MAPK8 Antibody Cat. No.: 62-956
MAPK8 Antibody
Confocal immunofluorescent analysis of MAPK8 Antibody MAPK8 Antibody immunohistochemistry analysis in with HepG2 cell followed by Alexa Fluor 488-conjugated formalin fixed and paraffin embedded human breast goat anti-rabbit lgG (green).DAPI was used to stain the cell tissue followed by peroxidase conjugation of the nuclear (blue). secondary antibody and DAB staining.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat
This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 358-389 amino acids from the C-terminal region of human MAPK8.
TESTED APPLICATIONS: IF, IHC-P, WB
September 24, 2021 1 https://www.prosci-inc.com/mapk8-antibody-62-956.html For WB starting dilution is: 1:1000
APPLICATIONS: For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
PREDICTED MOLECULAR 48 kDa WEIGHT:
Properties
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: MAPK8
Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated ALTERNATE NAMES: protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C
ACCESSION NO.: P45983
PROTEIN GI NO.: 2507195
GENE ID: 5599
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
September 24, 2021 2 https://www.prosci-inc.com/mapk8-antibody-62-956.html JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of BACKGROUND: this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF- alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
REFERENCES: 1) Deng, Y., et al., Cell 115(1):61-70 (2003).
2) Luo, W., et al., J. Biol. Chem. 278(39):37451-37458 (2003).
3) Ghosh, J., Biochem. Biophys. Res. Commun. 307(2):342-349 (2003).
4) Utsugi, M., et al., J. Immunol. 171(2):628-635 (2003).
ANTIBODIES FOR RESEARCH USE ONLY.
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September 24, 2021 3 https://www.prosci-inc.com/mapk8-antibody-62-956.html