DOCSLIB.ORG

Blood Collection, Processing and Quality Control Adapted Procedures for Kality

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Blood Collection, Processing and Quality Control Adapted Procedures for Kality Blood Collection, Processing and Quality Control Adapted procedures for Kality Tsetse flies are fed on quality-tested fresh defibrinated blood, which has been stored in a frozen condition (Wetzel and Luger 1978). The following procedures describe the collection of animal blood in the abattoir, radiation with gamma-rays (decontamination), preservation and storage in deep freeze, quality control assurance and processing of the blood into diet for feeding tsetse flies. The procedures aim to provide nutrition at a constant quality to mass-reared fly colonies maintained over a long period for field programmes. The following is the standard procedure specifically created for the insectary at Kaliti. Before collecting the blood, all the equipment that will be in contact with blood must be thoroughly cleaned and sterilized. During the blood collection in the abattoir, the fibrin is removed by mechanically stirring the blood or by adding anticoagulants to stop the blood from clotting. If slaughtering takes place on the floor, the blood collection equipment used is selected accordingly. After collection, a sample of blood from the batch is checked for microbial contamination, and bioassayed for its nutritive value using female adult flies. All blood fed to flies must first be decontaminated, for example by treatment with radiation. Blood will then be stored in frozen conditions (-20 ⁰C) until the results from the quality control tests are ready. If results show no bacterial contamination and good nutritional value, blood will be thawed and prepared to be used. Table of contents Blood Collection, Processing and Quality Control ..................................................................... 1 1. Sterilization of materials in heat sterilization oven or autoclave ........................................ 5 2. Blood Collection ................................................................................................................. 6 3. Labelling bottles and samples............................................................................................. 9 4. Deep freeze ....................................................................................................................... 12 5. Blood thawing................................................................................................................... 13 6. Sampling for 25-day feeding test (bioassay) .................................................................... 15 7. Sterilization by radiation .................................................................................................. 17 8. Microbial screening of the blood ...................................................................................... 19 9. 25-day feeding test: bioassay ............................................................................................ 21 10. Proportioning ................................................................................................................ 25 11. Sampling for microbial screening of the blood............................................................. 27 12. Use of the database ....................................................................................................... 29 Summary of blood processing procedures Before the collection day, the containers, stirring material and any other material that will be in contact with the blood must be sterilized in the oven over 24 hours before been used. (See Procedure 1) The collection will be done in the abattoir, using 25 litre stirring containers (See Procedure 2) that allow defibrination. Blood will be sieved and poured into 4L bottles. After driving to Kality, the 4L bottles will be labelled (See Procedure 3) and stored in the cold room (See Procedure 4). Blood now must be checked for microbial contamination, bioassayed for its nutritive value and proportioned into bottles with a manageable size to be irradiated and fed to tsetse flies. According to a programmed schedule, all the 4L bottles that have been collected on a same day will be thawed following the procedure described (See Procedure 5). Blood will be mixed into a 100L container, previously sterilized, to obtain one overall sample of the blood collected each day (See Procedure 6). This sample will be later used for the bioassay (also called 25 day feeding test) and will be composed of 25 vials labelled carefully (See Procedure 7). One single bioassay will be carried out for each collection day. After sampling for the bioassay, the blood in the 100L container is proportioned into small bottles, usually with a volume of 1L or 2L, (See Procedure 11) and labelled (See Procedure 3). The labelled bottles will be frozen until the result of the bioassay is available. During the proportioning, a sample of the blood of each bottle must be taken (See Procedure 11) and labelled (See Procedure 3). This sample must be kept together with its original bottle and will later be used for microbial screening. There are now two types of samples: the ones for the bioassay, composed by 25 vials, and the ones for the microbial screening, one Eppendorf for each bottle obtained after proportioning. The bioassay samples will be irradiated (See Procedure 8), after which, the 25 day feeding test will be conducted (See Procedure 10). Microbial contamination of the blood in the bioassay sample is assessed before starting the bioassay (See Procedure 9) to ensure that the blood in the sample is not contaminated If the quality factor of the blood is above 1, the frozen 2L bottles