1 the Evolution of Endocrine

Total Page:16

File Type:pdf, Size:1020Kb

1 the Evolution of Endocrine THE EVOLUTION OF ENDOCRINE EXTRAEMBRYONIC MEMBRANES; A COMPARATIVE STUDY OF STEROIDOGENESIS AND STEROID SIGNALING IN THE CHORIOALLANTOIC MEMBRANE OF OVIPAROUS AMNIOTES By LORI CRUZE ALBERGOTTI A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2011 1 © 2011 Lori Cruze Albergotti 2 Ken, I will be forever grateful for your love and support. 3 ACKNOWLEDGMENTS First, I would like to thank Lou Guillette for his generous financial, technical and emotional support. Lou has taught me that being a scientist is the opportunity to be a detective in pursuit of answers to an intriguing question, and this pursuit requires not only an analytical mind, but also a creative one. I thank Lou for giving me this opportunity and for his endless guidance and encouragement, and last but not least for his friendship. I could not have asked for a better graduate experience and I am convinced that this has everything to do with having Lou as an advisor and Bernie, Colette, Marty and Malcolm as committee members. I would like to thank Bernie for pushing me outside of my comfort zone, for encouraging me to think broadly about my research, and for his willingness to provide lab assistance. Thank you to Colette for lending statistical and experimental design expertise, for pushing me to think about the big picture of my research, and for always believing in me. I would like to thank Marty for expanding my understanding of evolution and development, for allowing me the amazing opportunity to teach with him, and for making his lab available to me. Thank you to Malcolm for his expertise in developmental signaling, for his constant enthusiasm, and for his willingness to the take time to hear how things are going and offer words of encouragement. I would also like to thank the Department of Biology for providing a wonderful academic home for the last five years. I would like to thank the Biology faculty in general for being excellent academic role models and in particular I would like to thank Rebecca Kimball, Ed Braun, Charlie Baer, Marta Wayne and Dave Evans for taking an interest in me, offering guidance and assistance, and for helping me become a better scientist. I would also like to thank Keith Choe for the generous use of his microscope and camera. 4 Thank you to my lab mates, colleagues and friends from whom I have learned so much. Thanks to Satomi Kohno and Brandon Moore for teaching me molecular biology, for providing technical advice, and for helping me hit the ground running. Thank you to Thea Edwards and Alison Roark for sharing my interest in the placenta and extraembryonic membranes and for the countless hours of conversation that helped to define my ideas about the evolution of these structures. Thank you to Heather Hamlin for her assistance with tissue culture and hormone techniques, for her boundless energy and amazing spirit. Thank you to Krista McCoy, Leslie Babonis, Kelly Hyndman, and Marda Jorgensen for providing histology, immunohistochemistry and microscopy training and advice. Thank you to Mike McCoy for providing statistical expertise and support and for pushing me to think outside the box about data analysis. Thank you to Ashley Boggs and Nicole Botteri for sharing this experience with me and for their continuous support and friendship. Thanks to the wonderful undergraduate students that I have had the pleasure to mentor and without whose toiling hours in the lab this work would have been incredibly difficult; Chris Olmo, Yao Fu, Sasha Strul, Jacob Fyda, Shellah Palmer, Jenna Harty, Momna Younas, Gabrielle Rolland, and Patpilai Kasinpila. I would also like to thank the Florida Fish and Wildlife Conservation Commission for their assistance in the collection of alligator and turtle eggs. Finally, I would like to thank my mom and dad for their love and encouragement. A special thanks to all of my friends, but especially Chip, Michelle, Jon, Christine, Stephanie and the EC, for keeping me sane these last few years. I thank my wonderful husband for making me laugh every day, for reminding me that life is a balance and for his willingness to join in this adventure with me. 5 TABLE OF CONTENTS page ACKNOWLEDGMENTS .................................................................................................. 4 LIST OF TABLES ............................................................................................................ 9 LIST OF FIGURES ........................................................................................................ 10 LIST OF ABBREVIATIONS ........................................................................................... 12 ABSTRACT ................................................................................................................... 16 CHAPTER 1 INTRODUCTION .................................................................................................... 19 The Evolution of Viviparity ...................................................................................... 20 Egg Retention and the Lengthening of Gestation ............................................. 21 The Reduction of the Eggshell ......................................................................... 24 The Emergence of the Placenta ....................................................................... 27 An Endocrine Placenta ........................................................................................... 30 A New Hypothesis for the Evolution of Endocrine Extraembryonic Membranes and the Placenta: Implications for the Evolution of Viviparity ............................... 32 Specific Hypotheses ............................................................................................... 