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Normal Tissue Controls for Immunohistochemistry

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Normal Tissue Controls for Immunohistochemistry Technical Bulletin for Histotechnology • Managing Editor, Gilles Lefebvre • Scientific Editor, Terri Staples, HT (ASCP), BS, HTL Vol. XXVIII, No. 1, May 1998 Normal Tissue Controls for Immunohistochemistry Kurt Nauss, HT, HTL, IHC (ASCP Qualification) Nathaniel Nauss, HT Centreville, VA 20120 In the field of immunohistochemistry, antibody underlined the control tissue or tissues on the control tissues are an important and necessary part antibody control list that we most frequently use for of the quality control of the reagents that are used a particular antibody. to determine if a tumor or other structure is positive or negative. Many pathologists prefer to Adrenal Synaptophysin, Chromogranin, use known positive tumor tissue as their positive Serotonin control, others prefer a sausage block control, and Appendix Serotonin, S100, HHF 35, Desmin, some use normal tissues that are known to contain SMA (Smooth Muscle Actin) the specific antigen that they are trying to identify. Bone Marrow Factor VIII, Myeloperoxidase, For many laboratories, obtaining specific tumor Glycophorine A controls can be a problem. We have compiled the following list of normal control tissues that can be Brain GFAP,Neurofilament used to test the reaction of the specific antibody in Colon AACT,AAT, BerEp4, CEA, immunohistochemistry procedures. Cam 5.2, AE- 1, AE I -AE3, KP1 (cd68), S100, SMA, Vimentin, This list is an accumulation of data from manu- MSA (HHF 35), Desmin, Factor facturers’ antibody specification sheets, our own VIII, HPL personal experiences, and research from manuals of microscopic anatomy, such as DiFiore’s, for tissues IN THIS ISSUE that contained the cells that were reported to react Normal Tissue Controls for Immunohistochemistry ……… 1 with the specific antibody. We have listed the tissues Rapid Demonstration of Lipofuscin with in alphabetical order. This is not a list of all of the Microwave Staining Methods ………………………………… 4 East Meets West ……………………………………………… 8 controls that can be used for these particular anti- New Headquarters Opened in Torrance …………………… 9 bodies but a quick reference list of normal tissue Just Grab the Tab …………………………………………… 11 that can be used to check for the positive reaction of Introducing Erin in Michigan and Ohio …………………… 11 the antibody being tested. I have also formulated a Every Staining Protocol – Automatically – at Any Time, in Any Order ………………………………… 12 list of control tissue by antibody that will allow the Stepping Up to Product Manager ……………………………… 14 technician to use different controls if one of the Histo-Logic® Back Issues other controls is not available. It is always important Becoming Available on www.sakuraus.com…………………16 to test an unknown tissue control before using that tissue as a control. For instance, if I normally use an ovarian carcinoma as a control tissue for the anti- No reader should utilize or undertake procedures in Histo-Logic articles unless the body “CA 125,” I would test the fallopian tube reader, by reason of education, training, and experience, has a complete understanding of the chemical and physical properties of all materials to be utilized control with the ovarian carcinoma to see if this type and the function of each material by which any procedure is accomplished. The of control was a satisfactory control for the patho- procedures discussed in these articles represent the opinions and experiences of the individual authors. Sakura Finetek U.S.A., Inc. assumes no responsibility or liability logist. The authors have not tested all of the tissues in connection with the use of any procedure discussed herein. that are listed for each antibody but we have © 1998 Sakura Finetek U.S.A., Inc. 1 Fallopian Tube CA125 list represents a small group of tissues that may be Kidney EMA, Collagen IV, Lysozyme used. Tumors and other pathological entities may also be used as long as the antigen/antibody Liver AFP,AAT, KP-1, CEA reaction is considered to be acceptable by the Pancreas AE-1, AE1-AE3, [BerH2 (Kl-1) in laboratory director. acinar cells], Chromogranin, AACT Tonsil, Colon, Skin Glucagon, Insulin, NSE, Pancreatic Polypeptide, Somatostatin, AAT Tonsil, Colon, Allelic nasal mucosa, Synaptophysin liver Pituitary ACTH, Follicle Stimulating AE- I Colon, Skin, Pancreas Hormone (FSH), Human Growth AE-3 Prostate Hormone (HGH), Luteinizing AE-1 / AE-3 Colon, Skin, Prostate, Pancreas Hormone (LH), Neurophysin, Prolactin, TSH ACTH Pituitary Placenta HPL, PLAP,B-HCG, HCG, AFP Fetal liver, Hepatoma Collagen IV BER H2 KI-1 Lymphoma, Pancreas Prostate AE3, AE1-AE, Desmin, Factor (KI-1) (CD30) VIII, HHF35 (muscle specific BCL2 Tonsil actin), HPCA (CD34), MA903, BEREP4 Small bowel, Colon PAP, PAS, SMA, Ulex, Vimentin