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Find the Baker Company SterilGARD VBM-600 at our website: Click HERE OPER~~TOR'S MANUAL Baker SterilGARD®II Biological Safety Cabinet
MODELS SG400STD, SG600STD, SG400ULT , SG600ULT
THE BAKER COMPANY
NSF classification: Class II, Type A. (When the unit is vented to the outside, the classification is Class II, Type B3).
This manual includes information for installation, operation, maintenance and spare parts. We recommend that it be kept near the cabinet for ready reference.
November, 1998 Revised: 10-2009 THE BAKER COMPANY
INTRODUCTION AND WELCOME It is a pleasure to welcome you to the growing number of customers who own and operate Baker biological safety cabinets. As the inventors of the laminar flow biological safety cabinet and the leaders in the field, Baker people take special pride in providing a cabinet which is designed for maximum performance. Your new SterilGARD®ll has a number of remarkable features. Among them, the contaminated positive pressure areas, which include the work area, are surrounded by a negative pressure plenum. Tilis arrangement provides an extra measure of protection because any particle in the contaminated zone will invariably be drawn through the blower and trapped on a filter. You will find your SterilGARD®II cabinet suitable for use not only with l.V. drug preparations and other pharmaceuticals, which could have adverse health effects on operators, but also for clinical diagnostic work involving tissue culturing of possibly infectious samples and other techniques requiring a contamination-free ahnosphere. Please note that all open-front containment cabinets, including this one, are for use with low to moderate risk agents only. Open-front cabinets do not provide absolute protection for the user. The adequacy of a cabinet for user safety should be determined on-site by an industrial hygienist, safety officer or other qualified person. Remember that you, the owner and user, are ultimately responsible and that you use your cabinet at your own risk. Built to exceed all microbiological aerosol tests specified by applicable NSF standards, your SteriJGARD"'l! unit includes many unusual Baker features which are included for superior performance, simpler maintenance and lower life cycle cost. Your SterilGARD8 II mut .is designed for both safety and value. We recommend this manual, along with the factory test report that is included, be kept near the cabinet for convenient reference by operators and qualified maintenance personnel. If you have any questions about the use or care of your new SterilGARD"'ll cabinet, please do not hesitate to contact our Customer Service Department for assistance.
SiHcerely,
Dennis Eagleson President The Baker Company, Inc.
P.O. Drawer E, Sanford, Muine 04073 (207) 324-87731-800-992-2537 FAX (207) 324-3869 "Creating immaculate atmo.rpheres"
1 TABLE OF CONTENTS
IN1RODUCTION ...... Page i
I FUNCTION AT\JD DESCRIPTION OF THE STERILGA.t~D'II CABINET...... 1 Airflow Inside the Biological Safety Cabinet ...... 1 Positive and Negative Pressure Areas ...... 2 Access to the Work Area ...... 2 Design Details ...... 2 SpeG "f" 1cahons ...... 1~ II PREPARING THE STERILGARD®II CABINET FOR USE ...... 5 Checking the Cabinet on Arrival ...... 5 The Uses of a Biological Safety Cabinet ...... 5 Cautions ...... 5 Location Within the Laboratory ...... 5 Installing the Cabinet ...... 6 Assembling the Footrest ...... 7 Connecting the Exhaust ...... 9 Final Connections and Tests ...... 9 III PROPER CABINET USE ...... 11 Operator Controls ...... 11 Air Pressure Monitor /Magnehelic Gauge ...... 12 Ground Fault Interrupter ...... 13 Start-up Procedure ...... 13 Working in the Cabinet Work Space ...... 13 Reacting to Spills ...... 14 Ultraviolet (Germicidal) Light ...... 15 Decontamination ...... 15 Using Ancillary Equipment ...... 17 About the HEPA Filters ...... 17 Operating Procedures ...... 18 IV ON-SITE CHECKS AND MAIN"TENAl\JCE ...... 19 The Airflow Balance ...... 19 Filter Media and Seal Leak Tests ...... 20 Airflow Smoke Pattern Test ...... 20 Cabinet Integrity Test ...... 20 G roun, d. mg r~on f rnm··t y ~-l es·t ...... "1~ fv1aintenance ?-.Jotes ...... ::?.1 Replacing the HEP A Fil ten; ...... 21 Trc,1Jbleshc)oti11g ...... 23
ll APPENDIX ...... Page 26 Wiring Diagram, SG400 Models ...... 26 Wiring Diagram, SG600 Models ...... 27 Dimensional Drawing, Model SG400STD ...... 28 Dimensional Drawing, Model SG600STD ...... ;...... 29 Dimensional Drawing, Model SG400UL T ...... 30 Dimensional Drawing, Model SG600ULT ...... 31 Replacement Parts List, Models SG400STD & SG600STD ...... 32 Replacement Parts List, Models SG400UL T & SG600UL T ...... 33 Warranty ...... 34
LIST OF FIGURES
Fig. 1 Airflow Inside Cabinet ...... 1 Fig. 2 Adjusting the Telescoping Leg Assembly ...... 6 Fig. 3 Installation Without Lifting Equipment ...... 6 Fig. 4 Assembling the Footrest ...... 8 Fig. 5 Operator Controls ...... 11
111 I -FUNCTION AND DESCRIPTION OF THE STERILGARD®II CABINET
The SterilGARD"'II is a biological safety cabinet of original design. It features vertical laminar airflow and a front access opening. The ru1it is designed to protect not only the environment and Exhaust the people using the cabinet, but also the product Blower )I> filter within from airborne contaminants.
P!enum Airflow Inside the Biological Safety Cabinet
The SterilGARD® II cabinet features The Baker Supply Company's zoned airflow principle. See Fig. 1. filter The stainless steel metal diffuser just below the supply filter creates a faster airflow at the front of ~ the work zone than at the center. The protective faster airflow in front makes an extremely v v v effective air barrier. ~ A feature of the unique Baker design is the high <} v v v volume return air slots, which maximize the t::J;;J ~ 8" access opening cabinet's protective capability. It is generally ;;J ---- accepted that maintaining containment and a particle free work area is most difficult in the area t in whim airflow turbulence is greatest- at the - - intersection of the side walls, the front access opening and the work surface. Turbulence caused by friction will also be found along a cabinet's side walls. ln cabinets without high velocity return air slots, this turbulence may allow contaminants to escape from the work area, or it may make it possible for unfiltered room air to enter the work area. The Baker high-velocity retu.m air slots are located along the side walls of the work area. Air Room air is dravv:n into the slots at very high speed, negative pressure preventing the escape of particulates and + Contaminated air ensuring that no unfiltered air enters the work positive pressure area. Additional hjgh-velocity return air slots are <> Clean air located at the top and sides of the viewscreen to .qp. Contamina~ed air prevent gases, \'·apors or particulates from con1ing negative pressure up behlnd the vie\vscreen and escaping into the laboratory. In the same \va.L they' prevent room air .frorn do>A'T1 behJnd tl1e \/ie'i\rscreen and contarninatLTlg the work area. Fig. 1 Airflow Inside Cabinet
1 Positive and Negative Pressure ~<\reas ho\v to use the ·v-ieTNscreen correctly vvlli]e the cabinet is in use.) One of the n1any features that sets the SterilGARD"':-n unit apart frorn oL~er cabinets is As with other Baker cabinets, the electricctl the interaction of positive cu1cl negative pressure outlets Lnside the \Vork. area have a circuit bre::tker areas. The srrtalJ posibve pressure pienwn is so that an electrical overload by ancillary cornpleteJy surrollnded by a negati'i/e pressure equipment will not affect air handling. area so that any partide passing through is Overloading with electrical equipment should, of invariably drawn thrm1gh the blower and trapped course, be avoid.ed in any case. (See th.e in a filter. "A.ncillary Equipment" instructions in Chapter 3 on "Proper Cabinet Use", and in the "Appendix.") From the small positive pressure plenum, which is shown .in Figure 1, about 30 percent of Ll-te total cabinet airflow is expelled through the exhaust Design Details filter. The remaining 70 percent is recirculated Performance assurance through the supply filter and re-enters the work Meticulous care in manufacturing is followed by area as particle-free air. Because the cabinet must more than 14 separate performance tests prior to take in air to replace the air expelled through the shipment of your cabinet. In addition, a complete exhaust filter, the same volume of room air enters factory test report on the performance of your the cabinet through the 8-inch front access unit is included at the back of this manual. opening. This air, which never enters the work area, cornp letes the air bar.rier at the front access Motor/ blower capacity opening. A motor/blower's capacity is measured by its All air is combined under the work surface. ability to provide a nearly constant volume of air From here, the contaminated air is pulled, under as resistance increases becau.se of filter loading. negative pressure, through a plenurn up the rear Verification by a simulated filter loading test has of the cabinet into the motor-blower, which blows established that your SteriiCARD"Il cabinet is it into a positive pressure plenum. Please note capable of auto~atically handling an 80% (in both again that the positive pressure plenum is 4-foot and 6-foot 1mit) increase in pressure drop completely surrounded by negative pressure across the filter without reducing total air delivery areas. If a leak should occur in a contaminated more than 10~/o. A convenient magnehelic gauge negative pressure plenum, or in the side wall or (or bar graph display on the ULTRA models) rear wall, the negative pressure will create suction shows the current relative performance of the and pu !I air in, not allowing it to escape into the blower. With the use of the manual speed control, operator's area. If there is a leak from the positive a 165');,/180% (4-foot/6-foot unit) increase in the pressure plenum, the surrounding negative pressure drop across the filter can be handled. pressure area will recapture the contamicnated air The resulting long filter life means significant cost and recirculate it through a filter. savings.
