MAPK8 Antibody Cat. No.: 62-956

MAPK8 Antibody

Confocal immunofluorescent analysis of MAPK8 Antibody MAPK8 Antibody immunohistochemistry analysis in with HepG2 cell followed by Alexa Fluor 488-conjugated formalin fixed and paraffin embedded human breast goat anti-rabbit lgG (green).DAPI was used to stain the cell tissue followed by peroxidase conjugation of the nuclear (blue). secondary antibody and DAB staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat

This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 358-389 amino acids from the C-terminal region of human MAPK8.

TESTED APPLICATIONS: IF, IHC-P, WB

September 24, 2021 1 https://www.prosci-inc.com/mapk8-antibody-62-956.html For WB starting dilution is: 1:1000

APPLICATIONS: For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

PREDICTED MOLECULAR 48 kDa WEIGHT:

Properties

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: MAPK8

Mitogen-activated protein 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated ALTERNATE NAMES: protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C

ACCESSION NO.: P45983

PROTEIN GI NO.: 2507195

GENE ID: 5599

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 24, 2021 2 https://www.prosci-inc.com/mapk8-antibody-62-956.html JNK1 is a member of the MAP kinase family. MAP act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early expression in response to cell stimuli. The activation of BACKGROUND: this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF- alpha induced . This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in proliferation, apoptosis and differentiation.

REFERENCES: 1) Deng, Y., et al., Cell 115(1):61-70 (2003).

2) Luo, W., et al., J. Biol. Chem. 278(39):37451-37458 (2003).

3) Ghosh, J., Biochem. Biophys. Res. Commun. 307(2):342-349 (2003).

4) Utsugi, M., et al., J. Immunol. 171(2):628-635 (2003).

ANTIBODIES FOR RESEARCH USE ONLY.

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