and its Eppendorf samples will be irradiated (See Procedure 8). Both the bottle and its sample will be irradiated in the same operation to ensure that the sample receives the same dose as the blood in the bottle. The last step is to check the bacterial contamination of the blood in the 2L bottles. For this purpose, we thaw the blood sampled in the Eppendorf tubes and conduct the microbial screening (See Procedure 9). If the number of colony forming units is below the agreed threshold, the blood in the bottle can then be marked as good quality. It will be kept in the cold store at -20°C until it is scheduled to be used for flies feeding. The whole procedures are summarized in the following flow chart 1. Sterilization of materials in heat sterilization oven or autoclave Scope: To prevent bacterial contamination, all equipment that will be in contact with blood during blood collection, blood sampling and blood proportion must be thoroughly cleaned and sterilized before being used. Equipment and Materials: The following equipment will be cleaned and sterilized: Materials to be used in blood collection: o 25 litre containers, including caps, side inlet and tap. o Paddle / stirrer o Hose o Funnel Materials to be used in blood sampling: o Eppendorf tubes (if not stored in proper sterile conditions) Materials to be used in blood proportion: o 2 litre bottles, including caps o Sieves The equipment will be sterilized either using a heat sterilizing oven or an autoclave. Procedure: 1. Clean the blood-collection equipment. 2. Depending on the dimensions of the equipment, select: heat sterilizing oven for large equipment: Sterilize the equipment by exposing it to dry heat (at 80°C for polyethylene (PE) for at least 3 hours, and glass at 120°C) autoclave for small equipment: 15 minutes at 103.45 kPa (15 psi). 3. When not in use, to minimize microbial contamination, store all sterilized equipment in autoclave bags or in an ultraviolet (UV) cabinet. Fig. 1. Sterilizing oven. 2. Blood Collection Before collection, make arrangements with the responsible authorities of the abattoir to ensure that the place, time and amount of blood to be collected are agreed upon. Alert the collection team (a minimum of three persons) to prepare for the work; note that some heavy lifting may be required. It is an advantage for the workers to have a good knowledge of basic biology and tsetse rearing procedures, and have the ability to handle mechanical equipment. In addition, the workers must be able to make sound judgments regarding the condition of animals (age and health) at the abattoir. The team must wear protective gear (white clothing, rubber boots and helmets). Before arrival at the abattoir, all equipment that will be in contact with blood must be thoroughly cleaned and sterilized. The equipment used to collect blood (Fig. 1 and 2) depends on the amount of blood obtained on one occasion. The frequency of collection depends on the size of the tsetse colony and the amount of space available to store frozen blood at -20°C. In general, during one collection operation, it is economical to obtain as much blood as possible because transport, human labour and quality-control measures become cheaper with increasing quantity. If the ambient temperature is high or the storage facility is a long way from the collection point, it may be necessary to cool the tank of blood. Using two or three collection sets, blood can be collected from all slaughtered animals even if the rate of slaughter is high, e.g. 100 animals in 5–6 hours. Freezing slows down, but does not stop completely, the degradation of blood. It has been demonstrated that blood kept frozen at -20°C for many years retains its nutritive characteristic. If animals are slaughtered on the floor, the blood collection equipment used is selected accordingly. Blood is collected from the cut neck of the animal in a bucket, and then immediately poured into a 25-l container for defibrination or into a container with anticoagulants. Scope:
Load more

Recommended publications
  • Supplement of Atmos
    Supplement of Atmos. Chem. Phys., 15, 2489–2518, 2015 http://www.atmos-chem-phys.net/15/2489/2015/ doi:10.5194/acp-15-2489-2015-supplement © Author(s) 2015. CC Attribution 3.0 License. Supplement of A comprehensive laboratory study on the immersion freezing behavior of illite NX particles: a comparison of 17 ice nucleation measurement techniques N. Hiranuma et al. Correspondence to: N. Hiranuma ([email protected]) 1 S1. Supplementary Methods 2 3 This supplementary information provides additional details for the measurement 4 techniques of immersion freezing of illite NX particles with S1.1. suspension techniques and 5 S1.2. dry-dispersed particle measurement techniques (both in alphabetical order as in Table 6 1). The discussions of measurement uncertainties of temperature and ns for each measurement 7 technique are also provided. We note that the uncertainty in frozen fraction (α) used in 8 calculating ns may not be adequate, since the sensitivity of Δα (an increase or a decrease in 9 frozen fraction) is much higher at high temperatures which unexceptionally coincide with a 10 low fraction of frozen illite NX. 11 12 S1.1. Suspension techniques 13 14 Bielefeld Ice Nucleation ARraY (BINARY) 15 16 The BINARY setup is an optical freezing apparatus that makes use of the change in 17 droplet brightness during freezing for the automated and simultaneous detection of ice 18 nucleation in 36 microliter-sized droplets. The droplets are positioned on a hydrophobic glass 19 slide that rests on top of a Peltier cooling stage (Linkam LTS 120). The 36 droplets are 20 separated from each other by a polydimethylsiloxane (PDMS) spacer in order to prevent a 21 Wegener-Bergeron-Findeisen process.
