SHORT NOTE HERPETOZOA 26 (3/4) Wien, 30. Jänner 2014 SHORT NOTE 195

New Brazilian records of unequivocal and associated to precise coor - Leptodactylus chaquensis CEi , 1950, dinates. Photographs of live specimens from the three new localities (Figs. 1a, 1b, at the species’ southern range limit 1c) were taken in the field and used to con - firm species identity, together with voucher The Neotropical genus Leptodactylus specimens (collected under the permits FiTZiNgER , 1826, currently includes 89 rec - iBAMA / SiSBiO #25916-1 and iAP ognized species and is widely distributed #278.10). The specimens were euthanized from southern North America to central Ar- with 5 % lidocaine gel, preserved in 10 % gentina, throughout most of South America formalin, and stored in alcohol 70 %. Four- (FROST 2013). The genus is divided in five teen vouchers were subsequently deposited species groups: L. fuscus , L. latrans , L. mar - at the Coleção Zoológica da Universidade moratus , L. melanonotus , and L. pentadac - Federal de goiás (ZUFg; goiânia, state of tylus (HEyER 1969; FROST 2013). Belong - goiás, Brazil), under the assessment numbers ing to the L. latrans group, Cei’s White- ZUFg 7146, 7195-7200, 7202-7206, 7492, lipped Frog Leptodactylus chaquensis CEi , 7493. 1950, is a mid-sized species (males and fe- The authors recorded specimens of males reaching 71 mm snout-vent length) Leptodactylus chaquensis in three localities that inhabits both permanent and temporary in the northwestern portion of the state of aquatic habitats ( UETANABARO et al. 2008). Paraná, near the borders with the Brazilian The geographic distribution of Lepto - state of Mato grosso do Sul and with dactylus chaquensis encompasses northern Paraguay. in the municipality of diamante Argentina (Catamarca, Chaco, Córdoba, do Norte, this species was recorded during Corrientes, Entre Ríos, Formosa, Jujuy, La fieldwork carried out between April 2011 Rioja, Misiones, Mendoza, Salta, San Juan, and January 2013 at Estação Ecológica do San Luis, Santiago del Estero, Santa Fe, Caiuá (22°37’17”S, 52°50’17”W, 268 m Tucumán), the Chaco and western oriental a.s.l.) and surrounding areas. At this loca - region of Paraguay, northern Uruguay tion, specimens of L. chaquensis were found (Artigas and Salto), lowland Bolivia, and in both permanent and temporary ponds Brazil ( HEyER et al. 2004; ACOSTA et al. within pasture area, sugar cane and cassava 2005; VAiRA et al. 2012; FROST 2013). in plantations, as well as in permanent im - the latter country, this species was reported poundments in two small tributaries of the to occur in the states of Acre ( SOUZA 2009), Paranapanema River (Água Mole and Maria Mato grosso ( HEyER & MUñOZ 1999; Koss streams) and in the margins of the STRüSSMANN 2000; CAMPOS FiLHO 2009; Paranapanema and Corvo Rivers, both PANSONATO et al. 2011; SANTOS et al. 2011), belonging to the upper Paraná River basin. Mato grosso do Sul ( STRüSSMANN et al. More than 50 individuals (12 of which were 2000; ÁViLA & FER REiRA 2004; gORdO & collected: 10 adult males and two adult CAMPOS 2004; PRA dO & HAddAd 2003, females) were recorded, following sampling 2005; PRAdO et al. 2000, 2005; UETANA- efforts of around 110 hours/observer. BARO et al. 