cells Review Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research Lei Chen 1 , Shuncong Wang 1 , Yuanbo Feng 1, Jinyong Zhang 2,3, Yuqing Du 2, Jiang Zhang 4, Chantal Van Ongeval 1, Yicheng Ni 1,* and Yue Li 2,* 1 KU Leuven, Biomedical Group, Campus Gasthuisberg, 3000 Leuven, Belgium;
[email protected] (L.C.);
[email protected] (S.W.);
[email protected] (Y.F.);
[email protected] (C.V.O.) 2 Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China;
[email protected] (J.Z.);
[email protected] (Y.D.) 3 School of Medical Instrument and Food Engineering, University of Shanghai for Science & Technology, Shanghai 200093, China 4 Faculty of Agricultural Biotechnology and Ecotechnology, Shanghai Vocational College of Agriculture and Forestry, Shanghai 201600, China;
[email protected] * Correspondence:
[email protected] (Y.N.);
[email protected] (Y.L.) Abstract: The fertilised chick egg and particularly its chorioallantoic membrane (CAM) have drawn continuing interest in biomedicine and bioengineering fields, especially for research on vascular study, cancer, drug screening and development, cell factors, stem cells, etc. This literature review sys- temically introduces the CAM’s structural evolution, functions, vascular features and the circulation system, and cell regulatory factors. It also presents the major and updated applications of the CAM in assays for pharmacokinetics and biodistribution, drug efficacy and toxicology testing/screening in preclinical pharmacological research. The time course of CAM applications for different assays Citation: Chen, L.; Wang, S.; Feng, Y.; and their advantages and limitations are summarised.