33 Significance of Work ............................................................................................... 34 2 STEROIDOGENESIS AND STEROID SIGNALING IN THE CHORIOALLANTOIC MEMBRANE OF THE CHICKEN (GALLUS GALLUS) ........ 37 Materials and Methods............................................................................................ 39 CAM Collection ................................................................................................. 39 RNA Isolation and Reverse Transcription......................................................... 39 Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) ...................... 39 Cloning and Sequencing of Plasmids ............................................................... 41 RT–qPCR Primers ............................................................................................ 41 Sexing of Embryos ........................................................................................... 41 In Vitro Explant Culture .................................................................................... 42 Immunohistochemistry and Microscopy............................................................ 42 Statistical Analysis ............................................................................................ 43 Results .................................................................................................................... 45 The Chicken CAM has the Required Molecular Mechanisms to Perform Steroidogenesis and Synthesis of Progesterone ........................................... 45 The Chicken CAM is Capable of In Vitro Progesterone Synthesis ................... 46 The Chicken CAM is Capable of Responding to P4 Signaling Through the Progesterone Receptor ................................................................................. 47 6 Discussion .............................................................................................................. 49 3 THE CHORIOALLANTOIC MEMBRANE OF THE AMERICAN ALLIGATOR (ALLIGATOR MISSISSIPPIENSIS) HAS THE CAPABILITY TO PERFORM STEROID BIOSYNTHESIS AND RESPOND TO STEROID HORMONE SIGNALING ............................................................................................................ 56 Materials and Methods............................................................................................ 60 Egg Collection and Sample Preparation ........................................................... 60 RNA Isolation and Reverse Transcription......................................................... 61 Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) ...................... 61 RT-qPCR Normalization ................................................................................... 62 Cloning and Sequencing of Plasmids ............................................................... 63 Immunohistochemistry and Microscopy............................................................ 63 Statistical Analysis ............................................................................................ 64 Results .................................................................................................................... 66 Internal Control Expression among Embryonic Stages and between Incubation Temperatures .............................................................................. 66 Steroidogenic Factor, Enzyme and Steroid Receptor Expression among Embryonic Stages ......................................................................................... 67 Steroidogenic Factor, Enzyme and Steroid Receptor Expression between Incubation Temperatures .............................................................................
Recommended publications
  • A Record of Spencer's Skink Pseudemoia Spenceri from The
    Contributions A record of Spencer’s Skink Pseudemoia spenceri from the Victorian Volcanic Plain Peter Homan School of Life & Physical Sciences, RMIT University, GPO Box 2476V, Melbourne, Victoria 3001. Email: [email protected] Abstract During a survey of vertebrate fauna at a site in Yan Yean, north of Melbourne on the Victorian Volcanic Plain, a small population of Spencer’s Skink Pseudemoia spenceri was found inhabiting a heritage dry stone fence. Spencer’s Skink is normally found in wet schlerophyll forest and cool temperate environments, and the species is not considered a grassland inhabitant. There are no other records of Spencer’s Skink occurring in any part of the Victorian Volcanic Plain. (The Victorian Naturalist 128(3) 2011, 106-110) Keywords: Spencer’s Skink Pseudemoia spenceri, Volcanic Plain, grasslands, dry stone fences. Introduction The Growling Frog Golf Course (GFGC) is the dry stone fences as habitat. These include situated on the Victorian Volcanic Plain in Yan Large Striped Skink Ctenotus robustus, Bou- Yean (37° 33'S, 145° 04'E), approximately 33 km gainville’s Skink Lerista bougainvillii, Lowland north-north-east of the Melbourne Central Copperhead Austrelaps superbus, Little Whip Business District. The course was established Snake Parasuta flagellum, Southern Bullfrog in 2005 by the City of Whittlesea under strict Limnodynastes dumerilii and Spotted Marsh environmental conditions that required the Frog Limnodynastes tasmaniensis. preservation of important natural and herit- Record of Spencer’s Skink Pseudemoia spen- age features. These included protection of ceri inhabiting dry stone fence stony knolls, ephemeral wetlands and an area On 26 March 2010, staff and students from the of Plains Grassy Woodland; preservation of all School of Life and Physical Sciences, RMIT River Red Gums Eucalyptus camaldulensis and University, visited the GFGC to examine a hab- several rare plant species; and retention of her- itat enhancement program near the dry stone itage dry stone fences.