2007; PiATTi et al. 2010; SOUZA in the municipality of icaraíma, ap - et al. 2010), Minas gerais ( SiLVEiRA 2006; proximately 40 specimens were recorded on giARETTA et al. 2008), Rondônia ( CAL- 07 February 2013, in the margins of a per - dERON et al. 2009), Rio grande do Sul manent pond surrounded by pasture (23°23’ (gARCiA & ViNCiPROVA 1998; SANTOS & 06”S, 53°45’55”W, 255 m a.s.l.). in the CECHiN 2008; TRiNdAdE et al. 2010), and municipality of guaíra, two specimens were São Paulo ( VASCONCELOS & ROSSA -FERES collected on 16 February 2013, also in a 2005; SANTOS et al. 2007, 2009; BRASSALOTi perma nent pond surrounded by pasture (24° et al. 2010). Herein after, the authors report 14’22”S, 54°14’30”W, 327 m a.s.l.). on the presence of L. chaquensis in the state Records of Leptodactylus chaquensis of Paraná and provide a distribution map in - in northwestern Paraná represent the first cluding literature records for this species in state record and fill a gap of ca. 850 km Brazil. Localities listed in Table 1 are those between the municipalities of Teodoro for which identification of L. chaquensis is Sampaio, in the state São Paulo ( SANTOS et 196 SHORT NOTE HERPETOZOA 26 (3/4) Wien, 30. Jänner 2014 SHORT NOTE

Fig. 1: Adult specimens of Leptodactylus chaquensis CEi , 1950, recorded in the municipalities of: a - diamante do Norte, b - icaraíma, and c - guaíra, state of Paraná, southern Brazil. SHORT NOTE HERPETOZOA 26 (3/4) Wien, 30. Jänner 2014 SHORT NOTE 197

Table 1: Selected localities in Brazil, with records of Leptodactylus chaquensis CEi , 1950.

State (capitalized) Ecoregion geographic coordinates Source and locality (numbered) ACRE 1 Parque Nacional Amazon rainforest 08º15’S, 73º15’W SOUZA (2009) da Serra do divisor RONdÔNiA 2 Porto Velho Amazon rainforest 08º43’S, 63º53’W CALdERON et al. (2009) MATO gROSSO 3 Cáceres Pantanal 16º27’S, 58º09’W PANSONATO et al. (2011) 4 Chapada dos guimarães Cerrado 14º57’S, 55º41’W STRüSSMANN (2000) 5 Nobres Cerrado 14º41’S, 56º13’W SANTOS et al. (2011) 6 Poconé Pantanal 16º15’S, 56º22’W VALéRiO -B RUN et al. (2010) 6 Poconé Pantanal 16º 34’S, 57º01’W dORAdO -R OdRigUES et al. (2012) MATO gROSSO dO SUL 7 Corumbá Pantanal 19º34’S, 57º00’W PRAdO et al. (2000) 7 Corumbá Pantanal 19º34’S, 57º00’W PRAdO & H AddAd (2003) 7 Corumbá Pantanal 19º34’S, 57º00’W PRAdO & H AddAd (2005) 7 Corumbá Pantanal 19º34’S, 57º00’W PRAdO et al. (2005) 7 Corumbá Pantanal 18º58’S, 57º39’W ÁViLA & F ERREiRA (2004) 8 Parque Nacional Pantanal 21º08’ to 20º38’S UETANABARO et al. (2007) da Serra da Bodoquena 56º48’ to 56º44’W 9 Porto Murtinho Pantanal 21º40’ to 21°56’S SOUZA et al. (2010) 57°54’ to 57°34’W 10 Miranda Pantanal 20º05’S, 56º36’W PiATTi et al. (2010) MiNAS gERAiS 11 João Pinheiro Cerrado 17º44’S, 46º10’W SiLVEiRA (2006) 12 Araguari Cerrado 18º33’S, 48º03’W giARETTA et al. (2008) 13 Uberlândia Cerrado 18º55’S, 48º17’W giARETTA et al. (2008) 14 Perdizes Cerrado 19º12’S, 47º10’W giARETTA et al. (2008) SÃO PAULO 15 Nova itapirema Atlantic rainforest 21º04’S, 49º32’W VASCONCELOS & R OSSA -F ERES (2005) and Cerrado 16 Santa Fé do Sul Atlantic rainforest 20º11’S, 50º53’W SANTOS et al. (2007) 17 Teodoro Sampaio Atlantic rainforest 22º27’ to 22º40’S and Cerrado 52º10’ to 