Access to the Work Area One-piece interior wall construction For easy entry of apparatus into the work area, The interior side and rear walls of your SterilGARD"'ll has a vertical sliding.. viewscreen. Steri!GARD®Il's work area are constructed from a Its \4-inch safety plate glass allows excellent single piece of 16-gauge stainless steel. The easy visibility and may be opened to a height of 18 W' to-clean 7 /16-inch radiused (rounded) comers to permit placement of items in the work area. prevent buildup of contaminants and resist TI1e window should be set at a height of 8" while corrosion. work is being performed, and an alarm is provided to remind the operator if the window is Protective screen not in the correct position for using th.e cabinet. Located under the work surface at the bottom (Chapter 3 on "Proper Cabinet Use'' will explain and sides of the rear return-air plenum, a protective screen is provided to help prevent Work area lighting wipes and other paper materials from being SterilGARD"'II's lighting system produces 100 to drawn into the blower system. This precaution 150 foot candles of illumination at the work eliminates costly decontamination and downtime. surface area. The fluorescent tight is extemall.y The screen affords easy access to waste materials m01mted to minimize heat buildup. The unit uses and should be kept clean at all times. two common fluorescent bulbs. All-metal plenums Air balance adjustments The plenums of your SterilGARD"II unit have Air balancing can be done by either of the been constructed entirely of metal in order to following methods. It should, however, be done provide strength, durability, air-tightness and only by a teclmician with proper training and resistance to deterioration. equipment. (See Section 4, "On-Site Checks and Recessed stainless steel work surface Maintenance Procedures.") The work surface is constructed of corrosion • A speed controller adjusts for voltage resistant 16-gauge type 304 stainless steel. Its differences and filter loading. satin finish diminishes light reflection. It is • An adjustable damper compensates for recessed to retain spills, and the 3/16" radiused differences of supply and exhaust filters if con1ers make for easy cleaning. they are changed at a later date.
Cabinet exterior Easy filter access Extemal construction is of 14-gauge cold-rolled For convenience and ease of service, the supply steel, protected by a smooth white baked enamel and exhaust filters are loaded and removed from finish. the front of the cabinet without entry into SterilGARD'''Il's work area. This is particularly Tested HEPA!ULPA filters helpful if the cabinet is connected to an exhaust Supply and exhaust filters in SterilGARDeii are duct. Filters should be replaced by qualified zero-probed HEPA. They have been found to be technicians on! y. 99.99% effective on particles of 0.3 micron size. In Stainless steel telescoping leg assembly the SterilGARD''II ULTRA 4-foot model only, supply and exhaust ULPA filters are used, whicll TI1e Steri!GARD"II unit has a distinctive are 99.999'Yo effective on particulates of 0.12 assembly with four telescoping legs, whicll allows micron. Eacll filter is scan-tested to assure leak the cabinet to be shipped with a minimum height free installation. of 74 7/8". The assembly also makes it possible to adjust the cabinet to two heights, providing a Viewscreen work surface of either 30" or 36" above the floor. The cabinet's %-incll safety plate glass vertical sliding viewscreen may be opened to 18 Yz" for Leg I Knee Work placement of large items in the work area, and Exten- Space Surface Overall may be fully closed for system shutdown. sion I Height Height Height
< Shipping & 11~~./-,; " - - Drain valve I 74 cIs" fnstallation I I I MeetL"1g NSf requirements for drainage in Class Setting A i 25'" ) 26''* 30'''!" 891/o" i ' I I II biologicaJ safety cabh"'"tets, a stainless stee] baJJ ~ 'I ., ' 0" •• / Settmg B ' .. ) ""! • ! /~:;, :/ b 1! >.raJve i_n the SterJJGARD~]l unit is installed for sc:Je effective d.rainage of the drain pan. levelerc; Specifications Weight The weight of the SterilGARD'"II 4-foot cabinet is 630 pounds with a shippirtg weight of 875 lbs. A 6-foot cabinet weighs 840 pounds with a shipping weight of 1075 pounds.
Electrical specifications 115V AC, 1 Phase, 60 Hz The SterilGARD0 11 incorporates a solid-state controller for the blower motor. This compensates for variations in incoming line voltages. The usable voltage range is 95 to 130V AC The unit is provided with two duplex G.FJ. receptacles at a total of 5.0 amps, controlled by an internal self-resetting circuit breaker. The unit is furnished with one 12' power cord with 20-amp plug, type NEMA 5-20P.
SG400/SG600 Blower motor (1 ,625 RPM) 6.6 amps/9.9 amps Fluorescent Light (ballast) 0.7 amps/0.7 amps Duplex outlets (G. F.!.) 5.0 amps/5.0 amps Total running load 12.3 arnps/15.6 amps
4 II - PREPARING THE STERILGARD®II CABINET FOR USE
Checking the Cabinet On Arrival consult your safety professional for a proper risk assessment. Upon receipt of your new SteriiGARDoll cabinet, first inspect the exterior of the crate and skid. If there is any broken glass or other visible C4.UTIONS damage, that fact should be noted on the receiving slip and immediately reported to the * T1w use of any hazardous material in the delivering carrier. cabinet requires that it be monitored by an industrial hygienist, safety officer or other Now remove the crate and inspect the tmit qualified i>u/ividual. itself. The top cover of the crate should be taken * off first, then the boards from front and back. Explosive or flammable su/Jstances should Bend both ends of the crate outward away from never be used in the cabinet unless a quali the sides of the unit and remove front or rear fied safety professional has evaluated the risk. blocking from the skid. Remove the cabinet from the skid with a fork lift or other available * lf hazardous biological work is to be per equipment. If any concealed damage is found it formed, apply the app1·opriate biohazard de should be reported to the delivering carrier, who cal which is enclosed. This is in accord with will want m1 opportunity to inspect the damage. OSHA regulations, volume 39, 11umber 125, A claim for restihttion shollld be filed within 15 PadiL days. * If chentical, 1·adialogical or other n.on.micro . Because of the dwger of mishandling by bialogical hazards are present, be sure to trucking companies, we have removed certain employ appropriate protective measures in parts of the cabinet and have packed them addition to formaldehyde decontamination. separatelv. These items are listed on the packing Call upol! a suitably trailred individual to slip which accompanies the unit. Please check the monitor the operation. packing slip carefully to be sure that all items have been located. Location Within the Laboratory The ideal location for any laminar flow The Uses of a Biological Safety Cabinet biological safety cabinet is in a dead-end comer of The SterilGARD"II cabinet has been designed to the laboratory away from personnel traffic, vents, provide a work area which protects the doors, windows and any other sources of experiment from the environment, and the dismptive air currents. Published research from enviroruncnt frorn the experi-ment. The larninnr The Baker Companv (Rake, B.W. "Influence of flow biological safety cabinet is designed for work Crossdrafts" on the Perfonnance of a Biological with Biosafety Levels 1, 2 and 3 (low to moderate Safety Cabinet. Appl. Microbiology 36:278-283, risk) agents as listed in The Center for Disease 1978.) and w1published tests performed at the Control's ~~Biosafety in lviicTobiological and National Cancer Institute show that i.f a draft or Biomedical Laboratories", U.S. Department of other disruptive air current were to exceed the Health and Hllman Services, Public I-Iealth intake velocity of the cabinet, then contarrlination Service, Centers for Disease Control and National can e-nter the vvork area or escape frorn it. Proper Institutes of Health, L.S. Govenunent Printing placement within the is essential. Office, Washington, D.C. 20402. HHS publication If the cabinet exhausts Lts air into the Iaborrltonr nurnber (CDC! 34.-3395. ins:tead of venting to the OL.ltsidef it is imoortant Biosafety level 4 or extremely f'Ligh risk agents that there be adequate space between th~ top of sl1ould neve·r be used in ul-lls cabinet. Please t._he cabinet and the ceiling. A soJjd ceiling located
5 too close to the exllaust filter vvill restrJct the air and limit the intake velocity . .t., mi_ni.mum of 12" of clearance on all sides and top of the cabinet is necessary to achieve ideal conditions for testing. If a free space of 12" above the exhaust filter is not 0 available, inaccuracies in exhaust flow J.l ~\ measurements mav result. Consult with our CJ 0 Customer Service Department for the implications of this, and for alternatives. vlegbolt Installing the Cabinet Installation of this cabinet should be carried out ,, in accordance with appropriate OSHA regulations \\ -'-8" lift and those of other regulatory agencies having jurisdiction. • Fig. 3 Installation Without Lifting Equipment 1. First, move the cabinet from the unloading area to its intended location on dollies. The overall dimensions in the shipping position for the ·!-foot unit are: 34 :I 12" deep x 53 3 I 16" 2. For convenience in moving the cabinet, its 7 wide x 74 Is" high. For the 6-foot unit, the depth may be reduced to 31 7Is" by removing 1 overall shipping dimensions are: 34 /32" the light canopy assembly. First, with the deep x 77 '/1s" wide x 74 7Is" high. unit disconnected electrically, remove the two nuts holding the canopy clips at the top of the canopy. Then let the canopy pivot down until the end restraints stop it. Disconnect the electrical plugs inside on each end. Disconnect the restraints and lift the canopy up and off. 111en move the canopy to a new location. Glass window ) / (tron1 of oabine1) 3. The unit can be assembled with the work surface either 30" or 36" from the floor
Work surface opening according to the needs of the owner. The unit lower edge is shipped with the legs extending 6" below t.lte cabirtet. If lifting eqvipm-~nt js available_. lift the cabinet vertically to a height of approximately 40." All four legs may then be extended to the desired height by removing the four shipping bolts, setting the height of the legs and then securing the legs in position with the four bolts which are supplied with the unit eIs" X 2 3 I 4" - see Figure 2). use a 9 /16" wrench. TI1e cabinet must be held safely at the •±0" height duri.rtg the entire operation, and set back onto the floor only after all four legs have been secured. Final leveling may Fig. 2 Adjusting the Telescoping Leg Assembly
