCCAAACTGACTCCT GGAGTCCCTGCTCGCTTCAGTGGCAGTGGGTCTGGGA FR3 CCTCTTACTCTCTCACAATCAGCAGCATGGAGGCTGAAGATGCTGCCTCTTATTTCTGC CATCAGTG CDR3 FR4 GAGTAGTCACCCACATACG TTCGGATCGGGGACCAAGCTGGAAATAAAA Heavy chain : DNA sequence ( 408 bp ) (SEQ ID NO : 2 ) Leader sequence ATGGATTGGACCTGGGTCTTTCTCTTCCTCCTGTCAGTAACTGCAGGTGTCCACTCC CAGGTTCAGC FR1 TGCAGCAGTCTGGAGCTGAGCTGATGAAGCCTGGGGCCTCAGTGAGGCTTTCCTGCAAGACTACTGG CDR1 FR2 CTACACATTCACT GGCAACTGGATTGAG TGGATAAAGCAGAGGCCTGGACATGGCCTTGAGTGGATT CDR2 GGA GAGATCTTACCTGGAAATAGTCGGACTAATTATAATGAGAAGTTTAAGGGC AAGGCCACTTTCA FR3 CTGCAGATACATCCTCCAACACAGCCTACATGCAACTCAGCAGCCTGACACCTGAAGACTCTGCCAT CDR3 FR4 CTATTTCTGTGCAAGA GTGACTGGGACGTCCTTTGACTACT GGGGCCAAGGCACCACTCTCACAGTC TCCTCT FIG . 2A Patent Application Publication May 31 , 2018 Sheet 3 of 8 US 2018 / 0148498 A1 mAb 2DA6 Amino Acid Sequence Light chain : Amino acids sequence ( 128 AA ) (SEQ ID NO : 3 ) 1 Leader sequence FR1 1 CDR1 FR2 MDFQVQIFSFLLISASVIMSRG QIVLTQSPAIMSASPGQKVTITC SVSSNIHFMH WYQQKLGFSPK LCDR21 FR3 1 CDR3 I FR4 1 LWIY DTSKLTP GVPARFSGSGSGTSYSLTISSMEAEDAASYFC HQWSSHPHT FGSGTKLEIK Heavy chain : Amino acids sequence ( 136 AA ) (SEQ ID NO : 4 ) 1 Leader sequence | FR1 | CDR11 FR2 MDWTWVFLFLLSVTAGVHS QVQLQQSGAELMKPGASVRLSCKTTGYTFT GNWIE WIKQRPGHGLEWI CDR2 FR3 | CDR3 1 FR4 G EILPGNSRTNYNEKFKG KATFTADTSSNTAYMOLSSLTPEDSAIYFCAR VTGTSFDY WGQGTTLT VSS FIG . 2B Patent Application Publication May 31 , 2018 Sheet 4 of 8 US 2018 / 0148498 A1 Clone 11C16 Light chain (SEQ ID NO : 5 ) < < - - - - - - -- -— —- - - - —- —- - - - -- -- - - - —- —- - - - - - - - - - - FR1 - IMGT -- - - - - -- - - —- -— —- - -- - - gagct g t g gactcaqtcgccctct . acatct Ccgcccta ggagcctcggccaag von monen mensen montere mom mom moet moet saam met meer nou wo mom mom moet ons >> CDR1 - IMGT < - - - - - - - .. .. .. 10tca 1 cda . accta cacca t t www .. .. FR2 _ IMGT - - - - - wwwwwwwwwww - CDR t a Bacaace gagga t ata 03 c aacttaa ga . 2 - IMGT - - - - - - - - - - - - - - - - - - - - - - - - |- - - - - - - - - - - - - - - - - - - - . .. .. ggaacctaggaacctacaccaaagagaccggtgtccct caccaa g a cct . gatcgcttctcgggctccago 1 - - - FR3 - IMGT - - - - - I I - . .. totctggggctgaccgctacttgatcatctccagcgtccaggttgatgacgaggcc tact tcatctcc rt a - - - -- - |- - - - - - - > CDR3 - IMGT o IOctacta at rt ttattc ggcggagagaccc 0) c rt g Heavy chain (SEQ ID NO : 6 ) V< - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - FR1 - IMGT - - - - - - - - - - . - - CDR1 - IMGT < - - - - - . tca 0 caaagcct tagattctccctcgal LCCCCCCC . aqtgactactaaataaac - - - - - - - - - - - - - - - FR2 - IMGT - - - - - - - - - - - - - - - - - - - > CDR 0 g tccagaga ID gaaccattaqta ctaat . .2 -. IMGT. ggtagcacatactacacgagctgggcgaaa- - - - - - - - - - - - - - - -- - - - - .- -. ggccgattcaccatctccaaa- - - 1 - - -- - FR3 _ IMGT - acct g a CC . ac 10 C a a g a a 10 c gagga a C - - - - - T- - - - > CDR3 - IMGT D acctattID anel 0st Cca agaacatatatt gat atttct gggc FIG . 