    [Show full text]
  • Herpetological Information Service No
    Type Descriptions and Type Publications OF HoBART M. Smith, 1933 through June 1999 Ernest A. Liner Houma, Louisiana smithsonian herpetological information service no. 127 2000 SMITHSONIAN HERPETOLOGICAL INFORMATION SERVICE The SHIS series publishes and distributes translations, bibliographies, indices, and similar items judged useful to individuals interested in the biology of amphibians and reptiles, but unlikely to be published in the normal technical journals. Single copies are distributed free to interested individuals. Libraries, herpetological associations, and research laboratories are invited to exchange their publications with the Division of Amphibians and Reptiles. We wish to encourage individuals to share their bibliographies, translations, etc. with other herpetologists through the SHIS series. If you have such items please contact George Zug for instructions on preparation and submission. Contributors receive 50 free copies. Please address all requests for copies and inquiries to George Zug, Division of Amphibians and Reptiles, National Museum of Natural History, Smithsonian Institution, Washington DC 20560 USA. Please include a self-addressed mailing label with requests. Introduction Hobart M. Smith is one of herpetology's most prolific autiiors. As of 30 June 1999, he authored or co-authored 1367 publications covering a range of scholarly and popular papers dealing with such diverse subjects as taxonomy, life history, geographical distribution, checklists, nomenclatural problems, bibliographies, herpetological coins, anatomy, comparative anatomy textbooks, pet books, book reviews, abstracts, encyclopedia entries, prefaces and forwords as well as updating volumes being repnnted. The checklists of the herpetofauna of Mexico authored with Dr. Edward H. Taylor are legendary as is the Synopsis of the Herpetofalhva of Mexico coauthored with his late wife, Rozella B.
    [Show full text]
  • Xenosaurus Tzacualtipantecus. the Zacualtipán Knob-Scaled Lizard Is Endemic to the Sierra Madre Oriental of Eastern Mexico
    Xenosaurus tzacualtipantecus. The Zacualtipán knob-scaled lizard is endemic to the Sierra Madre Oriental of eastern Mexico. This medium-large lizard (female holotype measures 188 mm in total length) is known only from the vicinity of the type locality in eastern Hidalgo, at an elevation of 1,900 m in pine-oak forest, and a nearby locality at 2,000 m in northern Veracruz (Woolrich- Piña and Smith 2012). Xenosaurus tzacualtipantecus is thought to belong to the northern clade of the genus, which also contains X. newmanorum and X. platyceps (Bhullar 2011). As with its congeners, X. tzacualtipantecus is an inhabitant of crevices in limestone rocks. This species consumes beetles and lepidopteran larvae and gives birth to living young. The habitat of this lizard in the vicinity of the type locality is being deforested, and people in nearby towns have created an open garbage dump in this area. We determined its EVS as 17, in the middle of the high vulnerability category (see text for explanation), and its status by the IUCN and SEMAR- NAT presently are undetermined. This newly described endemic species is one of nine known species in the monogeneric family Xenosauridae, which is endemic to northern Mesoamerica (Mexico from Tamaulipas to Chiapas and into the montane portions of Alta Verapaz, Guatemala). All but one of these nine species is endemic to Mexico. Photo by Christian Berriozabal-Islas. amphibian-reptile-conservation.org 01 June 2013 | Volume 7 | Number 1 | e61 Copyright: © 2013 Wilson et al. This is an open-access article distributed under the terms of the Creative Com- mons Attribution–NonCommercial–NoDerivs 3.0 Unported License, which permits unrestricted use for non-com- Amphibian & Reptile Conservation 7(1): 1–47.
