plants Article Differential Evolutionary History in Visual and Olfactory Floral Cues of the Bee-Pollinated Genus Campanula (Campanulaceae) Paulo Milet-Pinheiro 1,*,† , Pablo Sandro Carvalho Santos 1, Samuel Prieto-Benítez 2,3, Manfred Ayasse 1 and Stefan Dötterl 4 1 Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Albert-Einstein Allee, 89081 Ulm, Germany;
[email protected] (P.S.C.S.);
[email protected] (M.A.) 2 Departamento de Biología y Geología, Física y Química Inorgánica, Universidad Rey Juan Carlos-ESCET, C/Tulipán, s/n, Móstoles, 28933 Madrid, Spain;
[email protected] 3 Ecotoxicology of Air Pollution Group, Environmental Department, CIEMAT, Avda. Complutense, 40, 28040 Madrid, Spain 4 Department of Biosciences, Paris-Lodron-University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria;
[email protected] * Correspondence:
[email protected] † Present address: Universidade de Pernambuco, Campus Petrolina, Rodovia BR 203, KM 2, s/n, Petrolina 56328-900, Brazil. Abstract: Visual and olfactory floral signals play key roles in plant-pollinator interactions. In recent decades, studies investigating the evolution of either of these signals have increased considerably. However, there are large gaps in our understanding of whether or not these two cue modalities evolve in a concerted manner. Here, we characterized the visual (i.e., color) and olfactory (scent) floral cues in bee-pollinated Campanula species by spectrophotometric and chemical methods, respectively, with Citation: Milet-Pinheiro, P.; Santos, the aim of tracing their evolutionary paths. We found a species-specific pattern in color reflectance P.S.C.; Prieto-Benítez, S.; Ayasse, M.; and scent chemistry.