Laboratory Biosafety Manual Third Edition
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Tremprime QD Low-Odor
Version: 1.1 Revision Date: 10/12/2018 SAFETY DATA SHEET 1. Identification Material name: TREMPRIME Q.D. LOW ODOR Material: 022045 805 Recommended use and restriction on use Recommended use : Coatings Restrictions on use: Not known. Manufacturer/Importer/Supplier/Distributor Information Tremco Canadian Sealants 220 Wicksteed Ave Toronto ON M4H 1G7 CA Contact person : EH&S Department Telephone : 1-800-263-6046 Emergency telephone number : 1-800-424-9300 (US); 1-613-996-6666 (Canada) 2. Hazard(s) identification Hazard Classification Physical Hazards Flammable liquids Category 3 Health Hazards Acute toxicity (Inhalation - vapor) Category 4 Serious Eye Damage/Eye Irritation Category 2A Germ Cell Mutagenicity Category 1B Carcinogenicity Category 1B Unknown toxicity - Health Acute toxicity, oral 40.8 % Acute toxicity, dermal 43 % Acute toxicity, inhalation, vapor 97 % Acute toxicity, inhalation, dust 100 % or mist Label Elements Hazard Symbol : 1/14 800000060000 Version: 1.1 Revision Date: 10/12/2018 Signal Word: Danger Hazard Statement: Flammable liquid and vapor. Harmful if inhaled. Causes serious eye irritation. May cause genetic defects. May cause cancer. Precautionary Statements Prevention: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. Keep container tightly closed. Ground and bond container and receiving equipment. Use explosion-proof [electrical/ventilating/lighting/EF e:uipment. 5se non1sparking tools. Take action to prevent static discharges. Wear protective gloves/protective clothing/eye protection/face protection. Avoid breathing dust/fume/gas/mist/vapors/spray. 5se only outdoors or in a well1ventilated area. Wash thoroughly after handling. Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. -
Biohazardous Waste Treatment
Biological Safety: Principles & Applications for Lab Personnel Presented by: Biological Safety Office http://biosafety.utk.edu Introduction OVERVIEW OF BIOSAFETY PROGRAM Introduction to Laboratory Safety When entering the laboratory environment you are likely to contact hazards that you would not encounter on a daily basis. These can include: • Physical hazards • Chemical hazards • Radiological hazards • Biological hazards Biohazards Any biological agent or condition that poses a threat to human, animal, or plant health, or to the environment. Examples include, but not limited to: • Agents causing disease in humans, animals or plants (bacteria, viruses, etc.) • Toxins of biological origin • Materials potentially containing infectious agents or biohazards .Blood, tissues, body fluids etc. .Waste, carcasses etc. • Recombinant DNA (depends) • Nanoparticles w/biological effector conjugates (depends) Types of Biohazards: What they are.... How they’ll look.... Regulations & Standards The Institutional Biosafety Committee Institutional Biosafety Committees (IBC) are required by the National Institutes of Health Office of Biological Activities (NIH; OBA) for institutions that receive NIH funding and conduct research using recombinant or synthetic nucleic acids. The UT IBC is composed of 14 UT-affiliated members and 2 non-affiliated members, who collectively offer a broad range of experience in research, safety and public health. Functions of the IBC Projects requiring IBC review • Performing both initial and annual • Those involving recombinant -
Use of Laboratory Equipement
USE OF LABORATORY EQUIPEMENT C. Laboratory Thermometers Most thermometers are based upon the principle that liquids expand when heated. Most common thermometers use mercury or colored alcohol as the liquid. These thermometers are constructed as that a uniform-diameter capillary tube surmounts a liquid reservoir. To calibrate a thermometer, one defines two reference points, normally the freezing point of water (0°C, 32°F) and the boiling point of water (100°C, 212°F) at 1 tam of pressure (1 tam = 760 mm Hg). Once these points are marked on the capillary, its length is then subdivided into uniform divisions called degrees. There are 100° between these two points on the Celsius (°C, or centigrade) scale and 180° between those two points on the Fahrenheit (°F) scale. °F = 1.8 °C + 32 The Thermometer and Its Calibration This section describes the proper technique for checking the accuracy of your thermometer. These measurements will show how measured temperatures (read from thermometer) compare with true temperatures (the boiling and freezing points of water). The freezing point of water is 0°C; the boiling point depends upon atmospheric pressure but at sea level it is 100°C. Option 1: Place approximately 50 mL of ice in a 250-mL beaker and cover the ice with distilled water. Allow about 15 min for the mixture to come to equilibrium and then measure and record the temperature of the mixture. Theoretically, this temperature is 0°C. Option 2: Set up a 250-mL beaker on a wire gauze and iron ring. Fill the beaker about half full with distilled water. -