    [Show full text]
  • Biological and Chemical Safety Manual
    Biological and Chemical Safety Manual Fall, 2012 1 EMERGENCY INFORMATION (Fill out laboratory specific information and post outside of laboratory – copy to EHS dept.) Principal Investigator After -Hours Contact Information Lab Location Location Of Chemical Spill Kit Location Of Biological Spill Kit Location Of Fire Extinguisher Location Of Fire Alarm Location of Eye Wash Station Location of Safety Shower USD Environmental Health and Safety Chemical & Radiation Safety Kevin O’Kelley, Director of Environmental Health & Safety [email protected] , 605-677-6265 Institutional Review Board (IRB) Sandra Ellenbolt, Director of Human Subjects Protection [email protected] , 605-677-6067 Institutional Animal Care and Use Committee (IACUC) Peter Autenried, Director of Animal Resource Center [email protected] , 605-677-5174 Institutional Biosafety Committee (IBC) Victor Huber, Ph.D. [email protected] , 605-677-5163 Facilities Management Call Desk: 605-677-5341 (After hours, call USD Public Safety) USD Public Safety Office: 605-677-5342 Fire/Police/Emergency Medical Services Dispatch: 9-911 2 Preface A safe working and learning environment is an expectation of USD and is provided to all employees, students, and guests at the highest level reasonably possible. The University of South Dakota Biological and Chemical Safety Policy is designed as a reference for individual laboratories to provide a safe and productive work environment while complying with applicable federal and state rules and best practices. Each laboratory group should supplement this policy when necessary to insure health and safety of workers is not compromised. The policy is divided into Chemical Safety and Biological Safety sections. Radiation Safety is addressed in the University of South Dakota’s Radiation Safety Policy for Authorized Workers.
    [Show full text]
  • Pipettes & Syringes Departments 6 Editorial 34 Index
    LaboratoryEquipment.com November 2013 Featured New Products Compact Shaker is Lab Workhorse PRODUCTS AND TECHNOLOGY FOR LAB PROFESSIONALS Inside: Ī Life Science & Biomedical Ī Page 12 Thermometer Records Achieving a Good Clean, Min, Max Temperatures Inside and Out Laboratory washers, cleaning consumables and waste programs help keep the laboratory contamination-free. Ī Page 20 Pretreatment Reagent for Cytogenetic Assays Ī Page 28 Fume Hood Exceeds International Standards Ī Page 32 LLE3n_CV_digital.inddE3n_CV_digital.indd 1 110/30/20130/30/2013 33:36:59:36:59 PPMM Convenient Weighing Quintix® Make Your routine lab work easy with our revolutionary user interface. www.sartorius.com/quintix LLE3n_FPads.inddE3n_FPads.indd 2 