52º22’W SANTOS et al. (2009) 18 gália and Alvilândia Atlantic rainforest 22º24’S, 49º42’W BRASSALOTi et al. (2010) RiO gRANdE dO SUL 19 Bossoroca Pampa 28º43’S, 54º54’W gARCiA & V iNCiPROVA (1998) 20 São Vicente do Sul Pampa 29º41’S, 54º40’W gARCiA & V iNCiPROVA (1998) 21 Uruguaiana Pampa 29º45’S, 57º05’W gARCiA & V iNCiPROVA (1998) 22 São Sepé Pampa 30º15’S, 53º35’W 30º15’S, 53º34’W SANTOS & C ECHiN (2008) 23 Caçapava do Sul Pampa 30º31’S, 53º 29’W TRiNdAdE et al. (2010) PARANÁ 24 diamante do Norte Atlantic rainforest 22º37’S, 52º50’W New record; this study 25 icaraíma Atlantic rainforest 23º23’S, 53º45’W New record; this study 26 guaíra Atlantic rainforest 24º14’S, 54º14’W New record; this study al. 2009), and São Sepé, in the state of Rio decades ago, was estimated to not surpass grande do Sul ( SANTOS & C ECHiN 2008) 1 % ( CAMPOS 1999). The massive and per - (Fig. 2, Table 1). Originally covered by manent loss of native vegetation might Seasonally Semi-deciduous Forest ( MAACK have resulted in changes in community 1981), the northwestern region of Paraná composition, and in a successful coloniza - since the early 60’s has been subjected to tion of the area by more generalist species, intense logging, agriculture and livestock well adapted to open and disturbed areas. activities. Total area of remnants of the individuals of species belonging to the L. original vegetation in the region, nearly two latrans group (which presently includes L. 198 SHORT NOTE HERPETOZOA 26 (3/4) Wien, 30. Jänner 2014 SHORT NOTE

Fig. 2: distribution map of Leptodactylus chaquensis CEi , 1950. Black dots - records from literature (see Table 1 for locality numbers and literature sources); shaded area - geographic distribution according to iUCN ( HEyER et al. 2004); black stars - new records in the State of Paraná, Southern Brazil.

bolivianus BOULENgER , 1898 , L. chaquensis final English revision. Tainá dorado-Rodrigues helped CEi , 1950 , L. latrans (S TEFFEN , 1815) , and with the map. The Estação Ecológica do Caiuá, CORiPA and Nupélia provided logistical support during field - L. macrosternum MiRANdA -R iBEiRO , 1926 ; work. FHO, Pgg, VgB, iPA thank Capes and CNPq HEyER & d E SÁ 2011; FROST 2013) typical - for Master and Phd scholarships. CS is a CNPq ly live in open habitats, including highly grantee of research productivity fellowship, level 2 disturbed areas, and seldom occupy pristine (process # 309541/2012-3). habitats, even when occurring in forested REFERENCES: ACOSTA , R. & M ESONES , R. V. areas (as already reported, e.g., for L. & N úñEZ , A . (2005): Fauna de anuros en la ciudad de Salta, Argentina. - Revista de Biologia Tropical, San latrans – as L. ocellatus – in southeastern José; 53 (3-4): 569-575. ALMEidA -g OMES , M. (2010): Brazil, by ALMEidA -gOMES et al. 2010, and Anurofauna of an Atlantic rainforest fragment and its in southern Brazil, by LUCAS & MAROCCO surroundings in northern Rio de Janeiro State, Brazil.- 2011; and for L. macrosternum in Brazilian Brazilian Journal of Biology, São Carlos; 70 (3): 871- 877. ÁViLA , R. W. & F ERREiRA , V. L. (2004): Riqueza Amazonia, by PiNHEiRO et al. 2012). e densidade de vocalizações de anuros (Amphibia) em ACKNOWLEdgEMENTS: The authors are uma área urbana de Corumbá, Mato grosso do Sul, grateful to Valdir, José Cândido, Valdenir, Celso, Aline, Brasil.- Revista Brasileira de Zoologia, Curitiba; 21 Erick, Fagner, Fábio, Letícia, Rodrigo, and Rogério for (4): 887-892. BRASSALOTi , R. A. & R OSSA -F ERES , d. field assistance, and to Jeffrey Himmelstein, for the C. & B ERTOLUCi , J. (2010): Anuran fauna of the semi- SHORT NOTE HERPETOZOA 26 (3/4) Wien, 30. Jänner 2014 SHORT NOTE 199