Equine Biosecurity and Biocontainment Practices on U.S
Veterinary Services Centers for Epidemiology and Animal Health November 2006 _________________________________________________________________________________________________________________________ Equine Biosecurity and Biocontainment Practices on U.S. Equine Operations With the globalization of animal industries, biosecurity General Practices and biocontainment practices play a vital role in the health of domestic animals. Biosecurity practices include General biosecurity measures taken on equine measures that reduce the risk of disease introduction on operations include isolation protocols, infection-control an operation. Biocontainment practices include precautions for visitors, and use of separate or measures that reduce the spread of disease on an disinfected equipment. Other general management operation and from one operation to another. practices address contact with other animals, potential Vaccination, another important aid in infection control, contamination of feed and water, insect control, and will be addressed in a separate information sheet. manure management. Equine diseases are spread in a variety of ways, including direct contact between equids and contact with Separation for isolation or infection control feces, insect or animal vectors, aerosol particles, and contaminated needles. Often overlooked but equally Overall, 65.1 percent of operations separated important modes of disease transmission include contact animals for isolation or infection control. For this study, with contaminated equipment, tack, transport -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
Bezanilla Lab Moss Methods Manual
Bezanilla Lab Moss Methods Manual April 2008 Updated July 2012 Contents 1. Tissue Propagation 3 2. Tissue Storage 8 3. Generating Protoplasts 9 4. Transformation 11 5. DNA Isolation 14 6. Protein Isolation 15 7. GUS Assays 17 8. RNA Interference 18 9. 2-Fragment Recombination for N- and -C terminal tagging 23 10. 3-Fragment Recombination for generating knock out constructs 24 11. 4-Fragment Recombination for generating knock in constructs 26 Appendix I – Moss Growth Media Recipes 31 Appendix II – Moss Transformation Reagents 35 Appendix III – Cloning Reactions 38 2 Tissue Propagation Moss tissue should be propagated weekly. For routine culturing of moss, the following unusual items are needed: Cellophanes Tissue homogenizer These items can be acquired from the following sources: Cellophanes: Gel drying cellophanes from Research Products International, catalog #1080 It is ideal to have the cellophanes cut in circles the appropriate size for standard disposable Petri dishes. It is possible to acquire a "hole" punch that will cut out circles of the correct size from the following arts and crafts site: http://www.1stopsquare.com/gigapunc.html Order the Paper Punch Craft, Marvy Uchida Giga - Circle 3". Tissue Homogenizer: Powergen 125 homogenizer – Fisher Scientific 14-261-02 Adaptor for disposable generators – Fisher Scientific 14-261-35 Disposable generators 110 mm in length – Fisher Scientific 14-261-26 These generators can be placed in glass culture tubes, covered with aluminum foil and autoclaved. After use they can be washed in water, air- dried and re-autoclaved. Typically these generators can be re- autoclaved at least 20 times. -
Field Trials for GM Food Get Green Light in India
NATURE|Vol 448|23 August 2007 NEWS IN BRIEF Field trials for GM food Asthmatics win payment in diesel-fumes lawsuit get green light in India Asthma patients in Tokyo last week welcomed a cash settlement India’s first genetically modified (GM) food from car manufacturers and crop is a step closer to reaching the dining the Japanese government. The table. The government has approved field one-time payment resolved a trials for a strain of brinjal (aubergine) decade-long legal battle in which carrying a Bt (Bacillus thuringiensis) gene. the asthmatics blamed diesel car Jalna-based Mahyco, the Indian venture fumes for their illness. KIM KYUNG-HOON/REUTERS of US seed giant Monsanto, claims its insect- The automakers, including resistant variety gives better yields with Toyota, Honda and Nissan, will less pesticide use. To avoid possible cross- provide ¥1.2 billion (US$10.5 contamination with farmers’ crops, the trials million) to the plaintiffs, and a will be carried out in government farms. further ¥3.3 billion to support But critics already campaigning against Bt a five-year health plan for the cotton — currently the only GM crop grown patients. The central government in India — say the brinjal trials are illegal. and Tokyo metropolitan Full biosafety data on the brinjal tests government will each contribute ¥6 billion to the medical programme. have not yet been generated, says Kavitha Kuruganti of the Centre for Sustainable scientists — to pay for relocation, housing starting dose “should be considered for Agriculture, based in Hyderabad: “The trials and research. “This is about the rescue of patients with certain genetic variations”. -