    [Show full text]
  • Mechanism of Wound-Healing Activity of Hippophae Rhamnoides L. Leaf Extract in Experimental Burns
    Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 659705, 9 pages doi:10.1093/ecam/nep189 Original Article Mechanism of Wound-Healing Activity of Hippophae rhamnoides L. Leaf Extract in Experimental Burns Nitin K. Upadhyay,1 Ratan Kumar,1 M. S. Siddiqui,2 and Asheesh Gupta1 1 Defence Institute of Physiology and Allied Science, DRDO, Delhi 110054, India 2 Department of Toxicology, Jamia Hamdard, Delhi 110062, India Correspondence should be addressed to Asheesh Gupta, [email protected] Received 20 July 2009; Accepted 19 October 2009 Copyright © 2011 Nitin K. Upadhyay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoides L., family Elaeagnaceae) (SBT) and to explore its possible mechanism of action on experimental burn wounds in rats. The SBT extract, at various concentrations, was applied topically, twice daily for 7 days. Treatment with silver sulfadiazine (SSD) ointment was used as reference control. The most effective concentration of the extract was found to be 5.0% (w/w) for burn wound healing and this was further used for detailed study. The SBT-treated group showed faster reduction in wound area in comparison with control and SSD-treated groups. The topical application of SBT increased collagen synthesis and stabilization at the wound site, as evidenced by increase in hydroxyproline, hexosamine levels and up-regulated expression of collagen type-III.
    [Show full text]
  • Amphibian Alliance for Zero Extinction Sites in Chiapas and Oaxaca
    Amphibian Alliance for Zero Extinction Sites in Chiapas and Oaxaca John F. Lamoreux, Meghan W. McKnight, and Rodolfo Cabrera Hernandez Occasional Paper of the IUCN Species Survival Commission No. 53 Amphibian Alliance for Zero Extinction Sites in Chiapas and Oaxaca John F. Lamoreux, Meghan W. McKnight, and Rodolfo Cabrera Hernandez Occasional Paper of the IUCN Species Survival Commission No. 53 The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of IUCN concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of IUCN or other participating organizations. Published by: IUCN, Gland, Switzerland Copyright: © 2015 International Union for Conservation of Nature and Natural Resources Reproduction of this publication for educational or other non-commercial purposes is authorized without prior written permission from the copyright holder provided the source is fully acknowledged. Reproduction of this publication for resale or other commercial purposes is prohibited without prior written permission of the copyright holder. Citation: Lamoreux, J. F., McKnight, M. W., and R. Cabrera Hernandez (2015). Amphibian Alliance for Zero Extinction Sites in Chiapas and Oaxaca. Gland, Switzerland: IUCN. xxiv + 320pp. ISBN: 978-2-8317-1717-3 DOI: 10.2305/IUCN.CH.2015.SSC-OP.53.en Cover photographs: Totontepec landscape; new Plectrohyla species, Ixalotriton niger, Concepción Pápalo, Thorius minutissimus, Craugastor pozo (panels, left to right) Back cover photograph: Collecting in Chamula, Chiapas Photo credits: The cover photographs were taken by the authors under grant agreements with the two main project funders: NGS and CEPF.