3A Patent Application Publication May 31 , 2018 Sheet 5 of 8 US 2018 / 0148498 A1 Clone 9M12 Light Chain (SEQ ID NO : 7 ) V< .- .. .. -. -. -. -. - - - - - - - - - - - - - - - - - - - FR1 - IMGT - - - - - - .. gaga . cagctggtggagtccgggggt . cgcctggtcacgcctgggacacccctgacaa g a * * * * na nova con una nuova au -- > CDR1 - IMGT - - -- - - - - - ctcgcctgcacagcctctggattctccctct ca acct CCTC . .. .. .. agtagccacgacatgatctc I | | - - - - - - FR2 - IMGT I | - L CDR tgggtccgccaggctccaggggagggactggaatacatcggatacattactgctggtg X rt catta . < - - - - - - - - T - - - - - - - - - - - - - - - - - - - - - - - - - - v at tutututututute - 2 - IMGT Hitta tutta . ggtagcccatactacgcgagctgggcaaaa- - . ggccgattcaccatctccaga- wwwwwwww 23 - IMGT 1 ** * * * * www ww www www www * * * * * * www wwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwwww accacctcgacc a . acggtggatctgaaaatggccagtctgacaaccgagacacggccaa c t ac - - - - - - - - > cgtatttctg Sequence cut short - no CDR3 identified Heavy chain (SEQ ID NO : 8 ) < - - - - - - - - - - - -- - -- - - - - - - - - - - - - - - - FR1 - IMGT - - - . ccctgaca -- - -* -* --* -* we wou wou vou mouww - - o- ou o noso sou wou now. - - -- > CDR1 - IMGT < - - - -ou nou mode ctcgcctgcacagcctctggattctccctct a CCCCC . .. .. agtagocacgacatgatocca t 1 I | - | | CDR tgggtccgccaggctccaggggagggactggaatacatcggatacattactgctggtUT g ta > atta . 2 - IMGT - |- 1- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - . ggtagcccatactacgcgagctgggcaaaaCccatac g c aaa . .. ggccgattcaccatctccagatca CCat ct 1 -- - - - - - - - FR3 - IMGT - - - - - - - -- - * * * * www www it with a www * * w ith i with Mr + w w w w www www t 0 c tcgacca CC . acggtggatctgaaaatggccagtctgacaaccgaggacacggccg g a a ??? a d a c - - - - - - - - - - - > CDR3 - IMGT acgtatttctgtggcagaggtgcttattctggttttggttttgacatctggggcccaggc FIG . 3B Patent Application Publication May 31 , 2018 Sheet 6 of 8 US 2018 / 0148498 A1 LODSUMMANRANNANAMAMAR (nomo Wild type 38 Mon 0 . 32 Reciprocaltiter ãogo Reciprocaltiter wwwwwwwwwwwwwwwwwwwwwwwwwww wa! WWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWWW ???the Mnn2 FIG . 4 Reciprocaltiter oano ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? *hint theother WT-periodate ??y??????** WT-mock M???periodate -I???K Mnn2SET ???late -periodate WT -Proteinase k* WT-mock Mnn2Proteinasek -mock ProteinaseWITH k FIG . 5 Patent Application Publication May 31 , 2018 Sheet 7 of 8 US 2018 / 0148498 A1 FIG . 6 ** * zoKuomiz Basidiorrycota ????????????????????? ?????????????????????????????. ??? wwindidriuwwwwwwwwrive viriki * * * * * NorversitA nnicolai es in wwwwwwwww som ö Reciprocaltiter ö mmmmmmmmmmmmmmmmmmmmmmmmmmmmmm ö nnnnnnnnnwwwwwwwwwwwwwwwwwwwwww Sunwinnnn Rhizopusoryzae Mucom als Deu ameffei Batrachachytrumderdrobat dis sn0000000 jou Malassezia steuSchizosaccharomycesPseudogmascusPneumocystis furfur carina MicrosporumpomkedestructansEpidemiophyton Timagophytes Aspergilluscanis floccosumPencilium