MMP19, Matrix 19 polyclonal antibody

roteins of the (MMP) fam- or Research Use Only. Not for Pily are involved in the breakdown of extracellular ma- FDiagnostic or Therapeutic Use. trix in normal physiological processes, such as embryonic Purchase does not include or carry development,reproduction, and tissue remodeling, as well any right to resell or transfer this product either as a stand-alone as in disease processes, such as arthritis and metastasis. product or as a component of another Most MMP’s are secreted as inactive proproteins which are product. Any use of this product other activated when cleaved by extracellular proteinases. The than the permitted use without the function of Matrix metalloproteinase 19 (MMP19, formerly express written authorization of Allele called MMP18) has not been determined yet. The MMP19 Biotech is strictly prohibited codes for four different isoforms (rasi-1, rasi- 3, rasi-6 and rasi-9). Variant rasi-1 encodes the full length protein whereas rasi-3 uses an alternate start codon and Website: www.allelebiotech.com alternate splicing site within the coding sequence resulting Call: 1-800-991-RNAi/858-587-6645 in a much shorter protein which is translated in a different (Pacific Time: 9:00AM~5:00PM) frame. In comparison to rasi-1, rasi-6 is alternatively spliced Email: [email protected] in exon 6 producing a change in frame and a premature stop codon in exon 7. Variant rasi-9 is alternatively spliced in exon 2, producing a different in-frame start codon compared to the full length transcript and encoding a distinct 12 amino Box 1 | Basic Info acid sequence at the N-terminus of the protein, followed by sequence identical to the full length protein. Both, rasi-1 and Cat. No. ABP-PAB-10865 rasi-9 are recognized by this antibody whereas rasi-3 and Animal ID RB2035/2036 rasi-6 are not. Host Rabbit Reactivity Human Buffers Format Purified Purified rabbit polyclonal antibody supplied in PBS with Accession number NM_002429 0.09% (W/V) sodium azide. This antibody is purified through Amount 100µg a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then fol- Alternative Name(s): lowed by dialysis against PBS. matrix metalloproteinase 19, MMP18, RASI-1, matrix metalloproteinase 18, MMP19 Immunogen KLH conjugated synthetic peptide comprised of amino acids 359 - 373 [RYINFKMSPGFPKKL] of the human matrix me- talloproteinase 19 (MMP19) protein. References:

1. Mauch S, Kolb C, Kolb B, Sadowski T, Sedlacek R: Application Matrix metalloproteinase-19 is expressed in myeloid cells in an adhesion-dependent manner and associates with the Tested by peptide-specific ELISA (1:1,000). IHC (1:50 cell surface. J. Immunol. 168(3): 1244-1251 (2002). ~1:100) 2. Stracke JO, Fosang AJ, Last K, Mercuri FA, Pendas AM, Llano E, Perris R, Di Cesare PE, Murphy G, Knauper V: Matrix 19 and 20 cleave aggrecan and cartilage oligomeric matrix protein (COMP). FEBS Lett. Storage 478(1-2): 52-56 (2000). Maintain refrigerated at 2-8ºC for up to 6 months. For long 3. Pendas AM, Knauper V, Puente XS, Llano E, Mattei MG, term storage store at -20ºC. Avoid repeated freeze-thaw Apte S, Murphy G, Lopez-Otin C: Identification and charac- cycles. terization of a novel human matrix metalloproteinase with unique structural characteristics, chromosomal location, and tissue distribution. J. Biol. Chem. 272(7): 4281-4286 (1997).

4. Cossins J, Dudgeon TJ, Catlin G, Gearing AJ, Clements JM: Identification of MMP-18, a putative novel human matrix metalloproteinase. Biochem. Biophys. Res. Commun. 228(2): 494-498 (1996).

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