110/24/20130/24/2013 44:06:37:06:37 PPMM Scan the code with your mobile device to go directly to SLAS2014.org Short Courses: January 18 - 19 Conference: January 20 - 22 Exhibition: January 19 - 21 Why Participate in SLAS2014, the Third Keynote Presenters Annual SLAS Conference and Exhibition? Eric J. Topol, M.D. Because it’s where more than 5,000 of the best laboratory science Director, ^ĐƌŝƉƉƐdƌĂŶƐůĂƟŽŶĂů and technology minds from across the globe come together to learn, ^ĐŝĞŶĐĞ/ŶƐƟƚƵƚĞ ĞdžƉůŽƌĞĂŶĚƐŚĂƌĞƚŚĞůĂƚĞƐƚŬŶŽǁͲŚŽǁĂŶĚŝŶŶŽǀĂƟŽŶŝŶůĂďŽƌĂƚŽƌLJ ŚŝĞĨĐĂĚĞŵŝĐKĸĐĞƌ͕ technology. Join your colleagues—innovative scientists, engineers, ^ĐƌŝƉƉƐ,ĞĂůƚŚ WƌŽĨĞƐƐŽƌŽĨ'ĞŶŽŵŝĐƐ͕ researchers and technologists from academic, government and dŚĞ^ĐƌŝƉƉƐZĞƐĞĂƌĐŚ/ŶƐƟƚƵƚĞ ĐŽŵŵĞƌĐŝĂůůĂďŽƌĂƚŽƌŝĞƐͶĂƚ^>^ϮϬϭϰĂŶĚĚŝƌĞĐƚůLJďĞŶĞĮƚĨƌŽŵϭϯϬ ŽƵƚƐƚĂŶĚŝŶŐƉŽĚŝƵŵƉƌĞƐĞŶƚĂƟŽŶƐĐŽŵƉƌŝƐŝŶŐϯϬƵŶŝƋƵĞƐĐŝĞŶƟĮĐ
    [Show full text]
  • Background: Laminar Flow Hoods – Crossflow Cabinet: Down Flow
    Laboratory Safety Cabinets – types and use Background: A fume hood or fume cupboard (FC) is a type of local ventilation device designed to limit exposure to hazardous or noxious fumes, vapours and dust. A fume hood is typically a large piece of equipment enclosing five sides of a work area, the bottom of which is most commonly located at a standing work height. Two main types exist, ducted and recirculating. The principle is the same for both types: air is drawn in from the front (open) side of the cabinet, and either expelled outside the building or made safe through filtration and fed back into the room.(the recirculating type is not recommended at the University due to testing issues however there are some specific uses where this is the preferred type i.e. lecture theatre). Some fume cupboards have wash down facilities, and some FC have filtering at the outlet. Laminar Flow Hoods – Crossflow Cabinet: Laminar flow cabinets (down flow or cross flow) are designed for product protection only and must never be used for work with infectious material. Their use should be limited to the preparation of sterile media, the assembly of sterile components into complete units (eg. membrane filters) or other similar operations. Laminar flow usually have a UV sterilisation capability for the work area prior to use and after use. A bunsen burner can be used in some cases. Down flow Cabinet - Class I Biosafety Cabinet: This cabinet is designed for personnel and environment protection. A protective barrier between the operator and the potential hazard is provided by creating a uniform inflow of air across the entire work opening of sufficient velocity to prevent egress of material from the cabinet work zone to the laboratory environment.