fumigatus BotrytisStromatinia Scedosporium cinerea Fusariumcepiola Maghaporthaaposem solaniSaccharomyces Cardida Onze albicans cerevisiae Patent Application Publication May 31 , 2018 Sheet 8 of 8 US 2018 / 0148498 A1 human and veterinary dematophytes Fungi producing combat- induced Wavefga } { { ??17 { ? Negativecontrol07007 $£N # Negativecontrol MaudouintiM.cans 1 Semudabauau Toubra 7?. tonsurans Iviola Rhizopus001111 „ Shradsy Control ???????????????? FIG . 7 Blue stain fungus in pinus Allium white rot Grosmannia clavigera Stromatinta cepivora tittar ETT: : tart 1 e work CE a SA 1 th1528500 tra seasedE Control Test FIG . 8 US 2018 /0148498 A1 May 31 , 2018 FUNGAL DETECTION USING MANNAN fungi , such as from fungal infection or contamination , in a EPITOPE sample or subject . The present invention satisfies this need . CROSS -REFERENCE TO RELATED SUMMARY OF THE INVENTION APPLICATIONS [0007 ] As described herein , the present invention relates 10001 ] The present application claims priority under 35 to compositions and methods for detecting a fungus in a U . S . C . $ 119 ( e ) to U . S .
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    Seventeenth Australasian Weeds Conference In vitro assessment of Stromatinia cepivora as a potential biological control agent for angled onion (Allium triquetrum) in Victoria, Australia Parsa Tehranchian1, A.C. Lawrie1 and Robin Adair2 1 RMIT University, Bundoora West Campus, PO Box 71, Bundoora, Victoria 3083, Australia 2 Department of Primary Industries, Frankston Centre, PO Box 48, Frankston, Victoria 3199, Australia Corresponding author: [email protected] Summary Angled onion (Allium triquetrum L.) is a in bushland, and there is no current selective control noxious weed in Australia and is difficult to control, method. especially in natural habitats. The genetic diversity Stromatinia cepivora causes a serious, fatal white of 11 provenances was assessed by RAPD-PCR and rot of cultivated Allium species, identified easily by was relatively small, making it a suitable biological the small black sclerotia produced on white hyphae control target. Pathogenicity trials subsequently used (Agrios 2005, Alexopoulos and Mims 1979). It is an five typical provenances in axenic conditions to test the imperfect fungus (Alexopoulos and Mims 1979) that potential of biological control by Stromatinia cepivora produces many tiny sclerotia (0.25–0.6 mm diameter) (Berk.) Whetzel (Sclerotium cepivorum Berk.), a fatal (Kay and Stewart 1994). The sclerotia can survive soil-borne pathogenic fungus of cultivated Allium dormant in soil for up to 40 years in the absence of species. Pathogenicity and virulence of the fungus host plants without losing their viability (Coley-Smith were assessed using a S. cepivora isolate supplied by 1979, Coley-Smith et al. 1990) and are transferred by DPI Knoxfield. Micropropagation was necessary to soil and water movement.