6 be accomplished with the adjusting leveling 8. Check to be sure that the liquid drain valve is pads, located at the bottom of each leg. in the closed position. (The handle on the 4. If lifting equipment is not available, the legs valve should be parallel to the floor). If rna y be extended to the desired height in the something is spilled in the work area, it will following manner. It is recommended that at remain in the drain·system and wo11't reach least four people be used for this operation. the laboratory floor. Precautions may be required for the safe disposal of the effluent
. from the drain. WA.RNING! 9. Also check to see that service petcocks, if The 4-foot SterilGARD@II weighs 630 lbs. present, are in the closed position. (The (6-foot unit weighs 840 lbs.). ilt least four handle on eilch valve should be people should be used to perf01~1t the perpendicular to the rear wall of the unit if following pl'ocedure. . installed in the sidewalls). 10. Remove the hardboard (masonite) shipping Lift either the right or left end of the cabinet cover from the top of the ex.l-taust filter. approximately 8" to 9." See Fig. 3. Remove 11. Remove the viewscreen shipping clips and the shipping screws and lower the free legs to supports and carefully check the viewscreen the next hole position in tl1e leg. Then for smooth vertical operation. If the replace the shipping screws to the lock-in viewscreen binds, adjust the track by position. loosening the scre\1\'S, arranging the track in Repeat the step above by lifting from the the proper position, and then re-tightening to opposite end. This will bring the cabinet to a hold the track in the correct position. In level position. Extreme care should be taken order to be effective, the wiper gasket must in this step to make sure the cabinet does not exert a modest amotmt of "drag" on the glass. slip on the floor. If the view screen is loosened, make sure that the wiper still makes contact. Also remove Now repeat the steps above once to obtain the shipping screws and clips from the front the sitting position height and twice to obtain and rear perforated grills. the standing position height. :o. After the unit has been adjusted to the desired position, insert the solid dot plugs Assembling the Footrest (shipped in a small bag on the work surface), 1. Slide the right-hand and left-hand footrest into the unused holes in the legs to prevent tube brackets over the front adjustable legs as dust and dirt from entering the legs through shown in Fig. 4. the open holes. 2. Using the supplied hardware, secure each 6. The drain pan is located under the work h1be bracket to the nlounting hole. Each hole surface. During shipment, this work surface is located 1H 3 Is" from the floor. Insert the is held in place by hold-down clips. Lift the bolt from the rear, through the slot, and vvork surface and rernuve the hold-do\vn through the hole of the adjusting leg. Be sure clips. that the slot on the underside of the bolt fits 7. Level the work surface by adjusting the into the slot on the bracket. This will prevent glides on ea.ch of the four con.1ers of the ieg the bolt fron1 turning as the assernb.ly is: risers. Be sure that all four are finnly on the tightened. Slide the bracket 1.1p or dovvn so tloor .so that the ce1binet does not teeter. ~..\.n the bolt is centered Lrt the \/erticZll slot. finJ::.h adjustrnent range of 1 1/ .{" i.s provided. securing each bracket using a 3 Is" washer, a Adjust to obtain a work surface height of 30" '• lockvvasher! and a iocking ha_ndle_ to 36." The handles may be adjusted to manv positions. Pulling out on the handle
j disengages it while the threaded portion Set the footrest so the lower stud is centered remains stationary. Releasing the handle re in the radiused slot. Secure the lower stud engages it for tightening and loosening. The with the supplied hardware e/s" washer on handles for the tube brackets should be set in inside, 3/ s" lockwasher, and nut on outside). tlce vertical positions. 4. The height of the footrest may be adjusted 3. Find a helper to assist you. Slide the footrest over the range allowed by the slots on the between the legs of the unit. On each side, tube brackets. The angle of the footrest may place the upper part (3 /s" dia. hole) over the be adjusted over a range of 20°. upper stud. The curving slot should fit over the lower slot. On each side, secure the stud at the top with the supplied hardware (3/s" washer on inside, 3 /s" lockwasher, and nut on outside).
Upper stud: R. H. footrest 3/s" lockwasher tube bracket 3/s" washer 3/e'' nut
·_./r~f~ ~'>,..
/1:-/ .:.-::.....,:::-mw- 'i1 1i _.... '-!.- aj / ' ~;.--
./
L. H. footrest Footrest bolt tube bracket 3fs" flat washer " 3/a" lockwasher locking handle
Lower stud: 3/s" washer 3fa" lockwasher locking handle
Footrest bolt
Fig. 4 Assembling the Footrest
8 Connecting the Exhaust Exhaust to the outside - The SterilGARD@II cabinet can operate Whenever possible, the filtered exhaust with filtered exhaust air enteri11g directly should be cormected to its own separate i11to the room, or with filtered exhaust exhaust svstem. If it m11st be channeled into a duded to the outdoors. Details of these multi-du~t system, make sure that the'system alternatives are as follows: is not a recirculating one. You will, of course, also want to make sure that the system can Exhaust into the room - handle the volume of air required to pass through it, and that there is sufficient static If the cabinet exhausts its air directly into the pressure for proper cabinet function. laboratory instead of ducting it to the outside, The exhaust requirements of the 4-foot it is important that there be adequate space between the top of the cabinet and the ceiling. SterilGARD'fl are 268 CFM at .02"-.04" water If the solid ceiling is located less than 4" from column suction directly above the exhaust the exhaust filter, it may restrict the air and filter before any reductions, elbows or other limit the cabinet's intake velocity. restrictions. If using Baker's hard exhaust transition (ET400), add 0.10" water column. Although 4'" of top clearance may be If a Baker thimble exhaust transition is being sufficient for day-to-day operation of the used, 322 CFM minimum at 0.10" water cabinet, it will be difficult to obtain airflow column. testing information. At least 12" of clearance on the top of the cabinet is recommended for The exhaust reguirernents of the 6·-foot reliable rneasure.rnent of ai.rflovv. SteriiGARD-!1 are 408 CHv! at .02"-.04" water column suction directly above the exhaust Never use ttle top of the cabinet or the work filter before any reductions, elbows or other area for storage purposes, and never use restrictions. If using Baker's hard exhaust flac'Timable, explosive or toxic vapors or transition (ET600), add 0.10" water column. gases, or substances which generate them, If a Baker thimble exhaust transition is being unless a qualified safety professional has used, 462 CFM minimum at ()_22" water evaluated the risk The filter removes only column. particulates and not gases, thus causing the recirculation of these within the cabinet. You may want to install an indicator light or some other safety device to give warning if Attach the long-legged exhaust filter guard, the exhaust system fails. A properly located in the rear, to prevent inadvertent designed duct system includes an airtight blocking of the filter area. damper to balance the air and also to shut off Remove the two nuts from the back of the the duct for purposes of cabinet exhaust filter guard, and then remove nvo decontamination. The Baker Cornpanv offers nuts from the front and two from the rear of a transition duct piece for connecting the unit the exhaust filter housirtg on top of the unit. to the exhaLLSt system. Call for details. Install the legs on the studs at the rear of the For further infonnation, refer to the National exhaust filter housing. Tighten them dovvn Sanitation Foundation's Standard J"Jo. 49 and with the previously-removed nuts. other guidelines regarding ·ventilation. Install the exhaust filter guard, setting the front clips on the front studs of the exhaust Final Connections and Tests filter housing. Tighten then1 down vvith previously' rernoved nllts. 1. The pl'untbi_ng connection to the service petcocks. rrrust be made with great care I'-Jov~_.· secure the filter g-uard to the ='is-inch because the effluent from a safety cabinet NPT nipples in the top. rr1ay be biologically hazardous. VVhen present, petcocks are piped ·vvithin the
9 cabinet. The external connection uses 3/8- 7. Although all units are carefully tested at the inch NPT nipples at the base of the cab~cet. factory, it is advisable that certain other Connection to plant utilities should be made checks be made on-site by a qualified with proper materials and technique. No technician after installation. These include flammable gas should be used.• However, ii testing the filters for leaks and checking the the risk is professionally evaluated and a air balance of the unit, especially ii it is decision is made to install a flammable gas connected to an exhaust system. A petcock, then an emergency shut-off valve description of these tests can be found in should be situated in an accessible location Section 4, "On-site Checks and Maintenance." outside the cabinet. 8. It is also recommended that all personnel 2. A 20-amp power cord with a NEMA 5-20P who will be using the cabinet study this plug is furnished with the SterilGARD"'II. It Operator's Manual to learn how to make the should be plugged into an appropriate 115 most effective use of the unit. For additional Volt, 60 Hz, 20 amp dedicated utility outlet. and start up and use procedures, please turn 3. Press the blower switch. The yellow indicator to Section 3, "Proper Cabinet Use." above the switch should light. 4. Before using the cabinet, tum on the fluorescent light cmd make sure the bulb is lighted. These bulbs are locked into place FOR IviORE INFORMATION with stop-lock fittings. For a complete listing of articles, papers and 5. Allow the cabinet to run for about half an reports related to containment, clean ail· hour so the dirty air in the work area will be products and safety, contact The Baker purged. Company for our complete Bibliography or Leave the blower running and wash the visit our website at www.bakerco.com. entire cabinet, inside and out, with a detergent-disinfectcmt to remove surface dust. Once started, we recommend that all cabinets be left rurming continuously. For additional start-up and use procedures, please turn to Section 3, "Proper Cabinet Use." 6. The SterilGARD®ll cabinet has been subjected to a comprehensive series of physical tests before shipment from the factory. A physical test report is filed by serial number as a permanent record at Baker headquarters, and a copy of the report accompanies each shipment. Your copy is at the back of this manual.