deciduous forest of the Estação Ecológica dos Caete- (José Olympio), pp. 450. PANSONATO , A. & M OTT , T. & tus, southeastern Brazil.- Biota Neotropica, Campinas; STRüSSMANN , C. (2011): Anuran amphibians’ diversity 10 (1): 275-292. CALdERON , L. A. & d ELAix -Z AqUEO , in a northwestern area of the Brazilian Pantanal.- Biota K. & Z AqUEO , K. d. & S ERRANO , R. P. & M ESSiAS , M. Neotropica, Campinas; 11 (4): 1-10. PiATTi , L. & R. & C ARdOZO FiLHO , J. L. & d iNiZ -S OUSA , R. & SOUZA , F. L. & L ANdgREF FiLHO , P. (2010): Anuran HOLANdA , R. J. & R EgO T. B. & S TABELi , R. g. (2009): assemblage in a rice field agroecosystem in the Amphibia, Anura, Leptodactylidae, Leptodactylus Pantanal of central Brazil.- Journal of Natural History, chaquensis : distribution extension and geographic dis - London; 44 (19): 1215-1224. PiNHEiRO , L. C. & B iTAR , tribution map.- Check List, São Paulo; 5 (3): 425-427. y. O. C. & g ALATTi , U. & N ECKEL -O LiVEiRA , S. & CAMPOS , J. B. (1999): Spatial and multi-temporal SANTOS -C OSTA , M. C. ( 2012): Amphibians from south - analysis of deforestation and quantification of the rem - eastern state of Pará: Carajás Region, northern Brazil.- nant forests on Porto Rico island, Paraná, Brazil. - Check List, São Paulo; 8 (4): 693–702. PRAdO , C. P. A. Brazilian Archives of Biology and Technology, & H AddAd , C. F. B. (2003): Testes size in leptodactylid Curitiba; 42 (1): 91-100. CAMPOS FiLHO , L. V. (2009): frogs and occurrence of multimale spawning in the Lista de anfíbios e répteis registrados na área do Pouso genus Leptodactylus in Brazil.- Journal of Herpetology, Alegre Hotel-Fazenda, Pantanal de Mato grosso, dallas; 37: 354-362. PRAdO , C. P. A. & H AddAd , C. F. Brasil. WWW document available at < http://www. B. (2005): Size-fecundity relationships and reproduc - pousalegre.com.br/fauna_rept.htm > (last accessed 02. tive investment in female frogs in the Pantanal, south- 2013). dORAdO -R OdRigUES , T. F. & C AMPOS , V. A. & western Brazil.- Herpetological Journal, London; 15: SANTOS , M. M. & P ANSONATO , A. & S TRüSSMANN , C. 181-189. PRAdO , C. P. A. & U ETANABARO , M. & (2012 ): Circumstances and bioacoustics of the distress HAddAd , C. F. B. (2005): Breeding activity patterns, call of Leptodactylus chaquensis (Anura: Leptodacty- reproductive modes, and habitat use by anurans lidae) during predation by Thamnodynastes chaquensis (Amphibia) in a seasonal environment in the Pantanal, (Serpentes: dipsadidae) in the Brazilian Pantanal.- Brazil.- Amphibia-Reptilia, Leiden; 26 (2): 211-221. Salamandra, Frankfurt a. M.; 48 (4): 237-240. FROST , PRAdO , C. P. A. & U ETANABARO , M. & L OPES , F. S. d. R. (2013): Amphibian species of the world: an (2000): Reproductive strategies of Leptodactylus online reference. Version 5.6 (9 January 2013). chaquensis and L. podicipinus in the Pantanal, Brazil.