    [Show full text]
  • REPTILIA: SQUAMATA: PHRYNOSOMATIDAE Sceloporus Poinsettii
    856.1 REPTILIA: SQUAMATA: PHRYNOSOMATIDAE Sceloporus poinsettii Catalogue of American Amphibians and Reptiles. Webb, R.G. 2008. Sceloporus poinsettii. Sceloporus poinsettii Baird and Girard Crevice Spiny Lizard Sceloporus poinsettii Baird and Girard 1852:126. Type-locality, “Rio San Pedro of the Rio Grande del Norte, and the province of Sonora,” restricted to either the southern part of the Big Burro Moun- tains or the vicinity of Santa Rita, Grant County, New Mexico by Webb (1988). Lectotype, National Figure 1. Adult male Sceloporus poinsettii poinsettii (UTEP Museum of Natural History (USNM) 2952 (subse- 8714) from the Magdalena Mountains, Socorro County, quently recataloged as USNM 292580), adult New Mexico (photo by author). male, collected by John H. Clark in company with Col. James D. Graham during his tenure with the U.S.-Mexican Boundary Commission in late Au- gust 1851 (examined by author). See Remarks. Sceloporus poinsetii: Duméril 1858:547. Lapsus. Tropidolepis poinsetti: Dugès 1869:143. Invalid emendation (see Remarks). Sceloporus torquatus Var. C.: Bocourt 1874:173. Sceloporus poinsetti: Yarrow “1882"[1883]:58. Invalid emendation. S.[celoporus] t.[orquatus] poinsettii: Cope 1885:402. Seloporus poinsettiii: Herrick, Terry, and Herrick 1899:123. Lapsus. Sceloporus torquatus poinsetti: Brown 1903:546. Sceloporus poissetti: Král 1969:187. Lapsus. Figure 2. Adult female Sceloporus poinsettii axtelli (UTEP S.[celoporus] poinssetti: Méndez-De la Cruz and Gu- 11510) from Alamo Mountain (Cornudas Mountains), tiérrez-Mayén 1991:2. Lapsus. Otero County, New Mexico (photo by author). Scelophorus poinsettii: Cloud, Mallouf, Mercado-Al- linger, Hoyt, Kenmotsu, Sanchez, and Madrid 1994:119. Lapsus. Sceloporus poinsetti aureolus: Auth, Smith, Brown, and Lintz 2000:72.
    [Show full text]
  • Expert Report of Professor Woinarski
    NOTICE OF FILING This document was lodged electronically in the FEDERAL COURT OF AUSTRALIA (FCA) on 18/01/2019 3:23:32 PM AEDT and has been accepted for filing under the Court’s Rules. Details of filing follow and important additional information about these are set out below. Details of Filing Document Lodged: Expert Report File Number: VID1228/2017 File Title: FRIENDS OF LEADBEATER'S POSSUM INC v VICFORESTS Registry: VICTORIA REGISTRY - FEDERAL COURT OF AUSTRALIA Dated: 18/01/2019 3:23:39 PM AEDT Registrar Important Information As required by the Court’s Rules, this Notice has been inserted as the first page of the document which has been accepted for electronic filing. It is now taken to be part of that document for the purposes of the proceeding in the Court and contains important information for all parties to that proceeding. It must be included in the document served on each of those parties. The date and time of lodgment also shown above are the date and time that the document was received by the Court. Under the Court’s Rules the date of filing of the document is the day it was lodged (if that is a business day for the Registry which accepts it and the document was received by 4.30 pm local time at that Registry) or otherwise the next working day for that Registry. No. VID 1228 of 2017 Federal Court of Australia District Registry: Victoria Division: ACLHR FRIENDS OF LEADBEATER’S POSSUM INC Applicant VICFORESTS Respondent EXPERT REPORT OF PROFESSOR JOHN CASIMIR ZICHY WOINARSKI Contents: 1.
    [Show full text]
  • Literature Cited in Lizards Natural History Database
    Literature Cited in Lizards Natural History database Abdala, C. S., A. S. Quinteros, and R. E. Espinoza. 2008. Two new species of Liolaemus (Iguania: Liolaemidae) from the puna of northwestern Argentina. Herpetologica 64:458-471. Abdala, C. S., D. Baldo, R. A. Juárez, and R. E. Espinoza. 2016. The first parthenogenetic pleurodont Iguanian: a new all-female Liolaemus (Squamata: Liolaemidae) from western Argentina. Copeia 104:487-497. Abdala, C. S., J. C. Acosta, M. R. Cabrera, H. J. Villaviciencio, and J. Marinero. 2009. A new Andean Liolaemus of the L. montanus series (Squamata: Iguania: Liolaemidae) from western Argentina. South American Journal of Herpetology 4:91-102. Abdala, C. S., J. L. Acosta, J. C. Acosta, B. B. Alvarez, F. Arias, L. J. Avila, . S. M. Zalba. 2012. Categorización del estado de conservación de las lagartijas y anfisbenas de la República Argentina. Cuadernos de Herpetologia 26 (Suppl. 1):215-248. Abell, A. J. 1999. Male-female spacing patterns in the lizard, Sceloporus virgatus. Amphibia-Reptilia 20:185-194. Abts, M. L. 1987. Environment and variation in life history traits of the Chuckwalla, Sauromalus obesus. Ecological Monographs 57:215-232. Achaval, F., and A. Olmos. 2003. Anfibios y reptiles del Uruguay. Montevideo, Uruguay: Facultad de Ciencias. Achaval, F., and A. Olmos. 2007. Anfibio y reptiles del Uruguay, 3rd edn. Montevideo, Uruguay: Serie Fauna 1. Ackermann, T. 2006. Schreibers Glatkopfleguan Leiocephalus schreibersii. Munich, Germany: Natur und Tier. Ackley, J. W., P. J. Muelleman, R. E. Carter, R. W. Henderson, and R. Powell. 2009. A rapid assessment of herpetofaunal diversity in variously altered habitats on Dominica.