10 m- PROPER CABINET USE
A biological safety cabinet is a valuable Operator Controls supplement to good sterile technique, but is not a The operator controls and indicators are replacement for it. If the cabinet is not arranged on the front panel of the unit. See Fig. 5. understood and operated correctly, it will not A number of switches are arranged in a single provide an adequate protective barrier. membrane switch assembly. All activities that are to be performed in your cabinet should first be approved by a competent 1 -Alarm Reset Switch/ Sash Level Alarm - professional, such as an industrial hygienist or For normal operation, the viewscreen must be safety officer, to make sure that the cabinet is placed to allow an opening of exactly 8." This appropriate for the work it wiJl be required to do. setting is important to allow the correct airflow This person should monitor the cabinet and its into the work area. The window may also be operating personnel at regular intervals to see that closed completely. If the window is left in any it is being used correctly. other position, an alarm will sound. An indicator ln order to keep the interior workspace clean above the alarm reset switch will flash. and free of particulates, all Baker laminar flow To mute the audible alarm, press the alarm reset cabinets are designed for continuous operation. If button. The indicator will continue to flash. After the blower is turned off, the unit becomes five minutes, the alarm will sound again to contaminated with room air. Therefore it is remind you to lower the window. You may press recommended that the blower be left on. the alarm reset switch again to mute the alarm for another five minutes. The alarm will sound again if the view screen is not in the p1·oper 8" position.
2 · Slower On/Off (blower on indicator· yellow) 3 -Outlets On/Off 1 - Alarm ReseV (oUllets on indicator- yellow) Sash Level Alarm
4- Light On/Off Air pressure monitor '\. (light on indicator- yellow) bar graph (mag gauge} , '\. S- UV Light On/Ott (UV light on indicator -yellow)
~ Light canopy
Fig. 5 Operator Controls
11 When the unit is operating, the viewscreen shows the relative cabinet perfonnance, measured must be open exactly 8", or must be completely in terms of the total volume of air being handled closed. At any other setting, the sash level a lam1 bv the unit. indicator will flash. The top of the lighted area indicates the relative airflow. When the airtlow is normal, the LEUs in 2- Blower On/Off Switch- the center of the bar graph will glow with a steady This switch controls the power to the blower for green light. the unit. When the switch is turned on, the If just one or two LED's at the bottom of the yellow indicator will light. The lights and outlets graph glow with a red light, the milt is handling a will not operate unless the blower switch is low volume of air. An alarm will also sound. turned on first. This indication mea11s that the unit is not The indicator light above the switch shows that receiving enough intake air. the switch has been turned on.
3- Outlet On/Off- .·· vV..4RNING! This switch controls the two outlets on each side If the unit continues to operate in this of the work area. When the outlets are turned on, condition, it is possible that contamination the yellow indicator above the switch will light. may spread outside of the work area. The product inside the work area may also be cmttil1n.ina.ted. If you see the- lo1.V airflow The indicator above the switch will light when indicator, take these steps: the outlets are turned on. • Lea fluoncscent J;ght. The fluorescent lab coats with tight-fitting cuffs and sterile light will not operate unless the ultraviolet gloves. This minimizes the shedding of skin J;ght is turned off. Never leave the flora into the work area and protects hands ultraviolet light 011 while there is anyone ilz and arms from contamination the roont. 2. Perfonn all work on the depressed area of the 3. Check to determine that the drain v'alve is in solid work surface. Work with a limited the closed position or the drain coupling is number of slow movements. Since all of the capped. equipnlent you need is already in tl1e cabinet, 4. \Alipe do·vvn the interior area of the cabinet it \Vili not be necessary to move your arms in vvith a surface disinfectant. and out through the air barrier. Note: Some disinfectants may corrode or 3. Because opening and closing doors in the stain the steel surfaces. If this happens, clean laboratorv causes air disturbcmce wluch might interfere with cabinet airflow, this kind
13 of activity should be kept to a minimum to nm for at least three minutes with no while the cabinet is in use. activity so that the airborne contaminants will 4. Avoid using floor-type pipette discard be purged from tbe work area. Next, make canisters. It is important that your used sure that all equipment is removed from the pipettes be discarded onto a tray or other cabinet. suitable container inside the cabinet. This 8. After you have removed all materials, culture reduces the temptation to move in and out of apparatus, etc., decontamination of the the work area unnecessarily. interior surfaces should be repeated. Check Because of tl1e restricted access, pipetting the work area carefully for spilled or splashed within the cabinet will require the use of nutrient which might support bacterial pipett:ing aids. growth. Never use the cabinet to store supplies or laboratory equipment. 5. Use good aseptic technique. Procedures done with good technique and proper cabinet 9. We recommend that the cabinet be left methods will not require the use of a flame. numing continuously to ensure containment and cleanliness. If the user elects to turn the If, however, a safety officer approves the use cabinet off at the end of a work session, the of flame after evaluating the circumstances, viewscreen should be closed completely. The then a burner with a pilot ljght such as the sash alaml will be silenced when the "Touch-0-Matic" should be used. Place it at viewscreen is in the closed position. the rear of the work area where the air turbulence caused by the flame wilt have the least possible effect. Flame disturbs the Reacting to Spills directional airstream and also contributes to 1. If an accident occurs which causes spills and tile heat load. If the cabinet blower is spatters arolll1d the work area, you will need unintentionally turned off, the flame could to decontaminate all items and surfaces also damage a filter. before any items are removed. If the spill Tubing for a burner within the cabinet should was enough to create puddles of liquid in the be resistant to cracking or pU11cture. Material drain pan, then an emergency spiU procedure such as Tygon tubing may not be acceptable should be followed. (This procedure should for this use. be established prior to an accident.) 6. Never operate your cabinet while the It is recommended that the researchers, in viezvscreen alarm iHdicator is on. The coordination with their consulting safety operating position of the sash provides an 8- professional, have a written plan available in inch high access opening. This restricted case of an accidental exposure or spill. The opening permits optimum operating safety p!Bn shoHld include all of the cuHditiou:; foL the cabinet. For operati.ttg ernergency procedu1es to be folloYved in t}'te comfort it is recorrunended that the top of the event of an accident. All employees should operator's shoulder be at the same height as be familiar with the emergency procedures. the bottom of the viewscreen. Because The emergencv spill procedure may vary operators wili not be the same height, it is an accord.ing to the agents being used. suggested that the operator use a chair which may be adjusted for height. 2. In the case of a biological spill, for example, the area containing the spi_U n1ay be flooded 7. ~-\fter a procedure has been cornpleted, all with an appropriate disinfectant. The drain equipn1ent 1-vhich has been !_n contact \Vith capacity of the SG400 rnodels is 19.S gallons, the research agent should be enclosed, and with the SG600 mode.\s having a 28.7 gal.lon the entire surface decontarninated. Trays of capacity. discarded pipettes artd glassv..'are should be covered. The cabinet should then be allowed iJter the disirlfectant has had tllne for a complete kill, remove or drain tbe residue. If