Lightweight Django USING REST, WEBSOCKETS & BACKBONE
Lightweight Django USING REST, WEBSOCKETS & BACKBONE Julia Elman & Mark Lavin Lightweight Django LightweightDjango How can you take advantage of the Django framework to integrate complex “A great resource for client-side interactions and real-time features into your web applications? going beyond traditional Through a series of rapid application development projects, this hands-on book shows experienced Django developers how to include REST APIs, apps and learning how WebSockets, and client-side MVC frameworks such as Backbone.js into Django can power the new or existing projects. backend of single-page Learn how to make the most of Django’s decoupled design by choosing web applications.” the components you need to build the lightweight applications you want. —Aymeric Augustin Once you finish this book, you’ll know how to build single-page applications Django core developer, CTO, oscaro.com that respond to interactions in real time. If you’re familiar with Python and JavaScript, you’re good to go. “Such a good idea—I think this will lower the barrier ■ Learn a lightweight approach for starting a new Django project of entry for developers ■ Break reusable applications into smaller services that even more… the more communicate with one another I read, the more excited ■ Create a static, rapid prototyping site as a scaffold for websites and applications I am!” —Barbara Shaurette ■ Build a REST API with django-rest-framework Python Developer, Cox Media Group ■ Learn how to use Django with the Backbone.js MVC framework ■ Create a single-page web application on top of your REST API Lightweight ■ Integrate real-time features with WebSockets and the Tornado networking library ■ Use the book’s code-driven examples in your own projects Julia Elman, a frontend developer and tech education advocate, started learning Django in 2008 while working at World Online. -
2015 CATALOG the Homogenizer Company™
The Homogenizer Company™ 2015 CATALOG Innovative Solutions Omni International’s homogenizers have been a laboratory staple for over 50 years. Omni sets the indus- try standard with a commitment to outstanding design, performance and a uniquely diversified solution- based product line. We offer a complete portfolio of homogenizers designed for pharmaceutical, life science, biotechnology, agricultural, microbiology and chemical research and development. Our business is strictly homogenizer-focused and we offer the broadest range of solutions to process any sample, including high shear, ultrasonic, mortar and pestle, and bead mill homogenizers. In response to increased activity in global infectious disease research, Omni was first in designing and developing a patented plastic disposable generator probe, the Omni Tip™, which completely eliminates cross-contamination between samples. Our multi-sample and automated homogenizers set the standard by removing the repetitive labor-intensive work of processing samples one at a time. Customer Service Our staff of engineers and technicians are dedicated to finding solutions that improve sample processing, reduce noise, and improve sample processing efficiency, while our support team works to assure that we routinely exceed our customers’ expectations. We are confident that you will find our products and service superior to others. Omni International offers no-risk product evaluations for your convenience. Quality and Convenience Omni products offer the most cost-effective choice for quality, service, and convenience to effectively and ef- ficiently process all types of sample preparation applications, usually in 30 seconds or less. We offer the broadest choice of accessories to process the widest range of samples with user-friendly designs that are easy to clean and maintain. -
Anastasi 2032
