NFASC Rabbit Pab

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Leader in Biomolecular Solutions for Life Science NFASC Rabbit pAb Catalog No.: A3053 Basic Information Background Catalog No. This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple A3053 IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on Observed MW axons during early development. Both the AIS and nodes of Ranvier contain high 150kDa densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 Calculated MW (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the 69kDa/119kDa/130-150kDa intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. Category Primary antibody Applications WB Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 23114 O94856 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 830-1100 of human NFASC (NP_001005388.2). Synonyms NFASC;NF;NRCAML Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using NFASC antibody (A3053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
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Targeted Genes Common Elements In
Table SV. Key TFs and their target DEGs in hub modules. Key TF Targeted genes Common elements in ‘DEGs’ (module) and ‘Targeted genes’ JUN HOMER2 ATF3 VEGFA FOSB NR4A3 MAFF ETS2 MAFF ETS2 KLF10 SESN2 (Purple) JOSD1 ATF3 RARA ATF3 BCOR DDIT4 IER2 GADD45B IER2 GPT2 LONRF3 MIDN HERPUD1 NDNL2 JUNB NR4A2 PHACTR3 DDIT4 ING1 SKP2 FOSL1 RARA AGPAT9 SLC7A1 NR4A2 SIAH2 CDKN1A VEGFA ATF3 BCOR ING1 BHLHE40 METRNL JOSD1 RARA VIT GRIA2 PPP1R15A BHLHE40 CHAC1 PKNOX2 PLCB4 PHF13 SOX9 SYT2 MIDN SOX9 ITPRIP KLF4 BCOR FOS AK5 GADD45B DUSP1 IER2 FOS CDKN1A JUNB STX11 PELO AVPI1 COL7A1 FAM131A TBCCD1 GRIA2 ERLIN1 HERPUD1 SIK1 GPRC5A C1QTNF7 AVPI1 KCNJ15 LATS2 ARC KLF4 BHLHE41 RELT DUSP2 VEGFA FOSB ZC3H12A LMO2 PELO SIK1 LONRF3 SPRY4 ARHGEF2 ARHGEF2 C1QTNF7 TFPI DUSP2 PKNOX2 RGS17 KCNJ15 LPAL2 FOSL1 HK1 NRCAM GPRC5A PPP1R15A CERK DENND3 RARA AGPAT9 DUSP1 CCNA2 SERTAD3 NR4A2 ACVR1B RARA SIAH1 RASSF5 CERK ZNF331 AK5 RELT BRD2 KCTD21 SKP2 NPAS2 ITPRIP NPAS2 SMOX RBM24 MIDN CCDC85C DUSP2 CARS EGR1 SESN2 RASSF5 ASNS FOS FOSB CCNA2 PIP4K2B SPRY4 ANKRD52 BCOR SIAH2 LMO2 DENND3 NR4A3 VIT TNFRSF1B ASTN2 PHACTR3 ASNS FEM1C TNFRSF1B SNORA80B CSRNP3 ITPRIP BNIP3L ATF3 ZNF331 RARA ZC3H12A CHAC1 ASTN2 VEZF1 DUSP1 SNORA80B KLF10 LPAL2 UBE2O EGR1 NR4A2 PCK1 COL7A1 PCK1 STX11 RRAGC PCK1 RBM24 GPT2 HOMER2 METRNL ARC BHLHE41 GARS C15orf41 FOS C1QTNF7 NPAS2 CSRNP3 NRCAM RGS17 VIT RGS17 UBE2O SOX9 KCTD21 NR4A3 RARA SMOX RELT GADD45B ATF3 SERTAD1 RARA BCOR BHLHE40 JUNB TINAGL1 ETS2 KLF10 GADD45B (Purple) LONRF3 COL7A1 TNFRSF1B MMP13 ATF3 SLC2A1 PIM2 CDKN1A ZC3H12A VEZF1 ARC ANKRD52
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Additional Tables.Xlsx