14 . you have used a disinfectant which is WA.RNLNG! harmful to stainless steel (Hypochlorite • Eyes and ski11 should not be exposed to solutions, for example) be sure that none dired ultraviolet light. remains to corrode cabinet surfaces. Clean the s>Jrfaces with sterile water. • Ultraviolet light should not be relied upon as . t~re sole decontamiltating agent. 3. If you have a spill involving a hazardous Addtiwnal sulface disinfection should be Biosafety Level 2 or 3 agent, then you are performed both before and after every advised to leave the cabinet running and cabinet usc. close the viewscreen, so as to let the aerosols • A biological safety cabinet acts as a sup settle before you start cleanup procedures. With some spills, it may be necessary to plement to good aseptic practices, 110t as a replacement. decontaminate the room with an ag~nt such as formaldehyde gas. (Biosafety Level 4 agents should never be used in this type of Decontamination cabinet.) , vVhenever maintenance, service or repair is 4. If the spill contains volatile liquids which needed in a contaminated area of vour cabinet generate vapors creating a danger of fire or the unit must first be bv an ' explosion, tum off the unit and other deconta:min~ted appropriate agent. The National InstitL;te of electrical appliances and close the viewscreen. Health, ~ational Cancer Institute and the Center Evacuate and seal tl1e room and call for for Disease Control have all recommended the use irrunediate help from a safety professional. of formaldehyde gas for most microbiological 5. lf the agent is a hazardous chemical, it may agents. Its application requires individuals who be recommended that a Spill Kit be kept are experienced in the decontamination of readily available. This kit should be clearly cabinets, since the gas itself is toxic. labeled, and might include such items as a A good reference for this procedure is The respirator, chemical splash goggles, two pairs National Cancer Institute's "Formaldehyde of gloves, two sheets of absorbent material, Decontamination of Laminar Flow Biological spill control pillows, a solution to clean the Safety Cabinets" (pamphlet and/ or slide contaminated area, and waste disposal bags program), U.S. Department of Health, Education or other conta.iners. Consult your safety and Welfare: National Institutes of Health. professional for proper procedures and Available through Chief of Sales Branch, '\Jational treatment of the specific agents you plan to Audiovisual Center, Washi11gton, DC 20409. use. An ethylene oxide gas mixture is an alternative, but it involves a more complicated procedure and should only be used by personnel ';.Vho are Ultraviolet (Germicidal) Light familiar with its operation. This SterilGARD"II unit has an ultraviolet light. Whatever gas you choose, have the proper safety equiprnent (gas masksr protective clothin(', The light is controlled by an on/ off switch on the . l . b front panel. See Fig. 5. \Nhen the fluorescent light e t c. ) \Nlt uneasy reach. ln addition, vou vvilJ vvant to be sure that the gas you are using.will be rs on, the ultraviolet light cannot operate. The ev effective against all of the biolcsical agents \vithin light \VilJ not operate unless the vievvscreen is the cabinet V\1hen you have dt;cid_ed gas to cornpleteJy dovv'n and t11e fluorescent light is off. ~rlrich use, post the antidote to it in a visible and n~~trb\r Cltraviolet lamps lose their effectiveness 0'/er location. The volurne of the SG-100 cabinet is 55 · tirr1e and should be replaced vvhen _inten::it\.r droD~ cubic feet ,_::ubic feet- for the SG600 L Pruvkie be.! ow tile optimum le,vel. Check regular.ly·. ' - tl1e correct arnowTt of deco.ntarrtinating gtts for tbis volurne. ,
15 Carcinogens present a unique chemical placed out a window to exhaust the deactivation problem and the standard biological formaldehyde gas. decontamination will not, be effective against 6. Place a heating device, such as a commer chemicals or other non-biological materials. With cially available electric frying pan or a remote materials of this kind, consult a qualified safety formaldehyde generator /neutralizer, with the professional. themwstat set at 450 to 475'F (232.2 to 246.1°C) on the work tray. The generator mav also be fitted to the side wall of the unit. Spread the paraformaldehyde evenly over the Decontamination procedure heating surface of the frying pan.
·. Wamiitg! Cautionr This procedure should be performed by The auto-ignitio11 temperature of paraformal qualified technicians only. dehydeis 572oF (300°C).
1. Surface-disinfect the inside of the viewscreen 7. Place a hot plate, beaker of water, and and all other surfaces on the viewscreen temperature and humidity indicators on the assembly. cabinet work trily. 2. Calculate the total volume of the cabinet by 8. Close the opening to the work area with multiplying the height, width, and depth. heavy gauge plastic .film and tape. Close all 3. Multiply the total volume of the cabinet by possible leak areas, such as the exit of 0.3 g/ff of space to determine l~e gram electrical cords, the a.rea around the weight of paraformaldehyde required. viewscreen, the suction slots, and the jcmcti.on 4. If the cabinet is equipped with an exhaust of the plastic film and cabinet. duct, this duct must be gas tight. This may 9. Determine the temperature and humidity be accomplished at the terminal end of the inside the cabinet. duct, or if present, at the damper located near 10. The temperature should be 21.1 cc (70°F) or the cabinet. If the exhaust duct is more than higher, and humidity should be 60 to 85%. 10 feet long, additional parafonnaldehyde Use the hot plate to heat the beaker of water may be needed to compensate for the until the desired temperature and humidity increased volume. If the cabinet exhausts are achieved. into a recirculating building exhaust system, disconnect the cabinet from the building 11. Plug the cord of the electric frying pan into an outlet not installed on the cabinet, if the sysle.rn and fomt a gas tight seal. {Pb~stic filrn cabinet is not outfitted with a Formalin and tape may be used.) v0porizer. 5. If the cabinet exhaust air is discharged into the room, tape a plastic cover over the 12. After 25%, of the paraformaldehyde has exhaust port. A flexible hose can be applied depolymerized, tum on the cabinet blower(s) to an opening in the plastic cover to exhaust for 10 to 15 seconds. Repeat after 50%, 75% formaldehvde gas folhwing decontam and 100% of the paraiomlaldehyde has ination. Ho\VC\-'er, the end of the flexible hose depo!yrnerized. rnust be sealed with pb.stic film and tc.1pe !Vote- \!\lith the viezuscreen sash closed arul during decontamination. This flexible hose the blozvet on, a high flozv condition -will can be directed into the roorn, another cabjnet raast likely exist. In this 1node the audio or hood exhausts ystem, provided the porlio11 of the ailflow a/ann is disabled. Y1w exhaust is not recin..lllated. It m_ay also be unit is safe to run in this fashion.
16 13. Disconnect the hot plate and frying pan from Using a blender the electrical outlets. Homogenizing cultures with a blender can 14. Allow the cabinet to stand for a minimum of create an en01~nous aerosol load. so special hvo hours, preferably overnight. precautions must be taken. It is essential to " 15. If it is :not possible to attach a flexible hose, decontaminate surfaces and carry out an air purge both before and after the use of the blender. Do add the same amount of N~HC03 as of paraformaldehyde to the frying pan. Tum on not perform other research activities or leave your the frying pan and the cabinet blower until arms in the cabinet while the blender is in operation. And wait at least five minutes after the the NH4HC03 has dissipated. blender has come to a complete stop before you 16. Let the cabinet stand for at least one hour open its cover. before opening the seals. The air safety barrier could possibly be penetrated by the high concentration of contam Using Ancillary Equipment inated particles if the blender were opened during The rule to keep in mind is that the more or just after operation. In that case, the equipment is in the cabinet, the greater will be the surrotmding laboratory would very likely be air turbulence it causes. The turbulence resulting contam ina ted. from equipment and materials can disrupt the designed airflow and reduce the effectiveness of Using a centrifuge the cabinet. When you use equipment which Srna!! clinical centrifuges can also create severe rotates, vibrates or heats, be sure to place it at the turbulence because of their rotating action. They rear of the work area if possible. This will disrupt the airflow within the cabinet 17 delicate and the filter media should never be The life of a filter is determined by how it is touched. used and how often. Under normal laboratory Proven efficiency of all HEPA filters used in conditions, you can expect at least five years ~f Baker cabinets is 99.99% for particles 0.3 microns use. However, misuse or a heavy dust load iJl diameter. The 0.3 micron particle is used as the within the cabinet will shorten any filter's lifefime. basis for filter definition because theoretical Bunsen burners and misuse of chemicals will also studies have shown that filtration efficiency shorten the useful life. should be at a minimum for particles of this diameter, with efficiency increasing for particles Operating procedures either larger or smaller. (Model SG400ULT comes After many years of seeing our equipment used with ULP A, Ultra Low Penetration Air, filters and misused, we have made up this list of which have a proven efficiency of 99.999% for particles 0.12 microns in diameter.) Experiments suggestions: with various viruses and microbial agents have • Store equipment and supplies outside of the proven the effectiveness of HEPA (and ULP A) cabinet. filters. Contact Baker for more information. • Always tum on the blower. It must be pointed out that HEPA (and liLPA) • Set the view screen at the proper height. filters are not intended to filter gasses or vapors. Since most Baker cabinets are partially • When possible, use pipetting aids. recirculating, there will be gaseous bttildup to the • Avoid use of zm open flame within the point of equilibrium. Before any chemicals are cabinet unless the use has been specifically used in the cabinet it is necessary to consider approved by a safety professional. these points: , • Always keep the air intake grills clear and 1. Are these chemicals, either singly or in unobstructed. combination, able to attack filter components? • Only use toxic, explosive or flammable 2. Are these chemicals potentially toxic to the substances if a safety professional has operator? Is there any combination of two or approved them for work in your cabinet. more which could be toxic? If the cabinet is being used correctly and only the operator's • The control system will adjust automatically hands and arms are inside the work area, for small changes in the load on the blower: then toxicity or irritation could occur through Adjustments for larger changes should only skin penetration. A proper evaluation of be made by a qualified technician. Don't toxicity must deal not only with onetime change the cabinet or blower speed unless the exposure, but also with the effect of many change is required by a decrease in measured small exposures over a period of time. air velocity. 