Shashwat Goel & Ankita Phulia Anastasi 2032 Table of Contents Section Page Number 0 Introduction 2 1 Basic Requirements 4 2 Structural Design 15 3 Operations 31 4 Human Factors 54 5 Business 65 6 Bibliography 80 Fletchel Constructors 1 Shashwat Goel & Ankita Phulia Anastasi 2032 0 Introduction What is an underwater base doing in a space settlement design competition? Today, large-scale space habitation, and the opportunity to take advantage of the vast resources and possibilities of outer space, remains more in the realm of speculation than reality. We have experienced fifteen years of continuous space habitation and construction, with another seven years scheduled. Yet we have still not been able to take major steps towards commercial and industrial space development, which is usually the most-cited reason for establishing orbital colonies. This is mainly due to the prohibitively high cost, even today. In this situation, we cannot easily afford the luxury of testing how such systems could eventually work in space. This leaves us looking for analogous situations. While some scientists have sought this in the mountains of Hawaii, this does not tell the full story. We are unable to properly fathom or test how a large-scale industrial and tourism operation, as it is expected will eventually exist on-orbit, on Earth. This led us to the idea of building an oceanic base. The ocean is, in many ways, similar to free space. Large swathes of it remain unexplored. There are unrealised commercial opportunities. There are hostile yet exciting environments. Creating basic life support and pressure-containing structures are challenging. -
Concept Study of a Cislunar Outpost Architecture and Associated Elements That Enable a Path to Mars
Concept Study of a Cislunar Outpost Architecture and Associated Elements that Enable a Path to Mars Presented by: Timothy Cichan Lockheed Martin Space [email protected] Mike Drever Lockheed Martin Space [email protected] Franco Fenoglio Thales Alenia Space Italy [email protected] Willian D. Pratt Lockheed Martin Space [email protected] Josh Hopkins Lockheed Martin Space [email protected] September 2016 © 2014 Lockheed Martin Corporation Abstract During the course of human space exploration, astronauts have travelled all the way to the Moon on short flights and have logged missions of a year or more of continuous time on board Mir and the International Space Station (ISS), close to Earth. However, if the long term goal of space exploration is to land humans on the surface of Mars, NASA needs precursor missions that combine operating for very long durations and great distances. This will allow astronauts to learn how to work in deep space for months at a time and address many of the risks associated with a Mars mission lasting over 1,000 days in deep space, such as the inability to abort home or resupply in an emergency. A facility placed in an orbit in the vicinity of the Moon, called a Deep Space Transit Habitat (DSTH), is an ideal place to gain experience operating in deep space. This next generation of in-space habitation will be evolvable, flexible, and modular. It will allow astronauts to demonstrate they can operate for months at a time beyond Low Earth Orbit (LEO). The DSTH can also be an international collaboration, with partnering nations contributing elements and major subsystems, based on their expertise. -
Biological Agents in the Laboratory
PUBLIC INTEREST REPORT FALL 2011 BACKGROUND BRIEF HISTORY OF BIOSAFETY Within three weeks of the destruction of the World Trade Center towers on September Innovation and development of biosafety 11, 2001, the United States experienced a second assault in the form of anthrax spores in the United States is reflected accurately delivered through the U.S. mail. The event in the history and pre-history of the initiated widespread changes in the scientific American Biological Safety Association enterprise of the United States, in its (ABSA). The first unofficial meeting was federally-based funding priorities and in the held on April 18, 1955 at Camp Detrick regulatory and oversight mechanisms that (now Fort Detrick) and involved strive to keep laboratories and communities members of the military representing safe. Camp Detrick, Pine Bluff Arsenal, Arkansas (PBA), and Dugway Proving “The events of September 11, 2001, and the Grounds, Utah (DPG). In those days, the anthrax attacks in October of that year re- offensive BW program of the United shaped and changed, forever, the way we States was in full swing: the opening manage and conduct work in biological and keynote address was “The Role of Safety clinical laboratories.”1 in the Biological Warfare Effort.” Beginning in 1957, the yearly meetings Biosafety and biosecurity have dominated began to include non-classified sessions to the policy discourse and the two have been broaden the reach of the Association; inexorably intertwined. Biosafety and representatives of the USDA were regular biosecurity are defined by the World Health 2 attendees through this “transition Organization (WHO): Biosafety comprises 4 “the containment principles, technologies period.” There were striking changes in and practices that are implemented to the meetings in 1964-1965: the NIH and prevent unintentional exposure to pathogens CDC joined for the first time, along with and toxins or their accidental release”; a number of other relevant federal biosecurity is defined as “the protection, agencies.