Humidicutis Dictiocephala Fungal Planet Description Sheets 333

332 Persoonia – Volume 38, 2017

Humidicutis dictiocephala Fungal Planet description sheets 333

Fungal Planet 600 – 20 June 2017 Humidicutis dictiocephala A. Barili, C.W. Barnes & Ordoñez, sp. nov.

Etymology. Name reflects the morphology of the pileus. Notes — According to the description of Young (1999), Hu- midicutis dictiocephala belongs to the subgenus Humidicutis. Classification — Hygrophoraceae, Agaricales, Agaricomy- However, the combination of observed characters does not lead cetes. to a species identification. Horak’s (1990) key for Humidicutis Basidiomata stipitate, pileus conical, umbonate, 20 mm high, indicates H. conspicua as the closest species, but it differs 22 mm diam, orange, velvety-rough surface, radially fibrillose, from H. dictiocephala by having a fibrillose, dry pileal surface, margin rimose. No distinct odour or taste. Lamellae emarginate, margin whole, lamellae not bright orange but whitish in colour, thick, waxy, pale orange to whitish, anastomosed, subdistant, and larger basidia and basidiospores. The description of Lodge with lamellae present, smooth margin. Stipe central, 80 × 5 mm, et al. (2014) places H. dictiocephala within the genus Humidi- yellowish at the apex, pale orange at the base, hollow, smooth, cutis, differing from the closely related Porpolomopsis by the dry. Pileipellis as cutis with cylindrical parallel hyphae, clamp short hyphae of the lamellar trama and by the adnate lamellae. connections absent. Lamellar trama irregular to subregular. The complete ITS sequence of 571 bp of the H. dictiocephala Basidia 36.5–54 × 6–10.5 µm, elongate, clavate, tetrasporic, holotype was used for the BLASTn search. Phylogenetic ana toruloid clamp connections at the base, sterigmata 5.5–9 µm lysis was done using representative sequences from the top long. Basidiospores 6.5–9 × 4.5–6 µm, ellipsoid, subcylindrical, BLASTn hit species. The results gave the two highest scores smooth with subtle wall, hyaline, non-amyloid, non-dextrinoid, as Humidicutis sp. from Belize (GenBank KF291110), and from not metachromatic, without germ pore, apiculate. Puerto Rico (GenBank KF291150) reported by Lodge et al. Habit — Solitary, on the ground, high montane forest. (2014). Following the Humidicutis sp. in the BLASTn search results were 11 sequences of Hygrocybe auratocephalus, but Typus. Ecuador, Zamora Chinchipe province, Yacuri National Park, alt. 3 234 m, May 2015, C. Vivanco (holotype QCAM6000, ITS and LSU only two representative sequences were used for the sequence sequences GenBank KY689661 and KY780120, MycoBank MB820098, alignment. Finally, we included sequences from two uncultured TreeBASE Submission ID 20678). fungal clones, both ectomycorrhizal, and two sequences of Humidicutis marginata for the outgroup.

The phylogenetic tree was constructed using the Maximum Likelihood plugin PhyML in Geneious R9 (http://www.geneious. com; Kearse et al. 2012), and the substitution model determined by jModelTest (Posada 2008) according to Corrected Akaike Information Criterion (AICc). Humidicutis marginata (GenBank KF291144 and DQ490625) was chosen as outgroup. Boot- strap support values ≥ 50 % are given above branches. The phylogenetic position of H. dictiocephala is indicated in bold. The species name is followed by the GenBank ID, and where known, the country of origin indicated as: USA = United States; Colour illustrations. Ecuador, Yacuri National Park; basidiocarp, lamellar ECU = Ecuador; PRI = Puerto Rico; BLZ = Belize; MDG = trama, basidia and basidiospores. Scale bars = 10 µm. Madagascar; AUS = Australia.

Alessio Barili & Maria E. Ordoñez, Escuela de Ciencias Biológicas, Pontiﬁcia Universidad Católica del Ecuador, Av. 12 de octubre 1076 y Roca, Quito, Ecuador; e-mail: [email protected] & [email protected] Charles W. Barnes, Departamento Nacional de Protección Vegetal, Estación Experimental Santa Catalina, Instituto Nacional de Investigaciones Agropecuarias, Panamericana Sur Km. 1 vía Tambillo, Cantón Mejía, Provincia de Pichincha, Quito, Ecuador; e-mail: [email protected]