BHCG Placenta Skeletal Myosin, Myoglobin, Sarcomeric Muscle actin Bombesin Stomach, Duodenum, Lung Skin AE 1, AE 1 -AE3, Collagen IV, Breast 2 Breast Ca, Normal Axilla, Apocrine Factor VIII, Factor XIIIa, KP-1, (GCDFP) sweat gland MIB-1, PCNA, S100, AACT, Breast 3 Breast Ca, Colon Ca, HPCA (CD 34), SMA (B72.3) Endometrial Ca, Small Bowel BerEP4, KP-1, S100, VIP Ovarian Ca, normal secretory (vasointestinal polypeptide) endometrium Spleen DBA-44 (hairy cell leukemia), CA125 Ovarian Ca, Fallopian Tube, Leu7, Myeloperoxidase, CEA Endocervix, Endometrium Stomach Gastrin, Bombesin CAM 5.2 Colon Testis Testosterone CEA Colon, Tonsil, Skin, Liver, Spleen Thyroid Calcitonin, Thyroglobulin Cathepsin D Breast Ca, Skin, Prostate, Liver Tonsil AACT,AAT, BCL2, CD43, Factor CERB 2 Breast Tumor, Gastric Ca, VIII, Factor XIIIa, HCG, HPCA Adeno Ca (CD 34), HPL, IgA, IgD, IgG, IgM, Kappa, Lambda, L26, LCA, Leu22, Leu7, LN-1, LN-2, Lysozyme, MIB-1, MAC387, Myeloperoxidase, MT-1, MT-2, Neutrophil Esterase, PCNA, PLAP,UCHL, Ulex. NOTE: Myoglobin, Myosin and Sarcomeric actin will react on tonsil if skeletal muscle is in the section that you are using as a control. Ureter AE1-AE3, Desmin, EMA, HHF35, SMA, Vimentin The following is a list of tissues that can be used as positive controls for the particular antibodies. This Fig 1. Collagen IV—Skin 3 100 2 Collagen IV Kidney, Skin, Placenta, Lymph HPL Placenta, Tonsil, Colon Node HPV Infected Tissue Chromogranin- Pancreas, Adrenal H Pylori Infected Tissue CD 43 Tonsil IgA Tonsil MV Infected Tissue IgD Tonsil Desmin Ureter, Prostate, Colon, Vas IgG Tonsil Deferens, Appendix IgM Tonsil DBA 44 Spleen, Node Insulin Pancreas EMA Kidney, Ureter, Breast, Glandular KP-1 Colon, Small Bowel, Liver, Skin Epithelium Kappa Tonsil Estrogen Breast Ca, or Normal Breast LEU22 Tonsil Epstein Barr Hodgkin’s Node Virus Lambda Tonsil Factor VIII Tonsil, Skin, Colon, Prostate, Bone LCA Tonsil Marrow L26 Tonsil Factor XIIIa Skin, Tonsil LN-1 Tonsil FSH Pituitary LN-2 Tonsil Gastrin Pyloric Mucosa (Stomach) Luteinizing Pituitary GFAP Glial Cells (Brain) Hormone Glucagon Pancreas LEU7 Tonsil, Spleen Glyco- LEU M1 Hodgkin’s Tissue phorine A Bone Marrow Lysozyme Tonsil, Renal Tubular Cells HbcAG Infected Tissue NM 903 Prostate HbsAG Infected Tissue MIB-1 Tonsil, Melanoma, Breast Ca, Skin SV I Infected Tissue MT- I Tonsil HSV II Infected Tissue MT-2 Tonsil Myelo- Bone Marrow, Spleen, Tonsil peroxidase MAC 387 Tonsil, Squamous Epithelium Myosin Skeletal Muscle, Tonsil, Tongue, Esophagus, Tonsil (if it has skeletal muscle) Myoglobin Heart, Tonsil (If it has skeletal muscle), Skeletal muscle Fig 2. Adenovirus—Infected Lung 3 200 HGH Pituitary HCG Placenta, Tonsil, Breast HHF35 (MSA) Ureter, Colon, Prostate, Vas Deferens, Appendix HMB45 Melanoma, Dysplastic Nevus HPCA (CD 34) Prostate, Tonsil, Skin Fig 3. Smooth Muscle Actin—Heart 3 200 3 NSE Pancreas, Pituitary, Colon, Brain Rapid Demonstration of Neurofilament Brain, Peripheral Nerve Lipofuscin with Microwave Neutrophil Tonsil ESTERASE Staining Methods PAP Prostate Stanley H. Shapiro, MS PCNA Skin, Tonsil, Lymphoma Fasahat Wasty, MD PSA Prostate Department of Laboratories and Pathology Pancreatic Head of the pancreas, Duodenum, Nassau County Medical Center Polypeptide Gastric Antrum East Meadow, NY 11554 PTH Parathyroid INTRODUCTION P53 Adeno Ca of Breast or Colon Lipofuscin, which is also referred to as lipopigment, PLAP Placenta, Tonsil, Colon Ca chromolipid, wear and tear pigment, and age Progesterone Breast Ca, or Normal Breast pigment, is a heterogeneous, autofluorescent cyto- plasmic organelle found in most animal tissues, Prolactin Pituitary especially in aging, metabolically active postmitotic PS2 Breast Ca tissue.1,2,3 It was first observed in human nerve cells Serotonin Appendix, Adrenal, Bronchial in 1842 and was first related to the aging process in Epithelium 1886.3 Lipofuscin at the ultrastructural level are S100 Small Bowel, Appendix, Skin, secondary lysozymes of the residual body type Melanoma, Colon, Nerve which vary in functional activity. They are indiges- tible, unexcreted cellular waste consisting primarily SMA Colon, Ureter, Prostate, Uterus, of intracellular membranes which normally accumu- Skin, Vas Deferens, Appendix lates progressively with age. It is hoped that rapid Sarcomeric Tongue,Tonsil (if it has skeletal identification of lipofuscin will facilitate its Actin muscle), Esophagus demonstration and ultimately assist in shedding light Somatostatin Pancreas on its role in physiologic and pathologic processes. Synaptophysin Adrenal, Pancreas Testosterone Testis TSH Pituitary Thyro- Thyroid, Papillary Ca globulin UCHL Tonsil ULEX Prostate, Tonsil, Endothelial Cells Vaso- Duodenum, Gastric Antrum, Small intestinal Intestine Polypeptide (VIP) Vimentin Ureter, Colon, Appendix, Prostate, Tonsil, Uterus References
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