  • Bab 1 Pendahuluan

    Bab 1 Pendahuluan

    1 2 3 KATA PENGANTAR Buku Mikologi disusun dalam rangka melengkapi khasanah keilmuan bidang biologi khususnya Mikrobiologi. Ditujukan untuk mahasiswa baik program sarjana maupun peminat ilmu dasar mengenai jamur atau fungi. Dalam proses belajar mengajar di Perguruan Tinggi diperlukan adanya buku acuan dan bahan ajar sebagai bahan untuk dikembangkan baik oleh pengajar maupun oleh mahasiswa sehingga proses belajar akan tercapai sesuai dengan garis besar perkuliahan. Buku ini masih jauh dari sempurna, oleh karena itu penulis mohon maaf atas kekurangan-kekurangannya, namun demikian semoga buku ini bermanfaat bagi yang menggunakannya. Dengan tersusunnya buku ini, penulis menyampaikan terima kasih yang sebesar-besarnya atas bantuan dari semua pihak. Semoga buku ini dapat bermanfaat secara maksimal untuk seluruh pembaca. Terimakasih. Bandung, Januari 2020 Penulis 4 DAFTAR ISI Kata Pengantar .................................................................. iii Daftar Isi ................................................................... iv 1. Pendahuluan .............................................................. 1 A. Lata Belakang ........................................................ 1 B. Definisi dan Pengertian Umum Jamur ................... 2 2. Sifat-Sifat Umum Jamur........................................... 4 A. Morfologi dan Anatomi Jamur .............................. 4 1. Hifa dan Miselium ........................................... 4 2. Dinding Hifa .................................................... 6 3. Membran Hifa .................................................
  • Clinical Policy: Topical Agents: Anti-Fungals Reference Number: OH.PHAR.PPA.90 Effective Date: 01/01/2020 Revision Log Last Review Date: Line of Business: Medicaid

    Clinical Policy: Topical Agents: Anti-Fungals Reference Number: OH.PHAR.PPA.90 Effective Date: 01/01/2020 Revision Log Last Review Date: Line of Business: Medicaid

    Clinical Policy: Topical Agents: Anti-Fungals Reference Number: OH.PHAR.PPA.90 Effective Date: 01/01/2020 Revision Log Last Review Date: Line of Business: Medicaid See Important Reminder at the end of this policy for important regulatory and legal information. Description NO PA REQUIRED “PREFERRED” PA REQUIRED “NON-PREFERRED” CICLOPIROX cream, gel, topical suspension, shampoo CICLOPIROX kit (generic of CNL® Nail lacquer kit) (generic of Loprox®) ERTACZO® (sertaconazole) CICLOPIROX solution (generic of Penlac®) EXELDERM® (sulconazole) CLOTRIMAZOLE (generic of Lotrimin®) JUBLIA® solution (efinaconazole) CLOTRIMAZOLE/BETAMETHASONE (generic of KERYDIN® solution (tavaborole) Lotrisone®) KETOCONAZOLE foam(generic of Extina®) ECONAZOLE (generic of Spectazole®) LUZU® (luliconazole) KETOCONAZOLE cream & shampoo (generic of Kuric®, MENTAX® (butenafine) Nizoral®) NAFTIFINE CREAM MICONAZOLE NAFTIN® GEL (naftifine) NYSTATIN OXICONAZOLE (generic of OXISTAT®) NYSTATIN/TRIAMCINOLONE PEDIADERM AF® cream (nystatin) TERBINAFINE (generic of Lamisil®) VUSION® ointment (miconazole/zinc) TOLNAFTATE (generic of Tinactin®) FDA approved indication(s) Ciclopirox is indicated for: • Topical treatment of mild to moderate onychomycosis of fingernails and toenails without lunula involvement, due to Trichophyton rubrum in immunocompetent patients (Penlac®, Ciclodan Nail Lacquer®) • Topical treatment of seborrheic dermatitis of the scalp (Loprox®) • Topical treatment of tinea corporis, tinea cruris, or tinea pedis (Epidermophyton floccosum; Microsporum canis; Trichophyton
  • Plant Biosecurity Report 2019-2020

    Plant Biosecurity Report 2019-2020

