Laboratory Catalog
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Bezanilla Lab Moss Methods Manual
Bezanilla Lab Moss Methods Manual April 2008 Updated July 2012 Contents 1. Tissue Propagation 3 2. Tissue Storage 8 3. Generating Protoplasts 9 4. Transformation 11 5. DNA Isolation 14 6. Protein Isolation 15 7. GUS Assays 17 8. RNA Interference 18 9. 2-Fragment Recombination for N- and -C terminal tagging 23 10. 3-Fragment Recombination for generating knock out constructs 24 11. 4-Fragment Recombination for generating knock in constructs 26 Appendix I – Moss Growth Media Recipes 31 Appendix II – Moss Transformation Reagents 35 Appendix III – Cloning Reactions 38 2 Tissue Propagation Moss tissue should be propagated weekly. For routine culturing of moss, the following unusual items are needed: Cellophanes Tissue homogenizer These items can be acquired from the following sources: Cellophanes: Gel drying cellophanes from Research Products International, catalog #1080 It is ideal to have the cellophanes cut in circles the appropriate size for standard disposable Petri dishes. It is possible to acquire a "hole" punch that will cut out circles of the correct size from the following arts and crafts site: http://www.1stopsquare.com/gigapunc.html Order the Paper Punch Craft, Marvy Uchida Giga - Circle 3". Tissue Homogenizer: Powergen 125 homogenizer – Fisher Scientific 14-261-02 Adaptor for disposable generators – Fisher Scientific 14-261-35 Disposable generators 110 mm in length – Fisher Scientific 14-261-26 These generators can be placed in glass culture tubes, covered with aluminum foil and autoclaved. After use they can be washed in water, air- dried and re-autoclaved. Typically these generators can be re- autoclaved at least 20 times. -
2015 CATALOG the Homogenizer Company™
The Homogenizer Company™ 2015 CATALOG Innovative Solutions Omni International’s homogenizers have been a laboratory staple for over 50 years. Omni sets the indus- try standard with a commitment to outstanding design, performance and a uniquely diversified solution- based product line. We offer a complete portfolio of homogenizers designed for pharmaceutical, life science, biotechnology, agricultural, microbiology and chemical research and development. Our business is strictly homogenizer-focused and we offer the broadest range of solutions to process any sample, including high shear, ultrasonic, mortar and pestle, and bead mill homogenizers. In response to increased activity in global infectious disease research, Omni was first in designing and developing a patented plastic disposable generator probe, the Omni Tip™, which completely eliminates cross-contamination between samples. Our multi-sample and automated homogenizers set the standard by removing the repetitive labor-intensive work of processing samples one at a time. Customer Service Our staff of engineers and technicians are dedicated to finding solutions that improve sample processing, reduce noise, and improve sample processing efficiency, while our support team works to assure that we routinely exceed our customers’ expectations. We are confident that you will find our products and service superior to others. Omni International offers no-risk product evaluations for your convenience. Quality and Convenience Omni products offer the most cost-effective choice for quality, service, and convenience to effectively and ef- ficiently process all types of sample preparation applications, usually in 30 seconds or less. We offer the broadest choice of accessories to process the widest range of samples with user-friendly designs that are easy to clean and maintain. -
Supplement of Atmos
Supplement of Atmos. Chem. Phys., 15, 2489–2518, 2015 http://www.atmos-chem-phys.net/15/2489/2015/ doi:10.5194/acp-15-2489-2015-supplement © Author(s) 2015. CC Attribution 3.0 License. Supplement of A comprehensive laboratory study on the immersion freezing behavior of illite NX particles: a comparison of 17 ice nucleation measurement techniques N. Hiranuma et al. Correspondence to: N. Hiranuma ([email protected]) 1 S1. Supplementary Methods 2 3 This supplementary information provides additional details for the measurement 4 techniques of immersion freezing of illite NX particles with S1.1. suspension techniques and 5 S1.2. dry-dispersed particle measurement techniques (both in alphabetical order as in Table 6 1). The discussions of measurement uncertainties of temperature and ns for each measurement 7 technique are also provided. We note that the uncertainty in frozen fraction (α) used in 8 calculating ns may not be adequate, since the sensitivity of Δα (an increase or a decrease in 9 frozen fraction) is much higher at high temperatures which unexceptionally coincide with a 10 low fraction of frozen illite NX. 11 12 S1.1. Suspension techniques 13 14 Bielefeld Ice Nucleation ARraY (BINARY) 15 16 The BINARY setup is an optical freezing apparatus that makes use of the change in 17 droplet brightness during freezing for the automated and simultaneous detection of ice 18 nucleation in 36 microliter-sized droplets. The droplets are positioned on a hydrophobic glass 19 slide that rests on top of a Peltier cooling stage (Linkam LTS 120). The 36 droplets are 20 separated from each other by a polydimethylsiloxane (PDMS) spacer in order to prevent a 21 Wegener-Bergeron-Findeisen process. -
Effective Droplet Size Reduction and Excellent Stability of Limonene