1,Alu,..,.,..'k- ~"~tih ,..,..,..,1~, 1"""'- ,...,.,.. Trln..-io-r::'l-!-a_r-icl. ::'lcre>nh::. 3. Are these cllemicals explosive or flammable? • • J..~, ¥> J. I \ •.JJ.UJ In cases vvhere dternical carcinogens! mutagens If b'1e operators are well trained and use good or teratogens are to be used, the risks should be common sense 'ivhen operating your cabinet, you carefulJv \veis;hed in choosing a cabinet \Vhere should have very fe'l>" problems. the exh:1lJSt effluent contains~ contaminant it may need treatment. 18 IV- ON-SITE CHECKS AND MAINTENANCE PROCEDURES We recommend that the following checks be 2. Usc one of the following methods: performed before initial use, after reloca~ion, and I) Primary method using a Direct Instrument Measurement after each filter change. They should also be Device (DE'vl) sealed lo the fronl access opening. Taking the av carried out at regular intervals, usually six erage of five reading, divided by the front access opening months or one year, as specified by an i.rldustrial square footage lo calculate inflow velocity. Sash areas arc noted below. The ca lculated inflow velocity required is I 00 to 1 10 hygienist, safety officer or other qualified person. FPM. The tests described below meet recommended 2) Secondary method using a thermal anemometer mounted minimum requirements. They must be on a ring stand S inches above the exhaust filler, lake reading on performed by an experienced technic.km using a grid as shown on the cabinet air flow data plate. Average proper procedures and instruments. Our these reading and multiply lhc average by the open area of the representatives can tell you about other tests exhaust collar installed over the exhaust filter. SG400 1.72 which you may consider desirable. square feel and SG600 2.37 square feel. Divide thi s value (CFM) by the sash open area lo determine the inflow velocity. As reported earlier in this manual, each individ The calculated inflow velocities required are, SG400 125 to 135 ual cabinet made by The Baker Company is care FPM and SG 600 liS to 125 FPM fully tested before it leaves the factory. Your copy of the test report, which you will find a!' the back Sash areas 8 Inch sash 10 loch sash of this manual, gives the factory test results for SG 400 2.56 ft2 3.19 ft 2 2 2 your own SteriiGARD~' II cabinet. Use it as your SG600 3.89 ft 4.86 ft record of the original testing, and as your guide to 3. Using the same instrument mounted on a ring stand within the testing in the future. To gain many years of satis work area, take readings with the anemometer probe set in factory service, please be sure that your mainte- plane of the bouom of the sash with the supply air diffuser in .) nance personnel come as close as possible to du stalled. TI1e location and average of these readings should be plicating these original test figures. the same as shown in the factory test report and the table below. Your test procedures should be identical to ours Uniform Downflow Zone Downflow so that comparing test results will have meaning. Sin Sash lOin Sash Please correspond directly with us to request SG400 60 to 70 FPM Zone I 62-72 FJ>M 60-70 FP.\1 detailed procedures for your particular cabinet Zone 2 110-120 FPM 110-120 FPM model. Alternate testing procedures can be found SG600 60 to 70 FPM*Zone I 60-70 FPM 62-72 FPi'vl in the NSF International Standard No. 49. Zone 2 105-11 5 FPM 100-110 FPM *SG600 10" sash is only zone downflow. The Airflow Balance Warning! This procedure should be performed by 4. Alter you have compared your figures with qualified technicians only. those origin.aUy recorded at the factory, make whatever adjttstments may be necessary. The airflow balance which is set at the factory provides 5. As the HEPA (High Efficiency Particulate your unit with air volume and vclocit y control to minimize Air) filters load up with particulates, airflow leakage of airborne contamination either in or out of the work wiJI be maintained automatically, at least area. until the filter resistance i:rtcreases 50 percent In order lo duplicate as closely as possible the aiflow char or more. When airflow eventually diminishes, acteristics described in lhe original factory lest report. please an alarm wi.ll sound. fiNe explained the alarm follow these steps: function at the beginning of Section 3.) You I. Adjust the viewscrecn to its connerct 8-inch operating bcigbl. If the viewscreen is scllo any other bcighl an alarm will have to increase the blower speed in or will sound. der to maintain the original volume of recir- 19 culating air. There is a speed control located 4. Holding the photometer probe about one in the light canopy. inch from the filter face with the diffuser Tum it clockwise until you have the removed on the downstream side, scan the desired airflow. If the airflow cannot be entire surface area and perimeter (filter maintained, it will be necessary to replace gasket frame area) in slightly overlapping the HEP A filters. (See "Replacing the HEPA strokes at a traverse rate of not more than ten Filters' later in this section.) feet per minute. Repeat at 90 degrees to the first scan pattern. 5. Eliminate leaks in the gasket frame area by . WARNING! re-tightening the filter gasket. When the light canopy is lowered, some 6. An HEPA filter i.s considered acceptable electrical parts are exposed. Do not perfonn when all significant leaks have been sealed. ,:his work unless you are a trained electrician or. electronic teclmician; , Airflow Smoke Pattern Test Filter Media and Seal Leak Tests To check for the direction of air movement, use a smoke generator and trace along the front access When preparing your cabinet for use after opening on the inside of the cabinet. Observe that shipment, and then at prescribed intervals no smoke is escaping from the work area. throughout its working life, you will need to In order to be sure that room air is not entering verify that the filters have maintained their the work area, trace along the outside of the front integrity. This is done by probe-testing the filter access opening. Observe that no smoke faces and seals. penetrates farther into the cabinet than the front The equipment you will need includes: perforated grille. • An aerosol photometer. The instmment should sample air at a flow rate of 1 CFM. Cabinet Integrity Test • A D.O.!'. generator with Laskin nozzle(s). Liquid dioctylphthalate (D.O.P.), The purpose of this test is to detennine that the dioctylsebacate or a comparable substance is cabinet welds and gasketed seams are free of aerosolized by flowing through the nozzle(s). detectable leaks. When generated with Laskin type nozzle(s), 1. Decontaminate the cabinet if it has been used. the mean droplet size of the aerosol is 99 2. Seal off the exhaust filter port. This may be percent less than 0.3 microns. done by instal1ing a gasketed panel over the Here is the procedure: opening using the stods on the filter box 1.. flange. An alternative is to tape a piece of it according to the manufach1rer's cardboard or plastic over the opening. instructions. 3. Disconnect the electricity and then remove 2. Position the D,O.P. generator so as to the two nuts holding the canopy clips at the introduce air generated srnoke into the area top of the canopy. Let the canopv pivot upstream of the filter. down. Disconi1ect the electrical plugs inside on e;Kh end. Disconnect the restraints and 1. 1Vfeasure the tlpstrearn concentration of hlnges. Lift the canopy up 2Lrtd off. D.O.P. 4. Lm,\.rer the vieVv'Screen untiJ lt is cornp ietely closed. WARNING! 5. Remove the top viewscreen tracks by Do not do this unless the unit has been removing the tvvo cap nuts at the top of each properly decontaminated. track, then puJJ.ing upward on each track. 