    [Show full text]
  • 2019-2020 Product Guide
    Guide to Esco Products and Services 2019-2020 Dedicated to delivering innovative solutions for the clinical, life sciences, research, industrial, laboratory, pharmaceutical, and IVF community 2 Guide to Esco Products and Services Table of Contents Corporate Profile ......................................................................................................................................................3 Tradition of Quality and Innovation..............................................................................................................4 Research and Development...............................................................................................................................8 Products and Applications..................................................................................................................................9 Sample Preparation Biological Safety Cabinets............................................................................................................................................10 Laminar Flow Clean Benches........................................................................................................................................11 Lab Animal Research Workstations............................................................................................................................12 Laboratory Centrifuges.........................................................................................................................................13 Sample Cultivation
    [Show full text]
  • Laboratory Catalog
    2014/15 Laboratory Catalog 2014/15 Catalog Laboratory Balances Software Titration Density Meters Refractometers pH Meters & Electrodes Thermal Analysis Pipettes Laboratory Catalog Automated Chemistry Service Analytical Excellence to Improve Customer Processes METTLER TOLEDO Contents General Introduction 4 – 23 LabX Software 16 – 19 Service 20 – 23 Balances 24 – 71 Applications and Accessories 72 – 97 Mass Comparators 98 – 103 Quantos – Automated Dosing 104 – 113 Moisture Analyzers 114 – 123 Pipettes 124 – 141 Titration 142 – 169 Density Meters & Refractometers 170 – 183 pH Meters & Electrodes 184 – 207 Autochem 208 – 219 Melting Point & Dropping Point 220 – 227 Thermal Analysis 228 – 238 You will find a detailed table of contents on the overview page of the respective chapter 3 Product Portfolio Innovative Products For a Wide Range of Applications METTLER TOLEDO's comprehensive product range provides users with flexibility and precision in every application plus outstanding quality and functionality. See the overview of our range below. Weighing Titration pH Measurement Thermal Analysis METTLER TOLEDO is con- The new generation From basic handhelds METTLER TOLEDO is the stantly changing the world of titration solutions to flexible two-channel technology leader in of balances. The Quantos focuses on ease of use meters, METTLER TOLEDO thermal analysis instru- automated dosing and and flexibility: Its unique offers a full spectrum of mentation. The innovative sample preparation solu- One Click™ user interface premium quality pH and product range includes tion and innovations keeps training times to a ion instruments. Seven- DSC, TGA, TMA and DMA, such as StatusLight, minimum and speeds up Excellence meters, for as well as an unmatched, StaticDetect and Smart- workflows.
    [Show full text]
  • Product Sampler: Hoodshoods && Wetwet Stationsstations
    Product Sampler: HoodsHoods && WetWet StationsStations © Copyright 2006 Terra Universal, Inc. Mini-Catalog Vol. 8, No. 8 Free-Standing See Catalog 105 Vertical Laminar Flow Stations Page 406 • Economical system provides a laminar flow of HEPA-filtered air to meet the most critical contamination requirements • Incorporates industry-leading 48 dBA blowers for quiet, low-vibration operation • All-stainless steel or powder-coated steel construction is cleanroom compatible and easy to clean • Side panels and optional motorized front sliding shield effectively control the laminar air stream to achieve the air speed you require • Fluorescent illuminators ensure easy viewing • Backpressure gauge with audible alarm helps monitor filter performance • Optional ionization equipment safely neutralizes static charges throughout the work area for effective ESD and particle control • Accommodates standard Terra work benches in many materials, as well as standard Terra wet processing stations (see page 30) Terra Universal’s Vertical Laminar Flow Station combines several of our most advanced products in a state-of-the-art work station that removes particulate air-borne contamination. Motorized Digital Control Panel Sliding Shield* • Filter/Fan Unit extends 3"-25" • Light below hood to • Backpressure alarm restrict access to work area and Fluorescent light improve system standard; UV efficiency. Rated sterilization bulbs for millions of available operations! Side Panels ensure effective laminar flow Strip shield access curtain * Modular Cleanroom Drawers*— select stainless steel or plastic Work Surfaces* available in many sizes and materials. Two-piece design isolates FFU vibration. IonBar™— Safe static control! See page 12 *System option NOTE: All prices and specifications are subject to change without notice.