- American Museum of Natural History, New york, Journal of Herpetology, dallas; 34 (1): 135-139. USA. WWW document available at < http://research. SANTOS , M. M. & Á ViLA , R. W. & K AWASHiTA -R iBEiRO , amnh.org/vz/herpetology/amphibia/ > (last accessed R. A. (2011): Checklist of the amphibians and reptiles 02.2013). gARCiA , P. C. A. & V iNCiPROVA , g. (1998): in Nobres Municipality, Mato grosso State, central Range extensions of some anuran species for Santa Brazil.- Herpetology Notes, Calci (Pisa); 4: 455-461. Catarina and Rio grande do Sul states, Brazil.- SANTOS , T. g. & C ECHiN , S. Z. (2008): Amphibia, Herpetological Review, Saint Louis; 29 (2): 117-118. Anura, Leptodactylidae, Lepto dactylus chaquensis : giARETTA , A. A. & M ENiN , M. & F ACURE , K. g. & distribution extension in the State of Rio grande do KOKUBUM , M. N. C. & O LiVEiRA FiLHO , J. C. (2008): Sul, Brazil. - Check List, São Paulo; 4 (2): 142-144. Species richness, relative abundance, and habitat of SANTOS , T. g. & R OSSA -F ERES , d. C. & C ASATTi , L. reproduction of terrestrial frogs in the Triângulo (2007): diversidade e distribuição espaço-temporal de Mineiro region, Cerrado biome, southeastern Brazil.- anuros em região com pronunciada estação seca no iheringia, Porto Alegre; (Série Zoologia) 98: 181-188. sudeste do Brasil.- iheringia, , Porto Alegre; (Série gORdO , M. & C AMPOS , Z. (2005): Anuros das serras de Zoologia) 97 (1): 37-49. SANTOS , T. g. & entorno do Pantanal sul.- Série documentos/Embrapa VASCONCELOS , T. S. & R OSSA -F ERES , d. C. & H AddAd , Pantanal, Corumbá; 78: 1-21. HEyER , W. R. (1969): C. F. B. (2009): Anurans of a seasonally dry tropical The adaptive ecology of the species group of the genus forest: Morro do diabo State Park, São Paulo State, Leptodactylus (Amphibia, Leptodactylidae).- Evol - Brazil.- Journal of Natural History, London; 43 (15): ution, Tempe; 23: 421-428. HEyER , W. R. & dE SÁ, R. 973-993. SiLVEiRA , A. L. (2006): Anfíbios do municí - O. (2011 ): Variation, systematics, and relationships of pio de João Pinheiro, uma área de Cerrado no noroeste the Leptodactylus bolivianus complex (Amphibia: de Minas gerais, Brasil.- Arquivos do Museu Nacional, Anura: Leptodactylidae).- Smithsonian Contributions Rio de Janeiro; 64 (2): 131-139. SOUZA , F. L. & to Zoology, Washington; 635: 1-58. HEyER , W. R. & UETANABARO , M. & L ANdgREF FiLHO , P. & P iATTi , L. & MUñOZ , A. M. (1999): Validation of Eleutherodactylus PRAdO , C. P. A. (2010): Herpetofauna, Municipality of crepitans BOKERMANN , 1965, notes on the types and Porto Murtinho, Chaco region, Mato grosso do Sul type locality of Telatrema [sic] heterodactylum State, Brazil.- Check List, São Paulo; 6 (3): 470-475. MiRANdA -R iBEiRO , 1937, and description of a new SOUZA , M. B. (2009): Anfíbios: Reserva Extrativista do species of Eleutherodactylus from Mato grosso, Brazil Alto Juruá e Parque Nacional da Serra do divisor, (Amphibia: Anura: Leptodactylidae).