    [Show full text]
  • IMPACTS of the UNPRECEDENTED 2019-20 BUSHFIRES on AUSTRALIAN ANIMALS NOVEMBER 2020 Acknowledgements
    AUSTRALIA IMPACTS OF THE UNPRECEDENTED 2019-20 BUSHFIRES ON AUSTRALIAN ANIMALS NOVEMBER 2020 Acknowledgements WWF-Australia acknowledges the Traditional Owners of the land on which we work and their continuing connection to their lands, waters, and culture. We pay our respects to Elders – past and present, and their emerging leaders. WWF-Australia is part of the world’s largest conservation network. WWF-Australia has been working to create a world where people live in harmony with nature since 1978. WWF’s mission is to stop the degradation of the Earth’s CONTENTS natural environment and to build a future in which humans live in harmony with nature, by conserving the world’s biological diversity, ensuring that the use of renewable natural resources is sustainable, and promoting the EXECUTIVE SUMMARY 6 reduction of pollution and wasteful consumption. Prepared by Lily M van Eeden, Dale Nimmo, Michael BACKGROUND 10 Mahony, Kerryn Herman, Glenn Ehmke, Joris Driessen, James O’Connor, Gilad Bino, Martin Taylor and Chris 1.1 Fire in Australia 10 Dickman for WWF-Australia 1.2 The 2019-20 bushfire season 10 We are grateful to the researchers who provided data or feedback on the report. These include: 1.3 Scope of this study 12 • Eddy Cannella 1.3.1 Taxa included 14 • David Chapple 1.3.2 Study area 14 • Hugh Davies • Deanna Duffy 1.4 Limitations 17 • Hugh Ford • Chris Johnson 1. MAMMALS 18 • Brad Law 2.1 Methods 18 • Sarah Legge • David Lindenmayer 2.1.1 Most mammals 18 • Simon McDonald 2.1.2 Koalas 19 • Damian Michael 2.2 Results 22 • Harry Moore • Stewart Nichol 2.3 Caveats 22 • Alyson Stobo-Wilson • Reid Tingley 2.
    [Show full text]
  • The Victorian Naturalist
    The Volume 128 (3) June 2011 Published by The Field Naturalists Club of Victoria since 1884 From the Editors Over the long history of The Victorian Naturalist the journal has continued to provide a record of studies by both scientifically-trained and amateur researchers of what was observed at a given time and place. These records have often provided a valuable basis, through comparison, for observing change over time in aspects of natural history. The current issue maintains these traditions, with the papers illustrating such changes. We publish here the first study of the decapods of the Pilliga Scrub in New South Wales, details of an extension of the Victorian range of a species of skink, and observations on an undescribed species of fungi. The range of subject matter in these papers also highlights, once again, the diversity that exists of both interest and study regarding the natural world. The Victorian Naturalist is published six times per year by the Field Naturalists Club of Victoria Inc Registered Office: FNCV, 1 Gardenia Street, Blackburn, Victoria 3130, Australia. Postal Address: FNCV, Locked Bag 3, Blackburn, Victoria 3130, Australia. Phone/Fax (03) 9877 9860; International Phone/Fax 61 3 9877 9860. email: [email protected] www.fncv.org.au Patron: His Excellency, the Governor of Victoria Address correspondence to: The Editors, The Victorian Naturalist, Locked Bag 3, Blackburn, Victoria, Australia 3130. Phone: (03) 9877 9860. Email: [email protected] The opinions expressed in papers and book reviews published in The Victorian Naturalist are those of the authors and do not necessarily represent the views of the FNCV.