    [Show full text]
  • The Compatible Solute Glycerol As a Photosynthetic Sink And
    THE COMPATIBLE SOLUTE GLYCEROL AS A PHOTOSYNTHETIC SINK AND ENERGY CARRIER IN FRESHWATER AND MARINE CHLAMYDOMONAS by TYSON ANDREW BURCH B.A., University of Colorado Boulder, 2007 A thesis submitted to the Faculty of the Graduate School of the University of Colorado in partial fulfillment of the requirement for the degree of Doctor of Philosophy Department of Ecology and Evolutionary Biology 2016 This thesis entitled: The compatible solute glycerol as a photosynthetic sink and energy carrier in freshwater and marine Chlamydomonas written by Tyson Andrew Burch has been approved for the Department of Ecology and Evolutionary Biology ________________________________________ Co-Chair: Professor Barbara Demmig-Adams ________________________________________ Co-Chair: Professor William W. Adams III ________________________________________ Professor Patrick Kociolek ________________________________________ Professor Brett Melbourne ________________________________________ Professor Ralph Jimenez Date_____________ The final copy of this thesis has been examined by the signatories, and we find that both the content and the form meet acceptable presentation standards of scholarly work in the above mentioned discipline. iii ABSTRACT Burch, Tyson Andrew (Ph.D., Ecology and Evolutionary Biology) The compatible solute glycerol as a photosynthetic sink and energy carrier in freshwater and marine Chlamydomonas Thesis directed by Professors Barbara Demmig-Adams and William W. Adams III The purpose of this thesis was to explore fundamental relationships between the photosynthetic production of energy carriers, the ceiling of photosynthetic capacity, and the regulation of photosynthesis in algae and how this insight may (i) serve to enhance the principal understanding of the limits of primary productivity and (ii) open up novel applications in the context of the generation of biomaterials and bioenergy from renewable resources.
    [Show full text]
  • “Ivf Chamber” in the Human Assisted Conception Laboratory
    TECHNICAL REPORT THE BENEFITS OF USING THE “IVF CHAMBER” IN THE HUMAN ASSISTED CONCEPTION LABORATORY INTRODUCTION Designing, upgrading or optimizing a laboratory for human assisted reproduction technology (commonly referred to simply as “ART” or “IVF” for in-vitro fertilization) is best tackled using a systems approach combined with process control. This can be summarized in the following four basic steps. ! Define all the inputs to the system. These include equipment, consumables and biological materials, as well as operational (technical) procedures and extrinsic factors such as uncontrolled environmental conditions and external (e.g. third-party) influences. ! Define every thing that happens during the entire process, paying special attention to interactive factors. ! Identify and define sentinel indicators for all stages of the entire process so that stage-specific outcomes can be monitored. ! Design strategies to eliminate or correct all suspected adverse influences that can affect equipment, component processes or procedures. Each aspect of the strategy must include not only the perceived remedy (effector) but also a scheme whereby its impact can be monitored. Clearly, therefore, one must understand how important pieces of equipment operate, and how their operation can be affected by their incorrect use or extrinsic environmental and materials factors. Design specifications and engineering factors can also be important inputs into the analyses, as well as manufacturing standards and QC/QA of components. EXTERNAL FACTORS AFFECTING OOCYTES AND EMBRYOS IN VITRO During all handling of oocytes and embryos they must be protected from extrinsic sources of physical and chemical stress. Such factors can be grouped into three major areas: (1) temperature changes; (2) culture medium osmolarity and pH; and (3) air quality, although they do interact in various ways.
    [Show full text]
  • Basic Concept Laboratory Tech M
    Basic concept in Laboratory Techniques M. Sc. 2nd Semester Subject: Basic Concepts in Laboratory Techniques 1 Code: PGS – 504 (0 + 1) Chemical Use Guideline 1) Never use a product that doesn't have a label to reference. 2) Don't mix chemicals without specific authorization from the formulator. 3) Always use personal protective equipments. 4) When pouring chemicals, pour concentrates into the water and not vice-versa. 5) Never pour chemicals into an empty, unlabeled container. 6) Don't store flammable chemicals near a source of heat. 7) Pesticides, fungicides, etc. always must be stored in a safe and elevated position. Basically, there are four types of chemicals • Toxic chemicals : These are chemicals that are poisonous to you, and can act upon the body very rapidly. Hydrogen sulfide and cyanide are examples of toxic agents. • Corrosives : This type of chemical is usually an irritant. Corrosives can damage your body by burning, scalding or inflaming body tissues. Examples are chlorine and HCl acid. • Flammables : Flammables are the chemicals that burn readily. They may explode or burn if sparks, flames or other ignition sources are present. Examples are gasoline, benzene and ethyl ether. • Reactive : Reactive chemicals are those that require stability and careful handling. Some of them can explode or react violently if the container is dropped or hit. Nitroglycerine is an example. Basic Tips of Safe Chemical Handling 1. Read the label 2. Dress the part 3. Follow directions 4. Know emergency procedures 5. Be careful! 6. Report any suspected problems 7. Keep your work area neat, clean and organized 8.