20 6. Tape a sheet of plastic or other material Ultraviolet (Germicidal) Lamp around the viewscreen and the lower tracks. As reported in other sections of this manual, UV 7. Attach compressed air to the drain valve and germicidal lamps lose their effectiveness over time pressurize the cabinet to 2'' water column. and should be replaced when their intensity at the This pressure can be maintained by flowing work surface drops below 40 microwatts p~r compressed air if there are leaks in the taped square centimeter. area. J£ your cabinet has a germicidal lamp, 8. Apply liquid leak detector along all welds, frequently measure its intensity at the work gaskets, penetrations and seals on the surface with an ultraviolet light meter. The exterior surfaces of all cabinet p lenurns. A appearance does not indicate UV effectiveness. leak will be revealed by the appearance of bubbles. Make sure not to miss large leaks Check the HEPA Filters Regularly which may blow the leak detector solution away without creating bubbles. Changes in areas surrounding the laboratory may produce unexpected dust or other conditions 9. Repair all leaks until no further bubbles which affect the filters. To maintain filter integrity appear. and good cabinet operation, check the air pressure 10. Remove the compressed air lines, making monitor (or magnehelic gauge) periodically. If the sure that the drain valve is closed. Remove tmit consistently operates ne 21 advisable to seal the contaminated side of the properly seated on the flange. Replace the filter filter by taping a plastic sheet or cardboard over clamp assemblies and screw the stainless steel the face before removal. This should minimize studs finger-tight. Tighten the studs unifom1ly the number of particles shaken loose from the and moderately, a few threads at a time, until the filter. Once removed, the filter should filter gasket has been compressed about 20 immediately be sealed in a chemical hazard bag percent. Do not overtighten. Then replace the and then disposed of safely in accordance with exhaust filter access panel securely and make the environmental regulations. leak test described earlier in this chapter. After filter replacement has been camp leted, the cabinet and the room should be cleaned and decontaminated in a manner consistent with the Changing the Supply Filter nature of the hazardous material. The cleaning Move the viewscreen to its fully-closed position. materials, along with the protective gear and Remove the canopy retaining wing nuts and clothing, should be properly disposed of. swing the canopy down to its open restraint HEPA filters are very easily damaged, and you position. Loosen the retaining hardware (cap will want to use great care in handling so as to nuts) at top backside of each upper track. Pull the avoid injury to the filter mectia and gasket upper tracks out to the side at the top to clear the surfaces. When installing the new filters, it is a slots and pull them up and out of the lower tracks good idea to tape a p ieee of cardboard over the to remove them. filter media to give protection against dropped Remove the front access panel on the top section wrenches or misdirected fingers. Of course, you'll of the cabinet, as well as the inner supply filter need to make sure that the cardboard is removed access panel. Loosen and remove the supply filter before the access panels are re-insta.lled. Inspect clamps and carefully lift the dirty filter into a heat the filters carefully before and after installation. A sealable polyethylene bag for disposaL broken or damaged filter is worthless. Use a replacement filter of the correct type. Put a light coat of silicone grease on the face of the replacement filter gasket. Clean the sealing flange Changing the Exhaust Filter thoroughly. Then slide the new filter into place Move the viewscreen to its fully-closed position. and make sure that its correctly seated on the Remove the canopy retaining wing nuts and flange. swing the canopy down to its open restraint Replace the filter clamp assemblies and screw position. Loosen the retaining hardware (cap the stainless sh1ds finger-tight. The filter gasket nuts) at top backside of each upper track. Pull the should be compressed about 20 percent. Finally, upper tracks out to the side at the top to clear the replace both the access panels securely and then slots and pull them up and out of the lower tracks the viewscreen frame/light canopy assembly. to ren tove the11 t. vVhen filters are replaced, the airflow must be Remove the front access panel on the top section balanced. Thorough filter and cabinet leak tests of the unit, as well as the smaller inner exhaust must be made by qualified personnel. access panel. Loosen and remove the exhaust filter damps and remove the filter thrmJgh the front opening into a heat-sealable polyethylene Adjustment of the Balancing Damper bag for disposal. If the unit is not connected to an exhaust system, the exhaust filter nl_ay be Filters for the cabinet should be selected as a rerno\'ed from the top of the w1it pair so that their resistance ~Nill be as close as Prepr:re the new fHter by puttirtg a Light coat of reasonably possible. Resistance of HEPA filters silicone grease on the face of the gasket. Clean the may vary considerabl.Y from one to another, so a sealing flange thoroughly. Then carefully slide damper has been installed on vour cabinet's the new filter into place and make sure that it is exhaust to maintain the proper balance between 22 exhaust and recirculating air. This balance is When a smoke test indicates that there is air critical because too much positive or negative flowing from the interior of your cabinet into pressure in the work area could mean an outflow the surrounding room - of pathogens or an inilow of room contamination. 1. Make sure that the shipping cover is The balancing damper adjusts the volume of the removed from the exhaust filter and that no exhaust air so as to maintain the proper supply other objects are blocking the airflow. and calculated intake air velocities. The damper is 2_ If your cabinet is cmmected to an in-house preset at the factory and should not be changed exhaust, make sure that there is adequate unless the proper airflow balance carmot be exhaust suction and the system is not achieved after new filters are installed. On those producing back pressure. Also, be sure the occasions when adjustment becomes necessary, dampers are open. Re-balance the exhaust adjustments can be performed by a damper system to handle an adequate volume of air quadrant located on the top of the unit. and static pressure (suction). Consult with building maintenance people. The procedure is as follows: 3. The exhaust filter mav be loaded with dirt if 1. Loosen the quadrant wing nut. the urtit has been in service for some time. Decontaminate, and replace all filters. 2. To decrease exhaust airflow, move the quadrant arm towards the front of the unit 4. There may be high cross-drafts in the room (clockwise rotation). which are causing the outflow of smoke. Check the airflow balance, following the 3. To increase exhaust airflow, move the procedure recommended earlier in this quadrant arm towards the rear of the unit chapter. Eliminate the source of the cross (cotmter-clockwise rotation). draft. 4. When final adjustments have been achieved, make sure to tighten the wing nut on the damper quadrant. When there is low airflow within the work area and through the exhaust filter - 5. If, after adjusting the darnper, both supply and exhaust are high or low, adjust the speed 1. Check the incoming line voltage. Low control. voltage may cause the blower to operate at a slower-than-designed speed. Although this should be corrected in the building's electrical system, you may be able to compensate by adjusting the blower speed control clockwise Troubleshooting until proper velocity is reached. The control Here are some suggestions based on our is located on the left side of the light canopy. experience with the use and misuse of biological 2. The reduced airflow may be caused by safety cabinets. damaged or leaking filters. Decontaminate the unit and replace the filters at once. DANGER! Whene71er the potentially contaminated areas If there is no air flow within the work area - of your cabinet 1nust be· entered, 1nake s;.tre 1 1. If the lights and electricaJ outlets also fail to that the unit is first decontaminated by use of j operate, make sure the Llnit is plugged into a appropriate 1nethods. 1 dedicated eJectricaJ outlet (grounded 20 amp, 115 Volt, 60 Bz). Also make sure that the blower switdl is turned on. The yellow indicator above the button should be lighted. 2. If the lights are working, then tum the blower When there is uneven fan operation, or noise switch off and let the cabinet rest for ten from the motoriblower assembly- minutes. When the time has passed, tum the l. lt may be necessary to decontaminate the blower switch on again. If the blower starts, cabinet. you kndw there has been overheating of the 2. Look for loose objects in the fan cage. blower motor. Also check the wiring connections inside the right end of the light 3. Check to see if the fan wheel is contacting the canopy which connect the canopy to the blower housing. cabinet. Be sure the connections are pushed 4. Remove wing nuts and drop canopy to open together. (full restraint) position. Check the multi-pin 3. If these solutions do not correct the problem, connectors at each end to be sure they are or if the blower failed to start after the rest securely engaged. period, then either the speed control, blower 5. Another possible source of the problem is the motor or capacitor is defective. A qualified speed control. electrician can find out if the speed control is defective by bypassing it, using the wiring diagram in the Appendix of this manual as a When the viewscreen open alarm is sounding - guide. If there is a noise problem, it may be 1. The viewscreen alarm should be silent in only caused by faulty motor bearings. two positions. The first silent positior, is when the viewscreen is at its proper operating height (8" standard). The second If one (or both) of the electrical outlets does not silent position is when the viewscreen is in a function- closed position. The viewscreen alarm is 1. Be sure the outlets on/ off switch has been located in the light canopy and is de-activated pressed. The yellow indicator above the when it senses hidden magnets attached to switd1 should be on. the viewscreen frame. 