- Proceedings of Acre. Campinas (iFCH), pp. 77. STRüSSMANN , C. the Biological Society of Washington, Washington; (2000): Herpeto fauna; pp. 153-189. in: ALHO , C. J. R. 112: 1-18. HEyER , H. & R EiCHLE , S. & S iLVANO , d. & (Coord.): Fauna silvestre da região do Rio Manso – LAViLLA , E. & di TAdA , i. (2004): Leptodactylus MT; Brasília (Ministério do Meio Ambiente, Edições chaquensis . in iUCN 2012. iUCN Red List of iBAMA, Centrais Elétricas do Norte do Brasil). Threatened Species. Version 2012.2. WWW document STRüSSMANN , C. & P RAdO , C. P. A. & U ETANABARO , M. available at < www.iucnredlist.org > (last accessed: 28 & F ERREiRA , V. L. (2000): Appendix 11 - Anuran February 2013). LUCAS , E. M. & M A ROCCO , J. C. species recorded in each region. Apêndice 11 - (2011): Anurofauna (Amphibia, Anura) em um Espécies de anuros registradas em cada região; pp. remanescente de floresta ombrófila mista no Estado de 303-304. in: WiLLiNK , P. W. & C HERNOFF , B. & Santa Catarina, Sul do Brasil.- Biota Neotropica, ALONSO , L. E. & M ONTAMBAULT , J. R. & L OURiVAL , R. Campinas; 11 (1): 377-384. MAACK , R. (1981): (eds.): RAP Bulletin of Biological Assessment, 18. A geografia física do Estado do Paraná; Rio de Janeiro biological assessment of the aquatic ecosystems of the 200 SHORT NOTE HERPETOZOA 26 (3/4) Wien, 30. Jänner 2014 SHORT NOTE

Pantanal, Mato grosso do Sul, Brasil. Uma avaliação Using micro-histology and an biológica dos ecossistemas aquáticos do Pantanal, Mato grosso do Sul, Brasil; Washington (Conservation image-based identification tool international). TRiNdAdE , A. O. & O LiVEiRA , S. V. & to study the diet of Iguana iguana CAPPELLARi , L. H. (2010): Anfíbios anuros de uma área da Serra do Sudeste, Rio grande do Sul (Caçapava do (LiNNAEUS , 1758) Sul).- Biodiversidade Pampeana, Uruguaiana; 8 (1): 19-24. UETANABARO , M. & P RAdO , C. P. A. & ROdRigUES , d. J. & g ORdO , M. & C AMPOS , Z. (2008): The adult green iguana, Iguana igua - guia de campo dos anuros do Pantanal Sul e Planaltos na (LiNNAEUS , 1758), is a Neotropical arbo - de Entorno; Campo grande, MS (Editora UFMS), real herbivore according to studies based on Cuiabá (Ed. UFMT), pp. 196. UETANABARO , M. & stomach contents, fecal samples and direct SOUZA , F. L. & L ANdgREF FiLHO , P. & B EdA , A. F. & VERSON ANd BRANdÃO , R. A. (2007): Anfíbios e répteis do Parque observations ( i 1982; R et al. Nacional da Serra da Bodoquena, Mato grosso do Sul, 1990). This species represents one out of Brasil.- Biota Neotropica, Campinas; 7 (3): 279-289. about 50 truly herbivorous lizard species VAiRA , M. & A KMENTiNS , M. S. & A TTAdEMO , M. & worldwide ( MACKiE et al 2004) and rarely BALdO , d. & B ARRASSO , d. & B ARRiONUEVO , S. & BASSO , N. & B LOTTO , B. & C AiRO , S. & C AJAdE , R. & eats animal material such as tree snails, bird CéSPEdEZ , J. & C ORBALÁN , V. & C HiLOTE , P. & d URé , eggs, chicks and small mammals ( TOWNS- M. & F ALCiONE , C. & F ERRARO , d. & g UTiERREZ , F. R. ENd et al. 2005; LóPEZ -T ORRES et al. 2012). & i NgARAMO , M. R. & J UNgES , C. & L AJMANOViCH , R. Iguana is an ectothermic hindgut fermenter & L ESCANO , J. N. & M ARANgONi , F. & M ARTiNAZZO , ACKiE L. & M ARTi , R. & M ORENO , L. & N ATALE , g. S. & using gut microbes to digest food ( M PEREZ igLESiAS , J. M. & P ELTZER , P. & q UiROgA , L. & et al. 2004). in that green iguanas eat ROSSET , S. & S ANABRiA , E. & S ANCHEZ , L. & seeds, they play an important role in the SCHAEFER , E. & ú BEdA , C. & Z ARACHO , V. (2012): reproductive strategies of both native and Categorización del estado de conservación de los anfi- bios de la República Argentina.