Additional Table 6 Enriched pathways of downregulated DEGs after spinal cord injury in CRMP2KI (P < 0.05, q < 0.05) Database Description Ratio of DEGs Ratio of universe P -value P adjust q value Gene name GO neuron to neuron synapse 52/523 379/11234 1.49E-12 7.29E-10 5.98E-10 Drp2/Grik5/Tnr/Cpeb4/Cdh2/Snx27/Pde4b/Srcin1/Slitrk1/Arrb1/Add3/Cdkl5/ Neurl1a/Adgrl1/Dlg4/Pkp4/Sorbs2/Arhgap44/Pip5k1c/Ppp1r9b/Gphn/Lrrc4c/ Adam10/Ntrk2/Anks1b/Kalrn/Arhgef9/Abhd17a/Adcy1/Cnih2/Baiap2/Add1/P alm/Slc30a3/Rpl12/Dgkz/Mapk1/Map2k1/Rps18/Sptbn1/Arc/Rpl14/Atp1a1/R pl8/Nsf/Rps19/Camk2n1/Syt11/Syt1/Rps14/Nrgn/Rpl38 GO postsynaptic density 49/523 357/11234 6.77E-12 1.51E-09 1.24E-09 Drp2/Grik5/Cpeb4/Cdh2/Snx27/Pde4b/Srcin1/Slitrk1/Arrb1/Add3/Cdkl5/Neur l1a/Adgrl1/Dlg4/Pkp4/Sorbs2/Arhgap44/Pip5k1c/Ppp1r9b/Gphn/Lrrc4c/Ada m10/Ntrk2/Anks1b/Kalrn/Arhgef9/Abhd17a/Adcy1/Cnih2/Baiap2/Add1/Palm/ Rpl12/Dgkz/Mapk1/Map2k1/Rps18/Sptbn1/Arc/Rpl14/Atp1a1/Rpl8/Nsf/Rps1 9/Camk2n1/Syt11/Rps14/Nrgn/Rpl38 GO asymmetric synapse 49/523 360/11234 9.23E-12 1.51E-09 1.24E-09 Drp2/Grik5/Cpeb4/Cdh2/Snx27/Pde4b/Srcin1/Slitrk1/Arrb1/Add3/Cdkl5/Neur l1a/Adgrl1/Dlg4/Pkp4/Sorbs2/Arhgap44/Pip5k1c/Ppp1r9b/Gphn/Lrrc4c/Ada m10/Ntrk2/Anks1b/Kalrn/Arhgef9/Abhd17a/Adcy1/Cnih2/Baiap2/Add1/Palm/ Rpl12/Dgkz/Mapk1/Map2k1/Rps18/Sptbn1/Arc/Rpl14/Atp1a1/Rpl8/Nsf/Rps1 9/Camk2n1/Syt11/Rps14/Nrgn/Rpl38 GO postsynaptic specialization 50/523 382/11234 2.41E-11 2.95E-09 2.42E-09 Drp2/Grik5/Cpeb4/Cdh2/Snx27/Pde4b/Srcin1/Dlgap3/Slitrk1/Arrb1/Add3/Cdk l5/Neurl1a/Adgrl1/Dlg4/Pkp4/Sorbs2/Arhgap44/Pip5k1c/Ppp1r9b/Gphn/Lrrc4
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NFASC Antibody
Product Datasheet NFASC Antibody Catalog No: #31250 Package Size: #31250-1 50ul #31250-2 100ul Orders: [email protected] Support: [email protected] Description Product Name NFASC Antibody Host Species Rabbit Clonality Polyclonal Applications WB Species Reactivity Hu Ms Rt Specificity The antibody detects endogenous level of total NFASC protein. Immunogen Type Peptide-KLH Immunogen Description Synthetic peptide corresponding to a region derived from 1225-1240 amino acids of human neurofascin Target Name NFASC Other Names neurofascin, NF, NRCAML Accession No. Swiss-Prot#: O94856; NCBI Gene#: 23114; NCBI Protein#: NP_001005388 Calculated MW 0.6mg/ml Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C/1 year Application Details Predicted MW: 150kd Western blotting: 1:200-1:1000 Images Gel: 6%SDS-PAGE Lysate: 40 ΞΌg Lane: Human lung tissue lysate Primary antibody: 31250(NFASC Antibody) at dilution 1/ 200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes Background This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 intracellular cytoskeleton.
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