    Plant Biosecurity Report 2019-2020 Bhutan Agriculture and Food Regulatory Authority (BAFRA) Ministry of Agriculture and Forests (MoAF) Thimphu i Introduction The plant biosecurity is gaining significance with expansion of global trade of agricultural commodities and transboundary movement of plant genetic resources. The catastrophic impact through intrusion of pest and disease like historical Irish famine caused by potato late blight introduced from Central America and Golden nematode of potato introduced to India in 1960s from the UK furnished vivid evident that introduction and establishment of quarantine pest affects the crop production and economy of the country (Khetarpal, R.K. and Gupta, K. 2007). Currently, International Plant Protection Convention (IPPC) requires all contracting parties to establish National Plant Protection Organization (NPPO) and develop international cooperation in prevention of introduction and spread of regulated pest. In Australia, several area of major biosecurity concerns are new race of wheat stem rust, loss of pollination European honey bee services from parasite Varroa mite and disease, incursion of exotic fruit fly. Wheat stem rust epidemic in 1973 in Australia caused about $200-300 million and expected yield loss of more than 70 percent. Wheat stem rust, Ug99 is now only found in African countries and Middle East, although Ug99 is under control in Australia for more than 30 years, there is a potential threat to wheat industry as eradication and containment become difficult due to high mobility of the disease. The disease spread to larger distance by wind, movement of goods and people. Loss of European honey bees will severely impact fruits and vegetables as loss of pollination services can impact economy of the country which has annual contribution of $4-6 billion.
  • Davis Overview of Fungi and Diseases 2014

    Davis Overview of Fungi and Diseases 2014

    JHH ID Tutorials Dr Josh Davis December 2014 MYCOLOGY OVERVIEW 1. OVERVIEW. It is estimated that there are 1.5 million extant species of fungi on Earth, of which 60,000 have been described/named; of these only approximately 400 species have ever been described to cause disease in humans and only approximately 20 do so with any frequency. Many others are plant pathogens or symbionts. At least 13,500 fungal species form lichens, symbiotic partnerships between fungi (usually ascomycota) and photosynthetic microbes (eg. algae, cyanobacteria). 2. CLASSIFICATION. There are several confusing/overlapping classifications systems for fungi. 2.1 Biological Kingdom FUNGI; Phyla: 2.1.1 Phylum Zygomycota – Agents of zygomycosis, “mucormycosis”. Most primitive fungi. Broad, ribbon-like hyphae, no septae. Generally grow fast on agar (“lid-lifters”). 2.1.1.1 Order Mucorales – eg. Rhizopus, Rhizomucor, Mucor, Saskanaea, Cuninghamella 2.1.1.2 Order Entomophthorales – Basidiobolus, Canidiobolus 2.1.2 Phylum Basidiomycota – Mushrooms, jelly fungi, smuts, rusts, stinkhorns . and the teleomorph of cryptococcus! 2.1.3 Phylum Ascomycota – e.g. Pseudoallescheria, Curvularia, Saccharomyces. 2.1.4 Phylum Deuteromycota, or Fungi Imperfecti. Not a true phylum. Contains asexual (“imperfect”) forms of fungi (anamorphs), most of which have not had a sexual form described. Most human pathogens are in this group – eg: Aspergillus, Candida, Cryptococcus, Scedosporium, Alternaria, Trichophyton, Cladosporium etc. etc. 1. Sporotrix at 37 and 25 degrees; 2. Aspergillus fumigatus, niger, terreus, flavus (L to R); 3. Candida albicans 2.2 Morphological 2.2.1 Broad classification - Yeasts, Moulds and Dimorphic Fungi 2.2.1.1 Yeasts are single-celled organisms which reproduce by budding and grow as smooth colonies on agar.