    [Show full text]
  • Frogs & Reptiles NE Vic 2018 Online
    Reptiles and Frogs of North East Victoria An Identication and Conservation Guide Victorian Conservation Status (DELWP Advisory List) cr critically endangered en endangered Reptiles & Frogs vu vulnerable nt near threatened dd data deficient L Listed under the Flora and Fauna Guarantee Act (FFG, 1988) Size: of North East Victoria Lizards, Dragons & Skinks: Snout-vent length (cm) Snakes, Goannas: Total length (cm) An Identification and Conservation Guide Lowland Copperhead Highland Copperhead Carpet Python Gray's Blind Snake Nobbi Dragon Bearded Dragon Ragged Snake-eyed Skink Large Striped Skink Frogs: Snout-vent length male - M (mm) Snout-vent length female - F (mm) Austrelaps superbus 170 (NC) Austrelaps ramsayi 115 (PR) Morelia spilota metcalfei – en L 240 (DM) Ramphotyphlops nigrescens 38 (PR) Diporiphora nobbi 8.4 (PR) Pogona barbata – vu 25 (DM) Cryptoblepharus pannosus Snout-Vent 3.5 (DM) Ctenotus robustus Snout-Vent 12 (DM) Guide to symbols Venomous Lifeform F Fossorial (burrows underground) T Terrestrial Reptiles & Frogs SA Semi Arboreal R Rock-dwelling Habitat Type Alpine Bog Montane Forests Alpine Grassland/Woodland Lowland Grassland/Woodland White-lipped Snake Tiger Snake Woodland Blind Snake Olive Legless Lizard Mountain Dragon Marbled Gecko Copper-tailed Skink Alpine She-oak Skink Drysdalia coronoides 40 (PR) Notechis scutatus 200 (NC) Ramphotyphlops proximus – nt 50 (DM) Delma inornata 13 (DM) Rankinia diemensis Snout-Vent 7.5 (NC) Christinus marmoratus Snout-Vent 7 (PR) Ctenotus taeniolatus Snout-Vent 8 (DM) Cyclodomorphus praealtus
    [Show full text]
  • Multilocus Species Delimitation in the Crotalus Triseriatus Species Group (Serpentes: Viperidae: Crotalinae), with the Description of Two New Species
    Zootaxa 3826 (3): 475–496 ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ Article ZOOTAXA Copyright © 2014 Magnolia Press ISSN 1175-5334 (online edition) http://dx.doi.org/10.11646/zootaxa.3826.3.3 http://zoobank.org/urn:lsid:zoobank.org:pub:8D8FCB6B-E1DC-4A00-9257-BCAB7D06AE22 Multilocus species delimitation in the Crotalus triseriatus species group (Serpentes: Viperidae: Crotalinae), with the description of two new species ROBERT W. BRYSON, JR.1,8, CHARLES W. LINKEM1, MICHAEL E. DORCAS2, AMY LATHROP3, JASON M. JONES4, JAVIER ALVARADO-DÍAZ5, CHRISTOPH I. GRÜNWALD6 & ROBERT W. MURPHY3,7 1Department of Biology & Burke Museum of Natural History and Culture, University of Washington, Box 351800, Seattle, WA 98195- 1800, USA. E-mail: [email protected] 2Department of Biology, Davidson College, Davidson, NC 28035-7118, USA. E-mail: [email protected] 3Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, Toronto, Ontario, Canada M5S 2C6. E-mail: [email protected]; [email protected] 416310 Avenida Florencia, Poway, California 92064, USA. E-mail: [email protected] 5Instituto de Investigaciones sobre los Recursos Naturales, Universidad Michoacana de San Nicolás de Hidalgo, Av. San Juanito Itzic- uaro s/n, Col. Nueva Esperanza, C.P. 58337, Morelia, Michoacán, México. 6Carretera Chapala-Jocotepec Oriente #57-1, Col. Centro, C.P. 45920, Ajijic, Jalisco, México. E-mail: [email protected] 7State Key Laboratory of Genetic Resources and Evolution, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kun- ming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming 250223, Yunnan, China. E-mail: [email protected] 8Corresponding author.
    [Show full text]