2. Both outlets are equipped with ground fault 2. If the viewscreen is in an incorrect position, interrupters. If the button on the G. F.!. outlet the alarm will sound. The indicator above has popped out, press it in to reset. the alarm reset button will flash. You may 3. The outlets are also protected by a self cancel the audible alarm for five minutes by resetting thermal circuit breaker. Check this pressing the alarm reset button. The breaker. indicator will continue to flash until the viewscreen is set in the correct position. After five minutes, the alarm will sound If the ultraviolet light does not work - again. You may press the alarm reset button 1. Be sure t_.1-lat t.l-te l,r]e-.;,vscreen is completely a gaul. closed, and the fluorescent light is off. 2. Check the ultraviolet bulb and lamp sockets If the fluorescent light does not work - for good electrical connections. l. The blower switch should be turned on, and 3. Change the ultraviolet light bulb. the yellow indicator above the switch should 4. Hcn/e a qualified electrician check the vviring be lighted and baUast for continuity'. The vvirin,g can be Rernove \-Vi.ng nuts and drop G1nopy to upen traced to the source of a break If none of the (full restraint) position. Check the multi-pin abcYve is effective, the ballast rnay need com1ectors at each end to be sure they are replacing. securely engaged. If the larnp flickers and can be corrected by vigorous rubbing of t11e bulb, there is probably an in1proper ground. 24 The wiring can be traced to the source of a break. If the air pressure monitor or magnehelic gauge has high or low readings- A ru.gher reacting than .09 to .12 inches water column could be an indication of the following: 1. Blocked or partially obshucted perforated grilles (front and/ or rear). 2. Towels or wipes have blocked the towel guard on either the back or sides of the unit. 3. The viewscreen is in the closed position and the in-house exhaust fan is still pulling air though the cabinet. 4. Incorrect air flows For any of the above conditions, always check for proper air flows first. A lower reading than .09 to .12 inches water column could be an indication of the following: 1. Partially or totally blocked filters. 2. Incorrect air flows. 3. Perforated grilles or worksurface has been removed. For any of the above conditions, always check for proper air flows first. 25 ""''00 a REV. I O(SCRIP~ AP?"O. i CA tt DEDICATED 20A rh CHASSIS GROUND- l&""T SIDEWALL RJM lllt1LIW SOCKET ONLY 'E I - txxnO -·:.;; SRC I,,.,..,. INCOMING )ill.Q._ W OTB-1 OTB-i POWER o ,_/ NEUTRAL NEMA 5-20P PLUG 12 5 j~~CA- '"-HOT JI--~'AJ1 '!,; OTB, LEFT SIDEWALL OTB-2 ---s-J ' Ph PI-!!)'m ~ 12 PI,J1, CANOPY RIGHT J1-~~~1-3 w 16 POWER CONNECTOR 20272 TB, CANOPY TERMIN/>1. J1-7co;:: 14 J _ 14 DSC: BLOCKS ~ 1,o1 5 P1-7 , _ w w w Q]lL--;:-;-;~;;;;;:<(.:::C:::::'f'A8VACx2 TRAF~"W TB I,2'W so-~ '·,r~ P.T.c. rJS£ L/'~"" Q 12D ,r. l<> 60 MEMBRANE SWITCH g~~~ ~J~ ~ti ~ ~ii ~~ l CIRCUIT BOARD 34699 11'!!3 ; ~ -"1 "' 1-W~ ~ 101 ~c= H~ ;1- -o11 ~~-" , MEMBRANE SWITCH 34615 65 I:~';: "-'"_; Wl• I~ io MEMBRANE SWITCH .J '1&1"" 1~c .•. ovMv• 1 -, "":; 1 P.c. BOARD 0 11 SASH SENSOR 34609 12 DESCR!PTION 70 SA$~ CLOSED SENSOR i '""" 1l!lf3 ,I tne BAKER COMPANY, inc. DArt: 6@96 SA.'WORO, I.I~N( 0...07:0 SENSOR I ~.;~~~ 11 'ca< i Tl£LADOFR SCHEMATIC 75 "" SteriiGARD SG400 3 TIER STANDARD/ULTRA LEGE,(!O ~k DENOTES WIRE NO. 54, BLUE.. 20 A..W.G. LO ALL WIRE 16 A.W.G. UNlESS OTHERWISE NOTED iB-3,4 DENOTES CANOPY TERMINAL BLOCK, TERMif>IALS 3 OR 4 Wiring Diagram- SG400 Models 26 CHASSIS GROUND- LEFT SIDEWALL R£'1\S!QN REV. I DESCR!f'110H I'Pf>'O, DATE I RJH M"~ .... """""-· SRC 5.'14/911 ~-- NEMA 5-20P PLUG & CORD OTB, LEFT S1DEWALL Pi.J 1. CANOPY RIGHT POWER CONNECTOR 20272 TS, CANOPY TERMINAL BlOCKS 3Y'·'/ :) STEDNOLT SPEED CONTOLL£R 304227 QUAD S.S. RELAY 1855157 ~OTOR 31679 BLOWER 11432 POTENTIOMmR 30305 TB-1,2 FLUORESCENT BALLAST 34150 I FlUOR. LAMPS- F32T8 17927 LAMP SOCKETS 34455 30 l_:j U.V. 8Ml.AST 34155 U.V. LAMP- G6416 18025 35 U.V SOCKElS 20281 U.V SOCKEJS 20283 P2,J2, CANOPY LEFT 40 POWER CONNECTOR 20272 GFI OUTLEl 34921 STANDARD OUTLET 18231 45 SElf-RESET BREAKER 34331 TB-1.2 P.T.C. FUSE JS048 PTC FUSE TRANSFORMER 34326 .32 A 120VAC"' 12DVAC NEUTRAL GNO HOT 55 GH AlR PRESSURE oo MONITOR CIRCUIT i 00~~ 00 BOARD (OPTIONAL) 34697 gg~ 60 MEMBRANE SWITCH lgg~ CIRCUIT BOARD 34699 1!'!3 00~ oo.. ~ MEMBRANE SWITCH 34615 oo"' MEMBRANE SWITCH tiS A.P.M. P.C. t:JOM:U ! REED SWITCH 34609 DESCRiP'!ON JW:£11 110. 70 ~ ORAwt< • the BAKER COMPANY, inc. Wiring Diagram- SG600 Models '")7"7 .:.. ./ DimensJona. 1 Dravvm.g,. Model1 SG4005TD 28 , , I ' ' ! :::- ! ,' ' ' :n ,, i ' '" Dimensional Drawing, Model SG600STD ( ( 5 -~~M >, ~;~~ij S;ii~a < :J! ~------1 ' Dimensional Drawing, Model SG400ULT 30 I I I ------~---~ Dimensional Drawing, Model SG600ULT 31 REPLACEMENT PARTS LIST STERILGARD®II MODELS SG400STD & SG600STD VERTICAL LAMINAR FLOW CABINET Parl:Name SG400STIJ SG600STD Pari: Numbe1·s Part Numbc,-s BOM#1855199 BOM #1865199 Magnehelic Gauge 20776 20776 Capacitor 11557 11558 Circuit Breaker- SA 34331 34331 Exhaust l-IEP A Filter 1 'iS)( IVY tJ- 28322 36262 Fluorescent Ballast 34160 34160 Fluorescent Lamp 17927 17927 Ground Fault Interrupter with Indicator Light 34921 34921 Lamp !-!older, "Butt-On" Type 34465 34465 ' Magnetic Sensor 34609 I 34609 ! \lembrane Switch Controller 34699 34699 _c I 304227 J' Motor /Blower Controller 304227 Relay, Solid State I 33985 33985 Sash Balance - Left Piston I 36475 '. j 3i,<-\l R Sash BaliU1ce - Right Piston 36476 J - 31A! /L-- Supply Blower 11429 I 11432 Supply HEP A Filter ~~Ji:_ l?i'YY?Y3 28302 28303 Supply Motor 31676 31679 Switch, Touch-Type I 34615 34615 Transformer 34326 I 34326 ' Ultraviolet Lamp 18023 18025 I Ultraviolet Lamp Ballast ! 3416.5 34165 Ultra\'iolet Lamp Socket I 18088 ! 20281 & 20283 \""indo-w Assemblv I 1851703 I 1861692B ' I \Nindc.YV\' VV.lper 1855095 1865095 REPLACEMENT PARTS LIST STERILGARD@II MODELS SG400UL T & SG600UL T VERTICAL LAMINAR FLOW CABINET Part Name SG400ULT SG600ULT I Pa17 Numbers Part Numbers BOM SG400ULT#194 BOM SG600ULT#194 Air Pressure Monitor 34697 34697 Capacitor 11557 11558 ' Circuit Breaker - SA 34331 34331 Exhaust Filter 37206 (ULPA) 36262 (HEP A) Fluorescent Ballast 34160 34160 Fluorescent Lamp 17927 17927 Ground Fault Interrupter with Indicator Light 34921 34921 Lamp Holder, "Butt-On" Type 34465 }'1,465 Magnetic Sensor I 34609 34609 ' Membrane Switch Controller 34699 34699 Motor /Blower Controller 304227 304227 Relay, Solid State 33985 33985 Sash Balance -Left Piston 36475 J,b fi'b 3467'8 Sash Balance - Right Piston 3M76 1.(,'(11 31Mi'7 " Supply Blower 11429 '1,'7• 11432 4,"1 Supply Filter 37173 (ULPA) 28303 Supply Motor 31676 31679 Switch, Touch-Type 34615 34615 ':L1 '2.'),(. Transfonner 3432G I o.J .._._.._,..' Ultraviolet Lamp 18023 18025 Ultraviolet Lamp Ballast 34160 34165 I Ultraviolet Lamp Socket I 18088 20281 & 20283 ,[1 Jl I Window Assernblv 1851703 . 1>161 0'en~ \·Q" I fiJi' /ViY ' , ."-v ~· I '/.h" _., .. ! 0 36"09"' I \Nindovv Vv'iper I 1055095 i' l(, ~I ~ NOTE: When ordering replacement parts, please furnish the serial mm1ber of the unit, as well as the model number. 0 c\ (;·'/,..,,/ '- SY ._) t;; twt:Jt, L.:_ '- I ,• J! .,L.. r f'-1 0 I'-''"- 0"1 f /_ f c ' /c;J.:S''' " Ji..--1 C 'TV Q_j/c:;)J:J:.f?r<.:- The Baker Company, Inc., expressly represents and warrants all goods (a) to be as specified (and described) in The Baker Company catalogs and literature, and (b) to be free under normal use, service and testing (all as described in The Baker Company catalogs and literature) from defects in material and workmanship for a period of thirty six months from the invoice date. The exclusive remedy for any breach or violation of this warranty is as follows: The Baker Company, Inc., will F.O.B. Sanford, Maine, furnish without charge repairs to or replacement of the parts or equipment which proved defective in material or workmanship. No claim may be made for any incidental or consequential damages. THIS WARRANTY lS EXPRESSLY IN LIEU OF ALL OTHER WARRA1\JTJES, EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR ! FITNESS FOR A PARTICULAR PURPOSE UNLESS OTHERWISE AGREED IN WRITING SIGNED BY THE BAKER COMPANY. (THE BAKER COMPANY SHALL NOT BE RESPONSIBLE FOR ANY IMPROPER USE, INSTALLATION, SERVICE OR TESTING OF THE GOODS.) 34 THE BAKER COMPANY TI1e Baker Company, Inc. P.O. Drawer E Sanford, Maine 04073 Tel: (207) 324-8773 I 1-800-992-2537 Fc~x: (207) 324-3869 Copyright©l998 by TI1e Baker Company. All rights reserved. Artisan Technology Group is an independent supplier of quality pre-owned equipment Gold-standard solutions We buy equipment Learn more! Extend the life of your critical industrial, Planning to upgrade your current Visit us at artisantg.com for more info commercial, and military systems with our equipment? Have surplus equipment taking on price quotes, drivers, technical superior service and support. up shelf space? We'll give it a new home. specifications, manuals, and documentation. Artisan Scientific Corporation dba Artisan Technology Group is not an affiliate, representative, or authorized distributor for any manufacturer listed herein. We're here to make your life easier. How can we help you today? (217) 352-9330 I [email protected] I artisantg.com