    [Show full text]
  • Hydrodynamic Shear Sensitivity of Suspension Cultured Plant Cells
    1i.o1 Hydrodynamic Shear Sensitivity of Suspension Cultured Plant Cells by WONG Vai Tak Victor Thesis submitted for the degree of Doctor of Philosophy in The University of Adelaide Department of Chemical Engineering Faculty of Engineering July 2001 ) AcxxowLEDGMENTS of several individuals and I would like to acknowledge the invaluable contributions like to thank my supervisors Dr David organisations towards this work. Firstly, I would their guidance and williams, Dr Christopher colby and Dr David saint for taking the time to edit this thesis and encouragement throughout the project, and for thank Prof Eric Dunlop for his advice and suggest useful changes. I would also like to help in the early periods of the project' setting up the Plant Cell Culture I am grateful to Mr Chris Mansell for his help with well as to Mrs Mary Barrow and Mrs laboratory and for his technical assistance, as I am particularly indebted to the Elaine Minerds for their wonderful secretarial support' Peter Kay, for providing their practical departmental workshop staff, especially Mr viscometer' I would also like to thank expertise in the construction of the novel Couette Department of Environmental Biology at Dr Joe v/iskich and Mz Lidia Mischis of the DO probes and their invaluable advice The University of Adelaide, for the loan of the regarding the oxygen uptake rate measurements' FinancialsupportforthisworkwasprovidedbyanAustralianResearchCouncil scholarship and for contributing grant. I am grateful for their award of a postgraduate towards the operating expenses incurred' IwouldliketothankmyfellowpostgraduatestudentswithintheDepartmentof and discussion' Being an overseas student Chemical Engineering for their camaraderie friends, both in and outside the university' in a foreign land, the emotional support of periods during the project' Although I cannot was essential for getting through difficult to thank Rev.
    [Show full text]
  • ROBERT VANDERPOOL SURENDER KAUSHIK Sania W. Tong Argao
    U.S Environmental Protection Agency Office of Research and Development National Exposure Research Laboratory Exposure Methods and Measurements Division Air Quality Branch STANDARD OPERATING PROCEDURE SOP Title: Collection and Extraction of Challenge Aerosol for Subsequent Coulter Analysis SOP ID: D-EMMD-AQB-030-SOP-01 Effective Date: November 13, 2015 SOP was Developed: ☐ In-house ☒ Extramural: Jacobs SOP Discipline*: General Chemistry Alternative Identification: SOP-J15-002.0 SOP Contact Signature Name: Robert Vanderpool Signature/Date: Management Signature Name: Surender Kaushik Title: AQB Branch Chief Signature/Date: QA Signature Name: Sania W. Tong Argao Title: EMMD QA Manager Signature/Date: *See discipline descriptions on the NERL Scientific and Technical SOP intranet site DISCLAIMER: These procedures are limited to use by, or under direction of, researchers and technicians who have demonstrated proficiency with the procedures. This document has not been through the Agency's peer review process nor the EMMD clearance/publication process. This is NOT an official EPA approved method. Challenge Aerosol for Coulter Analysis SOP-J15-002.0 Revision 0 November 2015 Page 1 of 21 Collection and Extraction of Challenge Aerosol for Subsequent Coulter Analysis SOP-J15-002.0 Revision of SOP-ZD-14-02(1) Contract EP-C-15-008 November 2015 Prepared for Process Modeling Research Branch National Exposure Research Laboratory U.S. Environmental Protection Agency Research Triangle Park, NC 27711 Prepared by: Date: 11/13/15 Carlton Witherspoon, Jacobs Senior Scientist Approved by: Date: 11/13/15 Laurie Brixey, Jacobs Department Manager Date: 11/13/15 Zora Drake-Richman, Jacobs QA Officer Challenge Aerosol for Coulter Analysis SOP-J15-002.0 Revision 0 November 2015 Page 2 of 21 Contents Figures .........................................................................................................................................................
    [Show full text]