colloids and interfaces Article Effective Droplet Size Reduction and Excellent Stability of Limonene Nanoemulsion Formed by High-Pressure Homogenizer Marcel Jonathan Hidajat 1 , Wantaek Jo 2, Hyeonhyo Kim 2 and Jongho Noh 2,* 1 Department of Research and Development, Hiflux Co. Ltd., 361-33, Gapcheon-ro, Daejeon 34037, Korea; [email protected] 2 Department of Research and Development, Ilshin Autoclave Co. Ltd., 255, Techno 2-ro, Daejeon 34026, Korea; jowt@suflux.com (W.J.); hh@suflux.com (H.K.) * Correspondence: njh@suflux.com; Tel.: +82-42-9316100 Received: 18 December 2019; Accepted: 8 January 2020; Published: 10 January 2020 Abstract: Limonene as an interesting bioactive material that has great benefits due to its antimicrobial and anti-carcinogen properties. However, it has several limitations such as its oxidative and oily nature. In order to overcome these limitations, a high-pressure homogenizer (HPH) was utilized to produce limonene nanoemulsion, which enhances its dispersibility while preventing oxidation with great stability. Limonene was pre-mixed with soybean oil as carrier oil prior to emulsification. The effect of soybean oil to limonene ratio, number of pass, homogenization pressure, emulsifier concentration and homogenization method were observed. A stability test was also conducted for 28 days at room temperature. The result revealed that soybean oil and limonene demonstrated a certain ratio to produce the most stable nanoemulsion. Meanwhile, emulsion size could be reduced from 327.8 nm to 55.5 nm in five passes at 1000 bar. Increasing the emulsifier concentration could reduce the droplet size to 40 nm. A comparison with other emulsification method showed that HPH was the best emulsification technique due to its intense emulsification power resulted from shear, cavitation, and droplet impacts. -
Biological and Chemical Safety Manual
Biological and Chemical Safety Manual Fall, 2012 1 EMERGENCY INFORMATION (Fill out laboratory specific information and post outside of laboratory – copy to EHS dept.) Principal Investigator After -Hours Contact Information Lab Location Location Of Chemical Spill Kit Location Of Biological Spill Kit Location Of Fire Extinguisher Location Of Fire Alarm Location of Eye Wash Station Location of Safety Shower USD Environmental Health and Safety Chemical & Radiation Safety Kevin O’Kelley, Director of Environmental Health & Safety [email protected] , 605-677-6265 Institutional Review Board (IRB) Sandra Ellenbolt, Director of Human Subjects Protection [email protected] , 605-677-6067 Institutional Animal Care and Use Committee (IACUC) Peter Autenried, Director of Animal Resource Center [email protected] , 605-677-5174 Institutional Biosafety Committee (IBC) Victor Huber, Ph.D. [email protected] , 605-677-5163 Facilities Management Call Desk: 605-677-5341 (After hours, call USD Public Safety) USD Public Safety Office: 605-677-5342 Fire/Police/Emergency Medical Services Dispatch: 9-911 2 Preface A safe working and learning environment is an expectation of USD and is provided to all employees, students, and guests at the highest level reasonably possible. The University of South Dakota Biological and Chemical Safety Policy is designed as a reference for individual laboratories to provide a safe and productive work environment while complying with applicable federal and state rules and best practices. Each laboratory group should supplement this policy when necessary to insure health and safety of workers is not compromised. The policy is divided into Chemical Safety and Biological Safety sections. Radiation Safety is addressed in the University of South Dakota’s Radiation Safety Policy for Authorized Workers. -
Food Homogenization
The following is a section of the Elemental Analysis Manual for Food and Related Products. For additional information and to view other sections of the manual, visit the Elemental Analysis Manual for Food and Related Products web page at http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006954.htm. Elemental Analysis Manual for Food and Related Products 2.2 Food Homogenization September, 2014 Authors: William R. Mindak Richard M. Jacobs Stephen G. Capar William C. Cunningham Table of Contents 2.2.1 LABORATORY HOMOGENIZATION EQUIPMENT 2.2.2 HOMOGENIZATION PROCEDURES 2.2.2.1 GENERAL PROCEDURES 2.2.2.2 CANDY PROCEDURES 2.2.2.3 PILLS, CAPSULES, SUPPLEMENTS, ETC. GLOSSARY This section provides information to assist the analyst on physically preparing the analytical sample prior to taking an analytical portion for analysis. Obtaining representative analytical portions is critical for accurate and reproducible results. EAM methods assume the analyst has made the analytical sample homogeneous with respect to the size of the analytical portion. Homogenized foods will vary greatly in texture and viscosity and may separate into liquid and solid components. Analytical samples that have been stored after being homogenized usually require re-homogenization before acquiring an analytical portion. The analyst must carefully observe the physical characteristics of the homogenate and choose an appropriate means of obtaining a representative analytical portion (e.g., pouring directly from container possibly during active mixing, using a 1, 5 or 10 mL pipette or using a spatula). Elemental Analysis Manual (Section 2.2 Food Homogenization) Note: If non-homogeneity is known or suspected then replicate analytical portions should be analyzed and the results assessed. -
(Title of the Thesis)*
In-Situ Continuous Monitoring of Catastrophic Phase Inversion and Viscosity of Pickering Emulsions by Upinder Bains A thesis presented to the University of Waterloo in fulfillment of the thesis requirement for the degree of Master of Applied Science in Chemical Engineering Waterloo, Ontario, Canada, 2018 ©Upinder Bains 2018 AUTHOR'S DECLARATION I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ii Abstract Emulsions are used in the various field such as petroleum, pharmaceuticals, foods, cosmetics, paints, etc. Emulsions stabilized with solid nanoparticles are called Pickering emulsions. More recently, a growing awareness of using environment friendly products has led to more and more researchers to develop and modify natural materials. Starch nanoparticle might be a suitable candidate because they are environmentally friendly, safe and non-toxic. Also, from a practical point of view, starch nanoparticles are low cost, and rheological properties of their emulsions can easily be altered with the help of additives. Because of the unstable nature of these emulsions, continuous agitation is needed to keep the emulsion from separating. This makes the viscosity measurements a challenge, however this task was made possible at low shear rates under some controlled parameters. In this study, an in-situ viscosity measurement method is used to investigate the viscous behaviour of O/W and W/O emulsions at a different volume fraction of the dispersed phase. For this purpose, rotational viscometer installed in a tank was used along with a high shear mixer. -