- Cuadernos de Herpe - non-native plants throughout their range tología, Buenos Aires; 26 (Suppl. 1): 131-159. VALéRiO - (BENíTEZ -M ALVAd et al. 2003; gUZMÁN BRUN , L. M. & P ANSONATO , A. & S OLiNO -C ARVALHO , L. 2007 ). To understand this ecological role of A. & S TRüSSMANN , C. & M OTT , T. & S iLVEiRA , R. M. green iguanas, plants consumed by the L. (2010): Sapos, rãs e pererecas; pp. 119-135. in: FER - NANdES , i. M. & S igNOR , C. A. & P ENHA , J. (eds.): species needed to be identified. despite this Biodiversidade no Pantanal de Poconé; Manaus (Átem - lizard’s wide distribution throughout Brazil, ma). VASCONCELOS , T. S. & R OSSA -F ERES , d. C. ongoing studies focus mainly on its repro - (2005): diversidade, distribuição espacial e temporal duction ( FERREiRA et al. 2002; CAMPOS de anfíbios anuros (Amphibia, Anura) na região nor- oeste do estado de São Paulo, Brasil.- Biota Neotropi- 2004; CAMPOS & d ES BiEZ 201 3) and most ca, Campinas; 5 (2): 1-14. recently coprophagy behavior ( CAMPOS et KEy WORdS: Amphibia: Anura, Leptodacty- al. 2010), but not diet. lidae, Leptodactylus latrans group, Leptodactylus The purpose of this study was to test chaquensis , new record, Paraná, Brazil methods for the identification of plant SUBMiTTEd: March 21, 2013 species consumed by free ranging green AUTHORS: Fabrício H. OdA (Corresponding iguanas without killing the lizards. For this author < [email protected] >) 1) ; danusy L. purpose, the technique of micro-histological SANTOS 2) ; Priscilla g. gAMBALE 1) ; Vitor A. CAMPOS 3) ; Vinicius g. BATiSTA 1) ; igor P. AFFONSO 1) ; Christine image analysis was applied to determine the STRüSSMANN 4) botanical composition of plant materials 1) Núcleo de Pesquisas em Limnologia, ictio logia found in the stomachs and faecal samples. e Aqüicultura (Nupélia), Uni versidade Estadual de This technique is widely used to examine Maringá (UEM). Av. Colombo, 5790, Bloco g-90, CEP 87020-900, Maringá, Paraná, Brazil. the diet of mammals ( SANTOS et al. 2002; 2) Laboratório de Herpetologia e Comporta- dESBiEZ et al. 2011) but was never adopted mento Animal, Universidade Federal de goiás (UFg). to green iguanas. Rodovia goiânia-Nerópolis, km 5, Setor itatiaia, CEP The present study was conducted in the 74001-970, Nerópolis, goiás, Brazil. Private Reserve of Natural Heritage (PRNP) 3) instituto de Biosciências, Universidade Fede- ral de Mato grosso (UFMT). Av. Fernando Corrêa da - SESC Pantanal (center: 16°31.636’S / 56° Costa, s/n, CCBS-ii, Boa Esperança, CEP 78060-900, 22.772’W), in the Barão de Melgaço sub- Cuiabá, Mato grosso, Brazil. region, Pantanal, Brazil. in 2008 and 2009, 4) Universidade Federal de Mato grosso, Facul - green iguanas were located during night dade de Agronomia, Medicina Veterinária e Zootecnia, departamento de Ciências Básicas e Produção Animal, surveys while sleeping on branches of trees Av. Fernando Correia, 2367, Boa Esperança, CEP and shrubs on the banks of the Cuiabá River 78060-900. Cuiabá, Mato grosso, Brazil. through the use of a sealed-beam light con -